Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma: An in vitro study
Med. oral patol. oral cir. bucal (Internet)
; 22(3): e289-e296, mayo 2017. ilus, tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-163195
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
BACKGROUND:
Hyperthermia is considered an efficient complement in the treatment of head and neck squamous cell carcinoma (HNSCC). Hyperthermia induces cell apoptosis in a temperature- and time-dependent manner. However, the molecular mechanism of hyperthermia remains unclear. The aim of this study was to investigate the mechanism of apoptosis induced by ultrasound hyperthermia in HNSCC cell lines HN-30 and HN-13. MATERIAL ANDMETHODS:
We examined the dynamic changes of early apoptosis and secondary necrosis in HN-30 and HN-13 cells treated by hyperthermia at 42°C for 10 min. We further examined mitochondrial membrane potential in vitro by ultrasound hyperthermia for different heating temperatures (38-44°C, 10 min) and heating times (42°C, 10-50 min). After heating by ultrasound at 42°C for 10 min, the apoptosis index achieved its highest level at 8 h after treatment, decreased rapidly and remained constant at a reduced level at 12 h.RESULTS:
The level of secondary necrosis increased with the level of early apoptosis but remained at a higher level until 14 h. The level of secondary necrosis correlated with the level of early apoptosis (HN-13 r = 0.7523, P = 0.0030; HN-30 r = 0.6510, P = 0.016). The fractions of cells with low mitochondrial membrane potential (Δψ) in the heating-temperature grads group and heating-time grads group decreased significantly over time. Therefore, HN-30 and HN-13 cells developed apoptosis after ultrasound hyperthermia treatment with decreases in the mitochondrial transmembrane potential level.CONCLUSIONS:
Ultrasound hyperthermia induces apoptosis in HN-30 and HN-13 cells, possibly via the mitochondrial caspase pathway
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Carcinoma de Células Escamosas
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Neoplasias de Cabeça e Pescoço
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Hipertermia Induzida
Limite:
Humanos
Idioma:
Inglês
Revista:
Med. oral patol. oral cir. bucal (Internet)
Ano de publicação:
2017
Tipo de documento:
Artigo
Instituição/País de afiliação:
Central Research Institute of Dentistry and Maxillofacial Surgery/Russia
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Ninth Peoples Hospital/China
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The University of Texas MD Anderson Cancer Center/United States