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1.
Food Chem ; 462: 141003, 2025 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-39208735

RESUMO

Recently, the increasing incidence of malignant melanoma has become a major public health concern owing to its poor prognosis and impact on quality of life. Consuming foods with potent antitumor compounds can help prevent melanoma and maintain skin health. Fucoxanthin (FX), a naturally occurring carotenoid found in brown algae, possesses antitumor properties. However, its bioavailability, safety risks, and in vivo effects and mechanisms against melanoma remain unclear. This research focused on evaluating the safety and prospective antimelanoma impact of simulated gastrointestinal digestion products (FX-ID) on HaCaT and A375 cells.The results indicate that FX-ID exerts negative effects on mitochondria in A375 cells, increases Bax expression, releases Cytochrome C, and activates cleaved caspase-3, ultimately promoting apoptosis. Additionally, FX-ID influences the mitogen-activated protein kinase (MAPK) pathway by enhancing cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-κB) levels, consequently facilitating apoptosis and inflammation without significantly impacting HaCaT cells. These findings provide insight into inhibitory mechanism of FX-ID against melanoma, guiding the development of functional foods for prevention.


Assuntos
Apoptose , Queratinócitos , Melanoma , Xantofilas , Humanos , Melanoma/metabolismo , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Apoptose/efeitos dos fármacos , Xantofilas/farmacologia , Xantofilas/química , Linhagem Celular Tumoral , NF-kappa B/metabolismo , NF-kappa B/genética , Digestão , Modelos Biológicos , Ciclo-Oxigenase 2/metabolismo , Ciclo-Oxigenase 2/genética , Antineoplásicos/farmacologia , Antineoplásicos/química , Phaeophyceae/química , Mitocôndrias/metabolismo , Mitocôndrias/efeitos dos fármacos , Caspase 3/metabolismo , Caspase 3/genética
2.
J Oleo Sci ; 73(9): 1177-1187, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39218637

RESUMO

In this paper, the lipid classes, compositions of the neutral lipids, phospholipids and fatty acids, acetylcholinesterase inhibition and cytotoxic activity of two brown algae Lobophora tsengii D. Tien & Z. Sun and Lobophora australis Z. Sun, F. C. Gurgel & H. Kawai have been investigated. The polar lipid class had the highest content in total lipid (TL) (43.47% in L. tsengii and 48.95% in L. australis). Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were the main components in the phospholipids of two studied brown algae with contents varied from 32.27% to 52.33%. Total lipids were rich in PUFA (42.54% of total fatty acids for L. australis and 32.98% for L. tsengii), with EPA (11.46%, 14.30%) and AA (8.0%, 11.96%). L. tsengii methanol extract inhibited acetylcholinesterase (AChE) in in vitro assay with an IC50 value of 25.45 µg/mL. Both Lobophora methanol extracts display cytotoxic effects against four human cancer cell lines (KB, MCF7, HepG2 and A549) with IC50 in the range of 21.11-83.61 µg/mL. Especially, L. australis extract showed a strong cytotoxicity against KB cell lines with IC50 value of 21.11±0.39 µg/mL.


Assuntos
Acetilcolinesterase , Inibidores da Colinesterase , Phaeophyceae , Phaeophyceae/química , Inibidores da Colinesterase/farmacologia , Inibidores da Colinesterase/isolamento & purificação , Humanos , Acetilcolinesterase/metabolismo , Fosfolipídeos , Lipídeos , Ácidos Graxos/análise , Fosfatidiletanolaminas , Linhagem Celular Tumoral , Fosfatidilcolinas , Antineoplásicos/farmacologia , Antineoplásicos/isolamento & purificação , Antineoplásicos/química , Ácidos Graxos Insaturados/farmacologia
3.
Development ; 151(20)2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-39190296

RESUMO

Brown algae are multicellular photosynthetic organisms that have evolved independently of plants and other algae. Here, we have studied the determinism of body axis formation in the kelp Saccharina latissima. After microdissection of the embryo, we show that the stalk, an empty cell that retains the embryo on the maternal tissue, represses longitudinal cell divisions in the early embryo, thereby reinforcing the establishment of the initial apico-basal axis. In addition, it promotes cell growth and controls cell shape and arrangement in the flat oblong embryo composed of cells aligned in rows and columns. Although the stalk persists for several weeks until the embryo reaches at least 500 cells, proper embryogenesis requires connection to maternal tissue only during the first 4 days after fertilisation, i.e. before the embryo reaches the 8-cell stage. Transplantation experiments indicate that the maternal signal is not diffused in seawater, but requires contact between the embryo and the maternal tissue. This first global quantitative study of brown algal embryogenesis highlights the role of MUM, an unknown maternal message, in the control of growth axes and tissue patterning in kelp embryos.


Assuntos
Kelp , Padronização Corporal/fisiologia , Regulação da Expressão Gênica no Desenvolvimento , Phaeophyceae , Proteínas de Algas/metabolismo , Algas Comestíveis , Laminaria
4.
Artigo em Inglês | MEDLINE | ID: mdl-39140846

RESUMO

Two Gram-negative, obligately aerobic, rod-shaped bacteria, strains G1-22T and G1-23T, were isolated from the phycosphere of a marine brown alga. Both strains exhibited catalase- and oxidase-positive activities. Strain G1-22T displayed optimal growth at 25 °C, pH 8.0, and 2.0-3.0% (w/v) NaCl, while strain G1-23T exhibited optimal growth at 25 °C, pH 8.0, and 4.0% NaCl. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. As major fatty acids (> 5%), strain G1-22T contained C16 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c), C12 : 1 3-OH, and C10 : 0 3-OH, while strain G1-23T contained C16 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), and C14 : 0. Phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were major polar lipids in both strains. Strains G1-22T and G1-23T had DNA G+C contents of 40.2 and 38.9 mol%, respectively. Phylogenetic analyses based on 16S rRNA and genome sequences revealed that strains G1-22T and G1-23T formed distinct phylogenetic lineages within the genera Psychrosphaera and Paraglaciecola, respectively. Strain G1-22T showed closest relatedness to Psychrosphaera ytuae MTZ26T with 97.8% 16S rRNA gene sequence similarity, 70.2% average nucleotide identity (ANI), and a 21.5% digital DNA-DNA hybridization (dDDH) value, while strain G1-23T was most closely related to Paraglaciecola aquimarina KCTC 32108T with 95.6% 16S rRNA gene sequence similarity, 74.6% ANI, and a 20.1% dDDH value. Based on phenotypic and molecular characteristics, strains G1-22T and G1-23T are proposed to represent two novel species, namely Psychrosphaera algicola sp. nov. (type strain G1-22T=KACC 22486T=JCM 34971T) and Paraglaciecola algarum sp. nov. (type strain G1-23T=KACC 22490T=JCM 34972T), respectively. Additionally, based on the comparison of whole genome sequences, it is proposed that Pseudoalteromonas elyakovii, Pseudoalteromonas flavipulchra, and Pseudoalteromonas profundi are reclassified as later heterotypic synonyms of Pseudoalteromonas distincta, Pseudoalteromonas maricaloris, and Pseudoalteromonas gelatinilytica, respectively.


Assuntos
Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano , Ácidos Graxos , Hibridização de Ácido Nucleico , Filogenia , Pseudoalteromonas , RNA Ribossômico 16S , Análise de Sequência de DNA , Ubiquinona , RNA Ribossômico 16S/genética , DNA Bacteriano/genética , Pseudoalteromonas/genética , Pseudoalteromonas/classificação , Pseudoalteromonas/isolamento & purificação , Phaeophyceae/microbiologia
5.
Int J Mol Sci ; 25(16)2024 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-39201271

RESUMO

Sargassum hemiphyllum var. chinense, a prevalent seaweed along the Chinese coast, has economic and ecological significance. However, systematic positions within Sargassum and among the three orders of Phaeophyceae, Fucales, Ectocarpales, and Laminariales are in debate. Here, we reported the organellar genomes of S. hemiphyllum var. chinense (34,686-bp mitogenome with 65 genes and 124,323 bp plastome with 173 genes) and the investigation of comparative genomics and systematics of 37 mitogenomes and 22 plastomes of Fucales (including S. hemiphyllum var. chinense), Ectocarpales, and Laminariales in Phaeophyceae. Whole genome collinearity analysis showed gene number, type, and arrangement were consistent in organellar genomes of Sargassum with 360 SNP loci identified as S. hemiphyllum var. chinense and two genes (rps7 and cox2) identified as intrageneric classifications of Sargassum. Comparative genomics of the three orders of Phaeophyceae exhibited the same content and different types (petL was only found in plastomes of the order Fucales and Ectocarpales) and arrangements (most plastomes were rearranged, but trnA and trnD in the mitogenome represented different orders) in genes. We quantified the frequency of RNA-editing (canonical C-to-U) in both organellar genomes; the proportion of edited sites corresponded to 0.02% of the plastome and 0.23% of the mitogenome (in reference to the total genome) of S. hemiphyllum var. chinense. The repetition ratio of Fucales was relatively low, with scattered and tandem repeats (nine tandem repeats of 14-24 bp) dominating, while most protein-coding genes underwent negative selection (Ka/Ks < 1). Collectively, these findings provide valuable insights to guide future species identification and evolutionary status of three important Phaeophyceae order species.


Assuntos
Genoma Mitocondrial , Filogenia , Sargassum , Sargassum/genética , Sargassum/classificação , Genoma Mitocondrial/genética , Genômica/métodos , Phaeophyceae/genética , Phaeophyceae/classificação , Evolução Molecular
6.
Physiol Plant ; 176(4): e14503, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39191702

RESUMO

Worldwide, where the demand for novel and greener solutions for sustainable agricultural production is increasing, the use of eco-friendly products such as seaweed-derived biostimulants as pre-sowing treatment represent a promising and important approach for the future. Cystoseira barbata, a brown seaweed species abundant in the Mediterranean Region, was collected from the Marmara Sea and subjected to water, alkali, and acidic extractions, and the biostimulant activity of these extracts was tested on wheat (Triticum durum cv. Saricanak-98) using different rates through application to the seeds or germination medium (substrate) applications. The different extracts were characterized by mineral, total phenolic, free amino acid, mannitol, polysaccharide, antioxidant concentrations and hormone-like activity. The effects of the extracts on growth parameters, root morphology, esterase activity, and mineral nutrient concentrations of wheat seedlings were investigated. Our results suggest that the substrate application was more effective in enhancing the seedling performance compared to the seed treatment. High rates of seaweed extracts applied to substrates increased the shoot length and fresh weight of wheat seedlings by up to 20 and 25%, respectively. The substrate applications enhanced the root fresh weights of wheat seedlings by up to 25% when compared to control plants. Among the biostimulant extract applications, the water extract at the highest rate yielded the most promising results in terms of the measured parameters. Cystoseira barbata extracts with different compositions can be used as effective biostimulants to boost seedling growth. The local seaweed biomass affected by mucilage problems, has great potential as a bioeconomy resource and can contribute to sustainable practices for agriculture.


Assuntos
Alga Marinha , Plântula , Triticum , Triticum/crescimento & desenvolvimento , Triticum/efeitos dos fármacos , Triticum/fisiologia , Plântula/efeitos dos fármacos , Plântula/crescimento & desenvolvimento , Alga Marinha/crescimento & desenvolvimento , Germinação/efeitos dos fármacos , Phaeophyceae/crescimento & desenvolvimento , Phaeophyceae/fisiologia , Phaeophyceae/efeitos dos fármacos , Antioxidantes/metabolismo , Sementes/efeitos dos fármacos , Sementes/crescimento & desenvolvimento , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/efeitos dos fármacos , Extratos Vegetais/farmacologia
7.
Mar Drugs ; 22(8)2024 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-39195446

RESUMO

This study explores the anti-obesity effects of the ethyl acetate extract of Ecklonia cava (EC-ETAC) on 3T3-L1 preadipocytes, focusing on its impact on adipogenesis, lipolysis, and adipose browning via the HO-1/Nrf2 pathway. Western blot analysis revealed that EC-ETAC significantly inhibited adipogenic transcription factors (PPARγ, C/EBPα, SREBP-1) and lipogenesis-related proteins (FAS, LPL). Concurrently, EC-ETAC enhanced lipolytic markers (p-AMPK, p-HSL) and adipose browning-related proteins (UCP-1, PGC-1α), indicating its role in promoting lipolysis and adipose browning. The inhibition of HO-1 by zinc protoporphyrin (ZnPP) significantly reversed these effects, underscoring the critical role of HO-1 in mediating the anti-obesity properties of EC-ETAC. Additionally, fluorescence measurements and Oil Red O staining confirmed the reduction of lipid accumulation and oxidative stress upon EC-ETAC treatment. These findings suggest that EC-ETAC exerts its anti-obesity effects by modulating the HO-1/Nrf2 pathway, which is crucial for regulating adipogenesis, lipolysis, and adipose browning. This study highlights the potential of EC-ETAC as a natural therapeutic agent for obesity management and supports further research into its clinical applications. By targeting the HO-1/Nrf2 pathway, EC-ETAC could offer a novel approach to enhancing energy expenditure and reducing fat mass, thereby improving metabolic health.


Assuntos
Células 3T3-L1 , Adipócitos , Adipogenia , Fármacos Antiobesidade , Heme Oxigenase-1 , Fator 2 Relacionado a NF-E2 , Phaeophyceae , Transdução de Sinais , Animais , Camundongos , Adipogenia/efeitos dos fármacos , Fator 2 Relacionado a NF-E2/metabolismo , Phaeophyceae/química , Transdução de Sinais/efeitos dos fármacos , Adipócitos/efeitos dos fármacos , Adipócitos/metabolismo , Heme Oxigenase-1/metabolismo , Fármacos Antiobesidade/farmacologia , Lipólise/efeitos dos fármacos , Extratos Vegetais/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Obesidade/tratamento farmacológico , Obesidade/metabolismo , Proteínas de Membrana
8.
Mar Drugs ; 22(8)2024 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-39195456

RESUMO

This study explores the potential of producing bioethanol from seaweed biomass and reusing the residues as antioxidant compounds. Various types of seaweed, including red (Gelidium amansii, Gloiopeltis furcata, Pyropia tenera), brown (Saccharina japonica, Undaria pinnatifida, Ascophyllum nodosum), and green species (Ulva intestinalis, Ulva prolifera, Codium fragile), were pretreated with dilute acid and enzymes and subsequently processed to produce bioethanol with Saccharomyces cerevisiae BY4741. Ethanol production followed the utilization of sugars, resulting in the highest yields from red algae > brown algae > green algae due to their high carbohydrate content. The residual biomass was extracted with water, ethanol, or methanol to evaluate its antioxidant activity. Among the nine seaweeds, the A. nodosum bioethanol residue extract (BRE) showed the highest antioxidant activity regarding the 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity, ferric reducing antioxidant power (FRAP), and reactive oxygen species (ROS) inhibition of H2O2-treated RAW 264.7 cells. These by-products can be valorized, contributing to a more sustainable and economically viable biorefinery process. This dual approach not only enhances the utilization of marine resources but also supports the development of high-value bioproducts.


Assuntos
Antioxidantes , Biomassa , Etanol , Saccharomyces cerevisiae , Alga Marinha , Alga Marinha/química , Alga Marinha/metabolismo , Antioxidantes/farmacologia , Antioxidantes/química , Animais , Camundongos , Saccharomyces cerevisiae/metabolismo , Células RAW 264.7 , Biocombustíveis , Espécies Reativas de Oxigênio/metabolismo , Rodófitas/química , Rodófitas/metabolismo , Phaeophyceae/química
9.
Mar Drugs ; 22(8)2024 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-39195459

RESUMO

Acne is one of the most common dermatological conditions, peaking during adolescence and early adulthood, affecting about 85% of individuals aged 12-24. Although often associated with teenage years, acne can occur at any age, impacting over 25% of women and 12% of men in their forties. Treatment strategies vary depending on the severity, including the use of topical gels or creams containing benzoyl peroxide and retinoids, antibiotics, and systemic or topical isotretinoin. However, these treatments can cause irritation, allergies, and other toxic side effects. Currently, there is no natural-based alternative for antibacterial photodynamic therapy targeting acne using marine drugs or extracts. Through a bioguided screening approach, we identified the ethanol extract of Skeletonema marinoi as highly phototoxic against three bacterial species associated with acne-Cutibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis. This extract exhibited phototoxicity in planktonic bacteria under white and red light, disrupted bacterial biofilms, reduced sebum production but also showed phototoxicity in keratinocytes, highlighting the importance of the specific targeting of treatment areas. Further investigations, including fractionation and high-resolution structural analysis, linked the observed phototoxicity to a high concentration of pheophorbide a in the extract. Given its notable in vitro efficacy, this extract holds promising potential for clinical evaluation to manage mild acne. This discovery paves the way for further exploration of Skeletonema pigment extracts, extending their potential applications beyond acne phototherapy to include dermocosmetics, veterinary medicine, and other phototherapy uses.


Assuntos
Acne Vulgar , Staphylococcus epidermidis , Acne Vulgar/tratamento farmacológico , Acne Vulgar/microbiologia , Humanos , Staphylococcus epidermidis/efeitos dos fármacos , Antibacterianos/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Etanol/química , Propionibacteriaceae/efeitos dos fármacos , Fotoquimioterapia/métodos , Phaeophyceae/química , Queratinócitos/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Feminino
10.
Mar Drugs ; 22(8)2024 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-39195470

RESUMO

Atopic dermatitis (AD) is a chronic skin condition that is characterized by dysregulated immune responses and a heightened risk of Staphylococcus aureus infections, necessitating the advancement of innovative therapeutic methods. This study explored the potential of (6Z,9Z,12Z,15Z)-(2R,3R,4R,5R)-2,3,4,5,6-pentahydroxyhexyl octadeca-6,9,12,15-tetraenoate (HSN-S1), a compound derived from the marine alga Hizikia fusiformis, which shows anti-inflammatory, antimicrobial, and immunomodulatory properties. HSN-S1 was isolated and characterized using advanced chromatographic and spectroscopic methods. Its efficacy was evaluated via in vitro assays with keratinocytes, macrophages, and T cells to assess cytokine suppression and its immunomodulatory effects; its antibacterial activity against S. aureus was quantified. The in vivo effectiveness was validated using a 2,4-dinitrochlorobenzene-induced AD mouse model that focused on skin pathology and cytokine modulation. HSN-S1 significantly reduced pro-inflammatory cytokine secretion, altered T-helper cell cytokine profiles, and showed strong antibacterial activity against S. aureus. In vivo, HSN-S1 alleviated AD-like symptoms in mice and reduced skin inflammation, transepidermal water loss, serum immunoglobulin-E levels, and Th2/Th17 cytokine outputs. These findings suggest HSN-S1 to be a promising marine-derived candidate for AD treatment, as it offers a dual-target approach that could overcome the limitations of existing therapies, hence warranting further clinical investigation.


Assuntos
Antibacterianos , Citocinas , Dermatite Atópica , Imunossupressores , Phaeophyceae , Staphylococcus aureus , Dermatite Atópica/tratamento farmacológico , Animais , Camundongos , Phaeophyceae/química , Antibacterianos/farmacologia , Antibacterianos/isolamento & purificação , Staphylococcus aureus/efeitos dos fármacos , Citocinas/metabolismo , Humanos , Imunossupressores/farmacologia , Imunossupressores/isolamento & purificação , Imunossupressores/química , Modelos Animais de Doenças , Ésteres/farmacologia , Ésteres/química , Feminino , Camundongos Endogâmicos BALB C , Organismos Aquáticos , Queratinócitos/efeitos dos fármacos
11.
Mar Drugs ; 22(8)2024 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-39195471

RESUMO

Due to the developing resistance and intolerance to antiretroviral drugs, there is an urgent demand for alternative agents that can suppress the viral load in people living with human immunodeficiency virus (HIV). Recently, there has been increased interest in agents of marine origin such as, in particular, fucoidans to suppress HIV replication. In the present study, the anti-HIV-1 activity of fucoidans from the brown algae Alaria marginata, Alaria ochotensis, Laminaria longipes, Saccharina cichorioides, Saccharina gurianovae, and Tauya basicrassa was studied in vitro. The studied compounds were found to be able to inhibit HIV-1 replication at different stages of the virus life cycle. Herewith, all fucoidans exhibited significant antiviral activity by affecting the early stages of the virus-cell interaction. The fucoidan from Saccharina cichorioides showed the highest virus-inhibitory activity by blocking the virus' attachment to and entry into the host's cell, with a selectivity index (SI) > 160.


Assuntos
Fármacos Anti-HIV , HIV-1 , Phaeophyceae , Polissacarídeos , Replicação Viral , Phaeophyceae/química , HIV-1/efeitos dos fármacos , Fármacos Anti-HIV/farmacologia , Polissacarídeos/farmacologia , Humanos , Replicação Viral/efeitos dos fármacos , Infecções por HIV/tratamento farmacológico , Infecções por HIV/virologia
12.
Carbohydr Polym ; 343: 122448, 2024 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-39174088

RESUMO

Fucoidans, a group of high molecular weight polysaccharides derived mainly from brown algae, are characterized by their high fucose content, degree of sulfation (DS), and intra- and interspecific structural variation. Fucoidans are increasingly recognized due to various reported bioactivities, potentially beneficial for human health. To unlock their potential use within biomedical applications, a better understanding of their structure-functional relationship is needed. To achieve this, systematic bioactivity studies based on well-defined, pure fucoidans, and the establishment of standardized, satisfactory purification protocols are required. We performed a comprehensive compositional and structural characterization of crude and ultra-purified fucoidans from three kelps: Saccharina latissima (SL), Alaria esculenta (AE) and Laminaria hyperborea (LH). Further, the complement-inhibiting activity of the purified fucoidans was assessed in a human whole blood model. The purification process led to fucoidans with higher DS and fucose and lower concentrations of other monosaccharides. Fucoidans from SL and LH resembles homofucans, while AE is a heterofucan rich in galactose with comparably lower DS. Fucoidans from SL and LH showed complement-inhibiting activity in blood and blood plasma, while no inhibition was observed for AE under the same conditions. The results emphasize the importance of high DS and possibly fucose content for fucoidans' bioactive properties.


Assuntos
Algas Comestíveis , Kelp , Laminaria , Phaeophyceae , Polissacarídeos , Humanos , Inativadores do Complemento/química , Inativadores do Complemento/isolamento & purificação , Inativadores do Complemento/farmacologia , Algas Comestíveis/química , Fucose/química , Fatores Imunológicos/farmacologia , Fatores Imunológicos/química , Fatores Imunológicos/isolamento & purificação , Kelp/química , Laminaria/química , Phaeophyceae/química , Polissacarídeos/química , Polissacarídeos/farmacologia , Polissacarídeos/isolamento & purificação , Água/química
13.
Elife ; 132024 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-39207920

RESUMO

Viruses are ubiquitous in nature and play key roles in various ecosystems. Notably, some viruses (e.g. bacteriophage) exhibit alternative life cycles, such as chronic infections without cell lysis. However, the impact of chronic infections and their interactions with the host organisms remains largely unknown. Here, we found for the first time that polysaccharides induced the production of multiple temperate phages infecting two deep-sea Lentisphaerae strains (WC36 and zth2). Through physiological assays, genomic analysis, and transcriptomics assays, we found these bacteriophages were released via a chronic style without host cell lysis, which might reprogram host polysaccharide metabolism through the potential auxiliary metabolic genes. The findings presented here, together with recent discoveries made on the reprogramming of host energy-generating metabolisms by chronic bacteriophages, shed light on the poorly explored marine virus-host interaction and bring us closer to understanding the potential role of chronic viruses in marine ecosystems.


Assuntos
Bacteriófagos , Polissacarídeos , Bacteriófagos/fisiologia , Bacteriófagos/genética , Polissacarídeos/metabolismo , Phaeophyceae/virologia , Água do Mar/virologia , Água do Mar/química , Interações entre Hospedeiro e Microrganismos
14.
Sci Rep ; 14(1): 20255, 2024 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-39215065

RESUMO

Cancer is a global issue and hence various efforts are being made. Iron oxide is considered a significant biochemical agent in the biomedical arena for cancer treatment. Marine macroalgae-mediated iron oxides especially, magnetite (Fe3O4) nanoparticles (NPs) are a prospective alternative to diagnose and treat cancer owing to their fluorescent and magnetic properties. We intend to appraise the usability of the aqueous extract of Rosenvingea intricata (R. intricata) in Fe3O4 NPs synthesis and to study their cytotoxic effects against human hepatocarcinoma (Hep3B) and pancreatic (PANC1) cancer cells. In the present study, R. intricata were collected from the coastal region of South Andaman, India. Aqueous extracts of R. intricata were utilized to synthesize Fe3O4 NPs via the co-precipitation method. Phycosynthesized Fe3O4 NPs exhibited wide peak at 400-600 nm from ultraviolet-visible diffused reflectance spectroscopic analysis which validated the formation of NPs. Band edge emission peak at 660 nm in fluorescent spectra confirmed the quantum confinement in Fe3O4 NPs. Fourier transform infrared spectroscopy confirmed the role of R. intricata as a capping and reducing agent with functional groups such as O-H, C-H, C=O, N=O, C=C, C-O, C-N, and C-S arising from amino acids, polysaccharides, aliphatic hydrocarbons, esters, amides, lignins, alkanes, aliphatic amines, and sulfates. Physicochemical properties such as crystallite size (14.36 nm), hydrodynamic size (84.6 nm), irregular morphology, elemental composition, particle size (125 nm), crystallinity, and saturation magnetization (0.90007 emu/g) were obtained from x-ray diffractometer, dynamic light scattering, scanning electron microscopy, energy dispersive x-ray spectrometer, high-resolution transmission electron microscopy, selected area electron diffraction and vibrating sample magnetometer techniques, respectively. The cell viability showed dose-dependent cytotoxic effects and enhanced the apoptosis against Hep3B and PANC1 cancer cells. R. intricata extract capped Fe3O4 NPs could be the most appropriate and effective nanomaterial for cancer treatment and management.


Assuntos
Antineoplásicos , Nanopartículas de Magnetita , Alga Marinha , Humanos , Alga Marinha/química , Índia , Nanopartículas de Magnetita/química , Linhagem Celular Tumoral , Antineoplásicos/farmacologia , Antineoplásicos/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Phaeophyceae/química , Sobrevivência Celular/efeitos dos fármacos
15.
Int J Biol Macromol ; 277(Pt 3): 134490, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39111494

RESUMO

Fucoidan is a sulfated polysaccharide that occurs naturally in the cell wall of brown seaweeds and has substantial biological efficacy. Optimizing the extraction of fucoidan from different brown seaweeds was the primary goal of this research. The optimization of fucoidan extraction was applied on the brown macroalga Turbinaria turbinata using a Box-Behnken Design (BBD) to inspect the impacts of different pH (3, 5, 7), temperature (70, 80, 90 °C) and extraction duration (60, 120, 180 min) on both the yield and sulfate content of fucoidan. The optimized parameters recorded to maximize the fucoidan yield and its sulfate content were a pH of 3.44 and a temperature of 82.26 °C for 60 min. The optimal conditions obtained from BBD were used for fucoidan extraction from T. turbinata, Sargassum cinereum, Padina pavonica, and Dictyota dichotoma. The highest average of fucoidan yield was derived from P. pavonica (40.76 ± 4.04 % DW). FTIR, 1H NMR, and HPLC were used to characterize extracted fucoidan. The extracted fucoidan's Physical characteristics, biochemical composition, antioxidant potential, antitumor effect against breast cancer cells (MCF-7), and antimicrobial and anticoagulant activity were assessed. The extracted fucoidan from D. dichotoma, followed by that extracted from S. cinereum, which had the highest sulphate content, depicted the highest antioxidant, anticancer, and anticoagulant activities. Fucoidan has demonstrated a strong antimicrobial action against some pathogenic microorganisms; Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, and Candida albicans. The anticoagulant properties of fucoidan from D. dichotoma were stronger than those of fucoidan from S. cinereum, T. turbinata, and P. pavonica due to its higher sulphate content. These findings could be used for various biomedical applications to improve the pharmaceutical industry.


Assuntos
Polissacarídeos , Polissacarídeos/farmacologia , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Humanos , Antineoplásicos/farmacologia , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Antioxidantes/farmacologia , Antioxidantes/química , Antioxidantes/isolamento & purificação , Células MCF-7 , Concentração de Íons de Hidrogênio , Temperatura , Alga Marinha/química , Antibacterianos/farmacologia , Antibacterianos/química , Antibacterianos/isolamento & purificação , Phaeophyceae/química , Testes de Sensibilidade Microbiana , Anticoagulantes/farmacologia , Anticoagulantes/química , Anticoagulantes/isolamento & purificação
16.
BMC Vet Res ; 20(1): 281, 2024 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-38951863

RESUMO

The aim of this research was to estimate the immunopotentiation effect of brown algae Padina boergesenii water extract on Nile tilapia, Oreochromis niloticus through resistance to Pseudomonas putida infection. Gas Chromatography Mass Spectrometry was utilized to characterize the seaweed phytoconstituents. One hundred and twenty-six fish were divided in triplicates into two equal groups corresponding to two diet variants that used to feed Nile tilapia for 20 successive days: a basal (control), and P. boergesenii water extract supplemented group. Fish samples were collected at 10-days intervals throughout the experiment. Serum biochemical constituents, total antioxidant capacity (TAC), and some immune related genes expression of the spleen and intestinal tissues of experimental fish were studied, as well as histological examination of fish immune tissues. Moreover, following 20 days of feeding, the susceptibility of Nile tilapia to P. putida infection was evaluated to assess the protective effect of the used extract. The findings indicated that the studied parameters were significantly increased, and the best immune response profiles were observed in fish fed P. boergesenii water extract for 20 successive days. A bacterial challenge experiment using P. putida resulted in higher survival within the supplemented fish group than the control. Thus, the lowered post-challenge mortality of the fish may be related to the protection provided by the stimulation of the innate immune system, reduced oxidative stress by higher activity of TAC, and elevated levels of expression of iterleukin-1beta (IL-1ß), beta-defensin (ß-defensin), and natural killer-lysin (NKl). Moreover, the constituents of the extract used showed potential protective activity for histological features of the supplemented fish group when compared to the control. Collectively, this study presents a great insight on the protective role of P. boergesenii water extract as an additive in Nile tilapia feed which suggests its potential for improving the immune response against P. putida infection.


Assuntos
Ração Animal , Ciclídeos , Suplementos Nutricionais , Doenças dos Peixes , Infecções por Pseudomonas , Pseudomonas putida , Animais , Pseudomonas putida/efeitos dos fármacos , Doenças dos Peixes/microbiologia , Doenças dos Peixes/prevenção & controle , Ração Animal/análise , Infecções por Pseudomonas/veterinária , Infecções por Pseudomonas/tratamento farmacológico , Phaeophyceae/química , Dieta/veterinária , Resistência à Doença/efeitos dos fármacos , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Extratos Vegetais/administração & dosagem
17.
Carbohydr Polym ; 342: 122302, 2024 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-39048211

RESUMO

Methylation followed by depolymerization and gas chromatography (GC) is an effective methodology for the linkage analysis of polysaccharides, including fucoidan, a sulphated algal polysaccharide. However, this sample material demands attention to experimental details to prevent aberrations in the analytical result. The use of deficient bases for methylation, the presence of water, analyte degradation during hydrolysis, and coelution of the target analytes during gas chromatography create doubts about published results. We therefore investigated critical parameters of the method and carefully optimized the steps of the protocol to ensure the integrity of the results for the fucose monomers. Fucoidan from Cladosiphon okamuranus was used as reference sample to determine the glycosidic bonds, and sulphate positions in the monomer. Fucoidan in protonated form was methylated in a strictly water-free environment using lithium dimsyl as base and methyl iodide for methylation. The methylated polymer was isolated by solid phase extraction, which was crucial to recover also the highly sulfated fraction. Hydrolysis was conducted with trifluoroacetic acid. To separate all target analytes in GC-FID/MS, a stationary phase with high cyanopropyl content (HP-88) was required, as the commonly employed phenyl siloxane phases result in co-elution, which distorts the result severely.


Assuntos
Fucose , Phaeophyceae , Polissacarídeos , Polissacarídeos/química , Fucose/química , Metilação , Phaeophyceae/química , Hidrólise , Cromatografia Gasosa-Espectrometria de Massas , Extração em Fase Sólida/métodos , Sulfatos/química , Sulfatos/análise , Hidrocarbonetos Iodados
18.
J Agric Food Chem ; 72(30): 16860-16866, 2024 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-39031782

RESUMO

Itaconate is a promising platform chemical with broad applicability, including the synthesis of poly(methyl methacrylate). Most studies on microbial itaconate production entail the use of crop-based feedstock, which imposes constraints due to its limited supply. Brown macroalgae have recently gained attention as next-generation biomass owing to their high biomass productivity and carbohydrate content and amenability to mass production. Therefore, the use of macroalgae for itaconate production warrants exploration. In this study, the direct production of itaconate from brown macroalgae was demonstrated using engineered Vibrio sp. dhg, which has emerged as an efficient platform host for brown macroalgal biorefineries. Specifically, to enhance production, cis-aconitate decarboxylase (Cad) from Aspergillus terreus was heterologously expressed and isocitrate dehydrogenase (icd) was deleted. Notably, the resulting strain, VIC, achieved itaconate titers of 2.5 and 1.5 g/L from a mixture of alginate and mannitol (10 g/L of each) and 40 g/L of raw Saccharina japonica (S. japonica), respectively. Overall, this study highlights the utility of brown macroalgae as feedstock, as well as that of Vibrio sp. dhg as a platform strain for improving itaconate bioproduction.


Assuntos
Engenharia Metabólica , Phaeophyceae , Alga Marinha , Succinatos , Vibrio , Vibrio/metabolismo , Vibrio/genética , Vibrio/crescimento & desenvolvimento , Alga Marinha/metabolismo , Alga Marinha/química , Phaeophyceae/metabolismo , Phaeophyceae/química , Succinatos/metabolismo , Aspergillus/metabolismo , Aspergillus/genética , Aspergillus/enzimologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Biomassa
19.
Mar Pollut Bull ; 206: 116763, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39079478

RESUMO

The biosynthesis of silver nanoparticles, both economically and environmentally advantageous, uses algae extracts. In the current work, we extracted the marine brown alga Hormophysa triquetra (C. Agardh) kützing and used it to make silver nanoparticles (HAgNPs) which are characterized via UV-visible spectrophotometers, Transmission Electron Microscopy (TEM), Zeta potential, and FTIR then used them in the bio adsorption of crude petroleum oil from seawater, comparing them with H. triquetra aqueous extract. UV scan of the phycosynthesized silver nanoparticles achieved the highest absorption at 369 nm. TEM showed that the synthesized HAgNPs occur with smooth, spherical, and semispherical forms with sizes ranging from 12.04 to 20.67 nm, zeta potential illustrated that HAgNPs were charged with -22.1, The H. triquetra aqueous extract's FTIR examination identified several active groups like - OH, -C=C-, NO, -CH, CCl, -C ≡ C-H: CH which are responsible for the bioadsorption of crude petroleum oil. When extracting crude petroleum oil from seawater, HAgNPs worked better than its aqueous extract. The maximum removal % for light n-alkanes (Ln-alk), heavy n-alkanes (Hn-alk), and PAHs were 70.4 %, 71.63 %, and 75.38 % respectively for H. triquetra aqueous extract with adsorption capacity 889, 511, 273 µg/g at salinity 36 % and pH 5, while in case of HAgNPs the results were 75.81 %, 77.15 %, and 80.56 %, respectively with adsorption capacity 957, 550, 292 µg/g at the same salinity and pH.


Assuntos
Nanopartículas Metálicas , Petróleo , Phaeophyceae , Água do Mar , Prata , Poluentes Químicos da Água , Água do Mar/química , Adsorção
20.
Bioresour Technol ; 407: 131073, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38996848

RESUMO

Co-pyrolysis with low-sulfur biomass is expected to improve the yield and quality of bio-fuels, without the usage of calcium-based desulfurizer. Sulfur transformation during microwave fluidized-bed co-pyrolysis between terrestrial and marine biomass (Ascophyllum, AS; Rice straw, RS) was investigated. Sulfur release was promoted during biomass co-pyrolysis, but it was inhibited during pyrolysis between AS and low-sulfur char. Thermal cracking of biomass was promoted during co-pyrolysis between biomass, accelerating the combination of H atoms and -SH radicals. Introduction of low-sulfur bio-char (CA) inhibited the generation of bio-char and the release of sulfur. Released sulfur was captured by -OH/C = C functional groups on bio-char through dehydration reactions/addition reactions, forming mercaptan in bio-char. Furthermore, introduction of microwave and bio-char promoted the cyclization and aromatization reaction, converting mercaptan to thiophene and improving the thermal stability of solid sulfur, and thus increasing in-situ sulfur fixation rate.


Assuntos
Biomassa , Oryza , Phaeophyceae , Enxofre , Enxofre/química , Micro-Ondas , Oryza/química , Phaeophyceae/química , Brotos de Planta/química , Pirólise , Temperatura
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