RESUMEN
Scleral cartilaginous metaplasia was detected by routine histologic examination of globes from 5 Suffolk sheep from a scrapie pathogenesis study. The extent of the metaplasia varied among the sheep but was always posterior to the tapetal fundus. The matrix surrounding chondrocytes stained intensely with alcian blue and was immunopositive for type II collagen. Retrospective evaluation of additional eyes from Suffolk and Cheviot sheep used in various scrapie pathogenesis studies at the authors' facility revealed similar histologic changes in 40% and 12.7% of eyes examined, respectively. The clinical significance of this previously unreported finding is unknown.
Asunto(s)
Esclerótica/patología , Enfermedades de la Esclerótica/veterinaria , Enfermedades de las Ovejas/patología , Animales , Femenino , Metaplasia/patología , Metaplasia/veterinaria , Enfermedades de la Esclerótica/patología , OvinosAsunto(s)
Retina/fisiopatología , Scrapie/fisiopatología , Animales , Encéfalo/patología , Estudios de Casos y Controles , Modelos Animales de Enfermedad , Electrorretinografía/veterinaria , Eutanasia Animal , Proteína Ácida Fibrilar de la Glía/análisis , Inmunohistoquímica , Nervio Óptico/química , Proteínas PrPSc/análisis , Retina/química , Retina/inmunología , Scrapie/diagnóstico , OvinosRESUMEN
The testes and the spermatic cord of raccoons (Procyon lotor, kits to adult breeders; n = 48) were examined. Segmental arteritis confined to the extratesticular portions of the testicular artery was present in raccoons of all ages. The arterial changes were seen in laboratory-confined experimental and control animals as well as in wild-caught raccoons. The lesions consisted of proliferative endarteritis with presence of inflammatory cells within the intima, media, and the adventitial regions of most affected vessels. Some aspects of the proliferative arterial lesions were reminiscent of systemic necrotizing vasculitis (polyarteritis nodosa), an immunologically mediated condition of animals and humans. Etiologic agents were not identified at the affected sites. Arteritis was not attributed to the administration of infectious agents because it was present in raccoons of all age and origin. To our knowledge multifocal arteritis confined to the testicular artery has not previously been documented in raccoons.
Asunto(s)
Arteritis/veterinaria , Mapaches , Testículo/irrigación sanguínea , Animales , Arteritis/patología , MasculinoRESUMEN
Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. Susceptibility to the disease is partly dependent upon the genetic makeup of the host. In a previous study it was shown that sheep intracerebrally inoculated with US scrapie inoculum (No. 13-7) developed terminal disease within an average of 19 months. We have since produced an inoculum, No. x124 from pooled brains of US-origin sheep scrapie, that results in incubations nearly threefold shorter. The present study documents clinicopathologic findings and the distribution of abnormal prion proteins (PrP(Sc)) by immunohistochemical (IHC) and Western blot (WB) techniques, in tissues of sheep inoculated with No. x124. All inoculated sheep developed clinical disease and were euthanatized within an average of 7.7 months postinoculation (MPI). Sheep that had valine/valine or alamine/valine at codon 136 of prion protein (PRNP) gene developed the disease faster and were euthanatized at an average of 4.3 and 5.6 MPI, respectively. Also, the inoculum was able to induce disease in a short time (7 MPI) in a sheep that was relatively resistant (QR at codon 171) to scrapie. This indicates that inoculum No. x124 appears to induce scrapie in shorter time than inoculum No. 13-7, especially in sheep homozygous or heterozygous for valine at codon 136.
Asunto(s)
Priones/metabolismo , Scrapie/patología , Animales , Predisposición Genética a la Enfermedad , Hipopituitarismo , Masculino , Priones/genética , Scrapie/genética , Estados Unidos/epidemiologíaRESUMEN
Transmissible spongiform encephalopathies (TSEs) are a group of diseases that result in progressive and invariably fatal neurologic disease in both animals and humans. TSEs are characterized by the accumulation of an abnormal protease-resistant form of the prion protein in the central nervous system. Transmission of infectious TSEs is believed to occur via ingestion of prion protein-contaminated material. This material is also involved in the transmission of bovine spongiform encephalopathy ("mad cow disease") to humans, which resulted in the variant form of Creutzfeldt-Jakob disease. Abnormal prion protein has been reported in the retina of TSE-affected cattle, but despite these observations, the specific effect of abnormal prion protein on retinal morphology and function has not been assessed. The objective of this study was to identify and characterize potential functional and morphologic abnormalities in the retinas of cattle infected with a bovine-adapted isolate of transmissible mink encephalopathy. We used electroretinography and immunohistochemistry to examine retinas from 10 noninoculated and 5 transmissible mink encephalopathy-inoculated adult Holstein steers. Here we show altered retinal function, as evidenced by prolonged implicit time of the electroretinogram b-wave, in transmissible mink encephalopathy-infected cattle before the onset of clinical illness. We also demonstrate disruption of rod bipolar cell synaptic terminals, indicated by decreased immunoreactivity for the alpha isoform of protein kinase C and vesicular glutamate transporter 1, and activation of Müller glia, as evidenced by increased glial fibrillary acidic protein and glutamine synthetase expression, in the retinas of these cattle at the time of euthanasia due to clinical deterioration. This is the first study to identify both functional and morphologic alterations in the retinas of TSE-infected cattle. Our results support future efforts to focus on the retina for the development of new strategies for the diagnosis of TSEs.
Asunto(s)
Enfermedades de los Bovinos/virología , Oftalmopatías/veterinaria , Enfermedades por Prión/veterinaria , Priones/inmunología , Animales , Bovinos , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/patología , Electrorretinografía/veterinaria , Oftalmopatías/inmunología , Oftalmopatías/patología , Oftalmopatías/virología , Proteína Ácida Fibrilar de la Glía/inmunología , Transportador de Glucosa de Tipo 1/inmunología , Glutamato-Amoníaco Ligasa/inmunología , Inmunohistoquímica/veterinaria , Masculino , Enfermedades por Prión/inmunología , Enfermedades por Prión/patología , Enfermedades por Prión/virología , Proteína Quinasa C-alfa/inmunología , Células Fotorreceptoras Retinianas Bastones/inmunología , Células Fotorreceptoras Retinianas Bastones/patología , Células Fotorreceptoras Retinianas Bastones/virologíaRESUMEN
Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. Susceptibility to the disease is partly dependent upon the genetic makeup of the host. In a recent study, it was shown that sheep intracerebrally inoculated with a US scrapie agent (No. 13-7) developed scrapie and survived for an average of 19 months post inoculation. In the present study, when this scrapie inoculum was further passaged for 3 successive generations, the survival time was reduced by approximately 8 months in scrapie-susceptible (QQ on prion protein gene [PRNP] at codon 171) Suffolk sheep. It is concluded that inoculum No. 13-7 appears to have been stabilized in susceptible (171 QQ) Suffolk sheep and may be considered a specific isolate of sheep scrapie agent in the USA and therefore that it can be used to evaluate other isolates of sheep scrapie in this country.
Asunto(s)
Predisposición Genética a la Enfermedad/genética , Priones/genética , Scrapie/genética , Pase Seriado/veterinaria , Animales , Western Blotting/veterinaria , Inmunohistoquímica/veterinaria , Ovinos , Análisis de SupervivenciaRESUMEN
Neoplasms affecting the central and peripheral nervous systems of wild animals are extremely rare. Described are clinical signs and pathologic and immunohistochemical findings in an adult female raccoon (Procyon lotor) with an astrocytoma that involved medulla, cervical spinal cord, and roots of the cervical spinal nerves. Microscopically, the neoplastic cells revealed some pleomorphism but generally had fusiform morphology and showed moderate numbers of mitotic figures. Glial fibrillary acidic protein was demonstrated within the neoplastic cells by immunohistochemistry. This appears to be the only reported case of astrocytoma that involved multiple anatomic sites in the central nervous system of this raccoon.
Asunto(s)
Astrocitoma/veterinaria , Neoplasias del Sistema Nervioso Central/veterinaria , Bulbo Raquídeo/patología , Mapaches , Médula Espinal/patología , Nervios Espinales/patología , Animales , Astrocitoma/patología , Neoplasias del Sistema Nervioso Central/patologíaRESUMEN
To compare clinical and pathologic findings of chronic wasting disease (CWD) in a natural host, 3 groups (n = 5) of white-tailed deer (WTD) fawns were intracerebrally inoculated with a CWD prion of WTD, mule deer, or elk origin. Three other uninoculated fawns served as controls. Approximately 10 months postinoculation (MPI), 1 deer from each of the 3 inoculated groups was necropsied and their tissues were examined for lesions of spongiform encephalopathy (SE) and for the presence of abnormal prion protein (PrP(d)) by immunohistochemistry (IHC) and Western blot (WB). The remaining deer were allowed to live until they developed clinical signs of the disease which began approximately 18 MPI. By 26 MPI, all deer were euthanatized on humane grounds. Obvious differences in clinical signs or the incubation periods were not observed between the 3 groups of deer given CWD. In 1 of 3 nonclinical deer euthanatized at 10 MPI, minimal microscopic lesions of SE were seen in the central nervous system (CNS) tissues, and PrP(d) was observed by IHC in tissues of all 3 deer. In the clinical deer, CNS lesions of SE and PrP(d) accumulations were more severe and extensive. It is concluded that the 3 sources of CWD prion did not induce significant differences in time to clinical disease or qualitative differences in signs or lesions in WTD. However, this observation does not imply that these CWD agents would necessarily behave similarly in other recipient species.
Asunto(s)
Encéfalo/patología , Ciervos , Enfermedad Debilitante Crónica/epidemiología , Animales , Codón , ADN/genética , ADN/aislamiento & purificación , Amplificación de Genes , Genotipo , Reacción en Cadena de la Polimerasa , Enfermedades por Prión/mortalidad , Enfermedades por Prión/transmisión , Enfermedades por Prión/veterinaria , Priones/genética , Análisis de Supervivencia , Enfermedad Debilitante Crónica/mortalidadRESUMEN
During development and subsequent field evaluation of an oral vaccinia-rabies glycoprotein (V-RG) recombinant virus vaccine, 53 adult porcupines (Erethizon dorsatum; 38 females and 15 males) were examined. Microscopic examinations revealed the presence of giant epitheloid cells in various tissues (adrenal glands, spleen, liver, and lungs) of 4 (11%) female animals. These giant cells were approximately 20 times the size of the surrounding cells of the parenchyma. The cells were found singly and were not associated with any inflammatory cellular infiltrate and appeared to be located within vascular lumina. Morphologically these cells were typical of uterine epitheloid trophoblasts. This is the first record of the presence of trophoblast-like cells in nongenital tissues of porcupines.
Asunto(s)
Puercoespines , Trofoblastos/citología , Animales , Corteza Cerebral/citología , Femenino , Masculino , Bulbo Raquídeo/citología , PennsylvaniaRESUMEN
To determine the transmissibility of chronic wasting disease (CWD) to fallow deer (Dama dama) and to provide information about clinical course, lesions and suitability of currently used diagnostic procedures for detection of CWD in this species, 13 fawns were inoculated intracerebrally with CWD brain suspension from elk (n=6) or white-tailed deer (n=7). Three other fawns were kept as uninfected controls. Three CWD-inoculated deer were killed 7.6 months post-inoculation (mpi). None had abnormal prion protein (PrPd) in their tissues. One sick deer died at 24 mpi and one deer without clinical signs was killed at 26 mpi. Both animals had a small focal accumulation of PrPd in the midbrain. Between 29 and 37 mpi, three other deer became sick and were killed. All had shown gradual decrease in appetite and some loss of body weight. Microscopical lesions of spongiform encephalopathy were not observed, but PrPd was detected in tissues of the central nervous system (CNS) by immunohistochemistry, western blot and by two commercially available rapid diagnostic tests. This study demonstrates that intracerebrally inoculated fallow deer amplified CWD PrPd from white-tailed deer and elk in the absence of lesions of spongiform encephalopathy. Four years after CWD inoculation, the remaining five inoculated and two control deer are alive and apparently healthy.
Asunto(s)
Encéfalo/metabolismo , Ciervos , Médula Espinal/metabolismo , Enfermedad Debilitante Crónica/transmisión , Animales , Western Blotting , Encéfalo/patología , ADN Viral/análisis , Susceptibilidad a Enfermedades , Transmisión de Enfermedad Infecciosa , Femenino , Inmunohistoquímica , Masculino , Reacción en Cadena de la Polimerasa , Priones/genética , Priones/metabolismo , Priones/patogenicidad , Pase Seriado , Médula Espinal/patología , Enfermedad Debilitante Crónica/metabolismo , Enfermedad Debilitante Crónica/patologíaRESUMEN
Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. This study documents incubation periods, pathologic findings, and distribution of abnormal prion proteins (PrP(Sc)) by immunohistochemistry in tissues of genetically susceptible sheep inoculated with US sheep scrapie agent. Four-month-old Suffolk lambs (QQ at codon 171) were inoculated by 1 of 3 different routes (nasal, peritoneal, and conjunctival) with an inoculum (No. 13-7) consisting of a pool of scrapie-affected sheep brains. Except for 3 sheep, all inoculated animals were euthanized when advanced clinical signs of scrapie were observed between 19 and 46 months postinoculation (MPI). Spongiform lesions in the brains and labeling of PrP(Sc) in central nervous system and lymphoid tissues were present in these sheep. One intranasally inoculated sheep euthanized at 12 MPI had presence of PrP(Sc) that was confined to the pharyngeal tonsil. These results indicate that the upper respiratory tract, specifically the pharyngeal tonsil, may serve as a portal of entry for prion protein in scrapie-infected environments.
Asunto(s)
Conjuntiva , Predisposición Genética a la Enfermedad , Nariz , Peritoneo , Priones , Scrapie/genética , Scrapie/transmisión , Animales , Encéfalo , Femenino , Inyecciones , Masculino , Ovinos , Estados UnidosRESUMEN
Transmissible spongiform encephalopathies (TSEs) are a group of fatal neurodegenerative diseases characterized microscopically by spongiform lesions (vacuolation) in the neuropil, neuronal loss, and gliosis. Accumulation of the abnormal form of the prion protein (PrP(Sc)) has been demonstrated in the retina of natural and non-natural TSE-affected hosts, with or without evidence of microscopically detectable retinal pathology. This study was conducted to investigate the effect of PrP(Sc) accumulation on retinal neurons in a natural host lacking overt microscopical evidence of retinal degeneration by comparing the distribution of retinal cell type-specific markers in control and scrapie-affected sheep. In retinas with PrP(Sc)-immunoreactivity, there was disruption of the normal immunoreactivity patterns of the alpha isoform of protein kinase C (PKCalpha) and vesicular glutamate transporter 1 (VGLUT1), markers of retinal bipolar cells. Altered immunoreactivity was also observed for microtubule-associated protein 2 (MAP2), a marker of a subset of retinal ganglion cells, and glutamine synthetase (GS), a marker of Müller glia. These results demonstrate alterations of immunoreactivity patterns for proteins associated with specific cell types in retinas with PrP(Sc) accumulation, despite an absence of microscopical evidence of retinal degeneration.
Asunto(s)
Proteínas PrPSc/metabolismo , Células Bipolares de la Retina/metabolismo , Células Ganglionares de la Retina/metabolismo , Scrapie/fisiopatología , Animales , Glutamato-Amoníaco Ligasa/biosíntesis , Inmunohistoquímica , Proteínas Asociadas a Microtúbulos/biosíntesis , Proteínas PrPSc/análisis , Proteína Quinasa C-alfa/biosíntesis , Células Bipolares de la Retina/patología , Células Ganglionares de la Retina/patología , Scrapie/metabolismo , Ovinos , Proteína 1 de Transporte Vesicular de Glutamato/biosíntesisRESUMEN
Two regulatory region polymorphisms in the prion gene of cattle have been reported to have an association with resistance to classical bovine spongiform encephalopathy (BSE). However, it is not known if this association also applies to other transmissible spongiform encephalopathies (TSE) in cattle. In this report, we compare the relationship between these 2 polymorphisms and resistance in cattle affected with naturally occurring atypical BSE as well as in cattle experimentally inoculated with either scrapie, chronic wasting disease, or transmissible mink encephalopathy. Our analysis revealed no association between genotype and resistance to atypical BSE or experimentally inoculated TSE. This indicates the promoter polymorphism correlation is specific to classical BSE and that atypical BSE and experimentally inoculated TSE are bypassing the site of influence of the polymorphisms. This genetic discrepancy demonstrates that atypical BSE progresses differently in the host relative to classical BSE. These results are consistent with the notion that atypical BSE originates spontaneously in cattle.
Asunto(s)
Enfermedades de los Bovinos/genética , Encefalopatía Espongiforme Bovina/genética , Polimorfismo Genético , Enfermedades por Prión/genética , Priones/genética , Animales , Bovinos , Susceptibilidad a Enfermedades/veterinaria , Predisposición Genética a la Enfermedad , Genotipo , Regiones Promotoras Genéticas , Especificidad de la EspecieRESUMEN
Fourteen, 3-month-old calves were intracerebrally inoculated with the agent of chronic wasting disease (CWD) from white-tailed deer (CWDwtd) to compare the clinical signs and neuropathologic findings with those of certain other transmissible spongiform encephalopathies (TSE, prion diseases) that have been shown to be experimentally transmissible to cattle (sheep scrapie, CWD of mule deer [CWDmd], bovine spongiform encephalopathy [BSE], and transmissible mink encephalopathy). Two uninoculated calves served as controls. Within 26 months postinoculation (MPI), 12 inoculated calves had lost considerable weight and eventually became recumbent. Of the 12 inoculated calves, 11 (92%) developed clinical signs. Although spongiform encephalopathy (SE) was not observed, abnormal prion protein (PrPd) was detected by immunohistochemistry (IHC) and Western blot (WB) in central nervous system tissues. The absence of SE with presence of PrPd has also been observed when other TSE agents (scrapie and CWDmd) were similarly inoculated into cattle. The IHC and WB findings suggest that the diagnostic techniques currently used to confirm BSE would detect CWDwtd in cattle, should it occur naturally. Also, the absence of SE and a distinctive IHC pattern of CWDwtd and CWDmd in cattle suggests that it should be possible to distinguish these conditions from other TSEs that have been experimentally transmitted to cattle.
Asunto(s)
Encéfalo/metabolismo , Enfermedades de los Bovinos/transmisión , Ciervos/metabolismo , Enfermedad Debilitante Crónica/transmisión , Animales , Western Blotting , Encéfalo/patología , Bovinos , Enfermedades de los Bovinos/patología , Susceptibilidad a Enfermedades , Inmunohistoquímica , Masculino , Pase Seriado , Enfermedad Debilitante Crónica/patologíaRESUMEN
AIMS: Experiments were designed to evaluate the potential of rumen-simulating conditions to reduce PrP(Sc) levels. METHODS AND RESULTS: Scrapie-positive brain material was incubated under rumen-simulating conditions. Time points were taken over a 24-h period and PrP(Sc) levels were analysed by Western blot. No loss of PrP(Sc) was observed over a 24-h time period. CONCLUSIONS: Our results indicate that a fully developed rumen fermentation does not provide significant protection against prion infection via the oral route. Developmental changes including senescence of immune system function or other developmental changes in the gastrointestinal tract are potential mechanisms by which relative bovine spongiform encephalopathy (BSE) susceptibility might vary with age. SIGNIFICANCE AND IMPACT OF THE STUDY: Epidemiology of the BSE outbreak in the United Kingdom indicates that younger animals were at higher risk of infection. The rumen undergoes pronounced developmental changes early in life, coinciding with the introduction of fibre into the diet. The timeframe of highest risk of infection overlaps the time in life prior to full rumen development. This work indicates that a fully developed rumen does not provide significant protection against prion infection via the oral route of infection. This result implicates other developmental changes that are responsible for the age-dependent susceptibility of cattle to BSE.
Asunto(s)
Química Encefálica , Proteínas PrPSc/análisis , Rumen/química , Rumen/microbiología , Scrapie/inmunología , Animales , Western Blotting , Bovinos , Extractos Celulares/química , Proteínas PrPSc/metabolismo , Oveja DomésticaRESUMEN
The purpose of this study was to characterize the patterns of PrP(Sc) immunoreactivity in the retinae of scrapie-affected sheep and to determine the extent of retinal pathology as indicated by glial fibrillary acidic protein immunoreactivity (GFAP-IR) of Müller glia. Sections from the retina of 13 experimentally inoculated scrapie-affected and 2 negative control sheep were examined with immunohistochemical staining for PrP(Sc), GFAP, and PrP(Sc)/GFAP double staining. GFAP-IR of Müller glia is suggestive of retinal pathology in the absence of morphologic abnormality detected by light microscopy. Sheep with the least amount of PrP(Sc) in the retina have multifocal punctate aggregates of prion staining in the outer half of the inner plexiform layer and rarely in the outer plexiform layer. In these retinae, GFAP-IR is not localized with prion accumulation, but rather is present in moderate numbers of Müller glia throughout the sections of retina examined. The majority of sheep with retinal accumulation of PrP(Sc) have intense, diffuse PrP(Sc) staining in both plexiform layers, with immunoreactivity in the cytoplasm of multiple ganglion cells and lesser amounts in the optic fiber layer and between nuclei in nuclear layers. This intense PrP(Sc) immunoreactivity is associated with diffuse, intense GFAP-IR that extends from the inner limiting membrane to the outer limiting membrane. This is the first report of a prion disease in a natural host that describes the accumulation of PrP(Sc) in retina associated with retinal pathology in the absence of overt morphologic changes indicative of retinal degeneration.
Asunto(s)
Priones/metabolismo , Retina/metabolismo , Retina/patología , Enfermedades de la Retina/veterinaria , Scrapie/metabolismo , Scrapie/patología , Animales , Proteína Ácida Fibrilar de la Glía/metabolismo , Neuroglía/metabolismo , Enfermedades de la Retina/patología , Ovinos , Regulación hacia ArribaRESUMEN
Acute toxoplasmosis was diagnosed in 3 gray squirrels (Sciurus carolensis) from Louisiana and Pennsylvania. The predominant lesion was multifocal necrosis in several organs, especially of the lymph nodes. Numerous Toxoplasma gondii tachyzoites were seen in lesions, and the diagnosis was confirmed immunohistochemically by reaction with polyclonal T. gondii-specific antibodies. Tissue cysts were seen in several organs, including lung alveoli. The presence of tissue cysts in alveoli of pet squirrels maybe of public health concern if tissue cysts excreted in nasal secretions are swallowed by children.
Asunto(s)
Enfermedades de los Roedores/diagnóstico , Sciuridae/parasitología , Toxoplasmosis Animal/diagnóstico , Animales , Femenino , Inmunohistoquímica/veterinaria , Hígado/parasitología , Hígado/patología , Louisiana , Pulmón/parasitología , Pulmón/patología , Ganglios Linfáticos/parasitología , Ganglios Linfáticos/patología , Masculino , Necrosis/veterinaria , Philadelphia , Enfermedades de los Roedores/parasitología , Enfermedades de los Roedores/patología , Toxoplasmosis Animal/parasitología , Toxoplasmosis Animal/patologíaRESUMEN
Chronic wasting disease (CWD), a transmissible spongiform encephalopathy (TSE) of deer and elk, is one of a group of fatal, neurologic diseases that affect several mammalian species, including human beings. Infection by the causative agent induces accumulations of an abnormal form of prion protein (PrPres) in nervous and lymphoid tissues. This report documents the presence of PrPres within ectopic lymphoid follicles in a kidney of a white-tailed deer that had been experimentally inoculated by the intracerebral route with CWD 10 months previously. The deer was nonclinical, but spongiform lesions characteristic of TSE were detected in tissues of the central nervous system (CNS) and PrPres was seen in CNS and in lymphoid tissues by immunohistochemistry. The demonstration of PrPres in lymphoid tissue in the kidney of this deer corroborates a recently published finding of PrPres in lymphoid follicles of organs other than CNS and lymphoid tissues in laboratory animals with TSE (scrapie).
Asunto(s)
Coristoma/metabolismo , Ciervos/metabolismo , Riñón/patología , Tejido Linfoide/metabolismo , Priones/metabolismo , Enfermedad Debilitante Crónica/metabolismo , Enfermedad Debilitante Crónica/patología , Animales , Priones/aislamiento & purificaciónRESUMEN
To compare clinicopathologic findings of transmissible mink encephalopathy (TME) with other transmissible spongiform encephalopathies (TSE, prion diseases) that have been shown to be experimentally transmissible to cattle (sheep scrapie and chronic wasting disease [CWD]), two groups of calves (n = 4 each) were intracerebrally inoculated with TME agents from two different sources (mink with TME and a steer with TME). Two uninoculated calves served as controls. Within 15.3 months postinoculation, all animals from both inoculated groups developed clinical signs of central nervous system (CNS) abnormality; their CNS tissues had microscopic spongiform encephalopathy (SE); and abnormal prion protein (PrP(res)) as detected in their CNS tissues by immunohistochemistry (IHC) and Western blot (WB) techniques. These findings demonstrate that intracerebrally inoculated cattle not only amplify TME PrP(res) but also develop clinical CNS signs and extensive lesions of SE. The latter has not been shown with other TSE agents (scrapie and CWD) similarly inoculated into cattle. The findings also suggest that the diagnostic techniques currently used for confirmation of bovine spongiform encephalopathy (BSE) would detect TME in cattle should it occur naturally. However, it would be a diagnostic challenge to differentiate TME in cattle from BSE by clinical signs, neuropathology, or the presence of PrP(res) by IHC and WB.
Asunto(s)
Encéfalo/patología , Enfermedades de los Bovinos/transmisión , Enfermedades por Prión/veterinaria , Priones/metabolismo , Animales , Bovinos , Masculino , Enfermedades por Prión/transmisiónRESUMEN
To compare clinicopathological findings in first and second passage chronic wasting disease (CWD(mule deer)) in cattle, six calves were inoculated intracerebrally with brain tissue derived from a first-passage CWD-affected calf in an earlier experiment. Two uninoculated calves served as controls. The inoculated animals began to lose both appetite and weight 10-12 months later, and five subsequently developed clinical signs of central nervous system (CNS) abnormality. By 16.5 months, all cattle had been subjected to euthanasia because of poor prognosis. None of the animals showed microscopical lesions of spongiform encephalopathy (SE) but PrP(res) was detected in their CNS tissues by immunohistochemistry (IHC) and rapid Western blot (WB) techniques. Thus, intracerebrally inoculated cattle not only amplified CWD PrP(res) from mule deer but also developed clinical CNS signs in the absence of SE lesions. This situation has also been shown to occur in cattle inoculated with the scrapie agent. The study confirmed that the diagnostic techniques currently used for diagnosis of bovine spongiform encephalopathy (BSE) in the US would detect CWD in cattle, should it occur naturally. Furthermore, it raised the possibility of distinguishing CWD from BSE in cattle, due to the absence of neuropathological lesions and to a distinctive multifocal distribution of PrP(res), as demonstrated by IHC which, in this study, appeared to be more sensitive than the WB technique.