Screening and Characterization of Bacillus Strains Producing Highly Thermostable Amylase from Various Hot Springs of Algeria

HIGHLIGHTS Screening extremophile Bacillus strains from various Hot Springs Characterization Of Bacillus Strains Producing Highly Thermostable Amylase Genetic identification of the best strains

Abstract Currently thermostable Amylase represents a broad biotechnological interest and desired by a various industries. In this study, forty-six bacterial strains belonging to the genus Bacillus were isolated from various hot springs in the North West of Algeria based on their ability to degrade starch and produce amylase in Starch Agar medium. The majority of isolates showed a positive amylolytic activity. In order to select the most thermostables amylase the effect of temperature on enzymes was estimated, therefore the study of amylase thermostability was culminated by the selection of Four Strains having an interesting optimum of activity and range of stability, reaching 75 °C for the strains HBH1-2, HBH1-3, HBH3-1and 85 °C for the strain HC-2, This indicates that the Enzyme produced by retained strains have optimum activity at high temperature. The identity of the selected strains was established on the basis of the morphological, biochemical characteristics and phylogenetic position as determined by 16S Ribosomal DNA gene sequencing. The whole strains belonged to the genus Bacillus and their phylogeny were also reported in this study.

Caracterización clinicoepidemiológica de niños argelinos con enfermedades infecciosas / Clinical epidemiological characterization of Algerian children with infectious diseases

Introducción: Los niños representan el futuro, y su crecimiento y desarrollo saludable deben ser una de las máximas prioridades para todas las sociedades, ellos son vulnerables frente a la malnutrición y enfermedades infecciosas, que son prevenibles o tratables en su gran mayoría, por lo que la Organización Mundial de la Salud crea estrategias para minimizar las causas que conspiren en contra de la salud de los niños y adolescentes. Métodos: Se realizó un estudio descriptivo y longitudinal de los 4 109 niños mayores de 28 días de nacidos y menores de 14 años de edad con enfermedades infecciosas, atendidos en el Servicio de Pediatría del Hospital Clinicoquirúrgico Docente Mohamed Boudiaf, de la provincia del Bayadh, en la República Popular y Democrática de Argelia, en el período 2017-2018. Objetivo: Caracterizar desde el punto de vista clinicoepidemiológico a los niños argelinos entre 28 días de nacidos y menores de 14 años de edad con enfermedades infecciosas. Resultados: Se obtuvo que 88,4 % de los integrantes del estudio padecieron de enfermedades infecciosas y 11,6 % no infecciosas; igualmente, los menores de un año y del sexo masculino fueron los que más enfermaron (58,0 %), en tanto, las enfermedades diarreicas agudas y las infecciones respiratorias resultaron ser las más frecuentes (48,0 y 32,0 %, respectivamente). Del total de pacientes, 3 607 evolucionaron favorablemente (99,8 %). Conclusiones: Las enfermedades infecciosas constituyen un problema de salud en Argelia que sigue aportando niños enfermos y fallecidos por esta causa; asimismo, las enfermedades diarreicas agudas y las infecciones respiratorias agudas son las principales causas de asistencia hospitalaria en la provincia del Bayadh, ocasionado por el destete precoz y la desnutrición, factores que contribuyen a que la morbilidad tenga una alta incidencia y la mortalidad sea baja, por lo que se debe prestar especial atención a los pacientes menores de 5 años, entre ellos a los menores de un año, que son los que enferman con mayor frecuencia.

Introduction: Children represent the future, so their healthy growth and development should be a priority for all societies, that is the reason why the Health World Organization creates strategies to minimize the causes that conspire against the health of children and adolescents. Methods: A descriptive and longitudinal study of the 4 109 children over 28 days and under 14 years with infectious diseases was carried out, who were assisted in the Pediatrics Service of Mohamed Boudiaf Teaching Clinical Surgical Hospital, in the Bayadh province, Popular and Democratic Republic of Algeria, during 2017-2018. Objective: To characterize Algerian children with 28 days and under 14 years with infectious diseases from the clinical epidemiological point of view. Results: It was obtained that 88.4 % of the members in the study suffered from infectious diseases and 11.6 % suffered from non infectious diseases; equally, those under one year and from the male sex were the ones that mostly get ill (58.0 %), as long as, acute diarrheic diseases and breathing infections were the most frequent (48.0 and 32.0 %, respectively). Of the total of patients, 3 607 had a favorable clinical course (99.8 %). Conclusions: Infectious diseases constitute a health problem in Algeria that continues contributing to increase sick and dead children due to this cause; also, acute diarrheic diseases and acute breathing infections are the main causes of hospital assistance in Bayadh province, caused due to the early weaning and malnutrition, factors that contribute to the high incidence of morbidity and low mortality, reason why patients under 5 years should receive a special care, mainly those under one year who get sick with more frequency.

Ethnobotanical study of medicinal plants used for the treatment of Diabetes mellitus in Sidi Bel Abbes region (North-west Algeria) / Estudio etnobotánico de plantas medicinales utilizadas para el tratamiento de la diabetes mellitus en la región de Sidi Bel Abbes (noroeste de Argelia)

An ethnobotanical study was conducted with the aim to identify the medicinal plants used for the treatment of Diabetes mellitus (DM) in Sidi Bel Abbes region (Northwest Algeria). Sidi Bel Abbes, known for its diverse ecological habitats, such as mountains, steppe, lake, and fertile plains with diverse medicinal plants. The data was collected through questionnaire and interviews with inhabitants and traditional healers. The results obtained revealed that 33 plant species distributed in 20 genera belonging to 21 families for the treatment of DM were used. The most represented families were Lamiaceae, Asteraceae, Myrtaceae, Fabaceae and Lauraceae. Medicinal plants commonly used were Trigonella foenum-graecum, Olea europaea, Cinamomum cassia, Artemisia herba-alba, Lupinus albus, Juniperus communis, Rosmarinus officinalis, Prunus dulcis and Berberis vulgaris. The study revealed that, leaves, followed by seeds and stem bark were mostly used parts. Also, decoction and infusion were the most frequently used method of preparation. This study confirms that most people with Diabetes mellitus in the study areas rely on traditional medicine for their primary health care needs.

Se realizó un estudio etnobotánico con el objetivo de identificar las plantas medicinales utilizadas para el tratamiento de la Diabetes mellitus (DM) en la región de Sidi Bel Abbes (noroeste de Algeria). Sidi Bel Abbes, conocida por sus diversos hábitats ecológicos, como montañas, estepas, lagos y fértiles llanuras con plantas medicinales. Los datos se recopilaron mediante cuestionarios y entrevistas con habitantes y curanderos tradicionales. Los resultados obtenidos revelaron que 33 especies vegetales distribuidas en 20 géneros pertenecientes à 21 familias fueron utilizadas para el tratamiento de DM. Las familias más representadas fueron Lamiaceae, Asteraceae, Myrtaceae, Fabaceae y Lauraceae. Las plantas medicinales comúnmente utilizadas fueron Trigonella foenum-graecum, Olea europaea, Cinamomum cassia, Artemisia herba-alba, Lupinus albus, Juniperus communis, Rosmarinus officinalis, Prunus amygdalus y Berberis vulgaris. El estudio reveló que las hojas, seguidas de las semillas y la corteza de tallo, eran en su mayoría partes usadas. Sin embargo, la infusión y la decocción son el método de preparación más utilizado. El estudio reveló que las hojas, seguidas de las semillas y las partes del área, eran en su mayoría partes utilizadas. Tambien, la decocción y la infusión fueron los métodos de preparación más utilizado. Este estudio confirma que la mayoría de las personas con Diabetes mellitus en las áreas d'estudio dependen de la medicina tradicional para sus necesidades d'atenciónn primaria de la salud.

Antimicrobial and antibiofilm activities of phenolic compounds extracted from Populus nigra and Populus alba buds (Algeria)

The interest of this work is the discovery of new antimicrobial agents of plant origin to inhibit the formation of microbial biofilms. The present research was conducted to identify and quantify the phenolic compounds extracted from Populus nigra and Populus alba buds harvested in the area of Tizi-Ouzou (Algeria), and to evaluate their antimicrobial and antibiofilm activity. High performance liquid chromatography (HPLC) was performed to identify the phenolic compounds in the ethyl acetate fraction of P. nigra and the methanolic extracts of P. nigra and P. alba. The antimicrobial activity of the crude extracts and the fractions of these two species was tested against 11 microorganisms, using the disk diffusion method, while the antibiofilm effect of certain extracts was carried out in a 96-well microplate and on a biomaterial (catheter). HPLC analysis revealed the presence of 10 bioactive compounds. The main phenolic compounds identified in the three extracts were p-coumaric acid, ellagic acid, and Kaempferol. This study was able to demonstrate that the extracts of P. nigra and P. alba buds have interesting antimicrobial properties, with diameters ranging from 6.6 to 21.3 mm. In addition, extracts of P. nigra exhibited antibiofilm effects greater than 70%. Our results provide evidence for the antimicrobial and antibiofilm potential of bud extracts from both poplar species. Thus, these results will pave the way for further research on these two plants.

Primary antibiotic resistance and its relationship with cagA and vacA genes in Helicobacter pylori isolates from Algerian patients

Abstract The epidemiology of Helicobacter pylori resistance to antibiotics is poorly documented in Africa and especially in Algeria. The aim of our study was to determine the antibiotic resistance rates, as well as its possible relationship with VacA and CagA virulence markers of isolates from Algerian patients. One hundred and fifty one H. pylori isolate were obtained between 2012 and 2015 from 200 patients with upper abdominal pain. Antimicrobial susceptibility testing was performed for amoxicillin, clarithromycin, metronidazole, ciprofloxacin, rifampicin and tetracycline. Molecular identification of H. pylori and the detection of vacA and cagA genes were performed using specific primers. We found that H. pylori was present in 83.5% of collected biopsies, 54.9% of the samples were cagA positive, 49.67% were vacA s1m1, 18.30% were vacA s1m2 and 25.49% were vacA s2m2. Isolates were characterized by no resistance to amoxicillin (0%), tetracycline (0%), rifampicin (0%), a high rate of resistance to metronidazole (61.1%) and a lower rate of resistance to clarithromycin (22.8%) and ciprofloxacin (16.8%). No statically significant relationship was found between vagA and cagA genotypes and antibiotic resistance results (p > 0.5) except for the metronidazole, which had relation with the presence of cagA genotype (p = 0.001).

Desafios para o trabalho em saúde: um estudo comparado de Hospitais Universitários na Argélia, Brasil e França / Challenges for work in healthcare: comparative study on University Hospitals in Algeria, Brazil and France

Resumo O estudo compara os desafios no trabalho em serviços de emergência de hospitais universitários públicos na Argélia, Brasil e França. A descrição e a análise estão organizadas em três eixos: contexto e trajetória dos sistemas, hospitais e serviços de emergência, e os desafios enfrentados. Nos serviços foram feitas entrevistas, questionários, observação e "groupes de rencontre du travail"/GRT. Para o processo analítico utilizou-se técnicas do "participatory appraisal" complementadas com triangulação de fontes e dados. Os principais desafios referidos foram: déficit da força de trabalho; falta de leitos nos serviços de internação; déficit de infraestrutura e materiais; excesso de atividades cronofágicas; transição geracional; violência pelos usuários e familiares. Medidas de racionalização e contenção de gastos repercutem de modo mais intenso na Argélia e no Brasil onde há um baixo patamar de financiamento público. Nota-se que a gestão hospitalar não pode estar dissociada do planejamento da rede de atenção, tendo em vista as crescentes pressões do complexo produtivo, da transição demográfica e epidemiológica. A médio prazo, medidas que possam atenuar atividades cronofágicas, o deficit de materiais e a violência devem ser consideradas na melhoria do trabalho em emergências.

Abstract This study compares the challenges for work in emergency services of publicuniversity hospitals in Algeria, Brazil and France. The description and analysis are organized in three topics: context and trajectory of the health systems; hospitals and emergency services; and the challenges that are faced. The research carried out interviews, surveys, observation and "groupes de rencontre du travail" / GRT. The data analysis was done using participatory appraisal techniques associated to triangulation of sources and data. The main challenges found were: workforce deficit; lack of hospitals beds in inpatient units; deficit of infrastructure and materials; excess of "chronophagic activities"; generational transition; and violence by patients and families.Despite their particularities, the countries coincide regarding the challenges. Measures to rationalize and restrain spending have a greater impact on Algeria and Brazil due to the low level of public funding, but they also occur in France. The hospital management cannot be dissociated from healthcare system planningconsidering the increasing pressures of the demographic and epidemiological transition. In medium term, measures that may mitigate "chronophagic activities", materials deficit and the violence should be considered to improve work in emergencies.

Traductores argelinos en el Hospital Oftalmológico Amistad Argelia-Cuba. Apuntes históricos y evolución / Algerian translators in the Ophthalmological Hospital Amistad Algeria-Cuba. Historical notes and evolution

Se realizó un análisis cronológico de la historia de la labor de los traductores argelinos en el Hospital Oftalmológico Amistad Argelia-Cuba, El Oued, Argelia, desde su inauguración en agosto de 2015 hasta la actualidad, con el objetivo de divulgar las experiencias en este tipo de trabajo. Los datos se obtuvieron de entrevistas a traductores que laboran en la institución desde su puesta en marcha, los cuales aportaron además fotografías, lo que permitió, reunir la información necesaria para desarrollar este artículo. Se abordó la evolución del binomio médico-traductor, para garantizar una atención oftalmológica adecuada en la institución(AU)

A chronological analysis was done of the history of the work of the Algerian translators in the Ophthalmological Hospital Algeria-Cuba, El Oued, from its inauguration in August 2015 to the present, with the aim of disseminating the experiences in this type of work. The data was obtained from interviews with translators who work at the institution since its implementation, who also contributed photographs, for obtaining the necessary information to develop this article. The evolution of the doctor-translator binomial was addressed, in order to guarantee adequate ophthalmological care in the institution(AU)

Afecciones oculares de pacientes pediátricos en consulta de Neuroftalmología en Oued, Argelia / Doenças oculares de pacientes pediátricos em, consulta de Neuroptalmologia em Oued, Argélia / Ocular conditions in pediatric patients seen in Neurophthalmology clinic, Oued, Algeria

Introducción: cuando la visión está disminuida o ausente ocasiona minusvalías para los niños, y provoca lentitud en el aprendizaje. Objetivo: determinar las afecciones oculares de pacientes pediátricos en consulta de Neuroftalmología, Hospital Oftalmológico Amistad Argelia-Cuba, El Oued, en el período enero a junio de 2016. Método: se realizó un estudio observacional, descriptivo, el universo quedó constituido por 825 niños. Se estudiaron las variables: edad, sexo, diagnóstico oftalmológico y tipo de tratamiento recibido. Resultados: el 65,5 por ciento de pacientes correspondió al sexo masculino, la edad más representada estuvo entre 11-14 años para un 48,5 por ciento. La ambliopía refractiva y el estrabismo fueron las afecciones oftalmológicas más frecuentes, en el 60,6 y 15,8 por ciento de los casos respectivamente. Conclusiones: el trabajo del neuroftalmólogo y su equipo de salud, contribuye al tratamiento de la ambliopía, recibiendo la mayoría de los niños corrección óptica en consulta(AU)

Introduction: when vision is diminished or absent it causes disabilities for children and slow learning. Objective: to determine the ocular affections of pediatric patients in Neurophthalmology consultation at the ophthalmological Hospital Amistad-Algeria-Cuba, El Oued, from January to June 2016. Method: an observational and descriptive study was carried out; also the universe was constituted by 825 children. The variables were studied: age, sex, ophthalmological diagnosis and type of treatment received by children. Results: 65.5 percent of patients were males; the most represented age was between 11-14 years for 48.5 percent. Refractive amblyopia and strabismus were the most frequent ophthalmological affections, in 60.6 and 15.8 percent of cases respectively. Conclusions: the work of the neurophthalmologist and his health team contributes to the treatment of amblyopia receiving most of the children optical correction in consultation(AU)

Introdução: quando a visão é diminuída ou ausente, causa incapacidades para as crianças e causa um aprendizado lento. Objetivo: determinar as condições oculares de pacientes pediátricos em um Neuro-Oftalmologia, Eye Hospital Amizade Argélia-Cuba, El Oued, no período de janeiro a junho de 2016. Método: um estudo observacional, descritivo, o universo era composto por 825 crianças. As variáveis estudadas foram: idade, sexo, diagnóstico oftalmológico e tipo de tratamento recebido. Resultados: 65,5 por cento dos pacientes corresponderam ao sexo masculino, a idade mais representada foi entre 11-14 anos para 48,5 por cento. A ambliopia refrativa e o estrabismo foram as afecções oftalmológicas mais frequentes, em 60,6 e 15,8 por cento dos casos, espectivamente. Conclusões: o trabalho do neuroftalmologista e sua equipe de saúde contribuem para o tratamento da ambliopia, sendo que a maioria das crianças recebe correção óptica em consulta(AU)

Molecular characterization and epidemiology of cefoxitin resistance among Enterobacteriaceae lacking inducible chromosomal ampC genes from hospitalized and non-hospitalized patients in Algeria: description of new sequence type in Klebsiella pneumoniae isolates

In this study, 922 consecutive non-duplicate clinical isolates of Enterobacteriaceae obtained from hospitalized and non-hospitalized patients at Bejaia, Algeria were analyzed for AmpC-type β-lactamases production. The ampC genes and their genetic environment were characterized using polymerase chain reaction (PCR) and sequencing. Plasmid incompatibility groups were determined by using PCR-based replicon typing. Phylogenetic grouping and multilocus sequence typing were determined for molecular typing of the plasmid-mediated AmpC (pAmpC) isolates.Of the isolates, 15 (1.6%) were identified as AmpC producers including 14 CMY-4- producing isolates and one DHA-1-producing Klebsiella pneumoniae. All AmpC-producing isolates co-expressed the broad-spectrum TEM-1 β-lactamase and three of them co-produced CTX-M and/or SHV-12 ESBL. Phylogenetic grouping and virulence genotyping of the E. coli isolates revealed that most of them belonged to groups D and B1. Multilocus sequence typing analysis of K. pneumoniae isolates identified four different sequence types (STs) with two new sequences: ST1617 and ST1618. Plasmid replicon typing indicates that bla CMY-4 gene was located on broad host range A/C plasmid, while LVPK replicon was associated with bla DHA-1. All isolates carrying bla CMY-4 displayed the transposon-like structures ISEcp1/AISEcp1-blaCMY-blc-sugE. Our study showed that CMY-4 was the main pAmpC in the Enterobacteriaceae isolates inAlgeria.

Interruptions of nurses' activities and patient safety: an integrative literature review / Interrupções de atividades de enfermeiros e a segurança do paciente: revisão integrativa da literatura / Interrupciones de actividades de enfermeros y la seguridad del paciente: revisión integradora de la literatura

OBJECTIVES: to identify characteristics related to the interruption of nurses in professional practice, as well as to assess the implications of interruptions for patient safety. METHOD: integrative literature review. The following databases were searched: Pubmed/Medline, LILACS, SciELO and Cochrane Library, using the descriptors interruptions and patient safety. An initial date was not established, but the final date was December 31, 2013. A total of 29 papers met the inclusion criteria. RESULTS: all the papers included describe interruptions as a harmful factor for patient safety. Data analysis revealed three relevant categories: characteristics of interruptions, implications for patient safety, and interventions to minimize interruptions. CONCLUSION: interruptions favor the occurrence of errors in the health field. Therefore, there is a need for further studies to understand such a phenomenon and its effects on clinical practice. .

OBJETIVOS: identificar características relacionadas à interrupção de enfermeiros em sua prática profissional, bem como avaliar as implicações para a segurança do paciente. MÉTODO: foi realizada revisão de literatura do tipo integrativa, com busca nas bases de dados Pubmed/Medline, LILACS, SciELO e Biblioteca Cochrane, utilizando os descritores interruptions e patient safety. A data inicial não foi limitada e a data final foi 31 de dezembro de 2013, identificando-se 29 artigos que atenderam aos critérios de inclusão. RESULTADOS: todos os artigos revisados descreveram a interrupção como fator prejudicial à segurança do paciente. A análise destes estudos revelou três categorias relevantes: características da interrupção, implicações da interrupção para a segurança do paciente e intervenções para minimizar as interrupções. CONCLUSÃO: a interrupção favorece a ocorrência de erros na saúde. Assim, notou-se necessidade de novas pesquisas para compreender tal fenômeno e seus efeitos na prática clínica. .

OBJETIVOS: identificar características relacionadas a la interrupción que sufren los enfermeros en su práctica profesional, así como evaluar las implicaciones para la seguridad del paciente. MÉTODO: fue realizada una revisión de literatura de tipo integradora, con búsqueda en las bases de datos Pubmed/Medline, LILACS, SciELO y Biblioteca Cochrane, utilizando los descriptores interruptions y patient safety. La fecha inicial no fue limitada y la fecha final fue 31 de diciembre de 2013, se identificaron 29 artículos que atendieran a los criterios de inclusión. RESULTADOS: todos los artículos revisados describieron la interrupción como un factor perjudicial a la seguridad del paciente. El análisis de estos estudios reveló tres categorías relevantes: características de la interrupción, implicaciones de la interrupción para la seguridad del paciente e intervenciones para minimizar las interrupciones. CONCLUSIÓN: la interrupción favorece la ocurrencia de errores en la salud. Así, se notó la necesidad de realizar nuevas investigaciones para comprender ese fenómeno y los efectos del mismo en la práctica clínica. .

Binationalité en acte: une nouvelle condition politique / Binacionalidade em ação: um novo status político / Binationality in action: a new political status / Binacionalidad en acción: un nuevo status político

Cet article interroge les enjeux socio-politiques découlant de la possession de la nationalité française et algérienne, d’Algériens (nes) vivant en France. Les effets de la binationalité se rapportent aux changements de positionnement politique produits par cette nouvelle condition politique, aux usages publics construits par une partie des Franco-Algériens. L’interprétation de ces transformations s’appuie sur leurs discours publics, fondés sur une demande explicite de reconnaissance de leur double appartenance sociale, culturelle et de leur double légitimité politique, tant dans la sphère française qu’algérienne. La posture de ces acteurs émergents dans les deux espaces publics tend à contester les frontières établies par les configurations identitaires nationales.

Este artigo examina as questões político-sociais decorrentes da dupla cidadania franco-argelina de argelinos vivendo na França. Os efeitos da binacionalidade estão relacionados às mudanças políticas produzidas por este novo status político, bem como às formas pelas quais alguns franco-argelinos fazem uso público disso. Essas mudanças podem ser entendidas a partir dos posicionamentos públicos destes cidadãos, os quais são embasados numa demanda explícita de legitimidade em ambas as esferas, francesa e argelina. As posições assumidas por estes atores emergentes em ambas as esferas públicas desafia as fronteiras estabelecidas pelas configurações identitárias nacionais.

This article examines the socio-political issues arising from the dual French-Algerian citizenship of Algerians living in France. The effects of binationality is related to policy changes produced by this new political status as well as to the ways in which some Franco-Algerians make public use of it. These changes can be understood from their public statements, which are based on an explicit request for recognition of their dual social-cultural membership and on their dual political legitimacy in both the French and Algerian spheres. The stances assumed by these emerging actors in both public areas challenge the boundaries established by nationalist constituencies.

Este artículo examina las cuestiones político-sociales debidas de la doble ciudadanía franco-argelina de argelinos viviendo en la Francia. Los efectos de la binacionalidad están relacionados a los cambios políticos producidos por este nuevo status político, como a las maneras que algunos franco-argelinos hacen uso público de eso. Estos cambios pueden ser entendidos a partir de los posicionamientos públicos de estos ciudadanos, los cuales son embasados en una demanda explícita de legitimidad en ambas las esferas, la francesa y la argelina. Las posiciones asumidas por estos actores emergentes en ambas las esferas públicas desafían las fronteras establecidas por las configuraciones de identidades nacionales.

Limits of a rapid identification of common Mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism

A total of 131 phlebotomine Algerian sandflies have been processed in the present study. They belong to the species Phlebotomus bergeroti, Phlebotomus alexandri, Phlebotomus sergenti, Phlebotomus chabaudi, Phlebotomus riouxi, Phlebotomus perniciosus, Phlebotomus longicuspis, Phlebotomus perfiliewi, Phlebotomus ariasi, Phlebotomus chadlii, Sergentomyia fallax, Sergentomyia minuta, Sergentomyia antennata, Sergentomyia schwetzi, Sergentomyia clydei, Sergentomyia christophersi and Grassomyia dreyfussi. They have been characterised by sequencing of a part of the cytochrome b (cyt b), t RNA serine and NADH1 on the one hand and of the cytochrome C oxidase I of the mitochondrial DNA (mtDNA) on the other hand. Our study highlights two sympatric populations within P. sergenti in the area of its type-locality and new haplotypes of P. perniciosus and P. longicuspis without recording the specimens called lcx previously found in North Africa. We tried to use a polymerase chain reaction-restriction fragment length polymorphism method based on a combined double digestion of each marker. These method is not interesting to identify sandflies all over the Mediterranean Basin.