Vocalizations of the Pekin duck (Anas platyrhynchos domesticus): how stimuli, sex, and social groups affect their vocal repertoire.

Pekin ducks are exposed to stressors such as heat stress, enteric pathogens, mycotoxins, and other environmental stressors. We know from wild bird literature that birds communicate through vocalizations. We hypothesized that Pekin ducks would have a diverse repertoire that is affected by the sex, social group, and specific stimuli. We utilized adult Pekin ducks to develop a vocal repertoire. We placed 1 to 4 ducks of varying sexes into a sound chamber with various stimuli used to encourage new vocalizations. Birds were recorded for 20 min with several variations of number and sexes of ducks. Once the ducks were recorded each vocalization that was clipped was named based on a predetermined naming system. We characterized the vocal system of the ducks under each stimulus and social treatment in 4 ways: overall call rates, call diversity, call repertoire, and call spectral properties. In all cases, normality of residuals and homogeneity of variances for GLM and ANOVA models were confirmed using Proc Univariate (SAS v9.4) where a p ≤ 0.05 was considered significant. We found that Pekin ducks produce up to 16 different vocalizations. The treatments had a significant effect on the overall rate of calls given by the ducks (ANOVA: F6,31 = 8.55, p < 0.0001). Ducks produced the most calls by far when someone was sitting in the chamber with them (30.04 ± 4.45 calls/min). For call diversity, we found that there was a significant main effect of hen number (F218 = 12.21, p = 0.0004) but no main effect of drake number (F3,18 = 3.04, p = 0.0555). Cluster analyses indicated that certain types of calls were given under specific conditions. There were generally 6 major clusters of vocal repertoires (R-square = 0.899, Cubic Clustering Criterion = 9.30). Our results suggest that Pekin ducks are affected by the types of stimuli and social environment in how much they vocalize and in the properties of the calls they use. In addition, males and females differ somewhat in the repertoire of the calls they use, and in the spectral properties of their calls.

Preening cups in duck housing are associated with an increase in central dopamine activity that suggests a negative affective state.

Preening cups are a form of environmental enrichment that provides Pekin ducks a semi-open water source to express their natural behaviors. We recently observed that preening cups may increase feather pecking behaviors in ducks. Thus, we set out to determine if this form of enrichment can impact the affective state of Pekin ducks. To accomplish this goal, we evaluated the effect of preening cups on serotonin (5-HT) and dopamine (DA) turnover via mass spectrometry and their respective synthetic enzyme gene expression via qRT-PCR. Our study investigated the link between aggressive pecking with levels and activity of brain 5-HT and DA. Brain 5-HT and DA levels and activity have been established for decades to be associated with affective states. Grow-out Pekin ducks (n = 260) were housed at Purdue and raised per industry standards. On day 18, brains were collected from ducks in pens before preening cups were placed (PRE, n = 6) and, again on day 43, in pens with (PC, n = 6) and without (CON, n = 6) preening cups. Brains were dissected into right and left halves, then further microdissected into 4 brain areas: caudal mesencephalon (CM), rostral mesencephalon (RM), diencephalon (DI), and forebrain (FB). The right hemisphere was used for mass spectrometry to determine the neurotransmitter concentration (ng/mg of tissue) and those concentrations were applied to neurotransmitter turnover equations. There were no differences across treatments for 5-HT turnover in any brain area. There were differences in DA turnover across age (P = 0.0067) in the CM and across treatments (P = 0.003) in the RM. The left hemisphere of the brain was used to perform qRT-PCR on the genes of 5-HT and DA production enzymes. Within the CM, day 43 duck brains had increased (P = 0.022) tryptophan hydroxylase and tyrosine hydroxylase relative mRNA levels. All other brain areas showed no differences. Our data suggest that ducks housed with preening cups and that showed increased feather pecking are associated with increased brain DA activity. The increased DA in the brain may lead to a predisposition for increased aggression in the form of feather pecking.

Effects of different dietary vitamin D combinations during the grower phase and the feed restriction phase on growth performance and sternal morphology, mineralization, and related genes expression of bone metabolism in Pekin ducks.

Our study aimed to investigate the effects of different dietary vitamin D (VD) combinations during the grower (1-32 d of age) and feed restriction (33-52 d of age) phases on growth performance. We also evaluated sternal morphology, mineralization, and related genes expression of bone metabolism as well as absorption of calcium and phosphorous in duodenal mucosa and kidney in Pekin ducks. During the grower phase, we used 2 VD regimes (Group A: 3,160 IU/kg VD3; Group B: 400 IU/kg VD3 + 69 µg/kg 25-OH-D3). Each dietary treatment had 50 replicate pens of 10 ducks per pen. During the feed restriction phase, 30 replicate pens selected from Group A and Group B, repetitively, were redivided into 5 different dietary VD regimes to form a 2 × 5 experimental design. Each group consisted of 6 replicates, each with 10 ducks. During the feed restriction phase, we evaluated 5 different dietary VD combinations were as follows: T1: 2,000 IU/kg VD3 ; T2: 5,000 IU/kg VD3; T3: 3,620 IU/kg VD3 + 34.5 µg/kg 25-OH-D3; T4: 2,240 IU/kg VD3 + 69 µg/kg 25-OH-D3; T5: 1,800 IU/kg VD3 + 80 µg/kg 25-OH-D3). Results showed that Group B combinations with T5 had a better growth performance and breast meat deposition (P < 0.1). Regardless of 5 dietary VD regimes during the feed restriction phase, Group B significantly increased (P < 0.05) 52 d sternal depth and tended to increase (P < 0.1) 52 d sternal defatted weight, ash content, and phosphate (P) content of ducks. A significant interactive effect (P < 0.05) was observed on the mRNA abundance of DMP1 and Sost1 as well as RANKL/OPG in sternum and of VDR in duodenal mucosa of ducks at 52 d of age between dietary VD combinations during 2 phases. These results indicated that dietary VD regimes during the grower phase could affect the effectiveness of dietary VD regimes during the feed restriction phases; Dietary VD combinations of both phases could affect the genes expression of bone formation and the absorption as well as reabsorption of calcium and phosphorus in duodenum and kidney.

Heat stress and poultry production: a comprehensive review.

The impact of global warming on poultry production has gained significant attention over the years. However, our current knowledge and understanding of the mechanisms through which heat stress (HS) resulting from global warming affects the welfare, behavior, immune response, production performance, and even transgenerational effects in poultry are still incomplete. Further research is needed to delve deeper into these mechanisms to gain a comprehensive understanding. Numerous studies have investigated various biomarkers of stress in poultry, aiming to identify reliable markers that can accurately assess the physiological status and well-being of birds. However, there is a significant amount of variation and inconsistency in the results reported across different studies. This inconsistency highlights the need for more standardized methods and assays and a clearer understanding of the factors that influence these biomarkers in poultry. This review article specifically focuses on 3 main aspects: 1) the neuroendocrine and behavioral responses of poultry to HS, 2) the biomarkers of HS and 3) the impact of HS on poultry production that have been studied in poultry. By examining the neuroendocrine and behavioral changes exhibited by poultry under HS, we aim to gain insights into the physiological impact of elevated temperatures in poultry.

Preening cups increase apparent wet preening behaviors, but have no impact on other behaviors, body condition, growth, or body morphometrics of grow-out Pekin ducks.

Preening cups may be a form of open water that would allow ducks to express preening behaviors. We set out to test the hypothesis that preening cups would not have detrimental effects on ducks or their environment. Control pens (N = 6, 65 ducks/pen) had nipple lines while experimental pens (N = 6, 65 ducks/pen) had the same nipple line plus one preening cup (PC). Body weights of 30 ducks per pen, and body condition scores on 50 ducks per pen were recorded weekly. On d 18 and 43, 5 ducks per pen were euthanized and their spleens, Bursas, liver, and uropygial glands were weighed. Behavior data were collected using scan sampling with video being recorded for 72 continuous hours at 4 different ages: 25 d, 30 d, 36 d, and 40 d. Body morphometrics were analyzed by 2-way ANOVA with repeated measures. Body condition scoring was analyzed by Pearson's chi-square. The GLIMMIX procedure (SAS 9.4) was used for behavioral analyses to examine treatment differences in the proportion of ducks performing dry preening, wet preening, eating, drinking, standing, and laying down. Feather pecking, feather picking, preening conspecifics (also known as allopreening), dunking head, and drinking from preening cup were analyzed using PROC LOGISTIC with the Firth bias correction for quasi-complete separation and odds ratios were calculated. More PC ducks housed with PC performed wet preening compared to control ducks (25 d: F1,26 = 6.90, P = 0.0143; 30, 36, and 40 d; F1,78 = 24.53, P < 0.0001). Ducks in the PC group were also more likely to lay down compared to controls (25 d: F1,33 = 4.95, P = 0.0330). No differences were observed for any other behavior, body condition score, body weight or morphometrics at any age. Although ducks in the preening cup group showed an increase in wet preening, our data suggest that open water is not necessary to maintain feather condition or uropygial gland size.

Chronic treatment with glucocorticoids does not affect egg quality but increases cortisol deposition into egg albumen and elicits changes to the heterophil to lymphocyte ratio in a sex-dependent manner.

During chronic stress, there is an initial increase in glucocorticoid (GC) levels, but they then return to low, albeit not baseline, levels. Recent studies have renewed interest in cortisol in that it may also have important roles in the stress response. The purpose of our study was to test the hypothesis that chronic treatment with low levels of either corticosterone or cortisol would alter HLR and immune organ morphometrics. Further, we wanted to determine if chronic treatment with either GC would elicit an increase in cortisol levels in egg albumen. To test our hypotheses, we implanted silastic capsules that contained corticosterone, cortisol, or empty capsules as controls (N = 5/sex/treatment). Blood serum, smears, body weights, and egg quality data were collected. Ducks were then euthanized and body weight, weights of spleens, livers, and the number of active follicles were recorded. Albumen GC levels were assessed using mass spectrometry. Data were analyzed using a 2- or 3-way ANOVA as appropriate and post-hoc with Fishers PLSD. No treatment elicited differences in egg quality measures or body weight compared to controls. Corticosterone treatment did elicit an increase in serum corticosterone (p < 0.05), but not cortisol, levels compared to controls in both sexes. Both cortisol and corticosterone treatments increased (p < 0.05) serum levels of cortisol compared to controls. Relative spleen weights were higher (p < 0.05) in hens following corticosterone but not cortisol treatment. No other organs showed any differences among the treatment groups. Both GCs elicited an increase (p < 0.001) in HLR in hens at all time-points over the 2-week treatment period compared to controls. Cortisol, not corticosterone, only elicited an increase in HLR for drakes (p < 0.05) compared to controls but only at day 1 after implants. Chronic treatment with cortisol, but not corticosterone, elicited an increase (p < 0.01) in egg albumen cortisol levels compared to other groups. Corticosterone was not detected in any albumen samples. Our results suggest that glucocorticoids elicit differential effects and although corticosterone has been stated to be the predominant GC in avian species, cortisol may provide critical information to further understand bird welfare.

Research Note: Retinal cryptochrome gene expression is not altered by presence of light in incubators.

Cryptochromes are blue-, or ultraviolet-, light-absorbing proteins involved in the circadian clock, blue/ultraviolet light perception and potentially magnetoreception. At least 4 separate cryptochrome genes have been identified in avian species. The purpose of this study was to first determine if cryptochrome genes are expressed in the developing duck retina, and second to determine if the presence of lights in incubators affects the expression of cryptochrome genes. To accomplish these goals, duck eggs were placed in one of 2 commercial incubators (Buckeye, Single Stage Incubator, Model, SS-112) at Maple Leaf Farms, Inc., one with "poultry" LEDs obtained from a commercial source (Once Innovation, Agrishift) and the other in the absence of light (dark). Eggs in the incubators were placed on a reciprocating tray, tilting to 45° to simulate the rotation of eggs; thus all eggs spent 50% facing the light source and the other 50% of time facing 45° away from light source. Temperature gradients and humidity were maintained at industry standards. Retinal tissue samples from light and dark incubators were collected on days 3, 7, 11, 16, and 21 of incubation (extraction day, ED) known to be anatomical hallmarks of visual system development (n = 9-18 treatment group/ED timepoint). Samples were prepped and assayed for Cry2 and GAPDH gene transcription using qRT-PCR. Data were analyzed by using 2-ddCt method and a 2-way ANOVA was performed. No significant differences in Cry2 gene expression were observed between the lighted or dark incubator (P > 0.10). When combining light and dark treatment groups there is a significant 9 P < 0.05) increase in retinal Cry2 at ED 21, compared to ED 3 and 7. The presence of cryptochrome does not necessitate a migratory drive as evidenced by the fact that the Cry2 expression has been shown in non-migratory birds. However, since blue/ultraviolet wavelengths also activate the Cry2 photoreceptor, its presence could explain reports that suggest duck welfare can be improved if housed under lights that include ultraviolet wavelengths.

Chronic heat stress part 1: Decrease in egg quality, increase in cortisol levels in egg albumen, and reduction in fertility of breeder pekin ducks.

Global warming poses detrimental effects on poultry production leading to substantial economic losses. The goal of our experiment was to test the hypothesis that heat stress (HS) would alter welfare and egg quality (EQ) of breeder ducks. Furthermore, we wanted to test if HS would increase cortisol levels in egg albumen. Adult Pekin ducks were randomly assigned to two different rooms at 85% lay with 60 hens and 20 drakes per room. Baseline data including body weight, body condition scores (BCS), and egg production/quality were collected the week preceding heat treatment. Ducks were subjected to cyclic HS of 35°C for 10h/day and 29.5°C for the remaining 14h/day for 3 weeks while the control room was maintained at 22°C. Eggs were collected daily and analyzed weekly for quality assessment, and for albumen glucocorticoid (GCs) levels using mass spectrometry. One week before the exposure to HS, 10 hens and 5 drakes were euthanized and the same number again after 3 weeks and birds necropsied. Data analyses were done by 1- or 2-way ANOVA as appropriate with a Tukey-Kramer post hoc test. BCS were analyzed using a chi-squared test. A p ≤ 0.05 was considered significant. Circulating levels of corticosterone were significantly (p < 0.01) elevated at week 1 only in the HS hens. The circulating levels of cortisol increased significantly at week 1 and 2 (p < 0.05), and week 3 (p < 0.01) in the hens and at weeks 2 and 3 only (p < 0.05) in the drakes. Feather quality scores (p < 0.01), feather cleanliness scores (p < 0.001) and footpad quality scores (p < 0.05) increased significantly in the HS group. HS elicited a significant (p < 0.001) decrease in egg production at weeks 1 and 3. Hens in the HS group showed significantly decreased BW (p < 0.001) and number of follicles (p < 0.05). Shell weight decreased significantly at week 1 only (p < 0.05) compared to controls. Yolk weight decreased significantly at week 3 (p < 0.01) compared to controls. HS elicited a significant increase in albumen cortisol levels at week 1 (p < 0.05) and week 3 (p < 0.05). Thus, cortisol may provide critical information to further understand and to improve welfare.

Research Note: Sex difference in changes in heterophil to lymphocyte ratios in response to acute exposure of both corticosterone and cortisol in the Pekin duck.

Poultry scientists have utilized both direct and indirect measures of stress hormones for monitoring the state of avian welfare. For decades, it has been assumed that the mammalian and avian hypothalamic pituitary adrenal (HPA) function similarly to one another. However, there are considerable differences between the 2. Further, it has been assumed that the predominate glucocorticoid (GC) in birds was corticosterone, but recent studies have suggested that both corticosterone and cortisol are secreted. GC release is associated with an increase in blood heterophils due to increased migration from the lymph nodes and a decrease in lymphocytes due to marginalization. Both actions account for an increase in heterophil to lymphocyte ratios (HLR). The goal of this project was to determine the effect of each GC on HLR over time. To achieve this, we intramuscularly injected 2.0 mg/kg of corticosterone or cortisol, a lower dose cortisol treatment (0.5 mg/kg), or safflower oil as vehicle control. Blood was collected prior to intramuscular (IM) injections and blood collected 3 more times at every hour. Blood smears were also collected to assess HLR at the same four time points. HLR assays were completed by avian pathologists from an independent lab who were unaware of the treatments. Data were analyzed by 3-way repeated measures ANOVA with a P < 0.05 considered significant. We found significant sex (P < 0.001) x treatment (P < 0.001) x time (P < 0.001) effects with significant interactions (P = 0.0055). In hens, both GC resulted in significant increase in HLR at 1 h after injection compared to controls. In drakes, however, both GC showed a significant increase in HLR but not until 2 h after injection. The low dose cortisol had no significant effect on HLR in either sex. These data suggest that sex differences need to be considered when assessing duck welfare, and that cortisol may play a role in the HPA axis in ducks.

Sex differences in serum glucocorticoid levels and heterophil:lymphocyte ratios in adult pekin ducks (Anas platyrhynchos domesticus).

It is becoming more common for poultry scientists to utilize direct and indirect measures of stress hormones to monitor bird welfare. However, it has been clear that our understanding of the avian hypothalamic-pituitaryadrenal axis (HPA) is insufficient as evidenced by the many conflicting reports regarding stress responses, such as transportation stress, in poultry. It has long been assumed that the poultry HPA functions similarly to that of mammals, but now we know that there are considerable differences in the avian HPA compared to mammals. Synthesis and release of glucocorticoids (GC) are stimulated by adrenocorticotropin hormone (ACTH); GC are synthesized from a common pathway that begins with cholesterol and pregnenolone. The synthesis of one of the glucocorticoids does not depend upon the synthesis of the other. The purpose of our study was to test the hypothesis that ACTH will stimulate both corticosterone and cortisol release in ducks. To test this hypothesis, we injected artificial ACTH (cosyntropin; 0.0625 mg/kg, 0.031 mg/kg, or 0.016 mg/kg or saline as control) intramuscularly into adult drakes and hens (N = 10/sex/dose). Both glucocorticoids (GC) were assayed in serum using previously verified ELISAs. Blood smears were also assessed for heterophil to lymphocyte ratios (HLR). Data were analyzed by repeated measures 3-way ANOVA with Fishers PLSD as an ad hoc test. We observed that both GC were secreted in significantly (p = 0.0002) different patterns in a dose-dependent manner compared to controls, and that there was a significant (p = 0.0001) sex difference in both GC compared to saline controls. Further, we observed that all doses of ACTH elicited a significant (p = 0.004) sex difference in the HLR response compared to controls, but no dose-dependent effects were noted. Our data suggest that ducks, at least, may utilize more than just corticosterone to maintain physiological homeostasis in response to stress. Further, the time course of the stressor to release GC and subsequent HLR response may be dependent upon sex. More detailed analyses of the HPA are necessary in all avian species to better understand stress responses as we utilize biological bases for welfare assessments and stress responses.

Sex differences in glucocorticoid responses to shipping stress in Pekin ducks.

Some concerns have been raised recently about the assay of corticosterone vs. cortisol in poultry species. Thus, we tested the hypothesis that ducks secrete both glucocorticoids. First, we validated two commercially ELISA kits for the two glucocorticoids by first charcoal stripping duck serum in order to remove all steroid hormones. We ran serial dilutions of spiked, charcoal-stripped serum on kits of opposite glucocorticoid as well as a serial dilution using the respective ELISA buffer of the opposite assay kit. We found that the glucocorticoid standard curve in duck serum matched the respective curve in that kit's own buffer. However, when the opposite hormone was run in each kit in both duck serum or ELISA buffer, a near zero slope was obtained. Second, we further validated the presence of both glucocorticoids using mass spectrometry. Third, we tested the hypothesis that exogenous ACTH would stimulate the release of both corticosterone and cortisol. And, fourth, we tested the hypothesis that each glucocorticoid would have different serum levels in response to shipping stress. To test this hypothesis, we collected serum from 10 drakes and 10 hens from 2 flocks (N = 20 per time point per sex): 24 h prior to shipping, at shipping as ducks were walked off the truck, 24 h after shipping, and 1 wk after shipping. Data were analyzed by 2-way repeated measures ANOVA. Surprisingly, we also observed a sex difference in both glucocorticoid levels in that hens showed higher (P < 0.01) serum levels than did drakes at all-time points in response to either ACTH or transportation. Finally, no differences were observed in either glucocorticoid levels associated with shipping in either sex. The fact that both glucocorticoids are released in measurable amounts lends to the possibility that they may be differentially regulated, or at least there is a sex difference in the neural pathways associated with glucocorticoid release in ducks. Although corticosterone is the likely predominate glucocorticoid in ducks, serious attention should be given to the role of cortisol in poultry. Further consideration of sex, age, and timing of blood collection to stressor needs to be considered when assessing glucocorticoid levels in any avian species.

Centennial Review: History and husbandry recommendations for raising Pekin ducks in research or commercial production.

By some accounts, ducks were domesticated between 400 and 10,000 yr ago and have been a growing portion of the poultry industry for decades. Ducks specifically, and waterfowl in general, have unique health, housing, nutrition and welfare concerns compared to their galliform counterparts. Although there have been many research publications in regards to health, nutrition, behavior, and welfare of ducks there have been very few reviews to provide an overview of these numerous studies, and only one text has attempted to review all aspects of the duck industry, from breeders to meat ducks. This review covers incubation, hatching, housing, welfare, nutrition, and euthanasia and highlights the needs for additional research at all levels of duck production. The purpose of this review is to provide guidelines to raise and house ducks for research as specifically related to industry practices.

A feed restriction milieu for Pekin meat ducks that may improve gait characteristics but also affects gut leakiness.

In a previous study, we demonstrated that a 15% feed restriction (FR) during the first 2 wk after hatch could improve gait in Pekin meat ducks, but did result in reduced breast mass. We hypothesized that feed restriction after day 5 following muscle satellite cell development would allow the full growth of the breast meat. To accomplish this goal, 300 1-day-old ducklings (Maple Leaf Farms Inc.) were randomly allocated to 1 of the 3 groups (n = 4 pens, 25 ducks per pen): (1) Control group fed to ad libitum; (2) 85% daily feed intake from days 1 to 14 (FR 85% 1-14); 85% daily feed intake from days 5 to 14 (FR 85% 5-14). All ducks were vaccinated with inactivated Salmonella enteritidis on day 14 and boosted on day 26. The FR 85% 5-14 did show similar production standards to controls, and improved gait characteristics (P < 0.05). To determine if the partial feed restriction would have an impact on intestinal epithelial tight junction integrity, we treated ducks on days 7, 14, 21, 28, and 35 with 8.32 mg/kg FITC-d in water per os and blood samples were obtained via the tibial vein 1 h later. Serum samples were analyzed for presence and quantification of FITC-d. Feed restriction elicited a significant increase in FITC-d permeability at all points of evaluation. Anti-S. enteritidis specific IgY responses were assessed by ELISA from serum collected at 14 D, 28 D, and 35 D. Although all ducks showed an increase humoral immune response to the S. enteritidis, both feed restricted groups showed reduced IgY production compared to ad lib controls. Our data suggest that although the FR 5-14 feed restriction paradigm may reduce gait abnormalities without affecting production rates, some challenges exist due to increased gut leakiness or decreased acquired immune activity. Future studies will look at altering the feed restriction milieu to ameliorate these challenges.

Effect of dietary 25-hydroxycholecalciferol on the sternal mass of meat ducks under different vitamin regimens.

Genetic selection and intensive nutrition for increased growth rate in meat-type ducks has resulted in an imbalance between pectorales increment and sternal mass, which is detrimental to productivity and welfare. Reducing body weight and increasing sternal mass probably reverses these adverse effects. Therefore, 2 experiments (Expt.) were conducted to investigate the effects of 25-hydroxycholecalciferol (25-OH-D3), a vitamin D3 metabolites, on sternal mass. In Expt. 1, 512 1-day-old male ducks were randomly assigned to 4 low-nutrient density diets and received following treatments in a 2 × 2 factorial arrangement: (i) NRC or China Agricultural industry standards (NY/T) vitamin premixes and (ii) 0.069 mg/kg 25-HyD in feed or not. At 49 D of age, regardless of 25-OH-D3, NY/T vitamin regimen inhibited bone turnover and consequently increased sternal trabecular bone volume and mineral deposition compared with NRC vitamin premix. Supplementing 25-OH-D3 to NRC but not NY/T vitamin regimen significantly improved sternal microarchitecture and mineral content, which companied by decreased serum bone resorption markers concentration, as well as downregulation of the gene expressions of osteoclast differentiation and activity. In Expt. 2, 256 1-day-old male ducks were fed a standard nutrient density diet contained NRC vitamin premix with 0 or 0.069 mg/kg of 25-OH-D3. Results also showed that 25-OH-D3 treatment significantly improved sternal mineral accumulation and microarchitecture, along with decreasing osteoblast and osteoclast numbers in bone surface, declining serum bone turnover markers levels, and increasing serum Ca concentration. Collectively, these findings indicated that the dietary administration of 25-OH-D3 increased sternal mass in NRC vitamin diet by suppressing bone resorption in 49-day-old meat duck.

High vitamin levels ameliorate negative effect of rapeseed meal in meat ducks by improving antioxidant activity.

The purpose of this study was to test the hypothesis that a high level of a vitamin premix would prevent the deleterious effects of rapeseed meal (RSM) when added to the diet of Pekin meat ducks. A total of 674 fifteen-day-old ducks were randomly allocated to 6 treatments with 7 cages of 16 ducks each. Three diets were formulated that contained 5, 10, or 20% RSM to compensate for reducing levels of soybean meal. Each RSM level diet was then supplemented with either a low level (low) or a high level (high) of a vitamin premix providing a total of 6 experimental diets. Ducks were fed one of the 6 experimental diets (N = 7 pens per diet) from days 15 to 35 at which time they were euthanized. Ducks were analyzed for antioxidant activity, liver biochemistry, thyroid hormone levels, and liver and thyroid histopathology. Addition of the high vitamin premixes to the 5 or 10% RSM diets improved BW (P < 0.05), BW gain (BWG; P < 0.05), and feed to gain ratio (F/G; P < 0.05) compared to the low vitamin premix; however, neither vitamin premix level had effects on production variables of ducks fed the 20% RSM diet. The high vitamin premix level also improved antioxidant capacity as evidenced by increased (P < 0.05) serum and liver superoxide dismutase activities over that of the low vitamin premix diets. Furthermore, the high level of vitamin premix prevented liver and thyroid pathologies in diets that contain RSM compared to diets with the low vitamin premix. These results suggested that high vitamin premix could prevent the negative effects of a 5 or 10% RSM diet in ducks by improving antioxidative capacities and alleviating liver and thyroid damage.

Study on the morphology and mineralization of the tibia in meat ducks from 1 to 56 d.

The objective of this current study was to characterize the basic physiological data on morphological and mineralized properties of the tibia from 1 to 56 days, as well as the interrelationships of body weight (BW) and bone traits for meat ducks. A total of 176 1-d-old ducks was allocated into eight pens and fed with the same diet until the age of 56 d. Eight birds (1 ducks/pen) were randomly selected weekly for measuring BW and tibia characteristics. The BW of duck was increased with age throughout the whole study. Tibial length and width rapidly grew from 1 to 35 d, when it leveled off. The fat-free weight, ash, calcium (Ca) and phosphorus (P) content of tibia increased rapidly from 1 to 42 d and reached its plateau from 42 d onward. Tibial density and tibia-breaking strength increased in a regular manner with age until 42 d. Serum alkaline phosphatase activity was higher between 1 to 7 d in the duck. Regression and ontogenetic scaling analysis showed the age of maximal growth rate in tibia dimension and mineralization was earlier than that in BW; when compared with BW, tibial fat-free weight and tibia-breaking strength increased isometrically, tibia length displayed a negative allometry, and tibial width showed a positive allometric growth. Results indicate that the tibia displayed rapid bone growth (1 to 35 d) and mineralization (1 to 42 d), and alkaline phosphatase (ALP) plays a critical role in the early stage of tibia mineralization of meat duck. Intensive selection for growth rate in ducks has resulted in tibial morphology changes, which represents an interesting compromise of adaptation for increasing BW.

Effects of supplementation with different rapeseed oil sources and levels on production performance, egg quality, and serum parameters in laying hens.

This study was to determine the effects of rapeseed oil on production performance, egg quality, and serum parameters in laying hens. A total of 1,080 hens (33-wk-old) were randomly divided into a 1 plus 4 × 2 factorial design including four different rapeseed oil sources [high erucic acid of Mianyang city (MH); high erucic acid of Deyang city (DH); low erucic acid of Mianyang (ML); low erucic acid of Deyang (DL)] at two levels (2% and 4%) for 12 wk. The egg production and egg weight were decreased (P < 0.05) during 9 to 12 wk and 1 to 12 wk, while the average daily feed intake (ADFI) and feed conversion ratio were decreased (P < 0.01) in all phases compared to the control group. Adding ML as oil source had higher (P < 0.05) egg weight compared to DH in all periods in spite of levels. Meanwhile, layers fed 4% rapeseed oil decreased (P < 0.01) egg production compared with 2% in all phases except 1 to 4 wk. Regardless of rapeseed oil sources, hens fed 4% oil decreased (P < 0.05) egg weight in contrast to 2% during the whole experiment except 5 to 8 wk. The ADFI was lower (P < 0.01) in 4% oil inclusion groups compared with 2% during overall phase. Rapeseed oil decreased the yolk color (P < 0.01) and yolk ratio (P = 0.02) and increased (P < 0.01) the albumen height and Haugh unit at 12 wk. Dietary rapeseed oil supplementation resulted in a decreased total triglyceride (TG; P < 0.01) and increased high-density lipoprotein cholesterol (P = 0.02). Regardless of rapeseed oil levels, layers fed MH had higher TG (P < 0.01), TC (P < 0.05), low-density lipoprotein cholesterol (P < 0.05), alanine transaminase (P < 0.01) than those fed other sources. Taken together, the addition of rapeseed oil decreased laying performance, reduced TC and TG in the serum, and increased Haugh unit, with low erucic acid or 2% group showed more pronounced results among all treatments.

Low light intensity in Pekin duck breeder barns has a greater impact on the fertility of drakes than hens.

The Pekin duck is an excellent model for the study of seasonal reproduction. To more completely understand the lighting requirements for maximal fertility in duck breeder houses, we housed adult (45 week old) drakes and hens in the Hope College aviary as 5 drakes and 25 hens. Light conditions in each floor pen were normalized based upon quantal energy and divided into the following categories: (1) to simulate summer, 14.5 h 65 lux with 9.5 h 1 lux; (2) to simulate winter, 8 h 65 lux with 16 h 1 lux; (3) winter augmented, 8 h 65 lux with 16 h at 15 lux. The experiment was repeated with rotation of light treatments among 3 pens until a final N = 6 was obtained. Daily, total number of eggs laid was tallied, and a daily average of eggs laid was calculated throughout the study. Weekly, eggs were weighed and the perivitilline membrane was assayed for the number of sperm holes as an indirect measure of drake fertility. As expected, winter conditions caused a reduction in the percent of eggs laid and a reduction in the number of fertilized eggs compared to the summer light conditions. The augmented winter light conditions prevented the loss in the percent eggs laid and fertilized eggs. Surprisingly, even after 4 wk of the study, the winter conditions did not cause a complete loss of fertility in the Pekin ducks. At the end of the study, no differences in the relative expression in brain deep brain photoreceptors or gonadotropin inhibitory hormone mRNAs were observed among any light treatment. Although a minimum (1 lux) of light can support some fertility, our findings suggest commercial Pekin duck barns may benefit from increasing the augmented light to 15 lux to maintain optimal fertility during winter months. Furthermore, our data suggest that drakes may be more sensitive to environmental light conditions than hens.

Immunolesion of melanopsin neurons causes gonadal regression in Pekin drakes (Anas platyrhynchos domesticus).

Several light sensitive receptors have been described in the avian brain that are thought to regulate the reproductive axis independently from the eyes and pineal gland. Recently, our lab has described the presence of three of these photoneuroendocrine systems in the Pekin duck: opsin, opsin 5, & melanopsin. We set out to test the hypothesis that melanopsin receptive neurons are necessary to maintain seasonal reproductive status along with growth and development in the Pekin drake. To accomplish these goals we first investigated 50-week-old Pekin drakes that were housed in the aviary at Hope College under long day length (18h lights on) conditions in floor pens. To specifically lesion melanopsin-receptive neurons, 3µl of an anti-melanopsin-saporin conjugate (MSAP, 100ng/ul) was injected into the lateral ventricle (n=10). Control drakes were injected with 3µl of equimolar unconjugated anti-melanopsin and saporin (SAP, n=10). Reproductive behaviors were analyzed weekly in a test pen with adult hens and MSAP drakes showed a significant (p<0.01) reduction in reproductive behaviors after week 2. After 5weeks, drakes were euthanized and body weights were measured, and brains, pituitaries, and testes collected and stored for analyses. Mature MSAP-treated drakes had significantly (p<0.001) reduced relative teste weights compared to SAP controls. qRT-PCR analyses of hypothalamus showed a significant reduction (p<0.001) in GnRH and melanopsin mRNA levels, but not opsin 5, vertebrate ancient opsin, or opsin 2 (rhodopsin). Immunocytochemical analyses showed a significant reduction (p<0.01) in tyrosine hydroxylase-immunoreactivity in the PMM. These data suggest that although blue light alone is not able to maintain testicular function, the blue-light sensitive melanopsin activity is critical to maintain gonadal function.

Expression of deep brain photoreceptors in the Pekin drake: a possible role in the maintenance of testicular function.

Several putative deep brain photoreceptors (DBPs) have been identified, such as melanopsin, opsin 5, and vertebrate ancient opsin. The aim of this study was to elucidate the role of DBPs in gonadal regulation in the Pekin drake. As previously reported, we observed opsin-like immunoreactivity (-ir) in the lateral septum (LS), melanopsin-ir in the premammillary nucleus (PMM), and opsin 5-ir in the periventricular organ. To determine the sensitivity of the DBPs to specific wavelengths of light, drakes were given an acute exposure to red, blue, or white light. Blue light stimulated an increase (P < 0.01) in the immediate early gene fra-2-ir co-expression in melanopsin-ir neurons in the PMM, and red light increased (P < 0.05) fra-2-ir co-expression in opsin-ir neurons, suggesting these neurons are blue- and red-receptive, respectively. To further investigate this photoperiodic response, we exposed drakes to chronic red, long-day white, short-day white, or blue light. Blue light elicited gonadal regression, as testes weight (P < 0.001) and plasma luteinizing hormone (LH) levels (P < 0.001) were lower compared to drakes housed under long-day white light. Photo-regressed drakes experienced complete gonadal recrudescence when housed under long-day red and blue light. qRT-PCR analyses showed that gonadally regressed drakes showed reduced levels (P < 0.01) of gonadotropin releasing hormone (GnRH) mRNA but not photoreceptor or GnIH mRNAs compared to gonadally functional drakes. Our data suggest DBP in the LS may be rhodosin and multiple DBPs are required to fully maintain gonadal function in Pekin drakes.