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1.
PLoS One ; 19(9): e0309946, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39241030

RESUMO

Comparative studies reliant on single personality surveys to rate wild primates are scarce yet remain critical for developing a holistic comparative understanding of personality. Differences in survey design, item exclusion, and factor selection impede cross-study comparisons. To address these challenges, we used consistently collected data to assess personality trait structures in wild rhesus (Macaca mulatta), bonnet (M. radiata), and long-tailed (M. fascicularis) macaques that varied in their degree of phylogenetic closeness, species-typical social styles, and anthropogenic exposure in urban or urban-rural environments. We administered 51-item personality surveys to familiar raters, and, after reliability and structure screenings, isolated 4-5 factor solutions among the species. Four consistent factors emerged: Confident, Sociable, Active, and Irritable/Equable. This latter factor had differential expression across species. Item composition of the Irritable/Equable factor was consistent with their anticipated differences in social styles, but confounded by cross-site anthropogenic variation. We also administered a 43-item survey confined to human-primate situations which paralleled our findings of social style variation, while also exhibiting variation that aligned with population differences in human density. Our findings indicate that macaque personality trait structures may be emergent outcomes of evolutionary and/or socioecological processes, but further research is needed to parse these processes' relative contributions.


Assuntos
Personalidade , Animais , Personalidade/fisiologia , Humanos , Masculino , Feminino , Especificidade da Espécie , Macaca/fisiologia , Comportamento Animal/fisiologia , Comportamento Social , Macaca mulatta
2.
Cereb Cortex ; 34(9)2024 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-39227311

RESUMO

The present study aimed to describe the cortical connectivity of a sector located in the ventral bank of the superior temporal sulcus in the macaque (intermediate area TEa and TEm [TEa/m]), which appears to represent the major source of output of the ventral visual stream outside the temporal lobe. The retrograde tracer wheat germ agglutinin was injected in the intermediate TEa/m in four macaque monkeys. The results showed that 58-78% of labeled cells were located within ventral visual stream areas other than the TE complex. Outside the ventral visual stream, there were connections with the memory-related medial temporal area 36 and the parahippocampal cortex, orbitofrontal areas involved in encoding subjective values of stimuli for action selection, and eye- or hand-movement related parietal (LIP, AIP, and SII), prefrontal (12r, 45A, and 45B) areas, and a hand-related dysgranular insula field. Altogether these data provide a solid substrate for the engagement of the ventral visual stream in large scale cortical networks for skeletomotor or oculomotor control. Accordingly, the role of the ventral visual stream could go beyond pure perceptual processes and could be also finalized to the neural mechanisms underlying the control of voluntary motor behavior.


Assuntos
Vias Visuais , Animais , Masculino , Vias Visuais/fisiologia , Lobo Temporal/fisiologia , Macaca mulatta , Mapeamento Encefálico , Feminino , Desempenho Psicomotor/fisiologia , Atividade Motora/fisiologia
3.
Curr Protoc ; 4(9): e70015, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39283005

RESUMO

Primary human hepatocytes (PHHs) are recognized as the "gold standard" for evaluating toxicity of various drugs or chemicals in vitro. However, due to their limited availability, primary hepatocytes isolated from rodents are more commonly used in various experimental studies than PHHs. However, bigger differences in drug metabolism were seen between humans and rats compared to those between human and non-human primates. Here, we describe a method to isolate primary hepatocytes from the liver of rhesus macaques (Macaca mulatta, a species of Old-World monkey) after in situ whole liver perfusion. Techniques for cryopreserving and recovering primary macaque hepatocytes (PMHs) are also described. Given the remarkable physiological and genetic similarity of non-human primates to humans, PMHs isolated using this protocol may serve as a reliable surrogate of PHHs in toxicological research and preclinical studies. Published 2024. This article is a U.S. Government work and is in the public domain in the USA. Basic Protocol 1: In situ whole liver perfusion Basic Protocol 2: Primary macaque hepatocyte isolation and cell plating Basic Protocol 3: Cryopreservation and recovery of primary macaque hepatocytes.


Assuntos
Criopreservação , Hepatócitos , Macaca mulatta , Animais , Hepatócitos/citologia , Hepatócitos/efeitos dos fármacos , Criopreservação/métodos , Separação Celular/métodos , Fígado/citologia , Perfusão/métodos , Células Cultivadas
4.
Invest Ophthalmol Vis Sci ; 65(11): 23, 2024 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-39283618

RESUMO

Purpose: Insight into the immune status of the living eye is essential as we seek to understand ocular disease and develop new treatments. The nonhuman primate (NHP) is the gold standard preclinical model for therapeutic development in ophthalmology, owing to the similar visual system and immune landscape in the NHP relative to the human. Here, we demonstrate the utility of phase-contrast adaptive optics scanning light ophthalmoscope (AOSLO) to visualize immune cell dynamics on the cellular scale, label-free in the NHP. Methods: Phase-contrast AOSLO was used to image preselected areas of retinal vasculature in five NHP eyes. Images were registered to correct for eye motion, temporally averaged, and analyzed for immune cell activity. Cell counts, dimensions, velocities, and frequency per vessel were determined manually and compared between retinal arterioles and venules. Based on cell appearance and circularity index, cells were divided into three morphologies: ovoid, semicircular, and flattened. Results: Immune cells were observed migrating along vascular endothelium with and against blood flow. Cell velocity did not significantly differ between morphology or vessel type and was independent of blow flood. Venules had a significantly higher cell frequency than arterioles. A higher proportion of cells resembled "flattened" morphology in arterioles. Based on cell speeds, morphologies, and behaviors, we identified these cells as nonclassical patrolling monocytes (NCPMs). Conclusions: Phase-contrast AOSLO has the potential to reveal the once hidden behaviors of single immune cells in retinal circulation and can do so without the requirement of added contrast agents that may disrupt immune cell behavior.


Assuntos
Macaca mulatta , Vasos Retinianos , Animais , Masculino , Oftalmoscopia/métodos , Movimento Celular/fisiologia , Vênulas , Arteríolas , Microscopia de Contraste de Fase , Oftalmoscópios , Macaca fascicularis
5.
Nat Commun ; 15(1): 7964, 2024 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-39261491

RESUMO

Fixational eye movements alter the number and timing of spikes transmitted from the retina to the brain, but whether these changes enhance or degrade the retinal signal is unclear. To quantify this, we developed a Bayesian method for reconstructing natural images from the recorded spikes of hundreds of retinal ganglion cells (RGCs) in the macaque retina (male), combining a likelihood model for RGC light responses with the natural image prior implicitly embedded in an artificial neural network optimized for denoising. The method matched or surpassed the performance of previous reconstruction algorithms, and provides an interpretable framework for characterizing the retinal signal. Reconstructions were improved with artificial stimulus jitter that emulated fixational eye movements, even when the eye movement trajectory was assumed to be unknown and had to be inferred from retinal spikes. Reconstructions were degraded by small artificial perturbations of spike times, revealing more precise temporal encoding than suggested by previous studies. Finally, reconstructions were substantially degraded when derived from a model that ignored cell-to-cell interactions, indicating the importance of stimulus-evoked correlations. Thus, fixational eye movements enhance the precision of the retinal representation.


Assuntos
Movimentos Oculares , Fixação Ocular , Retina , Células Ganglionares da Retina , Animais , Células Ganglionares da Retina/fisiologia , Retina/fisiologia , Movimentos Oculares/fisiologia , Masculino , Fixação Ocular/fisiologia , Macaca mulatta , Teorema de Bayes , Algoritmos , Potenciais de Ação/fisiologia , Estimulação Luminosa , Modelos Neurológicos
6.
Alcohol Alcohol ; 59(5)2024 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-39233472

RESUMO

AIMS: As the interactions of alcohol and HIV/SIV infection and their impact on liver metabolic homeostasis remain to be fully elucidated, this study aimed to determine alcohol-mediated hepatic adaptations of metabolic pathways in SIV/ART-treated female rhesus macaques fed a nutritionally balanced diet. METHODS: Macaques were administered chronic binge alcohol (CBA; 13-14 g ethanol/kg/week for 14.5 months; n = 7) or vehicle (VEH; n = 8) for 14.5 months. Livers were excised following an overnight fast. Gene and protein expression, enzymatic activity, and lipid content were determined using frozen tissue and histological staining was performed using paraffin-embedded tissue. RESULTS: CBA/SIV macaques showed increased hepatic protein expression of electron transport Complex III and increased gene expression of glycolytic (phosphofructokinase and aldolase) and gluconeogenic (pyruvate carboxylase) enzymes and of genes involved in lipid turnover homeostasis (perilipin 1, peroxisome proliferator-activated receptor gamma, carbohydrate responsive binding protein, and acetyl-CoA carboxylase B) as compared to that of livers from the VEH/SIV group. Plasma triglyceride concentration had a significant positive association with liver triglyceride content in the CBA/SIV group. CONCLUSIONS: These results reflect CBA-associated alterations in expression of proteins and genes involved in glucose and lipid metabolism homeostasis without significant evidence of steatosis or dysglycemia. Whether these changes predispose to greater liver pathology upon consumption of a high fat/high sugar diet that is more aligned with dietary intake of PWH and/or exposure to additional environmental factors warrants further investigation.


Assuntos
Consumo Excessivo de Bebidas Alcoólicas , Fígado , Macaca mulatta , Síndrome de Imunodeficiência Adquirida dos Símios , Animais , Feminino , Síndrome de Imunodeficiência Adquirida dos Símios/metabolismo , Fígado/metabolismo , Fígado/efeitos dos fármacos , Consumo Excessivo de Bebidas Alcoólicas/metabolismo , Adaptação Fisiológica/efeitos dos fármacos , Etanol/farmacologia , Metabolismo dos Lipídeos/efeitos dos fármacos
7.
PLoS Pathog ; 20(9): e1012477, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39226323

RESUMO

The most commonly used animal models for evaluating the efficacy of HSV-2 candidate vaccines are mice and guinea pigs. While numerous HSV-2 vaccine candidates have been tested in these animals and were effective in reducing disease and mortality, these results did not predict the effectiveness of the vaccines in human trials. Infection of rhesus macaques rarely results in lesions or HSV-2 specific antibody responses. In seeking an animal model that better recapitulates human disease and that might be more predictive of the efficacy of prophylactic vaccines than mice and guinea pigs, we evaluated Cebus apella (C. apella), a New World primate, in an HSV-2 genital infection model. Infectious HSV-2 was cultured from vaginal swabs from all 4 animals for 9-14 days after intravaginal inoculation of HSV-2 seronegative monkeys. Two of 4 monkeys had vesicular lesions in the vagina or vulva. No neurological symptoms were noted. Recurrent lesions and HSV-2 DNA shedding after acute disease resolved was infrequent. UV irradiation of the genital area did not induce recurrent genital lesions or virus shedding. All 4 monkeys developed HSV-2 neutralizing antibodies as well as virus-specific CD4 and CD8 T cell responses. Reinfection of animals 15 to 19 months after primary infection did not result in lesions; animals had reduced virus shedding and a shorter duration of shedding compared with that during primary infection, suggesting that primary infection induced protective immunity. Primary fibroblasts from C. apella monkeys supported the growth of HSV-2 in vitro; in contrast, HSV-2 did not replicate above the titer of the input inoculum in fibroblasts from rhesus macaques. These observations suggest that the C. apella monkey has potential to serve as a model for evaluating the efficacy of prophylactic vaccines, antivirals, or monoclonal antibodies to HSV-2.


Assuntos
Modelos Animais de Doenças , Herpes Genital , Herpesvirus Humano 2 , Soroconversão , Eliminação de Partículas Virais , Animais , Herpes Genital/imunologia , Herpes Genital/virologia , Feminino , Herpesvirus Humano 2/imunologia , Eliminação de Partículas Virais/imunologia , Anticorpos Antivirais/imunologia , Vagina/virologia , Vagina/imunologia , Vagina/patologia , Macaca mulatta
8.
Commun Biol ; 7(1): 1080, 2024 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-39227400

RESUMO

Nonhuman primates (NHPs) exhibit complex and diverse behavior that typifies advanced cognitive function and social communication, but quantitative and systematical measure of this natural nonverbal processing has been a technical challenge. Specifically, a method is required to automatically segment time series of behavior into elemental motion motifs, much like finding meaningful words in character strings. Here, we propose a solution called SyntacticMotionParser (SMP), a general-purpose unsupervised behavior parsing algorithm using a nonparametric Bayesian model. Using three-dimensional posture-tracking data from NHPs, SMP automatically outputs an optimized sequence of latent motion motifs classified into the most likely number of states. When applied to behavioral datasets from common marmosets and rhesus monkeys, SMP outperformed conventional posture-clustering models and detected a set of behavioral ethograms from publicly available data. SMP also quantified and visualized the behavioral effects of chemogenetic neural manipulations. SMP thus has the potential to dramatically improve our understanding of natural NHP behavior in a variety of contexts.


Assuntos
Teorema de Bayes , Comportamento Animal , Macaca mulatta , Animais , Algoritmos , Callithrix/fisiologia , Masculino
9.
Mol Brain ; 17(1): 65, 2024 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-39227961

RESUMO

It is a consensus in the international manned space field that factors such as microgravity during the space flight can cause anxiety, depression and other important brain function abnormalities in astronauts. However, the neural mechanism at the molecular level is still unclear. Due to the limitations of research conditions, studies of biological changes in the primate brain have been comparatively few. We took advantage of -6° head-down bed rest (HDBR), one of the most implemented space analogues on the ground, to investigate the effects of simulated weightlessness on non-human primate brain metabolites. The Rhesus Macaque monkeys in the experiment were divided into three groups: the control group, the 42-day simulated weightlessness group with HDBR, and the recovery group, which had 28 days of free activity in the home cage after the HDBR. Liquid chromatography-mass spectrometry (LC-MS) was used to perform metabolomics analysis on specific brain areas of the monkeys under three experimental conditions. Our results show that simulated weightlessness can cause neurotransmitter imbalances, the amino acid and energy metabolism disorders, and hormone disturbances. But these metabolomics changes are reversible after recovery. Our study suggests that long-term brain damage in space flight might be reversible at the metabolic level. This lays a technical foundation for ensuring brain health and enhancing the brain function in future space studies.


Assuntos
Repouso em Cama , Encéfalo , Decúbito Inclinado com Rebaixamento da Cabeça , Macaca mulatta , Simulação de Ausência de Peso , Animais , Encéfalo/metabolismo , Masculino , Metabolômica , Ausência de Peso/efeitos adversos , Neurotransmissores/metabolismo , Aminoácidos/metabolismo , Hormônios/metabolismo
10.
J Med Primatol ; 53(5): e12733, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39245886

RESUMO

An adult female rhesus macaque presented during routine annual physical examination for evaluation of a 2.5-cm diameter superficial ulcerated dermal lesion that was subsequently diagnosed as a systemic fungal infection caused by Cryptococcus gattii. Cryptococcus gattii is one of several basidiomycetic yeasts responsible for pulmonary, neurologic, and disseminated infections in humans and animals. This report describes the diagnosis, management, and clinical resolution of a C. gattii infection in an immunocompetent 5-year-old female rhesus macaque.


Assuntos
Antifúngicos , Criptococose , Cryptococcus gattii , Macaca mulatta , Doenças dos Macacos , Animais , Cryptococcus gattii/isolamento & purificação , Criptococose/veterinária , Criptococose/tratamento farmacológico , Criptococose/diagnóstico , Criptococose/microbiologia , Feminino , Doenças dos Macacos/microbiologia , Doenças dos Macacos/diagnóstico , Doenças dos Macacos/tratamento farmacológico , Antifúngicos/uso terapêutico , Imunocompetência
11.
J Comp Neurol ; 532(8): e25665, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39235147

RESUMO

Astrocytes intricately weave within the neuropil, giving rise to characteristic bushy morphologies. Pioneering studies suggested that primate astrocytes are more complex due to increased branch numbers and territory size compared to rodent counterparts. However, there has been no comprehensive comparison of astrocyte morphology across species. We employed several techniques to investigate astrocyte morphology and directly compared them between mice and rhesus macaques in cortical and subcortical regions. We assessed astrocyte density, territory size, branching structure, fine morphological complexity, and interactions with neuronal synapses using a combination of techniques, including immunohistochemistry, adeno-associated virus-mediated transduction of astrocytes, diOlistics, confocal imaging, and electron microscopy. We found significant morphological similarities between primate and rodent astrocytes, suggesting that astrocyte structure has scaled with evolution. Our findings show that primate astrocytes are larger and more numerous than those in rodents but contest the view that primate astrocytes are morphologically far more complex.


Assuntos
Astrócitos , Macaca mulatta , Animais , Astrócitos/ultraestrutura , Camundongos , Camundongos Endogâmicos C57BL , Especificidade da Espécie , Masculino , Encéfalo/citologia
12.
Physiol Rep ; 12(17): e70037, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39245818

RESUMO

Prior studies have documented the role of the striatum and its dopaminergic input in time processing, but the contribution of local striatal cholinergic innervation has not been specifically investigated. To address this issue, we recorded the activity of tonically active neurons (TANs), thought to be cholinergic interneurons in the striatum, in two male macaques performing self-initiated movements after specified intervals in the seconds range have elapsed. The behavioral data showed that movement timing was adjusted according to the temporal requirements. About one-third of all recorded TANs displayed brief depressions in firing in response to the cue that indicates the interval duration, and the strength of these modulations was, in some instances, related to the timing of movement. The rewarding outcome of actions also impacted TAN activity, as reflected by stronger responses to the cue paralleled by weaker responses to reward when monkeys performed correctly timed movements over consecutive trials. It therefore appears that TAN responses may act as a start signal for keeping track of time and reward prediction could be incorporated in this signaling function. We conclude that the role of the striatal cholinergic TAN system in time processing is embedded in predicting rewarding outcomes during timing behavior.


Assuntos
Corpo Estriado , Macaca mulatta , Recompensa , Animais , Masculino , Projetos Piloto , Corpo Estriado/fisiologia , Neurônios Colinérgicos/fisiologia , Neurônios/fisiologia , Sinais (Psicologia) , Potenciais de Ação/fisiologia , Percepção do Tempo/fisiologia
13.
Int J Mol Sci ; 25(17)2024 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-39273157

RESUMO

In the last decade, geopolitical instability across the globe has increased the risk of a large-scale radiological event, when radiation biomarkers would be needed for an effective triage of an irradiated population. Ionizing radiation elicits a complex response in the proteome, genome, and metabolome and hence can be leveraged as rapid and sensitive indicators of irradiation-induced damage. We analyzed the plasma of total-body irradiated (TBI) leukemia patients (n = 24) and nonhuman primates (NHPs; n = 10) before and 24 h after irradiation, and we performed a global metabolomic study aiming to provide plasma metabolites as candidate radiation biomarkers for biological dosimetry. Peripheral blood samples were collected according to the appropriate ethical approvals, and metabolites were extracted and analyzed by liquid chromatography mass spectrometry. We identified an array of metabolites significantly altered by irradiation, including bilirubin, cholesterol, and 18-hydroxycorticosterone, which were detected in leukemia patients and NHPs. Pathway analysis showed overlapping perturbations in steroidogenesis, porphyrin metabolism, and steroid hormone biosynthesis and metabolism. Additionally, we observed dysregulation in bile acid biosynthesis and tyrosine metabolism in the TBI patient cohort. This investigation is, to our best knowledge, among the first to provide valuable insights into a comparison between human and NHP irradiation models. The findings from this study could be leveraged for translational biological dosimetry.


Assuntos
Metaboloma , Irradiação Corporal Total , Animais , Humanos , Masculino , Feminino , Adulto , Biomarcadores/sangue , Pessoa de Meia-Idade , Leucemia/sangue , Leucemia/metabolismo , Macaca mulatta , Radiação Ionizante , Metabolômica/métodos
14.
Microbiome ; 12(1): 161, 2024 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-39223641

RESUMO

BACKGROUND: Many studies have demonstrated the association between intestinal microbiota and joint diseases. The "gut-joint axis" also has potential roles in chikungunya virus (CHIKV) infection. Pro-inflammatory arthritis after CHIKV infection might disrupt host homeostasis and lead to dysbacteriosis. This study investigated the characteristics of fecal and gut microbiota, intestinal metabolites, and the changes in gene regulation of intestinal tissues after CHIKV infection using multi-omics analysis to explore the involvement of gut microbiota in the pathogenesis of CHIKV infection. RESULTS: CHIKV infection increases the systemic burden of inflammation in the GI system of infected animals. Moreover, infection-induced alterations in GI microbiota and metabolites may be indirectly involved in the modulation of GI and bone inflammation after CHIKV infection, including the modulation of inflammasomes and interleukin-17 inflammatory cytokine levels. CONCLUSION: Our results suggest that the GI tract and its microbes are involved in the modulation of CHIKV infection, which could serve as an indicator for the adjuvant treatment of CHIKV infection. Video Abstract.


Assuntos
Febre de Chikungunya , Vírus Chikungunya , Fezes , Microbioma Gastrointestinal , Macaca mulatta , Animais , Fezes/microbiologia , Febre de Chikungunya/virologia , Bactérias/classificação , Bactérias/metabolismo , Bactérias/isolamento & purificação , Bactérias/genética , Disbiose/microbiologia , Inflamação , Inflamassomos/metabolismo , Modelos Animais de Doenças , Interleucina-17/metabolismo , Trato Gastrointestinal/microbiologia , Citocinas/metabolismo
15.
Bull Exp Biol Med ; 177(3): 379-382, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-39134810

RESUMO

Variable number tandem repeat (VNTR) polymorphisms of the human neonatal IgG Fc receptor α-chain gene (FCGRT) are known to influence the expression levels of FCGRT and IgG in serum. Monkeys are considered to be a relevant biological model for studying the effects of immunobiological drugs. The study determined the functional VNTR polymorphisms of the FCGRT gene in 109 male rhesus macaques from the nursery of the Kurchatov Complex of Medical Primatology. PCR amplification of samples was carried out followed by electrophoretic separation of DNA fragments in a 2% agarose gel. Individual DNA amplification products were sequenced (according to Sanger system) in forward and reverse directions to confirm the specificity. The genotyping showed that the VNTR polymorphism of the FCGRT gene in the studied population of rhesus macaques is presented by 9 variants. The frequency of the VNTR5 allele associated with lower IgG levels was 14.2%, and the most common one was the VNTR7 allele (25.2%). We also identified alleles that have not been previously reported: VNTR3, VNTR4, VNTR6, VNTR8, and VNTR9. The study allows to consider rhesus macaques as a potential model for studying the immunological response depending on the genetic VNTR variant of FCGRT.


Assuntos
Alelos , Macaca mulatta , Repetições Minissatélites , Polimorfismo Genético , Animais , Macaca mulatta/genética , Repetições Minissatélites/genética , Polimorfismo Genético/genética , Masculino , Frequência do Gene/genética , Imunoglobulina G/sangue , Imunoglobulina G/genética , Receptores Fc/genética , Genótipo , Antígenos de Histocompatibilidade Classe I
16.
Invest Ophthalmol Vis Sci ; 65(10): 44, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-39207298

RESUMO

Purpose: The purpose of this study was to determine the effects of axial elongation on optic nerve head morphology and macula inner retinal thickness in young rhesus monkeys. Methods: Both eyes of 26 anisometropic, 1-year-old rhesus monkeys were imaged using optical coherence tomography (OCT). Before imaging, the animals were sedated, their eyes were dilated, and axial length was measured using an optical biometer. OCT imaging included a 20 degrees, 24-line radial scan centered on the optic nerve head (ONH) and two 20 degrees × 20 degrees raster scans, one centered on the ONH and the other on the macula. Radial scans were analyzed using programs written in MATLAB to quantify the Bruch's membrane opening (BMO) area and position, minimum rim width (MRW), anterior lamina cribrosa surface (ALCS) position, size of any scleral crescent, circumpapillary retinal nerve fiber layer (RNFL), and choroid thickness (pCh). Macula total retinal thickness (mTRT) and ganglion cell inner plexiform layer (GCIPL) thicknesses were quantified from macula scans. Linear least square regression was determined for OCT measures and axial length of the right eye, and for inter-eye differences. Results: Animals were 341 ± 18 days old at the time of imaging. BMO area (R2 = 0.38), ALCS position (R2 = 0.45), scleral crescent area (R2 = 0.35), pCh thickness (R2 = 0.21), mTRT (R2 = 0.24), and GCIPL thickness (R2 = 0.16) were correlated with the axial length (all P < 0.05). For each of these parameters, the right-eye regression slope did not differ from the slope of the interocular difference (P > 0.57). Conclusions: There are posterior segment morphological differences in anisometropic rhesus monkeys related to axial length. Whether these differences increase the risk of pathology remains to be investigated.


Assuntos
Macaca mulatta , Disco Óptico , Células Ganglionares da Retina , Tomografia de Coerência Óptica , Animais , Tomografia de Coerência Óptica/métodos , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/patologia , Disco Óptico/anatomia & histologia , Disco Óptico/diagnóstico por imagem , Macula Lutea/diagnóstico por imagem , Macula Lutea/anatomia & histologia , Fibras Nervosas/patologia , Comprimento Axial do Olho/anatomia & histologia , Comprimento Axial do Olho/diagnóstico por imagem , Modelos Animais de Doenças , Masculino
17.
PLoS One ; 19(8): e0307906, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39197026

RESUMO

Temporal lobe epilepsy (TLE) is the most common form of drug-resistant epilepsy. A major focus of human and animal studies on TLE network has been the limbic circuit. However, there is also evidence suggesting an active role of the basal ganglia in the propagation and control of temporal lobe seizures. Here, we characterize the involvement of the substantia nigra (SN) and somatosensory cortex (SI) during temporal lobe (TL) seizures induced by penicillin injection in the hippocampus (HPC) of two nonhuman primates. The seizure onset and offset patterns were manually classified and spectral power and coherence were calculated. We then compared the 3-second segments recorded in pre-ictal, onset, offset and post-ictal periods based on the seizure onset and offset patterns. Our results demonstrated an involvement of the SN and SI dependent on the seizure onset and offset pattern. We found that low amplitude fast activity (LAF) and high amplitude slow activity (HAS) onset patterns were associated with an increase in activity of the SN while the change in activity was limited to LAF seizures in the SI. However, the increase in HPC/SN coherence was specific to the farther-spreading LAF onset pattern. As for the role of the SN in seizure cessation, we observed that the coherence between the HPC/SN was reduced during burst suppression (BS) compared to other termination phases. Additionally, we found that this coherence returned to normal levels after the seizure ended, with no significant difference in post-ictal periods among the three types of seizure offsets. This study constitutes the first demonstration of TL seizures entraining the SN in the primate brain. Moreover, these findings provide evidence that this entrainment is dependent on the onset and offset pattern and support the hypothesis that the SN might play a role in the maintenance and termination of some specific temporal lobe seizure.


Assuntos
Modelos Animais de Doenças , Epilepsia do Lobo Temporal , Convulsões , Substância Negra , Animais , Epilepsia do Lobo Temporal/fisiopatologia , Epilepsia do Lobo Temporal/induzido quimicamente , Substância Negra/fisiopatologia , Convulsões/fisiopatologia , Masculino , Eletroencefalografia , Córtex Somatossensorial/fisiopatologia , Lobo Temporal/fisiopatologia , Hipocampo/fisiopatologia , Macaca mulatta
18.
Nat Commun ; 15(1): 6487, 2024 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-39198415

RESUMO

Primates must adapt to changing environments by optimizing their behavior to make beneficial choices. At the core of adaptive behavior is the orbitofrontal cortex (OFC) of the brain, which updates choice value through direct experience or knowledge-based inference. Here, we identify distinct neural circuitry underlying these two separate abilities. We designed two behavioral tasks in which two male macaque monkeys updated the values of certain items, either by directly experiencing changes in stimulus-reward associations, or by inferring the value of unexperienced items based on the task's rules. Chemogenetic silencing of bilateral OFC combined with mathematical model-fitting analysis revealed that monkey OFC is involved in updating item value based on both experience and inference. In vivo imaging of chemogenetic receptors by positron emission tomography allowed us to map projections from the OFC to the rostromedial caudate nucleus (rmCD) and the medial part of the mediodorsal thalamus (MDm). Chemogenetic silencing of the OFC-rmCD pathway impaired experience-based value updating, while silencing the OFC-MDm pathway impaired inference-based value updating. Our results thus demonstrate dissociable contributions of distinct OFC projections to different behavioral strategies, and provide new insights into the neural basis of value-based adaptive decision-making in primates.


Assuntos
Córtex Pré-Frontal , Animais , Masculino , Córtex Pré-Frontal/fisiologia , Córtex Pré-Frontal/diagnóstico por imagem , Comportamento Animal/fisiologia , Adaptação Psicológica/fisiologia , Núcleo Caudado/fisiologia , Núcleo Caudado/diagnóstico por imagem , Recompensa , Tomografia por Emissão de Pósitrons , Macaca mulatta , Vias Neurais/fisiologia , Comportamento de Escolha/fisiologia , Tomada de Decisões/fisiologia , Tálamo/fisiologia , Tálamo/diagnóstico por imagem , Mapeamento Encefálico/métodos
19.
Hum Vaccin Immunother ; 20(1): 2374147, 2024 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-39090779

RESUMO

Entamoeba histolytica, the causative agent of amebiasis, is one of the top three parasitic causes of mortality worldwide. However, no vaccine exists against amebiasis. Using a lead candidate vaccine containing the LecA fragment of Gal-lectin and GLA-3M-052 liposome adjuvant, we immunized rhesus macaques via intranasal or intramuscular routes. The vaccine elicited high-avidity functional humoral responses as seen by the inhibition of amebic attachment to mammalian target cells by plasma and stool antibodies. Importantly, antigen-specific IFN-γ-secreting peripheral blood mononuclear cells (PBMCs) and IgG/IgA memory B cells (BMEM) were detected in immunized animals. Furthermore, antigen-specific antibody and cellular responses were maintained for at least 8 months after the final immunization as observed by robust LecA-specific BMEM as well as IFN-γ+ PBMC responses. Overall, both intranasal and intramuscular immunizations elicited a durable and functional response in systemic and mucosal compartments, which supports advancing the LecA+GLA-3M-052 liposome vaccine candidate to clinical testing.


Assuntos
Administração Intranasal , Anticorpos Antiprotozoários , Entamoeba histolytica , Entamebíase , Interferon gama , Leucócitos Mononucleares , Lipossomos , Macaca mulatta , Vacinas Protozoárias , Animais , Entamoeba histolytica/imunologia , Lipossomos/imunologia , Lipossomos/administração & dosagem , Vacinas Protozoárias/imunologia , Vacinas Protozoárias/administração & dosagem , Anticorpos Antiprotozoários/sangue , Anticorpos Antiprotozoários/imunologia , Leucócitos Mononucleares/imunologia , Entamebíase/prevenção & controle , Entamebíase/imunologia , Interferon gama/imunologia , Interferon gama/metabolismo , Injeções Intramusculares , Imunogenicidade da Vacina , Adjuvantes de Vacinas/administração & dosagem , Adjuvantes Imunológicos/administração & dosagem , Linfócitos B/imunologia , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Imunoglobulina A/imunologia , Imunoglobulina A/sangue , Antígenos de Protozoários/imunologia , Imunidade Humoral , Memória Imunológica , Proteínas de Protozoários/imunologia
20.
Proc Natl Acad Sci U S A ; 121(34): e2321659121, 2024 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-39116178

RESUMO

The primary motor cortex does not uniquely or directly produce alpha motoneurone (α-MN) drive to muscles during voluntary movement. Rather, α-MN drive emerges from the synthesis and competition among excitatory and inhibitory inputs from multiple descending tracts, spinal interneurons, sensory inputs, and proprioceptive afferents. One such fundamental input is velocity-dependent stretch reflexes in lengthening muscles, which should be inhibited to enable voluntary movement. It remains an open question, however, the extent to which unmodulated stretch reflexes disrupt voluntary movement, and whether and how they are inhibited in limbs with numerous multiarticular muscles. We used a computational model of a Rhesus Macaque arm to simulate movements with feedforward α-MN commands only, and with added velocity-dependent stretch reflex feedback. We found that velocity-dependent stretch reflex caused movement-specific, typically large and variable disruptions to arm movements. These disruptions were greatly reduced when modulating velocity-dependent stretch reflex feedback (i) as per the commonly proposed (but yet to be clarified) idealized alpha-gamma (α-γ) coactivation or (ii) an alternative α-MN collateral projection to homonymous γ-MNs. We conclude that such α-MN collaterals are a physiologically tenable propriospinal circuit in the mammalian fusimotor system. These collaterals could still collaborate with α-γ coactivation, and the few skeletofusimotor fibers (ß-MNs) in mammals, to create a flexible fusimotor ecosystem to enable voluntary movement. By locally and automatically regulating the highly nonlinear neuro-musculo-skeletal mechanics of the limb, these collaterals could be a critical low-level enabler of learning, adaptation, and performance via higher-level brainstem, cerebellar, and cortical mechanisms.


Assuntos
Macaca mulatta , Neurônios Motores , Reflexo de Estiramento , Reflexo de Estiramento/fisiologia , Animais , Neurônios Motores/fisiologia , Movimento/fisiologia , Músculo Esquelético/fisiologia , Córtex Motor/fisiologia , Simulação por Computador , Modelos Neurológicos , Braço/fisiologia
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