Efficient Generation of an Fah/Rag2 Dual-Gene Knockout Porcine Cell Line Using CRISPR/Cas9 and Adenovirus.

Gao, Mengyu; Zhang, Bingqi; He, Yuting; Yang, Qing; Deng, Lihong; Zhu, Yuqi; Lai, Enjiang; Wang, Menghua; Wang, Laduona; Yang, Guang; Liao, Guangneng; Bao, Ji; Bu, Hong

Gao, Mengyu; Zhang, Bingqi; He, Yuting; Yang, Qing; Deng, Lihong; Zhu, Yuqi; Lai, Enjiang; Wang, Menghua; Wang, Laduona; Yang, Guang; Liao, Guangneng; Bao, Ji; Bu, Hong.

Afiliação

Gao M; 1 Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.
Zhang B; 1 Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.
He Y; 1 Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.
Yang Q; 1 Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.
Deng L; 2 Laboratory of Pathology, West China School of Medicine, Sichuan University, Chengdu, China.
Zhu Y; 2 Laboratory of Pathology, West China School of Medicine, Sichuan University, Chengdu, China.
Lai E; 2 Laboratory of Pathology, West China School of Medicine, Sichuan University, Chengdu, China.
Wang M; 2 Laboratory of Pathology, West China School of Medicine, Sichuan University, Chengdu, China.
Wang L; 2 Laboratory of Pathology, West China School of Medicine, Sichuan University, Chengdu, China.
Yang G; 3 Department of Experimental Animal Center, West China Hospital, Sichuan University, Chengdu, China.
Liao G; 3 Department of Experimental Animal Center, West China Hospital, Sichuan University, Chengdu, China.
Bao J; 1 Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.
Bu H; 1 Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, NHFPC, West China Hospital, Sichuan University, Chengdu, China.

DNA Cell Biol ; 38(4): 314-321, 2019 Apr.

Article em En | MEDLINE | ID: mdl-30762444

Assuntos

Adenoviridae/genética; Sistemas CRISPR-Cas/genética; Proteínas de Ligação a DNA/deficiência; Proteínas de Ligação a DNA/genética; Técnicas de Inativação de Genes/métodos; Hidrolases/deficiência; Hidrolases/genética; Animais; Sequência de Bases; Linhagem Celular; Mutação; Suínos; Fatores de Tempo

Palavras-chave

CRISPR/Cas9; cell line; knockout; porcine

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adenoviridae / Proteínas de Ligação a DNA / Técnicas de Inativação de Genes / Sistemas CRISPR-Cas / Hidrolases Limite: Animals Idioma: En Revista: DNA Cell Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

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