Tecnologia para produção de extrato aquoso de folhas da planta Solanum lycocarpum para avaliação da eficiencia anti-helmíntica: validação da técnica de inibição da eclodibilidade larvar de helmintos de ovinos / Technology for production of aqueous extract of plant leaves Solanum lycocarpum for anthelmintic efficacy evaluation: validation of the larval hatchability inhibition technique of sheep helminths / Tecnología para la producción de extracto acuoso de hojas de la planta de Solanum lycocarpum para la evaluación de la eficiencia anti helmíntica: validación de la técnica de inhibición de larvar eclodibilidad de helmintos de oveja

A criação de ovinos possui grande importância sócio-econômica e cultural para o estado de Mato Grosso do Sul e vem sendo amplamente explorada, por meio da criação de raças adaptadas ao clima da região e ao mercado consumidor, sendo a produção impulsionada principalmente por meio da necessidade de diversificação das atividades produtivas no meio rural. Apesar do incremento produtivo para o setor, ainda hoje existem entraves à otimização do desempenho global dos rebanhos ovinos, dentre eles está a helmintose e resistência anti-parasitária à alopáticos. Paralelamente a estas, estudos relacionados a utilização de fitoterápicos para o tratamento parasitário estão sendo desenvolvidos e uma planta em especial, Solanum Lycocarpum, desponta para o tratamento anti-helmíntico sendo sua eficiência contra parasitas em animais silvestres e disponibilidade fatores favoráveis. Objetivou-se com a execução deste trabalho realizar a fabricação de extrato aquoso das folhas da planta Solanum Lycocarpum obtido por infusão e testar este frente a eficiência anti-helmíntica por meio da utilização da técnica de inibição da eclodibilidade larvar adaptada às condições de execução. Os resultados obtidos demonstram a viabilidade produtiva do extrato aquoso de folhas da planta Solanum Lycocarpum e a eficiência da utilização deste por meio da técnica de eclodibilidade larvar modificada, possibilitando redução de 83,33% da eclosão dos ovos de parasitas gastrointestinais de um ovino naturalmente infestado de propriedade de Dourados ­ MS.(AU)

Sheep breeding corresponds to a factor of great socio-economic and cultural importance for the state of Mato Grosso do Sul and has been widely exploited, increasing its production chain of sheep breeding breeds adapted to the climate of the region and the consumer market; being the production driven mainly by the need to diversify productive activities in rural areas. Despite the productive increase for the sector, there are still barriers to optimize the overall performance of sheep flocks, among them is helminthosis and antiparasitic resistance to allopaths. Parallel to these, studies related to the use of phytotherapics for parasite treatment are being developed and one plant in particular, Solanum Lycocarpum, stands out for anthelmintic treatment being its efficiency in wild animals and availability favorable factors. The purpose of this work was to manufacture aqueous extracts from the leaves of the Solanum Lycocarpum plant obtained by pressure and to test its anthelmintic efficiency by using the validation of the larval hatchability inhibition technique. The results obtained demonstrate the productive viability of Solanum Lycocarpum aqueous leaf extract and its efficient use by means of the modified larval hatchability technique, enabling an 83.33% reduction in the hatching of eggs from gastrointestinal parasites in a naturally infested sheep owned by Dourados ­ MS.(AU)

La cría de ovinos tiene una gran importancia socio-económica y cultural para el estado de Mato Grosso do Sul y ha sido ampliamente explorada a través de la creación de razas adaptadas al clima de la región y al mercado consumidor, siendo la producción impulsada principalmente por la necesidad de diversificación productiva. actividades en las zonas rurales. A pesar del aumento de la producción para el sector, aún existen obstáculos para optimizar el rendimiento general de los rebaños de ovinos, incluidos los helmintos y la resistencia antiparasitaria a los alopáticos. Paralelamente, se están desarrollando estudios relacionados con el uso de fitoterápicos para el tratamiento parasitario y está emergiendo una planta en particular, Solanum Lycocarpum, para el tratamiento antihelmíntico, su eficacia frente a parásitos en animales silvestres y factores favorables a la disponibilidad. El objetivo de este trabajo fue realizar la fabricación de un extracto acuoso de las hojas de la planta de Solanum Lycocarpum obtenido por infusión y contrastarlo frente a la eficacia antihelmíntica mediante el uso de la técnica de inhibición de la incubabilidad larvaria adaptada a las condiciones de ejecución. Los resultados obtenidos demuestran la viabilidad productiva del extracto acuoso de las hojas de la planta de Solanum Lycocarpum y la eficiencia de su uso mediante la técnica de incubabilidad larvaria modificada, permitiendo una reducción del 83,33% de la eclosión de huevos de parásitos gastrointestinales de una oveja infestada naturalmente. propiedad de Dourados - MS.(AU)

Assessment of weight gain and control of parasitism by Rhipicephalus microplus in dairy cattle in the field using organosynthetic parasiticide, phytotherapics and homeopathy / Avaliação do ganho de peso e controle do parasitismo por Rhipicephalus microplus em bovinos leiteiros a campo usando parasiticida organosintético, fitoterápicos e homeopatia

The goal of this research was to compare the effect of the following products on dairy cattle, parasitized by Rhipicephalus microplus: organosynthetics - Clorfenvinfós and Ivermectina (OG); phytotherapics - Eucalyptus oil (EG) and Neem cake (NG); Homeopathy (HG); The control group (CG) received no treatment. Infestation was by R. microplus (MIC) and weight gain (GP) were evaluated. The study included 60 animals (5 groups) from ­ Dutch / Zebu, aged between 25-44 months and initial weight between 211-477kg. EG and NG showed MIC 84.9% and 14.0% greater than CG, respectively (P <0.05; P = 0). HG and OG had MIC less than CG 24.4% and 16.9%, respectively (P<0.05; P <0.05). EG, NG and OG obtained GP lower than CG in 7.9%, 8.9% and 8.06% (P <0.05) respectively. The HG GP was 4.9% higher (P <0.05) than CG. This was the first research to prove parasitism control by R. microplus in a field test, using homeopathy. The methodology for choosing Homeopathy for the control of enzooties, developed and tested for the first time in this study, proved to be adequate and efficient, opening the possibility for establishing a new methodology for strategic control of parasitism by R. microplus.(AU)

O objetivo desta pesquisa foi comparar o efeito dos seguintes produtos sobre bovinos leiteiros, parasitados por Rhipicephalus microplus: organossintéticos - clorfenvinfós e ivermectina (OG); fitoterápicos - óleo de eucalipto (EG) e torta de Neem (NG) e homeopatia (HG). O grupo controle (CG) não recebeu tratamento. Foram avaliados infestação por R. microplus (MIC) e ganho de peso/grupo (GP) em 60 animais (5 grupos), a partir de ­ holandês/zebu, com idade entre 25 e 44 meses e peso inicial entre 211 e 477kg. EG e NG apresentaram MIC 84,9% e 14,0% maior que CG, respectivamente (P<0,05; P=0). HG e OG apresentaram MIC menor que CG 24,4% e 16,9%, respectivamente (P<0,05; P<0,05). EG, NG e OG obtiveram GP menor que CG em 7,9%, 8,9% e 8,06% (P<0,05), respectivamente. O GP do HG foi 4,9% maior (P<0,05) que o CG. Esta foi a primeira pesquisa a comprovar controle do parasitismo por R. microplus em teste a campo, usando homeopatia. A metodologia para escolha de medicamentos homeopáticos para controle de enzootias, desenvolvida e testada pela primeira vez neste estudo, mostrou-se adequada e eficiente, abrindo a possibilidade para estabelecimento de nova metodologia para controle estratégico do parasitismo por R. microplus.(AU)

Situational diagnosis of professionals of family health units on phytotherapy / Diagnóstico situacional dos profissionais de unidades de saúde da família sobre a fitoterapia

The incorporation of the use of phytotherapy in the primary health care is an important gain for the Unified Health System, besides reducing the cost in the treatment of the patient, rescues the popular knowledge and promotes the rational use of the medicinal plants. To determine the situational diagnostic profile of professionals of family health units on phytotherapy. A quantitative observational study was carried out, in which forms were applied to the team of the family health units of the municipality of Marechal Deodoro da Fonseca/Alagoas. Only 23% of the interviewed professionals reported having had contact with scientific information on phytotherapy during professional training. All of them approve the implantation of the official practice of phytotherapy and routinely prescribe phytotherapics and medicinal plants, but they do not know the possible risks of drug interaction with the use of medicinal plants. Health professionals Health professionals support the official practice of phytotherapy in public health and show interest in participating in courses / training on the subject to better serve the population.(AU)

A incorporação do uso da fitoterapia na atenção primária à saúde é um ganho importante para o Sistema Único de Saúde, além da redução do custo no tratamento do paciente, resgata o conhecimento popular e promove o uso racional das plantas medicinas. Determinar o perfil diagnóstico situacional dos profissionais de unidades de saúde da família sobre fitoterapia. Foi realizado um estudo observacional quantitativo, no qual foram aplicados formulários para a equipe das unidades de saúde da família do município de Marechal Deodoro da Fonseca/Alagoas. Apenas 23% dos profissionais entrevistados afirmaram ter tido contato com informações científicas sobre fitoterapia durante a formação profissional. Todos aprovam a implantação da prática oficial da fitoterapia e prescrevem rotineiramente fitoterápicos e plantas medicinais, entretanto desconhecem os possíveis riscos de interação medicamentosa com o uso das plantas medicinas. Os profissionais de saúde apoiam a prática oficial da fitoterapia na saúde pública e demonstram interesse em participar de cursos/treinamentos sobre a temática para melhor atender a população.(AU)

Situational diagnosis of professionals of family health units on phytotherapy

Abstract The incorporation of the use of phytotherapy in the primary health care is an important gain for the Unified Health System, besides reducing the cost in the treatment of the patient, rescues the popular knowledge and promotes the rational use of the medicinal plants. To determine the situational diagnostic profile of professionals of family health units on phytotherapy. A quantitative observational study was carried out, in which forms were applied to the team of the family health units of the municipality of Marechal Deodoro da Fonseca/Alagoas. Only 23% of the interviewed professionals reported having had contact with scientific information on phytotherapy during professional training. All of them approve the implantation of the official practice of phytotherapy and routinely prescribe phytotherapics and medicinal plants, but they do not know the possible risks of drug interaction with the use of medicinal plants. Health professionals Health professionals support the official practice of phytotherapy in public health and show interest in participating in courses / training on the subject to better serve the population.

Resumo A incorporação do uso da fitoterapia na atenção primária à saúde é um ganho importante para o Sistema Único de Saúde, além da redução do custo no tratamento do paciente, resgata o conhecimento popular e promove o uso racional das plantas medicinas. Determinar o perfil diagnóstico situacional dos profissionais de unidades de saúde da família sobre fitoterapia. Foi realizado um estudo observacional quantitativo, no qual foram aplicados formulários para a equipe das unidades de saúde da família do município de Marechal Deodoro da Fonseca/Alagoas. Apenas 23% dos profissionais entrevistados afirmaram ter tido contato com informações científicas sobre fitoterapia durante a formação profissional. Todos aprovam a implantação da prática oficial da fitoterapia e prescrevem rotineiramente fitoterápicos e plantas medicinais, entretanto desconhecem os possíveis riscos de interação medicamentosa com o uso das plantas medicinas. Os profissionais de saúde apoiam a prática oficial da fitoterapia na saúde pública e demonstram interesse em participar de cursos/treinamentos sobre a temática para melhor atender a população.

Utilização de creme de extrato de calêndula em ferida lacerante em equino / Use of calender extract cream in equine lacerate wound

Background: Wounds and lacerations are constant in the clinical routine of horses. The treatment of wounds by the secondintention is the most common in the equine clinic; it consists in addition to surgical debridement, the use of allopathic orphytotherapeutic healing. In this context, several phytotherapics used in folk medicine have been used with promisingresults in the equine species, especially marigold (Calendula officinalis L.), recognized by the German scientific committee as antiseptic and healing. The objective of this study was to evaluate the efficacy of the nonionic cream of Calendulaofficinalis at 2% in an extensive lacerating wound in an equine.Case: A 6-month-old female Quarter Horse, weighing 160 kg, a lacerating wound in the pectoral region of unknowntraumatic origin, was treated in the large animal sector of the Veterinary Hospital of Universidade Paranaense (UNIPAR).Clinical examination revealed an exudative wound in the pectoral region, complicated by the presence of environmentalorganic matter and myiasis, involving transverse pectoral and descending pectoral muscles, with a large tissue separationand exposure of part of the first sternum. After an initial evaluation, a trichotomy was performed around the wound, removalof myiasis and sanitization of the wound with water and PVP-I, which was filled with sterile dressings and covered with adressing using a tubular mesh. Antibiotic therapy was instituted, along with anti-inflammatory medication and antitetanicserum (5,000 IU). Due to the need for surgical debridement, after previous fasting and general anesthesia followed bysite antisepsis, surgical debridement was performed, followed by reduction of dead space with application of standard“Wolf” sutures, for re-approximation of the wound edges was applied suture in Wolf pattern captonated with segmentsof equipment. In the post-surgical period, topical treatment with nitrofurazone ointment and crystal...

Utilização de creme de extrato de calêndula em ferida lacerante em equino / Use of calender extract cream in equine lacerate wound

Background: Wounds and lacerations are constant in the clinical routine of horses. The treatment of wounds by the secondintention is the most common in the equine clinic; it consists in addition to surgical debridement, the use of allopathic orphytotherapeutic healing. In this context, several phytotherapics used in folk medicine have been used with promisingresults in the equine species, especially marigold (Calendula officinalis L.), recognized by the German scientific committee as antiseptic and healing. The objective of this study was to evaluate the efficacy of the nonionic cream of Calendulaofficinalis at 2% in an extensive lacerating wound in an equine.Case: A 6-month-old female Quarter Horse, weighing 160 kg, a lacerating wound in the pectoral region of unknowntraumatic origin, was treated in the large animal sector of the Veterinary Hospital of Universidade Paranaense (UNIPAR).Clinical examination revealed an exudative wound in the pectoral region, complicated by the presence of environmentalorganic matter and myiasis, involving transverse pectoral and descending pectoral muscles, with a large tissue separationand exposure of part of the first sternum. After an initial evaluation, a trichotomy was performed around the wound, removalof myiasis and sanitization of the wound with water and PVP-I, which was filled with sterile dressings and covered with adressing using a tubular mesh. Antibiotic therapy was instituted, along with anti-inflammatory medication and antitetanicserum (5,000 IU). Due to the need for surgical debridement, after previous fasting and general anesthesia followed bysite antisepsis, surgical debridement was performed, followed by reduction of dead space with application of standard“Wolf” sutures, for re-approximation of the wound edges was applied suture in Wolf pattern captonated with segmentsof equipment. In the post-surgical period, topical treatment with nitrofurazone ointment and crystal...(AU)

Use of phytotherapics, low power laser and ozone for biting wound in dog

Background: Skin wounds are among the most common cases in small animals, highlighting the importance of alternativetherapies as an aid to the healing process. These include ozone, which has a bactericidal and immunomodulatory action; thelow power laser that determines photodynamic changes triggering metabolism elevation; and the use of medicinal plants,in which several herbal medicines has been mentioned in research relating their benefits to wound healing in humans andanimals. Thus, it aims to report the case of a dog submitted to these treatments due to chronic wound due to bite.Case: A 6-year-old canine, female, mixed-breed, 8 kg, with a perforating wound in the left anterior limb in the scapularand humeral region, caused by trauma from the bite of another dog, previously treated, without clinical improvement,has been attended presenting apathy, increased body temperature (40.1°C), difficulty in walking and inability to supportthe injured limb, which was swollen, hyperemic, with elevated temperature, absence of hair, elevated pain sensitivity andpresence of 2 circular lesions, approximately 2 cm in diameter, caused by trauma, and others of size between 0.5 and 1 cmin diameter, which appeared during the course of the disease, characterized as fistulas, through which there was drainageof characteristic fluid exudative and purulent discharge. It was collected blood sample for blood count and purulent material through sterile swab for culture and antibiogram. Systemic treatment with ceftiofur, dipyrone, meloxican and topicalcleaning of the wounds with hydrogen peroxide and physiological solution; dimethyl sulfoxide ointment, and hot compresses was chosen. After seven days, the animal showed significant general clinical...

Use of phytotherapics, low power laser and ozone for biting wound in dog

Background: Skin wounds are among the most common cases in small animals, highlighting the importance of alternativetherapies as an aid to the healing process. These include ozone, which has a bactericidal and immunomodulatory action; thelow power laser that determines photodynamic changes triggering metabolism elevation; and the use of medicinal plants,in which several herbal medicines has been mentioned in research relating their benefits to wound healing in humans andanimals. Thus, it aims to report the case of a dog submitted to these treatments due to chronic wound due to bite.Case: A 6-year-old canine, female, mixed-breed, 8 kg, with a perforating wound in the left anterior limb in the scapularand humeral region, caused by trauma from the bite of another dog, previously treated, without clinical improvement,has been attended presenting apathy, increased body temperature (40.1°C), difficulty in walking and inability to supportthe injured limb, which was swollen, hyperemic, with elevated temperature, absence of hair, elevated pain sensitivity andpresence of 2 circular lesions, approximately 2 cm in diameter, caused by trauma, and others of size between 0.5 and 1 cmin diameter, which appeared during the course of the disease, characterized as fistulas, through which there was drainageof characteristic fluid exudative and purulent discharge. It was collected blood sample for blood count and purulent material through sterile swab for culture and antibiogram. Systemic treatment with ceftiofur, dipyrone, meloxican and topicalcleaning of the wounds with hydrogen peroxide and physiological solution; dimethyl sulfoxide ointment, and hot compresses was chosen. After seven days, the animal showed significant general clinical...(AU)

In vitro and in vivo antiparasitic action of essential oils of Lippia spp. in Koi Carp (Cyprinus carpio) fed supplemented diets

This study evaluated the in vitro antiparasitic activity of the essential oils of Lippia alba, L. origanoides and L. sidoides against monogenean parasites of koi carp Cyprinus carpio and its zootechnical performance in net cages. The oils were obtained from the leaves by hydro distillation, and the chemical composition was evaluated via gas chromatography. In vitro assays were performed with each essential oil separately and combined in binary (1:1) and tertiary (1:1:1) mixtures with the Lippia species at 10, 20, 40, 60, 80 and 100 mg L-1 and two control groups (grain alcohol and tank water). To determine zootechnical performance, L. sidoides oil was added to the feed at 0.00 (control), 0.25, 0.50, 0.75 and 1%, in triplicate and with 20 fish per net cage fed for 60 days. The best results in vitro against monogeneans were observed for L. sidoides (40 mg L-1 in 8 min), followed by L. origanoides (40 mg L-1 in 25 min) and L. alba (40 mg L-1 in 4 h). Reductions in weight gain, protein efficiency rate and specific growth rate were observed in diets containing 0.75% of L. sidoides oil in comparison to the control and the 0.25% diet. There were no significant differences in growth, individual mean feed intake, apparent feed conversion and parasitological indices. Based on our results, 0.25% L. sidoides oil showed the best zootechnical performance, but was not effective against koi carp parasites in vivo.

Cicatrização por segunda intenção de feridas cutâneas em ratos Wistar com uso de Stryphnodendron adstringens / Screening for second intention of cutaneous wounds in rats Wistar with use of Stryphnodendron adstringens

Pesquisas recentes vêm descrevendo que o fitoterápico composto pelo extrato da casca do barbatimão contém compostos fenólicos, que conferem ação antimicrobiana, antioxidante e adstringente por se ligarem a proteínas e polissacarídeos, sugerindo propriedades cicatrizantes. O presente estudo objetivou avaliar a cicatrização por segunda intenção de feridas cutâneas tratadas combarbatimão. Foram utilizados 15 ratos machos e hígidos, submetidos a duas incisões: Ferida Controle tratada com solução fisiológica a 0,9% e a Ferida Teste, tratada com pomada de barbatimão. Os animais foram subdivididos em três subgrupos com cinco ratos cada: G1 biopsados ao 3º dia pós-cirúrgico, G2 ao 7º dia e G3 ao 14º dia. A avaliação das feridas foi feita do ponto de vista macro e microscópico nos períodos pré-determinados. Após a biópsia, foram realizados cortes histológicos corados pela Hematoxilina Eosina e Tricômico de Masson. Referente aos achados macroscópicos notou-se que no 3° dia não houve alterações significativas; no 7° dia observou-se epitelização parcial e ausência de crosta nas Feridas Teste; ao 14° dia, as Feridas Controle apresentaram prurido e epitelização completa e a Ferida Teste apresentou epitelização parcial sem prurido. Quanto às alterações histopatológicas, observou-se que as células inflamatórias das Feridas Controle com 3°, 7° e 14° dias, mostraram-se aumentadas em relação à Ferida Teste. Quanto aos fibroblastos e a colagenização, não apresentaram alteração ao 3º, 7º e 14º dia pós-operatório. A reorganização das fibras houve distinção biológica entre os 3°, 7° e 14° dias. A epitelização no 3° dia não apresentou alterações significativas, no 7° dia, a Ferida Teste mostrou epitelização parcial e no 14° dia, ambas a feridas mostraram epitelização completa. Conclui-se que o tratamento se mostrou mais eficiente devido à supressão da inflamação...

Recent research has described that the herbal extract of barbatimão contains phenoliccompounds, that confer antimicrobial, antioxidant and astringent action by binding toproteins and polysaccharides, suggesting healing properties. In this sense, the present studyaimed to evaluate the second intention healing of skin wounds treated with barbatimão. Fifteen healthy male rats were used and submitted to two incisions: the Control Wound treated with 0.9% physiological solution and the Test Wound treated with barbatimão ointment. The animals were subdivided into three subgroups with five rats each: G1 biopsied on the 3rd postoperative day, G2 on day 7 and G3 on day 14. The evaluation of the wounds was done from the macro and microscopic point of view in the pre-determined periods. Histological sections stained by Hematoxylin Eosin and Masson's Tricommium were performed after the biopsy. Regarding the macroscopic findings, it was noted that onthe 3rd day there were no significant changes; on the 7th day, partial epithelialization and absence of crust on the Test Wounds were observed; at the 14th day the Control Wounds presented pruritus and complete epithelization and the Test Wound presented partial epithelialization without pruritus. As for the histopathological changes, it was observed that the inflammatory cells of Control Wounds with 3rd, 7th and 14th days were increased in relation to the Test Wound. As for fibroblasts and collagenization, they did not present alterations at the 3rd, 7th and 14th postoperative days. The reorganization of the fibers had a biological distinction between the 3rd, 7th and 14th days. Epithelialization on the 3rd day did not show any significant changes, on the 7th day the test wound showed partial epithelialization, on the 14th day both wounds showed complete epithelization...

Cicatrização por segunda intenção de feridas cutâneas em ratos Wistar com uso de Stryphnodendron adstringens / Screening for second intention of cutaneous wounds in rats Wistar with use of Stryphnodendron adstringens

Pesquisas recentes vêm descrevendo que o fitoterápico composto pelo extrato da casca do barbatimão contém compostos fenólicos, que conferem ação antimicrobiana, antioxidante e adstringente por se ligarem a proteínas e polissacarídeos, sugerindo propriedades cicatrizantes. O presente estudo objetivou avaliar a cicatrização por segunda intenção de feridas cutâneas tratadas combarbatimão. Foram utilizados 15 ratos machos e hígidos, submetidos a duas incisões: Ferida Controle tratada com solução fisiológica a 0,9% e a Ferida Teste, tratada com pomada de barbatimão. Os animais foram subdivididos em três subgrupos com cinco ratos cada: G1 biopsados ao 3º dia pós-cirúrgico, G2 ao 7º dia e G3 ao 14º dia. A avaliação das feridas foi feita do ponto de vista macro e microscópico nos períodos pré-determinados. Após a biópsia, foram realizados cortes histológicos corados pela Hematoxilina Eosina e Tricômico de Masson. Referente aos achados macroscópicos notou-se que no 3° dia não houve alterações significativas; no 7° dia observou-se epitelização parcial e ausência de crosta nas Feridas Teste; ao 14° dia, as Feridas Controle apresentaram prurido e epitelização completa e a Ferida Teste apresentou epitelização parcial sem prurido. Quanto às alterações histopatológicas, observou-se que as células inflamatórias das Feridas Controle com 3°, 7° e 14° dias, mostraram-se aumentadas em relação à Ferida Teste. Quanto aos fibroblastos e a colagenização, não apresentaram alteração ao 3º, 7º e 14º dia pós-operatório. A reorganização das fibras houve distinção biológica entre os 3°, 7° e 14° dias. A epitelização no 3° dia não apresentou alterações significativas, no 7° dia, a Ferida Teste mostrou epitelização parcial e no 14° dia, ambas a feridas mostraram epitelização completa. Conclui-se que o tratamento se mostrou mais eficiente devido à supressão da inflamação...(AU)

Recent research has described that the herbal extract of barbatimão contains phenoliccompounds, that confer antimicrobial, antioxidant and astringent action by binding toproteins and polysaccharides, suggesting healing properties. In this sense, the present studyaimed to evaluate the second intention healing of skin wounds treated with barbatimão. Fifteen healthy male rats were used and submitted to two incisions: the Control Wound treated with 0.9% physiological solution and the Test Wound treated with barbatimão ointment. The animals were subdivided into three subgroups with five rats each: G1 biopsied on the 3rd postoperative day, G2 on day 7 and G3 on day 14. The evaluation of the wounds was done from the macro and microscopic point of view in the pre-determined periods. Histological sections stained by Hematoxylin Eosin and Masson's Tricommium were performed after the biopsy. Regarding the macroscopic findings, it was noted that onthe 3rd day there were no significant changes; on the 7th day, partial epithelialization and absence of crust on the Test Wounds were observed; at the 14th day the Control Wounds presented pruritus and complete epithelization and the Test Wound presented partial epithelialization without pruritus. As for the histopathological changes, it was observed that the inflammatory cells of Control Wounds with 3rd, 7th and 14th days were increased in relation to the Test Wound. As for fibroblasts and collagenization, they did not present alterations at the 3rd, 7th and 14th postoperative days. The reorganization of the fibers had a biological distinction between the 3rd, 7th and 14th days. Epithelialization on the 3rd day did not show any significant changes, on the 7th day the test wound showed partial epithelialization, on the 14th day both wounds showed complete epithelization...(AU)

In vitro and in vivo antiparasitic action of essential oils of Lippia spp. in Koi Carp (Cyprinus carpio) fed supplemented diets

This study evaluated the in vitro antiparasitic activity of the essential oils of Lippia alba, L. origanoides and L. sidoides against monogenean parasites of koi carp Cyprinus carpio and its zootechnical performance in net cages. The oils were obtained from the leaves by hydro distillation, and the chemical composition was evaluated via gas chromatography. In vitro assays were performed with each essential oil separately and combined in binary (1:1) and tertiary (1:1:1) mixtures with the Lippia species at 10, 20, 40, 60, 80 and 100 mg L-1 and two control groups (grain alcohol and tank water). To determine zootechnical performance, L. sidoides oil was added to the feed at 0.00 (control), 0.25, 0.50, 0.75 and 1%, in triplicate and with 20 fish per net cage fed for 60 days. The best results in vitro against monogeneans were observed for L. sidoides (40 mg L-1 in 8 min), followed by L. origanoides (40 mg L-1 in 25 min) and L. alba (40 mg L-1 in 4 h). Reductions in weight gain, protein efficiency rate and specific growth rate were observed in diets containing 0.75% of L. sidoides oil in comparison to the control and the 0.25% diet. There were no significant differences in growth, individual mean feed intake, apparent feed conversion and parasitological indices. Based on our results, 0.25% L. sidoides oil showed the best zootechnical performance, but was not effective against koi carp parasites in vivo.(AU)

Atividade acaricida de diferentes solventes sobre fêmeas ingurgitadas e larvas de Rhipicephalus microplus

ABSTRACT: Cattle ticks cause economic losses to the cattle industry in Brazil. The use of phytotherapics as acaricides has shown efficacy of the control of these parasites. In the present study, the in vitro efficacy of the following drugs was evaluated: dimethyl sulfoxide (DMSO), ethanol, methanol, propylene glycol and polyethylene glycol sorbitan monolaurate at dilutions 100%, 20%, 10%, 5%, 1% e 0.5%. The toxic effect of these solvents on engorged female and larvae of Rhipicephalus microplus was analyzed. The immersion test was used to define the reproductive efficiency of engorged R. microplus females and efficiency of the drugs. The immersion test larvae modified syringe was used to analyze of the susceptibility of tick larvae. Propylene glycol and DMSO was used on engorged females and DMSO, ethanol, methanol and propylene glycol, all at concentration of 0.5%, were use on larvae the, showed the lowest mortality rate; this indicate that its use at low concentrations as solvent acaricide in the control of R. microplus could be recommended.

RESUMO: Os carrapatos causam perdas econômicas à indústria pecuária no Brasil. Dessa forma, o uso de fitoterápicos como acaricidas mostrou eficácia no controle desses parasitas. Neste estudo, foi avaliada a eficácia in vitro dos seguintes fármacos: dimetil sulfóxido (DMSO), etanol, metanol, propilenoglicol e monolaurato de sorbitan polietilenoglicol em diluições de 100%, 20%, 10%, 5%, 1% e 0,5%. O efeito tóxico destes solventes em fêmeas e larvas ingurgitadas de Rhipicephalus microplus foi analisado. O teste de biocarrapaticidograma, foi utilizado para definir a eficiência reprodutiva das teleóginas de R. microplus e a eficiência dos produtos testados. Para análise da susceptibilidade das larvas foi utilizado o teste por imersão de larvas em seringa modificado. O uso de propilenoglicol e DMSO em fêmeas ingurgitadas e o uso de DMSO, etanol, metanol e propilenoglicol em larvas, todos à concentração de 0,5%, apresentaram uma menor taxa de mortalidade, podendo ter seu uso indicado em baixas concentrações como solventes de acaricidas utilizados no controle do R. microplus.

Reduction of doxorubicin-induced genotoxicity by Handroanthus impetiginosus in mouse bone marrow revealed by micronucleus assay

Abstract Handroanthus impetiginosus has long been used in traditional medicine and various studies have determined the presence of bioactive chemical compounds and potential phytotherapeutics. In this study, the genotoxicity of the lyophilized tincture of H. impetiginosus bark (THI) was evaluated in mouse bone marrow using micronucleus assays. The interaction between THI and genotoxic effects induced by the chemotherapeutic agent, doxorubicin (DXR), was also analyzed. Experimental groups were evaluated 24 to 48 h after treatment with N-nitroso-N-ethylurea (NEU; 50 mg/kg), DXR (5 mg/kg), sodium chloride (NaCl; 150 mM), and THI (0.5-2 g/kg). Antigenotoxic assays were carried out using THI (0.5 g/kg) in combination with NEU or DXR. Analysis of the micronucleated polychromatic erythrocytes (MNPCEs) indicated no significant differences between treatment doses of THI (0.5-2 g/kg) and NaCl. Polychromatic erythrocyte (PCE) to normochromatic erythrocyte (NCE) ratios did not indicate any statistical differences between DXR and THI or NaCl, but there were differences between THI and NaCl. A significant reduction in MNPCEs and PCE/NCE ratios was observed when THI was administered in combination with DXR. This study suggested the absence of THI genotoxicity that was dose-, time-, and gender-independent and the presence of moderate systemic toxicity that was dose-independent, but time- and gender-dependent. The combination of THI and DXR also suggested antigenotoxic effects, indicating that THI reduced genotoxic effects induced by chemotherapeutic agents.

Resumo Handroanthus impetiginosus tem sido usada durante um longo período pela medicina tradicional e vários estudos têm demonstrados a presença de compostos químicos e potencial fitoterapêutico. Esta pesquisa avaliou a genotoxicidade da tintura da casca liofilizada de H. impetiginosus (THI) usando o ensaio do micronúcleo em medula óssea de camundongos. A interação entre THI e os efeitos genotóxicos induzidos pelo quimioterápico doxorrubicina (DXR) também foram analisados. Grupos experimentais foram analisados a 24-48 h após o tratamento com N-Nitroso-N-etiluréia (NEU; 50 mg/kg), DXR (5 mg/kg), NaCl (150 mM) e THI (0,5-2 g/kg). O ensaio antigenotóxico foi conduzido utilizando THI (0,5 g/kg) em combinação com NEU ou DXR. A análise de eritrócitos policromáticos micronucleados (EPCMNs) não mostrou diferenças significativas entre as doses de tratamento (0,5-2 g/kg) e NaCl. As proporções de eritrócitos policromáticos (EPC)/eritrócitos normocromáticos (ENC) não revelaram diferenças estatísticas entre DXR e THI ou NaCl, porém houve diferenças entre THI e NaCl. Uma redução significativa em EPCMNs e na razão EPC/ENC foi observada quando THI foi administrado em combinação com DXR. Essa pesquisa sugere ausência de genotoxicidade de THI, dose-, tempo- e sexo-independente, e moderada toxicidade sistêmica dose-independente, mas tempo- e sexo-dependente. A associação do THI e DXR também sugere efeitos antigenotóxicos. Por conseguinte, THI pode reduzir os efeitos genotóxicos induzidos pelo quimioterapêutico.

Reduction of doxorubicin-induced genotoxicity by Handroanthus impetiginosus in mouse bone marrow revealed by micronucleus assay

Abstract Handroanthus impetiginosus has long been used in traditional medicine and various studies have determined the presence of bioactive chemical compounds and potential phytotherapeutics. In this study, the genotoxicity of the lyophilized tincture of H. impetiginosus bark (THI) was evaluated in mouse bone marrow using micronucleus assays. The interaction between THI and genotoxic effects induced by the chemotherapeutic agent, doxorubicin (DXR), was also analyzed. Experimental groups were evaluated 24 to 48 h after treatment with N-nitroso-N-ethylurea (NEU; 50 mg/kg), DXR (5 mg/kg), sodium chloride (NaCl; 150 mM), and THI (0.5-2 g/kg). Antigenotoxic assays were carried out using THI (0.5 g/kg) in combination with NEU or DXR. Analysis of the micronucleated polychromatic erythrocytes (MNPCEs) indicated no significant differences between treatment doses of THI (0.5-2 g/kg) and NaCl. Polychromatic erythrocyte (PCE) to normochromatic erythrocyte (NCE) ratios did not indicate any statistical differences between DXR and THI or NaCl, but there were differences between THI and NaCl. A significant reduction in MNPCEs and PCE/NCE ratios was observed when THI was administered in combination with DXR. This study suggested the absence of THI genotoxicity that was dose-, time-, and gender-independent and the presence of moderate systemic toxicity that was dose-independent, but time- and gender-dependent. The combination of THI and DXR also suggested antigenotoxic effects, indicating that THI reduced genotoxic effects induced by chemotherapeutic agents.

Resumo Handroanthus impetiginosus tem sido usada durante um longo período pela medicina tradicional e vários estudos têm demonstrados a presença de compostos químicos e potencial fitoterapêutico. Esta pesquisa avaliou a genotoxicidade da tintura da casca liofilizada de H. impetiginosus (THI) usando o ensaio do micronúcleo em medula óssea de camundongos. A interação entre THI e os efeitos genotóxicos induzidos pelo quimioterápico doxorrubicina (DXR) também foram analisados. Grupos experimentais foram analisados a 24-48 h após o tratamento com N-Nitroso-N-etiluréia (NEU; 50 mg/kg), DXR (5 mg/kg), NaCl (150 mM) e THI (0,5-2 g/kg). O ensaio antigenotóxico foi conduzido utilizando THI (0,5 g/kg) em combinação com NEU ou DXR. A análise de eritrócitos policromáticos micronucleados (EPCMNs) não mostrou diferenças significativas entre as doses de tratamento (0,5-2 g/kg) e NaCl. As proporções de eritrócitos policromáticos (EPC)/eritrócitos normocromáticos (ENC) não revelaram diferenças estatísticas entre DXR e THI ou NaCl, porém houve diferenças entre THI e NaCl. Uma redução significativa em EPCMNs e na razão EPC/ENC foi observada quando THI foi administrado em combinação com DXR. Essa pesquisa sugere ausência de genotoxicidade de THI, dose-, tempo- e sexo-independente, e moderada toxicidade sistêmica dose-independente, mas tempo- e sexo-dependente. A associação do THI e DXR também sugere efeitos antigenotóxicos. Por conseguinte, THI pode reduzir os efeitos genotóxicos induzidos pelo quimioterapêutico.

Atividade acaricida de diferentes solventes sobre fêmeas ingurgitadas e larvas de Rhipicephalus microplus / Acaricidal activity of different solvents on engorged females and larvae of Rhipicephalus microplus

Os carrapatos causam perdas econômicas à indústria pecuária no Brasil. Dessa forma, o uso de fitoterápicos como acaricidas mostrou eficácia no controle desses parasitas. Neste estudo, foi avaliada a eficácia in vitro dos seguintes fármacos: dimetil sulfóxido (DMSO), etanol, metanol, propilenoglicol e monolaurato de sorbitan polietilenoglicol em diluições de 100%, 20%, 10%, 5%, 1% e 0,5%. O efeito tóxico destes solventes em fêmeas e larvas ingurgitadas de Rhipicephalus microplus foi analisado. O teste de biocarrapaticidograma, foi utilizado para definir a eficiência reprodutiva das teleóginas de R. microplus e a eficiência dos produtos testados. Para análise da susceptibilidade das larvas foi utilizado o teste por imersão de larvas em seringa modificado. O uso de propilenoglicol e DMSO em fêmeas ingurgitadas e o uso de DMSO, etanol, metanol e propilenoglicol em larvas, todos à concentração de 0,5%, apresentaram uma menor taxa de mortalidade, podendo ter seu uso indicado em baixas concentrações como solventes de acaricidas utilizados no controle do R. microplus.(AU)

Cattle ticks cause economic losses to the cattle industry in Brazil. The use of phytotherapics as acaricides has shown efficacy of the control of these parasites. In the present study, the in vitro efficacy of the following drugs was evaluated: dimethyl sulfoxide (DMSO), ethanol, methanol, propylene glycol and polyethylene glycol sorbitan monolaurate at dilutions 100%, 20%, 10%, 5%, 1% e 0.5%. The toxic effect of these solvents on engorged female and larvae of Rhipicephalus microplus was analyzed. The immersion test was used to define the reproductive efficiency of engorged R. microplus females and efficiency of the drugs. The immersion test larvae modified syringe was used to analyze of the susceptibility of tick larvae. Propylene glycol and DMSO was used on engorged females and DMSO, ethanol, methanol and propylene glycol, all at concentration of 0.5%, were use on larvae the, showed the lowest mortality rate; this indicate that its use at low concentrations as solvent acaricide in the control of R. microplus could be recommended.(AU)

Atividade acaricida de diferentes solventes sobre fêmeas ingurgitadas e larvas de Rhipicephalus microplus / Acaricidal activity of different solvents on engorged females and larvae of Rhipicephalus microplus

Os carrapatos causam perdas econômicas à indústria pecuária no Brasil. Dessa forma, o uso de fitoterápicos como acaricidas mostrou eficácia no controle desses parasitas. Neste estudo, foi avaliada a eficácia in vitro dos seguintes fármacos: dimetil sulfóxido (DMSO), etanol, metanol, propilenoglicol e monolaurato de sorbitan polietilenoglicol em diluições de 100%, 20%, 10%, 5%, 1% e 0,5%. O efeito tóxico destes solventes em fêmeas e larvas ingurgitadas de Rhipicephalus microplus foi analisado. O teste de biocarrapaticidograma, foi utilizado para definir a eficiência reprodutiva das teleóginas de R. microplus e a eficiência dos produtos testados. Para análise da susceptibilidade das larvas foi utilizado o teste por imersão de larvas em seringa modificado. O uso de propilenoglicol e DMSO em fêmeas ingurgitadas e o uso de DMSO, etanol, metanol e propilenoglicol em larvas, todos à concentração de 0,5%, apresentaram uma menor taxa de mortalidade, podendo ter seu uso indicado em baixas concentrações como solventes de acaricidas utilizados no controle do R. microplus.(AU)

Cattle ticks cause economic losses to the cattle industry in Brazil. The use of phytotherapics as acaricides has shown efficacy of the control of these parasites. In the present study, the in vitro efficacy of the following drugs was evaluated: dimethyl sulfoxide (DMSO), ethanol, methanol, propylene glycol and polyethylene glycol sorbitan monolaurate at dilutions 100%, 20%, 10%, 5%, 1% e 0.5%. The toxic effect of these solvents on engorged female and larvae of Rhipicephalus microplus was analyzed. The immersion test was used to define the reproductive efficiency of engorged R. microplus females and efficiency of the drugs. The immersion test larvae modified syringe was used to analyze of the susceptibility of tick larvae. Propylene glycol and DMSO was used on engorged females and DMSO, ethanol, methanol and propylene glycol, all at concentration of 0.5%, were use on larvae the, showed the lowest mortality rate; this indicate that its use at low concentrations as solvent acaricide in the control of R. microplus could be recommended.(AU)

Reduction of doxorubicin-induced genotoxicity by Handroanthus impetiginosus in mouse bone marrow revealed by micronucleus assay / Redução da genotoxicidade induzida pela doxorrubicina por Handroanthus impetiginosus na medula óssea de camundongos revelada pelo ensaio do micronúcleo

Handroanthus impetiginosus has long been used in traditional medicine and various studies have determined the presence of bioactive chemical compounds and potential phytotherapeutics. In this study, the genotoxicity of the lyophilized tincture of H. impetiginosus bark (THI) was evaluated in mouse bone marrow using micronucleus assays. The interaction between THI and genotoxic effects induced by the chemotherapeutic agent, doxorubicin (DXR), was also analyzed. Experimental groups were evaluated 24 to 48 h after treatment with N-nitroso-N-ethylurea (NEU; 50 mg/kg), DXR (5 mg/kg), sodium chloride (NaCl; 150 mM), and THI (0.5-2 g/kg). Antigenotoxic assays were carried out using THI (0.5 g/kg) in combination with NEU or DXR. Analysis of the micronucleated polychromatic erythrocytes (MNPCEs) indicated no significant differences between treatment doses of THI (0.5-2 g/kg) and NaCl. Polychromatic erythrocyte (PCE) to normochromatic erythrocyte (NCE) ratios did not indicate any statistical differences between DXR and THI or NaCl, but there were differences between THI and NaCl. A significant reduction in MNPCEs and PCE/NCE ratios was observed when THI was administered in combination with DXR. This study suggested the absence of THI genotoxicity that was dose-, time-, and gender-independent and the presence of moderate systemic toxicity that was dose-independent, but time- and gender-dependent. The combination of THI and DXR also suggested antigenotoxic effects, indicating that THI reduced genotoxic effects induced by chemotherapeutic agents.(AU)

Handroanthus impetiginosus tem sido usada durante um longo período pela medicina tradicional e vários estudos têm demonstrados a presença de compostos químicos e potencial fitoterapêutico. Esta pesquisa avaliou a genotoxicidade da tintura da casca liofilizada de H. impetiginosus (THI) usando o ensaio do micronúcleo em medula óssea de camundongos. A interação entre THI e os efeitos genotóxicos induzidos pelo quimioterápico doxorrubicina (DXR) também foram analisados. Grupos experimentais foram analisados a 24-48 h após o tratamento com N-Nitroso-N-etiluréia (NEU; 50 mg/kg), DXR (5 mg/kg), NaCl (150 mM) e THI (0,5-2 g/kg). O ensaio antigenotóxico foi conduzido utilizando THI (0,5 g/kg) em combinação com NEU ou DXR. A análise de eritrócitos policromáticos micronucleados (EPCMNs) não mostrou diferenças significativas entre as doses de tratamento (0,5-2 g/kg) e NaCl. As proporções de eritrócitos policromáticos (EPC)/eritrócitos normocromáticos (ENC) não revelaram diferenças estatísticas entre DXR e THI ou NaCl, porém houve diferenças entre THI e NaCl. Uma redução significativa em EPCMNs e na razão EPC/ENC foi observada quando THI foi administrado em combinação com DXR. Essa pesquisa sugere ausência de genotoxicidade de THI, dose-, tempo- e sexo-independente, e moderada toxicidade sistêmica dose-independente, mas tempo- e sexo-dependente. A associação do THI e DXR também sugere efeitos antigenotóxicos. Por conseguinte, THI pode reduzir os efeitos genotóxicos induzidos pelo quimioterapêutico.(AU)

Bem-estar e qualidade de ovos de codornas alimentadas com extratos de camomila e passiflora / Welfare and quality of quail eggs fed with extracts of chamomile and passionflower

The objective of this study was to evaluate the effect of different combinations of passionflower (Passiflora alata) and chamomile (Matricaria chamomile) on performance, egg quality, behavior and stress of quails during the rearing and laying phases. A total of 196 birds was distributed in randomized blocks, divided into four treatments (conventional feed: 125 mg of Matricaria chamomile + 375 mg of Passiflora alata/kg of feed; 250 mg of M. chamomile + 250 mg of P. alata/kg of feed; 375 mg of M. chamomile + 125 mg P. alata/kg of feed), with seven replications and seven birds per cage. The rearing period began at 28 days of age and lasted 14 days. At this stage were evaluated daily feed intake, daily weight gain, feed conversion, viability, time in tonic immobility (TIT), intensity of injury and heterophil/lymphocyte ratio (H:L). The laying period started when quails reached 42 days of age, in which feed intake, feed conversion, egg production and quality, viability, TIT, intensity of injury and H:L ratio. In the rearing period, the inclusion of the phytotherapics in the diets did not alter the performance, H:L ratio and the intensity of injury (P≥0,05), but the TIT decreased when adding 250 mg of each phytotherapic (P<0,05). In the laying period, the different combinations of chamomile and passionflower did not affect the performance and external quality of fresh eggs (P≥0,05). However, when 250 mg of each phytotherapic was added to the diets, there was a reduction in TIT, H:L ratio and intensity of injury in the head and body of the quails (P<0,05), indicating that the birds were less stressed. As for the internal quality of fresh eggs, there was an improvement in the Haugh unit and the percentages of yolk and albumen when adding 250 mg of each phytotherapic (P<0,05). We concluded that the inclusion of chamomile and passionflower in the diets does not alter the performance of the quails during the rearing and laying periods, does not influence the external...

O objetivo deste estudo foi avaliar o efeito de diferentes combinações de passiflora (Passiflora alata) e de camomila (Matricaria camomila) sobre o desempenho, qualidade de ovos, comportamento e estresse de codornas durante as fases de recria e de postura. Foram utilizadas 196 aves, distribuídas em blocos ao acaso, com quatro tratamentos (ração convencional; 125 mg de Matricaria chamomila + 375 mg de Passiflora alata/kg de ração; 250 mg de M. chamomila + 250 mg de P. alata/kg de ração; 375 mg de M. chamomila + 125 mg P. alata/kg de ração), com sete repetições e sete aves por parcela. A fase de recria iniciou-se aos 28 dias de idade das aves, durando 14 dias. Nesta fase foram avaliados consumo diário de ração, ganho diário de peso, conversão alimentar, viabilidade de criação, o tempo em imobilidade tônica (TIT), a intensidade de ferimentos e a relação entre heterófilos e linfócitos (H:L). A fase de postura teve início quando as aves atingiram 42 dias de idade, em que foram avaliados consumo de ração, conversão alimentar, produção e qualidade de ovos, viabilidade, TIT, intensidade de ferimentos e relação H:L. Na fase de recria a inclusão dos fitoterápicos às rações não alterou o desempenho, a relação H:L e as intensidades de lesões (P≥0,05), mas diminuiu o TIT quando da adição de 250 mg de cada fitoterápico (P<0,05). Na fase de postura as diferentes combinações de camomila e passiflora não afetaram o desempenho e a qualidade externa de ovos frescos (P≥0,05). No entanto, quando se adicionou 250 mg de cada extrato nas rações houve redução no TIT, na relação H:L e na intensidade de lesões na cabeça e corpo das aves (P<0,05), indicando que as aves estavam menos estressadas. Quanto à qualidade interna de ovos frescos, verificou-se melhora na unidade Haugh e nas porcentagens de gema e de albúmen quando da inclusão de 250 mg de cada fitoterápico (P<0,05). Conclui-se que a adição de camomila e de passiflora nas rações não altera o desempenho produtivo das aves nas...

Induced aerocystitis and hemato-immunological parameters in Nile tilapia fed supplemented diet with essential oil of Lippia alba / Aerocistite induzida e parâmetros hemato-imunológicos em tilápia do Nilo suplementada com óleo essencial de Lippia alba

The present study evaluated the dietary supplementation with essential oil of Lippia alba on the hemato-immunological parameters of Nile tilapia (Oreochromis niloticus) submitted to acute inflammation induced by carrageenin injection in the swim bladder. For a period of 45 days, 96 fish were divided in four treatments in triplicate, as follows: fish fed supplemented diet with essential oil of L. alba (4 mL kg-1 dry ration) injected with carrageenin; fish fed supplemented diet with cereal alcohol injected with carrageenin; fish fed unsupplemented diet with essential oil injected with carrageenin; fish fed unsupplemented diet and noninjected. Cortisol levels, erythrogram, leukogram and the inflammatory infiltrate were analyzed 6 h after inflammatory stimulus. Carrageenin-injected fish showed acute inflammatory reaction in the swim bladder characterized by higher infiltrate of neutrophils and monocytes. The circulating neutrophils number was significantly higher in fish fed L. alba when compared to other treatments. No difference in cortisol levels was found. For dose, time and administration form tested, supplementation with essential oil of L. alba did not present anti-inflammatory activity. On the other hand, influence of dietary supplementation was observed on the neutrophils number after induced aerocystitis highlighting its immunomodulatory characteristic.(AU)

O presente estudo avaliou a suplementação dietária com óleo essencial de Lippia alba sobre os parâmetros hemato-imunológicos em tilápias do Nilo (Oreochromis niloticus) submetidas à inflamação aguda induzida por carragenina na bexiga natatória. Pelo período de 45 dias, 96 peixes foram divididos em quarto tratamentos em triplicata: a) peixes suplementados com óleo esencial de L. alba (4 mL kg-1 de ração) injetados com carragenina; b) peixes suplementados com álcool de cereais injetados com carragenina; peixes não suplementados com óleo essencial injetados com carragenina; c) peixes não suplementados não injetados. Os níveis de cortisol, eritrograma, leucograma e o infiltrado inflamatório foram analisados seis horas após o estímulo inflamatório. Peixes injetados com carragenina apresentaram reação inflamatória aguda na bexiga natatória caracterizada por maior infiltrado de neutrófilos e monócitos. O número de neutrófilos circulantes foi significativamente maior nos peixes suplementados com L. alba quando comparado aos outros tratamentos. Não houve diferença nos níveis de cortisol. Para a dose, o tempo e a forma de administração testada, a suplementação com óleo essencil de L. alba não apresentou atividade anti-inflamatória. Por outro lado, foi constatada influência da suplementação dietária no número de neutrófilos após a aerocistite enfatizando a sua característica imunomoduladora.(AU)