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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 324: 124958, 2025 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-39146627

RESUMO

Vanillin is a commonly used synthetic flavoring agent in daily life. However, excessive intake of vanillin may pose risks to human health. Therefore, there is an urgent need for rapid and sensitive detection methods for vanillin. In this study, we developed a fluorescent sensor based on Cd-MOF for the sensitive and selective recognition of vanillin. The presence of vanillin leads to significant fluorescence quenching of Cd-MOF due to competitive absorption and photoinduced electron transfer (PET). The limit of detection was determined to be 39.6 nM, which is the lowest-among the reported fluorescent probes. The sensor was successfully applied for the detection of vanillin in real samples such as powdered milk and milk, with a recovery rate ranging from 96.88 % to 104.83 %. Furthermore, by immobilizing the Cd-MOF probe into a polyvinyl alcohol (PVA) film, we achieved a portable and visual detection composite materials for vanillin.


Assuntos
Benzaldeídos , Estruturas Metalorgânicas , Leite , Espectrometria de Fluorescência , Benzaldeídos/análise , Benzaldeídos/química , Leite/química , Animais , Espectrometria de Fluorescência/métodos , Estruturas Metalorgânicas/química , Pós , Corantes Fluorescentes/química , Limite de Detecção , Cádmio/análise
2.
Bioresour Technol ; 410: 131270, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39147108

RESUMO

The process of preprocessing techniques such as acid and alkali pretreatment in lignocellulosic industry generates substantial solid residues and lignocellulosic pretreatment wastewater (LPW) containing glucose, xylose and toxic byproducts. In this study, furfural and vanillin were selected as model toxic byproducts. Kurthia huakuii as potential strain could tolerate to high concentrations of inhibitors. The results indicated that vanillin exhibited a higher inhibitory effect on K. huakuii (3.95 % inhibition rate at 1 g/L than furfural (0.45 %). However, 0.5 g/L vanillin promoted the bacterial growth (-2.35 % inhibition rate). Interestingly, the combination of furfural and vanillin exhibited antagonistic effects on bacterial growth (Q<0.85). Furfural and vanillin could be bio-transformed into less toxic molecules (furfuryl alcohol, furoic acid, vanillyl alcohol, and vanillic acid) by K. huakuii, and inhibitor degradation rate could be promoted by expression of antioxidant enzymes. This study provides important insights into how bacteria detoxify inhibitors in LPW, potentially enhancing resource utilization.


Assuntos
Benzaldeídos , Biomassa , Lignina , Águas Residuárias , Lignina/metabolismo , Águas Residuárias/química , Benzaldeídos/farmacologia , Furaldeído/farmacologia , Furaldeído/metabolismo , Furaldeído/análogos & derivados , Biodegradação Ambiental , Ácido Vanílico/farmacologia , Ácido Vanílico/metabolismo , Bactérias/metabolismo , Bactérias/efeitos dos fármacos
3.
Int J Mol Sci ; 25(16)2024 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-39201530

RESUMO

The rise of antibiotic tolerance in bacteria harboring genetic elements conferring resistance to antibiotics poses an increasing threat to public health. However, the primary factors responsible for the emergence of antibiotic tolerance and the fundamental molecular mechanisms involved remain poorly comprehended. Here, we demonstrate that the commonly utilized food additive Benzaldehyde (BZH) possesses the capacity to induce a significant level of fluoroquinolone tolerance in vitro among resistant Escherichia coli. Our findings from animal models reveal that the pre-administration of BZH results in an ineffective eradication of bacteria through ciprofloxacin treatment, leading to similar survival rates and bacterial loads as observed in the control group. These results strongly indicate that BZH elicits in vivo tolerance. Mechanistic investigations reveal several key factors: BZH inhibits the formation of bacterial flagella and releases proton motive force (PMF), which aids in expelling antibiotics from within cells to reducing their accumulation inside. In addition, BZH suppresses bacterial respiration and inhibits the production of reactive oxygen species (ROS). Moreover, exogenous pyruvate successfully reverses BZH-induced tolerance and restores the effectiveness of antibiotics, highlighting how crucial the pyruvate cycle is in combating antibiotic tolerance. The present findings elucidate the underlying mechanisms of BZH-induced tolerance and highlight potential hazards associated with the utilization of BZH.


Assuntos
Antibacterianos , Benzaldeídos , Escherichia coli , Flagelos , Benzaldeídos/farmacologia , Flagelos/efeitos dos fármacos , Flagelos/metabolismo , Antibacterianos/farmacologia , Animais , Escherichia coli/efeitos dos fármacos , Escherichia coli/metabolismo , Escherichia coli/genética , Aditivos Alimentares/farmacologia , Aditivos Alimentares/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Ciprofloxacina/farmacologia , Farmacorresistência Bacteriana/efeitos dos fármacos , Farmacorresistência Bacteriana/genética , Camundongos , Testes de Sensibilidade Microbiana
4.
PLoS One ; 19(8): e0308383, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39190744

RESUMO

Microbial volatile organic compounds (VOCs) emitted from fungi are known as their secondary metabolites from environmental sources. However, their physiological roles remain to be unclear. Even though the roles are still unknown, VOCs are deliberately released to convey information to both homologous and non-homologous organisms. We investigated the effects of single VOCs (hexanal, benzaldehyde, heptanal, 2-ethyl-1-hexanol, 3-octanone, 2-undecanone, 3-octanol, 2-Phenylethanol, 2-phenyl-2-propanol, phenylbenzaldehyde, 2-pentadecanone, ß-trans-bergamotene, ß-bisabolene, 2-methyl-5 -(1-methylethyl)pyrazine) on the fungal growth. In parallel, application of the co-culturing system in a growth chamber allowed free gas and VOCs exchange between emitter colonies of Fusarium solani and Aspergillus fumigatus, or between colonies of different growth stages of the same species. Distinct self-inhibition occurred by the emitters of fungal growing colonies against receiver ones on the stage of conidial germination or against the younger colonies at an earlier stage in both fungi. Similarly, the phenomenon of allelopathy appeared to work between growing colonies of F. solani and the germinating conidia or young colonies of A. fumigatus or vice versa. Solid phase microextraction-gas chromatography/mass spectrometry revealed VOCs compounds of each fungi. In F. solani, hexanal and benzaldehyde appeared to be significant inhibitors for colony growth. Benzaldehyde inhibited filamentous growth but not conidial germination. In A. fumigatus, heptanal seemed to be an equivalent effector. The inhibitory effect of benzaldehyde was more distinct on the A. fumigatus conidial germination than its filamentous growth.


Assuntos
Aspergillus fumigatus , Benzaldeídos , Fusarium , Compostos Orgânicos Voláteis , Compostos Orgânicos Voláteis/farmacologia , Compostos Orgânicos Voláteis/metabolismo , Fusarium/efeitos dos fármacos , Fusarium/crescimento & desenvolvimento , Aspergillus fumigatus/crescimento & desenvolvimento , Aspergillus fumigatus/efeitos dos fármacos , Aspergillus fumigatus/metabolismo , Benzaldeídos/farmacologia , Aldeídos/farmacologia , Aldeídos/metabolismo , Esporos Fúngicos/efeitos dos fármacos , Esporos Fúngicos/crescimento & desenvolvimento , Hexanóis/farmacologia , Cetonas/metabolismo
5.
Phytomedicine ; 133: 155941, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39128305

RESUMO

BACKGROUND: Ulcerative colitis (UC), a chronic idiopathic inflammatory bowel disease (IBD), presents with limited current drug treatment options. Consequently, the search for safe and effective drug for UC prevention and treatment is imperative. Our prior studies have demonstrated that the phenolic compound p-Hydroxybenzaldehyde (HD) from Nostoc commune, effectively mitigates intestinal inflammation. However, the mechanisms underlying HD's anti-inflammatory effects remain unclear. PURPOSE: This study delved into the pharmacodynamics of HD and its underlying anti-inflammation mechanisms. METHODS: For in vivo experiments, dextran sodium sulfate (DSS)-induced colitis mouse model was established. In vitro inflammation model was established using lipopolysaccharide (LPS)-induced RAW264.7 and bone marrow-derived macrophages (BMDMs). The protective effect of HD against colitis was determined by monitoring clinical symptoms and histological morphology in mice. The levels of inflammatory factors and oxidative stress markers were subsequently analyzed with enzyme-linked immunosorbent assay (ELISA) and biochemical kits. Furthermore, western blotting (WB), immunofluorescence (IF), luciferase reporter gene, drug affinity reaction target stability (DARTS) assay, molecular docking, and molecular dynamics (MD) simulation were used to determine the potential target and molecular mechanism of HD. RESULTS: Our findings indicate that HD significantly alleviated the clinical symptoms and histological morphology of colitis in mice, and curtailed the production of pro-inflammatory cytokines, including TNF-α, IL-6, IFN-γ, COX-2, and iNOS. Furthermore, HD stimulated the production of SOD, CAT, and GSH-px, enhanced total antioxidant capacity (T-AOC), and reduced MDA levels. Mechanically, HD augmented the expression of Nrf2, HO-1, and NQO-1, while concurrently downregulating the phosphorylation of p65, IκBα, c-Jun, and c-Fos. ML385 and siNrf2 largely attenuated the protective effect of HD in enteritis mice and RAW 264.7 cells, as well as the promotion of HO-1 expression levels. ZnPP-mediated HO-1 knockdown reversed HD-induced inhibition of colonic inflammation. Luciferase reporter assay and IF assay confirmed the transcriptional activation of Nrf2 by HD. DARTS analysis, molecular docking, and MD results showed high binding strength, interaction efficiency and remarkable stability between Nrf2 and HD. CONCLUSION: These outcomes extend our previous research results that HD can combat oxidative stress through the Nrf2/HO-1/NQO-1/NF-κB/AP-1 pathways, effectively alleviating colitis, and propose new targets for HD to protect against intestinal barrier damage.


Assuntos
Benzaldeídos , Sulfato de Dextrana , Fator 2 Relacionado a NF-E2 , NF-kappa B , Estresse Oxidativo , Fator de Transcrição AP-1 , Animais , Fator 2 Relacionado a NF-E2/metabolismo , Camundongos , Benzaldeídos/farmacologia , Estresse Oxidativo/efeitos dos fármacos , NF-kappa B/metabolismo , Células RAW 264.7 , Fator de Transcrição AP-1/metabolismo , Masculino , Anti-Inflamatórios/farmacologia , Camundongos Endogâmicos C57BL , NAD(P)H Desidrogenase (Quinona)/metabolismo , Colite/tratamento farmacológico , Colite/induzido quimicamente , Modelos Animais de Doenças , Colite Ulcerativa/tratamento farmacológico , Colite Ulcerativa/induzido quimicamente , Transdução de Sinais/efeitos dos fármacos , Heme Oxigenase-1/metabolismo , Lipopolissacarídeos , Heme Oxigenase (Desciclizante)/metabolismo , Proteínas de Membrana/metabolismo
6.
Biomater Adv ; 164: 213981, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39096587

RESUMO

Generally, oligolysine has poor antibacterial effect and almost no antibacterial activity. Herein, low cost and easily available oligolysines were chosen to prepare injectable antibacterial hydrogel (PVAL-gel) for wound healing. The hydrogel network was formed by cross-linking vanillin acrylate-N, N-dimethylacrylamide copolymer P(VA-co-DMA), oligolysine and adipate dihydrazide through Schiff base bond. The obtained hydrogel PVAL-gel exhibited not only excellent self-healing capability and injectability, but also the efficient contact antibacterial ability and good inhibitory effects on E.coli and S.aureus. In vitro, 99.9 % of pathogenic bacteria was killed within 160 min. Furthermore, the injectable PVAL-gel could rapidly eradicate bacteria in infected wounds and notably enhance the healing of full-thickness skin wounds. Therefore, PVAL-gel is expected to be used as a high-end dressing for the treatment of infected skin wounds, which can promote wound healing.


Assuntos
Antibacterianos , Escherichia coli , Hidrogéis , Staphylococcus aureus , Cicatrização , Cicatrização/efeitos dos fármacos , Hidrogéis/química , Hidrogéis/farmacologia , Hidrogéis/administração & dosagem , Antibacterianos/farmacologia , Antibacterianos/química , Antibacterianos/administração & dosagem , Escherichia coli/efeitos dos fármacos , Animais , Staphylococcus aureus/efeitos dos fármacos , Benzaldeídos/química , Benzaldeídos/farmacologia , Benzaldeídos/administração & dosagem , Testes de Sensibilidade Microbiana , Injeções , Adipatos/química , Adipatos/farmacologia , Camundongos , Acrilamidas/química , Acrilamidas/farmacologia , Polilisina/química , Polilisina/farmacologia
7.
Bioresour Technol ; 408: 131190, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39094966

RESUMO

Production of the high industrial value cis,cis-muconic acid (ccMA) from renewable biomasses is of main interest especially when biological (green) processes are used. We recently generated a E. coli strain expressing five recombinant enzymes to convert vanillin (VA, from lignin) into ccMA. Here, we optimized a growing cell approach in bioreactor for the ccMA production. The medium composition, fermentation conditions, and VA addition were tuned: pulse-feeding VA at 1 mmol/h allowed to reach 5.2 g/L of ccMA in 48 h (0.86 g ccMA/g VA), with a productivity 4-fold higher compared to the resting cells approach, thus resulting in significantly lower E-factor and Process Mass Intensity green metric parameters. The recovered ccMA has been used as building block to produce a fully bioderived polymer with rubber-like properties. The sustainable optimized bioprocess can be considered an integrated approach to develop a platform for bio-based polymers production from renewable feedstocks.


Assuntos
Reatores Biológicos , Escherichia coli , Ácido Sórbico , Ácido Sórbico/análogos & derivados , Ácido Sórbico/metabolismo , Escherichia coli/metabolismo , Fermentação , Benzaldeídos/metabolismo , Polímeros/química , Biotecnologia/métodos , Biomassa
8.
Nano Lett ; 24(33): 10139-10147, 2024 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-39109658

RESUMO

Surface-enhanced Raman scattering (SERS) offers a promising, cost-effective alternative for the rapid, sensitive, and quantitative analysis of potential biomarkers in exhaled gases, which is crucial for early disease diagnosis. However, a major challenge in SERS is the effective detection of gaseous analytes, primarily due to difficulties in enriching and capturing them within the substrate's "hotspot" regions. This study introduces an advanced gas sensor combining mesoporous gold (MesoAu) and metal-organic frameworks (MOFs), exhibiting high sensitivity and rapid detection capabilities. The MesoAu provides abundant active sites and interconnected mesopores, facilitating the diffusion of analytes for detection. A ZIF-8 shell enveloping MesoAu further enriches target molecules, significantly enhancing sensitivity. A proof-of-concept experiment demonstrated a detection limit of 0.32 ppb for gaseous benzaldehyde, indicating promising prospects for the rapid diagnosis of early stage lung cancer. This research also pioneers a novel approach for constructing hierarchical plasmonic nanostructures with immense potential in gas sensing.


Assuntos
Testes Respiratórios , Gases , Ouro , Estruturas Metalorgânicas , Análise Espectral Raman , Estruturas Metalorgânicas/química , Testes Respiratórios/métodos , Ouro/química , Gases/análise , Gases/química , Humanos , Análise Espectral Raman/métodos , Porosidade , Nanoestruturas/química , Benzaldeídos/química , Limite de Detecção , Nanopartículas Metálicas/química
9.
J Chromatogr A ; 1732: 465210, 2024 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-39098100

RESUMO

Various crossover phenomena are immanent to supercritical fluids due to multidirectional temperature effects in highly compressible supercritical fluid media. Solubility crossover, i.e. controversial effect of temperature on solubility at different pressures, is probably the most well-known among them. A curious discrepancy in upper crossover pressure values between solubility in supercritical carbon dioxide and retention in supercritical fluid chromatography with pure CO2 as an eluent was unexpectedly observed for several non-polar compounds on different stationary phases. In some cases, retention crossover was found to happen at pressures almost twice as high as pressures for solubility crossover for the same compound. Retention data for three solutes with known solubility crossovers: anthracene, benzoic acid and vanillin, were collected at different pressures and temperatures for several stationary phases. The existence of upper retention crossovers, i.e. such pressure values beyond which temperature increase starts decreasing retention, were registered for all solute-sorbent combinations. Using known thermodynamic models of temperature effect on retention in supercritical fluid chromatography and on solubility in supercritical carbon dioxide, possible reasoning for the observed discrepancies is discussed. Major contribution of the balance between adsorption and partition retention mechanisms in defining retention crossover values is hypothesized.


Assuntos
Dióxido de Carbono , Cromatografia com Fluido Supercrítico , Pressão , Solubilidade , Temperatura , Cromatografia com Fluido Supercrítico/métodos , Dióxido de Carbono/química , Antracenos/química , Ácido Benzoico/química , Termodinâmica , Benzaldeídos/química , Adsorção
10.
Int J Immunopathol Pharmacol ; 38: 3946320241271724, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39116410

RESUMO

This study aimed to investigate whether the beneficial effects of PCA on chondrocyte senescence are mediated through the regulation of mitophagy. Chondrocyte senescence plays a significant role in the development and progression of knee osteoarthritis (OA). The compound protocatechuic aldehyde (PCA), which is abundant in the roots of Salvia miltiorrhiza, has been reported to have antioxidant properties and the ability to protect against cellular senescence. To achieve this goal, a destabilization of the medial meniscus (DMM)-induced mouse OA model and a lipopolysaccharide (LPS)-induced chondrocyte senescence model were used, in combination with PINK1 gene knockdown or overexpression. After treatment with PCA, cellular senescence was assessed using Senescence-Associated ß-Galactosidase (SA-ß-Gal) staining, DNA damage was evaluated using Hosphorylation of the Ser-139 (γH2AX) staining, reactive oxygen species (ROS) levels were measured using Dichlorodihydrofluorescein diacetate (DCFH-DA) staining, mitochondrial membrane potential was determined using a 5,5',6,6'-TETRACHLORO-1,1',3,3'-*. TETRAETHYBENZIMIDA (JC-1) kit, and mitochondrial autophagy was examined using Mitophagy staining. Western blot analysis was also performed to detect changes in senescence-related proteins, PINK1/Parkin pathway proteins, and mitophagy-related proteins. Our results demonstrated that PCA effectively reduced chondrocyte senescence, increased the mitochondrial membrane potential, facilitated mitochondrial autophagy, and upregulated the PINK1/Parkin pathway. Furthermore, silencing PINK1 weakened the protective effects of PCA, whereas PINK1 overexpression enhanced the effects of PCA on LPS-induced chondrocytes. PCA attenuates chondrocyte senescence by regulating PINK1/Parkin-mediated mitochondrial autophagy, ultimately reducing cartilage degeneration.


Assuntos
Benzaldeídos , Catecóis , Senescência Celular , Condrócitos , Mitofagia , Proteínas Quinases , Ubiquitina-Proteína Ligases , Condrócitos/efeitos dos fármacos , Condrócitos/metabolismo , Condrócitos/patologia , Animais , Senescência Celular/efeitos dos fármacos , Ubiquitina-Proteína Ligases/metabolismo , Ubiquitina-Proteína Ligases/genética , Mitofagia/efeitos dos fármacos , Proteínas Quinases/metabolismo , Camundongos , Catecóis/farmacologia , Benzaldeídos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Mitocôndrias/patologia , Masculino , Camundongos Endogâmicos C57BL , Autofagia/efeitos dos fármacos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Osteoartrite do Joelho/patologia , Osteoartrite do Joelho/metabolismo , Osteoartrite do Joelho/tratamento farmacológico
11.
Int J Biol Macromol ; 278(Pt 3): 134933, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39173795

RESUMO

Thermosetting resins are widely used in high-tech applications for excellent mechanical robustness and chemical resistance. With increasing attention to the environmental and usage safety issues, it is necessary to develop bio-derived, recyclable, tough, and fire-retardant thermosetting resins. Herein, a high-performance, vanillin-based vitrimer (CIP1.0) was prepared. The CIP1.0 with 1.0 wt% phosphorus passes vertical burning (UL-94) V-0 rating with a limiting oxygen index (LOI) of 27.2%. The phosphorus-containing and Schiff base groups act synergistically in gas and condensed phases during combustion, endowing CIP1.0 with outstanding fire retardancy. The CIP1.0 shows excellent toughness with high elongation at break of 45.0% due to the π-π stacking of numerous rigid aromatic groups and appropriate cross-linking density. The highly symmetrical structure and low polarizability of CIP1.0 result in a low dielectric constant. The CIP1.0 exhibits superior antimicrobial properties. The CIP1.0 can be reprocessed by hot-pressing at 140 °C for 10 min. The non-destructive, closed-loop recycling of carbon fibers in the carbon fiber-reinforced CIP1.0 composite can be achieved under mild conditions due to the degradable Schiff base groups of CIP1.0. In this work, a bio-derived, tough, fire-retardant, low dielectric, and antimicrobial vitrimer is prepared to provide a rational strategy for the design of advanced environmentally friendly thermosetting resins.


Assuntos
Antibacterianos , Benzaldeídos , Retardadores de Chama , Bases de Schiff , Bases de Schiff/química , Antibacterianos/química , Antibacterianos/farmacologia , Benzaldeídos/química , Benzaldeídos/farmacologia , Reciclagem
12.
Int Immunopharmacol ; 141: 113016, 2024 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-39182269

RESUMO

The accumulating evidence has made it clear that iron overload is a crucial mechanism in bone loss. Protocatechualdehyde (PCA) has also been used to prevent osteoporosis in recent years. Whether PCA can reverse the harmful effects of iron overload on bone mass in aged rats is still unknown. Therefore, this study aimed to assess the role of PCA in iron overload-induced bone loss in senile rats. In the aged rat model, we observed that iron overload affects bone metabolism and bone remodeling, manifested by bone loss and decreased bone mineral density. The administration of PCA effectively mitigated the detrimental effects caused by iron overload, and concomitant reduction in MDA serum levels and elevation of SOD were noted. In addition, PCA-treated rats were observed to have significantly increased bone mass and elevated expression of SIRT3,BMP2,SOD2 and reduced expression of TNF-α in bone tissue. We also observed that PCA was able to reduce oxidative stress and inflammation and restore the imbalance in bone metabolism. When MC3T3-E1 and RAW264.7 cells induced osteoblast and osteoclasts differentiation, PCA intervention could significantly recover the restriction of osteogenic differentiation and up-regulation of osteoclast differentiation treated by iron overload. Further, by detecting changes in ROS, SOD, MDA, expression of SIRT3 and mitochondrial membrane potentials, we confirm that the damage caused to cells by iron overload is associated with decreased SIRT3 activity, and that 3-TYP have similar effects on oxidative stress caused by FAC. In conclusion, PCA can resist iron overload-induced bone damage by improving SIRT3 activity, anti-inflammatory and anti-oxidative stress.


Assuntos
Sobrecarga de Ferro , Estresse Oxidativo , Animais , Estresse Oxidativo/efeitos dos fármacos , Sobrecarga de Ferro/metabolismo , Camundongos , Ratos , Masculino , Células RAW 264.7 , Ratos Sprague-Dawley , Osteoclastos/efeitos dos fármacos , Osteoclastos/metabolismo , Sirtuína 3/metabolismo , Sirtuína 3/genética , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Osteoporose/metabolismo , Osteoporose/etiologia , Osteoporose/tratamento farmacológico , Benzaldeídos/farmacologia , Benzaldeídos/uso terapêutico , Inflamação , Osteogênese/efeitos dos fármacos , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Densidade Óssea/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Envelhecimento , Modelos Animais de Doenças , Superóxido Dismutase/metabolismo , Sirtuínas
13.
Lab Chip ; 24(18): 4306-4320, 2024 09 10.
Artigo em Inglês | MEDLINE | ID: mdl-39207360

RESUMO

Saliva has emerged as a primary biofluid for non-invasive disease diagnostics. Saliva collection involves using kits where individuals stimulate saliva production via a chewing device like a straw, then deposit the saliva into a designated collection tube. This process may pose discomfort to patients due to the necessity of producing large volumes of saliva and transferring it to the collection vessel. This work has developed a saliva collection and analysis device where the patient operates it like a lollipop, stimulating saliva production. The lollipop-mimic device contains yarn-based microfluidic channels that sample saliva and transfer it to the sensing zone embedded in the stem of the device. We have embedded electrochemical sensors in the lollipop platform to measure vanillin levels in saliva. Vanillin is the most common food flavoring additive and is added to most desserts such as ice cream, cakes, and cookies. Overconsumption of vanillin can cause side effects such as muscle weakness, and damage to the liver, kidneys, stomach, and lungs. We detected vanillin using direct oxidation at a laser-induced graphene (LIG) electrode. We showed a dynamic range of 2.5 µM to 30 µM, covering the physiologically relevant concentration of vanillin in saliva. The lab-on-a-lollipop platform requires only 200 µL of saliva and less than 2 minutes to fill the channels and complete the measurement. This work introduces the first sensor-embedded lollipop-mimic saliva collection and measurement system.


Assuntos
Benzaldeídos , Técnicas Eletroquímicas , Saliva , Saliva/química , Benzaldeídos/química , Benzaldeídos/análise , Humanos , Técnicas Eletroquímicas/instrumentação , Dispositivos Lab-On-A-Chip , Desenho de Equipamento
14.
Bioresour Technol ; 411: 131189, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39127360

RESUMO

Vanillin is an important flavouring agent applied in food, spices, pharmaceutical industries and other fields. Microbial biosynthesis of vanillin is considered a sustainable and economically feasible alternative to traditional chemical synthesis. In this study, Escherichia coli K12 MG1655 was used for the de novo synthesis of VAN by screening highly active carboxylic acid reductases and catechol O-methyltransferases, optimising the protocatechuic acid pathway, and regulating competitive metabolic pathways. Additionally, major alcohol by-products were identified and decreased by deleting three endogenous aldo-keto reductases and three alcohol dehydrogenases. Finally, a highest VAN titer was achieved to 481.2 mg/L in a 5 L fermenter from glucose. This work provides a valuable example of pathway engineering and screens several enzyme variants for the first time in E. coli.


Assuntos
Benzaldeídos , Escherichia coli K12 , Engenharia Metabólica , Benzaldeídos/metabolismo , Engenharia Metabólica/métodos , Escherichia coli K12/metabolismo , Escherichia coli K12/genética , Fermentação
15.
J Biotechnol ; 394: 112-124, 2024 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-39197754

RESUMO

Vanillin is an inhibitor of lignocellulose hydrolysate, which can reduce the ability of Saccharomyces cerevisiae to utilize lignocellulose, which is an important factor limiting the development of the ethanol fermentation industry. In this study, mutants of vanillin-tolerant yeast named H6, H7, X3, and X8 were bred by heavy ion irradiation (HIR) combined with adaptive laboratory evolution (ALE). Phenotypic tests revealed that the mutants outperformed the original strain WT in tolerance, growth rate, genetic stability and fermentation ability. At 1.6 g/L vanillin concentration, the average OD600 value obtained for mutant strains was 0.95 and thus about 3.4-fold higher than for the wild-type. When the concentration of vanillin was 2.0 g/L, the glucose utilization rate of the mutant was 86.3 % within 96 h, while that of the original strain was only 70.0 %. At this concentration of vanillin, the mitochondrial membrane potential of the mutant strain recovered faster than that of the original strain, and the ROS scavenging ability was stronger. We analyzed the whole transcriptome sequencing map and the whole genome resequencing of the mutant, and found that DEGs such as FLO9, GRC3, PSP2 and SWF1, which have large differential expression multiples and obvious mutation characteristics, play an important role in cell flocculation, rDNA transcription, inhibition of DNA polymerase mutation and protein palmitoylation. These functions can help cells resist vanillin stress. The results show that combining HIR with ALE is an effective mutagenesis strategy. This approach can efficiently obtain Saccharomyces cerevisiae mutants with improved vanillin tolerance, and provide reference for obtaining robust yeast strains with lignocellulose inhibitor tolerance.


Assuntos
Benzaldeídos , Saccharomyces cerevisiae , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/metabolismo , Benzaldeídos/farmacologia , Benzaldeídos/metabolismo , Fermentação , Íons Pesados , Evolução Molecular Direcionada/métodos , Mutação , Lignina/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo , Etanol/metabolismo , Etanol/farmacologia
16.
J Agric Food Chem ; 72(36): 20064-20076, 2024 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-39196852

RESUMO

Vanillin (VAN) is a common flavoring agent that can cause liver damage when ingested in large amounts. Nevertheless, the precise processes responsible for its toxicity remain obscure. The present research aimed to examine the metabolic activation of VAN and establish a potential correlation between its reactive metabolites and its cytotoxicity. In rat liver microsomes incubated with VAN, reduced glutathione/N-acetylcysteine (GSH/NAC), and nicotinamide adenine dinucleotide phosphate (NADPH), two conjugates formed from GSH and one conjugate derived from NAC were identified. We also discovered one GSH conjugate in both the bile obtained from rats and the rat primary hepatocytes that were subjected to VAN exposure. Additionally, the NAC conjugate exerted in the urine of VAN-treated rats was observed. These results indicate that a quinone intermediate was produced from VAN both in vitro and in vivo. Next, we identified CYP3A as the main enzyme that initiated the bioactive pathway of VAN. After the activity of CYP3A was selectively inhibited by ketoconazole (KTZ), the generation of the GSH conjugate declined in hepatocytes exposed to VAN. Furthermore, the vulnerability to VAN-induced toxicity was alleviated by KTZ in hepatocytes. Thus, we propose that the cytotoxicity of VAN may derive from metabolic activation triggered by CYP3A.


Assuntos
Ativação Metabólica , Benzaldeídos , Citocromo P-450 CYP3A , Hepatócitos , Microssomos Hepáticos , Ratos Sprague-Dawley , Animais , Benzaldeídos/metabolismo , Benzaldeídos/farmacologia , Hepatócitos/metabolismo , Hepatócitos/efeitos dos fármacos , Ratos , Masculino , Citocromo P-450 CYP3A/metabolismo , Citocromo P-450 CYP3A/genética , Microssomos Hepáticos/metabolismo , Microssomos Hepáticos/efeitos dos fármacos , Glutationa/metabolismo , Aromatizantes/metabolismo , Aromatizantes/química , Aromatizantes/toxicidade
17.
Vet Parasitol ; 331: 110285, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39126894

RESUMO

Economic importance of ticks for both humans and animals is significant primarily because of their involvement in disease transmission. The continuous use of synthetic chemical acaricides on animals and/or in the environment has resulted in the emergence of resistant tick populations. Consequently, there is a compelling need to explore newer natural alternatives that can address their adverse effects effectively. Since, there are no earlier reported studies on the acaricidal activity of eugenol and benzaldehyde against Rhipicephalus annulatus, the present study compared the in vitro adulticidal and larvicidal activities of these phytochemicals against R. annulatus. Concentration-dependent mortality and inhibition of fecundity were observed in the adult female ticks treated with both eugenol and benzaldehyde. The LC50 and LC90 values of eugenol against R. annulatus were 162.33 and 467.74 mg/mL for adult female, while it was 1.26 and 1.73 mg/mL for larvae respectively. The LC50 and LC90 values of benzaldehyde against R. annulatus were 178 and 395 mg/mL for adult female and 0.69 and 4.32 mg/mL for larvae respectively.


Assuntos
Acaricidas , Benzaldeídos , Eugenol , Larva , Rhipicephalus , Animais , Eugenol/farmacologia , Acaricidas/farmacologia , Feminino , Benzaldeídos/farmacologia , Rhipicephalus/efeitos dos fármacos , Larva/efeitos dos fármacos , Dose Letal Mediana
18.
Int J Biol Macromol ; 278(Pt 1): 134558, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39128753

RESUMO

Polylactic acid (PLA) is widely known for its biocompatibility, biodegradability, and high transparency. However, it still has varied limitations such as flammability, UV sensitivity, and poor oxygen barrier properties. To address these issues, a bio-based compound, hexasubstituted cyclotriphosphazene (HVP), was synthesized by using vanillin and hexachlorocyclotriphosphazene to enhance the overall performance of PLA. The resulting PLA/HVP composites demonstrated improved mechanical strength and UV resistance. Specifically, PLA/3HVP, with a 3 wt% HVP loading, achieved a UL-94 V-0 rating and a high limiting oxygen index of 26.5 %. Cone calorimeter tests revealed that PLA/3HVP possessed a significantly longer ignition time and a lower peak heat release rate compared to pure PLA. These burning testing results indicated the enhanced fire resistance. Additionally, the oxygen transmission rate of PLA/3HVP was reduced by 81.1 % compared to pure PLA. When used as food packaging, the weight loss of mangoes covered with PLA/3HVP film was 2.2 % after 7 days, compared to 2.5 % with pure PLA film, highlighting its potential for food preservation applications.


Assuntos
Benzaldeídos , Retardadores de Chama , Embalagem de Alimentos , Oxigênio , Poliésteres , Raios Ultravioleta , Poliésteres/química , Embalagem de Alimentos/métodos , Benzaldeídos/química , Oxigênio/química
19.
J Agric Food Chem ; 72(34): 19120-19130, 2024 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-39141612

RESUMO

The demand for natural Vanilla has increased rapidly, creating the need for more potential sources of high-quality Vanilla essence. Understanding the geographical influences on the aroma profile of Vanilla is essential. This study demonstrates the first comparative analysis of odorant compositions in the three most important Vanilla varieties: Vanilla planifolia, Vanilla pompona, and Vanilla tahitensis from different origins. Following the screening for odor-active molecules through gas chromatography-olfactometry and aroma extract dilution analysis (GC-O and AEDA), selected compounds were quantified using stable isotope dilution assays (SIDA) and their dose over threshold values (DoTs) were calculated. Vanillin was confirmed as the most important odor-active compound due to its highest DoT value, especially in the V. planifolia sample. Meanwhile, 4-methoxybenzyl alcohol and 4-methoxybenzaldehyde showed higher DoT factors than vanillin in V. pompona and partially in V. tahitensis samples. This indicates their role as discriminative odorants for these varieties. The heightened DoT values of 3-hydroxy-4,5-dimethyl-2(5H)-furanone in Uganda Vanilla samples unveil geographical influences on the odorant profile within V. planifolia species. Additionally, 2-methyl-3-(methyldithio)furan was identified for the first time in Vanilla samples with diverse DoT values from different species and origins.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas , Odorantes , Vanilla , Compostos Orgânicos Voláteis , Vanilla/química , Vanilla/classificação , Odorantes/análise , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/análise , Olfatometria , Benzaldeídos/análise , Sementes/química , Aromatizantes/química
20.
Anal Methods ; 16(33): 5676-5683, 2024 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-39118596

RESUMO

In this study, we describe a rapid and high-throughput smartphone-based digital colorimetric method for determining urea in milk. A compact and cost-effective 3D-printed image box microplate-based system was designed to measure multiple samples simultaneously, using minimal sample and reagent volumes. The apparatus was applied for the quantification of urea in milk based on its reaction with p-dimethylaminobenzaldehyde (DMAB). The predictive performance of calibration was evaluated using RGB and different colour models (CMYK, HSV, and CIELAB), with the average blue (B) values of the RGB selected as the analytical signal for urea quantification. Under optimized conditions, a urea concentration linear range from 50 to 400 mg L-1 was observed, with a limit of detection (LOD) of 15 mg L-1. The values found with the smartphone-based DIC procedure are in good agreement with spectrophotometric (spectrophotometer and microplate treader) and reference method (mid-infrared spectroscopy) values. This proposed approach offers an accessible and efficient solution for digital image colorimetry, with potential applications for various target analytes in milk and other fields requiring high-throughput colorimetric analysis.


Assuntos
Colorimetria , Leite , Impressão Tridimensional , Smartphone , Ureia , Leite/química , Colorimetria/métodos , Colorimetria/instrumentação , Animais , Ureia/análise , Ureia/química , Limite de Detecção , Benzaldeídos/química , Benzaldeídos/análise
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