Assessment of poly(diallyl dimethyl ammonium chloride) and lime for surface water treatment (pond, river, and canal water): seasonal variations and correlation analyses.

The present study deals with the assessment of different physicochemical parameters (pH, electrical conductivity (E.C.), turbidity, total dissolved solids (TDS), and dissolved oxygen) in different surface water such as pond, river, and canal water in four different seasons, viz. March, June, September, and December 2023. The research endeavors to assess the impact of a cationic polyelectrolyte, specifically poly(diallyl dimethyl ammonium chloride) (PDADMAC), utilized as a coagulation aid in conjunction with lime for water treatment. Employing a conventional jar test apparatus, turbidity removal from diverse water samples is examined. Furthermore, the samples undergo characterization utilizing X-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques. The study also conducts correlation analyses on various parameters such as electrical conductivity (EC), pH, total dissolved solids (TDS), turbidity of raw water, polyelectrolyte dosage, and percentage of turbidity removal across different water sources. Utilizing the Statistical Package for Social Science (SPSS) software, these analyses aim to establish robust relationships among initial turbidity, temperature, percentage of turbidity removal, dosage of coagulant aid, electrical conductivity, and total dissolved solids (TDS) in pond water, river water, and canal water. A strong positive correlation could be found between the percentage of turbidity removal and the value of initial turbidity of all surface water. However, a negative correlation could be observed between the polyelectrolyte dosage and raw water's turbidity. By elucidating these correlations, the study contributes to a deeper understanding of the effectiveness of PDADMAC and lime in water treatment processes across diverse environmental conditions. This research enhances our comprehension of surface water treatment methodologies and provides valuable insights for optimizing water treatment strategies to address the challenges posed by varying water sources and seasonal fluctuations.

Present and future distribution of the European pond turtle versus seven exotic freshwater turtles, with a focus on Eastern Europe.

Freshwater turtles are often used as terrarium pets, especially juveniles of exotic species. At the adult stage they are often released by their owners into the wild despite their high invasion potential. In Europe these thermophilic potentially invasive alien species occupy the habitats of the native European pond turtle Emys orbicularis (Linnaeus, 1758), with new records from the wild being made specifically in Eastern Europe (Latvia and Ukraine) during recent decades. Assessing the potential of alien freshwater turtles to establish in new territories is of great concern for preventing invasion risks while preserving native biodiversity in the present context of climate change. We explored this issue by identifying the present and future (by 2050) suitable habitats of the European pond turtle and several potentially invasive alien species of freshwater turtle already settled in Europe, using a geographic information system (GIS) modelling approach based on datasets from CliMond for climate, Near-global environmental information (NGEI) for freshwater ecosystems (EarthEnv) and Maxent modelling using open-access databases, data from the literature and original field data. Modelling was performed for seven species of alien freshwater turtles occurring from the extreme northern to southern borders of the European range of E. orbicularis: the pond slider Trachemys scripta (Thunberg and Schoepff, 1792), the river cooter Pseudemys concinna (Le Conte, 1830), the Florida red-bellied cooter Pseudemys nelsoni (Carr, 1938), the false map turtle Graptemys pseudogeographica (Gray, 1831), the Chinese softshell turtle Pelodiscus sinensis (Wiegmann, 1835), the Caspian turtle Mauremys caspica (Gmelin, 1774) and the Balkan terrapin Mauremys rivulata (Valenciennes, 1833). In Ukraine, the most Eastern limit of E. orbicularis distribution, were previously reported northern American originated T. scripta, M. rivulata, M. caspica, whereas in Latvia, Emys' most northern limit, were additionally reported P. concinna, P. nelsoni, G. pseudogeographica and Asia originated P. sinensis. The resulting Species Distribution Models (SDM) were of excellent performance (AUC > 0.8). Of these alien species, the most potentially successful in terms of range expansion throughout Europe were T. scripta (34.3% of potential range expansion), G. pseudogeographica (24.1%), and M. caspica (8.9%) and M. rivulata (4.3%) mainly in Eastern Europe, especially in the south of Ukraine (Odesa, Kherson, Zaporizhzhia regions, and Crimean Peninsula). Correlation between the built SDMs for the native E. orbicularis and the invasive alien T. scripta was reliably high, confirming the highly likely competition between these two species in places they cooccur. Moreover, a Multiple Regression Analysis revealed that by 2050, in most of Europe (from the western countries to Ukraine), the territory overlap between E. orbicularis and potentially invasive alien species of freshwater turtles will increase by 1.2 times, confirming higher competition in the future. Importantly, by 2050, Eastern Europe and Ukraine are predicted to be the areas with most suitable habitats for the European pond turtle yet with most limited overlap with the invasive alien species. We conclude that Eastern Europe and Ukraine are the most relevant priority conservation areas for the European pond turtle where it is now necessary to take protective measures to ensure safe habitat for this native species on the long-term.

Losses and destabilization of soil organic carbon stocks in coastal wetlands converted into aquaculture ponds.

Coastal-wetlands play a crucial role as carbon (C) reservoirs on Earth due to their C pool composition and functional sink, making them significant for mitigating global climate change. However, due to the development and utilization of wetland resources, many wetlands have been transformed into other land-use types. The current study focuses on the alterations in soil organic-C (SOC) in coastal-wetlands following reclamation into aquaculture ponds. We conducted sampling at 11 different coastal-wetlands along the tropical to temperate regions of the China coast. Each site included two community types, one with solely native species (Suaeda salsa, Phragmites australis and Mangroves) and the other with an adjacent reclaimed aquaculture pond. Across these 11 locations we compared SOC stock, active OC fractions, and soil physicochemical properties between coastal wetlands and aquaculture ponds. We observed that different soil uses, sampling sites, and their interaction had significant effects on SOC and its stock (p < .05). Reclamation significantly declined SOC concentration at depths of 0-15 cm and 15-30 cm by 35.5% and 30.3%, respectively, and also decreased SOC stock at 0-15 cm and 15-30 cm depths by 29.1% and 37.9%, respectively. Similar trends were evident for SOC stock, labile organic-C, dissolved organic-C and microbial biomass organic-C concentrations (p < .05), indicating soil C-destabilization and losses from soil following conversion. Soils in aquaculture ponds exhibited higher bulk density (BD; 11.3%) and lower levels of salinity (61.0%), soil water content (SWC; 11.7%), total nitrogen (TN) concentration (23.8%) and available-nitrogen concentration (37.7%; p < .05) than coastal-wetlands. Redundancy-analysis revealed that pH, BD and TN concentration were the key variables most linked with temporal variations in SOC fractions and stock between two land use types. This study provides a theoretical basis for the rational utilization and management of wetland resources, the achievement of an environment-friendly society, and the preservation of multiple service functions within wetland ecosystems.

Response of CH4 and N2O emissions to the feeding rates in a pond rice-fish co-culture system.

Feeding rate is an important factor influencing the carbon and nitrogen input and greenhouse gas emission from aquaculture systems. However, the quantitative relationship between feeding rates and GHG emissions is still poorly understood. In this study, we conducted a laboratory-scale experiment to examine the impact of feeding rate (0%, 2%, 4%, 6%, and 8%) on the CH4 and N2O emissions from a pond rice-fish co-culture system. The results showed that the total amount of CH4 emission did not significantly differ when the feeding rate was no more than 6%, but increased more than four times when the feeding rate reach to 8%. The amount of N2O emission showed a linearly increasing trend with the feeding rate. The emission factors of CH4 and N2O was significantly higher for 8% feeding rate than other feeding rates. The variation of CH4 emission was primarily attributed to the ratio of mcrA/pmoA in the sediment and the contents of biological oxygen demand (COD) and dissolved oxygen (DO) in the water; and the variation of N2O was primarily affected by the available nitrogen in the water and sediment and the content of DO in the water. The overall emission of CH4 and N2O showed an exponential relationship with feeding rate. The total yields of fish and rice did not continuously increase when the feeding rate exceeded 4%. The lowest emission intensity per unit yield was reached at the feeding rate of 2.99%. These results can provide a reference for the determination of low-carbon feeding strategy for pond rice-fish co-culture system.

Intraspecific priority effects in response to egg hatching delay in a pond-breeding salamander.

As reproduction phenologies shift with climate change, populations can experience intraspecific priority effects, wherein early hatching cohorts experience an advantage over late-hatching cohorts, resulting in altered demography. Our study objective was to identify how variation in egg hatching phenology alters intraspecific interactions in small-mouthed salamanders, Ambystoma texanum. We addressed two research questions: (Q1) How are demographic responses altered by variation in the temporal duration of hatching between cohorts, and (Q2) How does the seasonality of hatching delays affect demographic responses? We manipulated hatching phenologies of A. texanum eggs and reared larvae in outdoor mesocosms to metamorphosis. For Q1, hatching delay exhibited non-linear relationships with survival and body size, with the greatest asynchrony in cohort additions resulting in the highest mortality and largest body sizes. For Q2, hatching delay effects were stronger (i.e., survival was lower and body sizes larger) when they occurred later in the season, potentially due to temperature differences that larvae experienced. Overall, our results demonstrate that changes in intraspecific interactions due to phenological shifts can be context-dependent, depending on the strength (i.e., temporal duration) and seasonality of such processes. Identifying context-dependencies of phenological shifts will be critical for predicting changes in organismal demographics with climatic shifts.

Changes of vertical distribution of Microcystis colonies driven by short-term rainfall: Disappearance and reformation of surface bloom.

Global climate warming and human activities have increased the magnitude and frequency of Microcystis surface blooms, posing significant threats to freshwater ecosystems and human health over recent decades. Heavy rainfall events have been reported to cause the disappearance of these blooms. Although some studies have employed turbulence models to analyze the movement characteristics of Microcystis colonies, the impact of rainfall is complex, comprehensive investigations on their vertical migration induced by short-term rainfall are still necessary. Utilizing monitoring data from eutrophic ponds and controlled simulation experiments, this study examines the short-term impacts of rainfall on the vertical distribution of Microcystis in the water column. Our findings indicate that rainfall contributes to the disappearance of Microcystis blooms by reducing the quantity of small to medium-sized colonies (0-100 µm) at the surface, subsequently decreasing the overall Microcystis biomass. As rainfall intensity increases, larger colonies migrate deeper into the water column. At a rainfall threshold of 666 mm, the difference in the median volume diameter (DV50) of Microcystis colonies between the surface and bottom reaches a minimal value of 3.09%. Post-rainfall, these colonies rapidly ascend, aggregate into larger formations, and re-establish surface blooms. The greater the rainfall, the smaller the resultant Microcystis biomass, albeit with larger aggregated colony sizes. When rainfall exceeds 222 mm, the recovery rate of surface Microcystis biomass remains below 100%, decreasing to 19.48% at 666 mm of rainfall, while the median volume diameter (DV50) of the colonies increases to 139.07% of its pre-rainfall level. Furthermore, compared to pre-rainfall conditions, the photosynthetic activity of the surface Microcystis colonies was enhanced and the secretion of EPS was increased under heavy rainfall conditions. Our results identify a critical response time of 30 min for Microcystis colonies to rainfall, after which the response ceases to intensify. These insights are crucial for predicting post-rain Microcystis bloom dynamics and aiding management authorities in timely interventions.

Aquatic plants combined with microbial fuel cells promote sulfamethoxazole and sul genes removal from aquaculture pond sediments via bioelectrochemistry.

Antibiotics and antibiotic resistance genes (ARGs) in the aquaculture environment are receiving increasing public attention as emerging contaminants. In this study, aquatic plant (P) and sediment microbial fuel cells (SMFC) were used individually and in combination (P-SMFC) to simulate in situ remediation of sulfamethoxazole (SMX) and sul genes in aquaculture environments. The results showed that the average power densities of SMFC and P-SMFC were 622.18 mW m-2 and 565.99 mW m-2, respectively. The addition of 5 mg kg-1 of SMX to the sediment boosted the voltages of SMFC and P-SMFC by 36.3% and 51.5%, respectively. After 20 days of treatment, the removal efficiency of SMX from the sediment was 86.17% and 89.60% for SMFC and P-SMFC group, respectively, which were significantly higher than the control group (P < 0.05). However, removal of SMX by plants was not observed. P-SMFC group significantly reduced the biotoxicity of SMX to Staphylococcus aureus and Escherichia coli in the overlying water (P < 0.05). P and P-SMFC groups significantly reduced the abundance of ARGs intl1 and sul1 (P < 0.05). The removal rate of ARGs intl1, sul1 and sul2 from sediments by P-SMFC ranged from 94.22% to 97.08%. However, SMFC increased the abundance of sul3. SMFC and P-SMFC increased the relative abundance of some of sulfate-reducing bacteria such as Desulfatiglans, Thermodesulfovibrionia and Sva0485 in sediments. These results showed that aquatic plants promoted the removal of ARGs and SMFC promoted the removal of antibiotics, and the combination with aquatic plants and SMFC achieved a synergistic removal of both SMX and ARGs. Therefore, current study provides a promising approach for the in situ removal of antibiotics and ARGs in the aquaculture environment.

Exploration and Molecular Identification of Proteolytic Bacteria as Probiotic Candidates from Shrimp Ponds in West Sumatra, Indonesia.

<b>Background and Objective:</b> The existence of intensive shrimp aquaculture faces serious challenges in the form of a decrease in pond water quality due to overfeeding. Efforts are needed to improve pond water quality by utilizing proteolytic bacterial isolates to break down suspended or accumulated feed on the pond bottom. The research aims to find proteolytic bacterial isolates from pond sediments and the digestive tract of shrimp (<i>Litopenaeus vannamei</i>). <b>Materials and Methods:</b> The materials needed are pond sediment samples, shrimp digestive tract, seawater complete agar (SWCA) medium and skim milk agar medium (SMA). The study used survey methods to determine sampling locations and continued with experimental methods in the laboratory. Nine isolates were obtained from pond sediments and two bacterial isolates were from the shrimp digestive tract. <b>Results:</b> The proteolytic potency test showed that two isolates from pond sediments and one isolate from the digestive tract of shrimp were positive for proteolytic. The largest proteolytic index value reached 6.357. Molecular identification by analyzing the <i>16S rRNA</i> gene sequence shows that PC23 isolate is closely related to the bacterium <i>Exiguobacterium indicum </i>strain KR6 with percent identity 99.44-99.58% and PU32 isolate with <i>Bacillus cereus</i> strain 125 with percent identity 100%. <b>Conclusion:</b> The bacteria obtained can be used as probiotic candidates for the future are <i>Exiguobacterium indicum</i> strain KR6 and <i>Bacillus cereus</i> strain 125.

Pilot-scale microalgae cultivation and wastewater treatment using high-rate ponds: a meta-analysis.

Microalgae cultivation in wastewater has been widely researched under laboratory conditions as per its potential to couple treatment with biomass production. Currently, only a limited number of published articles consider outdoor and long-term microalgae-bacteria cultivations in real wastewater environmental systems. The scope of this work is to describe microalgal cultivation steps towards high-rate algal pond (HRAP) scalability and identify key parameters that play a major role for biomass productivity under outdoor conditions and long-term cultivations. Reviewed pilot-scale HRAP literature is analysed using multivariate analysis to highlight key productivity parameters within environmental and operational factors. Wastewater treatment analysis indicated that HRAP can effectively remove 90% of NH4+, 70% of COD, and 50% of PO43-. Mean reference values of 210 W m-2 for irradiation, 18 °C for temperature, pH of 8.2, and HRT of 7.7 are derived from pilot-scale cultivations. Microalgae biomass productivity at a large scale is governed by solar radiation and NH4+ concentration, which are more important than retention time variations within investigated studies. Hence, selecting the correct type of location and a minimum of 70 mg L-1 of NH4+ in wastewater will have the greatest effect in microalgae productivity. A high nutrient wastewater content increases final biomass concentrations but not necessarily biomass productivity. Pilot-scale growth rates (~ 0.54 day-1) are half those observed in lab experiments, indicating a scaling-up bottleneck. Microalgae cultivation in wastewater enables a circular bioeconomy framework by unlocking microalgal biomass for the delivery of an array of products.

Microplastic pollution and ecological risk assessment of a pond ecosystem.

Microplastic (MP) pollution has been observed in various ecosystems as a result of the rapid increase in plastic production over the past half-century. Nevertheless, the extent of MP pollution in different ecosystems, particularly in freshwater ecosystems, has not been well-studied, and there are limited investigations on this particular topic, specifically in Türkiye. Here, we quantify the occurrence and distribution of MPs in surface water samples collected from Topçu Pond (Türkiye) for the first time. Water samples were collected at five stations and filtered (30 L for each station) through stacked stainless steel sieves (5 mm, 328 µm, and 61 µm mesh size) with a diameter of 30 cm. The abundance, size, color, shape, and type of collected debris samples were analyzed after the wet peroxide oxidation process. MP particles were observed in all samples at an average abundance of 2.4 MPs/L. The most abundant MP size class and type were 0-999 µm and fiber respectively. On the other hand, prevalent colors were black and colorless in general. According to the Raman analysis results, the identified MP derivatives were polypropylene (40%), polyamide (30%), ethylene acrylic acid (20%), and polyvinylchloride (10%). Moreover, the pollution load index (PLI) index was used to determine the pollution status. PLI values were determined as 1.91 at station S1, 1.73 at station S2, 1.31 at station S3, 1 at station S4 and 1.24 at station S5. The PLI value determined for the overall pond was 1.4. The results of this research show that MP pollution is present in Topçu Pond and contributes to the expanding literature on MP pollution in pond ecosystems.

Control of cyanobacterial growth with potassium; implications for bloom control in nuclear storage ponds.

Microbial blooms have been reported in the First Generation Magnox Storage Pond at the Sellafield Nuclear Facility. The pond is kept alkaline with NaOH to minimise fuel rod corrosion, however alkali-tolerant microbial blooms dominated by the cyanobacterium Pseudanabaena catenata are able to thrive in this hostile environment. This study assessed the impact of alternative alkali-dosing regimens (KOH versus NaOH treatment) on biomass accumulation, using a P. catenata dominated mixed culture, which is representative of the pond environment. Optical density was reduced by 40-67 % with KOH treatment over the 3-month chemostat experiment. Microbial community analysis and proteomics demonstrated that the KOH-dependent inhibition of cell growth was mostly specific to P. catenata. The addition of KOH to nuclear storage ponds may therefore help control growth of this pioneer photosynthetic organism due to its sensitivity to potassium, while maintaining the high pH needed to inhibit the corrosion of stored nuclear fuel.

Food web collapse and regime shift following goldfish introduction in permanent ponds.

In a global context of invasive alien species (IAS), native predators are often eradicated by functionally different IAS, which may induce complex cascading consequences on ecosystem functioning because of the key role predators play in structuring communities and stabilizing food webs. In permanent ponds, the most abundant freshwater systems on Earth, global human-mediated introductions of alien omnivores such as the pet trade goldfish are driving broad-scale patterns of native predators' exclusion, but cascading consequences on food web structure and functioning are critically understudied. We compared food webs of naturally fishless ponds versus ponds where dominant native predators (newts) had been extirpated by invasive goldfish within the last decade. Integrating community-wide isotopic, taxonomic and functional traits approaches, our study reveals that pond food webs collapsed in both vertical and horizontal dimensions following goldfish introduction and the associated exclusion of native predators. Consumer taxonomic diversity was drastically reduced, essentially deprived of amphibians as well as predatory and mobile macroinvertebrates to the profit of burrowing, lower trophic level consumers (detritivores). Changes in community structure and function underlined a regime shift from a macrophyte-dominated system mainly characterized by benthic primary production (periphyton), to a macrophyte-depleted state of ponds hosting communities mainly associated with phytoplankton primary production and detritus accumulation, with higher tolerance to eutrophication and low dissolved oxygen concentration. Results underline major impacts of widely introduced omnivores such as the goldfish on the functioning of pond ecosystems with potentially dramatic consequences on the key ecosystem services they deliver, such as global biodiversity support or water quality improvement. They also shed light on the key role of submerged aquatic vegetation in supporting diverse communities and complex food webs in shallow lentic systems and call for urgent consideration of threats posed by IAS on ponds' ecosystems by managers and policymakers.

Trophic effects of Bti-based mosquito control on two top predators in floodplain pond mesocosms.

Chironomid (Diptera: Chironomidae) larvae play a key role in aquatic food webs as prey for predators like amphibian and dragonfly larvae. This trophic link may be disrupted by anthropogenic stressors such as Bacillus thuringiensis var. israelensis (Bti), a biocide widely used in mosquito control. In a companion study, we recorded a 41% reduction of non-target larval chironomids abundance in outdoor floodplain pond mesocosms (FPMs) treated with Bti. Therefore, we examined the diet of two top predators in the FPMs, larvae of the palmate newt (Salamandridae: Lissotriton helveticus) and dragonfly (Aeshnidae: predominantly Anax imperator), using bulk stable isotope analyses of carbon and nitrogen. Additionally, we determined neutral lipid fatty acids in newt larvae to assess diet-related effects on their physiological condition. We did not find any effects of Bti on the diet proportions of newt larvae and no significant effects on the fatty acid content. We observed a trend in Aeshnidae larvae from Bti-FPMs consuming a higher proportion of large prey (Aeshnidae, newt, damselfly larvae; ~42%), and similar parts of smaller prey (chironomid, mayfly, Libellulidae, and zooplankton), compared to controls. Our findings may suggest bottom-up effects of Bti on aquatic predators but should be further evaluated, for instance, by using compound-specific stable isotope analyses of fatty acids or metabarcoding approaches.

Acclimatization of a sequencing batch vertical oxidation pond with simulated agricultural wastewater using duckweed as vegetation: analysis of efficiency, Biomass, and Soil properties.

Vertical oxidation pond operated in sequencing batch mode (HRT: 1.25 day) with duckweed as the vegetation was used to acclimatize with simulated agricultural wastewater. The maximum removal rate of urea [371 g/(m3.d)] and COD [222.4 g/(m3.d)] were observed at moderate concentrations of urea (500 mg/L), N-P-K (60 mg/L), and pesticide (20 mg/L). Inhibition and toxicity posed by higher concentrations, decreased the removals of urea (83% to 61%), COD (81% to 51%), and TDS (76% to 50%) at the end of the acclimatization. Steady removal (> 99%) of PO43--P was observed during acclimatization. Effluent pH increased due to the generation of NH4+-N (maximum 370 ± 5 mg/L) from the assimilation of urea. Oxidation of ammonia led to the maximum generation of NO2--N and NO3--N of 10 mg/L and 9 mg/L, respectively. Particles less than 300 µm increased, and both specific gravity (from 2.62 to 2.42) and maximum dry density (from 1.73 to 1.30 g/cm3) of the base soil decreased with an increase in urea, N-P-K, and pesticide. Reactor biomass increased (1.42 to 1.90 g/L) up to initial concentrations of urea (500 mg/L), N-P-K (60 mg/L), and pesticide (20 mg/L), then decreased (1.68 g/L) with an increase in concentration.

Diminished storage capacity of ponds caused by sedimentation weakens their nitrogen removal efficiency.

Though their small size, ponds play a disproportionately crucial role in eliminating nitrogen (N) transporting to downstream freshwaters. As significant water infrastructures, ponds are non-sustainable due to loss of storage capacity resulting from sedimentation. However, the effects of pond sedimentation on N removal is widely neglected in landscape N processing. The NUFER (Nutrient flows in Food chains, Environment and Resources use) model was employed to estimate N runoff from 1960 to 2018. We reconstructed the dynamic of number and storing capacity of about 14 million ponds due to construction and sedimentation from 1960 to 2018, projecting these trends into the year 2060. Our approach incorporated first-order kinetic reactions, including water residence time (HRT), to estimate N removal of ponds, utilizing data 6 monitoring ponds and 81 ponds from literature studies. Our analysis reveals a fourteen-fold increase in N runoff over the past six decades, rising from 0.8 Mt N in 1960 to 11.4 Mt N in 2018. Due to the initial rapid expansion of ponds, N removal by ponds increased from 6.4 % in 1960 to 13.6 % in 1990. Sedimentation is prevalent in ponds, particularly in small ponds with a sedimentation accumulation rate of 2.96 cm yr-1. Pond sedimentation, which reduces HRT, resulted in a decrease in pond N removal percentage to 11.2 % in 2018 and a projected 7.4 % by the year 2060, assuming similar sediment accumulation rates persist in the future. Overall, our findings underscore the non-negligible role of ponds as landscape nodes in N cycling. Urgent mitigation measures are needed to extend the lifetime of existing ponds and sustain their critical role in water quality management.

Improving denitrification estimation by joint inclusion of suspended particles and chlorophyll a in aquaculture ponds.

The denitrification process in aquaculture systems plays a crucial role in nitrogen (N) cycle and N budget estimation. Reliable models are needed to rapidly quantify denitrification rates and assess nitrogen losses. This study conducted a comparative analysis of denitrification rates in fish, crabs, and natural ponds in the Taihu region from March to November 2021, covering a complete aquaculture cycle. The results revealed that aquaculture ponds exhibited higher denitrification rates compared to natural ponds. Key variables influencing denitrification rates were Nitrate nitrogen (NO3--N), Suspended particles (SPS), and chlorophyll a (Chla). There was a significant positive correlation between SPS concentration and denitrification rates. However, we observed that the denitrification rate initially rose with increasing Chla concentration, followed by a subsequent decline. To develop parsimonious models for denitrification rates in aquaculture ponds, we constructed five different statistical models to predict denitrification rates, among which the improved quadratic polynomial regression model (SQPR) that incorporated the three key parameters accounted for 80.7% of the variability in denitrification rates. Additionally, a remote sensing model (RSM) utilizing SPS and Chla explained 43.8% of the variability. The RSM model is particularly valuable for rapid estimation in large regions where remote sensing data are the only available source. This study enhances the understanding of denitrification processes in aquaculture systems, introduces a new model for estimating denitrification in aquaculture ponds, and offers valuable insights for environmental management.

Duplex Real-Time Fluorescent Quantitative PCR Assays for the Detection of Toxigenic Microcystis Genotypes Based on SNP/InDel Variation in mcy Gene Cluster.

In this study, the toxigenic characteristics of 14 strains of Microcystis were analyzed, and single nucleotide polymorphism (SNP) and insertion/deletion (InDel) loci in microcystin synthetase (mcy) gene clusters were screened. Based on SNP and InDel loci associated with the toxigenic characteristics, primers and TaqMan or Cycling fluorescent probes were designed to develop duplex real-time fluorescent quantitative PCR (FQ-PCR) assays. After evaluating specificity and sensitivity, these assays were applied to detect the toxigenic Microcystis genotypes in a shrimp pond where Microcystis blooms occurred. The results showed a total of 2155 SNP loci and 66 InDel loci were obtained, of which 12 SNP loci and 5 InDel loci were associated with the toxigenic characteristics. Three duplex real-time FQ-PCR assays were developed, each of which could quantify two genotypes of toxigenic Microcystis. These FQ-PCR assays were highly specific, and two Cycling assays were more sensitive than TaqMan assay. In the shrimp pond, six genotypes of toxigenic Microcystis were detected using the developed FQ-PCR assays, indicating that above genotyping assays have the potential for quantitative analysis of the toxigenic Microcystis genotypes in natural water.

Microbiomes on microplastics versus natural microcarriers: Stability and transformation during aquatic travel from aquaculture ponds to adjacent stream.

Microplastics (MPs) with different physical-chemical properties are considered as vectors for the propagation of microbes in aquatic environments. It remains unclear how plastic types impact on the plastisphere and whether different MPs spread microbes more rapidly than natural materials in microbes across distinct water bodies as proposed previously. We used in-situ incubation to investigate the microbes attached on MPs of polyethylene (PE), polypropylene (PP), and polyvinyl chloride (PVC), versus that on two natural microcarriers (quartz sands and bamboo) during the travel from aquaculture ponds with impacted by fish farming to adjacent freshwater stream. The results showed that the microbial communities on the carriers were shaped not only by environmental conditions, which were primary determinants but also by carrier types. All the tested plastics did not carry more microbes than the natural carriers during the journey. The biofilm community composition on PVC is distinct from that on PE and PP MPs and natural carriers. The plastisphere of PE and PP kept microbial proportions as natural materials did but PVC retained less than nature materials. Bamboo carried more potential pathogens than plastic polymers and quartz. The results indicated that the communities of plastisphere is polymer-type dependent, and, compared with the natural materials, MPs did not show enhanced propagation of microbes, including pathogens, cross distinct environments.

Assessing controls on sedimentation rates and sediment organic carbon accretion in beaver ponds.

Beaver dams trap sediment, promote channel-floodplain connectivity, modify biogeochemical cycling and organic carbon (OC) storage, and influence geomorphic form. Beaver-related sediment accumulation has been investigated at longer timescales (e.g., > 1000 years) and shorter timescales (< 10 years), but we lack information on sedimentation and sediment-associated OC accretion rates over multiple decades in relatively persistent beaver ponds (10-100 years old). We coupled field surveys of 45 beaver ponds with historical aerial imagery and radiometric dating with 7Be, 210Pb, and 14C to calculate sedimentation rates, mean sediment depth, and sediment OC content at two study sites in the southern Rocky Mountains, USA. Sedimentation rates in beaver ponds (median = 5.7 cm yr-1, mean = 11.6 cm yr-1) decreased with pond age. Incised, single threaded reaches had greater variability in mean sediment depth compared to less incised reaches. In less incised reaches, mean sediment depth and beaver dam height increased with pond age, indicating more stable dams and depositional environments. Sediment OC content within beaver ponds (median = 0.8 %, mean = 1.7 %) increased with finer sediment grain size distributions. Sediment OC accretion rates in ponds ranged between 0.13 and 23 Mg C ha -1 per year. We used Monte Carlo simulations to estimate it would take ∼100 years or more of uninhibited beaver activity for deposition to laterally reconnect adjacent terraces in the incised study reaches, a common objective within many stream restoration projects. Our findings show that beaver ponds in complex, multi-threaded reaches better retain fine sediment over longer timescales, highlighting the need to incorporate geomorphic context when considering whether beaver can help restore incised river channels and floodplain connectivity, retain fine sediment, and store OC on the landscape.

Cyanobacteria respond to trophic status in shallow aquatic ecosystems.

Small and shallow water bodies are particularly sensitive to adverse conditions connected with anthropogenic eutrophication. As model systems, ponds are a good object for ecological research and monitoring of global environmental changes. We examined cyanobacteria along with other groups of algae versus zooplankton and abiotic characteristics of water in 51 aquatic ecosystems exposed to anthropogenic pressure (from natural forest to highly disturbed field ponds) with 3 distinct trophic groups: meso-, eu- and hypertrophic. This study aimed to define how different levels of trophy affect pond-specific cyanobacteria assemblages and to identify species responding to particular trophic states. We demonstrated that trophic type determined the occurrence of certain cyanobacteria species. From among 78 identified taxa, shade- and turbid mixed adapted were the most numerous. Eutrophic ponds had the highest cyanobacteria species and diversity and abundance of zooplankon. Dominating species such as Chroococcus minimus, Anagnostidinema amphibium, Phormidium granulatum or Komvophoron minutum preferred mesotrophic, while e.g. Jaaginema subtilissimum, Limnolyngbya circumcreta, Limnothrix vacuolifera or Romeria leopolienis eutrophic waters and these were not grazed by filtrators. Only 3 species (Aphanizomenon flos-aquae, Dolichospermum circinale, Planktothrix agardhii) were associated with hypertrophic ponds. Therefore, we assume that cyanobacteria taxa have a high indicative potential to distinguish between trophic type of ponds. Reynolds Functional Groups also exhibit responses to changes in water quality. It was partucularly evident in the case of cyanobacteria representatives of codon M which was attributed to eutrophic ponds. Advancing our understanding about trophic preferences of cyanobacteria is crucial, especially in the era of global warming and the persistent issue of water eutrophication, when problems with harmful cyanobacterial blooms are intensifying. The research findings have ecological significance and management implications, highlighting the often-overlooked importance of pond ecosystems in maintaining overall water quality.