Carcinosinumproduces phenotypic changes of 4T1 tumor cells in vitro

Considering that there are few published studies that specifically address the exclusive use of Carcinosinumin different potencies and, most of them focused on genotypic and clinical effects, the present study was proposed to identify possible phenotypic changes, including viability, expression of HER-2 and metastatic abilities, using 4T1 cells in vitroas a model. Carcinosinum was tested in different homeopathic potencies (12cH; 30cH; 200cH) mechanically prepared using sterile pure water. The time space between preparing the potencies and using them was 24 hours.The final dilutions were inserted into the culture medium in a volume equal to 10%, at the time of cell seeding. The same succussed vehicle used to prepare the drugs (70% ethanol) diluted 1:100 in sterile pure water was used as control. All treated cells were cultured in 25 mL flasks, with cell density of 5 x 105cells/mL. After 24 hours of treatment, cells were analyzed for apoptosis index using Annexin V kit and the Countess® system. The morphology of 4T1 cells was monitored by staining cell smears with hematoxylin-eosin and Giemsa methods. HER-2 expression was assessed by immunocytochemistry and metalloproteinase activity was assessed by zymography. The determination of the cytokine profile was performed using Cytometric Bead Array (CBA). The samples were evaluated in quadruplicate and the data were analyzed by one-way ANOVA. Carcinosinum30cH presented the highest apoptotic index and reduction of MMP-9-Pro expression; Carcinosinum200cH produced the highest positivity for HER-2 and no specific effect was seen after the treatment with Carcinosinum12cH. No change in cytokine expression was seen among treatments. We conclude that Carcinosinum30cH and 200cH can change phenotypic features important totumor development in vitro. The clinical meaning of these data deserves further investigation.

Preparation of Cancer Nosodes from specific cancer tissues

Nosodes are ultra-dilute preparations made from diseased tissues or organisms used in homeopathy since 1830, more frequently used since 1880. Carcinosin is a commonly used nosode prepared over 100 years ago from cancer tissues in the last part of the nineteenth century, of which the exact scientific information on the histopathological characterization of the source material is not available. The current therapeutic indications of Carcinosin have been largely empirical and clinically derived. In such a scenario, understanding the paramount importance of the Cancer nosode, it's revamping in the light of current scientific knowledge has been identified by the author. Specific cancerous tissue samples were sourced for nosode preparation. Identified pieces of tissue were histopathologically diagnosed, thoroughly washed, quantified and triturated with powder of Saccharum lactis to prepare decimal potencies, as per standard method suggested in the Homoeopathy Pharmacopoeia of India. Centesimal potencies were prepared using electromechanical potentizer, documenting the force parameters. The Cancer nosodes from specific cancer tissues were prepared in a systematic manner, which could be used for research as well as in clinical practice. The author has attempted scientific preparation of new Cancer nosodes making them available to the profession. On similar lines, more of cancer tissues could be explored for making other nosodes. (AU)

Transtorno de ansiedade e tratamento homeopático (relato de caso) / Anxiety disorder and homeopathic treatment (case report)

Trabalho realizado a partir do relato de caso clínico de uma jovem com diagnóstico de Transtorno de Ansiedade (Transtorno de Pânico em co-morbidade a Transtorno de Ansiedade Generalizada), os quais implicavam sofrimento emocional e significativas limitações para esta paciente. O autor propôs o tratamento e condução do caso de acordo com os fundamentos do tratamento homeopático clássico, com resultado bastante favorável. São comentados detalhes do tratamento homeopático realizado, o qual mostrou-se como alternativa eficaz ao tratamento psiquiátrico alopático convencional. Conclui citando que a abordagem homeopática se mostrou relevante ao reduzir os sintomas ansiosos gerais da paciente e trazer importante qualidade de vida e retomada da funcionalidade para a mesma. (AU)

Personalidad sobreadaptada y homeopatía / Personality sobreadaptada and homeopathy

El presente trabajo está destinado a describir una estructura de personalidad que se observa cada vez con mayor frecuencia, muy favorecida por la actual “cultura del éxito”. Quienes la presentan son personas que sostienen un excesivo ajustea la realidad exterior y a sus exigencias, disociando necesidades y posibilidades emocionales y corporales. Se establece la relación del cuadro psicopatológico con algunos remedios homeopáticos. (AU)

This paper is intended to describe a personality structure that is observed with increasing frequency, favored by the current "culture of success". Those who have are people who argue overshootingto external reality and demands decoupling emotional and physical needs and possibilities. The relationship of psychopathology with some homeopathic remedies set. (AU)

Carcinosinum x Cancerinismo

O presente trabalho tem por objetivo a revisão e o estudo do medicamento Carcinosinum, considerado um nosódio do câncer em relação ao Cancerinismo. Após o estudo foi analisada a existência de indicação “única” do medicamento para indivíduos predispostos ao Cancerinismo, ou seja, indivíduos suscetíveis ao desenvolvimento de câncer em seu estágio máximo. Para tanto foram consultadas obras de Foubister , Vijinovsky, José Laércio do Egito, Julien Haffen, Leon Vannier, Nebel, entre outros. Como resultado, as autoras observaram que Carcinosinum não é um medicamento exclusivo do Cancerinismo e concluíram que cada caso deve ser individualizado para reconhecimento de sinais e sintomas que permitirão construir a totalidade sintomática.(AU)

Aethusa cynapium / Aethusa cynapium

La petite ciguë fait partie de la famille des ciguës au côté de la grande ciguë, conium et la ciguë d'eau, cicuta virosa. Elles font partie de la famille des ombellifères ayant une... (AU)

Una historia clínica muy discutida / A very debated clinical case

Voy a referir una historia clínica de nuestra cátedra que ha dado lugar a opiniones controvertidas y las reflexiones que he sugerido a los colegas en casos clínicos como éste. (AU)