Evaluation of some garden flowers as specialty cut flowers in Eskisehir province-Türkiye.

Specialty cut flowers are in demand, especially in the domestic market as they can be grown with low production costs without the need for specially equipped greenhouses and offer diversity in terms of form, texture, and colour. These products, which are widely cultivated in the USA, are not well known in Türkiye. One of the main problems of the Turkish cut flower sector is its dependence on foreign inputs and the lack of product diversity. Therefore, specialty cut flower production can be an alternative crop for Türkiye, which has climatic advantages. The cut flower potential of plants such as Zinnia elegans, Tagates erecta, Helianthus annuus, Gomphrena globosa, Centaurea cyanus, and Cleome spinosa that are commonly grown in gardens has been evaluated. After harvesting these flowers grown in an open field in June-October 2020, the stem length (cm), stem thickness (mm), flower length (cm), flower diameter (cm), flower weight (g), and vase life (days) were measured. As a result of the evaluations, considering the phenological findings such as flowering and harvesting period, Zinnia elegans, Tagates erecta, Helianthus annuus, Gomphrena globosa, and Centaurea cyanus were found to be suitable for Eskisehir climatic conditions due to their long flowering periods. On the other hand, if the stem length value, which is one of the most important parameters for cut flowers, is taken as a reference, the minimum stem length value of 30 cm and above is met by Zinnia elegans, Tagates erecta, Helianthus annuus, and Cleome spinosa while the vase life value of 6 days and above is met by Zinnia elegans, Tagates erecta, Helianthus annuus, Gomphrena globosa, and Cleome spinosa. However, Cleome spinosa was not found to be suitable for the region due to its low yield value and short flowering period, while Zinnia elegans, Helianthus annuus, Tagates erecta, and Gomphrena globosa were found to be plants that could be evaluated for the region. In addition, it is believed that the cultivation of specialty cut flowers, with the selection of suitable species, will be an alternative production in regions without climatic advantages.

Antimicrobial potential of Streptomyces coeruleofuscus SCJ isolated from microbiologically unexplored garden soil in Northwest Morocco.

Research on microorganisms in various biotopes is required to identify new, natural potent molecules. These molecules are essential to control the development of multi-drug resistance (MDR). In the present study, a Streptomyces sp., namely SCJ, was isolated from a soil sample collected from a Moroccan garden. SCJ isolate was identified on the basis of a polyphasic approach, which included cultural, micro-morphological, biochemical, and physiological characteristics. The sequence of the 16S rRNA gene of the SCJ strain showed 99.78% similarity to strains of Streptomyces coeruleofuscus YR-T (KY753282.1). The preliminary screening indicated that the SCJ isolate exhibited activity against Candida albicans ATCC 60,193, Escherichia coli ATCC 25,922, Staphylococcus aureus CECT 976, Staphylococcus aureus ATCC 25,923, Bacillus cereus ATCC 14,579, Pseudomonas aeruginosa ATCC 27,853, as well as various other clinical MDR bacteria and five phytopathogenic fungi. The ethyl acetate extract of the isolated strain demonstrated highly significant (p < 0.05) antimicrobial activity against multi-resistant bacteria and phytopathogenic fungi. The absorption spectral analysis of the ethyl acetate extract of the SCJ isolate obtained showed no absorption peaks characteristic of polyene molecules. Moreover, no hemolytic activity against erythrocytes was observed in this extract. GC-MS analysis of the ethyl acetate extract of the SCJ isolate revealed the presence of 9 volatile compounds including 3,5-Dimethylpyrazole, and pyrrolizidine derivatives (Pyrrolo[1,2-a]pyrazine 1,4-dione, hexahydro-3-(2-methylpropyl)), which could potentially explain the antimicrobial activity demonstrated in this study.

Human health and environmental risk assessment of metals in community gardens of Winnipeg, Manitoba, Canada.

Fresh produce is an important component of maintaining cognitive and physical health, particularly for children. A mechanism to increase access to fresh produce is the construction of community gardens in urban centres. While reducing barriers to nutritious food, the soil of the community garden can contain contaminants (e.g. metals) depending on the location and how the garden was constructed. This study quantified, for the first time, seven metals (As, Cd, Cr, Cu, Pb, Mn, and Ni) in soil from 83 community gardens across the City of Winnipeg in Manitoba, Canada. Concentrations of metals in soil were used to create distributions for environmental exposure and estimated daily intake, which were then used to determine exceedances of soil quality guidelines and acceptable daily intakes, respectively. Raised garden beds and gardens further from roads had typically lower concentrations of metals in surface gardens and those nearer to roads. While some concentrations of metals exceeded CCME guidelines levels for the protection of environmental health, the vast majority represent a low risk. For human health, only As posed a quantifiable risk of exceeding the USEPA acceptable daily intake via the consumption of produce from gardens, though this was < 1.2% for the whole population and < 10.2% for children aged 1 to 2 years. Overall, this study is the first to show that the concentration of the metals in soil from gardens typically poses a low risk to environmental and human health. We recommend the use of raised gardens to further mitigate risk.

Dynamic spatiotemporal changes of per- and polyfluoroalkyl substances (PFAS) in soil and eggs of private gardens at different distances from a fluorochemical plant.

Homegrown food serves as an important human exposure source of per- and polyfluoroalkyl substances (PFAS), yet little is known about their spatiotemporal distribution within and among private gardens. This knowledge is essential for more accurate site-specific risk assessment, identification of new sources and evaluating the effectiveness of regulations. The present study evaluated spatiotemporal changes of legacy and emerging PFAS in surface soil from vegetable gardens (N = 78) and chicken enclosures (N = 102), as well as in homegrown eggs (N = 134) of private gardens, across the Province of Antwerp (Belgium). Hereby, the potential influence of the wind orientation and distance towards a major fluorochemical plant was examined. The ∑short-chain PFAS and precursor concentrations were higher in vegetable garden soil (8.68 ng/g dry weight (dw)) compared to chicken enclosure soil (4.43 ng/g dw) and homegrown eggs (0.77 ng/g wet weight (ww)), while long-chain sulfonates and C11-14 carboxylates showed the opposite trend. Short-term (2018/2019-2022) changes were mostly absent in vegetable garden soil, while changes in chicken enclosure soils oriented S-SW nearby (<4 km) the fluorochemical plant were characterized by a local, high-concentration plume. Moreover, soil from chicken enclosures oriented SE and remotely from the plant site was characterized by a widespread, diffuse but relatively low-concentration plume. Long-term data (2010-2022) suggest that phaseout and regulatory measures have been effective, as PFOS concentrations nearby the fluorochemical plant in soil and eggs have declined from 25.8 to 2.86 ng/g dw and from 528 to 39.4 ng/g ww, respectively. However, PFOS and PFOA concentrations have remained largely stable within this timeframe in gardens remotely from the plant site, warranting further rapid regulation and remediation measures. Future monitoring efforts are needed to allow long-term comparison for multiple PFAS and better distinction from potential confounding variables, such as variable emission outputs and variability in wind patterns.

Production of Escovopsis conidia and the potential use of this parasitic fungus as a biological control agent of leaf-cutting ant fungus gardens.

This study was carried out to investigate the use of different substrates for the production of Escovopsis conidia and verify the virulence of four different isolates cultured on four types of substrates using a novel bioassay. Escovopsis isolates were molecularly identified, based on Internal Transcribed Spacer (ITS) nucleotide sequences. To evaluate conidial production, suspensions (1 × 106 conidia mL-1) of each Escovopsis isolate were inoculated onto four substrates (parboiled rice, white rice, rolled oats, and corn grits). After 14 days, conidial yields were assessed. The virulence of each isolate cultured on the four substrates was tested against Leucoagaricus fungus garden fragments, by directly applying 500 µL of each conidial suspension (1 × 107 conidia mL-1), and the development of the parasite was monitored daily until it completely colonized the fungus garden. It was observed that rolled oats were the best substrate for conidial production, with a yield of 1.7 × 107 to 2.0 × 108 conidia mL-1. Furthermore, isolate AT-01 produced the highest number of conidia when compared with the other isolates. Regardless of the substrate used to produce AT-01 conidia, this isolate completely colonized the fungus garden 6 days post inoculation (dpi), followed by AT-02, AC-01, and AC-2. High levels of both conidial production and virulence against the leaf-cutting ant fungus garden were observed here.

Light Intensity Modulates the Functional Composition of Leaf Metabolite Groups and Phyllosphere Prokaryotic Community in Garden Lettuce (Lactuca sativa L.) Plants at the Vegetative Stage.

Light intensity primarily drives plant growth and morphogenesis, whereas the ecological impact of light intensity on the phyllosphere (leaf surface and endosphere) microbiome is poorly understood. In this study, garden lettuce (Lactuca sativa L.) plants were grown under low, medium, and high light intensities. High light intensity remarkably induced the leaf contents of soluble proteins and chlorophylls, whereas it reduced the contents of leaf nitrate. In comparison, medium light intensity exhibited the highest contents of soluble sugar, cellulose, and free amino acids. Meanwhile, light intensity resulted in significant changes in the composition of functional genes but not in the taxonomic compositions of the prokaryotic community (bacteria and archaea) in the phyllosphere. Notably, garden lettuce plants under high light intensity treatment harbored more sulfur-cycling mdh and carbon-cycling glyA genes than under low light intensity, both of which were among the 20 most abundant prokaryotic genes in the leaf phyllosphere. Furthermore, the correlations between prokaryotic functional genes and lettuce leaf metabolite groups were examined to disclose their interactions under varying light intensities. The relative abundance of the mdh gene was positively correlated with leaf total chlorophyll content but negatively correlated with leaf nitrate content. In comparison, the relative abundance of the glyA gene was positively correlated with leaf total chlorophyll and carotenoids. Overall, this study revealed that the functional composition of the phyllosphere prokaryotic community and leaf metabolite groups were tightly linked in response to changing light intensities. These findings provided novel insights into the interactions between plants and prokaryotic microbes in indoor farming systems, which will help optimize environmental management in indoor farms and harness beneficial plant-microbe relationships for crop production.

Is there any difference between the owners and the public in their visual impact assessments?--A case study of the front garden of multi-storey residential buildings.

As a special garden unique to China, the front garden of multi-storey residential buildings has certain public attributes but is managed by a single owner. In this study, the front gardens of multi-storey residential buildings in Jiangsu province, China, were set as research object. Meanwhile, the size of garden, plant type, plant vertical structure, color number, plant coverage, and fence material were chosen as the landscape features to be explored. Then the experiments were conducted to reveal the visual impact assessment rendered by the public with different demographic attributes and the results obtained were analyzed. As is indicated by the statistical analysis, significant differences exist between the owners and the public in their visual impact assessments of the front gardens; the six landscape features are the main factors that influence the public's visual impact assessment; and the public with different demographic attributes would render different visual impact assessments of front gardens. This study offers valuable help for the design of front gardens of multi-storey residential buildings.

Pollutant accumulation and microbial community evolution in rain gardens with different drainage types at field scale.

Rain gardens play a key role in urban non-point source pollution control. The drainage type affects the infiltration processes of runoff pollutants. The soil properties and microbial community structures were studied to reveal the stability of the ecosystem in rain gardens with different drainage types under long-term operation. The results showed that the soil water content and total organic carbon in the drained rain gardens were always higher than that of the infiltrated ones. With the increase in running time, the contents of heavy metals in rain gardens showed significant accumulation phenomena, especially the contents of Zn and Pb in drained rain gardens were higher than that in infiltrated ones. The accumulation of pollutants resulted in lower microbial diversity in drained rain gardens than in infiltrated rain gardens, but the microbial community structures were the same in all rain gardens. The effects of drainage type on microbial community evolution were not significant, only the accumulation of heavy metals led to changes in the abundance of dominant microorganisms. There were differences in the soil environment of rain gardens with different drainage types. The long-term operation of rain gardens led to fluctuations in the soil ecosystem, while the internal micro-ecosystems of the drained rain gardens were in unstable states.

Aerosolomics based approach to discover source molecular markers: A case study for discriminating residential wood heating vs garden green waste burning emission sources.

Biomass burning is a significant source of particulate matter (PM) in ambient air and its accurate source apportionment is a major concern for air quality. The discrimination between residential wood heating (RWH) and garden green waste burning (GWB) particulate matter (PM) is rarely achieved. The objective of this work was to evaluate the potential of non-targeted screening (NTS) analyses using HRMS (high resolution mass spectrometry) data to reveal discriminating potential molecular markers of both sources. Two residential wood combustion appliances (wood log stove and fireplace) were tested under different output conditions and wood moisture content. GWB experiments were carried out using two burning materials (fallen leaves and hedge trimming). PM samples were characterized using NTS approaches with both LC- and GC-HRMS (liquid and gas chromatography-HRMS). The analytical procedures were optimized to detect as many species as possible. Chemical fingerprints obtained were compared combining several multivariate statistical analyses (PCA, HCA and PLS-DA). Results showed a strong impact of the fuel nature and the combustion quality on the chemical fingerprints. 31 and 4 possible markers were discovered as characteristic of GWB and RWH, respectively. Complementary work was attempted to identify potential molecular formulas of the different potential marker candidates. The combination of HRMS NTS chemical characterization with multivariate statistical analyses shows promise for uncovering organic aerosol fingerprinting and discovering potential PM source markers.

Genetic parameter estimates for the use of an aviary with winter garden by laying hens.

The behavioral activity of laying hens in an aviary is indicative of their welfare and health. Furthermore, hens' usage of the different locations within an aviary has been shown to influence laying performance and egg quality. For example, hens that spent a longer duration of time in the nest during laying were observed to have lower laying performance. Therefore, understanding genetics of laying hens' usage of the aviary could be important for predicting egg quality, production traits and health and welfare. The objectives of this study were to estimate genetic parameters for duration of time spent at different locations within the aviary and an adjacent winter garden using a multivariate repeatability model and to compare correlations between time spent in these locations. For this study, a total of 1,106 Dekalb white laying hens (Hendrix Genetics) were genotyped using a proprietary 60K SNP array. These hens had access to 5 different zones within the aviary, which included the top level tier, nest box tier, lower level tier, floor littered area and a winter garden. Hens were in the aviary for a total of 290 d and daily records of duration were collected for each hen visit to any location in the aviary, culminating in a total of 937,740 records. Heritability estimates ranged from 0.05 (0.01) to 0.28 (0.03) for the duration of time spent in the different zones. The lowest heritability was estimated for time spent at the lower level tier, while a higher heritability was estimated for time spent in the floor littered area. A moderately high negative genetic correlation of -0.59 (0.08) was observed between time spent in the top level tier and time spent in the floor littered area, while a favorable correlation of 0.37 (0.14) was found between time spent in the lower level tier and time spent in the winter garden. The findings of this study show that the duration of time spent at different zones within an aviary has genetic basis and could be used for selecting animals for better performance and higher welfare.

Multiple potentially toxic elements in urban gardens from a Brazilian industrialized city.

Urban agriculture should be promoted as long as the food produced is safe for consumption. Located in the metropolitan region of São Paulo-Brazil, Santo André has intense industrial activities and more recently an increasing stimulus to urban gardening. One of the potential risks associated to this activity is the presence of potentially toxic elements (PTEs). In this study, the concentration of PTEs (As, Ba, Cd, Co, Cu, Cr, Ni, Mo, Pb, Sb, Se, V and Zn) was evaluated by soil (n = 85) and soil amendments (n = 19) in urban gardens from this municipality. Only barium was above regulatory limits in agricultural soil ranging from 20 to 112 mg kg-1. Geochemical indexes (Igeo, Cf and Er) revealed moderate to severe pollution for As, Ba, Cr, Cu, Pb Se and Zn, especialy in Capuava petrochemical complex gardens. A multivariate statistical approach discriminated Capuava gardens from the others and correlated As, Cr and V as main factors of pollution. However, carcinogenic and non-carcinogenic risks were below the acceptable range for regulatory purposes of 10-6-10-4 for adults. Soil amendments were identified as a possible source of contamination for Ba, Zn and Pb which ranged from 37 to 4137 mg kg-1, 20 to 701 mg kg-1 and 0.7 to 73 mg kg-1, respectively. The results also indicated the presence of six pathogenic bacteria in these amendments. Besides that, the occurrence of antimicrobial resistance for Shigella, Enterobacter and Citrobacter isolates suggests that soil management practices improvement is necessary.

Relationship between home garden ownership and the consumption of fruits and vegetables.

OBJECTIVE: This study assessed the extent to which access to home gardens associate with the frequency of fruit and vegetable (FV) consumption. SETTING: The study was carried out in fifty rural communities in Northern Ghana where food insecurity and malnutrition including micronutrient deficiencies are prevalent. DESIGN: A community-based comparative analytical cross-sectional study. PARTICIPANTS: A sample of 847 randomly selected rural households. RESULTS: The proportion of households that consumed FV at least 3 d in a week was 45 %. Members in households who owned a home garden were 1·5 times more likely to consume FV at least 3 d in a week (adjusted OR (AOR) = 1·46 (95 % CI 1·06-2·0)), compared with their counterparts who had no home gardens. Furthermore, households in which mothers had a positive attitude towards FV consumption were 1·6 times more likely to consume FV (AOR = 1·63 (95 % CI 1·17-2·27)) compared with mothers who were less positive. CONCLUSIONS: Our results suggest that food and nutrition policy measures that promote home gardens can improve consumption of diversified diets including FV among vulnerable rural households in Northern Ghana. Additionally, households with lower income may benefit from nutrition behaviour change communication campaigns directed towards increasing a positive attitude to FV intake.

Tardiphaga alba sp. nov., a heavy-metal-tolerant bacterium isolated from garden soil.

A previously undescribed, heavy-metal-tolerant, motile, Gram-negative bacterium, designated strain SK50-23T, was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SK50-23T was closely related to Tardiphaga robiniae LMG 26467T and the non-phototrophic 'Rhodopseudomonas boonkerdii' NS23T (98.1 and 97.3 % 16S rRNA gene sequence similarity, respectively). Strain SK50-23T possessed a circular genome of 5.86 Mb, with a DNA G+C content of 61.9 mol%. Digital DNA-DNA hybridization showed 20.8-21.6 % similarity between strain SK50-23T and related species. In addition, the whole-genome average nucleotide identity values between strain SK50-23T and related species ranged from 75.1 to 83.5 %. The major cellular fatty acid identified in strain SK50-23T was C18 : 1ω7c, and the main isoprenoid quinone present was ubiquinone Q-10. Strain SK50-23T could be assigned to the genus Tardiphaga with the species name Tardiphaga alba sp. nov. based on morphological, chemotaxonomic and genome-based taxonomic characteristics, and 16S rRNA gene-based phylogenetic characteristics. The type strain of the proposed novel species is SK50-23T (=NBRC 108825T=CGMCC No. 1.12037T).

Open Data governance at the Canadian Open Neuroscience Platform (CONP): From the Walled Garden to the Arboretum.

Scientific research communities pursue dual imperatives in implementing strategies to share their data. These communities attempt to maximize the accessibility of biomedical data for downstream research use, in furtherance of open science objectives. Simultaneously, such communities safeguard the interests of research participants through data stewardship measures and the integration of suitable risk disclosures to the informed consent process. The Canadian Open Neuroscience Platform (CONP) convened an Ethics and Governance Committee composed of experts in bioethics, neuroethics, and law to develop holistic policy tools, organizational approaches, and technological supports to align the open governance of data with ethical and legal norms. The CONP has adopted novel platform governance methods that favor full data openness, legitimated through the use of robust deidentification processes and informed consent practices. The experience of the CONP is articulated as a potential template for other open science efforts to further build upon. This experience highlights informed consent guidance, deidentification practices, ethicolegal metadata, platform-level norms, and commercialization and publication policies as the principal pillars of a practicable approach to the governance of open data. The governance approach adopted by the CONP stands as a viable model for the broader neuroscience and open science communities to adopt for sharing data in full open access.

Smellscape as a healing factor in institutional gardens to enhance health and well-being for older people with dementia: A scoping review.

BACKGROUND: There is mounting evidence for the health benefits of aromatic scents for the older people with dementia. However, existing research has focused on indoor aromatherapy using essential oils. It is necessary to explore the health benefits of smellscapes in the outdoor environment for older people with dementia. AIMS AND OBJECTIVES: This scoping review aims to examine existing evidence for smellscape as a healing factor in institutional garden for older people with dementia, try to bridge the knowledge gaps between outdoor sensory garden scents and aromatherapy to develop green care techniques that incorporate outdoor activities. METHODS: Seven databases (Scopus, PubMed, PsycINFO, CINAHL, MEDLINE, Embase and Web of Science) were searched with English language articles published between 1990 and 2022. The PRISMA-ScR Checklist was used. RESULTS: Out of 1013 articles, 11 meet the inclusion criteria. The comprehensive health outcomes include five aspects: mental health; physical health; reduced agitation behaviour; improved cognitive function; and well-being. These aspects are part of the rehabilitation model comprising the person (older people with dementia), environment (garden smellscapes) and outdoor activities (active or passive interventions or a combination). CONCLUSIONS: The smellscape, as a healing factor in the garden, not only benefits from evidence on indoor aromatherapy but also creates a sensory environment for older people with dementia by compensating for functional impairment, activity support and environmental creation, thereby promoting enhanced health and well-being. RELEVANCE TO CLINICAL PRACTICE: The research on the healing effects of smellscapes presented in this review offers a novel environmental intervention technique for transferring evidence on essential oils to outdoor sensory gardens. This green care technique is suggested to assist in the creation of healing environments and interventions for people with dementia who cannot be cured. NO PATIENT OR PUBLIC CONTRIBUTION: This scoping review did not directly involve patient or public contributions to the manuscript.

Neighborhood greenness and participation in specific types of recreational physical activities in the Sister Study.

BACKGROUND: Urban green spaces have been consistently shown to have important human health benefits across a range of outcomes. These benefits are thought to be achieved, in part, because urban greenness provides opportunities for participation in recreational activity. However, the findings from studies that have assessed links between exposure to greenness and physical activity have been mixed. To date, few studies have examined association between greenness and specific types of recreational physical activities. OBJECTIVE: We evaluated associations between measures of greenness and specific types of recreational physical activities. Moreover, we explored the extent to which these associations were modified by socioeconomic conditions, and regionally. METHODS: We analyzed cross-sectional data from 49,649 women in the Sister Study and assigned three residentially-based measures of greenness based on national land cover data at buffer distances of 250 m and 500 m. Data on participation in up to ten specific recreational physical activities, including time spent in each activity were collected. Logistic regression was used to estimate odds ratios (OR) and their 95% confidence intervals (CI) controlling for confounders. RESULTS: Compared to those in the lowest tertile of greenness, participants in the upper tertile of greenness within a 500 m buffer, were more likely to garden (OR = 1.46, 95% CI = 1.25,1.69), participate in sports (OR = 1.28, 95% CI = 1.19,1.38), run (OR = 1.15, 95% CI = 1.04,1.27), walk (OR = 1.11, 95% CI = 1.06,1.16), and engage in conditioning exercises (OR = 1.10, 95% CI = 1.05,1.16) at least once a week for at least one month over the past year. These associations were modified by household income and US region. DISCUSSION: Our findings suggest a beneficial effect of greenness on physical activity and provide additional information to inform planning of green environments that contribute to better health and wellbeing.

Green Roof Gardens - Selecting Allergy-Friendly Vegetation: A Global Allergy and Asthma Excellence Network (GA²LEN) Position Paper.

Green roof gardens are important for planetary health by mitigating the effects of urbanization. Because of the nature of green roof gardens, only particular plants can be used. The allergologic impact of these plants remains ill-characterized and guidance on building allergy-friendly green roof gardens is missing. To address this gap, we investigated the plant spectrum of several German green roof companies and categorized plants based on their primary pollination mechanism. Except for grasses, most plants were insect-pollinated and of low allergenicity. In addition, we conducted a review on the allergologic impact of plants used for green roof gardens. Our aim was to provide landscape architects with guidance on how to develop allergy-friendly green roof gardens. We highlight the need for universally accepted standards for assessing the allergenicity of roof top plants. Also, we recommend the joint development, by green roof producers and allergists, of criteria for allergy-friendly roof gardens. Their implementation may help to reduce the risk of allergen sensitization and allergy exacerbation, such as by avoiding the use of wind-pollinated plants of proven allergenicity including grasses. Green infrastructure, such as green roofs, should benefit planetary health without increasing the prevalence and burden of allergies.

A charred body inside a burning car with a garden hose connected to the exhaust pipe: Post-mortem self-immolation, a complex or a complicated suicide after the ingestion of alcohol? The importance of an interdisciplinary approach.

The analysis of charred bodies represents a serious challenge for forensic pathologists, and an interdisciplinary approach is often the only way to determine the cause and manner of death. We present an unusual case in which the charred body of a 61-year-old man was found inside his burning vehicle. In order to determine cause and manner of death, an interdisciplinary team was employed, with experts in forensic pathology, forensic radiology, toxicology and fire investigations. Post-mortem computed tomography, autopsy and toxicology ruled out the presence of trauma injury and detected signs of vital exposure to fire and blood alcohol levels. On the other hand, according to fire investigations, the fire started inside the car and partially burned fragments of a garden hose were found along the right side of the car. A suicide could therefore be hypothesized, with the man having attempted to poison himself with the car's exhaust fumes and having set the car on fire. The death was consistent with a complicated suicide in which the victim, in a state of reduced capability, accidentally set his car on fire and was unable to escape. The hypothesis of a complex suicide, with the car having been set deliberately on fire, could not, however, be ruled out.