Morphological aspects and germ cells of male reproductive tract of river stingray, Potamotrygon amandae

The family Potamotrygonidae are the only species of stingrays restricted to fresh water and located exclusively in South America. The objective of this research was to analyze the morphological aspects and germ cells of the male reproductive tract of Potamotrygon amandae. The samples were fixed in 10% formalin, and then dehydrated in an ascending ethanol series (70 to 100%). To carry out light microscopy analyses, they were embedded in paraffin, cut and stained; as for scanning electron microscopy analyses, the samples were dried, glued in metallic bases and metalized. The gross morphology consisted of the following paired organs: testis, epididymis, deferent duct, Leydig gland, seminal vesicle, clasper, and the clasper gland. Microscopically, several stages of spermatogenesis were observed in the testis, occurring in spherical follicles, similar to other stingrays. The epididymis was formed by one duct subdivided in various tubules. The deferent ducts were continuous with the epididymis, and the lumen was full of spermatozoa. The Leydig glands consisted of glandular units with eosinophilic content in the lumen of some, and the deferent ducts ran parallel to the ventral portion. The seminal vesicles possessed numerous compartments to store the sperm, with a wall similar to a hive, and the lumen was full of spermatozoa. Alcian Blue (AB) and Periodic Schiff-Acid (PAS) performed in the Leydig Gland, deferens ducts and seminal vesicle was positive only in the connective tissue, the cilia were PAS+ and the nuclei stained weakly for AB. The clasper gland was composed of unit glands and was covered with striated muscle externally. It stained very well with Periodic Schiff-Acid. The morphological aspects of the male reproductive tract of Potamotrygon amandae were similar to other stingrays.(AU)

Bilateral testicular tumors in a non-cryptorchid dog: coexistence of Sertoli cell and interstitial endocrine cell tumors

Background: Testicular neoplasms in dogs are more frequent than in other animal species, representing the most common tumors in elderly subjects after skin neoplasms. In cryptorchid subjects, the risk of neoplastic degeneration is high. The cytological examination is essential to differentiate the type of neoplasia and to determinate the best diagnostic approach. Aim of this report was to describe clinical and histopathological features of a dog with coexistence of Sertoli cell and interstitial endocrine cell tumors in a non-cryptorchid dog. Case: A 9-year-old non-neutered male dog, German Spitz breed, was presented to the veterinary clinic. On clinical examination, the dog had a body condition (BCS 6/9), pink mucous membranes, capillary refill time (CRT) < 2 sec, lymph nodes of normal size, afebrile, normal heart and respiratory rate. The abdomen was depressible to palpation, without pain, the skin appeared hyperpigmented, with generalized presence of comedones, pendular foreskin and absence of hair at the abdominal level, in the ventral portion of the trunk and neck, scant hair also at the level of the inner thighs and in perianal. At ultrasounds examinations, the right testicle presented a hypoechoic circular focal lesion, in the caudal pole, of 0.7 cm in diameter, well defined, echogenicity and a remnant of normal echostructure, smooth and regular margins; the left testicle showed an increase in size, irregular margins, with a heterogeneous echo structure, given by hypoechoic areas, referred to cysts, hemorrhagic or necrotic areas. The hemogram reported slightly microcytic and normochromic regenerative anemia. The leukogram showed monocytosis. The absence of the typical stress leukogram characterized by neutrophilia, lymphopenia and eosinopenia, and the reduction of ALP allows to rule out Cushing's disease. In order to rule out hypothyroidism due to the inhibitory effect of estrogens on the release of thyroid-stimulating hormone (TSH) the concentration of total thyroxine was analyzed, reporting normal values excluding hypothyroidism. Blood oestradiol 17-ß (E2) concentration was increased, with a normal testosterone (T) concentration of 0.30 ng/mL. Given the suspicion of the presence of testicular tumors, castration was performed by the surgical excision of both gonads, after ruling out the presence of abdominal or pulmonary metastases by chest and abdominal radiography. Discussion: The clinical, histopathological findings supported the diagnosis of testicular tumors. According to the pathological report, both gonads presented parenchymal nodular neoplastic nodular areas referring to the Sertoli sustentacular cells in the right testicle, to the proliferation of Leydig interstitial cells in the left one. The nodule in the left testicle was unencapsulated and showed a solid-diffuse pattern. Neoplastic cells were irregular polygonal, medium to large in size with moderate nucleus/cytoplasm ratio and moderate anisocytosis. In the right testicle, the nodule was heterogeneous in consistency and a diffuse pattern was present. Neoplastic cells were polygonal morphology, had a moderate nucleus/cytoplasm ratio and were organized tubules lined and obliterated the extensive cell growth. Bilateral orchiectomy allowed to improve the clinical signs, and 3 months after surgery, the animal was in good health, with evident improvement skin lesions. The E2 analysis was repeated, detecting normal values, demonstrating that testicular neoplasm in this patient were involved in E2 production; also T concentration decreased considerably from 0.30 to < 0.07 values.

Intertubular morphometric and ultrastructural testes analyses in mdx mice

Duchenne Muscular Dystrophy (DMD) reproductive alterations and the influence of antioxidant treatments may aid in understanding morphometry testicular quantification. In this context, the aim of the present study was to characterize the intertubular compartment (ITC) morphometry of animal testes in mdx mice supplemented with ascorbic acid (AA). Sixteen mice were used, namely the C57BL/10 (non-dystrophic) and C57BL/10Mdx (dystrophic) lineages, distributed into the following groups: Control (C60), Dystrophic (D60), Control supplemented with AA (CS60), Dystrophic supplemented with AA (DS60). A total of 200 mg/kg of AA were administered to mice for 30 days. Subsequently, the testicles were collected, weighed, and fragmented. The obtained fragments were fixed in Karnovsky's solution (pH 7.2) and embedded in historesin for morphometric and transmission electron microscopy assessments. Leydig cells were hypertrophic in the D60 group, but was reverted by AA supplementation in the DS60 group. The DS60 group also exhibited increased intertubular volume compared to the CS60 group. The ultrastructural images identified multilamellar bodies in dystrophic animals (lipid storage) and telocyte cells (transport substances) in both control and dystrophic animals. Morphometric alterations were, therefore, noted in the intertubular compartment due to Duchenne muscular dystrophy (DMD), with AA administration capable of altering Leydig cells in this condition.(AU)

Diagnóstico e tratamento videoassistido de tumor de células de Leydig em cadela com síndrome do ovário remanescente / Videoassisted diagnosis and treatment for Leydig cell tumor in a bitch with remaining ovary syndrome

Neoplasias ovarianas de origem primária são incomuns em cadelas, exigindo métodos diagnósticos e terapêuticos mais precisos. Fêmeas também possuem células intersticiais secretoras de esteróides, contudo o aparecimento do tumor de células de Leydig é considerado raro. Uma cadela sem raça definida e 12 anos apresentava sinais de estro após ovariohisterectomia pré-púbere. Na avaliação ultrassonográfica, foi identificada uma massa localizada na região abdominal lateral direita, sugestiva de ovário remanescente. Durante a inspeção laparoscópica foi identificada a massa distante caudalmente do coto ovariano direito, inserida em região de mesentério jejunal. O segmento intestinal foi apreendido e exteriorizado de maneira videoassistida, optando-se pela remoção do tumor associado à enterectomia do segmento afetado. A avaliação do coto ovariano esquerdo, revelou um cisto de 0,5cm de diâmetro que também foi removido. A análise histopatológica foi sugestiva de neoplasia de células intersticiais (tumor de células de Leydig) e um cisto epitelial ovariano. O tumor de células de Leydig é relatado com freqüência em machos, entretanto, sua aparição em fêmeas é considerada extremamente incomum acometendo principalmente animais não esterilizados. A neoplasia não aderida ao coto ovariano comprova ainda que resquícios de tecido ovariano que porventura fiquem livres na cavidade abdominal podem ser responsáveis pelo desenvolvimento da síndrome do ovário remanescente e neoplasias. O prognóstico para neoplasias ovarianas sem metástases é favorável, principalmente quando aplicada a intervenção cirúrgica como método terapêutico. O uso da videocirurgia para auxiliar no diagnóstico e tratamento demonstrou-se adequado para o caso descrito, mas que deve ser complementado pela análise histopatológica.

Primary ovarian neoplasms are uncommon in bitches, requiring more accurate diagnostic and therapeutic methods. Females also have steroid-secreting interstitial cells, however, the appearance of the Leydig cell tumor is considered rare. A 12-year-old mongrel bitch showed signs of estrus after prepubertal ovariohysterectomy. Ultrasound evaluation located a mass in the right lateral abdominal region, suggestive of a remaining ovary. During laparoscopic exploration, the mass was identified caudally distant from the right ovarian stump, adhered to the jejunal mesentery. The intestinal segment was apprehended and exteriorized by video-assisted technique, and removal of the tumor was performed associated with enterectomy of the affected segment. Evaluation of the left ovarian stump revealed a 0.5cm diameter cyst that was also removed. Histopathological analysis was suggestive of interstitial cell neoplasia (Leydig cell tumor) and an ovarian epithelial cyst. The Leydig cell tumor is frequently related in males, however, its appearance in bitches is considered uncommon, affecting mainly nonsterile animals. The mass non-adhered to the ovarian stump proves that remnants of ovarian tissue that may be free in the abdominal cavity and may be responsible for the development of the remaining ovary syndrome and neoplasms. The prognosis for ovarian neoplasms without metastasis is favorable, especially when surgical intervention is applied as a therapeutic method. The use of video surgery to assist in the diagnosis and treatment was considered appropriate for the case described, but it must be complemented by histopathological analysis.

Testicular tumors in 190 dogs: clinical, macroscopic and histopathological aspects / Neoplasmas testiculares em 190 cães: aspectos clínicos, macroscópicos e histopatológicos

This study aimed to characterize the prevalence and clinical, macroscopic and histopathological aspects of dogs affected by testicular tumors based on biopsy specimens from the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria (LPV-UFSM) over 19 years. Parameters regarding the age, size, and breed of the affected dogs were also established. Of all dogs with some type of neoplasm submitted to histopathological analysis at the LPV over these 19 years (n=1,900), 213 (11.2%) had at least one testicular neoplasm. The tissues of 190 dogs (with 220 neoplasms) were available for histological reassessment. The dogs in this study had different types of testicular tumors with relatively similar frequencies. In descending order, the most frequent testicular neoplasms were seminomas (88/220), Leydig (interstitial) cell tumor (LCT; 64/220), Sertoli cell tumor (SCT; 61/220), and mixed germ cell-sex cord stromal tumor (MGSCT) (07/220). Among the dogs of defined breed (119 cases), large breeds had the largest number of cases (50/119), followed by small (47/119) and medium-sized (22/119) breeds. The ages of dogs affected by testicular tumors ranged from 10 months to 18 years. Increased testicular volume was the most common clinical manifestation. Eleven dogs presented information about clinical signs suggestive of hyperestrogenism syndrome (feminization). In seminomas, the diffuse pattern predominated over the intratubular pattern. Two sites (luminal and basal compartments) suggestive of the onset of neoplastic transformations in germ cells were observed in intratubular seminomas. They corroborate the hypothesis that canine seminomas possibly have pathogenesis similar to that observed in human spermatocytic seminomas. The SCTs and LCTs presented high cell morphology variation. SCTs had neoplastic cells organized in five different histological arrangements. As for LCT, solid-diffuse and cystic-vascular histological patterns were the most commonly observed. Through this study, it was possible to establish some of the leading clinical, macroscopic, and histopathological aspects of testicular neoplasms diagnosed over 19 years in the area covered by the LPV-UFSM.(AU)

Este estudo teve por objetivo caracterizar a prevalência, aspectos clínicos, macroscópicos e histopatológicos dos cães acometidos por neoplasmas testiculares, a partir dos espécimes de biópsias do Laboratório de Patologia Veterinária da Universidade Federal de Santa Maria (LPV-UFSM) em 19 anos. Parâmetros quanto à idade, porte, raça dos cães acometidos também foram estabelecidos. De todos os cães com algum tipo de neoplasma submetido à análise histopatológica no LPV nesses 19 anos (n=1.900), 213 (11,2%) tinham ao menos um neoplasma testicular. Os tecidos de 190 cães (com 220 neoplasmas) estavam disponíveis para reavaliação histológica. Os cães deste estudo apresentaram diferentes tipos de neoplasmas testiculares com frequências relativamente semelhantes. Em ordem decrescente, os neoplasmas testiculares mais frequentes foram: seminomas (88/220), leydigomas (64/220), sertoliomas (61/220) e o tumor misto de células germinativas e do estroma do cordão sexual (MGSCT; 07/220). Dentre os cães com raça definida (119 casos), as raças de grande porte tiveram o maior número de casos (50/119), seguido das raças de pequeno (47/119) e médio porte (22/119). As idades dos cães acometidos por neoplasmas testiculares variaram de 10 meses a 18 anos. Aumento de volume testicular foi a manifestação clínica mais comum. Onze cães tinham informações sobre sinais clínicos sugestivos da síndrome da feminilização. Nos seminomas, houve o predomínio do padrão difuso sobre o intratubular. Dois locais (compartimentos luminal e basal) sugestivos de início das transformações neoplásicas nas células germinativas foram observados nos seminomas intratubulares, corroborando com a hipótese de que os seminomas caninos possivelmente tem patogênese semelhante à observada nos seminomas espermatocíticos humanos. Sertoliomas e leydigomas foram neoplasmas com alta variação na morfologia celular. Os sertoliomas tinham células neoplásicas dispostas em cinco arranjos histológicos distintos. Quanto aos leydigomas, os padrões histológicos sólido-difuso e cístico-vascular foram os mais comumente observados. Através deste estudo foi possível estabelecer alguns dos principais aspectos clínicos, macroscópicos e histopatológicos dos neoplasmas testiculares diagnosticados em 19 anos na área de abrangência do LPV-UFSM.(AU)

Testicular tumors in 190 dogs: clinical, macroscopic and histopathological aspects

ABSTRACT: This study aimed to characterize the prevalence and clinical, macroscopic and histopathological aspects of dogs affected by testicular tumors based on biopsy specimens from the Laboratório de Patologia Veterinária of the Universidade Federal de Santa Maria (LPV-UFSM) over 19 years. Parameters regarding the age, size, and breed of the affected dogs were also established. Of all dogs with some type of neoplasm submitted to histopathological analysis at the LPV over these 19 years (n=1,900), 213 (11.2%) had at least one testicular neoplasm. The tissues of 190 dogs (with 220 neoplasms) were available for histological reassessment. The dogs in this study had different types of testicular tumors with relatively similar frequencies. In descending order, the most frequent testicular neoplasms were seminomas (88/220), Leydig (interstitial) cell tumor (LCT; 64/220), Sertoli cell tumor (SCT; 61/220), and mixed germ cell-sex cord stromal tumor (MGSCT) (07/220). Among the dogs of defined breed (119 cases), large breeds had the largest number of cases (50/119), followed by small (47/119) and medium-sized (22/119) breeds. The ages of dogs affected by testicular tumors ranged from 10 months to 18 years. Increased testicular volume was the most common clinical manifestation. Eleven dogs presented information about clinical signs suggestive of hyperestrogenism syndrome (feminization). In seminomas, the diffuse pattern predominated over the intratubular pattern. Two sites (luminal and basal compartments) suggestive of the onset of neoplastic transformations in germ cells were observed in intratubular seminomas. They corroborate the hypothesis that canine seminomas possibly have pathogenesis similar to that observed in human spermatocytic seminomas. The SCTs and LCTs presented high cell morphology variation. SCTs had neoplastic cells organized in five different histological arrangements. As for LCT, solid-diffuse and cystic-vascular histological patterns were the most commonly observed. Through this study, it was possible to establish some of the leading clinical, macroscopic, and histopathological aspects of testicular neoplasms diagnosed over 19 years in the area covered by the LPV-UFSM.

RESUMO: Este estudo teve por objetivo caracterizar a prevalência, aspectos clínicos, macroscópicos e histopatológicos dos cães acometidos por neoplasmas testiculares, a partir dos espécimes de biópsias do Laboratório de Patologia Veterinária da Universidade Federal de Santa Maria (LPV-UFSM) em 19 anos. Parâmetros quanto à idade, porte, raça dos cães acometidos também foram estabelecidos. De todos os cães com algum tipo de neoplasma submetido à análise histopatológica no LPV nesses 19 anos (n=1.900), 213 (11,2%) tinham ao menos um neoplasma testicular. Os tecidos de 190 cães (com 220 neoplasmas) estavam disponíveis para reavaliação histológica. Os cães deste estudo apresentaram diferentes tipos de neoplasmas testiculares com frequências relativamente semelhantes. Em ordem decrescente, os neoplasmas testiculares mais frequentes foram: seminomas (88/220), leydigomas (64/220), sertoliomas (61/220) e o tumor misto de células germinativas e do estroma do cordão sexual (MGSCT; 07/220). Dentre os cães com raça definida (119 casos), as raças de grande porte tiveram o maior número de casos (50/119), seguido das raças de pequeno (47/119) e médio porte (22/119). As idades dos cães acometidos por neoplasmas testiculares variaram de 10 meses a 18 anos. Aumento de volume testicular foi a manifestação clínica mais comum. Onze cães tinham informações sobre sinais clínicos sugestivos da síndrome da feminilização. Nos seminomas, houve o predomínio do padrão difuso sobre o intratubular. Dois locais (compartimentos luminal e basal) sugestivos de início das transformações neoplásicas nas células germinativas foram observados nos seminomas intratubulares, corroborando com a hipótese de que os seminomas caninos possivelmente tem patogênese semelhante à observada nos seminomas espermatocíticos humanos. Sertoliomas e leydigomas foram neoplasmas com alta variação na morfologia celular. Os sertoliomas tinham células neoplásicas dispostas em cinco arranjos histológicos distintos. Quanto aos leydigomas, os padrões histológicos sólido-difuso e cístico-vascular foram os mais comumente observados. Através deste estudo foi possível estabelecer alguns dos principais aspectos clínicos, macroscópicos e histopatológicos dos neoplasmas testiculares diagnosticados em 19 anos na área de abrangência do LPV-UFSM.

Leydigocitoma canino: aspectos ultrassonográficos, citológicos e histopatológicos / Canine Leydigocitoma: ultrasonographic, cytological and histopathological aspects

In non-neutered dogs, the testis is the second most common site for development of neoplasms, frequently occurring in elderly animals and with orchiectomy as main treatment. Regarding the great relevance of testicular disorders and the importance of accurate diagnoses, this study aims to report a case of canine leydigocytoma at the Veterinary Hospital in the State University of Ceará. The 10-years-old non-neutered Pitbull-breed animal was attended presenting hematuria and, physical examination showed a bilateral nodular formation in testicles in addition to an enlarged prostate. In ultrasonographic examination, the prostate was enlarged, presenting irregular shape and heterogeneous echotexture. These changes indicate the presence of prostatic hyperplasia. Both testicles presented well-defined hypoechogenic regions with evident marginal and internal vascularization, which indicate neoplastic neoformation. In cytology, a hypercellular epithelial sample was observed with cellular modifications suggestive of interstitial cell neoplasia. In the lab, macroscopically, the testis showed multiple soft, well-defined, white-colored to brownish nodules. Histopathology revealed neoplastic proliferation of Leydig cells, sustained by moderate fibrovascular stroma, with degenerated adjacent parenchyma and without signs of malignancy. Four months after orchiectomy, the prostate turned to normal dimensions, with no further ultrasonographic changes. This case reinforces the importance of bilateral orchiectomy and ultrasound for prostatic abnormalities and testicular neoplasms. The association of these conditions is not commonly described in the literature, being an important contribution in this regard.

Leydigocitoma canino: aspectos ultrassonográficos, citológicos e histopatológicos / Canine Leydigocitoma: ultrasonographic, cytological and histopathological aspects

In non-neutered dogs, the testis is the second most common site for development of neoplasms, frequently occurring in elderly animals and with orchiectomy as main treatment. Regarding the great relevance of testicular disorders and the importance of accurate diagnoses, this study aims to report a case of canine leydigocytoma at the Veterinary Hospital in the State University of Ceará. The 10-years-old non-neutered Pitbull-breed animal was attended presenting hematuria and, physical examination showed a bilateral nodular formation in testicles in addition to an enlarged prostate. In ultrasonographic examination, the prostate was enlarged, presenting irregular shape and heterogeneous echotexture. These changes indicate the presence of prostatic hyperplasia. Both testicles presented well-defined hypoechogenic regions with evident marginal and internal vascularization, which indicate neoplastic neoformation. In cytology, a hypercellular epithelial sample was observed with cellular modifications suggestive of interstitial cell neoplasia. In the lab, macroscopically, the testis showed multiple soft, well-defined, white-colored to brownish nodules. Histopathology revealed neoplastic proliferation of Leydig cells, sustained by moderate fibrovascular stroma, with degenerated adjacent parenchyma and without signs of malignancy. Four months after orchiectomy, the prostate turned to normal dimensions, with no further ultrasonographic changes. This case reinforces the importance of bilateral orchiectomy and ultrasound for prostatic abnormalities and testicular neoplasms. The association of these conditions is not commonly described in the literature, being an important contribution in this regard.(AU)

Effect of bacterial endotoxin lipopolysaccharide treatment on duck Leydig cells

This study aimed to investigate the effects of bacterial endotoxin lipopolysaccharide (LPS) on hormone production and gene expression in duck Leydig cells and its underlying mechanisms. Leydig cells were collected from 200-day-old mallard ducks and divided into five treatment groups (0, 50, 100, 200, and 400 ng/mL LPS). After treatment with LPS for 6, 12, 24, and 48 h, testosterone, activin, and inhibin levels in the cell supernatants were determined using enzyme-linked immunosorbent assay (ELISA) kits. The expression levels of testosterone synthesis-related genes, including steroidogenic acute regulatory protein (StAR), 3-beta-hydroxysteroid dehydrogenase (3β-HSD), and cytochrome P450 aromatase (P450arom), and reproductive-related genes, including gonadotropin-inhibitory hormone receptor (GnIHR), follicle stimulating hormone receptor (FSHR), and luteinizing hormone receptor (LHR) were detected using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully isolated and cultured duck Leydig cells with cell purity above 90%. Compared with the control group, the levels of testosterone, activin, and inhibin secreted in Leydig cells decreased gradually with increasing LPS concentration. After treatment with LPS, the expression of StAR and 3β-HSD genes in Leydig cells was upregulated at 12 h, and that of GnIHR was upregulated at 24 h; whereas the expression of FSHR and LHR was reduced at 24 h. This study indicates that LPS can inhibit the secretion of hormones and regulate the expression of related genes in duck Leydig cells.(AU)

Effect of bacterial endotoxin lipopolysaccharide treatment on duck Leydig cells

This study aimed to investigate the effects of bacterial endotoxin lipopolysaccharide (LPS) on hormone production and gene expression in duck Leydig cells and its underlying mechanisms. Leydig cells were collected from 200-day-old mallard ducks and divided into five treatment groups (0, 50, 100, 200, and 400 ng/mL LPS). After treatment with LPS for 6, 12, 24, and 48 h, testosterone, activin, and inhibin levels in the cell supernatants were determined using enzyme-linked immunosorbent assay (ELISA) kits. The expression levels of testosterone synthesis-related genes, including steroidogenic acute regulatory protein (StAR), 3-beta-hydroxysteroid dehydrogenase (3β-HSD), and cytochrome P450 aromatase (P450arom), and reproductive-related genes, including gonadotropin-inhibitory hormone receptor (GnIHR), follicle stimulating hormone receptor (FSHR), and luteinizing hormone receptor (LHR) were detected using quantitative real-time polymerase chain reaction (qRT-PCR). We successfully isolated and cultured duck Leydig cells with cell purity above 90%. Compared with the control group, the levels of testosterone, activin, and inhibin secreted in Leydig cells decreased gradually with increasing LPS concentration. After treatment with LPS, the expression of StAR and 3β-HSD genes in Leydig cells was upregulated at 12 h, and that of GnIHR was upregulated at 24 h; whereas the expression of FSHR and LHR was reduced at 24 h. This study indicates that LPS can inhibit the secretion of hormones and regulate the expression of related genes in duck Leydig cells.

Testicular interstitial cell tumor with disseminated cutaneous metastasis in a dog

Background: Although testicular tumors commonly develop in the genital tract of dogs, only few studies have reportedabout metastatic testicular tumors in dogs to date. Therefore, the present study aimed to describe the gross, histopathological, and immunohistochemical findings in a dog with metastatic interstitial cell tumor.Case: A 6-year-old male Beagle underwent clinical examination due to enlargement of the left testicle and nodules inthe skin of the left inguinal region and of the scrotum. The left testicle was cryptorchid (inguinal), and the right testiclewas intrascrotal. Both testicles and affected skin fragments were surgically removed. The right testicle was normal insize and did not exhibit gross changes. However, the left testicular parenchyma had been completely replaced by a firm,grayish-to-white neoplastic mass with an irregular surface. The inguinal and scrotal skin fragments were thickened by theneoplastic masses. Histologically, the left testicular parenchyma was completely obliterated by a neoplastic mass consistingof round-to-oval and polygonal cells. The neoplastic cells generally had abundant eosinophilic cytoplasm with indistinctcell limits. The nuclei were round and small, with aggregated chromatin. However, some cells exhibited high degrees ofatypia and pleomorphism and large nuclei with granular chromatin. Mitotic figures were observed at high frequenciesof up to 11 per high-power field (400×). The mass in the left testicle was diagnosed as malignant interstitial cell tumor.The sections of the inguinal skin and scrotum presented with neoplastic cells similar to those observed in the left testicle,which is consistent with the metastasis of the malignant testicular interstitial cell tumor. On the basis of necropsy, multiple cutaneous and subcutaneous nodules were randomly distributed throughout the body, primarily in the prepuce, head,and limbs. Histological evaluations of the skin nodules...

Testicular interstitial cell tumor with disseminated cutaneous metastasis in a dog

Background: Although testicular tumors commonly develop in the genital tract of dogs, only few studies have reportedabout metastatic testicular tumors in dogs to date. Therefore, the present study aimed to describe the gross, histopathological, and immunohistochemical findings in a dog with metastatic interstitial cell tumor.Case: A 6-year-old male Beagle underwent clinical examination due to enlargement of the left testicle and nodules inthe skin of the left inguinal region and of the scrotum. The left testicle was cryptorchid (inguinal), and the right testiclewas intrascrotal. Both testicles and affected skin fragments were surgically removed. The right testicle was normal insize and did not exhibit gross changes. However, the left testicular parenchyma had been completely replaced by a firm,grayish-to-white neoplastic mass with an irregular surface. The inguinal and scrotal skin fragments were thickened by theneoplastic masses. Histologically, the left testicular parenchyma was completely obliterated by a neoplastic mass consistingof round-to-oval and polygonal cells. The neoplastic cells generally had abundant eosinophilic cytoplasm with indistinctcell limits. The nuclei were round and small, with aggregated chromatin. However, some cells exhibited high degrees ofatypia and pleomorphism and large nuclei with granular chromatin. Mitotic figures were observed at high frequenciesof up to 11 per high-power field (400×). The mass in the left testicle was diagnosed as malignant interstitial cell tumor.The sections of the inguinal skin and scrotum presented with neoplastic cells similar to those observed in the left testicle,which is consistent with the metastasis of the malignant testicular interstitial cell tumor. On the basis of necropsy, multiple cutaneous and subcutaneous nodules were randomly distributed throughout the body, primarily in the prepuce, head,and limbs. Histological evaluations of the skin nodules...(AU)

Testicular teratoma in a unilateral right-sided abdominal cryptorchid horse

Background: Cryptorchidism is characterized by the incomplete descent of one or both testicles to the scrotum, beinga hereditary alteration and frequently an unilateral condition. Besides the sexual and aggressive behaviour, the retainedtesticle is commonly located in abdominal cavity, being considered a risk factor for neoplasm development. The mostcommon testicular neoplasm reported in mammalian species are Sertoli cell tumors, Leydig cell tumors, seminomas andteratomas. A presumptive diagnosis of testicular tumor can be achieved by ultrasonography, although the definitive diagnosis is obtained only by histopathology. In this report, we are presenting a case of testicular teratoma in an unilateralabdominal cryptorchid horse.Case: A 3 year-old stallion, American Quarter Horse, was attended and presented a right testicle retained inside the abdomen and a left testicle in the scrotum. Transrectal palpation was used to identify a round and firm structure, presumablythe right testicle, lateral to the urinary bladder and located inside abdomen. Further, a transrectal ultrasound examinationshowed a complex, round mass with irregular edges containing both cystic and solid structures, hypoechoic fluid-filledcavities separated by linear hyperechoic septa. After a clinical examination, the animal was diagnosed with cryptorchidismand was submitted to orchiectomy and cryptorchidectomy by inguinal approach. Surgery was performed under generalanesthesia and postoperative care included cold shower, anti-inflammatory and antibiotic therapy. Testicles were surgicallyremoved and further sent for histopathological examination. The visual appearance...

Testicular teratoma in a unilateral right-sided abdominal cryptorchid horse

Background: Cryptorchidism is characterized by the incomplete descent of one or both testicles to the scrotum, beinga hereditary alteration and frequently an unilateral condition. Besides the sexual and aggressive behaviour, the retainedtesticle is commonly located in abdominal cavity, being considered a risk factor for neoplasm development. The mostcommon testicular neoplasm reported in mammalian species are Sertoli cell tumors, Leydig cell tumors, seminomas andteratomas. A presumptive diagnosis of testicular tumor can be achieved by ultrasonography, although the definitive diagnosis is obtained only by histopathology. In this report, we are presenting a case of testicular teratoma in an unilateralabdominal cryptorchid horse.Case: A 3 year-old stallion, American Quarter Horse, was attended and presented a right testicle retained inside the abdomen and a left testicle in the scrotum. Transrectal palpation was used to identify a round and firm structure, presumablythe right testicle, lateral to the urinary bladder and located inside abdomen. Further, a transrectal ultrasound examinationshowed a complex, round mass with irregular edges containing both cystic and solid structures, hypoechoic fluid-filledcavities separated by linear hyperechoic septa. After a clinical examination, the animal was diagnosed with cryptorchidismand was submitted to orchiectomy and cryptorchidectomy by inguinal approach. Surgery was performed under generalanesthesia and postoperative care included cold shower, anti-inflammatory and antibiotic therapy. Testicles were surgicallyremoved and further sent for histopathological examination. The visual appearance...(AU)

Estágio reprodutivo, histologia e morfometria sazonal do testículo de Dermanura cinerea (Chiroptera: Phyllostomidae) em fragmento de Mata Atlântica no Litoral Sul de Pernambuco, Brasil / Reproductive stage, histology and seasonal morphometry of the testes of Dermanura cinerea (Chiroptera: Phyllostomidae) in Atlantic Forest fragment on the South Coast of Pernambuco, Brazil

This study analyzed the reproductive stage, histology and morphometry of the testes of Dermanura cinerea (Gervais, 1856) in Atlantic Forest fragment on the South Coast of Pernambuco. Specimens were captured by mist net, and collections occurred monthly over two consecutive nights for 18 months. The meteorological data were grouped in rainy and dry months. Eighteen adult specimens were used, which were classified in males with descendant testes and with testes no descendant. For the histological analysis, the testes were collected, fixed and processed following the routine histological technique. The slides obtained were stained by Hematoxylin-Eosin. In the morphometric analysis the area of occupation of the tubular and intertubular compartment were measured, the number of Leydig, Sertoli, spermatocytes and elongated spermatids were quantified. The morphometric data were submitted to statistical analysis. Dermanura cinerea presented higher sperm production activity in dry months and higher hormone production in rainy months. This information is also related to the reproductive pattern of the females and to the increase in rainfall indexes, since it is a determinant factor in the mediation of the food availability of the frugivorous species, such as D. cinerea in the Atlantic Forest area of Pernambuco.(AU)

Esse estudo analisou o estágio reprodutivo, a histologia e a morfometria dos testículos de Dermanura cinerea (Gervais, 1856) em fragmento de Mata Atlântica no Litoral Sul de Pernambuco. Os espécimes foram capturados por redes de neblina e as coletas ocorreram mensalmente, ao longo de duas noites consecutivas, durante 18 meses. Os dados meteorológicos foram agrupados em meses chuvosos e secos. Foram utilizados 18 espécimes adultos, os quais foram classificados em machos com testículos descendentes e não descendentes. Para as análises histológicas, os testículos foram coletados, fixados e processados seguindo a técnica histológica de rotina. As lâminas obtidas foram coradas por Hematoxilina-Eosina. Nas análises morfométricas foram mensuradas a área de ocupação do compartimento tubular e intertubular, quantificados o número de células de Leydig, de Sertoli, de espermatócitos e de espermátides alongadas. Os dados morfométricos foram submetidos às análises estatísticas. Dermanura cinerea apresentou maior atividade de produção espermática em meses secos e maior produção hormonal em meses chuvosos. Essas informações estão relacionadas também com o padrão reprodutivo das fêmeas e com a elevação nos índices pluviométricos, já que é um fator determinante na mediação da disponibilidade alimentar das espécies frugívoras, como D. cinerea em área de Mata Atlântica de Pernambuco.(AU)

Estágio reprodutivo, histologia e morfometria sazonal do testículo de Dermanura cinerea (Chiroptera: Phyllostomidae) em fragmento de Mata Atlântica no Litoral Sul de Pernambuco, Brasil / Reproductive stage, histology and seasonal morphometry of the testes of Dermanura cinerea (Chiroptera: Phyllostomidae) in Atlantic Forest fragment on the South Coast of Pernambuco, Brazil

This study analyzed the reproductive stage, histology and morphometry of the testes of Dermanura cinerea (Gervais, 1856) in Atlantic Forest fragment on the South Coast of Pernambuco. Specimens were captured by mist net, and collections occurred monthly over two consecutive nights for 18 months. The meteorological data were grouped in rainy and dry months. Eighteen adult specimens were used, which were classified in males with descendant testes and with testes no descendant. For the histological analysis, the testes were collected, fixed and processed following the routine histological technique. The slides obtained were stained by Hematoxylin-Eosin. In the morphometric analysis the area of occupation of the tubular and intertubular compartment were measured, the number of Leydig, Sertoli, spermatocytes and elongated spermatids were quantified. The morphometric data were submitted to statistical analysis. Dermanura cinerea presented higher sperm production activity in dry months and higher hormone production in rainy months. This information is also related to the reproductive pattern of the females and to the increase in rainfall indexes, since it is a determinant factor in the mediation of the food availability of the frugivorous species, such as D. cinerea in the Atlantic Forest area of Pernambuco.

Esse estudo analisou o estágio reprodutivo, a histologia e a morfometria dos testículos de Dermanura cinerea (Gervais, 1856) em fragmento de Mata Atlântica no Litoral Sul de Pernambuco. Os espécimes foram capturados por redes de neblina e as coletas ocorreram mensalmente, ao longo de duas noites consecutivas, durante 18 meses. Os dados meteorológicos foram agrupados em meses chuvosos e secos. Foram utilizados 18 espécimes adultos, os quais foram classificados em machos com testículos descendentes e não descendentes. Para as análises histológicas, os testículos foram coletados, fixados e processados seguindo a técnica histológica de rotina. As lâminas obtidas foram coradas por Hematoxilina-Eosina. Nas análises morfométricas foram mensuradas a área de ocupação do compartimento tubular e intertubular, quantificados o número de células de Leydig, de Sertoli, de espermatócitos e de espermátides alongadas. Os dados morfométricos foram submetidos às análises estatísticas. Dermanura cinerea apresentou maior atividade de produção espermática em meses secos e maior produção hormonal em meses chuvosos. Essas informações estão relacionadas também com o padrão reprodutivo das fêmeas e com a elevação nos índices pluviométricos, já que é um fator determinante na mediação da disponibilidade alimentar das espécies frugívoras, como D. cinerea em área de Mata Atlântica de Pernambuco.

Ipomoea brasiliana poisoning on buck reproduction / Efeitos da intoxicação por Ipomoea brasiliana na reprodução de caprinos

Swainsonine-containing plants comprise a group of important poisonous plants in Brazil. This research aimed to characterize both the behavioral changes related to reproduction and appearance of lesions in the reproductive system of bucks poisoned by Ipomoea brasiliana. I. brasiliana plants were collected and administered at a dose of 4g/kg (800µg swainsonine/kg) to two groups of bucks for 45 days. Goats from Group I were euthanized on the 46th day of the experiment, and goats from Group II were euthanized on the 120th day. Group III was composed of goats that did not receive I. brasiliana and were euthanized on the 120th day of the experiment. Reproductive behavioral changes were observed starting on day 20 and were characterized by an absence of courtship behavior, and Flehmen reflex, decrease or loss of libido and inability to perform mating. After 120 days, Group II goats showed no regression of the changes in their reproductive behavior or improvement of their seminal parameters. The main defects observed in the sperm of goats that consumed I. brasiliana were cytoplasmatic droplets, bent tails and detached tails. The main histopathological findings were reported in tests, with cytoplasmic vacuolization of germline and Sertoli cells, generalized impairment of spermatogonia maturation with exfoliation of degenerative cells, cell fragments, rare abnormal spermatocytes in the seminiferous lumen and disappearance of Leydig cells. Results of this study confirmed the hypothesis that I. brasiliana causes testicular degeneration in male goats.(AU)

Este trabalho teve como objetivo caracterizar as mudanças comportamentais relacionadas a reprodução e lesões no sistema reprodutor de caprinos intoxicados por Ipomoea brasiliana. A planta foi coletada e administrada na dose de 4g/kg (800g swainsonina/kg) para dois grupos de caprinos durante 45 dias. Os caprinos do Grupo I foram eutanasiados no 46º dia do experimento e os caprinos do Grupo II no 120º dia. O Grupo III foi constituído por caprinos que não receberam I. brasiliana e foram eutanasiados no 120º dia de experimento. Alterações comportamentais reprodutivas foram observadas a partir de 20 dias de experimento e consistiram em ausência do comportamento de corte, ausência de reflexo de Flehmen, diminuição ou perda de libido e incapacidade de realizar a monta natural. Após 120 dias, os caprinos do Grupo II não apresentaram regressão de alterações reprodutivas. Os principais defeitos observados no sêmen dos caprinos que consumiam I. brasiliana foram gotas citoplasmáticas, caudas dobradas e caudas destacadas. Os principais achados histopatológicos consistiram em vacuolização citoplasmática das células da linhagem germinativa e células de Sertoli; comprometimento generalizado da maturação das espermatogônias com esfoliação de células degeneradas; presença de fragmentos celulares e raros espermatócitos anormais no lúmen dos túbulos seminíferos e ausência de células de Leydig. Os resultados confirmam a hipótese de que o consumo de I. brasiliana causa degeneração testicular em caprinos.(AU)

Numeric and volumetric changes in Leydig cells during aging of rats

Purpose: To analyze the effects of aging in rats on the nuclear volume, cytoplasmic volume, and total volume of Leydig cells, as well as their number. Methods: Seventy-two Wistar rats were divided into six subgroups of 12 rats, which underwent right orchiectomy at 3, 6, 9, 12, 18, and 24 months of age. The weight and volume of the resected testicles were assessed. A stereological study of Leydig cells was conducted, which included measurements of cell number and nuclear, cytoplasmic, and total cell volumes. Results: The weight and volume of the resected testicles showed reductions with age. Only the subgroup composed of 24-month old rats showed a decrease in the nuclear volume of Leydig cells. Significant reductions in the cytoplasmic volume and total volume of Leydig cells were observed in 18- and 24-month old rats. The number of Leydig cells did not vary significantly with age. Conclusions: Aging in rats resulted in reduction of the nuclear, cytoplasmic, and total cell volumes of Leydig cells. There was no change in the total number of these cells during aging.(AU)

Bovine fetal testicular development in the Nellore breed / Desenvolvimento testicular fetal bovino na raça Nelore

The study of gonadal development improves the understanding of factors that can influence the reproductive development process. This study aims to characterize bovine fetal testicular development and the testosterone level in the Nellore breed. For the study, 162 bovine fetuses aged between 3 and 8 months were collected from Nellore cows at a local abattoir. The fetal age was estimated by DP=8.4+0.087L+5.46?L, where DP is the estimated pregnancy day and L represents fetal length. The fetal gonadal weight (g), width (cm), and thickness (cm) were measured. Thereafter, the gonads were submitted to classic histology processes in 3-µm-thick slices cut at 210 µm intervals. The Sertoli cells, Leydig cells, and germ cells were counted. Blood samples were collected from umbilical cords for testosterone levels. The data were analyzed using the Spearman correlation test followed by Principal Component Analysis and one-way ANOVA to compare the averages between months. The testicular weight and volume were found to have a positive correlation with the numbers of Sertoli cells (r = 0.84; p 0.0001 and r = 0.92; p 0.0001, respectively), Leydig cells (r = 0.80; p 0.0001 and r = 0.90; p 0.0001, respectively), and germ cells (r = 0.84; p 0.0001 and r = 0.93; p 0.0001, respectively) and to be negatively correlated with testosterone plasmatic concentration (r = -0.31; p = 0.0001 andr = -0.22; p = 0.006, respectively) during pregnancy. After the fifth month, the numbers of Sertolicells, Leydig cells and germ cells differed (p < 0.0001) from the following gestational months. Thehighest testosterone concentration (p = 0.007) was observed in the fifth month of gestation and wasfollowed by a concentration decrease in the seventh and eighth months. The increase in cell quantitywas responsible for the increase in testicular weight and volume during fetal development. On the otherhand...

O estudo do desenvolvimento gonadal melhora o entendimento dos fatores que podem influenciar o processo de desenvolvimento reprodutivo. Esse estudo objetivou caracterizar o desenvolvimento testicular bovino e os níveis de testosterona na raça Nelore. Para o estudo, 162 fetos bovinos com idade entre 3 e 8 meses foram coletados de vacas Nelore em frigorífico local. A idade fetal foi estimada pela fórmula DP=8.4+0.087L+5.46?L, onde DP corresponde ao dia estimado de gestação e L representa o comprimento fetal. Foram medidos o peso gonadal fetal (g), a largura (cm) e a espessura (cm). Após as mensurações, as gônadas foram submetidas ao processamento histológico clássico, sendo utilizadas para elaboração das lâminas cortes com 3 µm de espessura com intervalos entre cortes de 210 µm. As células de Sertoli, Leydig e germinativas foram contadas. Amostras de sangue foram coletadas do cordão umbilical para quantificação dos níveis de testosterona. Os dados foram analisados utilizando a correlação de Spearman seguida pela análise de componentes principais, sendo o comparativo de médias entre os meses realizados utilizando o one-way ANOVA. O peso e o volume testicular apresentaram correlação positiva com o número de células de Sertoli (r = 0,84; p 0,0001 e r = 0,92; p 0,0001, respectivamente), células de Leydig (r = 0,80; p 0,0001 e r = 0,90; p 0,0001, respectivamente), porém foramnegativamente correlacionados com a concentração plasmática de testosterona (r = -0,31; p = 0,0001e r = -0,22; p = 0,006, respectivamente) durante a gestação. Após o quinto mês, o número de célulasde Sertoli, de Leydig e germinativas diferiram (p < 0,0001) dos meses seguintes de gestação. A maiorconcentração de testosterona encontrada (p < 0,007) foi observada no quinto mês de gestação, sendoseguida por uma diminuição entre o sétimo e oitavo mês. O aumento na quantidade de células duranteo desenvolvimento fetal foi o responsável pelo aumento do peso e volume testicular...

Bovine fetal testicular development in the Nellore breed / Desenvolvimento testicular fetal bovino na raça Nelore

The study of gonadal development improves the understanding of factors that can influence the reproductive development process. This study aims to characterize bovine fetal testicular development and the testosterone level in the Nellore breed. For the study, 162 bovine fetuses aged between 3 and 8 months were collected from Nellore cows at a local abattoir. The fetal age was estimated by DP=8.4+0.087L+5.46?L, where DP is the estimated pregnancy day and L represents fetal length. The fetal gonadal weight (g), width (cm), and thickness (cm) were measured. Thereafter, the gonads were submitted to classic histology processes in 3-µm-thick slices cut at 210 µm intervals. The Sertoli cells, Leydig cells, and germ cells were counted. Blood samples were collected from umbilical cords for testosterone levels. The data were analyzed using the Spearman correlation test followed by Principal Component Analysis and one-way ANOVA to compare the averages between months. The testicular weight and volume were found to have a positive correlation with the numbers of Sertoli cells (r = 0.84; p 0.0001 and r = 0.92; p 0.0001, respectively), Leydig cells (r = 0.80; p 0.0001 and r = 0.90; p 0.0001, respectively), and germ cells (r = 0.84; p 0.0001 and r = 0.93; p 0.0001, respectively) and to be negatively correlated with testosterone plasmatic concentration (r = -0.31; p = 0.0001 andr = -0.22; p = 0.006, respectively) during pregnancy. After the fifth month, the numbers of Sertolicells, Leydig cells and germ cells differed (p < 0.0001) from the following gestational months. Thehighest testosterone concentration (p = 0.007) was observed in the fifth month of gestation and wasfollowed by a concentration decrease in the seventh and eighth months. The increase in cell quantitywas responsible for the increase in testicular weight and volume during fetal development. On the otherhand...(AU)

O estudo do desenvolvimento gonadal melhora o entendimento dos fatores que podem influenciar o processo de desenvolvimento reprodutivo. Esse estudo objetivou caracterizar o desenvolvimento testicular bovino e os níveis de testosterona na raça Nelore. Para o estudo, 162 fetos bovinos com idade entre 3 e 8 meses foram coletados de vacas Nelore em frigorífico local. A idade fetal foi estimada pela fórmula DP=8.4+0.087L+5.46?L, onde DP corresponde ao dia estimado de gestação e L representa o comprimento fetal. Foram medidos o peso gonadal fetal (g), a largura (cm) e a espessura (cm). Após as mensurações, as gônadas foram submetidas ao processamento histológico clássico, sendo utilizadas para elaboração das lâminas cortes com 3 µm de espessura com intervalos entre cortes de 210 µm. As células de Sertoli, Leydig e germinativas foram contadas. Amostras de sangue foram coletadas do cordão umbilical para quantificação dos níveis de testosterona. Os dados foram analisados utilizando a correlação de Spearman seguida pela análise de componentes principais, sendo o comparativo de médias entre os meses realizados utilizando o one-way ANOVA. O peso e o volume testicular apresentaram correlação positiva com o número de células de Sertoli (r = 0,84; p 0,0001 e r = 0,92; p 0,0001, respectivamente), células de Leydig (r = 0,80; p 0,0001 e r = 0,90; p 0,0001, respectivamente), porém foramnegativamente correlacionados com a concentração plasmática de testosterona (r = -0,31; p = 0,0001e r = -0,22; p = 0,006, respectivamente) durante a gestação. Após o quinto mês, o número de célulasde Sertoli, de Leydig e germinativas diferiram (p < 0,0001) dos meses seguintes de gestação. A maiorconcentração de testosterona encontrada (p < 0,007) foi observada no quinto mês de gestação, sendoseguida por uma diminuição entre o sétimo e oitavo mês. O aumento na quantidade de células duranteo desenvolvimento fetal foi o responsável pelo aumento do peso e volume testicular...(AU)