Combining near-infrared hyperspectral imaging with elemental and isotopic analysis to discriminate farm-raised pacific white shrimp from high-salinity and low-salinity environments.

White shrimp (Litopenaeus vannamei) raised in low-salinity farm are considered inferior to those in seawater. In order to develop a rapid discrimination method for the food industry, we investigated the potential of using near-infrared hyperspectral imaging to discriminate shrimp muscle samples from freshwater and seawater farms. We constructed 3 different discrimination models with 4 optimal wavelength selection methods and compared the performance of each model. The results showed that sequential forward selection combined with partial least squares discriminant analysis (SFS-PLS-DA) generated the best discrimination performance with an overall accuracy of 99.2%. The elemental and isotopic analysis indicated a high correlation between 918 and 925 nm region (which was selected by SFS) and 13C concentration. This agrees with the fact that there is more 13C in shrimp of salty water compared to those of freshwater. The results demonstrated (hyperspectral imaging) HSI is promising to discriminate L. vannamei raised in fresh and seawater environments.

Using hyperspectral analysis as a potential high throughput phenotyping tool in GWAS for protein content of rice quality.

BACKGROUND: The advances of hyperspectral technology provide a new analytic means to decrease the gap of phenomics and genomics caused by the fast development of plant genomics with the next generation sequencing technology. Through hyperspectral technology, it is possible to phenotype the biochemical attributes of rice seeds and use the data for GWAS. RESULTS: The results of correlation analysis indicated that Normalized Difference Spectral Index (NDSI) had high correlation with protein content (PC) with RNDSI 2 = 0.68. Based on GWAS analysis using all the traits, NDSI was able to identify the same SNP loci as rice protein content that was measured by traditional methods. In total, hyperspectral trait NDSI identified all the 43 genes that were identified by biochemical trait PC. NDSI identified 1 extra SNP marker on chromosome 1, which annotated extra 22 genes that were not identified by PC. Kegg annotation results showed that traits NDSI annotated 3 pathways that are exactly the same as PC. The cysteine and methionine metabolic pathway identified by both NDSI and PC was reported important for biosynthesis and metabolism of some of amino acids/protein in rice seeds. CONCLUSION: This study combined hyperspectral technology and GWAS analysis to dissect PC of rice seeds, which was high throughput and proven to be able to apply to GWAS as a new phenotyping tool. It provided a new means to phenotype one of the important biochemical traits for the determination of rice quality that could be used for genetic studies.