Protective effects of fruit-juices on sperm viability of West African Dwarf goat bucks during cryopreservation
Anim. Reprod. (Online)
; 13(1): 7-13, jan.mar. 2016. tab
Artigo
em Inglês
| VETINDEX
| ID: biblio-1461194
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
The present study was conducted to determine the effects of adding fruit-juices to semen extenders on viability of buck spermatozoa during cryopreservation in two studies. Pooled semen samples obtained from West African Dwarf (WAD) goat bucks were diluted with Tris-egg yolk based extenders. In study I, the diluted semen samples were supplemented with orange (Citrus sinensis), cucumber (Cucumis sativus) and pineapple (Ananas comosus) juices (2.5, 5, 7.5 and 10 ml/100 ml). In study II, the diluted semen samples were supplemented with 5 ml/100 ml of different combinations of fruitjuices consisting of pineapple and orange juices (TEYPO) at a 11 ratio, pineapple and cucumber juices (TEYPC) at a 11 ratio, orange and cucumber juices (TEYOC) at a 11 ratio, and pineapple, orange and cucumber juices (TEYPOC) at a 111 ratio, respectively. The diluted semen samples were cryopreserved and thereafter evaluated for sperm viability parameters. The extenders supplemented with 7.5 and 10% orange, and 10% pineapple had higher (P < 0.05) motility compared to the control. There was higher (P < 0.05) acrosome integrity in extenders supplemented with orange and pineapple at all levels compared to the control while cucumber at 10% had higher (P < 0.05) acrosome integrity compared to other levels and the control. The extenders supplemented with cucumber, orange and pineapple had higher (P < 0.05) membrane integrity compared to the control except at 7.5% of extender supplemented with cucumber. Similarly, the extenders supplemented with cucumber, orange and pineapple had lower (P < 0.05) sperm abnormality compared to the control. The extenders supplemented with cucumber, orange and pineapple at 2.5, 10 and 7.5% respectively had lower (P < 0.05) concentrations of MDA compared to other levels and the control...
Texto completo:
Disponível
Base de dados:
VETINDEX
Assunto principal:
Preservação do Sêmen
/
Criopreservação
/
Sucos de Frutas e Vegetais
Idioma:
Inglês
Revista:
Anim. Reprod.
/
Anim. Reprod. (Online)
Ano de publicação:
2016
Tipo de documento:
Artigo
/
Documento de projeto
Instituição/País de afiliação:
Federal University of Agriculture Abeokuta/NG
/
Federal University of Agriculture Abeokuta/Nigéria