The evaluation of clinicopathological and immunohistochemical findings of cervical clear cell carcinoma in a bitch

Background: Clear cell carcinoma (CCC) of the cervix is a rarely seen malignant tumor which is classified in adenocarcinomas of the endometrium. It is responsible for 2.15-3.0% of endometrial tumors. The diagnosis is difficult and is still controversial in uterine carcinomas. Macroscopically, masses were protruding to endometrial surface. However, the definitive diagnosis was carried by microscopical evaluation. The cells contain clear cytoplasm. The cells are seen in this pattern because there is either glycogenation or secretoric activity in the cells.Case: In the present case, clinicopathologic and immunohistochemical findings of clear cell carcinoma of the cervix were defined in a 3 year-old spayed Golden retriever bitch. Previously spayed bitch, which had vaginal discharge for the last month-long, was submitted to the Department of Obstetrics and Gynecology, Ankara University, Faculty of Veterinary Medicine. In the general clinical examination, vaginoscopy was performed and vaginal cytology was obtained from smear. The source of discharge was detected as cervix uteri. In vaginal cytologic examination, erythrocyte, neutrophile and superficial cells were observed. In abdominal ultrasonography, a hypoechoic mass was detected at cranial part of the urinary bladder. To evaluate the general health condition of the dog, total blood counting and serum biochemistry were analyzed in addition to assessing its hormone prophlye. Estrogen and progesterone levels were evaluated. Estradiol (E2) level was measured as 23 pg/mL and progesterone level was measured as 1.96 ng/mL from collected serum. The cervical mass in diameters of 3x4x2.5 cm was removed in operation. In macroscopical examination, it had spherical and regularly shape. After the macroscopical examination, tissue samples were fixed in 10% buffered formalin. Then, the samples stained with Haematoxylin-Eosin (H&E), Masson’s trichrome and PAS stainings, respectively.[...]

The evaluation of clinicopathological and immunohistochemical findings of cervical clear cell carcinoma in a bitch

Background: Clear cell carcinoma (CCC) of the cervix is a rarely seen malignant tumor which is classified in adenocarcinomas of the endometrium. It is responsible for 2.15-3.0% of endometrial tumors. The diagnosis is difficult and is still controversial in uterine carcinomas. Macroscopically, masses were protruding to endometrial surface. However, the definitive diagnosis was carried by microscopical evaluation. The cells contain clear cytoplasm. The cells are seen in this pattern because there is either glycogenation or secretoric activity in the cells.Case: In the present case, clinicopathologic and immunohistochemical findings of clear cell carcinoma of the cervix were defined in a 3 year-old spayed Golden retriever bitch. Previously spayed bitch, which had vaginal discharge for the last month-long, was submitted to the Department of Obstetrics and Gynecology, Ankara University, Faculty of Veterinary Medicine. In the general clinical examination, vaginoscopy was performed and vaginal cytology was obtained from smear. The source of discharge was detected as cervix uteri. In vaginal cytologic examination, erythrocyte, neutrophile and superficial cells were observed. In abdominal ultrasonography, a hypoechoic mass was detected at cranial part of the urinary bladder. To evaluate the general health condition of the dog, total blood counting and serum biochemistry were analyzed in addition to assessing its hormone prophlye. Estrogen and progesterone levels were evaluated. Estradiol (E2) level was measured as 23 pg/mL and progesterone level was measured as 1.96 ng/mL from collected serum. The cervical mass in diameters of 3x4x2.5 cm was removed in operation. In macroscopical examination, it had spherical and regularly shape. After the macroscopical examination, tissue samples were fixed in 10% buffered formalin. Then, the samples stained with Haematoxylin-Eosin (H&E), Massons trichrome and PAS stainings, respectively.[...](AU)

Avaliação dos indicadores de qualidade de laboratórios de citopatologia cervical

Neste trabalho foi avaliado o desempenho dos 13 laboratórios que realizaram o exame citopatológico para o SUS no Paraná, por meio de seis indicadores do monitoramento interno da qualidade (MIQ). Foi realizado estudo retrospectivo, baseado nos dados obtidos no programa do Ministério da Saúde, SISCOLO/CNES, dos laboratórios que efetuaram acima de 1.500 exames de Papanicolaou/ano, de janeiro de 2008 a dezembro de 2012. A avaliação da qualidade dos laboratórios foi feita pela análise do índice de positividade (IP), percentuais de exames compatíveis com ASC entre os exames satisfatórios, ASC entre os exames alterados, exames compatíveis com HSIL, exames insatisfatórios e razão ASC/SIL. Dos laboratórios avaliados, apenas um apresentou produtividade maior que 15 mil exames/ano conforme recomendação da QualiCito, e correspondeu a 82,9 % dos exames realizados no estado. O IP mostrou que 46,1 % dos laboratórios apresentaram resultado muito baixo, 38,5 % baixo, apenas 18,1 % dentro da faixa esperada. Com relação ao percentual de HSIL, 23,1 % dos laboratórios apresentaram percentual igual ou superior a 0,4 %. Portanto, os resultados deste estudo mostraram que os indicadores de qualidade do MIQ, dos laboratórios que realizaram exame citopatológico para o SUS, estão abaixo dos parâmetros recomendado pelo Ministério da Saúde.(AU)

This study aimed at evaluating the profile of 13 laboratories that perform the Pap smear for the Public Healthcare System in Paraná State, Brazil, through six internal quality monitoring indicators (MIQ). This retrospective study was based on the data obtained from the Ministry of Health Program, SISCOLO/ CNES, including laboratories performing over 1500 Pap tests/year, from January 2008 to April 2013. The quality assessment of laboratories was performed by analyzing the positivity rate (PR), the percentage of tests compatible with ASC among satisfactory examinations, the ASC among abnormal tests, the tests compatible with HSIL, the unsatisfactory rate and the ASC/SIL ratio. Of analyzed laboratories, only one demonstrated productivity higher than 15.000 tests/year as recommended by QualiCito, and these corresponded to 82.9 % of tests performed in the State. The PR was very low in 46.1 % of laboratories and low in 38.5 %; 18.1 % of laboratories only were within the expected range. The percentage of HSIL was equal to or greater than 0.4 % in 23.1 % of laboratories. Therefore, this study showed that the quality indicators of MIQ of the laboratories performing the cervical cancer screening are below the parameters recommended by the Ministry of Health.(AU)

High incidence of oncogenic HPV genotypes found in women from Southern Brazil

Oncogenic HPV genotypes are strongly associated with premalignant and malignant cervical lesion. The purpose was to determine human papillomavirus (HPV) prevalence and genotypes, and to estimate cervical cancer risk factor associations. Cervical samples were obtained from 251 women seeking gynecological care at the Pelotas School of Medicine Clinic. This is a cross-sectional study. HPV-DNA was amplified by nested-PCR using MY09/11 and GP5/6 primers, and the sequencing was used for genotyping. Sociodemographic and behavioral risk factors were obtained by closed questionnaire, and its relationship to HPV infection prevalence were analyzed. Statistical analyses were performed using SPSS 16.0 software, and differences were considered significant at p < 0.05. As results, the prevalence of HPV infection was 29.9%. The most frequent genotype was HPV-16 (41.3%), followed by HPV-18 (17.3%), and HPV-33 (9.3%). Others nine HPV genotypes were also found. On this population, prevalence of oncogenic HPV genotypes was high, but does not seem to confer relationship with the risk factors investigated. Future investigations in larger populations are necessary, for the proposition of more appropriated monitoring strategies and treatment according to the Brazilian health service reality, as well as patients.

Immunohistochemistry of the uterine cervix of rats bearing the Walker 256 tumor treated with copaiba balsam

PURPOSE: To investigate the immunohistochemistry of the uterine cervix of 20 Wistar rats (Rattus norvegicus) bearing the Walker 256 tumor, treated with copaiba oil (Copaifera officinalis). METHODS: The animals were grouped into four subgroups, with five rats each: the GCT and GCopT received distilled water and topically copaiba, respectively, while the GCG and GCopG received distilled water and copaiba by gavage, respectively. The substances were administered for nine days. On the 12th day, after euthanasia, the tumor pieces were sent to the identification of T CD4+, T CD8+ and Natural Killer cells. RESULTS: It was found that the pattern of expression for specific markers of phenotypes of cells involved in tumor immune response was similar in all groups, regardless the administration way of copaiba oil (topical or gavage). CONCLUSION: Copaiba balsam, administered either topically or by gavage, did not alter the pattern of tumor immune response in rats bearing Walker 256 Tumor.(AU)

Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus

Background : The venom of Centruroides limpidus limpidus (Cll) is a mixture of pharmacologically active principles. The most important of these are toxic proteins that interact both selectively and specifically with different cellular targets such as ion channels. Recently, anticancer properties of the venom from other scorpion species have been described. Studies in vitro have shown that scorpion venom induces cell death, inhibits proliferation and triggers the apoptotic pathway in different cancer cell lines. Herein, after treating human cervical adenocarcinoma (HeLa) cells with Cll crude venom, their cytotoxic activity and apoptosis induction were assessed. Results : Cll crude venom induced cell death in normal macrophages in a dose-dependent manner. However, through viability assays, HeLa cells showed high survival rates after exposure to Cll venom. Also, Cll venom did not induce apoptosis after performing ethidium bromide/acridine orange assays, nor was there any evidence of chromatin condensation or DNA fragmentation. Conclusions : Crude Cll venom exposure was not detrimental to HeLa cell cultures. This may be partially attributable to the absence of specific HeLa cell membrane targets for molecules present in the venom of Centruroides limpidus limpidus. Although these results might discourage additional studies exploring the potential of Cll venom to treat human papilloma cervical cancer, further research is required to explore positive effects of crude Cll venom on other cancer cell lines.(AU)

The effect of copaiba balsam on Walker 256 carcinoma inoculated into the vagina and uterine cervix of female rats / Efeito do óleo de copaíba no tumor de Walker 256 inoculado em vagina e colo de útero de ratas fêmeas

Purpose: To verify the copaiba balsam (Copaifera officinalis) effect on Walker 256 carcinoma inoculated into vagina and uterine cervix of rats. Methods: Eighteen female Wistar rats weighing between 180-250g were used, distributed into 2 groups (GCop, GC). On the 1st day of the experiment, 0.3 ml of Walker 256 carcinoma (2x10(6) concentration) was inoculated in both groups; on the 3rd day of the experiment, it was given 4.8 ml/kg of distilled water to the GC group, and 4.8 ml/kg of copaiba balsam to the GCop group. On the 12th day, euthanasia was performed and the tumor was grafted, being weighted and verified its volume. The data were submitted to statistical analysis with ANOVA test. Results: It was observed that copaiba balsam presented a negative inhibitory potential of 70 percent. Conclusion: The copaiba balsam stimulated the tumor growth.(AU)

Objetivo: Verificar o efeito do óleo de copaíba da espécie Copaifera officinalis no carcinoma de Walker 256 inoculado em vagina e colo de útero de ratas. Métodos: Foram utilizadas 18 ratas da linhagem Wistar, pesando entre 180-250g, distribuídas em dois grupos (CCop, GC). No 1º dia de experimento, em ambos os grupos foi inoculado 0,3ml de tumor de Walker 256 na concentração de 2x10(6); no 3º dia após essa inoculação, foi iniciada a administração de água destilada na dose de 4,8 ml/kg ao GC, e copaíba na dose de 4,8 ml/kg ao GCop. No 12º dia foi realizada a eutanásia das ratas e ressecado o tumor, sendo este pesado e averiguado seu volume. Os dados obtidos foram submetidos à análise estatística pelo método ANOVA. Resultados: Observou-se que o óleo de copaíba apresentou um potencial inibitório negativo de 70 por cento. Conclusão: O óleo de copaíba estimulou o crescimento tumoral.(AU)

Perfil humoral da toxoplasmose em pacientes oncológicos

Toxoplasma gondii é um protozoário com uma ampla distribuição em todo o mundo, agente causador da toxoplasmose, uma infecção oportunista, que vem acometendo de forma considerável imunocomprometidos. Pacientes com câncer estão predispostos a adquirir a infecção, seja ela recente ou devido a uma reativação. No entanto, poucos dados existem sobre este protozoário nesta população. O objetivo do estudo foi verificar o perfil humoral de T.gondii em pacientes oncológicos do Hospital Escola da Universidade Federal de Pelotas e do Centro de Radioterapia e Oncologia do Hospital Santa Casa de Misericórdia de Pelotas, Rio Grande do Sul, durante o tratamento quimioterápico, além de correlacionar com aspectos clínicos, epidemiológicos, laboratoriais e de verificar a possível associação do parasito com alguns tipos de câncer. Foi realizado um estudo prospectivo, e um total de 77 pacientes oncológicos foram acompanhados durante três ciclos de tratamento. Para traçar o perfil humoral de T.gondii, três amostras foram estabelecidas, de acordo com o surgimento de anticorpos IgG e IgM, e a pesquisa sorológica foi realizada através da técnica de Eletroquimioluminescência. Os dados clínicos foram obtidos através da análise do prontuário dos pacientes, enquanto que os epidemiológicos, pela aplicação de um questionário. Foram usados também os resultados dos exames fornecidos pelos laboratórios de Análises Clínicas das respectivas instituições. Constatou-se que a soropositividade para IgG foi de 90,9% e para IgM, 7,8%. Ao avaliar o perfil humoral, 64,9% dos pacientes apresentaram um aumento de anticorpos IgG, sendo que, em 50% dos casos, esse aumento foi de duas ou mais vezes. Através da análise dos fatores de risco (p<0,05), a variável idade foi estatisticamente significativa. Pacientes com câncer de pulmão, esôfago e colo do útero e os que possuíam metástase apresentaram, respectivamente, um Risco Relativo (RR) 2,54 (1,38 < RR < 4,70) e 4,17 (2,17 < RR < 8,03) mais chance de apresentarem este aumento de anticorpos. Este estudo revela a importância do monitoramento para toxoplasmose em pacientes oncológicos, já que, pelo quadro de imunossupressão, eles estão suscetíveis a desenvolverem a parasitose por uma infeção recente ou reativação, o que poderia evitar, assim, consequências graves ou até mesmo fatais.

Toxoplasma gondii is a protozoan that is broadly distributed all over the world, an agent that causes toxoplasmosis, an opportunist infection that has been considerably affecting immunocompromised patients. Cancer patients are predisposed to acquire the infection, being it recent or caused by a reactivation. However, there is little data about this protozoan in that population. The objective of this study was to verify the humoral profile of T.gondii on oncological patients from the School Hospital of Federal University of Pelotas and from the Oncology and Radiotherapy Centre of Santa Casa de Misericórdia Hospital of Pelotas, in the state of Rio Grande do Sul, during chemotherapy treatment. Besides, it was done a correlation between that profile and clinical and epidemiologic aspects as well as laboratory tests results, and it was verified a possible association of the parasite to some types of cancer. A prospective study was conducted, and 77 oncological patients were followed during three cycles of treatment. In order to draw the humoral profile of T.gondii, three samples have been established, according to the appearance of antibodies IgG and IgM, and the serologic research was done through Electrochemiluminescence technique. Clinical data has been gathered by analyzing patients records, while epidemiological data has been gathered by a questionnaire and the results of exams that were provided by the laboratories of clinical analysis of each institution. Seropositive results for IgG was 90%, and for IgM, 7,8%. Through the evaluation of humoral profile, 64,9% of patients presented an increase of IgG antibodies, and 50% of those patients doubled or even presented a bigger increase of IgG antibodies than that.. Through the analysis of risk factors (p<0,05), the variable age was statistically significant. Patients with lung, esophagus, and cervical cancer, and the ones that had metastasis presented, respectively, a Relative Risk (RR) 2,54 (1,38 < RR < 4,70) and 4,17 (2,17 < RR < 8,03) more chance of presenting that increase of antibodies. This study shows the importance of toxoplasmosis monitoring in oncological patients, since because of their immunosuppression state, hey are susceptible to developing the parasitosis by a recent infection or a reactivation. The monitoring could avoid, this way, serious, or even fatal consequences.

Human papillomavirus infection in women attended at a cervical cancer screening service in Natal, Brazil

We analyzed cervical specimens of 202 women, aged 15 to 64 years, attended at Luis Antonio Hospital, Natal, Brazil, to determine the prevalence of HPV and identify the more frequent genotypes and risk factors for HPV infection in women attended at a cervical cancer screening service. Two specimens were collected from each patient: one for cytological examination and the other to detect HPV DNA by PCR, and typing by dot blot hybridization. A total of 54.5% of the sample had normal cytology and 45.5% had cytological alterations. HPV was detected in 24.5% of the cytologically normal women and in 59.8% of those with altered cytology. Both single and double HPV infection increased the likelihood of cytological alterations. Thirteen types of HPV were identified, most of which were high risk. HPV 16 was the most prevalent single-type infection, followed by HPV 58. The most frequent double infection was the association between HPV 56 and 57. The prevalence of HPV in cytologically normal women was greater than that reported for countries on all the continents except Africa. The inverse was observed in women with cytological alterations. The distribution of HPV types was similar to that described for the Americas, with some differences. Multiple sexual partners was the only risk factor showing an association with the presence of HPV infection.

Foram analisados espécimes cervicais de 202 mulheres, com idade variando de 15 e 64 anos, atendidas no Hospital Luis Antonio, Natal-RN, objetivando determinar a prevalência do HPV, identificar genotipos do vírus e possíveis fatores de risco para a infecção por esse patógeno, em mulheres atendidas em um serviço de rastreamento do câncer do colo do útero. De cada paciente foram coletados dois espécimes: um destinado ao exame citológico e outro para detecção do HPV por PCR, com tipagem por hibridização "dot blot". Das pacientes incluídas no estudo, 54,5% apresentaram citologia normal e 45,5% tinham alterações citológicas. HPV foi detectado em 24,5% das mulheres com citologia normal, e em 59,8% das que apresentaram citologia alterada. Tanto a infecção por um único tipo de HPV, quanto a infecção simultânea por dois tipos diferentes do vírus aumentaram a chance de ocorrência de alterações citológicas. Foram identificados treze tipos de HPV, a maioria de alto risco. Nas infecções por um único tipo, o HPV 16 foi o mais prevalente, seguido do HPV 58. Na infecção dupla a associação mais freqüente foi entre HPV 56 e 57. A prevalência do HPV nas mulheres com citologia normal foi superior a relatada para países de todos os continentes, exceto África. Nas mulheres com alterações citológicas observou-se resultado inverso. A distribuição dos tipos de HPV foi semelhante à descrita para Américas, com algumas diferenças. Dos fatores de risco analisados, o relacionamento sexual com múltiplos parceiros foi o único que apresentou associação com a presença de infecção por HPV.

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats / Modelo experimental de Tumor de Walker 256 em vagina e colo de útero de ratas

PURPOSE: To establish an inoculation model of Walker 256 carcinoma on cervix uteri and vagina of rats. METHODS: Fifteen female rats were used, and assigned to three groups each one with five rats: group A - rats with 4x10(6) cells of Walker 256 carcinoma without acid acetic inoculation; group B - rats with 2x10(6) cells of Walker 256 carcinoma with acid acetic inoculation and group C: rats with 4x10(6) cells of Walker 256 carcinoma with acid acetic inoculation. The day before tumor cells inoculation the rats from groups B and C were anaesthetized with diethylether and 0,3 ml of acetic acid was inoculated into their vaginas. Tumor cell inoculation into the vagina and cervix was done under general anesthesia with diethylether. Then a endocervical brush was used to scrape the vaginal wall and after that 0,3 ml of the liquid containing tumor cells was inoculated on the vagina and cervix. For the tumor analysis, animals were euthanized at day 12 following tumor cell implantation by an excessive inhalation of diethylether. Tumor was resected entirely and weighed and the tumors were then sectioned and counter stained with hematoxylin and eosin for histopathologic evaluation. It was also calculated the percentage of tumor equivalent to the body weight by the formula: P= tumor weight / body weight x 100. Data were analyzed by one-way analysis of variance - ANOVA. P values < 0.05 were taken to indicate statistical significance. RESULTS: Implantation and growth on GB and GC was 100 percent and on GA 20 percent. There was no statistical difference between GB and GC averages. CONCLUSION: According to the methods used, the Walker 256 carcinoma inoculation model into vagina and cervix have an implantation and growth rate of 100 percent when associated with previous acid acetic inoculation and there is no behavioral difference between using 2x10(6) or 4x10(6) cells on its inoculation(AU)

OBJETIVO: Estabelecer um modelo de inoculação de Tumor de Walker 256 em vagina e colo de útero de ratas. MÉTODOS: Foram utilizadas 15 ratas fêmeas, virgens, adultas, pesando entre 200-250g, distribuídas em três grupos de estudo com cinco animais cada: grupo A (GA): ratas com tumor de Walker 256 em concentração de 4x10(6) sem ácido acético; grupo B (GB): ratas com tumor de Walker 256 em concentração de 2x10(6) células com ácido acético; grupo C (GC): ratas com tumor de Walker 256 em concentração de 4x10(6) células com ácido acético. No dia anterior à inoculação do tumor, foi realizada a inoculação de 0,3 ml de ácido acético a 10 por cento na vagina das ratas de GB e GC; no dia seguinte, tanto estas como as ratas do grupo GA foram anestesiadas, feita a escarificação da parede vaginal com uma escova de endocérvice e inoculado 0,3ml de tumor na concentração de 4x10(6) células nos grupos GA e GC e 2x10(6) células no grupo GB. Após 12 dias, foi realizada a eutanásia e removido o tumor em bloco com vagina e cornos uterinos para análise, sendo pesado e averiguado seu volume e calculado as relações entre o seu peso e o peso final da rata e o seu volume e o peso final da rata. Os dados foram colhidos e submetidos à análise estatística pelo método ANOVA (um critério). RESULTADOS: A pega em GB e GC foi 100 por cento e em GA 20 por cento. Não houve diferença estatística entre as médias obtidas entre GB e GC. CONCLUSÃO: De acordo com a metodologia utilizada, o modelo de tumor de Walker 256 na vagina apresenta pega de 100 por cento quando associado a ácido acético e não há diferença de comportamento com a inoculação de 4x10(6)ou 2x10(6) células(AU)

Comparative study of the results found in preventive examinations and in uterine cervix cancer screening procedures performed with Brazilian women / Comparação dos resultados de exames preventivos e de rastreamento de câncer de colo do útero em mulheres brasileiras

This study assesses the results from preventives examinations and cervical cancer screenings carried out, between January 2005 and July 2008, at a private gynecological clinic and at an agreed health service located in Florianópolis-SC, whose data were recorded in a reference file. These data were analyzed in parallel with those results from preventive exams performed by the Public Health System of Florianópolis in the same period, available at the DATASUS site. The majority of diagnosis results considered normal were found in patients examined at the private and at the agreed service clinic. Nonetheless, the samples analyzed by SUS (Brazilian public health system) showed a lower frequency of atypical squamous cells of unknown significance, such as cervical intraepithelial neoplasia 1, 2 and 3, Human papilomavirus (HPV) and Candida spp. than those reported by the private clinic. It was evidenced that the examinations carried out at the private and agreed gynecological clinic showed less severe cytopathogenic changes as compared to the results recorded by SUS examinations. This variation on cytopathogenicity findings might occurr due to socio-economic differences and periodicity of examinations.

Foram avaliados os resultados dos exames preventivos e realizado o rastreamento de câncer de colo de útero, registrados em uma clínica ginecológica de atendimento particular e conveniado, comparando-os com os dados do Sistema Único de Saúde (SUS). Os exames preventivos de colo de útero realizados no período de janeiro de 2005 a julho de 2008, em clínica ginecológica de atendimento particular e conveniado de Florianópolis SC, foram analisados, por meio de preenchimento de cadastro. Esses dados foram comparados com os resultados dos exames preventivos realizados pelo SUS na cidade de Florianópolis no mesmo período, disponíveis no site do DATASUS. A maioria dos exames realizados na clínica particular e conveniado ao SUS de Florianópolis apresentou resultados dentro da normalidade. Contudo, as amostras registradas no SUS demonstraram menor frequência de alterações escamosas atípicas de significado indeterminado, possivelmente não neoplásico, neoplasia intraepitelial cervical grau 1, 2 e 3, Papilomavírus humano (HPV) e Candida spp., quando comparados com os dados obtidos em clínica de atendimento particular. Os exames citopatológicos realizados pela clínica particular registraram resultados de menor gravidade do que as análises realizadas pelo SUS. A variação nas características observadas pode ter ocorrido em função das diferenças socioeconômicas e da periodicidade do exame.

Phytochemical profile and biological activities of Bromelia antiacantha extracts / Perfil fitoquímico e atividades biológicas de extratos de Bromelia antiacantha

Reports from popular medicine usually act as a basis for the development of new drugs from natural compounds with therapeutic actions for serious diseases and prevalence such as cancer. Bromelia antiacantha Bertol. is a species of the Bromeliaceae family, considered an unconventional food plant, found in the south and midwest regions of Brazil. Despite the high nutritional content and pharmacological potential of its fruits, few scientific studies report its biological actions. Thus, this study evaluates the phytochemical profile of aqueous and ethanol extracts obtained from B. antiacantha fruits, as well as their possible antioxidant, antitumor, and cytotoxic activities. The aqueous extract exhibited phenolic compounds and flavonoids, while ethanol extracts indicated the presence of flavonoids and coumarin in their composition, regardless of the region of collection. The ethanolic extract demonstrated a more promising antioxidant effect than the aqueous extract and also induced a significant inhibition in the viability of human cervical cancer cells of the SiHa strain. In addition, treatment with both extracts did not alter the viability of non-tumor cells of the immortalized human keratinocyte lineage (HaCaT). These results bring new data about extracts obtained from a native plant, edible and traditionally used in popular medicine, opening new perspectives for its possible therapeutic application.

Relatos da medicina popular costumam atuar como referencial para o desenvolvimento de novos fármacos a partir de moléculas naturais com ações terapêuticas para doenças de alta gravidade e prevalência como o câncer. Bromelia antiacantha Bertol. é uma espécie da família Bromeliaceae, considerada uma planta alimentícia não convencional (PANC), encontrada nas regiões sul e centro-oeste do Brasil. Apesar do alto teor nutritivo e potencial farmacológico de seus frutos, poucos estudos científicos relatam suas ações biológicas. Desta forma, este estudo avalia o perfil fitoquímico de extratos aquoso e etanólico obtidos de frutos de B. antiacantha, bem como a sua possível ação antioxidante, antitumoral e citotóxica. O extrato aquoso apresentou compostos fenólicos e flavonoides, enquanto os extratos etanólicos apontam a presença de flavonóides e cumarina em sua composição, independente da região de coleta. O extrato etanólico demonstrou efeito antioxidante mais promissor do que o extrato aquoso e também induziu uma inibição significativa na viabilidade de células humanas de câncer cervical da linhagem SiHa. Além disso, o tratamento com ambos extratos não alterou a viabilidade de células não tumorais da linhagem de queratinócitos humanos imortalizados (HaCaT). Estes dados trazem novas informações sobre extratos obtidos de uma espécie vegetal nativa, comestível e já utilizada tradicionalmente, mas abrindo novas perspectivas quanto a possíveis aplicações terapêuticas.