Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 13 de 13
Filtrar
Mais filtros










Filtros aplicados
Base de dados
Intervalo de ano de publicação
1.
Braz. j. microbiol ; 49(3): 662-667, July-Sept. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-951808

RESUMO

Abstract The effect of the intracellular microenvironment in the presence of an oxygen vector during expression of a fusion protein in Escherichia coli was studied. Three organic solutions at different concentration were chosen as oxygen vectors for fumarase expression. The addition of n-dodecane did not induce a significant change in the expression of fumarase, while the activity of fumarase increased significantly to 124% at 2.5% n-dodecane added after 9 h induction. The concentration of ATP increased sharply during the first 6 h of induction, to a value 7600% higher than that in the absence of an oxygen-vector. NAD/NADH and NADP/NADPH ratios were positively correlated with fumarase activity. n-Dodecane can be used to increase the concentration of ATP and change the energy metabolic pathway, providing sufficient energy for fumarase folding.


Assuntos
Oxigênio/metabolismo , Expressão Gênica , Alcanos/metabolismo , Escherichia coli/genética , Fumarato Hidratase/metabolismo , Oxigênio/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/química , Dobramento de Proteína , Alcanos/química , Escherichia coli/metabolismo , Fumarato Hidratase/genética , Fumarato Hidratase/química , NADP/metabolismo , NADP/química
2.
São Paulo; s.n; 2015. [88] p. ilus, tab, graf.
Tese em Português | LILACS | ID: biblio-871485

RESUMO

Introdução: O Lúpus Eritematoso Sistêmico (LES) é uma doença autoimune multissistêmica de etiologia complexa que envolve fatores ambientais, genéticos e hormonais. É caracterizada pela produção de autoanticorpos e mediadores inflamatórios, ativação e proliferação de células T autorreativas e perda da autotolerância imunológica. Em pacientes com LES, a expressão do receptor primário de ativação CD69 é aumentada e a de células T supressoras/reguladoras (Treg) CD4+CD25+FoxP3+ é reduzida. O CD69 é essencial para ativação de células T CD4 autorreativas enquanto que as células Treg são importantes na manutenção da autotolerância. Desta forma, células T tem um papel central na patogênese do LES, mas os mecanismos implicados na falência da autotolerância ainda não são elucidados, destacando a importância de estudos em modelos experimentais da doença, como o de LES-induzido por pristane. Objetivo: Quantificar células T CD4+CD69+ ativadas e Treg CD4+CD25+FoxP3+ no sangue, baço e LP de camundongos Balb/c LESinduzido por pristane no sentido de avaliar a falência de autotolerância neste modelo. Métodos: Analisamos 84 camundongos Balb/c fêmeas: 52 receberam por via intraperitoneal uma dose única de 0,5 ml de pristane e 32 a mesma dose de salina. Amostras de sangue, baço e LP dos camundongos eutanasiados foram coletadas 90, 120, 180 e 300 (T90, T120, T180 e T300) dias após a inoculação de pristane ou salina. Células mononucleares do sangue periférico (CMSP), do LP (CMLP) e esplenócitos foram obtidos por lise das hemácias seguida de lavagens com RPMI medium 1640 e centrifugação, e posteriormente criopreservadas até a avaliação por citometria de fluxo usando o aparelho Guava EasyCyteTM HT (Millipore). Para esta etapa, as células foram descongeladas, lavadas com RPMI medium 1640 e incubadas com anticorpos monoclonais dirigidos contra CD3, CD4, CD25, CD28, CD69, CTLA-4, FoxP3, CD14 e Ly6C (BD PharmingenTM). Os resultados foram expressos como média ± DP e teste de...


Introduction: Systemic Lupus Erythematosus (SLE) is multisystemic autoimmune disease with complex etiology that involves environmental, genetic and hormonal factors. Is characterized by auto-antibodies and inflammatory mediators production, autoreactive T cells activation and proliferation and loss of immunogenic autotolerance. In patients with SLE, expression of CD69 activation primary receptor is increased and the CD4+CD25+FoxP3+ suppressor/regulatory T cell (Treg) is reduced. CD69 is essential for activation of autoreactive CD4 T cells while Treg cells are important in autotolerance maintenance. In this way, T cells have a central role in the pathogenesis of SLE however, the mechanisms implied in the autotolerance failure are still not elucidated, highlighting the importance of studies in this disease's experimental models, such as pristane-induced SLE. Objective: Quantify activated CD4+CD69+ T cells and CD4+CD25+FoxP3+ Treg in blood, spleen and peritoneal lavage (PL) of Balb/c mice with pristane-induced SLE in order to evaluate autotolerance failure in this model. Methods: 84 female Balb/c mice were analyzed: 52 received a single intraperitoneal 0,5 ml dose of pristane and 32 the same dose of saline. Euthanized mice samples of blood, spleen and peritoneal lavage were collected 90, 120, 180 and 300 (T90, T120, T180 and T300) days after inoculation of pristane or saline. Mononuclear cells from peripheral blood (PBMC), PL (PLMC) and splenocytes were obtained by lysis of erythrocytes followed by washings with RPMI medium 1640 and centrifugation, subsequently criopreserved until evaluation by flow cytometry using the appliance GuavaEasyCyteTM HT (Millipore). For this step, cells were unfrozen, washed with RPMI medium 1640 and incubated with monoclonal antibodies against CD3, CD4, CD25, CD28, CD69, CTLA-4, FoxP3, CD14 and Ly6C (BD PharmingenTM). The results were expressed as mean ± SD and Mann-Whitney 11 test was used for statistical analysis, being considered...


Assuntos
Animais , Feminino , Camundongos , Alcanos , Doenças Autoimunes , Lúpus Eritematoso Sistêmico/etiologia , Camundongos , Linfócitos T Reguladores
3.
Biol. Res ; 47: 1-9, 2014. graf, tab
Artigo em Inglês | LILACS | ID: lil-710935

RESUMO

BACKGROUND: Current study has been designed to evaluate the chemical composition of essential and fixed oils from stem and leaves of Perovskia abrotanoides and antioxidant and antimicrobial activities of these oils. RESULTS: GC-MS analysis of essential oil identified 19 compounds with (E)-9-dodecenal being the major component in stem and hexadecanoic acid in leaves. In contrast, GC-MS analysis of fixed oil showed 40 constituents with α-amyrin the major component in stem and α-copaene in leaves. The antioxidant activity showed the highest value of 76.7% in essential oil from leaves in comparison with fixed oil from stem (45.9%) through inhibition of peroxidation in linoleic acid system. The antimicrobial assay tested on different microorganisms (e.g. E. coli, S. aureus, B. cereus, Nitrospira, S. epidermis, A. niger, A. flavus and C. albicans) showed the higher inhibition zone at essential oil from leaves (15.2 mm on B. cereus) as compared to fixed oil from stem (8.34 mm onS. aureus) and leaves (11.2 mm on S. aureus). CONCLUSIONS: The present study revealed the fact that essential oil analyzed from Perovskia abrotanoides stem and leaves could be a promising source of natural products with potential antioxidant and antimicrobial activities, as compared to fixed oil.


Assuntos
Anti-Infecciosos/química , Antioxidantes/química , Lamiaceae/química , Folhas de Planta/química , Óleos de Plantas/farmacologia , Caules de Planta/química , Alcanos/análise , Alcanos/farmacologia , Anti-Infecciosos/farmacologia , Antioxidantes/farmacologia , Aspergillus/efeitos dos fármacos , Bacillus cereus/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Escherichia coli/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Peroxidação de Lipídeos/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Éteres Metílicos/análise , Éteres Metílicos/farmacologia , Óleos Voláteis/química , Ácido Oleanólico/análise , Ácido Oleanólico/análogos & derivados , Ácido Oleanólico/farmacologia , Ácido Palmítico/análise , Ácido Palmítico/farmacologia , Triterpenos Pentacíclicos/análise , Triterpenos Pentacíclicos/farmacologia , Óleos de Plantas/química , Substâncias Redutoras/análise , Sesquiterpenos/análise , Sesquiterpenos/farmacologia , Staphylococcus/efeitos dos fármacos , Ácidos Esteáricos/análise , Ácidos Esteáricos/farmacologia
4.
Biol. Res ; 47: 1-6, 2014. ilus, graf, tab
Artigo em Inglês | LILACS | ID: biblio-950747

RESUMO

BACKGROUND: Tribolium castaneum (Herbst) is a harmful pest of stored grain and flour-based products in tropical and subtropical region. In the present study, rhizome of Drynaria quercifolia (J. Smith) was evaluated for pesticidal and pest repellency activities against T. castaneum, using surface film method and filter paper disc method, respectively. In addition, activity of the isolated compound 3,4-dihydroxybenzoic acid was evaluated against the pest. RESULTS: Chloroform soluble fraction of ethanol extract of rhizome of D. quercifolia showed significant pesticidal activity at doses 0.88 to 1.77 mg/cm² and significant pest repellency activity at doses 0.94 to 0.23 mg/cm². No pesticidal and pest repellency activity was found for petroleum ether, ethyl acetate and methanol soluble fractions of ethanol extract as well as for 3,4-dihydroxybenzoic acid. CONCLUSION: Considering our findings it can be concluded that chloroform soluble fraction of rhizome of D. quercifoliais useful in controlling T. castaneum of stored grain and flour-based products.


Assuntos
Animais , Praguicidas , Tribolium/efeitos dos fármacos , Controle de Pragas/métodos , Polypodiaceae/química , Rizoma/química , Hidroxibenzoatos/farmacologia , Repelentes de Insetos/farmacologia , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/química , Clorofórmio , Etanol , Metanol , Alcanos , Hidroxibenzoatos/isolamento & purificação , Dose Letal Mediana , Acetatos
5.
Mem. Inst. Oswaldo Cruz ; 108(6): 778-784, set. 2013. graf
Artigo em Inglês | LILACS | ID: lil-685498

RESUMO

The cuticular hydrocarbons of the Triatoma sordida subcomplex (Hemiptera: Reduviidae: Triatominae) were ana-lysed by gas chromatography and their structures identified by mass spectrometry. They comprised mostly n-alkanes and methyl-branched alkanes with one-four methyl substitutions. n-alkanes consisted of a homologous series from C21-C33 and represented 33-45% of the hydrocarbon fraction; n-C29 was the major component. Methyl-branched alkanes showed alkyl chains from C24-C43. High molecular weight dimethyl and trimethylalkanes (from C35-C39) represented most of the methyl-branched fraction. A few tetramethylalkanes were also detected, comprising mostly even-numbered chains. Several components such as odd-numbered 3-methylalkanes, dimethylalkanes and trimethylalkanes of C37 and C39 showed patterns of variation that allowed the differentiation of the species and populations studied. Triatoma guasayana and Triatoma patagonica showed the most distinct hydrocarbon patterns within the subcomplex. The T. sordida populations from Brazil and Argentina showed significantly different hydrocarbon profiles that posed concerns regarding the homogeneity of the species. Triatoma garciabesi had a more complex hydrocarbon pattern, but it shared some similarity with T. sordida. The quantitative and qualitative variations in the cuticular hydrocarbons may help to elucidate the relationships between species and populations of this insect group.


Assuntos
Animais , Feminino , Masculino , Hidrocarbonetos/isolamento & purificação , Lipídeos/isolamento & purificação , Triatoma/química , Análise de Variância , Alcanos/análise , Cromatografia Gasosa-Espectrometria de Massas/métodos
6.
Braz. j. microbiol ; 44(2): 639-647, 2013. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-688595

RESUMO

The petroleum-derived degrading Dietzia cinnamea strain P4 recently had its genome sequenced and annotated. This allowed employing the data on genes that are involved in the degradation of n-alkanes. To examine the physiological behavior of strain P4 in the presence of n-alkanes, the strain was grown under varying conditions of pH and temperature. D. cinnamea P4 was able to grow at pH 7.0-9.0 and at temperatures ranging from 35 ºC to 45 ºC. Experiments of gene expression by real-time quantitative RT-PCR throughout the complete growth cycle clearly indicated the induction of the regulatory gene alkU (TetR family) during early growth. During the logarithmic phase, a large increase in transcriptional levels of a lipid transporter gene was noted. Also, the expression of a gene that encodes the protein fused rubredoxin-alkane monooxygenase was enhanced. Both genes are probably under the influence of the AlkU regulator.


Assuntos
Actinomycetales/genética , Actinomycetales/metabolismo , Alcanos/metabolismo , Perfilação da Expressão Gênica , Genes Bacterianos , Hidrocarbonetos/metabolismo , Redes e Vias Metabólicas/genética , Actinomycetales/crescimento & desenvolvimento , Biotransformação , Concentração de Íons de Hidrogênio , Reação em Cadeia da Polimerase em Tempo Real , Temperatura
7.
Acta odontol. latinoam ; 23(3): 210-215, Dec. 2010. graf, tab
Artigo em Inglês | LILACS | ID: biblio-949663

RESUMO

Kinetic hydrophobic measurements were performed by confronting 40 mutans streptococci from thirty 10- to 20-year-old patients with 200 ml hexadecane (Sigma). Fourteen patients had high dental caries risk (Group A), dmft + DMFT >5 with 3 or more active caries, and 16 had low dental caries risk (Group B), dmft + DMFT <3 without active caries. Twenty bacteria from Group A and 20 bacteria from Group B were typed using De La Higuera's procedure and confirmed by API strip (bio- Merieux). From the 14 patients in Group A we obtained 12 S. mutans (8 hydrophobic/ 4 non-hydrophobic), 5 S. sobrinus (4 hydrophobic/ 1 non-hydrophobic) and 3 S. rattus (hydrophobic). From the 16 patients in Group B we obtained 11 Streptococcus mutans (10 non-hydrophobic/ 1 hydrophobic), 7 Streptococcus sobrinus (6 non-hydrophobic/ 1 hydrophobic) and 2 Streptococcus rattus (hydrophobic). Patients with high dental caries risk have a higher prevalence of hydrophobic bacteria than patients with low dental caries risk (p=0.0003). All typed S. rattus were hydrophobic.


Con el objeto de evaluar una posible relacion entre hidrofobicidad y caries dental, se estudiaron 40 cepas de Streptococcus grupo mutans provenientes de 30 pacientes de entre 10 y 20 anos, 14 pacientes con tres o mas caries activas e indice ceod mas CPOD > 5 (Grupo A) y 16 pacientes sin caries activas, con ceod mas CPOD < 3 (Grupo B). Las cepas fueron aisladas a partir de muestras de saliva en AMS-BT y tipificadas por pruebas bioquimicas y API-strep realizandose la medicion cinetica de hidrofobicidad, enfrentandolas con 200 ml de hexadecano (Sigma). En el grupo A se caracterizaron 12 cepas Streptococcus mutans: 8 hidrofobicas y 4 no hidrofobicas, 5 cepas de Streptococcus sobrinus: 4 hidrofobicas y 1 no hidrofobica y 3 cepas Streptococcus rattus hidrofobicas. En el grupo B, se caracterizaron 11 cepas de S. mutans, 10 no hidrofobicas y 1 hidrofobica, 7 cepas de S. sobrinus: 6 no hidrofobicas y 1 hidrofobica y 2 cepas de S. rattus hidrofobicas. Todos los pacientes del grupo A presentaron al menos 1 cepa hidrofobica. En las cepas aisladas de estos pacientes se demostro la existencia de una alta prevalencia de Streptococcus grupo mutans con caracteristicas hidrofobicas p=0,0003. Estos resultados indicarian la relacion entre la capacidad de adherencia a hexadecano y caries activa. Todas las cepas tipificadas como S. rattus fueron hidrofobicas independientemente del grupo de origen.


Assuntos
Adolescente , Criança , Feminino , Humanos , Masculino , Adulto Jovem , Streptococcus mutans/fisiologia , Streptococcus sobrinus/fisiologia , Suscetibilidade à Cárie Dentária/fisiologia , Streptococcus/classificação , Streptococcus/fisiologia , Aderência Bacteriana/fisiologia , Índice CPO , Cárie Dentária/microbiologia , Alcanos/química , Interações Hidrofóbicas e Hidrofílicas
8.
Rev. biol. trop ; 56(4): 1603-1611, Dec. 2008. graf, tab
Artigo em Inglês | LILACS | ID: lil-637764

RESUMO

The ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. Screening of hydrocarbon-utilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in Nigeria, Africa, resulted in the isolation of five microbial strains identified as Corynebacterium sp. DDv1, Flavobacterium sp. DDv2, Micrococcus roseus DDv3, Pseudomonas aeruginosa DDv4 and Saccharomyces cerevisae DDv5. These isolates grew readily on several hydrocarbons including hexadecane, dodecane, crude oil and petroleum fractions. Axenic cultures of the organisms utilized diesel oil (1.0 % v/v) with generation times that ranged significantly (t-test, P < 0.05) between 3.25 and 3.88 day, with concomitant production of biosurfactants. Kinetics of growth indicates that biosurfactant synthesis occurred predominantly during exponential growth phase, suggesting that the bioactive molecules are primary metabolites. Strains DDv1 and DDv4 were evidently the most metabolically active in terms of substrate utilization and biosurfactant synthesis compared to other strains with respective emulsification index of 63 and 78 %. Preliminary biochemical characterization indicates that the biosurfactants are heteropolymers consisting of lipid, protein and carbohydrate moieties. The hydrocarbon catabolic properties coupled with biosurfactant-producing capabilities is an asset that could be exploited for cleanup of oil-contaminated matrices and also in food and cosmetic industries. Rev. Biol. Trop. 56 (4): 16031611. Epub 2008 December 30.


La capacidad de los microorganismos para degradar hidrocarburos del petróleo es de gran importancia para hallar un método aceptable y ambientalmente amigable para la restauración de terrenos ambientalmente contaminados. Al investigar las capacidades de los organismos de un ecosistema de estuario que utilizan hidrocarburos y producen biosurfactantes, se produjo como resultado el aislamiento de cinco cepas microbianas identificadas como Corynebacterium sp. DDv1, Flavobacterium sp. DDv2, Micrococcus roseus DDv3, Pseudomonas aeruginosa y DDv4 Saccharomyces cerevisiae DDv5. Estas cepas crecieron fácilmente en varios hidrocarburos incluyendo hexadecanos, dodecanos, petróleo crudo y fracciones de petróleo. Los cultivos axénicos de organismos utilizaron diesel (1.0% v/v) con períodos por generación con ámbitos significativos (t-test, P <0.05) de entre 3.25 y 3.88 días, con la consiguiente producción de bio-surfactantes. La cinética del crecimiento indica que la síntesis de bio-surfactante se produjo principalmente durante la fase de crecimiento exponencial, lo que sugiere que las moléculas bioactivas son metabolitos primarios. Las cepas DDv1 y DDv4 fueron evidentemente las más metabólicamente activas en términos de utilización del sustrato y la síntesis de bio-surfactantes en comparación con otras cepas con índices respectivos de emulsificación de 63 y 78%. La caracterización bioquímica preliminar indica que los bio-surfactantes son heteropolímeros constituidos de fracciones de lípidos, proteínas y carbohidratos. Las propiedades catabólicas de los hidrocarburos, junto con las capacidades de producción de bio-surfactantes, es una ventaja que puede ser aprovechada para la limpieza de terrenos contaminados con petróleo y también en la industria alimentaria y cosmética.


Assuntos
Bactérias Gram-Negativas/metabolismo , Bactérias Gram-Positivas/metabolismo , Petróleo/metabolismo , Saccharomyces/metabolismo , Tensoativos/metabolismo , Alcanos/metabolismo , Biodegradação Ambiental , Bactérias Gram-Negativas/crescimento & desenvolvimento , Bactérias Gram-Positivas/crescimento & desenvolvimento , Nigéria , Saccharomyces/crescimento & desenvolvimento , Fatores de Tempo
9.
Electron. j. biotechnol ; 11(1): 13-25, Jan. 2008. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-522157

RESUMO

The purpose of this research was to study the effect of the methyl branching of a high log P alkane solvent and the water activity in the organic medium on the initial rate and the enantioselectivity of ibuprofen esterification catalyzed by Candida rugosa lipase. Resolution of ibuprofen is important because S-(+)-ibuprofen has the desired pharmacological activity, whereas the R-(-)-enantiomer causes much of the side effects. The Candida rugosa lipase-catalyzed reaction in isooctane at 40ºC and 0.73 water activity gave the best results, both in terms of the initial reaction rate and the enantioselectivity of the reaction. An increase in water activity allowed a higher reaction rate and enantiomeric excess in each of the four solvents. An increase in methyl branching did not necessarily increase the initial reaction rate, but it allowed a higher enantioselectivity, evidenced by an increase in the substrate enantiomeric excess.


Assuntos
Alcanos , Ibuprofeno/farmacocinética , Ibuprofeno/farmacologia , Candida/química , Lipase
10.
Clinics ; 63(6): 815-820, 2008. ilus, graf
Artigo em Inglês | LILACS | ID: lil-497896

RESUMO

OBJECTIVE: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. METHODS: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. RESULTS: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- -0.0001) than that of the control group. CONCLUSION: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.


Assuntos
Animais , Feminino , Gravidez , Ratos , Alcanos , Desenvolvimento Ósseo/efeitos dos fármacos , Cissus/química , Desenvolvimento Fetal/efeitos dos fármacos , Extratos Vegetais/farmacologia , Distribuição Aleatória , Ratos Wistar , Testes de Toxicidade Aguda
11.
An. acad. bras. ciênc ; 75(1): 33-38, Mar. 2003. graf
Artigo em Inglês | LILACS | ID: lil-331142

RESUMO

Bombardment of pyrene-doped n-heneicosane (C21H44) in its orthorhombic solid phase with <1.3> MeV broadband X-rays results in the formation of both mono- and di-heneicosylpyrenes, whereas the same dose in liquid cyclohexane yields only monosubstituted pyrene. In both cases, the reaction efficiency decreases as pyrene concentration is increased from 10-5 to 10-2 M. Qualitatively, the overall attachment efficiency is higher in orthorhombic n-heneicosane than in liquid cyclohexane, but the selectivity of attachment is greater in cyclohexane. Differences between these results and those from irradiations of the same samples with eV range photons are discussed


Assuntos
Alcanos , Cicloexanos , Pirenos , Alcanos , Cicloexanos , Pirenos
12.
Rev. argent. microbiol ; 34(2): 72-76, abr.-jun. 2002.
Artigo em Inglês | LILACS | ID: lil-331802

RESUMO

Bacterial strains were isolated from contaminated waters, mud or soils. They are capable of growing in mineral medium with different chemicals as carbon source, such as aliphatic or aromatic hydrocarbons and polychlorinated biphenyls (PCBs). Most of these strains tolerate high concentrations (up to 30 v/v) of the xenobiotic substrates. This is particularly important for the development of fermenting processes to treat effluents or residues with a high content of contaminating compounds. An ion-specific potentiometric electrode (CO2) has been developed to measure CO2 production continuously. When the different strains were incubated in a mineral medium and in the presence of the corresponding substrate, a parallel between growth, substrate consumption and CO2 production was found. The developed system is suggested as an efficient and economical alternative to evaluate the potential of biodegradation by different microorganisms.


Assuntos
Alcaligenes , Carbono , Dióxido de Carbono/análise , Eletrodos , Hidrocarbonetos , Micrococcus , Potenciometria , Pseudomonas aeruginosa , Xenobióticos/metabolismo , Alcaligenes , Alcanos , Arocloros , Biodegradação Ambiental , Calibragem , Dióxido de Carbono/metabolismo , Poluentes Ambientais , Desenho de Equipamento , Fermentação , Micrococcus , Pseudomonas aeruginosa , Microbiologia do Solo , Estireno , Tolueno , Microbiologia da Água , Poluição Química da Água
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...