Screening and Risk Evaluation of Organic Contaminants in an Urban Wetland Fed with Wastewater Effluents.

Wastewater release in an urban wetland in Mexico City entails discharging complex mixtures of hundreds of chemical substances into the aquatic system. Therefore, in order to identify the most important contaminants, a GC-MS screening method coupled with deconvolution software was used. LC50 values from ECOSAR and Toxic Units were obtained to identify the organic pollutants-of-concern and to pinpoint the most ecotoxic sites. The results showed that, even though the area has intensive farming, agrochemicals are not of concern. Industrial and domestic groups make up from about 30%-66% of the pollutants for all sites. Of the 189 identified substances, 17 were detected 44% of the time and the Toxic Unit showed that terbutol, coprostanol, and hexadecane were capable of generating considerable adverse effects on the aquatic ecosystem. Total Toxic Units for each site exhibited a decreasing trend as they distanced from the urban and agricultural area.

Cellular, physiological and molecular approaches to investigate the antifungal and anti-aflatoxigenic effects of thyme essential oil on Aspergillus flavus.

Several approaches, including the detection of apoptotic-like cell death, aflatoxin B1 (AFB1) production and gene expression analysis, were carried out to provide insights into the antifungal and anti-aflatoxigenic effects of thyme essential oil (EO) on Aspergillus flavus. At 0.5 µL mL-1, thyme EO completely inhibited A. flavus growth. Furthermore, this antifungal activity triggered significant apoptosis, via nuclear condensation (87.5% of nuclei analyzed) and plasma membrane damage (in 100% of treated hyphae). Further analysis of AFB1 production and gene expression related to secondary metabolism (laeA) and the mechanism of virulence (lipA and meT) of A. flavus in the presence of thyme EO indicated important physiological changes related to its anti-aflatoxigenic property. These results highlight the potent antifungal abilities of thyme EO in controlling A. flavus and AFB1 production, especially the abilities that operate by exerting changes at the molecular level and inducing significant apoptotic-like cell death.

Presence of unreported carcinogens, Aflatoxins and their hydroxylated metabolites, in industrialized Oaxaca cheese from Mexico City.

Aflatoxins (AFs) are toxic secondary metabolites of the fungi Aspergillus flavus, A. parasiticus and A. nomius. The fungi produce these AFs in cereals, oilseeds and spices. AFs have damaging effects on all organisms, including humans, and their symptoms can be classified as acute (vomiting, hemorrhage and death) or chronic (immunodepression, Reye syndrome, Kwashiorkor, teratogenesis, hepatitis, cirrhosis, and various cancers). Basic AFs (AFB1, AFB2, AFG1, and AFG2) are metabolized in the liver or by microbes that produce hydroxylated metabolites (AFM1, AFM2, and AFP1) and aflatoxicol (AFL), soluble in water and easy to dispose. Thus, AFs can be excreted in fluids, such as milk. AFs are not destroyed in the process of making cheese. The purpose of this study was to identify and quantify the AFs present in 30 samples of industrialized Oaxaca-type cheese sold in Mexico City. The average concentrations of AFs detected in the 30 samples of industrialized cheese were as follows: AFB1 (0.1 µg kg-1) in 20% (6/30); a trace amount of AFB2 (0.01 < LOD) in only 3% (1/30); AFG1 (0.14 µg kg-1) in 10% (3/30); AFG2 (0.6 µg kg-1) in 30% (9/30); AFM1 (1.7 µg kg-1) in 57% (17/30); AFP1 (0.03% µg kg-1) in 3% (1/30); and AFL (13.1 µg kg-1) in 97% (29/30). AFB1 and AFL were the most abundant aflatoxins in Oaxaca-type cheese. However, eight aflatoxins were present, contributing an average of 15.7 µg kg-1 AFs distributed among the 30 samples. The risk assessment analysis showed that there was no substantial risk for cancer due to AFs in industrialized Oaxaca cheese from Mexico City.

Longitudinal study of microbial diversity and seasonality in the Mexico City metropolitan area water supply system.

In the Mexico City metropolitan area (MCMA), 70% of the water for 18 million inhabitants is derived from the Basin of Mexico regional aquifer. To provide an overview of the quality of the groundwater, a longitudinal study was conducted, in which 30 sites were randomly selected from 1,575 registered extraction wells. Samples were taken before and after chlorine disinfection during both the rainy and dry seasons (2000-2001). Microbiological parameters (total coliforms, fecal coliforms, streptococci, and Vibrio spp.), the presence of Helicobacter pylori, and physicochemical parameters, including the amount of trihalomethanes (THMs), were determined. Although microorganisms and inorganic and organic compounds were evident, they did not exceed current permissible limits. Chlorine levels were low, and the bacterial counts were not affected by chlorine disinfection. Eighty-four bacterial species from nine genera normally associated with fecal contamination were identified in water samples. H. pylori was detected in at least 10% of the studied samples. About 40% of the samples surpassed the THM concentration allowed by Mexican and U.S. regulations, with levels of chloroform being high. The quality of the water distributed to the MCMA varied between the rainy and dry seasons, with higher levels of pH, nitrates, chloroform, bromodichloromethane, total organic carbon, and fecal streptococci during the dry season. This study showed that the groundwater distribution system is susceptible to contamination and that there is a need for a strict, year-round disinfection strategy to ensure adequate drinking-water quality. This situation in one of the world's megacities may reflect what is happening in large urban centers in developing countries which rely on a groundwater supply.

Optimization of carbohydrate silylation for gas chromatography.

We developed and optimized a new carbohydrate mono- and disaccharides silylation reaction, replacing pyridine and requiring lower reaction temperature and less time. Our method consists of three basic steps. The first one is oxime formation, the second one silylate derivative and the last one gas chromatography separation and quantification with an internal standard. We evaluated several solvents, including acetonitrile, hydroxylamine and aniline. We found aniline to be the best reaction media for oxime formation with hydroxylamine hydrochloride. Among silylation agents we found N,O-bis(trimethyl)trifluoroacetamide (BSTFA) was the most efficient. Together these reagents favored both a short analysis time and fewer by-products. We evaluated the method with model solutions containing: arabinose and co-eluting xylose, fructose, glucose, sucrose and salicin (internal standard) and found it suitable for processed food analysis.