Age-related features of Cytoflavin effectiveness during experimental myocardial ischemia.

Administration of Cytoflavin to young rats with experimental myocardial ischemia was followed by activation of mitochondrial enzymes and significant accumulation of ATP in the myocardium and blood plasma, but did not modulate the stability of cardiomyocyte membranes and signs of hypoxia. Cytoflavin increased the severity of metabolic disorders and depleted the energy reserves of cells in old rats with myocardial ischemia.

[Efficiency of mildronate in rats of different age with experimental-induced myocardial ischemia].

Under experimental myocardial ischemia in rats of 10 months treatment with mildronate resulted in essential changes in metabolism of cardiomyocites. This includes stimulation of aerobic and anaerobic ways of power supply of heart cells: activation of glycolysis, oxidative phosphorylation and oxidative pyruvate decarboxylation with restoration of adenosine triphosphate pool to intact rats level in myocardium, serum and erythrocytes with signs of stabilization of cardiomyocytes membranes and essential decrease of tissue hypoxia. Introduction of mildronate to old rats (24 months) with an experimental myocardium ischemia was accompanied by lesser expressed changes of metabolism: activation of glycolysis and oxidative pyruvate decarboxylation without stimulation of Crebs' cycle enzymes. This became sufficient for restoration of adenosine triphosphate pool in myocardium without change of its quantity in serum and erythrocytes with signs of stabilization of cardiomyocytes membranes and moderate reduction of tissue hypoxia degree.

[Efficiency of trimetazidine treatment of experimental ischemic heart disease in age aspect].

Introduction of trimetazidine to 10-month-aged rats with experimental ischemic heart disease leads to an increase in carbohydrate utilization with energy purposes in myocardium, which is manifested by increasing activity of glycolysis enzymes with decreasing lactate level in myocardium, increasing activity of pyruvate dehydrogenase and citrate synthase in mitochondrial cardiomyocytes, and increasing ATP content in myocardium. This is accompanied by signs of stabilization of cardiomyocyte membranes and reduction in the degree of tissue hypoxia. The efficiency of trimetazidine decreases with increasing age: in 24-month-aged rats, the direction of changes is retained, but they are less pronounced.

[Erythrone system in aged patients with chronic cardiac insufficiency].

Basic mechanisms underlying the development of chronic cardiac insufficiency (CCI) in aged subjects were investigated in a randomized study of 30 patients with I-IV functional class CCI and preserved left ventricular systolic function. CCI affected the left side of the heart in 23 patients due to concurrent hypertensive disease and the right side in 7 patients with concomitant chronic broncho-pulmonary pathology. Mean age of the patients was 65 years. Control group comprised 8 practically healthy middle-age subjects. Erythrone system condition was evaluated from erythrocyte morphological characteristics and chemical composition studied by scanning electron microscopy on a FEI quanta 2003D apparatus in the Nanotechnologies and Nanomaterials Centre, Belgorod State University. Pathological changes in the erythrone system of CCI patients included changes of erythrocyte shape and chemical composition especially well apparent in cases with chronic pulmonary heart. As CCI progressed, erythrocytes in the form of bi-convex disks turned to spheres with the papillose surface. The sphere to disk ratio in the compensation, sub- and decompensation stages was 1:7, 1:4, and 9:1 respectively compared with 1:60 in controls. Spherocytes contained less nitrogen than normal diskocytes. Protein degradation was more apparent in aged CCI patients. Their erythrocytes contained 4.5-5, 2, 3 and 4 times more calcium, magnesium, aluminium, and silicon respectively than the cells of healthy subjects. It suggests development of calcium paradox, abnormal membrane permeability and gradual cell death (hemolysis). Anemia in aged CCI patients appears be of haemolytic nature. It occurred in 6.7-11.5% of the cases of left ventricular CCI and in 33.3% of the aged patients with chronic pulmonary heart, the lethality rate being 0.93 and 28.6% respectively. It is concluded that changes in the erythrone system in the form of erythrocyte spherulation, accumulation of calcium and other chemical elements suggest a poor prognosis of CCI in elderly patients.

[The prognosis for the occurrence of inflammatory genital diseases in girls and young women]. / Prohnozuvannia vynyknennia zapal'nykh zakhvoriuvan' statevykh orhaniv u divchat ta iunykh zhinok.

With the purpose of predicting probability of origination of inflammatory diseases of the genital organs among young female patients and their diagnosis during the early stages of development thereof, an algorithm has been worked out together with a mathematical model of probability of origination of genitalia inflammatory diseases at young age, based on prevailing social and economic and medical and biological risk factors. Correspondence between the secured indices across all samples among girls came up to 95.9%, that for the young women group was 99.3%. High significance and relative simplicity of the above algorithm for prediction of risk of development of inflammatory diseases of genitalia in girls and young women suggest to us that it can surely come into widespread use.

[Analysis of the reasons for insufficient efficacy of treating inflammatory diseases of the urogenital system in women]. / Analiz prichin nedostatochnoi éffektivnosti lecheniia vospalitel'nykhzabolevanii urogenital'noi sistemy u zhenshchin.

Concentration of tetracycline, doxicyclin and tarivide was studied in blood serum, urine and immediately in the impaired focus, in uterine tube tissue to elucidate the factors responsible for low therapeutic efficiency of drugs involved in treatment of inflammatory diseases of women urogenital system. Content of tetracycline, detected in the patients blood and urine, varied within the limits 0.02-0.12 microgram/ml and 0.003-0.136 microgram/ml, respectively, depending on the period of administration of therapeutic doses of the antibiotic. At the same time, in uterine tube tissue concentration of tetracycline was only slightly increased and constituted 0.014 microgram/ml which was distinctly lower than the minimal inhibitory content for the antibiotic. Similar results were obtained in studies of tarivide concentrations. Both morphofunctional impairments of uterine tubes and deficiency of antibiotics in the impaired focus were responsible for inadequate treatment of women with infertility of inflammatory genesis.