In vivo studies of amino acid, water and electrolyte transport in the small intestine of normal and magnesium depleted rats

Net amino acid, water and electrolyte transport from the small intestine in vivo was studied in normal and magnesium depleted rats, by a single pass perfusion technique. The net absorption of the amino acids, alanine and lysine increased with increasing concentration of the amino acid in the perfusion solution, within the concentration range studied (10 and 50mM). In all cases alanine was absorbed faster than lysine. Dietary magnesium depletion, lasting for a period of twenty eight days did not affect the net rate of transport of these two amino acids in any of the regions of the small intestine which were studied. In general, the presence of amino acids increased the absorption of water by comparison with that from saline. However, lysine at a concentration of 50mM tended to inhibit water absorption by comparison with that observed in the presence of alanine at 50mM. Magnesium depletion did not in general affect net transport rates of water in the presence and absence of amino acids. However, when alanine at a concentration of 50mM was perfused, water absorption was inhibited in the magnesium depleted rats. The presence of amino acids did not affect the transport of sodium in any of the regions of the small intestine. However, magnesium depletion did severely inhibit sodium transport, especially in the presence of alanine at a concentration of 50mM in all three regions of the small intestine. Amino acids stimulated the absorption of chloride by all segments of the small intestine by comparison with that from saline alone. Magnesium depletion however, significantly reduced chloride ion absorption by all three segments. Good correlations were found between the transport rates of sodium and chloride, sodium and water, and total solute and water in all three regions of the small intestine (AU)

Action of antihistamine drugs in vitro-II. ion movements and phosphoproteins in whole cells

Slices of rat liver and suspensions of ascites cells were used to determine the effects of antihistamine drugs upon ion movements; it was shown that these drugs inhibit uptake of potassium in such systems. The relationship between phosphoproteins and these effects was also investigated, and there appeared to be a direct relationship between protein phosphorylation and water and ion movements; this relationship is not mediated by effects on ATP levels or turnover rates. The nature of the protein fraction that takes up 32P and shows diminished turnover in the presence of the drugs has been established by partial hydrolysis and identification of radioactive phosphoserine by chromatography on ion-exchange columns and upon paper(AU)