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1.
Rev. bras. reprod. anim ; 47(3): 511-513, jul.-set. 2023.
Artigo em Português | VETINDEX | ID: biblio-1436633

Resumo

A criação comercial de gato doméstico tem aumentado assim como a realização de estudos para melhorar a eficiência reprodutiva é uma realidade. O interesse pela identificação das falhas reprodutivas tem crescido, entretanto, os casos de infertilidade nos machos são escassos na literatura disponível. O histórico de infertilidade de um gato deve ser investigado cuidadosamente, pois pode estar associado a mudanças no manejo ou doenças. O diagnóstico dessas 'falhas reprodutivas' geralmente é simples, se realizada uma boa anamnese, exames, físico e andrológico, minuciosos, assim como exames complementares, entre eles hemograma completo, perfil bioquímico e testes para doenças infecciosas como FIV, FELV e FIP.(AU)


Commercial breeding of domestic cats has increased, and studies to improve reproductive efficiency are a reality. Interest in identifying reproductive failures has increased, however, cases of infertility in men are scarce in the available literature. A cat's history of infertility should be investigated carefully as it may be associated with changes in management or illness. The diagnosis of these 'reproductive failures' is usually simple if a good anamnesis, thorough physical and andrological examinations are carried out, in addition to complementary tests, including complete blood count, biochemical profile, tests for infectious diseases such as FIV, FELV, FIP.(AU)


Assuntos
Animais , Masculino , Gatos , Infertilidade Masculina/diagnóstico , Técnicas de Laboratório Clínico/veterinária
2.
Acta sci. vet. (Impr.) ; 51: Pub. 1922, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1443987

Resumo

Background: Enzootic bovine leukosis (EBL) is a widespread infectious disease caused by the bovine leukemia virus (BLV), which results in immune system dysfunction. The resulting immunosuppression may lead to an increased prevalence of other diseases. Dairy cows infected have altered immune function associated with decreased milk production and shortened lifespan and decreased immune response to immunization. BLV infection, however, is often asymptomatic, so any connection between subclinical infection and common reproductive diseases remains unknown. This study aimed to describe the relationship between naturally occurring subclinical BLV and infectious reproductive diseases seroconversion in the field. Materials, Methods & Results: The diseases investigated included Bovine viral diarrhoea (BVD), Bovine alfaherpesvirus 1 (BoHV-1), Bovine gammaherpesvirus 4 (BoHV-4), Chlamydiosis, Leptospirosis, Brucellosis and Neosporosis in dairy cattle. Six hundred fifty-five sera samples from the northern and south-central regions of Uruguay, from asymptomatic female Holstein and Holstein crosses without a history of vaccination against reproductive diseases were processed using reference diagnostic methods (Seronautalization, ELISA, MAT, Rose Bengal Plate test). The seroprevalence of BLV was 20.0%. Seroprevalence of reproductive diseases BVD, BoHV-1 and BoHV-4 were 99.3%, 41.2% and 27.3% of the populations, respectively, and the total seroprevalence of Leptospirosis, bovine Neospora caninum and Chlamydiosis were 19.8%, 29.8% and 33.0% respectively. The results revealed positive associations between naturally contracted BLV and the presence of antibodies against BoHV-1 (P = 0.002), as well as between naturally contracted BLV and presence of antibodies against Leptospira spp. (P = 0.028). Discussion: BLV infection can impact innate and adaptive immune system cells and alter the proper functioning of uninfected cells. BLV infection may also induce changes in the complex balance of cytokine expression, cell proliferation, and programmed cell death in T- and B-lymphocytes, which is critical for immune competence and effective response to infectious challenges. The progression of BLV infection has a substantial effect on host defense mechanisms. Indeed, lowmagnitude serologic responses to a commercial foot-and-mouth disease vaccine and a J5 Escherichia coli vaccine have been observed. These results are supported by recent trial studies showing a reduced immune response to vaccination against BoHV-1 and Leptospira spp. in asymptomatic animals infected with BLV. These are 2 of the most prevalent infectious reproductive diseases in cattle worldwide, and our results provide evidence that a link between BLV and susceptibility to these diseases may exist. Although there is evidence of the co-occurrence of these diseases, it remains unknown whether there is a direct or indirect effect of BLV on infertility, embryonic loss, or abortion. Another possibility is that natural infection with these reproductive pathogens (BoHV-1, Leptospira, or others) promotes BLV expression, negatively affecting the farms where these pathogens are endemic. Considering the high seroprevalence of BLV in dairy herds in North and South America where the infection is endemic, it was explored BLV's role as an immunosuppressant by quantifying its co-occurrence with diseases that affect reproductive performance in breeding herds. Future work should clarify the role of BLV and the co-occurring pathogens in causing infertility or abortions.


Assuntos
Animais , Feminino , Bovinos , Leucose Enzoótica Bovina/complicações , Vírus da Leucemia Bovina , Doenças dos Genitais Femininos/veterinária , Rinotraqueíte Infecciosa Bovina , Leptospirose/veterinária , Infecções do Sistema Genital/veterinária
3.
Anim. Reprod. (Online) ; 20(2): e20230021, 2023. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1435535

Resumo

Radiotherapy causes destruction of tumor cells, but also threatens the integrity and survival of surrounding normal cells. Then, woman submitted to irradiation for cancer treatment may present permanent ovary damage, resulting in impaired fertility. The objective of this study was to investigate the effects of therapeutic doses of ionizing radiation (IR), used for ovarian cancer treatment in humans, on bovine cumulus-oocyte complexes (COCs) as experimental model. Bovine ovaries were exposed to 0.9 Gy, 1.8 Gy, 3.6 Gy or 18.6 Gy IR, and then COCs were collected and used to evaluate: (a) oocyte nuclear maturation; (b) presence of phosphorylated H2A.X (γH2AX), as an indicator of DNA double-strand breaks (DSBs); and (c) expression of genes involved in DNA repair (TP53BP1, RAD52, ATM, XRCC6 and XRCC5) and apoptosis (BAX). The radiation doses tested in this study had no detrimental effects on nuclear maturation and did not increase γH2AX in the oocytes. However, IR treatment altered the mRNA abundance of RAD52 (RAD52 homolog, DNA repair protein) and BAX (BCL2-associated X protein). We conclude that although IR doses had no apparent effect on oocyte nuclear maturation and DNA damage, molecular pathways involved in DNA repair and apoptosis were affected by IR exposure in cumulus cells.(AU)


Assuntos
Animais , Feminino , Bovinos , Oócitos/citologia , Radiação Ionizante , Dano ao DNA , Perfilação da Expressão Gênica/veterinária
4.
Rev. bras. reprod. anim ; 46(4): 409-412, out.-dez. 2022. tab
Artigo em Espanhol | VETINDEX | ID: biblio-1415224

Resumo

La prevalencia de la brucelosis canina por B. canis, está aumentando a nivel mundial, al igual que los reportes en personas. La ausencia de vacunas y de programas de control, permiten predecir una rápida expansión de la enfermedad, con el consecuente impacto en la reproducción canina y en la Salud Pública. Por lo anterior, es urgente analizar candidatos vacunales que consideren las características genómicas de las cepas circulantes a nivel mundial, toda vez que se ha comprobado variabilidad genética, particularmente en genes asociados a factores de virulencia tales como proteínas de membrana externa y sistema de secreción tipo IV. La presencia de un conglomerado genético de cepas Latinoamericanas de B. canis debe ser considerado en estudios futuros de tal manera de generar productos vacunales sin limitaciones geográficas.(AU)


A prevalência da brucelose canina por B. canis está aumentando em todo o mundo, assim como os relatos em humanos. A falta de vacinas e programas de controle prevêem uma rápida disseminação da doença, com conseqüente impacto sobre a reprodução canina e a saúde pública. Portanto, é urgente analisar os candidatos a vacina que consideram as características genômicas das cepas que circulam pelo mundo, uma vez que a variabilidade genética foi comprovada, particularmente nos genes associados a fatores de virulência, como as proteínas da membrana externa e o sistema de secreção tipo IV. A presença de um grupo genético de linhagens latino-americanas de B. canis deve ser considerada em estudos futuros a fim de gerar produtos vacinais sem limitações geográficas.(AU)


Assuntos
Variação Genética , Brucelose/prevenção & controle , Infertilidade/veterinária , Reprodução/genética , Brucella canis/genética , Desenvolvimento de Vacinas/métodos
5.
Rev. bras. reprod. anim ; 46(2): 154-160, Abril-Junho 2022. ilus, tab
Artigo em Português | VETINDEX | ID: biblio-1378120

Resumo

As disfunções hormonais podem interagir com eixo hipotalamo-hipófise-gônadas (H/H/G) resultando em alteração da espermatogênese e impactando negativamente a fertilidade dos machos. As endocrinopatias na espécie canina são mais frequentes nas fêmeas e muitas vezes os sinais clínicos iniciais envolvem alopecias e alterações de peso, comportamento e de metabolismo. As disfunções hormonais comuns em cães machos incluem o hipotireoidismo, hiperplasia prostática benigna e os tumores testiculares. Cães idosos geralmente apresentam alterações hormonais dependentes da idade, mas a literatura é escassa e conflitante em relação a esta condição, assim o objetivo dessa revisão é informar e atualizar conceitos relacionados às disfunções hormonais que podem causar infertilidade em cães machos com vistas a ferramentas mais modernas de diagnósticos e avaliação seminal.(AU)


Hormonal dysfunctions may interact with hypothalamus, pituitary, gonads (H/H/G) axis resulting in altered spermatogenesis and negatively impacting male fertility. Endocrinopathies in the canine species are more frequent in females and often the initial clinical signs involve alopecia and changes in weight, behavior, and metabolism. Common hormonal dysfunctions in male dogs include hypothyroidism, benign prostatic hyperplasia, and testicular tumors. Older dogs usually present age- dependent hormonal changes, but the literature is scarce and conflicting regarding this condition, thus the aim of this review is to inform and update concepts related to hormonal dysfunctions that can cause infertility in male dogs with a view to more modern diagnostic tools and seminal evaluation.(AU)


Assuntos
Animais , Masculino , Hiperplasia Prostática/diagnóstico , Neoplasias Testiculares/diagnóstico , Cães/fisiologia , Doenças do Sistema Endócrino/diagnóstico , Hipotireoidismo/diagnóstico , Próstata , Fenômenos Reprodutivos Fisiológicos , Infertilidade/veterinária
6.
Anim. Reprod. (Online) ; 19(4): e20230007, 2022. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1420060

Resumo

At the time of its discovery and characterization in 1994, leptin was mostly considered a metabolic hormone able to regulate body weight and energy homeostasis. However, in recent years, a great deal of literature has revealed leptin's pleiotropic nature, through its involvement in numerous physiological contexts including the regulation of the female reproductive tract and ovarian function. Obesity has been largely associated with infertility, and leptin signalling is known to be dysregulated in the ovaries of obese females. Hence, the disruption of ovarian leptin signalling was shown to contribute to the pathophysiology of ovarian failure in obese females, affecting transcriptional programmes in the gamete and somatic cells. This review attempts to uncover the underlying mechanisms contributing to female infertility associated with obesity, as well as to shed light on the role of leptin in the metabolic dysregulation within the follicle, the effects on the oocyte epigenome, and the potential long-term consequence to embryo programming.(AU)


Assuntos
Animais , Feminino , Leptina/análise , Obesidade Materna/veterinária , Infertilidade Feminina/diagnóstico , Epigenômica/métodos
7.
Anim. Reprod. (Online) ; 19(3): e20220039, set. 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1393233

Resumo

Associations of the activity of the paraoxonase 1 (PON1) enzyme with boar sperm quality still needs to be characterized, since boar ejaculates present distinct portions with differences in sperm concentration and quality. This study evaluated PON1 activity in the serum, in the distinct portions of boar ejaculates and estimated correlations with sperm quality parameters. Ejaculates and blood samples were collected from six boars for three weeks (two per week per boar; n = 36). Serum and post-spermatic portion PON1 activities were positively correlated (P = 0.01) but were both uncorrelated with the PON1 activity in the sperm-rich portion and in the whole ejaculate (P > 0.05). Differences in PON1 activity among boars were only observed in the sperm-rich portion of the ejaculate (P < 0.05). The PON1 activity in the serum and in the post-spermatic portion was generally negatively correlated with parameters of spermatozoa kinetics (P < 0.05). In the sperm-rich portion, PON1 activity was positively correlated with sperm concentration (P < 0.0001), curvilinear distance and velocity (both P < 0.05) and DNA integrity (P < 0.05), but negatively correlated with straightness and linearity (P < 0.05). Thus, boar ejaculates with increased PON1 activity in the sperm-rich portion may present increased concentration and spermatozoa with acceptable curvilinear velocity and distance and DNA integrity, which suggests that PON1 activity may be a biomarker for potential fertility.(AU)


Assuntos
Animais , Masculino , Sus scrofa/fisiologia , Arildialquilfosfatase/análise , Análise do Sêmen/veterinária , Biomarcadores , Fármacos para a Fertilidade/análise , Dieta Rica em Proteínas/veterinária
8.
Acta sci. vet. (Impr.) ; 50: Pub. 1895, 2022. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1414923

Resumo

Background: Buffalo breeding is common in many countries. Buffalo's milk is used in the production of mozzarella, yoghurt, ice cream, and various dairy desserts; meat is preferred in sausage production. The female buffaloes are bred to benefit from their milk and to obtain offspring. These animals, which are not suitable for feeding in barns, generally live in pastures, especially on wet land, and are very difficult to follow. Therefore, diseases occur randomly in slaughterhouses. Studies on genital system problems are very limited. Water buffaloes (Bubalus bubalis) are animals with economic value as many buffalo products are provided. In this respect, inspection and control of female genital disorders is fundamental to ensure good reproductive performance of female buffaloes. The aim of the study was to investigate pathomorphological lesions occurring in the ovaries of water buffaloes which were sent to slaughterhouses. Materials, Methods & Results: A total of 198 ovaries of water buffaloes were collected from various slaughterhouses located in Adapazari, Istanbul, Kocaeli, Samsun and Trabzon cities of Turkey. After macroscopic examination; tissue samples were fixed in 10 % buffered formalin, processed routinely and were stained with hematoxylin-eosin (HE). As a histochemical staining, Masson's trichrome staining was applied to characterize the lesions. Immunohistochemistry (IHC) was performed on 10 % neutral formalin fixed, paraffin-embedded, 4-6-µm-thick sections from ovaries using progesterone receptor (PR) with streptavidin-biotin complex peroxidase (StrepABC-P) method. Histopathologically, follicular cysts (n:147) and luteal cysts (n:22) were seen. While the cyst lumens were sometimes surrounded by granulosa and/or luteal cells, most of them were limited by the connective tissue capsule structure. This capsule structure was shown in blue by Masson's trichrome staining. Hemorrhage was observed in some cystic corpus luteum, diagnosed as corpus hemorrhagicum. In addition, cysts giving papillary extension into the lumen and inflammation of some ovaries were observed. Immunohistochemically, the staining with PR antibody in ovarian cysts showed no immunolabelling around the follicular cyst, while the nuclei of some of the luteal cells forming the luteal cyst had strongly nuclear positivity and slightly cytoplasmic positivity. In the biochemical examination of the fluids obtained from cystic ovaries (n: 37), the average of estradiol was 2.84 ng/mL (min: 0.01 ng/mL, max: 4.30 ng/mL) and progesterone average is 49.09 ng/mL (min: 1.88 ng/mL, max: 254.2 ng/mL). Discussion: Ovarian cysts in buffaloes seem to be among the serious fertility problems as in cattle. Although the exact cause of ovarian cyts has not been determined yet, it is known that the hypothalamic-pituitary-gonadal (HPG) axis is disrupted in cyst formation in general. In this study, the mean estradiol value was within the standard range but close to the lower limit; progesterone value was above the limit. The increase in progesterone level was compatible with the pathogenesis of cyst genesis. Beside this result, staining with PR was positive in the luteal cells that formed the luteal cyst immunohistochemically. In addition, although the hemorrhages observed in the corpus luteum are considered physiological, it should not be forgotten that they can be vital if they rupture. All these results show us the animals sent for slaughtering mostly have serious genital problems threatening their fertility.


Assuntos
Animais , Feminino , Cistos Ovarianos/veterinária , Ovário/lesões , Búfalos/lesões , Imuno-Histoquímica/veterinária , Infertilidade Feminina/prevenção & controle
9.
Rev. bras. reprod. anim ; 46(4): 435-437, out.-dez. 2022.
Artigo em Inglês | VETINDEX | ID: biblio-1415235

Resumo

Assisted Reproductive Technologies (ART) are currently used in animals usually in three main situations: 1. As a form of treatment of subfertility and infertility in females and/or males, 2. As a method to obtain genetically valuable progeny in relatively short time in healthy fertile animals, 3. As a modern smart tool to obtain progeny in endangered animal species in programs of rescue of wild animals threatened extinction. Generally the efficiency of reproductive biotechniques in dogs and cats is lower in comparison to obtained in farm animals and in human. Independently of the aim of their use, there are some techniques, which are better developed in dogs and some others seems to be better developed in cats. It may be assumed that simple, clinical techniques are well elaborated and more frequently used in dogs while more advanced techniques are better developed in cats. The level of effectiveness of ART is conditioned by anatomical and physiological factors specific for species, general demands for their use in veterinary practice and research, and general interest of breeders and scientific community in such activity.(AU)


Assuntos
Animais , Fertilização in vitro/veterinária , Gatos , Técnicas de Reprodução Assistida/veterinária , Cães , Fertilidade
10.
Arq. bras. med. vet. zootec. (Online) ; 74(2): 245-252, Mar.-Apr. 2022. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1374415

Resumo

The aim of the present study is to describe the surgical technique and complications of uterine elevation by laparoscopic surgery, using the broad uterine ligament plication with toogles. Five mares of different breeds, weight and age were selected, presenting poor perineal conformation and pendulous uterus. The surgical technique was performed with a toogle applicator, 0.6mm sterile nylon thread and toogles, using three portals on each side of the flank. After one month, a new laparoscopy was performed, with only one portal on each side, to observe uterine positioning and healing. The average surgical time reached in this study was 82 minutes per procedure, and the uterus of all mares was successfully raised, remaining in a more horizontal orientation and presenting satisfactory healing. There are only two articles published with uteropexy in mares, where the proposed techniques take longer, which consequently increases the surgical risk.


O objetivo do presente estudo é descrever a técnica cirúrgica e as complicações da elevação uterina por cirurgia laparoscópica, utilizando-se a plicatura do ligamento uterino com aplicação de toogles. Foram selecionadas cinco éguas de diferentes raças, peso e idade, as quais apresentavam baixa conformação perineal e útero pendular. A técnica cirúrgica foi realizada com aplicador de toogles, fio de nylon estéril de 0,6mm e toogles, sendo utilizados três portais de cada lado do flanco. Após um mês, uma nova laparoscopia foi realizada, com apenas um portal de cada lado, para observar o posicionamento uterino e a cicatrização. O tempo cirúrgico médio alcançado neste estudo foi de 82 minutos por procedimento, e o útero de todas as éguas foi elevado com sucesso, permanecendo em uma orientação mais horizontal e apresentando cicatrização satisfatória. Existem apenas dois artigos publicados com a utilização da uteropexia em éguas, porém nestes as técnicas propostas demoram mais, o que, consequentemente, aumenta o risco cirúrgico.


Assuntos
Animais , Feminino , Útero/cirurgia , Laparoscopia/métodos , Cavalos , Laparoscopia/veterinária
11.
Acta sci. vet. (Impr.) ; 50: Pub. 1898, 2022. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1414959

Resumo

Background: Blue tongue (BT) is a noncontagious viral disease transmitted by hematophagous arthropods, especially of the genus Culicoides. The economic impact of the disease is related not only to deaths in sheep herds but also to the possible correlation of virus infection with the development of other diseases, such as pneumonia, abortion and movement problems. The economic losses caused by Blue Tongue are linked to restrictions on the import and export of animals and their genetic material and to the reproductive disorders associated with this disease. In addition, the fact that cattle take the role of reservoir, combined with the care by other countries with outbreaks of infection and biological contamination of their products, hinders trade in Mercosul, United States and Europe. Cattle are affected by Blue Tongue Virus in endemic areas and in some epidemic areas, but the development of clinical disease is rare. The clinical signs, when evident, range from reproductive losses, such as embryonic death, abortion, fetal malformation, temporary sterility, infertility in bulls, stillbirths and the birth of weak animals. The objective of this study was to determine the epidemiological aspects of Blue Tongue Virus (BTV) infection in dairy cattle in the Lavras region, state of Minas Gerais, Brazil. Materials, Methods & Results: A cross-sectional study was conducted to evaluate the frequency of cattle and herds seropositive for Blue Tongue in the southern region of Minas Gerais. In this study, 54 dairy farms were visited. A total of 586 serum samples were collected from cows of reproductive age. Sampling was random, and serum samples were collected from lactating cows over 24 months of age by puncture of the jugular vein and/or coccidian vein. The samples were transported and stored at the Setor de Patologia Veterinária, at the Universidade Federal de Lavras (SPV-UFLA), where they were centrifuged, and the serum aliquots were obtained, transferred to microtubes and kept at -20°C until the serological tests were performed. The samples were tested with the agarose gel immunodiffusion test (AGID) for anti-blue tongue virus antibodies. The AGID test is more practical and is the main method used to identify Blue Tongue Virus seroprevalence in different ruminant species. They are considered important tools for epidemiological surveillance of the disease. A prevalence of 83.28% was observed among animals that were seropositive for Blue Tongue Virus (488/586; IC 95% = 80.0 - 86.21). In addition, 100% (54/54; IC 95% = 93.4 - 100.0) of the farms had at least 1 positive animal, with rates ranging from 45.45% to 100% within the herds and where 22.22% of the farms had rates of 100% of the animals being positive. Discussion: Blue Tongue is a disease known to affect domestic and wild ruminants in Brazil. However, there is a lack of more precise information about its epidemiology and occurrence in the country and of joint efforts of researchers, producers and the government to understand in detail both the biology of vectors and the viral biology of Blue Tongue Virus in Brazil. This is the first record of detection of anti-blue tongue virus antibodies in cattle in the southern region of Minas Gerais. The results suggest that Blue Tongue Virus is present in cattle in the study area.


Assuntos
Animais , Bovinos , Reoviridae/isolamento & purificação , Orbivirus/isolamento & purificação , Bluetongue/epidemiologia , Testes Sorológicos/veterinária , Imunodifusão/veterinária
12.
Rev. bras. reprod. anim ; 46(4): 369-372, out.-dez. 2022.
Artigo em Inglês | VETINDEX | ID: biblio-1415071

Resumo

Male dog fertility disorders are usually troublesome and challenging for a practicing veterinarian. It may be generally assumed, that reproductive potential in this species is lower than in farm animals and it is still decreasing. This situation starts to be similar to human medicine, where we observe dramatic drop of reproductive capacity, which resulted in the need of implementation of Assisted Reproductive Technologies (ART). Situation in dogs is more complicated owing the fact, that the use of ART meets many obstacles. Low fertility potential in dogs appears to be the result of variable factors such as: specific criteria of selection for reproduction in which fertility performance in not a priority, lack of periodical obligatory fertility check, species specific predisposition for many reproductive diseases and no age limit for reproductive use of males. Dogs are kept in human environment and exposition for civilizational byproducts influences negatively not only on our health, but also on health our 'minor brothers'. It should be bear in mind, that reproductive organs are very sensitive for environmental factors disrupting homeostatic balance. The decline in male dog fertility over the past decades was proved, with potential link to environmental contaminants (4). They were found in pet foods and were also detected in the sperm and testes of adult dogs causing a detrimental effects on sperm function. Over the 26 years of the study of Lea et al. (4), authors found a decrease in the percentage of normal motile sperm. Between 1988 and 1998, sperm motility declined by 2.5 per cent per year. Then from 2002 to 2014 sperm motility continued to decline at a rate of 1.2% per year. In addition, the male pups had an increased incidence of cryptorchidism. Basics of physiology of reproduction of male dogs. Normally the puberty in males is associated with presence of normal sperm cells in genital organs. It is reached in male dogs at age around 5-6 months. Such a young dog obviously cannot be used for reproduction. Reproductive maturity is associated later, with development of normal sexual behavior and production of sufficient number of normal, fertilizing competent spermatozoa. It corresponds with 12-18 months of animal age. Testicular descent is completed usually before weaning period, but sometimes testicles may reach scrotum later, but never after the end of 6 month of age. That time inguinal canals start to be so narrow, that caudal passing of gonads is unlikely. Male dogs have only one accessory sexual gland - prostate, which produces vast portion of seminal plasma.(AU)


Assuntos
Animais , Masculino , Cães/fisiologia , Fertilidade/fisiologia , Técnicas de Reprodução Assistida/veterinária , Andrologia/métodos , Fenômenos Reprodutivos Fisiológicos
13.
Rev. bras. reprod. anim ; 46(2): 161-169, Abril-Junho 2022. tab
Artigo em Português | VETINDEX | ID: biblio-1378127

Resumo

Falha da fertilidade nas espécies canina e felina é uma das principais preocupações entre criadores e a busca por tratamentos específicos é uma constante. Assim, o uso de substâncias com poder antioxidante como vitaminas, minerais, flavonoides, ácidos graxos, etc. são requeridos em tratamentos para melhorar índices reprodutivos, tais substâncias são classificadas como nutracêuticos. Ação antioxidante se faz pertinente ao combate do estresse oxidativo proveniente dos radicais livre e peroxidação lipídica produzidos durante a espermatogênese e em casos de sub ou infertilidade. O desequilíbrio entre as ações oxidativas e antioxidativas acarreta diminuição dos parâmetros seminais.(AU)


Fertility failure in canine and feline species is one of the main concerns among breeders and the search for specific treatments is a constant. Thus, the use of substances with antioxidant power such as vitamins, flavonoids, fatty minerals, etc. are necessary in treatments to improve reproductive indices, such substances are classified as nutraceuticals. The antioxidant action is relevant to combat oxidative stress from free radicals and lipid peroxidide during spermatogenesis and in cases of sub or infertility and antioxidative as oxidative activities in seminal tissues.(AU)


Assuntos
Animais , Gatos , Cães , Peroxidação de Lipídeos/fisiologia , Estresse Oxidativo/fisiologia , Suplementos Nutricionais/efeitos adversos , Análise do Sêmen/veterinária , Fármacos para a Fertilidade Masculina/química , Antioxidantes/efeitos adversos , Espermatogênese/fisiologia , Vitaminas/efeitos adversos , Flavonoides/química , Ácidos Graxos/química , Minerais/química
14.
Acta sci. vet. (Impr.) ; 49: Pub, 1834, 2021. ilus, tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1363710

Resumo

Pregnancy losses are a major concern in livestock industry due to their economic impact on producers. Campylobacter fetus subspecies fetus (Cff) and C. fetus subspecies venerealis (Cfv) are directly related to reproductive failures in ruminants. Cff colonizes the gastrointestinal tract of a wide range of hosts leading to abortion, while Cfv is restricted to genital tract being generally associated to infertility in bovine. Considering the great economic losses related to campylobacteriosis in cattle and ovine herds, this study aims to investigate the occurrence of C. fetus, considering Cff and Cfv subspecies, in bovine and ovine spontaneously aborted fetuses in state of Rio Grande do Sul, Brazil. In this study, samples of abomasal fluid collected from 30 spontaneously aborted bovine (n = 18) and ovine (n = 12) fetuses were investigated for the detection of Campylobacter fetus throughout conventional PCR. Positive fetuses for C. fetus presence were further analyzed by molecular assays for Cff and Cfv detection, in order to determine subspecies identification. When available, samples of the main organs of the thoracic and abdominal cavities, as well as the brain, skeletal muscle, eyelid, skin, and placenta were collected for further histopathological analyses and bacterial culture, aiming to assess the presence of infection lesions and pathogens in those sites, respectively. Additionally, RT-qPCR assays were also performed for the detection of ruminant pestivirus, in order to detect bovine viral diarrhea cases. Throughout the present methodology, C. fetus was detected in the abomasal fluid samples of 2 bovine fetuses, being both identified as Cfv subspecies by PCR. Histopathological analyses demonstrated that macroscopic and microscopic changes found in the Cfv-positive animals were not either specific or directly related to Campylobacter infections. Moreover, no significant bacterial growth was observed in microbiological culture from the collected tissues, and both fetuses were negative for ruminant pestivirus. Differently, there was no detection of C. fetus in any of the analyzed ovine fetuses. Considering that abortion diagnosis rates reported in cattle and sheep industry are highly variable among the published studies, and that abortion diagnoses are commonly inconclusive due to difficulties in sampling methodology and inadequate identification of the pathogen involved, it is important to investigate the etiological causes of abortion the herds for better understanding the causes of pregnancy issues and monitoring their occurrence. In addition, the absence of pathognomonic lesions in the tissues investigated in the histopathological analyses observed in this study strongly suggests that well-known etiological agents commonly associated to abortion, such as Leptospira spp., Toxoplasma spp., Chlamydia spp. and Neospora caninum, are unlikely to be the cause of infection of the analyzed fetuses. Taking this into account, the presence of C. fetus in the abomasal fluid samples from two bovine fetuses demonstrated in the present study suggests the possible association of Cfv not only with infertility, but also with cases of bovine abortion, highlighting the importance of investigating unusual causal agents of abortions in sheep and cattle. Overall, an adequate diagnosis is essential for establishing better prevention strategies to avoid the circulation of abortion-related infectious agents in the herds.(AU)


Assuntos
Animais , Feminino , Gravidez , Campylobacter fetus , Infecções por Campylobacter/veterinária , Aborto Animal , Infertilidade/veterinária , Criação de Animais Domésticos/economia , Ruminantes
15.
Rev. bras. reprod. anim ; 45(4): 286-295, out.-dez. 2021. tab
Artigo em Português | VETINDEX | ID: biblio-1492671

Resumo

A mudança de luminosidade claramente possui um grande impacto no ciclo reprodutivo em diferentes espécies, principalmente espécies reprodutoras sazonais. Apesar de o suíno doméstico ser capaz de se reproduzir com sucesso no decorrer do ano, os efeitos da sazonalidade têm sido estudado por diversos anos visto que, ainda hoje, existem reduções de taxas reprodutivas em leitoas e porcas, principalmente nas épocas de verão e outono. Além dos efeitos do estresse por calor que acometem granjas durante o verão, efeitos do fotoperíodo podem ser identificados em dinâmicas hormonais, e alguns resultados de desempenho dependendo das estações do ano. Há indícios que o fotoperíodo em associação com o estresse causado pelas altas temperaturas do ambiente sejam as principais causas do processo de infertilidade sazonal em suínos. Estudos mais recentes demonstram efeitos negativos na fertilidade de leitoas e primíparas quando expostas a ambientes com baixa luminosidade durante o período de cobertura. Apesar de ainda existir grande espaço para mais investigações a respeito dos efeitos de duração e intensidade do fotoperíodo artificial e para melhorias em termos de ambiência, os órgãos responsáveis pela regulamentação de animais de produção sugerem uma intensidade e tempo mínimo de exposição à iluminação artificial para os suínos em confinamento.


The changes in photoperiod clearly impact the reproductive cycle in different species, especially in seasonal breeders. Although the domestic pig can successfully reproduce throughout the year, the seasonal effects have been studied for years, and still today, reduction in reproductive rates exist in gilts and sows, mainly during summer and fall periods. Besides the high temperatures during summer causing detrimental effects due to heat stress in swine, changes in photoperiod effects can be identified by hormonal changes and performance results depending on the season. There is evidence of photoperiod associated with heat stress being the leading causes of seasonal infertility in swine. Recent studies demonstrate adverse effects on gilts and primiparous sows fertility when exposed to an environment with low lighting intensity during the breeding period. Although further investigation on the effects of intensity and duration of artificial photoperiod and improvements in terms of ambient is still needed, the guidelines and regulations for animal production suggest a minimum exposure of artificial light intensity and duration for confined swine.


Assuntos
Feminino , Animais , Fotoperíodo , Infertilidade/veterinária , Suínos/fisiologia , Técnicas Reprodutivas
16.
Rev. bras. reprod. anim ; 45(4): 426-429, out.-dez. 2021.
Artigo em Inglês | VETINDEX | ID: biblio-1492691

Resumo

Endometritis refers to an inflammation of the uterine mucosa that does not extend beyond the stratum spongiosum. Recent studies indicate it is a common finding in 30-50% of bitches suffering from infertility/subfertility. This disorder is subclinical, and its diagnosis involves sampling the uterus, whether it is by performing a cytology from flushing the organ or histology on collected uterine biopsies. Its pathophysiology remains unclear in 2021. Some cases are associated with cystic endometrial hyperplasia, which leads to disruption in the uterine clearance mechanisms after breeding ; while other appear as a pure inflammatory process. While we don’t have all the answers yet, there is no doubt today that these disorders must be included in the differential diagnosis of infertility in the bitch. The work that has already been done on this topic already offers some idea on how to approach these cases in our veterinary clinics.


Assuntos
Feminino , Animais , Cães , Doenças Uterinas/diagnóstico , Endometrite/diagnóstico , Endometrite/veterinária , Infertilidade/diagnóstico
17.
Acta sci. vet. (Impr.) ; 49: Pub.1812-2021. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1458451

Resumo

Background: Bluetongue is a vector-borne viral disease transmitted by midges from the genus Culicoides. The disease caninfect most of the ruminant and camelid species, but the severe disease is most often seen in european wool and muttonsheep breeds. In this sense, there is a gap in the knowledge on BTV infection in hair sheep breeds from tropical zones.Thus, this study aimed at establishing whether exposure to BTV is a risk factor for reproductive failure in Santa Inês ewes,a hair sheep breed, reared under tropical conditions in Brazil.Materials, Methods & Results: A retrospective cross-sectional study was carried out in sheep farms in São Paulo state,Brazil, after the rainy season. Serum samples from 110 Santa Inês ewes with a history of reproductive disorders, in the last6 months, which were included: abortion, premature birth, stillbirth, retention of placenta, infertility, estrus repetition, fetalmalformation, weak lamb birth and neonatal death were collected. The presence of antibodies against BTV was assessedby agar gel immunodiffusion method (AGID). Serology to the infectious agents Brucela ovis, Lepstopira spp., Toxoplasmagondii, Neospora caninum and Campylobacter sp. were also assessed. Bivariate associations between the outcome andindividual explanatory variables were assessed using the Fisher’s exact test. Abortion was the most common reproductive disorder (53%; 74/139) observed, followed by estrus repetition (12%; 17/139) and infertility (11%; 15/139). Otherdisorders related to the conceptus totaled nearly one fourth of the reported disorders. A total of 20% (22/110) of the eweswere seropositive to BTV. A higher frequency of BTV seropositive than BTV seronegative ewes with a history of abortionwas found. Also, abortion with seroreactivity to BTV was tested for prevalence ratio that showed 1.38 [95% CI 1.10-1.74;P = 0.030]. With regards to the abortion involvement of other infectious diseases associated with the seropositive ewes to...


Assuntos
Feminino , Animais , Gravidez , Aborto Animal/virologia , Bluetongue , Vírus Bluetongue , Fatores de Risco , Infertilidade Feminina/veterinária , Ovinos/virologia
18.
Acta sci. vet. (Online) ; 49: Pub. 1812, May 28, 2021. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-30584

Resumo

Background: Bluetongue is a vector-borne viral disease transmitted by midges from the genus Culicoides. The disease caninfect most of the ruminant and camelid species, but the severe disease is most often seen in european wool and muttonsheep breeds. In this sense, there is a gap in the knowledge on BTV infection in hair sheep breeds from tropical zones.Thus, this study aimed at establishing whether exposure to BTV is a risk factor for reproductive failure in Santa Inês ewes,a hair sheep breed, reared under tropical conditions in Brazil.Materials, Methods & Results: A retrospective cross-sectional study was carried out in sheep farms in São Paulo state,Brazil, after the rainy season. Serum samples from 110 Santa Inês ewes with a history of reproductive disorders, in the last6 months, which were included: abortion, premature birth, stillbirth, retention of placenta, infertility, estrus repetition, fetalmalformation, weak lamb birth and neonatal death were collected. The presence of antibodies against BTV was assessedby agar gel immunodiffusion method (AGID). Serology to the infectious agents Brucela ovis, Lepstopira spp., Toxoplasmagondii, Neospora caninum and Campylobacter sp. were also assessed. Bivariate associations between the outcome andindividual explanatory variables were assessed using the Fishers exact test. Abortion was the most common reproductive disorder (53%; 74/139) observed, followed by estrus repetition (12%; 17/139) and infertility (11%; 15/139). Otherdisorders related to the conceptus totaled nearly one fourth of the reported disorders. A total of 20% (22/110) of the eweswere seropositive to BTV. A higher frequency of BTV seropositive than BTV seronegative ewes with a history of abortionwas found. Also, abortion with seroreactivity to BTV was tested for prevalence ratio that showed 1.38 [95% CI 1.10-1.74;P = 0.030]. With regards to the abortion involvement of other infectious diseases associated with the seropositive ewes to...(AU)


Assuntos
Animais , Feminino , Gravidez , Vírus Bluetongue , Bluetongue , Aborto Animal/virologia , Ovinos/virologia , Infertilidade Feminina/veterinária , Fatores de Risco
19.
Rev. bras. reprod. anim ; 45(4): 492-499, out.-dez. 2021.
Artigo em Português | VETINDEX | ID: biblio-1492700

Resumo

A endometrite é a principal causa de infertilidade em éguas, além de gerar grandes transtornos econômicos e produtivos. Objetivou-se com esse trabalho, realizar uma revisão sistemática sobre endometrite equina, sua etiologia, os mecanismos próprios de defesa uterina mecânicos e quimiotáticos. Avaliando ainda os principais microrganismos envolvidos no processo bem como sua virulência e resistência antimicrobiana. Apresentamos ainda uma proposta de abordagem diagnóstica contemplando a identificação dos agentes, susceptibilidade farmacológica associadas a comprovação da produção de biofilme, assim como sua graduação, possibilitando assim em caso de comprovação de produção de biofilme uma terapia alternativa eficaz para a quebra desse biofilme associado a um tratamento antibacteriano específico. A correlação desses testes possivelmente fornecerá subsídios ainda mais importantes para melhorar a eficiência reprodutiva dos rebanhos equinos.


Endometritis is the main cause of infertility in mares, in addition to promote major economic and productive disorders. The objective of this work was to carry out a systematic review of equine endometritis, its etiology, mechanical and chemotactic uterine defense mechanisms. Also evaluating the main microorganisms involved in the process as well as their virulence and antimicrobial resistance. We also present a proposal for a diagnostic approach contemplating the identification of agents, pharmacological susceptibility associated with presence of biofilm production, as well as its graduation, thus enabling, in case of biofilm present, an effective alternative therapy to break this biofilm associated with an specific antibacterial treatment. The correlation of these tests will possibly provide even more important subsidies to improve reproductive efficiency of equine herds.


Assuntos
Animais , Anti-Infecciosos/análise , Biofilmes , Endometrite/microbiologia , Endometrite/veterinária
20.
Vet. Not. (Online) ; 27(3): 1-23, 1 out. 2021. ilus
Artigo em Português | VETINDEX | ID: biblio-1502548

Resumo

O transplante de espermatogônias tronco (SSCs, do inglês Spermatogonial Stem Cell) é uma biotecnologia que consiste na transferência de células tronco testiculares de um doador fértil para um receptor cuja espermatogênese endógena foi suprimida. Essa técnica pode ser aplicada para a produção de machos que gerem uma progênie com características genotípicas do doador selecionado. Especialmente na bovinocultura, tanto de leite como de corte, o transplante de SSCs tem o potencial de substituir a inseminação artificial (IA). Pode-se também, colocar SSCs de um mesmo doador (de genética superior) em mais de um receptor o que aumentaria o número de filhos desse doador. Além disso, possui outras aplicações como a restauração da fertilidade em homens após o tratamento de câncer, conservação de espécies ameaçadas de extinção e tratamento de causas específicas de infertilidade. Assim, com esta revisão tem-se como propósito discorrer acerca de uma biotecnologia da reprodução que permitirá a propagação do valor genético de doadores de sêmen considerados de alto valor zootécnico.


Spermatogonial Stem Cell (SSCs) transplantation is a biotechnology that consists in the transfer of testicular stem cells from a fertile donor to a recipient whose endogenous spermatogenesis has been depleted. This technique can be applied to the production of males that generate a progeny with genotypic characteristics of the selected donor. Especially in beef and dairy cattle, SSCs transplantation has the potential to replace artificial insemination (AI). In addition, it has other applications such as restoring fertility in human species after cancer treatment, conserving endangered species and treating specific causes of infertility. Thus, this aim of this review is to discuss the perspectives of reproductive biotechnology that allows the propagation of the genetic material of high pedigree males.


Assuntos
Masculino , Animais , Bioengenharia/história , Espermatogônias , Transplante de Células-Tronco/veterinária , Biotecnologia , Inseminação Artificial/tendências , Inseminação Artificial/veterinária
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