Análisis microbiológico a corto y largo plazo del material usado para esterilizar instrumental odontológico / Short and long-term microbiological analysis of the material for sterilize dental instruments

Introducción: el material para empaquetar el instrumental odontológico, como pueden ser bolsas de tela, papel o plástico, es usado por profesionales de la salud; sin embargo, es necesario esclarecer la efectividad de cada uno y determinar el tiempo que permanece estéril luego del procedimiento. Objetivo: identificar la eficacia de tela, plástico y papel como materiales para esterilizar instrumental a corto y largo plazo. Material y métodos: se realizaron cultivos sólidos y líquidos de instrumental esterilizado en tres materiales y con diferentes tiempos de postesterilización. Se incubaron a 36 oC por 72 horas en condiciones aerobias y anaerobias. Los resultados se analizaron usando una prueba de Kruskal-Wallis, seguida de una prueba de Dunn. Resultados: los resultados mostraron que inmediatamente después del proceso de esterilización, los tres materiales son efectivos (Kruskal-Wallis test, p = 0.2752), 24 horas (p = 0.2492), siete (p = 0.0509) y 14 días (p = 0.0006). Veinticuatro horas posterior a la esterilización la tela no es efectiva, el plástico disminuye su efectividad y el papel sigue siendo efectivo. Conclusión: en nuestros resultados, el papel es la mejor opción para esterilizar instrumental (AU)

Introduction: material such as cloth, paper or plastic bags to wrap dental instruments is used by health professionals, however, it is necessary to clarify the effectiveness of each one and determine if it remains sterile after the procedure. Objective: to determine the effectiveness of cloth, plastic and paper as materials to sterilize dental instruments in the short and long term. Material and methods: we carry out solid and liquid cultures of sterilized instruments in three materials, at different post-sterilization times, incubated at 36 oC for 72 hours under aerobic and anaerobic conditions, and the results were analyzed using a Kruskal-Wallis test, followed by from a Dunn's test. Results: our results showed that immediately after the sterilization process the three materials are effective (Kruskal-Wallis; p = 0.2752), 24 hours (p = 0.2492), 7 (p = 0.0509) and 14 (p = 0.0006) days. Twenty-four hours after the cloth is not effective, plastic decreases its effectiveness and paper remain effective. Conclusion: in our results, paper is the best option to sterilize dental instruments (AU)

Contaminación ambiental por plásticos durante la pandemia y sus efectos en la salud humana / Environmental pollution by plastics during the pandemic and its effectson human health

La historia del plástico se remonta a mediados del siglo XIX, y se considera que se origina por el interés de cambiar la materia prima en la fabricación de las bolas de billar, hechas originalmente en marfil. Desde entonces y a lo largo de muchos años, el polietileno, cloruro de polivinilo, poliestireno, polimetilmetacrilato, polietilentereftalato (PET), las poliamidas y otras sustancias similares han formado parte del día a día de la humanidad, a tal punto que algunos expertos en el tema consideran que estamos viviendo "La era del plástico". Todos los insumos y elementos plásticos han facilitado la vida, pero también han causado una gran contaminación ambiental que afecta la fauna, la flora y por supuesto al ser humano. La gran mayoría de los países han comprendido esta situación y han promulgado leyes o diseñado estrategias con el fin de contener el uso inadecuado y la generación de la contaminación causada por el plástico. Muchas de estas medidas han sido frenadas e incluso revertidas debido a la pandemia por COVID-19, que además de todas las afectaciones conocidas, ha causado un desmesurado incremento en el uso de materiales plásticos, como los elementos de protección personal, con el consecuente aumento de la contaminación y los riesgos que esta genera en la salud humana. Estos temas son tratados en este artículo, con el fin de concientizar al personal médico y a la población en general

The history of plastic dates back to the mid-nineteenth century, and it is considered that it originates from the interest in changing the raw material in the manufacture of billiard balls, originally made of ivory. Since then and for many years, polyethylene, polyvinyl chloride, polystyrene, polymethyl methacrylate, polyethylene terephthalate (PET), polyamides and other similar substances have been part of humanity's daily life, to the point that some consider that we are living 'The era of plastic'.All the inputs and plastic elements have made life easier, but they have also caused great environmental pollution that affects fauna and flora, and of course the human being. The vast majority of countries have understood this situation, have enacted laws and designed strategies in order to contain the inappropriate use and generation of pollution caused by plastic.Many of these measures have been stopped and even reversed due to the COVID-19 pandemic, which in addition to all the known effects, has caused a disproportionate increase in the use of plastic materials, such as personal protection elements with the consequent increase in pollution and the risks it generates for human health. These issues are addressed in this article, in order to raise awareness among medical personnel and the general population

Saneamiento industrial: Biodisponibilidad de patógenos en superficies / Industrial sanitation: Bioavailability of pathogens on surfaces

En condiciones adecuadas como humedad, alcalinidad, o temperatura, determinados patógenos logran adherirse a las superficies y sobrevivir ciertos períodos fuera de un anfitrión, persistiendo en algunos casos a procesos deficientes de limpieza y desinfección, configurándose como un posible foco de transmisión. Por ello, el correcto saneamiento cumple un propósito vital en la protección de los trabajadores de la industria y otros sectores frente al riesgo de contaminación por contacto directo con las superficies contaminadas. La literatura científica muestra amplia evidencia de la supervivencia de patógenos sobre superficies que son habituales dentro de instalaciones industriales, como acero, aluminio, madera, plástico y vidrio. La supervivencia de microorganismos en las superficies puede configurarse como candidato a marcador de biodisponibilidad, que puede ser usado en la industria para establecer y auditar los planes de higienización y saneamiento industrial, permitiendo estudiar la eficacia de los compuestos usados en la desinfección, y variables como su concentración, temperatura, e intervalos de aplicación y remoción(AU)

Under suitable conditions such as humidity, alkalinity, or temperature, certain pathogens manage to adhere to surfaces and survive certain periods outside of a host, persisting in some cases to poor cleaning and disinfection processes, becoming a possible source of transmission. Therefore, proper sanitation serves a vital purpose in protecting workers in industry and other sectors from the risk of contamination by direct contact with contaminated surfaces. The scientific literature shows ample evidence of the survival of pathogens on surfaces that are common within industrial facilities, such as steel, aluminum, wood, plastic and glass. The survival of microorganisms on surfaces can be configured as a candidate for bioavailability marker, which can be used in the industry to establish and audit industrial sanitation and sanitation plans, allowing to study the efficacy of the compounds used in disinfection, and variables such as its concentration, temperature, and application and removal intervals(AU(

Higienización y eliminación fúngica en instalaciones industriales para la disminución de riesgos ocupacionales / Sanitization and fungal elimination in industrial facilities to reduce occupational risks

Se ha comprobado que la exposición a bioaerosoles se asocia con varios efectos sobre la salud, como enfermedades pulmonares y alergias. El presente estudio transversal tuvo como objetivo investigar la contaminación por hongos en varias superficies pertenecientes a dos industrias de alimentos. La toma de muestra se realizó en tres semanas, inmediatamente después de realizada la limpieza y desinfección y antes de iniciar la producción. Se recolectaron 400 muestras de superficie (vidrio, acero inoxidable, aluminio, goma y plástico), también se evaluó la eficacia del programa de higienización en el control de la población de hongos. Todas las muestras presentaron recuentos fúngicos <10 ufc/cm2, sin haber diferencias significativas entre los tipos de superficie, sin embargo, las superficies plásticas exhibieron mayor crecimiento logarítmico de los hongos. Se determinó que 49,60% de las especies identificadas correspondieron al género Penicillium. Aunque los niveles de hongos en las industrias estudiadas fueron inferiores a los niveles recomendados por la Organización Mundial de la Salud, algunas medidas de salud ambiental como lavar y desinfectar las superficies después de cada turno de trabajo, y se recomiendan inspecciones periódicas para garantizar la seguridad de los trabajadores y de los productos que allí se manufacturan(AU)

It has been proven that exposure to bioaerosols is associated with several health effects, such as pulmonary diseases and allergies. The present crosssectional study aimed to investigate fungal contamination on various surfaces belonging to two food industries. The sampling was carried out in three weeks, immediately after cleaning and disinfection and before starting production. 400 surface samples were collected (glass, stainless steel, aluminum, rubber and plastic), the effectiveness of the sanitation program in controlling the fungal population was also evaluated. All the samples presented fungal counts <10 cfu / cm2, with no significant differences between the types of surfaces, however, the plastic surfaces exhibited higher logarithmic growth of the fungi. It was determined that 49.60% of the identified species corresponded to the Penicillium genus.Although the levels of fungi in the studied baths were lower than the levels recommended by the World Health Organization, some environmental health measure ssuch as washing and disinfecting surfaces after each working shift and periodic inspections are recommended ensuring the safety of the workers and the products that are manufactured there(AU)

Efficacy and safety of endoscopic ultrasound-guided drainage of pancreatic pseudocysts using double-pigtail plastic stents: A single tertiary center experience

OBJECTIVES: Pancreatic pseudocysts (PPC) are fluid collections with a well-defined wall that persist for more than 4 weeks inside or around the pancreas as a result of pancreatic inflammation and/or a ductal lesion. PPC have been successfully treated with endoscopic ultrasound (EUS)-guided drainage using different stents. This study aimed to evaluate the safety and efficacy of EUS-guided drainage of PPC using double-pigtail plastic stents in a tertiary hospital. METHODS: Patients with PPC referred for EUS-guided drainage between May 2015 and December 2019 were included in this case series. The primary endpoint was to evaluate the efficacy (clinical success) and safety (adverse events and mortality) of EUS-guided drainage of PPC. Secondary endpoints included technical success and pseudocyst recurrence. RESULTS: Eleven patients (mean age, 44.5±18.98 years) were included in this study. The etiologies for PPC were acute biliary pancreatitis, chronic alcoholic pancreatitis, and blunt abdominal trauma. The mean pseudocyst size was 9.4±2.69 cm. The clinical success rate was 91% (10/11). Adverse events occurred in three of 11 patients (27%). There were no cases of mortality. The technical success rate was 100%. Pseudocyst recurrence was identified in one of 11 patients (9%) at 12 weeks after successful clinical drainage and complete pseudocyst resolution. CONCLUSION: EUS-guided transmural drainage of PPC using double-pigtail plastic stents is safe and effective with high technical and clinical success rates.

BPA toxicity during development of zebrafish embryo / Toxicidade do BPA durante o desenvolvimento de embriões de Zebrafish

Bisphenol A (BPA) is a monomer used in the production of polycarbonate, a polymer commonly found in plastics, epoxy resins and thermal papers. The presence of BPA in food, water, air and dust has been of great concern in recent years not only due to environmental and ecological issues but also because of its supposed risk to public health related to its mutagenic and carcinogenic potential. In this study we evaluated the toxicity of bisphenol A in zebrafish embryos (Danio rerio) and determined the 50% lethal concentration (LC50) of this chemical. BPA was used at concentrations ranging from 1 µM to 100 µM in E3 medium/0.5% dimethylsulfoxide (DMSO) from previously prepared stock solutions in 100% DMSO. Controls included embryos exposed only to E3 medium or supplemented with 0.5% DMSO. Camptothecin (CPT), a known inhibitor of cell proliferation was used as positive control at a concentration of 0.001 µM in E3 medium/0.5% DMSO. Adults zebrafish were placed for breeding a day before the experimental set up, then, viable embryos were collected and selected for use. Experiments were carried out in triplicates, according to specifications from Organization for Economic Cooperation and Development (OECD). One embryo/well (25 embryos per concentration) was distributed in 96 well microplates in presence or absence of the chemicals. The plates were kept in BOD incubators with a controlled temperature of 28.5 ºC and with photoperiod of 14 h light:10 h dark. After 24h, 48h, 72h and 96h exposure, the exposed embryos were evaluated according to the following parameters: mortality, coagulation, rate of heartbeat, hatching and presence of morphological abnormalities. Photography was obtained by photomicroscopy. Apoptosis was evaluated by DNA ladder assay. DNA was extracted by phenol:chloroform method and analyzed by 2% agarose gel electrophoresis. DNA fragments were visualized after ethidium bromide staining in ultraviolet transilluminator. The LC50 determined for BPA was 70 µM after 24 hours, 72 µM after 48 hours, 47 µM after 72 hours and 31 µM after 96 hours exposure. BPA induced morphological and physiological alterations such as yolk sac and pericardial edema, hatching delay or inhibition, spine deformation, decreasing in heartbeat rate and mortality. In conclusion, this study demonstrated that BPA induced marked malformations in zebrafish embryos at concentrations above 25 µM corroborating the current concerns related to the widespread presence of BPA in the air, food and water used by humans as well as in the bodily fluids and tissues.

Bisfenol A (BPA) é um monômero utilizado na produção de policarbonato, um polímero comumente encontrado em plásticos, resinas epóxi e papéis térmicos. A presença de BPA em alimentos, água, ar e poeira tem sido motivo de grande preocupação nos últimos anos, não só devido a questões ambientais e ecológicas, mas também ao suposto risco para a saúde pública relacionado ao seu potencial mutagênico e carcinogênico. Neste estudo avaliamos a toxicidade do bisfenol A em embriões de peixe-zebra (Danio rerio) e determinamos a concentração letal 50% (LC50) deste composto químico. O BPA foi usado na faixa de concentração entre 1 µM e 100µM em meio E3/0,5% de dimetilsulfóxido (DMSO), preparado a partir de soluções estoques em 100% DMSO. Os controles negativos incluíram embriões expostos apenas ao meio E3 ou suplementado com 0,5% DMSO. Camptotecina (CPT), um conhecido inibidor da proliferação celular, foi usado como controle positivo a uma concentração de 0,001 µM em meio E3/0,5% DMSO. Peixes-zebra adultos foram colocados para reprodução um dia antes da montagem experimental, em seguida, embriões viáveis foram coletados e selecionados para uso. Os experimentos foram realizados em triplicata, de acordo com as especificações da Organização para Cooperação e Desenvolvimento Econômico (OCDE). Um embrião/ poço (25 embriões por concentração) foi distribuído em microplacas de 96 poços na presença ou ausência dos compostos químicos. As placas foram mantidas em incubadoras BOD com temperatura controlada de 28,5 ºC e com fotoperíodo de 14h claro:10h escuro. Após 24h, 48h, 72h e 96h, os embriões expostos foram avaliados de acordo com os seguintes parâmetros: mortalidade, presença de coagulação, taxa do batimento cardíaco, eclosão e presença de anormalidades morfológicas. Fotografias foram obtidas por fotomicroscopia. A apoptose foi avaliada pelo ensaio de DNA ladder. O DNA foi extraído pelo método fenol:clorofórmio e analisado por eletroforese em gel de agarose a 2%. Fragmentos de DNA foram visualizadas após coloração com brometo de etídio em um transiluminador ultravioleta. A LC50 determinada para o BPA foi 70 µM após 24 horas, 72 µM após 48 horas, 47 µM após 72 horas e 31 µM após exposição por 96 horas. O BPA induziu alterações morfológicas e fisiológicas como edema de saco vitelino e edema pericárdico, atraso no tempo ou inibição da eclosão, deformação da coluna vertebral, diminuição da taxa de batimentos cardíacos e mortalidade. Em conclusão, este estudo demonstrou que o BPA induziu grande número de malformações em embriões de peixe-zebra em concentrações acima de 25 µM, corroborando as preocupações atuais relacionadas a presença generalizada do BPA no ar, alimento e água usados pelos seres humanos bem como nos fluidos e tecidos corporais.

Levels of BPA in makdous, a traditional Syrian food, using solid-phase extraction followed by HPLC

Bisphenol A (BPA), an endocrine disrupter, can migrate from packaging material into food stuff. This research was designed to measure BPA levels in makdous, a traditional Syrian food. Forty three samples of makdous stored in different plastic containers (polyethylene (PE), polypropylene (PP), and unspecified plastic containers) were analyzed every 3 months for one year beginning July 2017. Quantification of BPA was carried out by an RP-HPLC system equipped with fluorescence detector after solid phase extraction. Migration was found in PE and PP plastic containers with slight differences. Statistically significant differences in BPA levels were observed between samples assayed after two weeks of preparation and samples assayed after 12 months (mean 16.32 vs. 38.26 µg/kg, p value=0.003). According to these amounts, BPA levels were all under the specific migration limit of 0.05 mg/kg as newly referred in Regulation (EU) No 2018/213. These levels of exposure would only contribute to 2.15% and 2.75% of the EFSA t-TDI in both men and women respectively based on mean dietary exposure estimates derived from a 24-h dietary information study from 875 participants. Hence there are no concerns about potential health risks from makdous consumption

Suicidio por confinamiento con bolsa plástica y gas helio: presentación de dos casos / Suicide with plastic bag and helium gas: two case report

Resumen El confinamiento al igual que el sepultamiento son parte de las sofocaciones por carencia de aire respirable. Su etiología principal es la accidental, como ocurre con los mineros o los niños que juegan con bolsas de plástico o quedan atrapados dentro de lugares sin ventilación. En la actualidad se ha visto el aumento a nivel mundial sobre todo en países desarrollados, del uso de una bolsa plástica en conjunto con algún gas noble (principalmente helio) como método suicida "per se" o como proceso final de eutanasia. En el presente artículo se hará revisión de dos casos en Costa Rica, con sus hallazgos en la necropsia, la carencia de hallazgos toxicológicos y la importancia del escenario de muerte para poder emitir un criterio desde el punto de vista médico legal.

Abstract The confinement as well as the burial are part of the suffocations due to lack of breathable air. Its main etiology is accidental, as it happens with miners or the children who play with plastic bags or get trapped inside unventilated places. At present, the increase in the world has been seen especially in developed countries of the use of a plastic bag in conjunction with some noble gas (mainly helium) as a suicidal method or as a final process of euthanasia.

Diseño innovador para la obtención y caracterización de un bioplástico utilizando como materia base la fibra de la cáscara de coco y papaya / Innovative design for obtaining and characterizing a bioplastic using as a base material the fiber of the coconut shell and papaya fiber

Los plásticos se producen a partir del petróleo. Estos polímeros perduran en la naturaleza por largos períodos de tiempo y, por tanto, se acumulan, generando así grandes cantidades de residuos sólidos. El objetivo principal de esta investigación es producir, a escala de laboratorio, un bioplástico a partir de la fibra contenida en la cáscara de coco, que pueda servir de materia prima para la elaboración de productos biodegradables.

Plastics are produced from oil. These polymers persist in nature for long periods of time and, therefore, they are accumulated in large amounts of solid waste. The main objective of this research is to produce, on a laboratory scale, a bioplastic from the fiber contained in the coconut shell, which can serve as raw material for the production of biodegradable products.

Papel de distintas técnicas de imagen para identificar y localizar cuerpos extraños intraoculares relacionados con trauma ocular abierto: reporte de tres casos y revisión de la literatura / The role of various imaging techniques in identifying and locating intraocular foreign bodies related to open-globe injury: three case reports and literature review

ANTECEDENTES La ubicación precisa de un cuerpo extraño intraocular es crucial para el manejo de pacientes con trauma ocular abierto. La tomografía computarizada se usa habitualmente para detectar su ubicación en el segmento posterior. Reportamos tres casos con diferentes cuerpos extraños intraoculares en el segmento posterior, que fueron localizados con precisión mediante la tomografía computarizada y ecografía modo B. PRESENTACIÖN DEL CASO Presentamos tres casos con diferentes mecanismos de trauma, tipos de cuerpo extraño intraocular, síntomas clínicos y pronóstico visual. La tomografía computarizada determinó la ubicación exacta de todos los cuerpos extraños intraoculares en el segmento posterior. El ultrasonido modo B se realizó en un paciente con un cuerpo extraño intraocular no metálico. El primer caso tuvo una lesión ocular perforante con un cuerpo extraño intraorbitario; el caso dos y el caso tres presentaron diferentes tipos de cuerpos extraños intraoculares con pronóstico diferente. El manejo y el pronóstico fue distinto en todos los casos; todos se manejaron con éxito. La ubicación exacta de los cuerpos extraños intraoculares utilizando las diferentes modalidades de diagnóstico es importante en estos pacientes. Estos casos sirven como recordatorio de que el uso adecuado de las pruebas de imagen es indispensable en el contexto de un cuerpo extraño intraocular relacionado con trauma ocular abierto. CONCLUSIÓN Las diferentes técnicas de imágenes son muy importantes para la detección de un cuerpo extraño intraocular. La tomografía computarizada es una de las modalidades de imagen más simple y efectiva para la localización de cuerpos extraños intraoculares relacionadas con trauma ocular abierto.

BACKGROUND: Determining the precise location of intraocular foreign bodies is crucial for the management of patients with open-globe injury. Computed tomography is the most common method for detecting intraocular foreign bodies in the posterior segment. In this article, we describe three cases of open-globe injury with different types of intraocular foreign bodies in the posterior segment that were accurately located using computed tomography scans and B-scan ultrasonography. CASE PRESENTATION: Each of the three cases of open-globe injury described in this report had different types of ocular trauma, clinical symptoms, and intraocular foreign bodies. Computed tomography scans showed the exact location of the intraocular foreign bodies in the posterior segment in two of the three cases. A B-scan ultrasound was used to determine the location of a non-metallic intraocular foreign body in the third case. All three patients had intraocular foreign bodies, and one of them had an additional orbital foreign body. Case 1 had a perforating eye injury with the additional intraorbital foreign body; Cases 2 and Case 3 had different types of intraocular foreign bodies and prognoses. Various treatment approaches were used, ranging from observation to surgery, depending on the location of the intraocular foreign bodies, and all cases were successfully managed. These three cases show that proper use of various types of imaging tests is indispensable in the context of an intraocular foreign body related to open-globe injury. CONCLUSION: Imaging techniques are crucial for the detection of an intraocular foreign body, and computed tomography is one of the simplest and most useful, especially in cases of open-globe injury.

Effectiveness of Two Types of Photostimulable Phosphor Plate Plastic Barrier Envelopes for Prevention of Microbiological Contamination

Abstract Objective: To compare the effectiveness of two types of commercially available photostimulable phosphor plate (PSP) protective barrier envelopes to prevent microbiological contamination. Material and Methods: In this cross-sectional study, 80 barrier envelopes were tested in 40 volunteers. The PSP plates were placed individually in Asia Teb and Soredex protective barrier envelopes and were placed in the mouth for two minutes, similar to periapical films. The protective barrier envelopes were then removed under sterile conditions, and the sensors were placed on different culture media. The number of colonies on each plate was counted. Data were analyzed using SPSS via McNemar and Wilcoxon tests. Results: Bacterial growth was noted in 17.5% of PSPs with Soredex, and 32.5% of PSPs with Asia Teb barrier envelopes. Gram-positive bacilli were the most commonly isolated bacteria. The difference between the Asia Teb and Soredex barrier envelopes for the protection of microbiological contamination was not significant (p>0.05). Conclusion: The use of different types of protective barrier envelopes was not sufficient for prevention of microbiological contamination of PSP plates, and some adjunct modalities were required to decrease microbiological contamination of PSP plates.

Identificação de perigos microbiológicos associados às tábuas de corte de polietileno de uma Unidade de Alimentação do município de Fortaleza-CE / Identification of microbiological hazards associated with the polyethylene cutting tables of a power supply unit of the municipality of Fortaleza-CE

As Unidades de Alimentação e Nutrição são locais com o intuito de fornecer alimentos de maneira adequada e segura para os comensais. Para isso faz-se necessário que os procedimentos e utensílios, tais como as placas de corte, usados na produção de alimentos sejam seguros. Assim o objetivo do trabalho foi avaliar e identificar os perigos microbiológicos associados às tábuas de corte de polietileno utilizadas por uma Unidade de Alimentação e Nutrição de Fortaleza-CE. Por meio da técnica de Swab, foram coletadas 5 amostras de tábuas de corte para análise microbiológica de coliformes termotolerantes e contagem de aeróbios mesófilos. Constatou-se que 60% (3 unidades) das tábuas analisadas encontravam-se em condições satisfatórias e em 40% (2 unidades) condições insatisfatórias, o que sugere que essas placas de corte podem ser consideradas um risco à saúde dos comensais.

Determinação das características físico-químicas e das miopatias do músculo Pectoralis major em frangos de corte criados em piso de plástico perfurado / Determination of the physical-chemical characteristics and myopathy of the Pectoralis major muscle in broiler chickens raised on perforated plastic floor

Esse estudo teve como objetivo avaliar a influência da criação de frangos de corte em pisos plásticos perfurados sobre a qualidade físico-química da carne e a incidência de miopatias peitorais. Para isso, os animais foram alojados em 14 box, em dois tratamentos, cama e piso, 25 animais por box. Para a capacidade de retenção de água as aves do tratamento piso apresentaram melhores resultados; no entanto, apresentaram maior perda de água por cocção. O músculo Pectoralis major dos animais criados no piso de plástico, apresentou menor valor de força de cisalhamento, entretanto, maior força de deformação. Para as miopatias, o peito dos frangos criados no piso de plástico apresentaram maior incidência de white striping, enquanto os que permaneceram na cama de casca de arroz apresentaram maior incidência de wooden breast. A criação de frangos de corte em pisos de plástico perfurados não influenciou as características físico-químicas da carne, bem como a incidência de miopatias peitorais.

Psicologia ambiental e recursos em sustentabilidade: revisão integrativa / Environmental psychology and sustainability resources: integrative review / Psicología ambiental y recursos de sostenibilidad: revisión integradora

O objetivo deste estudo foi examinar, por meio de revisão integrativa de artigos empíricos, a produção científica relacionando a Psicologia Ambiental (variáveis de comportamento/cognição) e sustentabilidade, considerando diferentes recursos. Para efeitos deste estudo, foram analisados os artigos que abordam um recurso único. A busca foi realizada nos periódicos Environment and Behavior, Journal of Environmental Psychology e Psyecology, reconhecidos pela relevância internacional em produção científica no campo da Psicologia Ambiental. Foram selecionados 24 artigos, publicados entre os anos de 2012 e 2016, a partir dos critérios de inclusão pré-estabelecidos. Os anos com maior número de publicação foram 2014 (n = 8) e 2015 (n = 8). A maioria dos artigos se refere a estudos realizados na Europa, apenas um trata de pesquisa no Brasil. Os recursos abordados nos estudos abrangeram transporte, produtos, água, energia elétrica, sacola plástica e pastagem natural. Os resultados revisados demonstram que há uma diversidade de conceitos na mediação entre comportamentos/cognições e diferentes recursos, indicando a relevância destas abordagens para a promoção de sustentabilidade. Sugere-se caminhos para pesquisa e intervenção nas relações sustentáveis entre pessoas e recursos disponíveis em seus meios.

The objective of this study was to examine, through an integrative review of empirical articles, the scientific production on the relation between Environmental Psychology (behavior/cognition variables) and sustainability considering different resources. For that purpose, this analysis considered articles approaching a unique resource. Search was conducted in three journals with international relevance in the scientific production of Environmental Psychology: Environment and Behavior, Journal of Environmental Psychology and Psyecology. Following pre-established inclusion criteria, results included 24 articles published from 2012 and 2016. Years with higher number of publications were 2014 (n = 8) and 2015 (n = 8). The articles found refer mostly to studies conducted in Europe; only one article is a research from Brazil. Resources included transportation, products, water, energy, plastic bag, and natural grassland. Results indicate that there is a diversity of concepts mediating behaviors/cognitions and different resources, which reveals the relevance of these approaches to the promotion of sustainability. Research and intervention paths are suggested to sustainable relations between people and resources available in their surroundings.

El objetivo de este estudio fue examinar, por medio de una revisión integradora de artículos empíricos, la producción científica que relaciona la Psicología Ambiental (variables de comportamiento/cognición) y la sostenibilidad, considerando diferentes recursos. Para los propósitos de este estudio, se analizaron artículos que abordan un solo recurso. La búsqueda fue realizada en los periódicos Environment and Behavior, Journal of Environmental Psychology y Psyecology, reconocidos por la relevancia internacional en producción científica en el campo de la Psicología Ambiental. Se seleccionaron 24 artículos, publicados entre los años 2012 y 2016, basados en criterios de inclusión preestablecidos. Los años con el mayor número de publicaciones fueron 2014 (n = 8) y 2015 (n = 8). La mayoría de los artículos se refiere a estudios realizados en Europa, sólo uno trata de investigación en Brasil. Los recursos abordados en los estudios incluyeron transporte, productos, agua, energía eléctrica, bolsas de plástico y pastoreo natural. Los resultados revisados demuestran que hay una diversidad de conceptos en la mediación entre comportamientos/cogniciones y diferentes recursos, indicando la relevancia de estos enfoques para la promoción de la sostenibilidad. Se sugieren caminos para la investigación e intervención en las relaciones sostenibles entre personas y recursos disponibles en sus medios.

Implementación de un programa de reciclaje en pabellón / Performance of a recycling program in the operating room

INTRODUCTION: The waste management of a hospital center affects people both those who receive health care and the environment that receives them. The impact on the environment of the task of peri-operative care has not yet been properly studied. It is known that of the 105 tons of garbage monthly from the health center understudy go to the landfills of the municipality. However, there are different elements, such as low-density plastics (LDP), which can be recycled. OBJECTIVE: implement a clean low-density plastics recycling program in the central Operating Room of the UC-Christus Clinical Hospital. METHODOLOGY: In this report, a prospective longitudinal study was conducted with a sample for convenience, in which an intervention was scheduled in series repeated over time from 2017 to 2018. Residents of anesthesia, anesthesiologists, nurses, nurse technicians, and hospital support personnel participated. RESULTS: In total 200 employees participated. During the pilot phase, 30 kg of plastics were quantified, later increasing to 100 kg of LDP in the subsequent phases. It began with the transfer phase to other pavilions within the healthcare center and to other centers in the metropolitan region. Finally, an alternative was started with the municipalities. CONCLUSIONS: It was possible to quantify the amount of clean LPD for recycling. It was possible to involve the collaborators of the institution. This is the first program reported that involves an intervention for LPD.

INTRODUCCIÓN: El manejo de los desechos de un centro hospitalario afecta a las personas tanto a los que reciben atención de salud como también al medioambiente que recibe éstos. El impacto en el ambiente del quehacer de la atención perioperatoria aún no ha sido estudiado apropiadamente. Se sabe que 105 toneladas de basura mensuales del centro asistencial en estudio van a los vertederos de la municipalidad. Sin embargo, existen distintos elementos, como los plásticos de baja densidad (PBD), limpios los cuales pueden ser reciclados. OBJETIVO: Implementar de un programa de reciclaje de plásticos de baja de densidad limpios en el Servicio de Pabellón Central del Hospital Clínico UC-Christus. MATERIAL Y MÉTODOS: En este reporte se realizó un estudio prospectivo longitudinal con una muestra por conveniencia, en la cual se programó una intervención en series repetidas en el tiempo durante 2017 al 2018. Participaron residentes de anestesia, anestesiólogos, enfermeras, TENS, personal de apoyo del hospital entre otros. RESULTADOS: En total 200 colaboradores participaron. Durante la fase piloto se cuantificó 30 kg de plásticos, posteriormente, se incrementó a 100 kg de PBD en las fases posteriores. Se comenzó con la fase de transferencia hacia otros pabellones dentro del centro asistencial y hacia otros centros de la Región Metropolitana. Finalmente, se comenzó a buscar alternativa con las municipalidades. CONCLUSIONES: Se logró cuantificar la cantidad de PBD limpios para reciclar. Se logró involucrar a los colaboradores de la institución. Este es el primer programa reportado que involucra una intervención para el PBD.

Seguridad y eficacia del drenaje guiado por endosonografía de colecciones pancreáticas usando prótesis plásticas y metálicas tipo LAMS / Safety and efficacy of EUS-guided drainage of pancreatic fluid collections using plastic and lumen apposing metal stents (LAMS)

Background: Pancreatic fluid collections (PFC) are associated with severe acute pancreatitis. After maturation (after 4 weeks) they are classified as pancreatic pseudocyst (PP) and walled-off necrosis (WON). Endoscopic management is associated with lower morbidity and mortality compared with surgery. Aim: To describe the experience of EUS-guided drainage PFC drainage using plastic stent (PS) or metal stent (lumen apposing metal stents, or "LAMS"). Methods: Retrospective, observational study, between 2016-2019. Results: 17 patients in total. 8 cases were symptomatic PP and 9 were WON. Age 12-72 years. 12 cases in men. Indications: abdominal pain 5 cases and infection 11. The 8 cases of PFC were drained successfully using PS, without adverse effects. Regarding WON drainage, 3 cases were managed with PS and 6 with LAMS. In 5 cases, complementary endoscopic necrosectomy was required. A patient with LAMS required complementary surgical cleaning ("step-up") to treat collections far from the LAMS. Complications: one case of PS presented pigment occlusion with superinfection and another case presented migration to the stomach. Regarding LAMS, one case presented self-limited bleeding, another case presented obstruction of the stent with infection, which was managed endoscopically and with antibiotics. Conclusion: The endoscopic management of PFC is effective and safe, with plastic and metal stent (LAMS). The choice of type of stent depends on the characteristics of PFC (liquid vs solid), center experience and costs.

Introducción: Las colecciones pancreáticas (CP) se asocian a pancreatitis agudas graves. Luego de su maduración (después de las 4 semanas) se clasifican en pseudoquistes (PQ) y necrosis encapsulada (NE). El manejo endoscópico se asocia a menor morbimortalidad comparado con el quirúrgico. Objetivo: Describir la experiencia del drenaje de CP mediante el uso de prótesis plásticas (PP) o metálicas (lumen apposing metal stents, o "LAMS") mediante endosonografía. Método: Estudio retrospectivo, observacional, entre 2016-2018. Resultados: 17 pacientes en total. Ocho casos fueron PQ sintomáticos y 9 NE. Edad 12-72 años (12 casos en hombres). Indicaciones: dolor abdominal 5 casos e infección 11. Los 8 casos de PQ fueron drenados exitosamente con PP, sin efectos adversos. En relación con el drenaje de NE, 3 casos fueron manejados con PP y 6 con LAMS. En 5 casos se requirió necrosectomía endoscópica complementaria. Un paciente con LAMS requirió aseo quirúrgico complementario por colecciones alejadas al LAMS. En relación con las complicaciones, un caso de PP presentó oclusión del pigtail con sobreinfección y otro presentó migración al estómago. Respecto a LAMS, un caso presentó sangrado autolimitado y otro caso obstrucción de la prótesis con sobreinfección, que fue manejada endoscópicamente y con antibióticos. Conclusión: El manejo endoscópico de las CP es efectiva y segura, tanto con prótesis plásticas como metálicas (LAMS). La elección de la prótesis depende de las características de la lesión (líquido vs sólido), experiencia del centro y costos.

Contaminação microbiana em canudos plásticos coletados em lanchonetes e bares de municípios do Vale do Taquari - RS / Microbial contamination in plastic straws collected in snack bars and bars of Taquari Valley municipalities - RS

Canudos plásticos utilizados para consumo de refrigerantes e outras bebidas podem influenciar na contaminação microbiana, devido à má fiscalização higiênico-sanitária na sua fabricação. Este estudo teve como objetivo quantificar os microrganismos presentes em canudos plásticos utilizados em bares e lanchonetes de municípios do Vale do Taquari, Rio Grande do Sul, Brasil. Trata-se de estudo quantitativo e descritivo. A análise das cinco amostras de canudos plásticos coletadas em cada um dos 15 bares e lanchonetes escolhidos aleatoriamente foi efetuada no laboratório de microbiologia da Universidade do Vale do Taquari, para os seguintes microrganismos: Staphylococcus aureus, Escherichia coli, Enterococcus spp., Pseudomonas aeruginosa e fungos. Considerou-se como amostra os cinco canudos coletados em cada bar ou lanchonete. Cada amostra foi analisada em triplicata. O resultado das análises não apontou a presença de Enterococcus spp. e Pseudomonas aeruginosa, mas identificou a presença de Staphylococcus spp. em cinco amostras (33,3%), de fungos em cinco amostras (33,3%) e de coliformes totais em uma amostra (6,6%). Concluiu-se que Staphylococcus spp., coliformes totais, bolores e leveduras (fungos) foram os microrganismos que prevaleceram nas amostras dos canudos plásticos utilizados em bares e lanchonetes de municípios do Vale do Taquari, Rio Grande do Sul, Brasil

Plastic straws used to consume soft drinks and other beverages may influence microbial contamination due to poor sanitary inspection in their manufacture. This study aimed to quantify the microorganisms present in plastic straws used in bars and snack bars of Taquari Valley municipalities, Rio Grande do Sul, Brazil. The analysis of the five samples of plastic straws collected at each of the 15 randomly chosen bars and snack bars was carried out in the microbiology laboratory of Univates, for the following microorganisms: Staphylococcus aureus, Escherichia coli, Enterococcus spp., Pseudomonas aeruginosa and fungi. The five straws collected in each bar or snack bar were considered as samples. Each sample was analyzed in triplicate. The results of the analyses did not indicate the presence of Enterococcus spp. and Pseudomonas aeruginosa, but identified the presence of Staphylococcus spp. in five samples (33.3%), fungi in five samples (33.3%) and total coliforms in one sample (6.6%). In conclusion, Staphylococcus spp., total coliforms, molds and yeasts (fungi) were the pearlescent microorganisms in the samples of plastic straws used in bars and snack bars of the municipalities of Taquari Valley, Rio Grande do Sul, Brazil

Minimally invasive endoscopic treatment of necrotizing pancreatitis: A case report with images and review of the literature / Tratamento endoscópico minimamente invasivo: relato de caso e revisão da literatura

Summary Necrotizing pancreatitis with fluid collections can occur as a complication of acute pancreatitis. The management of these patients depends on the severity and involves multiple medical treatment modalities, as clinical intensive care and surgical intervention. In this article, we show a severe case of walled-off pancreatic necrosis that was conducted by endoscopic drainage with great clinical outcome.

Resumo Pancreatite necrosante com coleções pode ocorrer como complicação da pancreatite aguda. O manejo desses pacientes depende da gravidade e envolve múltiplas modalidades médicas de tratamento, como terapia clínica intensiva e intervenção cirúrgica. Neste artigo, mostramos um caso grave de necrose pancreática com ótima resolução clínica após drenagem endoscópica.

Using plastic tips in artificial feeding of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) females / Uso de ponteiras plásticas na alimentação artificial de fêmeas de Rhipicephalus sanguineus

Abstract This study evaluated the influence of the initial weight, feeding period and temperature on weight gain and biological parameters of the non-parasitic phase of partially engorged Rhipicephalus sanguineus females that were artificially fed using plastic tips as feeding devices. The device did not alter the oviposition of the females or any other parameters evaluated. Furthermore, it was observed that the temperature of the feeding the group did not affect the weight gain and biology of ticks. This device has great potential for the development of studies on bioagent transmission because it provides higher intake of blood by ixodid ticks.

Resumo Este estudo avaliou a influência do peso inicial, período de alimentação e temperatura no ganho de peso e parâmetros biológicos da fase não parasitária, de fêmeas parcialmente ingurgitadas de Rhipicephalus sanguineus alimentadas artificialmente utilizando ponteiras plásticas como dispositivo de alimentação. O dispositivo não alterou a oviposição das fêmeas ou quaisquer outros parâmetros avaliados. Além disso, observou-se que a temperatura de alimentação do grupo não afetou o ganho de peso e a biologia dos carrapatos. Este dispositivo tem um grande potencial para o desenvolvimento de estudos sobre a transmissão de bioagentes, uma vez que proporciona maior ingestão de sangue por carrapatos ixodídeos.

Translucency and color match with a shade guide of esthetic brackets with the aid of a spectroradiometer

ABSTRACT Objective: Since the color of esthetic brackets should match that of teeth, the aims of this study were to determine the color and translucency of esthetic brackets by means of the clinically relevant use of a spectroradiometer, and to compare the color of brackets with that of a commercial shade guide. Methods: The color of central and tie-wing regions of four plastic and four ceramic brackets was measured according to the CIE L*a*b* color scale over white and black backgrounds. Brackets were classified into five groups based on their composition. The color of Vitapan Classical Shade Guide tabs was also measured. Translucency parameter (TP) and contrast ratio (CR) were calculated to determine translucency. Results: Color differences between brackets and the shade guide tabs were 10.4 - 34.5 ∆E*ab units. TP and CR values for the central region were 16.4 - 27.7 and 0.38 - 0.58, whereas for the tie-wings they were 24.0 - 39.9 and 0.25 - 0.45, respectively. The color coordinates, TP and CR values were significantly influenced by bracket composition and brand (p < 0.05). Conclusions: Esthetic brackets investigated herein showed unacceptable color differences (∆E*ab > 5.5) compared with the shade guide tabs. Differences in the translucency of brackets by brand were within the visually perceptible range (∆CR > 0.07). Therefore, brackets showing the best matching performance for each case should be selected considering esthetic and functional demands.

RESUMO Objetivo: considerando-se que a cor dos braquetes estéticos deve corresponder à cor dos dentes, os objetivos do presente estudo foram determinar a cor e a translucidez de braquetes estéticos, por meio do uso clinicamente relevante de um espectroradiômetro, e comparar a cor desses braquetes com a cor de uma escala comercial de cores. Métodos: a cor das regiões central e da aleta de quatro braquetes plásticos e quatro braquetes cerâmicos foi medida segundo o sistema de cores CIE L*a*b*, sobre fundos branco e preto. Os braquetes foram classificados em cinco grupos, de acordo com a sua composição. A cor das peças da escala de cores Vitapan Classical(r) também foi medida. O parâmetro de translucidez (PT) e a razão de contraste (RC) foram calculados para se determinar a translucidez. Resultados: as diferenças de cor entre os braquetes e as peças da escala de cores variaram de 10,4 a 34,5 unidades ∆E*ab. Os valores de PT e RC, respectivamente, variaram de 16,4 a 27,7 e de 0,38 a 0,58 na região central; já para a região das aletas, esses valores variaram de 24,0 a 39,9 e de 0,25 a 0,45. As coordenadas de cor e os valores de PT e RC foram significativamente influenciados pela marca e composição dos braquetes (p < 0,05). Conclusões: os braquetes estéticos investigados nesse estudo demonstraram diferenças de cor inaceitáveis (∆E*ab > 5,5), quando comparados às peças da escala de cores. As diferenças de translucidez entre as marcas de braquetes estavam dentro do limite visualmente perceptível (∆RC > 0,07). Portanto, ao selecionar os braquetes com a melhor correspondência de cor, deve-se levar em consideração as exigências estéticas e funcionais de cada caso.