RESUMEN
Store-operated Ca(2+) entry (SOCE) over the plasma membrane is activated by depletion of intracellular Ca(2+) stores and has only recently been shown to play a role in CNS processes like synaptic plasticity. However, the direct effect of SOCE on the excitability of neuronal networks in vitro and in vivo has never been determined. We confirmed the presence of SOCE and the expression of the calcium sensors STIM1 and STIM2, which convey information about the calcium load of the stores to channel proteins at the plasma membrane, in neurons and astrocytes. Inhibition of SOCE by pharmacological agents 2-APB and ML-9 reduced the steady-state neuronal Ca(2+) concentration, reduced network activity, and increased synchrony of primary neuronal cultures grown on multi-electrode arrays, which prompted us to elucidate the relative expression of STIM proteins in conditions of pathologic excitability. Both proteins were increased in brains of chronic epileptic rodents and strongly expressed in hippocampal specimens from medial temporal lobe epilepsy patients. Pharmacologic inhibition of SOCE in chronic epileptic hippocampal slices suppressed interictal spikes and rhythmized epileptic burst activity. Our results indicate that SOCE modulates the activity of neuronal networks in vitro and in vivo and delineates SOCE as a potential drug target.
Asunto(s)
Proteínas de Unión al Calcio/metabolismo , Calcio/metabolismo , Epilepsia del Lóbulo Temporal/metabolismo , Glicoproteínas de Membrana/metabolismo , Proteínas de la Membrana/metabolismo , Neuronas/metabolismo , Animales , Astrocitos/citología , Astrocitos/metabolismo , Moléculas de Adhesión Celular/metabolismo , Membrana Celular/metabolismo , Enfermedad Crónica , Corteza Entorrinal/citología , Corteza Entorrinal/fisiopatología , Epilepsia del Lóbulo Temporal/fisiopatología , Hipocampo/citología , Hipocampo/fisiopatología , Humanos , Proteínas de Neoplasias/metabolismo , Red Nerviosa/metabolismo , Red Nerviosa/fisiopatología , Neuronas/citología , Técnicas de Cultivo de Órganos , Cultivo Primario de Células , Ratas , Molécula de Interacción Estromal 1 , Molécula de Interacción Estromal 2RESUMEN
Neurological diseases frequently induce pathological changes of cerebrospinal fluid (CSF) that might secondarily influence brain activity, as the CSF-brain barrier is partially permeable. However, functional effects of CSF on neuronal network activity have not been specified to date. Here, we report that CSF specimens from patients with reduced Glasgow Coma Scale values caused by severe traumatic brain injury suppress synchronous activity of in vitro-generated neuronal networks in comparison with controls. We present evidence that underlying mechanisms include increased N-methyl-D-aspartate receptor activity mediated by a CSF fraction containing elevated amino acid concentrations. These proof-of-principle data suggest that determining effects of CSF specimens on neuronal network activity might be of diagnostic value.
Asunto(s)
Lesiones Encefálicas/líquido cefalorraquídeo , Lesiones Encefálicas/fisiopatología , Líquido Cefalorraquídeo/fisiología , Red Nerviosa/fisiología , Receptores de N-Metil-D-Aspartato/fisiología , Adolescente , Adulto , Anciano , Células Cultivadas , Líquido Cefalorraquídeo/metabolismo , Células Madre Embrionarias/fisiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Red Nerviosa/metabolismo , Receptores de N-Metil-D-Aspartato/biosíntesisRESUMEN
Soluble amyloid beta(1-42) (A beta(1-42)) peptide has recently been assigned a key role in early Alzheimer's disease (AD) pathophysiology accounting for synaptic dysfunction before amyloid plaque formation and neurodegeneration can occur. Following sublethal A beta(1-42) administration, we observed an acute but transient reduction of the spike and burst rate of spontaneously active cortical networks cultured on microelectrode arrays. This simple experimental system appears suitable for future long-term pharmacological and genetic studies of A beta(1-42) signaling, thus providing a valuable new tool in AD research.
Asunto(s)
Potenciales de Acción/efectos de los fármacos , Péptidos beta-Amiloides/metabolismo , Corteza Cerebral/citología , Red Nerviosa/efectos de los fármacos , Neuronas/efectos de los fármacos , Fragmentos de Péptidos/metabolismo , Potenciales de Acción/fisiología , Péptidos beta-Amiloides/administración & dosificación , Péptidos beta-Amiloides/farmacología , Animales , Técnicas de Cultivo de Célula , Electrofisiología , Microelectrodos , Red Nerviosa/fisiología , Neuronas/fisiología , Fragmentos de Péptidos/administración & dosificación , Fragmentos de Péptidos/farmacología , RatasRESUMEN
Impaired axonal regeneration is a common observation after central nervous system (CNS) injury. The stromal cell-derived factor-1, SDF-1/CXCL12, has previously been shown to promote axonal growth in the presence of potent chemorepellent molecules known to be important in nervous system development. Here, we report that treatment with SDF-1alpha is sufficient to overcome neurite outgrowth inhibition mediated by CNS myelin towards cultured postnatal dorsal root ganglion neurons. While we found both cognate SDF-1 receptors, CXCR4 and CXCR7/RDC1, to be coexpressed on myelin-sensitive dorsal root ganglion neurons, the distinct expression pattern of CXCR4 on growth cones and branching points of neurites suggests a function of this receptor in chemokine-mediated growth promotion and/or arborization. These in vitro findings were further corroborated as local intrathecal infusion of SDF-1 into spinal cord injury following thoracic dorsal hemisection resulted in enhanced sprouting of corticospinal tract axons into white and grey matter. Our findings indicate that SDF-1 receptor activation might constitute a novel therapeutic approach to promote axonal growth in the injured CNS.