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1.
J Environ Sci (China) ; 149: 99-112, 2025 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-39181682

RESUMO

With the increasing demand for water in hydroponic systems and agricultural irrigation, viral diseases have seriously affected the yield and quality of crops. By removing plant viruses in water environments, virus transmission can be prevented and agricultural production and ecosystems can be protected. But so far, there have been few reports on the removal of plant viruses in water environments. Herein, in this study, easily recyclable biomass-based carbon nanotubes catalysts were synthesized with varying metal activities to activate peroxymonosulfate (PMS). Among them, the magnetic 0.125Fe@NCNTs-1/PMS system showed the best overall removal performance against pepper mild mottle virus, with a 5.9 log10 removal within 1 min. Notably, the key reactive species in the 0.125Fe@NCNTs-1/PMS system is 1O2, which can maintain good removal effect in real water matrices (river water and tap water). Through RNA fragment analyses and label free analysis, it was found that this system could effectively cleave virus particles, destroy viral proteins and expose their genome. The capsid protein of pepper mild mottle virus was effectively decomposed where serine may be the main attacking sites by 1O2. Long viral RNA fragments (3349 and 1642 nt) were cut into smaller fragments (∼160 nt) and caused their degradation. In summary, this study contributes to controlling the spread of plant viruses in real water environment, which will potentially help protect agricultural production and food safety, and improve the health and sustainability of ecosystems.


Assuntos
Biomassa , Nanotubos de Carbono , Nanotubos de Carbono/química , Vírus de Plantas/fisiologia , Purificação da Água/métodos , Tobamovirus , Peróxidos
2.
PLoS Pathog ; 20(9): e1012473, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39235994

RESUMO

Viroporins are small, hydrophobic viral proteins that modify cellular membranes to form tiny pores for influx of ions and small molecules. Previously, viroporins were identified exclusively in vertebrate viruses. Recent studies have shown that both plant-infecting positive-sense single-stranded (+ss) and negative-sense single-stranded (-ss) RNA viruses also encode functional viroporins. These seminal discoveries not only advance our understanding of the distribution and evolution of viroporins, but also open up a new field of plant virus research.


Assuntos
Doenças das Plantas , Vírus de Plantas , Vírus de Plantas/genética , Vírus de Plantas/fisiologia , Doenças das Plantas/virologia , Proteínas Viroporinas/genética , Proteínas Viroporinas/metabolismo , Plantas/virologia
3.
Planta ; 260(4): 94, 2024 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-39269658

RESUMO

MAIN CONCLUSION: Seed-application of the natural products protects sugar beet and wheat plants against infection with plasmodiophorid-transmitted viruses and thus may represent an efficient, environmentally friendly, easy and cost effective biocontrol strategy. In times of intensive agriculture, resource shortening and climate change, alternative, more sustainable and eco-friendly plant protection strategies are required. Here, we tested the potential of the natural plant substances Glycyrrhiza glabra leaf extract (GE) and the rhamnolipid Rhapynal (Rha) applied to seeds to protect against infection of sugar beet and wheat with soil-borne plant viruses. The soil-borne Polymyxa betae- and Polymyxa graminis-transmitted viruses cause extensive crop losses in agriculture and efficient control strategies are missing. We show that GE and Rha both efficiently protect plants against infection with soil-borne viruses in sugar beet and wheat when applied to seeds. Moreover, the antiviral protection effect is independent of the cultivar used. No protection against Polymyxa sp. was observed after seed treatment with the bio-substances at our analysis time points. However, when we applied the bio-substances directly to soil a significant anti-Polymyxa graminis effect was obtained in roots of barley plants grown in the soil as well as in the treated soil. Despite germination can be affected by high concentrations of the substances, a range of antiviral protection conditions with no effect on germination were identified. Seed-treatment with the bio-substances did not negatively affect plant growth and development in virus-containing soil, but was rather beneficial for plant growth. We conclude that seed treatment with GE and Rha may represent an efficient, ecologically friendly, non-toxic, easy to apply and cost efficient biocontrol measure against soil-borne virus infection in plants.


Assuntos
Beta vulgaris , Glycyrrhiza , Doenças das Plantas , Extratos Vegetais , Sementes , Sementes/virologia , Sementes/efeitos dos fármacos , Doenças das Plantas/virologia , Doenças das Plantas/prevenção & controle , Beta vulgaris/virologia , Beta vulgaris/efeitos dos fármacos , Extratos Vegetais/farmacologia , Triticum/virologia , Triticum/efeitos dos fármacos , Triticum/crescimento & desenvolvimento , Glicolipídeos/farmacologia , Vírus de Plantas/fisiologia , Vírus de Plantas/efeitos dos fármacos , Raízes de Plantas/virologia , Raízes de Plantas/efeitos dos fármacos , Solo/química , Microbiologia do Solo , Hordeum/virologia , Hordeum/efeitos dos fármacos , Plasmodioforídeos/fisiologia , Plasmodioforídeos/efeitos dos fármacos
4.
BMC Genomics ; 25(1): 866, 2024 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-39285355

RESUMO

Transmission of plant viruses by insect vectors is facilitated by unequivocal tri-partite interactions among host plants, viruses, and associated vectors. The advent of next-generation sequencing including whole genome sequencing, RNA/small RNA sequencing, proteomics, and metabolomics aided in elucidating the molecular mechanisms involved in virus transmission by insect vectors and infection in host plants.


Assuntos
Insetos Vetores , Metabolômica , Doenças das Plantas , Vírus de Plantas , Proteômica , Vírus de Plantas/genética , Vírus de Plantas/fisiologia , Insetos Vetores/virologia , Insetos Vetores/genética , Doenças das Plantas/virologia , Doenças das Plantas/genética , Animais , Metabolômica/métodos , Proteômica/métodos , Plantas/virologia , Plantas/metabolismo , Plantas/genética , Genômica/métodos , Interações Hospedeiro-Patógeno/genética , Sequenciamento de Nucleotídeos em Larga Escala
5.
Virology ; 599: 110210, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39213990

RESUMO

Kitaviruses are plant-infecting, non-systemic disease-causing viruses with multipartite single-stranded RNA genomes. Despite their importance, knowledge on kitaviruses is limited in comparison with other plant virus groups, mainly because of the lesser number of identified and characterized kitaviruses and their isolates. In the present study, we explored plant (meta)transcriptome data available in public domain and identified genome sequences of eighteen putative novel blunerviruses in eighteen plant species, including four gymnosperm and four monocot species. Four RNA segments (RNAs 1-4) of eleven identified viruses were recovered, whilst at least two RNA segments were recovered for the remaining viruses. Phylogenetic analysis grouped the identified viruses with known blunerviruses. Based on genome organization, sequence identities of encoded proteins with known blunerviruses and phylogeny, the identified viruses are regarded as new members of the genus Blunervirus. The study paves way for initiating further studies on understanding biological properties, economic importance and geographical distribution of identified blunerviruses.


Assuntos
Mineração de Dados , Variação Genética , Genoma Viral , Filogenia , Doenças das Plantas , Transcriptoma , Doenças das Plantas/virologia , Vírus de Plantas/genética , Vírus de Plantas/classificação , Vírus de Plantas/isolamento & purificação , RNA Viral/genética , Vírus de RNA/genética , Vírus de RNA/classificação , Plantas/virologia
6.
Viruses ; 16(8)2024 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-39205151

RESUMO

In the main cactus pear (Opuntia ficus-indica)-producing region in the State of Mexico, fruit production occupies the largest cultivated area with 15,800 ha, while 900 ha are cultivated for edible young Opuntia pads ("nopalitos") which are consumed as vegetables. Two composite samples consisting of cladodes of plants for fruit production (n = 6) and another of "nopalitos" (n = 6) showing virus-like symptoms were collected. Both sample sets were subjected to high-throughput sequencing (HTS) to identify the viruses and viroids. The HTS results were verified using RT-PCR and Sanger sequencing. Subsequently, 86 samples including cladodes from "nopalitos", plants for fruit production, xoconostles, and some wild Opuntia were analyzed via RT-PCR with specific primers for the viruses and viroids previously detected via HTS. Three viruses were discovered [Opuntia virus 2 (OV2), cactus carlavirus 1 (CCV-1), and Opuntia potexvirus A (OPV-A)], along with a previously reported viroid [Opuntia viroid 1 (OVd-1)]. Additionally, two new viroids were identified, provisionally named the Mexican opuntia viroid (MOVd, genus Pospiviroid) and Opuntia viroid 2 (OVd-2, genus Apscaviroid). A phylogenetic analysis, pairwise identity comparison, and conserved structural elements analysis confirmed the classification of these two viroids as new species within the Pospiviroidae family. This is the first report of a pospiviroid and two apscaviroids infecting cactus pears in the world. Overall, this study enhances our understanding of the virome associated with cactus pears in Mexico.


Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Opuntia , Filogenia , Doenças das Plantas , Viroides , Opuntia/virologia , México , Viroides/genética , Viroides/isolamento & purificação , Viroides/classificação , Doenças das Plantas/virologia , Genoma Viral , Vírus de Plantas/genética , Vírus de Plantas/classificação , Vírus de Plantas/isolamento & purificação , RNA Viral/genética , Frutas/virologia , Carlavirus/genética , Carlavirus/classificação , Carlavirus/isolamento & purificação
7.
Int J Mol Sci ; 25(15)2024 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-39125919

RESUMO

Modern diagnostic techniques based on DNA sequence similarity are currently the gold standard for the detection of existing and emerging pathogens. Whilst individual assays are inexpensive to use, assay development is costly and carries risks of not being sensitive or specific enough to capture an increasingly diverse range of targets. Sequencing can provide the entire nucleic acid content of a sample and may be used to identify all pathogens present in the sample when the depth of coverage is sufficient. Targeted enrichment techniques have been used to increase sequence coverage and improve the sensitivity of detection within virus samples, specifically, to capture sequences for a range of different viruses or increase the number of reads from low-titre virus infections. Vertebrate viruses have been well characterised using in-solution hybridisation capture to target diverse virus families. The use of probes for genotyping and strain identification has been limited in plants, and uncertainty around sensitivity is an impediment to the development of a large-scale virus panel to use within regulatory settings and diagnostic pipelines. This review aims to compare significant studies that have used targeted enrichment of viruses to identify approaches to probe design and potential for use in plant virus detection and characterisation.


Assuntos
Doenças das Plantas , Vírus de Plantas , Vírus de Plantas/isolamento & purificação , Vírus de Plantas/genética , Doenças das Plantas/virologia , Plantas/virologia , Técnicas de Diagnóstico Molecular/métodos
8.
Nat Commun ; 15(1): 7576, 2024 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-39217146

RESUMO

Maize rough dwarf disease (MRDD) threatens maize production globally. The P7-1 effector of the rice black-streaked dwarf virus (RBSDV) targets maize Rab GDP dissociation inhibitor alpha (ZmGDIα) to cause MRDD. However, P7-1 has difficulty recruiting a ZmGDIα variant with an alternative helitron-derived exon 10 (ZmGDIα-hel), resulting in recessive resistance. Here, we demonstrate that P7-1 can recruit another maize protein, gibberellin 2-oxidase 13 (ZmGA2ox7.3), which also exhibits tighter binding affinity for ZmGDIα than ZmGDIα-hel. The oligomerization of ZmGA2ox7.3 is vital for its function in converting bioactive gibberellins into inactive forms. Moreover, the enzymatic activity of ZmGA2ox7.3 oligomers increases when forming hetero-oligomers with P7-1/ZmGDIα, but decreases when ZmGDIα-hel replaces ZmGDIα. Viral infection significantly promotes ZmGA2ox7.3 expression and oligomerization in ZmGDIα-containing susceptible maize, resulting in reduced bioactive GA1/GA4 levels. This causes an auxin/cytokinin imbalance and ultimately manifests as MRDD syndrome. Conversely, in resistant maize, ZmGDIα-hel counters these virus-induced changes, thereby mitigating MRDD severity.


Assuntos
Giberelinas , Doenças das Plantas , Proteínas de Plantas , Zea mays , Zea mays/virologia , Zea mays/metabolismo , Doenças das Plantas/virologia , Giberelinas/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Vírus de Plantas/fisiologia , Resistência à Doença , Regulação da Expressão Gênica de Plantas , Plantas Geneticamente Modificadas , Proteínas Virais/metabolismo , Proteínas Virais/genética
9.
Plant Physiol Biochem ; 215: 109046, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39153391

RESUMO

In the evolutionary arm race between plants and viral pathogens, the plant hormone abscisic acid (ABA) has surfaced as a crucial player. This review accumulates substantial evidence that portrays ABA as a crucial regulatory hub, coordinating the complex network of plant antiviral immunity. It is capable of synchronizing resistance pathways, yet it can also be exploited as a susceptibility factor by viral effectors. ABA fortifies multi-layered defenses on one hand, by activating RNA silencing mechanisms that precisely degrade viral genomes, strengthening plasmodesmal gateways with callose barriers, and priming the transcriptional programs of resistance genes. On the other hand, ABA can augment susceptibility by counteracting other defense hormones, dampening oxidative bursts, and inhibiting antiviral defence proteins. Interestingly, a variety of viruses have independently evolved strategies to manipulate ABA signalling pathways. This fascinating paradigm of hormonal conflicts unveils ABA as an important regulatory handle that determines infection trajectories. Future studies should carefully explore the multifaceted impacts of ABA modulation on plant immunity and susceptibility to diverse pathogens before considering practical applications in viral resistance strategies.


Assuntos
Ácido Abscísico , Doenças das Plantas , Ácido Abscísico/metabolismo , Doenças das Plantas/virologia , Doenças das Plantas/imunologia , Vírus de Plantas/fisiologia , Imunidade Vegetal , Interações Hospedeiro-Patógeno , Reguladores de Crescimento de Plantas/metabolismo , Plantas/virologia , Plantas/metabolismo , Plantas/imunologia , Transdução de Sinais
10.
Viruses ; 16(8)2024 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-39205279

RESUMO

The potato leafhopper (Empoasca fabae, PLH) is a serious pest that feeds on a wide range of agricultural crops and is found throughout the United States but is not known to be a vector for plant-infecting viruses. We probed the diversity of virus sequences in field populations of PLH collected from four Midwestern states: Illinois, Indiana, Iowa, and Minnesota. High-throughput sequencing data from total RNAs extracted from PLH were used to assemble sequences of fifteen positive-stranded RNA viruses, two negative-stranded RNA viruses, and one DNA virus. These sequences included ten previously described plant viruses and eight putative insect-infecting viruses. All but one of the insect-specific viruses were novel and included three solemoviruses, one iflavirus, one phenuivirus, one lispivirus, and one ambidensovirus. Detailed analyses of the novel genome sequences and their evolutionary relationships with related family members were conducted. Our study revealed a diverse group of plant viruses circulating in the PLH population and discovered novel insect viruses, expanding knowledge on the untapped virus diversity in economically important crop pests. Our findings also highlight the importance of monitoring the emergence and circulation of plant-infecting viruses in agriculturally important arthropod pests.


Assuntos
Genoma Viral , Hemípteros , Filogenia , Vírus de Plantas , Animais , Hemípteros/virologia , Vírus de Plantas/genética , Vírus de Plantas/classificação , Vírus de Plantas/isolamento & purificação , Doenças das Plantas/virologia , Doenças das Plantas/parasitologia , Produtos Agrícolas/virologia , Vírus de Insetos/genética , Vírus de Insetos/classificação , Vírus de Insetos/isolamento & purificação , Solanum tuberosum/virologia , Solanum tuberosum/parasitologia , Sequenciamento de Nucleotídeos em Larga Escala , Vírus de DNA/genética , Vírus de DNA/classificação , Vírus de DNA/isolamento & purificação , Vírus de RNA/genética , Vírus de RNA/classificação , Vírus de RNA/isolamento & purificação , Variação Genética , Insetos Vetores/virologia
11.
Viruses ; 16(8)2024 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-39205280

RESUMO

Endogenous viral elements (EVEs) have been reported to exist widely in the genomes of eukaryotic organisms, and they are closely associated with the growth, development, genetics, adaptation, and evolution of their hosts. In this study, two methods-homologous sequence search and genome alignment-were used to explore the endogenous viral sequences in the genomes of Fragaria species. Results revealed abundant endogenous pararetroviruses (EPRVs) in the genomes of Fragaria species, including 786 sequences belonging to five known taxa such as Caulimovirus and other unclassified taxa. Differences were observed in the detected EPRVs between the two methods, with the homologous sequence search having a greater number of EPRVs. On the contrary, genome alignment identified various types and sources of virus-like sequences. Furthermore, through genome alignment, a 267-bp sequence with 95% similarity to the gene encoding the aphid-transmitted protein of Strawberry vein banding virus (Caulimovirus venafragariae) was discovered in the F. chiloensis genome, which was likely a recent insertion. In addition, the statistical analysis of the genome alignment results indicated a remarkably higher abundance of virus-like sequences in the genomes of polyploid strawberries compared with diploid ones. Moreover, the differences in virus-like sequences were observed between the genomes of Fragaria species and those of their close relatives. This study enriched the diversity of viruses that infect strawberries, and laid a theoretical foundation for further research on the origin of endogenous viruses in the strawberry genome, host-virus interactions, adaptation, evolution, and their functions.


Assuntos
Fragaria , Filogenia , Fragaria/virologia , Genoma de Planta , Retrovirus Endógenos/genética , Retrovirus Endógenos/classificação , Vírus de Plantas/genética , Vírus de Plantas/classificação , Vírus de Plantas/isolamento & purificação , Caulimovirus/genética , Caulimovirus/classificação , Genoma Viral
12.
Nat Commun ; 15(1): 6918, 2024 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-39134555

RESUMO

Salivary proteins of insect herbivores can suppress plant defenses, but the roles of many remain elusive. One such protein is glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the saliva of the Recilia dorsalis (RdGAPDH) leafhopper, which is known to transmit rice gall dwarf virus (RGDV). Here we show that RdGAPDH was loaded into exosomes and released from salivary glands into the rice phloem through an exosomal pathway as R. dorsalis fed. In infected salivary glands of R. dorsalis, the virus upregulated the accumulation and subsequent release of exosomal RdGAPDH into the phloem. Once released, RdGAPDH consumed H2O2 in rice plants owing to its -SH groups reacting with H2O2. This reduction in H2O2 of rice plant facilitated R. dorsalis feeding and consequently promoted RGDV transmission. However, overoxidation of RdGAPDH could cause potential irreversible cytotoxicity to rice plants. In response, rice launched emergency defense by utilizing glutathione to S-glutathionylate the oxidization products of RdGAPDH. This process counteracts the potential cellular damage from RdGAPDH overoxidation, helping plant to maintain a normal phenotype. Additionally, salivary GAPDHs from other hemipterans vectors similarly suppressed H2O2 burst in plants. We propose a strategy by which plant viruses exploit insect salivary proteins to modulate plant defenses, thus enabling sustainable insect feeding and facilitating viral transmission.


Assuntos
Hemípteros , Peróxido de Hidrogênio , Oryza , Doenças das Plantas , Saliva , Animais , Hemípteros/virologia , Peróxido de Hidrogênio/metabolismo , Oryza/virologia , Oryza/metabolismo , Doenças das Plantas/virologia , Saliva/metabolismo , Saliva/virologia , Gliceraldeído-3-Fosfato Desidrogenases/metabolismo , Glândulas Salivares/virologia , Glândulas Salivares/metabolismo , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Insetos Vetores/virologia , Floema/virologia , Floema/metabolismo , Reoviridae/fisiologia , Glutationa/metabolismo , Proteínas e Peptídeos Salivares/metabolismo , Vírus de Plantas/fisiologia , Defesa das Plantas contra Herbivoria
13.
Plant J ; 119(5): 2484-2499, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39007841

RESUMO

Pathogen infection induces massive reprogramming of host primary metabolism. Lipid and fatty acid (FA) metabolism is generally disrupted by pathogens and co-opted for their proliferation. Lipid droplets (LDs) that play important roles in regulating cellular lipid metabolism are utilized by a variety of pathogens in mammalian cells. However, the function of LDs during pathogenic infection in plants remains unknown. We show here that infection by rice black streaked dwarf virus (RBSDV) affects the lipid metabolism of maize, which causes elevated accumulation of C18 polyunsaturated fatty acids (PUFAs) leading to viral proliferation and symptom development. The overexpression of one of the two novel LD-associated proteins (LDAPs) of maize (ZmLDAP1 and ZmLDAP2) induces LD clustering. The core capsid protein P8 of RBSDV interacts with ZmLDAP2 and prevents its degradation through the ubiquitin-proteasome system mediated by a UBX domain-containing protein, PUX10. In addition, silencing of ZmLDAP2 downregulates the expression of FA desaturase genes in maize, leading to a decrease in C18 PUFAs levels and suppression of RBSDV accumulation. Our findings reveal that plant virus may recruit LDAP to regulate cellular FA metabolism to promote viral multiplication and infection. These results expand the knowledge of LD functions and viral infection mechanisms in plants.


Assuntos
Ácidos Graxos , Doenças das Plantas , Proteínas de Plantas , Replicação Viral , Zea mays , Zea mays/virologia , Zea mays/metabolismo , Zea mays/genética , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética , Doenças das Plantas/virologia , Ácidos Graxos/metabolismo , Metabolismo dos Lipídeos , Proteínas Associadas a Gotículas Lipídicas/metabolismo , Proteínas Associadas a Gotículas Lipídicas/genética , Gotículas Lipídicas/metabolismo , Gotículas Lipídicas/virologia , Vírus de Plantas/fisiologia , Regulação da Expressão Gênica de Plantas , Reoviridae/fisiologia
14.
New Phytol ; 243(6): 2351-2367, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39030826

RESUMO

Viroids are pathogenic noncoding RNAs that completely rely on their host molecular machinery to accomplish their life cycle. Several interactions between viroids and their host molecular machinery have been identified, including interference with epigenetic mechanisms such as DNA methylation. Despite this, whether viroids influence changes in other epigenetic marks such as histone modifications remained unknown. Epigenetic regulation is particularly important during pathogenesis processes because it might be a key regulator of the dynamism of the defense response. Here we have analyzed the changes taking place in Cucumis sativus (cucumber) facultative and constitutive heterochromatin during hop stunt viroid (HSVd) infection using chromatin immunoprecipitation (ChIP) of the two main heterochromatic marks: H3K9me2 and H3K27me3. We find that HSVd infection is associated with changes in both H3K27me3 and H3K9me2, with a tendency to decrease the levels of repressive epigenetic marks through infection progression. These epigenetic changes are connected to the transcriptional regulation of their expected targets, genes, and transposable elements. Indeed, several genes related to the defense response are targets of both epigenetic marks. Our results highlight another host regulatory mechanism affected by viroid infection, providing further information about the complexity of the multiple layers of interactions between pathogens/viroids and hosts/plants.


Assuntos
Epigênese Genética , Regulação da Expressão Gênica de Plantas , Heterocromatina , Histonas , Doenças das Plantas , Viroides , Heterocromatina/metabolismo , Heterocromatina/genética , Viroides/genética , Viroides/fisiologia , Viroides/patogenicidade , Histonas/metabolismo , Doenças das Plantas/virologia , Doenças das Plantas/genética , Cucumis sativus/virologia , Cucumis sativus/genética , Vírus de Plantas/fisiologia , Vírus de Plantas/patogenicidade , Elementos de DNA Transponíveis/genética , Interações Hospedeiro-Patógeno/genética
15.
J Virol Methods ; 329: 114997, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39059502

RESUMO

The extraction of double stranded (ds) RNA is a common enrichment method for the study, characterization, and detection of RNA viruses. In addition to RNA viruses, viroids, and some DNA viruses, can also be detected from dsRNA enriched extracts which makes it an attractive method for detecting a wide range of viruses when coupled with HTS. Several dsRNA enrichment strategies have been developed. The oldest utilizes the selective binding properties of dsRNA to cellulose. More recent methods are based on the application of anti-dsRNA antibodies and viral proteins with a specific affinity for dsRNA. All three methods have been used together with HTS for plant virus detection and study. To our knowledge, this is the first comparative study of three alternative dsRNA enrichment methods for virus and viroid detection through HTS using virus-infected, and healthy grapevine test plants. Extracts were performed in triplicate using methods based on, the anti-dsRNA antibody mAb rJ2 (Millipore Sigma Canada Ltd, Oakville, ON, Canada), the B2 dsRNA binding protein, and ReliaPrep™ Resin (Promega Corporation, Madison, WI, USA). The results show that the workflows for all three methods are effectively comparable, apart from purification steps related to antibody and binding protein construct. Both the cellulose resin and dsRNA binding protein construct methods provide highly enriched dsRNA extracts suitable for HTS with the B2 method providing a 36× and the ReliaPrep™ Resin a 163× increase in dsRNA enrichment compared to the mAb rJ2 antibody. The overall consistency and cost effectiveness of the ReliaPrep™ cellulose resin-based method and the potentially simpler adaptation to robotics made it the method of choice for future transfer to a semi-automated workflow.


Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Doenças das Plantas , RNA de Cadeia Dupla , RNA Viral , Vitis , RNA de Cadeia Dupla/genética , Vitis/virologia , Sequenciamento de Nucleotídeos em Larga Escala/métodos , RNA Viral/genética , Doenças das Plantas/virologia , Vírus de Plantas/genética , Vírus de Plantas/isolamento & purificação , Vírus de RNA/genética , Vírus de RNA/isolamento & purificação
16.
New Phytol ; 243(4): 1539-1553, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39021237

RESUMO

The interactions among plant viruses, insect vectors, and host plants have been well studied; however, the roles of insect viruses in this system have largely been neglected. We investigated the effects of MpnDV infection on aphid and PVY transmission using bioassays, RNA interference (RNAi), and GC-MS methods and green peach aphid (Myzus persicae (Sulzer)), potato virus Y (PVY), and densovirus (Myzus persicae nicotianae densovirus, MpnDV) as model systems. MpnDV increased the activities of its host, promoting population dispersal and leading to significant proliferation in tobacco plants by significantly enhancing the titer of the sesquiterpene (E)-ß-farnesene (EßF) via up-regulation of expression levels of the MpFPPS1 gene. The proliferation and dispersal of MpnDV-positive individuals were faster than that of MpnDV-negative individuals in PVY-infected tobacco plants, which promoted the transmission of PVY. These results combined showed that an insect virus may facilitate the transmission of a plant virus by enhancing the locomotor activity and population proliferation of insect vectors. These findings provide novel opportunities for controlling insect vectors and plant viruses, which can be used in the development of novel management strategies.


Assuntos
Afídeos , Densovirus , Nicotiana , Doenças das Plantas , Afídeos/virologia , Afídeos/fisiologia , Animais , Nicotiana/virologia , Nicotiana/parasitologia , Doenças das Plantas/virologia , Densovirus/fisiologia , Densovirus/genética , Potyvirus/fisiologia , Potyvirus/patogenicidade , Sesquiterpenos/metabolismo , Vírus de Plantas/fisiologia , Vírus de Plantas/patogenicidade
17.
Virology ; 597: 110160, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-38955083

RESUMO

Plant viruses threaten global food security by infecting commercial crops, highlighting the critical need for efficient virus detection to enable timely preventive measures. Current techniques rely on polymerase chain reaction (PCR) for viral genome amplification and require laboratory conditions. This review explores the applications of CRISPR-Cas assisted diagnostic tools, specifically CRISPR-Cas12a and CRISPR-Cas13a/d systems for plant virus detection and analysis. The CRISPR-Cas12a system can detect viral DNA/RNA amplicons and can be coupled with PCR or isothermal amplification, allowing multiplexed detection in plants with mixed infections. Recent studies have eliminated the need for expensive RNA purification, streamlining the process by providing a visible readout through lateral flow strips. The CRISPR-Cas13a/d system can directly detect viral RNA with minimal preamplification, offering a proportional readout to the viral load. These approaches enable rapid viral diagnostics within 30 min of leaf harvest, making them valuable for onsite field applications. Timely identification of diseases associated with pathogens is crucial for effective treatment; yet developing rapid, specific, sensitive, and cost-effective diagnostic technologies remains challenging. The current gold standard, PCR technology, has drawbacks such as lengthy operational cycles, high costs, and demanding requirements. Here we update the technical advancements of CRISPR-Cas in viral detection, providing insights into future developments, versatile applications, and potential clinical translation. There is a need for approaches enabling field plant viral nucleic acid detection with high sensitivity, specificity, affordability, and portability. Despite challenges, CRISPR-Cas-mediated pathogen diagnostic solutions hold robust capabilities, paving the way for ideal diagnostic tools. Alternative applications in virus research are also explored, acknowledging the technology's limitations and challenges.


Assuntos
Sistemas CRISPR-Cas , Doenças das Plantas , Vírus de Plantas , Vírus de Plantas/genética , Vírus de Plantas/isolamento & purificação , Doenças das Plantas/virologia , Técnicas de Amplificação de Ácido Nucleico/métodos , Técnicas de Diagnóstico Molecular/métodos , RNA Viral/genética , DNA Viral/genética
18.
Annu Rev Plant Biol ; 75(1): 655-677, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-39038248

RESUMO

Viruses, causal agents of devastating diseases in plants, are obligate intracellular pathogens composed of a nucleic acid genome and a limited number of viral proteins. The diversity of plant viruses, their diminutive molecular nature, and their symplastic localization pose challenges to understanding the interplay between these pathogens and their hosts in the currently accepted framework of plant innate immunity. It is clear, nevertheless, that plants can recognize the presence of a virus and activate antiviral immune responses, although our knowledge of the breadth of invasion signals and the underpinning sensing events is far from complete. Below, I discuss some of the demonstrated or hypothesized mechanisms enabling viral recognition in plants, the step preceding the onset of antiviral immunity, as well as the strategies viruses have evolved to evade or suppress their detection.


Assuntos
Doenças das Plantas , Imunidade Vegetal , Vírus de Plantas , Plantas , Vírus de Plantas/fisiologia , Vírus de Plantas/patogenicidade , Vírus de Plantas/imunologia , Vírus de Plantas/genética , Doenças das Plantas/virologia , Doenças das Plantas/imunologia , Plantas/virologia , Plantas/imunologia , Interações Hospedeiro-Patógeno/imunologia , Evasão da Resposta Imune
19.
Arch Insect Biochem Physiol ; 116(3): e22133, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-39054788

RESUMO

Multiple species within the order Hemiptera cause severe agricultural losses on a global scale. Aphids and whiteflies are of particular importance due to their role as vectors for hundreds of plant viruses, many of which enter the insect via the gut. To facilitate the identification of novel targets for disruption of plant virus transmission, we compared the relative abundance and composition of the gut plasma membrane proteomes of adult Bemisia tabaci (Hemiptera: Aleyrodidae) and Myzus persicae (Hemiptera: Aphididae), representing the first study comparing the gut plasma membrane proteomes of two different insect species. Brush border membrane vesicles were prepared from dissected guts, and proteins extracted, identified and quantified from triplicate samples via timsTOF mass spectrometry. A total of 1699 B. tabaci and 1175 M. persicae proteins were identified. Following bioinformatics analysis and manual curation, 151 B. tabaci and 115 M. persicae proteins were predicted to localize to the plasma membrane of the gut microvilli. These proteins were further categorized based on molecular function and biological process according to Gene Ontology terms. The most abundant gut plasma membrane proteins were identified. The ten plasma membrane proteins that differed in abundance between the two insect species were associated with the terms "protein binding" and "viral processes." In addition to providing insight into the gut physiology of hemipteran insects, these gut plasma membrane proteomes provide context for appropriate identification of plant virus receptors based on a combination of bioinformatic prediction and protein localization on the surface of the insect gut.


Assuntos
Afídeos , Trato Gastrointestinal , Proteínas de Insetos , Insetos Vetores , Vírus de Plantas , Animais , Proteínas de Insetos/metabolismo , Insetos Vetores/virologia , Insetos Vetores/metabolismo , Afídeos/virologia , Afídeos/metabolismo , Trato Gastrointestinal/virologia , Trato Gastrointestinal/metabolismo , Proteínas de Membrana/metabolismo , Hemípteros/virologia , Hemípteros/metabolismo , Proteoma , Membrana Celular/metabolismo
20.
Methods Mol Biol ; 2812: 307-315, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39068370

RESUMO

Plants have developed sophisticated defense mechanisms to combat viral infections, prominently utilizing Dicer-like enzymes (DCL) for generating virus-derived small interfering RNAs (vsiRNAs) through RNA interference (RNAi). This intrinsic mechanism effectively impedes virus replication. Exploiting their potential, vsiRNAs have become a major focus area for comprehensive viral investigations in plants, integrating both bioinformatics and experimental strategies. This chapter introduces an up-to-date computational workflow optimized for identifying and comprehensively annotating vsiRNAs with the utilization of small RNA sequencing (sRNA-seq) data collected from virus-infected plants. The workflow detailed in this chapter centers on known plant-targeting viruses, providing step-by-step guidance to enhance vsiRNA analysis, ultimately advancing the comprehension of plant-virus interactions.


Assuntos
Biologia Computacional , RNA Interferente Pequeno , RNA Viral , RNA Interferente Pequeno/genética , RNA Viral/genética , Biologia Computacional/métodos , Vírus de Plantas/genética , Doenças das Plantas/virologia , Doenças das Plantas/genética , Interferência de RNA , Plantas/virologia , Plantas/genética , Análise de Sequência de RNA/métodos , Interações Hospedeiro-Patógeno/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Fluxo de Trabalho
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