RESUMO
A recombinant protein of the human T cell lymphotropic virus type I (HTLV-I) gp46 outer membrane envelope, MTA-4 (residues 129-203), reacted by Western blot with sera from HTLV-I-infected individuals from the United States and Jamaica but not with 24 (10 percent) of 242 Japanese sera. A related gp46 recombinant protein, MTA-1 (residues 162-209), reacted with all 58 sera from HTLV-I-infected US and Jamaican individuals and 238 of 242 sera from infected Japanese (combined sensitivity of 99 percent). Neither recombinant showed reactivity to sera from HTLV-II-infected individuals or uninfected controls. The reactivity of recombinant proteins containing the region of HTLV-II gp46 analogous to MTA-1 was also evaluated by Western blot: GH2-K15 (residues 157-205) and GH2-K55 (residues 162-205) reacted with 88 (98 percent) and 89 (99 percent), respectively, of 90 sera from HTLV-II-infected individuals but not with sera from HTLV-I-infected individuals or uninfected controls. These recombinant proteins should permit the development of assays to unambiguously confirm and differentiate HTLV-I and HTLV-II infections. (AU)
Assuntos
Humanos , Anticorpos Antideltaretrovirus/biossíntese , Antígenos HTLV-I/imunologia , Infecções por HTLV-I/diagnóstico , Antígenos HTLV-II/imunologia , Infecções por HTLV-II/diagnóstico , Sequência de Aminoácidos , Anticorpos Monoclonais/diagnóstico , Epitopos/imunologia , Western Blotting , Diagnóstico Diferencial , Antígenos HTLV-I , Antígenos HTLV-II , Jamaica , Japão , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Proteínas Recombinantes/imunologia , Estados UnidosAssuntos
21003 , Anticorpos Antivirais/análise , Vírus Bluetongue/imunologia , Bovinos/microbiologia , Reoviridae/imunologia , Anticorpos Antivirais/imunologia , Especificidade de Anticorpos , Epitopos , Vírus Bluetongue/classificação , Búfalos/microbiologia , América Central/epidemiologia , Estudos Prospectivos , Distribuição Aleatória , Sorotipagem/veterinária , Ovinos/microbiologia , Fatores de Tempo , JamaicaRESUMO
Monoclonal antibodies specific for human globin chains have been prepared and the following strategy has been applied in delimiting the antigenic sites involved in antibody binding. The structural sites of the human globin subunit that might be recognised by the monoclonal antibody were deduced from comparisons of the primary structures of mamalian globin chains that did or did not react with the antibody. The involvement of individual residues at these specific sites was subsequently tested by reacting the antibody with abnormal human haemoglobins in which there was either a substitution or a structural site recognised by monoclonal antibody HuHb á 3-2 (an antibody that reacts with the adult haemoglobins from man and macaque monkey, but not with those from baboon and mouse) includes the aspartic acid residue at position 52 of the á-globin subunit.(AU)
