A three-gene signature and clinical outcome in pediatric acute myeloid leukemia

Background Although the 5-year survival rates in pediatric acute myeloid leukemia (AML) have improved over the last decades, there is a high relapse rate for Pediatric AML patients. Methods In the present study, we mainly combine PCA with the LASSO technique to identify prognostic markers for Pediatric AML patients coming from the NCI TARGET database. Results Three key genes (EEF1A1, RPLP2, RPL19) associated with poor prognosis of pediatric AML has been screened by both PCA and LASSO Cox regression analysis. Simultaneously, we developed a risk score model to predict the prognosis of pediatric AML, according to risk scores, the patients were divided into high‐ and low‐risk groups based on the median risk score. Kaplan–Meier survival analysis indicated that Pediatric AML patients with the high-risk group have a poorer survival rate than those with a low‐risk group (p < 0.000). The receiver operating characteristic (ROC) analysis showed that the risk model has a good performance (AUC:0.669). Moreover, the clinicopathologic correlation showed that the expression levels of three genes were related to the central nervous system (CNS) disease and chloroma. GSEA identified that those pathways including oxidative phosphorylation, apoptosis and TGFB signaling pathway were differentially enriched. Conclusion Taken together, those studies suggested that a gene panel that consists of three genes (EEF1A1, RPLP2, RPL19) may act as a potential prognostic marker (AU)

Limitación de HIF-1α con pentoxifilina en isquemia tubular renal resultado de hiperoxaluria y ESWL / Limitation of HIF-1α with pentoxifillyne on renal tubular ischemia result of hiperoxaluria and ESWL

OBJETIVO: Valorar la expresión del factor 1, subunidad α (HIF-1α) inducible por hipoxia realizando litotricia extracorpórea con ondas de choque (LEOC), e investigar los efectos de la pentoxifilina en la expresión de HIF-1α. MÉTODOS: En este estudio, se utilizaron un total de cien conejos albinos de Nueva Zelanda y se dividieron en 5 grupos, cada grupo formado por 20 conejos. Los grupos fueron divididos en subgrupos: período corto (7 días) y período largo (14 días) de acuerdo con la duración del seguimiento. Se realizaron análisis inmuno-histoquímicos usando tinción nuclear para mostrar la expresión de HIF-1α en la muestra de tejido renal del conejo. RESULTADOS: La expresión HIF -1α fue más alta en los conejos a los que se les realizó LEOC (grupo 4). El grupo hiperoxalúrico, al que se le administró pentoxifilina antes de la LEOC tuvo una expresión de HIF -1α más baja en ambos subgrupos, períodos corto y largo (grupo 5) (p < 0,05). CONCLUSIÓN: En este estudio se valoró la expresión de HIF -1α y se puso de manifiesto que la LEOC puede causar lesiones de las células renales. Nuestros resultados sugieren que la pentoxifilina como un agente regulador circulatorio puede prevenir el daño celular renal inducido por la LEOC

OBJECTIVES: To evaluate hypoxia-inducible factor 1 subunit α (HIF-1α) expression during the performance of extracorporeal shock wave lithotripsy (ESWL) and to investigate the effects of pentoxyphylline on HIF-1α expression. METHODS: One hundred New Zealand Albino rabbit were used in the study divided in 5 groups. There were 20 rabbits in each group. The groups were divided in two parts: early (7 days) and late period (14 days) according to follow up duration. Immunohistochemical analyses were performed using nuclear staining to show HIF-1α expression in rabbit renal tissue sample. RESULTS: HIF-1α expression was higher in rabbits undergoing ESWL (group 4). In the hyperoxaluria group taking pentoxyphylline before ESWL (group 5), HIF-1α expression was lower in both early and late period subgroups (p < 0.05). CONCLUSION: In this study we evaluated HIF-1α expression and showed that ESWL may cause renal cell injury. Our results suggest that pentoxyphylline, as a circulatory regulator agent, may prevent renal cell injury induced by ESWL