RESUMO
ABSTRACT PURPOSE: To test whether hemorrhagic shock (HS) increases the Cyclooxygenase-2 (COX-2) expression in the intestine and whether this enhanced COX-2 expression mediates the intestinal dysmotility after HS. METHODS: Male Wistar rats were randomly divided into HS sham group and HS group. At 180 min following HS establishment, the duodenum samples were harvested to assess the motility function, protein expression of COX-2 and the downstream products of COX-2, prostaglandins. RESULTS: Examination of motility function ex vivo showed that the contractile response to acetylcholine of smooth muscle strips of rats subjected to HS was significantly suppressed. A COX-2 inhibitor, NS-398, abolished this depressed contractile response after HS. Western blotting revealed an increased protein expression of COX-2 in intestinal tissues of HS rats. Immunohistochemical examination indicated that intestine tissues of HS rats were manifested by part of villous expansion and disruption, a large amount of COX-2 positive cells appearance in lamina propria and submucosa. Furthermore, the contents of prostaglandin E2 was significantly increased in intestinal tissues of HS rats. CONCLUSION: The enhanced COX-2/ prostaglandin E2 involves in the hemorrhagic shock induced intestinal dysmotility.
Assuntos
Animais , Masculino , /metabolismo , Duodeno/fisiopatologia , Motilidade Gastrointestinal/fisiologia , Choque Hemorrágico/enzimologia , Acetilcolina/farmacologia , Dinoprostona/metabolismo , Modelos Animais , Contração Muscular/efeitos dos fármacos , Nitrobenzenos/farmacologia , Distribuição Aleatória , Ratos Wistar , Choque Hemorrágico/fisiopatologia , Sulfonamidas/farmacologiaAssuntos
Aminas , Derivados de Benzeno , Dinitrobenzenos , Metemoglobinemia , Metilenobis (cloroanilina) , Nitrobenzenos , Tolueno , Trinitrotolueno , ToxicologiaRESUMO
Os autores relatam um surto de metemoglobinemia na Enfermaria de Pediatria e Bercario Externo do HCRP, causado por tinta de marcar fraldas contendo nitrobenzol (anilina). Foram acometidas 26 criancas, sendo mais afetadas as que pesavam menos de 6 kg. Apos remocao das fraldas, retirada das criancas das incubadoras, administracao de acido ascorbico e azul de metileno, houve boa evolucao clinica com recuperacao de todos os casos. Ressaltam os autores a importancia da profilaxia evitando-se o uso de tintas com anilina ou, entao, a fervura ou autoclavagem das roupas marcadas com tais tintas devendo ser entregues ao uso somente 12 horas apos aquele procedimento
