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1.
Food Microbiol ; 124: 104600, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39244359

RESUMO

This study aimed to assess the impact of Saccharomyces cerevisiae and different non-Saccharomyces cerevisiae (Zygosaccharomyces bailii, Hanseniaspora opuntiae and Zygosaccharomyces rouxii) on the volatile compounds and sensory properties of low-alcohol pear beverages fermented from three varieties of pear juices (Korla, Laiyang and Binzhou). Results showed that all three pear juices were favorable matrices for yeasts growth. Non-Saccharomyces cerevisiae exhibited a higher capacity for acetate ester production compared to Saccharomyces cerevisiae, resulting in a significant enhancement in sensory complexity of the beverages. PCA and sensory analysis demonstrated that pear varieties exerted a stronger influence on the crucial volatile components and aroma characteristics of the fermented beverages compared to the yeast species. CA results showed different yeast strains exhibited suitability for the fermentation of specific pear juice varieties.


Assuntos
Fermentação , Odorantes , Pyrus , Saccharomyces cerevisiae , Compostos Orgânicos Voláteis , Compostos Orgânicos Voláteis/metabolismo , Compostos Orgânicos Voláteis/análise , Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/crescimento & desenvolvimento , Pyrus/microbiologia , Pyrus/química , Odorantes/análise , Sucos de Frutas e Vegetais/análise , Sucos de Frutas e Vegetais/microbiologia , Paladar , Humanos , Zygosaccharomyces/metabolismo , Zygosaccharomyces/crescimento & desenvolvimento , Hanseniaspora/metabolismo , Hanseniaspora/crescimento & desenvolvimento , Frutas/microbiologia , Frutas/química , Saccharomycetales
2.
Food Microbiol ; 124: 104610, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39244362

RESUMO

This study aimed to assess the impact of adaptation of ten strains of O157:H7 and non-O157 Escherichia coli to low pH (acid shock or slow acidification) and the effects of this exposure or not on the resistance of E. coli strains to UV radiation in orange juice (pH 3.5). The acid-shocked cells were obtained through culture in tryptic soy broth (TSB) with a final pH of 4.8, which was adjusted by hydrochloric, lactic, or citric acid and subsequently inoculated in orange juice at 4 °C for 30 days. No significant differences (p > 0.05) in survival in orange juice were observed between the serotypes O157:H7 and non-O157:H7 for acid-shocked experiments. After slow acidification, where the cells were cultured in TSB supplemented with glucose 1% (TSB + G), a significant increase (p < 0.05) in survival was observed for all strains evaluated. The D-values (radiation dose (J/cm2) necessary to decrease the microbial population by 90%) were determined as the inverse of the slopes of the regressions (k) obtained by plotting log (N/N0). The results show that among the strains tested, E. coli O157:H7 (303/00) and O26:H11 were the most resistant and sensitive strains, respectively. According to our results, the method of acid adaptation contributes to increasing the UV resistance for most of the strains tested.


Assuntos
Adaptação Fisiológica , Citrus sinensis , Escherichia coli O157 , Sucos de Frutas e Vegetais , Raios Ultravioleta , Escherichia coli O157/efeitos da radiação , Escherichia coli O157/crescimento & desenvolvimento , Escherichia coli O157/efeitos dos fármacos , Sucos de Frutas e Vegetais/microbiologia , Sucos de Frutas e Vegetais/análise , Citrus sinensis/microbiologia , Citrus sinensis/química , Concentração de Íons de Hidrogênio , Escherichia coli/efeitos da radiação , Escherichia coli/efeitos dos fármacos , Ácidos/farmacologia , Contagem de Colônia Microbiana , Microbiologia de Alimentos , Viabilidade Microbiana/efeitos da radiação , Viabilidade Microbiana/efeitos dos fármacos , Irradiação de Alimentos
3.
Food Microbiol ; 124: 104611, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39244364

RESUMO

The quality and sensory attributes of juices are influenced by their natural microbiota and the microorganisms found on filtration membranes. This study aimed to assess the influence of natural microbiota and specific contaminants, including Candida krusei, Rhodotorula mucilaginosa, Debaryomyces prosopidis, Ralstonia insidiosa, and Lactiplantibacillus paraplantarum, isolated from cranberry juice and its associated industrial filtration membranes, on the characteristics of cranberry juice. Their growth kinetics and impacts on total phenols, total anthocyanins, total proanthocyanins, total organic acids, pH, titratable acidity, and volatile compounds were assessed. During the 42 h fermentation period, Candida krusei and Ralstonia insidiosa exhibited significant growth, increasing by 1-log and 3-log, respectively. The natural microbiota led to a 7% and 6% reduction in anthocyanins and proanthocyanidins, while Candida krusei and Rhodotorula mucilaginosa caused losses of 10% and 7% in proanthocyanidins, respectively. Organic acid content remained stable, except for an 8% decrease caused by Ralstonia insidiosa. Volatile compounds underwent significant increases, particularly in green (703%), winey (100%), mushroom (306%), and fusel (2678%) notes. These findings underscore the rapid impact of microorganisms from natural microbiota and filtration membranes on cranberry juice characteristics, highlighting the importance for beverage industries to prioritize customer safety and satisfaction.


Assuntos
Manipulação de Alimentos , Sucos de Frutas e Vegetais , Microbiota , Proantocianidinas , Vaccinium macrocarpon , Compostos Orgânicos Voláteis , Vaccinium macrocarpon/química , Vaccinium macrocarpon/microbiologia , Sucos de Frutas e Vegetais/microbiologia , Sucos de Frutas e Vegetais/análise , Compostos Orgânicos Voláteis/análise , Compostos Orgânicos Voláteis/metabolismo , Compostos Orgânicos Voláteis/química , Proantocianidinas/análise , Odorantes/análise , Fermentação , Bactérias/classificação , Bactérias/isolamento & purificação , Bactérias/crescimento & desenvolvimento , Bactérias/metabolismo , Antocianinas/análise , Candida/crescimento & desenvolvimento , Fungos/classificação , Fungos/metabolismo , Fungos/isolamento & purificação , Fungos/crescimento & desenvolvimento
4.
Rapid Commun Mass Spectrom ; 38(22): e9906, 2024 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-39226917

RESUMO

RATIONALE: The oxygen stable isotope ratio (δ18O) of the sugar-rich fraction of fruit juice is important as a tracer of the geographical origin of raw material. This study sought to minimize the inter-day variation of δ18O attributable to the influence of water to accurately monitor geographical origin labeling. METHODS: Two drying devices (freeze dryer and vacuum oven) were compared. Then, two humidity levels (normal and low humidity) at which the samples were placed after drying were compared. The low-humidity environment was constructed using a glove bag and pure argon gas. δ18O was measured using thermal conversion elemental analyzer/isotope ratio mass spectrometry. Improvements were made to the measurement method based on aforementioned analyses results, and the performance of the initial and improved methods was compared. RESULTS: δ18O of juice dried in a vacuum oven was 3.30‰ lower than that of juice dried in a freeze dryer. Moreover, δ18O of juice samples exposed to normal humidity was 3.74‰ lower than that of samples exposed to low humidity. The combined inter-day and intra-day standard deviation was reduced from 1.20‰ in the initial method to 0.42‰ in the improved method. CONCLUSIONS: This study describes a pretreatment method for δ18O measurement in the sugar-rich fraction of fruit juice with less inter-day variation, and it will be useful for monitoring geographical origin labeling.


Assuntos
Sucos de Frutas e Vegetais , Espectrometria de Massas , Isótopos de Oxigênio , Isótopos de Oxigênio/análise , Sucos de Frutas e Vegetais/análise , Espectrometria de Massas/métodos , Umidade , Açúcares/análise , Açúcares/química
5.
Nutrients ; 16(17)2024 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-39275212

RESUMO

Microbial dysbiosis may manifest as inflammation both orally and in the gastrointestinal tract. Altered oral and gut microbiota composition and decreased diversity have been shown in inflammatory bowel disease (IBD) and periodontal disease (PD). Recent studies have verified transmission of oral opportunistic microbes to the gut. Prebiotics, probiotics, or dietary interventions are suggested to alleviate IBD symptoms in addition to medicinal treatment. Lingonberries contain multiple bioactive molecules, phenolics, which have a broad spectrum of effects, including antimicrobial, anti-inflammatory, antioxidant, anti-proteolytic, and anti-cancer properties. An all-natural product, fermented lingonberry juice (FLJ), is discussed as a potential natural anti-inflammatory substance. FLJ has been shown in clinical human trials to promote the growth of oral lactobacilli, and inhibit growth of the opportunistic oral pathogens Candida, Streptococcus mutans, and periodontopathogens, and decrease inflammation, oral destructive proteolysis (aMMP-8), and dental microbial plaque load. Lactobacilli are probiotic and considered also beneficial for gut health. Considering the positive outcome of these oral studies and the fact that FLJ may be swallowed safely, it might be beneficial also for the gut mucosa by balancing the microbiota and reducing proteolytic inflammation.


Assuntos
Anti-Inflamatórios , Sucos de Frutas e Vegetais , Microbioma Gastrointestinal , Doenças Inflamatórias Intestinais , Humanos , Anti-Inflamatórios/administração & dosagem , Microbioma Gastrointestinal/efeitos dos fármacos , Doenças Inflamatórias Intestinais/microbiologia , Doenças Inflamatórias Intestinais/dietoterapia , Fermentação , Vaccinium vitis-Idaea , Boca/microbiologia , Probióticos/administração & dosagem , Disbiose , Simbiose
6.
Nutrients ; 16(17)2024 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-39275255

RESUMO

Non-alcoholic fatty liver disease (NAFLD) is a common chronic liver disorder marked by the buildup of triacylglycerols (TGs) in the liver. It includes a range of conditions, from simple steatosis to more severe forms like non-alcoholic steatohepatitis (NASH), which can advance to fibrosis, cirrhosis, and hepatocellular carcinoma. NAFLD's prevalence is rising globally, estimated between 10% and 50%. The disease is linked to comorbidities such as obesity, type 2 diabetes, insulin resistance, and cardiovascular diseases and currently lacks effective treatment options. Therefore, researchers are focusing on evaluating the impact of adjunctive herbal therapies in individuals with NAFLD. One herbal therapy showing positive results in animal models and clinical studies is fruits from the Vaccinium spp. genus. This review presents an overview of the association between consuming fruits, juices, and extracts from Vaccinium spp. and NAFLD. The search used the following keywords: ((Vaccinium OR blueberry OR bilberry OR cranberry) AND ("non-alcoholic fatty liver disease" OR "non-alcoholic steatohepatitis")). Exclusion criteria included reviews, research notes, book chapters, case studies, and grants. The review included 20 studies: 2 clinical trials and 18 studies on animals and cell lines. The findings indicate that juices and extracts from Vaccinium fruits and leaves have significant potential in addressing NAFLD by improving lipid and glucose metabolism and boosting antioxidant and anti-inflammatory responses. In conclusion, blueberries appear to have the potential to alleviate NAFLD, but more clinical trials are needed to confirm these benefits.


Assuntos
Frutas , Hepatopatia Gordurosa não Alcoólica , Extratos Vegetais , Vaccinium , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/terapia , Humanos , Frutas/química , Vaccinium/química , Animais , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Fitoterapia , Sucos de Frutas e Vegetais
7.
Food Res Int ; 195: 114997, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39277257

RESUMO

Repeated urinary tract infections affect many people worldwide. A potential strategy to reduce the incidence of these infections is to consume probiotics and cranberry fruit regularly. In this context, this study aims to prepare fermented milk with Lactobacillus acidophilus La-5 added with concentrated cranberry juice in two concentrations (5 and 10 %, corresponding to C1 and C2 samples, respectively) and evaluate different technological aspects of the samples after production and during storage, and comparing with the control sample (C). The juice had pH 1.91, 70.09 % of solids, and total proanthocyanidins and A-type proanthocyanidins (PACs) values of 117.03 mg/100 g and 16.38 mg/100 g, respectively. The higher the juice content added to the product, the higher the acidity (1.4 and 2.6 g of lactic acid in 100 g, corresponding to C1 and C2 on day 1 (D1), respectively), the total proanthocyanidin content (1.96 and 4.01 mg/100 g on D1; and 1.31 and 3.05 mg/100 g on day 28 of storage (D28), corresponding to C1 and C2, respectively) and A-type proanthocyanidin (0.56 and 1.26 mg/100 g in Day 1; and 0.54 and 1.19 mg/100 g in D28, corresponding to C1 and C2, respectively), higher the values of the color parameters (L*a* and C*), and lower pH value, probiotic viability, and sensory acceptance. Furthermore, the rheological parameters demonstrated a stronger protein network due to the addition of cranberry. The new formulations, including samples C1 and C2, are alternatives as functional products, which regular consumption probably has the potential to minimize the recurrence of urinary tract infections.


Assuntos
Produtos Fermentados do Leite , Sucos de Frutas e Vegetais , Lactobacillus acidophilus , Proantocianidinas , Infecções Urinárias , Vaccinium macrocarpon , Vaccinium macrocarpon/química , Infecções Urinárias/prevenção & controle , Infecções Urinárias/microbiologia , Produtos Fermentados do Leite/microbiologia , Humanos , Probióticos , Fermentação , Concentração de Íons de Hidrogênio , Recidiva
8.
Molecules ; 29(17)2024 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-39274900

RESUMO

The effect of different methods of drying celery root enriched with beet juice by vacuum impregnation (VI) was studied. The process of convection drying, vacuum drying and freeze drying was carried out. Compared to dried indigenous celery, dry impregnated tissue was characterized by lower values of dry matter, L* and b* color parameters, as well as higher values of water activity, density and a* color parameter. In addition, VI reduced the drying time. Forty Volatile Organic Compounds (VOCs) were found in celery, while fifty-one VOCs were found in the profile of celery with beetroot juice. The innovative method of vacuum impregnation made it possible to produce a new type of product with changed properties and a variable VOCs profile. The best fit of the drying process kinetics was achieved by using the logistic model. Increasing the temperature during convection drying resulted in shorter drying time, increased values of dry matter, reduced the water activity value and altered VOCs.


Assuntos
Apium , Beta vulgaris , Dessecação , Compostos Orgânicos Voláteis , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/análise , Beta vulgaris/química , Cinética , Dessecação/métodos , Apium/química , Sucos de Frutas e Vegetais/análise , Raízes de Plantas/química , Liofilização/métodos , Água/química
9.
J Clin Pediatr Dent ; 48(5): 131-137, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39275830

RESUMO

Recently, interest in tooth-colored fluoride-releasing dental materials has increased. Although physical and mechanical properties such as surface hardness, elastic modulus and surface roughness of the restorative materials have been investigated, the effect of different immersion media on these properties is still controversial. The aim of this study was to evaluate the nanohardness, elastic modulus and surface roughness of the fluoride release of tooth-colored restorative materials after immersion in acidic beverages. Prepared samples of three restorative materials (a highly viscous glass ionomer (EQUIA Forte; GC, Tokyo, Japan), a compomer (Dyract XP; Dentsply, Weybridge, UK), and a bioactive restorative material (Activa BioACTIVE; Pulpdent, MA, USA)) were randomly divided and immersed in distilled water, a cola and an orange juice for one week. The HYSITRON T1 950 TriboIndenter device (Hysitron, USA) with the Berkovich diamond indenter tip was used for all measurements. The nanohardness and elastic modulus of the samples were measured by applying a force of 6000 µN to five different points on the sample surface. Surface roughness measurements were evaluated on random samples by scanning five random 40 × 40 µm areas. The properties were measured at the initial and one week after immersion. The values of nanohardness, elastic modulus and surface roughness were tested for significant differences using a two-way analysis of variance (ANOVA) with repeated measures (p < 0.05). Tukey's honest significant difference (HSD) test was used for multiple comparisons. AB (Activa BioACTIVE) had the highest initial mean values for nanohardness. After post-immersion, the highest mean value for elastic modulus was the initial AB value. The lowest mean value for roughness of 100.36 nm was obtained for the initial DX (Dyract XP) measurement. Acidic beverages had a negative effect on the nanohardness, elastic modulus and surface roughness of the restorative materials.


Assuntos
Bebidas Gaseificadas , Módulo de Elasticidade , Fluoretos , Dureza , Teste de Materiais , Propriedades de Superfície , Fluoretos/química , Compômeros/química , Humanos , Materiais Dentários/química , Sucos de Frutas e Vegetais , Resinas Acrílicas/química , Restauração Dentária Permanente/métodos , Água/química , Cariostáticos/química , Cimentos de Ionômeros de Vidro/química , Citrus sinensis/química , Imersão , Resinas Compostas/química , Dióxido de Silício
10.
BMC Res Notes ; 17(1): 249, 2024 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-39237963

RESUMO

OBJECTIVES: Apples are one of the most frequently consumed fruits and are effective in preventing lifestyle-related and other diseases. However, few studies have been conducted to evaluate health benefits of processed apple products such as juice. In this study, we analyzed the health benefits of consuming apple juice, focusing on changes in the gut microbiota, which plays an important role in maintaining human health. RESULTS: Rats were fed apple juice ad libitum, and the relative abundances of various gut microbiota in fecal samples were analyzed. In addition, rats treated apple juice were fed with a high-fat diet, and body weight, plasma triglyceride, glucose, and cholesterol levels were measured. The relative abundance of Clostridium cluster XIV did not change with the treatment of apple juice, but the relative abundance of Clostridium cluster IV was significantly decreased. In contrast, the relative abundances of Lactobacillus and Bifidobacterium, which provide benefits to the human body, were significantly increased by 3-fold and 10-fold, respectively, with apple juice consumption. When apple juice-treated rats were fed a high-fat diet, the increase in body weight, liver fat, and blood lipid parameters were all suppressed compared to high-fat alone group. CONCULUSION: This study suggests that the consumption of apple juice changes the gut microbiota, exerts a prebiotic effect, and is effective in improving lifestyle-related diseases.


Assuntos
Dieta Hiperlipídica , Sucos de Frutas e Vegetais , Microbioma Gastrointestinal , Malus , Prebióticos , Animais , Microbioma Gastrointestinal/efeitos dos fármacos , Ratos , Masculino , Prebióticos/administração & dosagem , Peso Corporal/efeitos dos fármacos , Ratos Sprague-Dawley , Fezes/microbiologia , Triglicerídeos/sangue , Bifidobacterium
11.
Cell Physiol Biochem ; 58(4): 445-457, 2024 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-39230349

RESUMO

BACKGROUND/AIMS: Lemons (Citrus limon ) contain various nutrients and are among the most popular citrus fruit. Besides their antioxidant, anticancer, antibacterial, and anti-inflammatory properties, clinical studies have indicated their anti-allergic properties. METHODS: Using the differential-interference contrast (DIC) microscopy, we examined the effects of lemon juice and peel constituents, such as citric acid, ascorbic acid, hesperetin and eriodictyol, on the degranulation from rat peritoneal mast cells. Using fluorescence imaging with a water-soluble dye, Lucifer Yellow, we also examined their effects on the deformation of the plasma membrane. RESULTS: Lemon juice dose-dependently decreased the number of degranulated mast cells. At concentrations equal to or higher than 0.25 mM, citric acid, hesperetin, and eriodictyol significantly reduced the number of degranulating mast cells in a dose-dependent manner, while ascorbic acid required much higher doses to exert significant effects. At 1 mM, citric acid, hesperetin, and eriodictyol almost completely inhibited exocytosis and washed out the Lucifer Yellow trapped on the mast cell surface, while ascorbic acid did not. CONCLUSION: This study provides in vitro evidence for the first time that lemon constituents, such as citric acid, hesperetin, and eriodictyol, potently exert mast cell-stabilizing properties. These properties are attributable to their inhibitory effects on plasma membrane deformation in degranulating mast cells.


Assuntos
Ácido Ascórbico , Citrus , Flavanonas , Hesperidina , Mastócitos , Animais , Mastócitos/efeitos dos fármacos , Mastócitos/metabolismo , Citrus/química , Ratos , Ácido Ascórbico/farmacologia , Masculino , Hesperidina/farmacologia , Hesperidina/química , Flavanonas/farmacologia , Flavanonas/química , Ácido Cítrico/farmacologia , Ácido Cítrico/química , Degranulação Celular/efeitos dos fármacos , Sucos de Frutas e Vegetais/análise , Peritônio/citologia , Ratos Sprague-Dawley , Exocitose/efeitos dos fármacos , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Frutas/química , Isoquinolinas
12.
J Nutr Sci Vitaminol (Tokyo) ; 70(4): 359-363, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39218698

RESUMO

Ascorbic acid is essential for human health. As this vitamin is water-soluble, it cannot be stored in the body for a long time and is easily excreted in urine; therefore, it is necessary to ingest it in sufficient amounts every day. The fact that apples retain ascorbic acid in human bodies are known; however, this has not been experimentally demonstrated/documented. In this study, to clarify the effect of apple juice ingestion on the urinary excretion of ascorbic acid, we compared urinary ascorbic acid excretion in healthy women administered ascorbic acid alone or with apple juice. The experimental design was an unblinded randomized crossover study. Subjects ingested ascorbic acid in apple juice or ascorbic acid with water. Urine was collected after ingestion, and urinary ascorbic acid was measured. When ascorbic acid was ingested with apple juice, urinary excretion of ascorbic acid was significantly suppressed compared to when ascorbic acid was ingested alone. This suggests that apple juice intake can help retain ascorbic acid in the body.


Assuntos
Ácido Ascórbico , Estudos Cross-Over , Sucos de Frutas e Vegetais , Malus , Humanos , Ácido Ascórbico/urina , Ácido Ascórbico/administração & dosagem , Feminino , Adulto , Japão , Adulto Jovem , População do Leste Asiático
13.
Anal Chem ; 96(35): 14248-14256, 2024 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-39167046

RESUMO

Precise and rapid identification of pesticides is crucial to ensure a green environment, food safety, and human health. However, complex sample environments often hinder precise identification, especially for simultaneous differentiation of multiple pesticides. Herein, we first synthesize a Eu(III)-functionalized HOF-on-HOF composite (Eu@PFC-1@MA-TPA) and then utilize principal component analysis (PCA) and a machine learning (ML) algorithm to achieve simultaneous identification of the pesticides 2,6-dichloro-4-nitroaniline (DCN) and thiabendazole (TBZ) and their mixtures. Eu@PFC-1@MA-TPA displays high quantitative identification ability, which can distinguish single DCN and TBZ as low as 1 µM and their mixtures at 5 µM through PCA. In addition, the hydrogel film Eu@PFC-1@MA-TPA/AG is fabricated to monitor DCN and TBZ in drinking water, tap water, river water, and apple juice with high sensitivity. Furthermore, based on the obvious fluorescence color variance of pesticides, Eu@PFC-1@MA-TPA/AG achieves visual and in situ imaging detection of single DCN and TBZ and their mixtures. More importantly, we construct an intelligent artificial vision platform integrating Eu@PFC-1@MA-TPA/AG with a DenseNet algorithm, which can identify the concentrations and types of DCN and TBZ and their mixtures within 1 s with over 98% accuracy. This work develops a precise and rapid analysis method for simultaneous identification of multiple pesticides through combining a visualized fluorescence sensor and an ML algorithm.


Assuntos
Európio , Aprendizado de Máquina , Praguicidas , Praguicidas/análise , Európio/química , Tiabendazol/análise , Água Potável/análise , Poluentes Químicos da Água/análise , Sucos de Frutas e Vegetais/análise , Análise de Componente Principal , Fluoretos/química , Fluoretos/análise
14.
Sci Rep ; 14(1): 19971, 2024 08 28.
Artigo em Inglês | MEDLINE | ID: mdl-39198517

RESUMO

Acute myeloid leukemia (AML) is a hematologic neoplasm, characterized by a blockage of differentiation and an unconstrained proliferation of immature myeloid cells. Recently, the survival of leukemia patients has increased thanks to the use of differentiating agents, though these may cause serious side effects. Hence, the search for safer differentiating compounds is necessary. Our aim was to assess the pro-differentiating effects of a flavonoid-rich extract of bergamot juice (BJe) in human monocytic leukemia THP-1 cells, an in vitro AML model. For the first time, we showed that treatment with BJe induced differentiation of THP-1 cells, changes in cell morphology and increased expression of differentiation-associated surface antigens CD68, CD11b and CD14. Moreover, BJe enhanced protein levels of autophagy-associated markers, such as Beclin-1 and LC3, as well as induced the phosphorylation of the MAPKs JNK, ERK and p38, hence suggesting a potential mechanism underlying its antiproliferative effects. Indeed, parallel experiments highlighted that BJe was able to hamper THP-1 cell growth. In conclusion, our study suggests that BJe induces the differentiation of THP-1 cells and reduces their proliferation, highlighting its potential in differentiation therapy of AML.


Assuntos
Autofagia , Diferenciação Celular , Citrus , Flavonoides , Extratos Vegetais , Humanos , Citrus/química , Flavonoides/farmacologia , Diferenciação Celular/efeitos dos fármacos , Células THP-1 , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Autofagia/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sucos de Frutas e Vegetais/análise , Leucemia Mieloide Aguda/tratamento farmacológico , Leucemia Mieloide Aguda/patologia , Leucemia Mieloide Aguda/metabolismo
15.
Int J Mol Sci ; 25(16)2024 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-39201566

RESUMO

Fermenting fruit juices with lactic acid bacteria (LAB) is a sustainable method to enhance fruit harvests and extend shelf life. This study focused on blackberries, rich in antioxidants with proven health benefits. In this research, we examined the effects of fermentation (48 h at 37 °C) at 28 days on whey-supplemented (WH, 1:1) blackberry juice (BJ) inoculated with two LAB mixtures. Consortium 1 (BJWH/C1) included Levilactobacillus brevis, Lactiplantibacillus plantarum, and Pediococcus acidilactici, while consortium 2 (BJWH/C2) comprised Lacticaseibacillus casei and Lacticaseibacillus rhamnosus. All of the strains were previously isolated from aguamiel, pulque, and fermented milk. Throughout fermentation and storage, several parameters were evaluated, including pH, lactic acid production, viscosity, stability, reducing sugars, color, total phenolic content, anthocyanins, and antioxidant capacity. Both consortia showed a significant increase in LAB count (29-38%) after 16 h. Sample BJWH/C2 demonstrated the best kinetic characteristics, with high regression coefficients (R2 = 0.97), indicating a strong relationship between lactic acid, pH, and fermentation/storage time. Despite some fluctuations during storage, the minimum LAB count remained at 9.8 log CFU/mL, and lactic acid content increased by 95%, with good storage stability. Notably, sample BJWH/C2 increased the total phenolic content during storage. These findings suggest that adding whey enhances biomass and preserves physicochemical properties during storage.


Assuntos
Antioxidantes , Fermentação , Sucos de Frutas e Vegetais , Lactobacillales , Soro do Leite , Antioxidantes/metabolismo , Soro do Leite/metabolismo , Soro do Leite/química , Soro do Leite/microbiologia , Sucos de Frutas e Vegetais/microbiologia , Sucos de Frutas e Vegetais/análise , Lactobacillales/metabolismo , Rubus/química , Rubus/metabolismo , Rubus/microbiologia , Armazenamento de Alimentos/métodos , Concentração de Íons de Hidrogênio , Consórcios Microbianos/fisiologia , Ácido Láctico/metabolismo
16.
Nutrients ; 16(16)2024 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-39203773

RESUMO

Physical inactivity and poor dietary choices contribute to the rise in cardiometabolic diseases in the United States. It remains critical to identify strategies that may mitigate the negative impact of these behaviors. Several studies have shown that the consumption of dietary inorganic nitrate may improve vascular health and glucose regulation in animal models and some human studies. However, the improvements in glucose regulation have yet to be corroborated in humans with type 2 diabetes (T2D). Therefore, the purpose of this study was to assess the acute effects of beetroot juice (BRJ) on glycemic and hemodynamic responses in individuals with T2D while controlling for medication. Seven participants with a clinical diagnosis of T2D were recruited into this study and were temporarily removed from blood pressure- and glucose-lowering medications. Hemodynamic measurements (pulsewave velocity) and an oral glucose tolerance test (glycemic response) were measured following consumption of either BRJ or a denitrolized placebo. Saliva and blood samples were collected at baseline and two and four hours post supplementation to measure changes in nitrate and nitrite concentrations. We detected significant improvements in total plasma glucose exposure (p = 0.022) and the SVR change score (p = 0.009) in the BRJ condition. This study demonstrated that BRJ consumption can improve oral glucose tolerance in individuals with T2D while controlling for medication; however, future larger-cohort randomized controlled trials are needed to confirm if BRJ is a viable treatment for glucose control in individuals with T2D.


Assuntos
Beta vulgaris , Glicemia , Pressão Sanguínea , Diabetes Mellitus Tipo 2 , Sucos de Frutas e Vegetais , Nitratos , Humanos , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/dietoterapia , Diabetes Mellitus Tipo 2/sangue , Projetos Piloto , Beta vulgaris/química , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Masculino , Pressão Sanguínea/efeitos dos fármacos , Feminino , Pessoa de Meia-Idade , Nitritos/sangue , Teste de Tolerância a Glucose , Idoso , Método Duplo-Cego , Adulto
17.
Nutrients ; 16(16)2024 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-39203900

RESUMO

This systematic review and meta-analysis investigated the influence of dietary nitrate supplementation on performance metrics during cycling sprint exercise according to the PRISMA guidelines. Searches were conducted on MEDLINE, PubMed, ScienceDirect, Scopus, and SPORTDiscus databases up to September 2023. Inclusion criteria were healthy recreationally active men and women who consumed nitrate-rich and nitrate-deficient beetroot juice to assess performance outcomes of mean power, peak power, time-to-peak power, and minimum power during 30-s cycling sprints. Risk of bias was assessed using the Cochrane Risk of Bias 2 and TESTEX tools and funnel plots. A random effects model was performed on six studies and showed that dietary nitrate had significant effects on time-to-peak power (SMD: -0.66, 95% CI: -1.127 to -0.192, p = 0.006) but not on mean power, peak power, or minimum power. Subgroup analysis revealed that an acute low nitrate dose improved time-to-peak power (SMD: -0.977, 95% CI: -1.524 to -0.430, p < 0.001) but not after a multiday moderate nitrate dose (SMD: -0.177, 95% CI: -0.619 to -0.264, p = 0.431). These data suggest that acute nitrate supplementation can benefit time-to-peak power during 30-s cycling sprints, but due to the limited availability of data and heterogeneity in methodology, these results should be interpreted with caution. There was insufficient data on women to analyze sex-based differences. Future studies are required to provide insight on how supplementation regimen and population impact the effects of dietary nitrate for enhancing cycling sprint performance.


Assuntos
Desempenho Atlético , Ciclismo , Suplementos Nutricionais , Nitratos , Humanos , Nitratos/administração & dosagem , Desempenho Atlético/fisiologia , Ciclismo/fisiologia , Feminino , Masculino , Adulto , Beta vulgaris , Sucos de Frutas e Vegetais
18.
J Chromatogr A ; 1733: 465259, 2024 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-39178659

RESUMO

Aluminum-based metal organic framework composite containing ionic liquid was prepared and used as sorbent for extraction of sixteen polycyclic aromatic hydrocarbons in list of priority pollutants of United States Environmental Protection Agency before their analysis by gas chromatography/mass spectrometry. The dispersive micro solid-phase extraction method, known as a simple and fast method, was preferred as the extraction method. The optimized parameter conditions were 5 mL of sample solution, 10 min sonication by ultrasonic bath, 30 mg of sorbent, 30 °C extraction temperature, 0.1 mL of hexane as elution solvent with 5 min elution time. The suggested method presented that limit of detection and limit of quantification were in the range of 0.01-0.10 µg l-1, and 0.04-0.33 µg L-1, respectively. The intra-day and inter-day repeatability were within the ranges of 1.18-4.88 % and 1.02-5.06 %, respectively. The recoveries for polycyclic aromatic hydrocarbons in peach juice, cherry juice, tap water and rain water samples were obtained in the range of 84.9-99.9 % for spiked 5, 50 and 100 µg l-1 standard polycyclic aromatic hydrocarbons solution.


Assuntos
Sucos de Frutas e Vegetais , Cromatografia Gasosa-Espectrometria de Massas , Líquidos Iônicos , Limite de Detecção , Estruturas Metalorgânicas , Hidrocarbonetos Policíclicos Aromáticos , Poluentes Químicos da Água , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/isolamento & purificação , Líquidos Iônicos/química , Sucos de Frutas e Vegetais/análise , Estruturas Metalorgânicas/química , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Sonicação , Microextração em Fase Sólida/métodos , Reprodutibilidade dos Testes
19.
Food Chem ; 460(Pt 3): 140756, 2024 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-39121782

RESUMO

Vitamin D plays a crucial role in bone, immunology, and neurophysiological functions but has inadequate bioavailability in the human body. In this paper, six different liquid beverages were used for vitamin D3 fortification, investigating the effect of different food matrices on the bioaccessibility of vitamin D. Not from concentrate (NFC) apple juice (9.34%) and NFC orange juice (8.12%) presented about 20% higher bioaccessibility of vitamin D3 than soybean and skim milk, and achieved a similar value of whole milk (8.04%). Meanwhile, the bioaccessibility of NFC apple and orange juice was markedly about 120% higher than that of apple clear juice. From the correlation analysis, the bioaccessibility of VD3 indicated significant correlations with small intestine retention (0.82) and viscosity (0.66). But small intestinal particle size showed a negative effect on bioaccessibility (-0.78). Therefore, food components, delivery matrices, and physicochemical properties of digesta were key factors to achieve higher bioaccessibility for guiding formulation design.


Assuntos
Disponibilidade Biológica , Colecalciferol , Sucos de Frutas e Vegetais , Leite , Colecalciferol/análise , Colecalciferol/metabolismo , Colecalciferol/química , Animais , Leite/química , Leite/metabolismo , Sucos de Frutas e Vegetais/análise , Humanos , Malus/química , Malus/metabolismo , Alimentos Fortificados/análise , Bebidas/análise , Viscosidade , Tamanho da Partícula , Digestão
20.
Int J Biol Macromol ; 278(Pt 2): 134689, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39142475

RESUMO

Patulin (PAT) is a highly toxic mycotoxin, which can contaminate fruits and their products and cause harm to human health. Cellulose nanocrystals (CNCs) were functionalized by magnetite nanoparticles, dopamine (DA) and polyethyleneimine (PEI) to form a multifunctional nanocarrier (DA/PEI@Fe3O4/CNCs) for immobilizing aldo-keto reductase (MgAKR) to degrade PAT. The MgAKR-DA/PEI@Fe3O4/CNCs were reusable and environmentally friendly due to its surface area, high magnetization value, and oxygen/amine function. The immobilization method significantly improved reusability, resistance to proteolysis, temperature stability and storage stability of MgAKR-DA/PEI@Fe3O4/CNCs. With NADPH as a coenzyme, the detoxification rate of MgAKR-DA/PEI@Fe3O4/CNCs on PAT reached 100 % in phosphate buffer and 98 % in fresh pear juice. The quality of fresh pear juice was unaffected by MgAKR-DA/PEI@Fe3O4/CNCs and could be quickly separated by magnet after detoxification, which was convenient for recycling. It has broad application prospects in the control of PAT contamination in beverage products containing fruit and vegetable ingredients.


Assuntos
Aldo-Ceto Redutases , Celulose , Dopamina , Enzimas Imobilizadas , Sucos de Frutas e Vegetais , Patulina , Polietilenoimina , Pyrus , Celulose/química , Polietilenoimina/química , Pyrus/química , Enzimas Imobilizadas/química , Enzimas Imobilizadas/metabolismo , Patulina/química , Sucos de Frutas e Vegetais/análise , Dopamina/química , Aldo-Ceto Redutases/química , Aldo-Ceto Redutases/metabolismo , Nanopartículas de Magnetita/química , Nanopartículas/química
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