ABSTRACT
River buffalo (Bubalus bubalis, 2n = 50, BBU) is a species of economic relevance in a number of countries. This species shows a very peculiar biology and a great capacity for environmental adaptation. There has been an increasing economic interest as well as a growing demand for a more detailed knowledge of molecular features in this species. From this perspective we report a genomic, transcriptional and cytogenetic analysis of 5 master genes involved in skeletal muscle development. Of these 5 genes, MYOD1, MYF5, MYF6 and MYOG belong to the basic helix-loop helix protein family while MSTN belongs to the TNF-B protein family. In mammals, these genes are involved in the early stages of skeletal muscle differentiation, development and regeneration. These pivotal biological functions are finely regulated in a tissue- and temporal-specific manner. We used a comparative genomic approach to obtain the buffalo specific sequences of MYOD1 and MYF6. The nucleotide sequence similarity and the protein domain conservation of the newly obtained sequences are analysed with respect to bovine and other mammalian species showing sequence similarity. The presence of a polymorphism in MYOD1 coding sequence is described and its possible effect discussed. Using a quantitative PCR approach, we compared the level of the 5 transcripts in adult and fetal muscle. These genes were physically localised on river buffalo R-banded chromosomes by FISH using bovine genomic BAC-clones. Here, we present a genomic and cytogenetic analysis which could offer a background to better characterise the buffalo genes involved in muscle function and which may be responsible for buffalo-specific meat features.
Subject(s)
Buffaloes/genetics , Chromosome Mapping , Muscle, Skeletal/physiology , Acclimatization , Animals , Buffaloes/physiology , Cattle , Cell Differentiation , Cloning, Molecular , Computational Biology , DNA/genetics , DNA Primers , Environment , Genotype , In Situ Hybridization, Fluorescence , Muscle, Skeletal/cytology , MyoD Protein/genetics , Myogenic Regulatory Factors/genetics , Myostatin/genetics , Polymorphism, Genetic , Species SpecificityABSTRACT
Epigenetic regulation is involved in the maintenance of long-term silencing phenomena, such as X-inactivation and genomic imprinting in mammals. Gene repression is mediated by several mechanisms, such as histone modifications, DNA methylation, and recruitment of Polycomb proteins. To understand the mechanistic relationships between these mechanisms for stable gene silencing, we analyzed the mechanisms of X- and Y-inactivation of the PAR2 gene SYBL1, previously showed to be regulated by concerted epigenetic mechanisms. Maintenance of stable repression occurs via the recruitment of both MBDPs and PRC2 complexes to SYBL1 promoter. Their binding is equally sensitive to defective DNA methylation seen in cells derived from ICF syndrome patients. Multiple occupancy is a feature shared within long-term repressed genes, such as the X-inactivated PGK1 and the imprinted IGF2. MBD2, MBD3, and MeCP2 occupy SYBL1 promoter simultaneously, as revealed by sequential ChIP. We did not find this co-occurring binding when looked for members of PRC2 complex together with any of the methyl-binding proteins. Furthermore, in co-transfection assays, MECP2 can silence methylated SYBL1 promoter, whereas the mutated protein fails. However, RNA interference of endogenous MECP2 does not induce the expression of the inactive SYBL1 alleles, suggesting that its silencing activity can be replaced by the other methyl-binding proteins. Our data suggest that maintenance of long-term silencing involves multiple layers of epigenetic control functionally redundant. PRC2 and MBD proteins could collaborate to different phases of this process, the former possibly recruiting DNMTs to the silenced promoters, the latter dictating the lock of the transcription.
Subject(s)
DNA-Binding Proteins/metabolism , Gene Silencing/physiology , Methyl-CpG-Binding Protein 2/metabolism , Repressor Proteins/metabolism , Transcription Factors/metabolism , Cell Line , DNA Methylation , Enhancer of Zeste Homolog 2 Protein , Female , Gene Expression Regulation/physiology , Humans , Insulin-Like Growth Factor II , Polycomb Repressive Complex 2 , Polycomb-Group Proteins , Promoter Regions, Genetic/physiology , Protein Binding , Proteins/genetics , Proteins/metabolism , R-SNARE Proteins/genetics , R-SNARE Proteins/metabolism , RNA Interference/physiology , RNA, Small Interfering/physiologyABSTRACT
Maintenance of X-inactivation is achieved through a combination of different repressive mechanisms, thus perpetuating the silencing message through many cell generations. The second human X-Y pseudoautosomal region 2 (PAR2) is a useful model to explore the features and internal relationships of the epigenetic circuits involved in this phenomenon. Recently, we demonstrated that DNA methylation plays an essential role for the maintenance of X- and Y-inactivation of the PAR2 gene SYBL1; here we report that the silencing of the second repressed PAR2 gene, SPRY3, appears to be independent of DNA methylation. In contrast to SYBL1, the inactive X and Y alleles of SPRY3 are not reactivated in cells treated with a DNA methylation inhibitor and in cells from ICF (immunodeficiency, centromeric instability, facial anomalies) syndrome patients, which have mutations in the DNA methyltransferase gene DNMT3B. SPRY3 X- and Y-inactivation is associated with a differential enrichment of repressive histone modifications and the recruitment of Polycomb 2 group proteins compared to the active X allele. Another major factor in SPRY3 repression is late replication; the inactive X and Y alleles of SPRY3 have delayed replication relative to the active X allele, even in ICF syndrome cells where the closely linked SYBL1 gene is reactivated and advanced in replication. The relatively stable maintenance of SPRY3 silencing compared with SYBL1 suggests that genes without CpG islands may be less prone to reactivation than previously thought and that genes with CpG islands require promoter methylation as an additional layer of repression.
Subject(s)
Chromosomes, Human, X/metabolism , Chromosomes, Human, Y/metabolism , DNA Methylation , Epigenesis, Genetic , Proteins/metabolism , Alleles , Cell Line, Transformed , Chromosomes, Human, X/genetics , Chromosomes, Human, Y/genetics , DNA Replication , Female , Fibroblasts/metabolism , Gene Expression Regulation , Histones/metabolism , Humans , Intracellular Signaling Peptides and Proteins , Male , Models, Genetic , Proteins/geneticsABSTRACT
Reverse-transcriptase polymerase chain reaction (RT-PCR) with multiple markers has been demonstrated to be highly sensitive in detecting metastatic cells in peripheral blood of malignant melanoma (MM) patients, and the circulating MM cells to be significantly correlated with disease stages. We further evaluated the presence of specific PCR-positive mRNA markers in peripheral blood as well as in regional nodes as an expression of tumor progression. Peripheral blood samples from 317 MM patients with either localized (n = 219) or metastatic (n = 98) disease were processed to obtain total cellular RNA. RT-PCR was performed using tyrosinase (TYR), p97, and MelanA/MART1 as mRNA markers. PCR products were analyzed by gel electrophoresis and Southern blot hybridization. In addition, paraffin-embedded samples of histologically proven tumor-negative lymph nodes from the subset of patients with localized disease were analyzed by RT-PCR, using radiolabeled primers for TYR and MelanA/MART1. The presence of mRNA markers was significantly correlated with tumor burden with a good correlation between risk of recurrence (evaluated in stage I-III patients) and increasing number of PCR-positive markers (p = 0.0002). Currently, for each patient, PCR results obtained at different times during follow-up are being analyzed, and any variation in the number of PCR-positive markers is being correlated to the clinical status. Molecular screening of histologically negative nodes for the presence of metastatic MM cells is also under evaluation. Preliminary assessment of a subset of MM patients with higher risk of recurrence will require longer follow-up in order to define the role of RT-PCR in monitoring these patients.
Subject(s)
Biomarkers, Tumor/blood , Lymph Nodes/pathology , Melanoma/blood , Neoplasm Proteins/blood , Neoplastic Cells, Circulating , RNA, Messenger/analysis , Skin Neoplasms/blood , Biomarkers, Tumor/genetics , Blotting, Southern , Disease Progression , Follow-Up Studies , Humans , Neoplasm Proteins/genetics , Neoplasm Staging , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Malignant melanoma (MM) is thought to arise by sequential accumulation of genetic alterations in normal melanocytes. Previous cytogenetic and molecular studies indicated the 9p21 as the chromosomal region involved in MM pathogenesis. In addition to the CDKN genes (p16/CDKN2A, p15/CDKN2B and p19(ARF), frequently inactivated in familial MM), widely reported data suggested the presence within this region of other melanoma susceptibility gene(s). To clearly assess the role of the 9p21 region in sporadic melanoma, we evaluated the presence of microsatellite instability (MSI) and loss of heterozygosity (LOH) in primary tumours as well as in synchronous or asynchronous metastases obtained from the same MM patients, using 9 polymorphic markers from a 17-cM region at 9p21. LOH and MSI were found in 27 (41%) and 11 (17%), respectively, out of 66 primary tumours analysed. In corresponding 58 metastases, MSI was found at higher rate (22; 38%), whereas a quite identical pattern of allelic deletions with 27 (47%) LOH+ cases were observed. Although the CDKN locus was mostly affected by LOH, an additional region of common allelic deletion corresponding to marker D9S171 was also identified. No significant statistical correlation between any 9p21 genetic alteration (LOH, MSI or both) and clinicopathological parameters was observed.
Subject(s)
Chromosomes, Human, Pair 9/genetics , Melanoma/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Analysis of Variance , DNA, Neoplasm/genetics , Female , Humans , Loss of Heterozygosity , Male , Melanoma/pathology , Microsatellite Repeats , Middle Aged , Neoplasm MetastasisSubject(s)
Biomarkers, Tumor/analysis , Melanoma/genetics , Sarcoma, Kaposi/pathology , Skin Neoplasms/genetics , Adult , Aged , Aged, 80 and over , Biomarkers, Tumor/genetics , Blotting, Southern , Female , Humans , Immunohistochemistry , Male , Melanoma/chemistry , Middle Aged , Prognosis , RNA, Messenger/analysis , Reverse Transcriptase Polymerase Chain Reaction , Skin Neoplasms/chemistryABSTRACT
SYBL1 is a gene in the 320kb human pseudo-autosomal region at the terminus of Xq and Yq. In contrast to other pseudoautosomal genes, SYBL1 is inactivated on one X in every female cell, and is also inactive on the Y of male cells. Hypermethylation of the CpG island associated with the human gene is involved in this phenomenon. In an attempt to further examine its regulation, the genomic organization of the X-linked mouse Sybl1 homolog was analyzed and compared with the human gene. Human and mouse show the same exon number, exon-intron junctions and a highly conserved basal promoter. The structural and functional conservation of basal regulatory regions suggests that inactivation is imposed by similar auxiliary epistatic regulatory mechanism.
Subject(s)
Genes/genetics , Membrane Proteins/genetics , Animals , Base Sequence , Binding Sites , Blotting, Northern , Chloramphenicol O-Acetyltransferase/genetics , Chloramphenicol O-Acetyltransferase/metabolism , DNA/chemistry , DNA/genetics , Exons , Gene Expression , Gene Expression Regulation, Developmental , HeLa Cells , Humans , Introns , Male , Mice , Molecular Sequence Data , Promoter Regions, Genetic/genetics , R-SNARE Proteins , RNA, Messenger/genetics , RNA, Messenger/metabolism , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Regulatory Sequences, Nucleic Acid/genetics , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Tissue Distribution , Transcription, GeneticABSTRACT
The RPGR (retinitis pigmentosa GTPase regulator) gene has been shown to be mutated in 10-20% of patients with X-linked retinitis pigmentosa (XLRP), a severe form of inherited progressive retinal degeneration. A total of 29 different RPGR mutations have been identified in northern European and United States patients. We have performed mutation analysis of the RPGR gene in a cohort of 49 southern European males affected with XLRP. By multiplex SSCA and automatic direct sequencing of all 19 RPGR exons, seven different and novel mutations were identified in eight of the 49 families; these include three splice site mutations, two microdeletions, and two missense mutations. RNA analysis showed that the three splice site defects resulted in the generation of aberrant RPGR transcripts. Six of these mutations were detected in the conserved amino-terminal region of RPGR protein, containing tandem repeats homologous to the RCC1 protein, a guanine nucleotide-exchange factor for Ran-GTPase. Several exonic and intronic sequence variations were also detected. None of the RPGR mutations reported in other populations were identified in our series. Our results are consistent with the notions of heterogeneity and minority causation of XLRP by mutations in RPGR in Caucasian populations.
Subject(s)
Carrier Proteins/genetics , Eye Proteins , Genetic Linkage , Mutation , Retinitis Pigmentosa/genetics , X Chromosome , Base Sequence , DNA Mutational Analysis , Europe/epidemiology , Exons , Female , Gene Deletion , Genetic Variation , Humans , Introns , Male , Molecular Sequence Data , Mutation, Missense , Pedigree , Polymorphism, Genetic , RNA Splicing , Retinitis Pigmentosa/diagnosis , Reverse Transcriptase Polymerase Chain Reaction , United States/epidemiologyABSTRACT
PURPOSE: Reverse transcriptase (RT) polymerase chain reaction (PCR) with multiple markers has been demonstrated to be highly sensitive in detecting circulating cells from patients with malignant melanoma (MM). We evaluated the clinical significance of the presence in peripheral blood of specific PCR-positive mRNA markers as an expression of circulating melanoma cells. PATIENTS AND METHODS: Total cellular RNA was obtained from the peripheral blood of 235 patients with either localized (n = 154) or metastatic (n = 81) melanoma. We performed RT-PCR using tyrosinase, p97, MUC18, and MelanA/MART1 as gene markers. The PCR products were analyzed by gel electrophoresis and Southern blot hybridization. In addition, 20 healthy subjects and 21 patients with nonmelanoma cancer were used as negative controls. RESULTS: Although detected at various levels among assessable patients, each mRNA marker was significantly correlated with disease stage. A significant correlation with disease stage was demonstrated for patients who were positive to all four markers (P < .0001) or to at least three markers (P < .001). Univariate analysis showed a significant correlation between risk of recurrence (evaluated in stage I, II, and III patients) and increasing number of PCR-positive markers (P = .0002). Logistic regression multivariate analysis indicated that each single marker (except tyrosinase) and, more especially, the presence of four PCR-positive markers remained statistically independent prognostic factors for tumor progression. CONCLUSION: Our data establish the existence of a significant correlation among clinical stages, tumor progression, and presence of circulating melanoma-associated antigens in peripheral blood of MM patients. Preliminary assessment of a subset of patients with a higher risk of recurrence needs longer follow-up and further studies to define the role of RT-PCR in monitoring MM patients.
Subject(s)
Antigens, CD , Melanoma/pathology , Neoplastic Cells, Circulating , Neural Cell Adhesion Molecules , Polymerase Chain Reaction , Skin Neoplasms/pathology , Adult , Aged , Aged, 80 and over , Antigens, Neoplasm , Biomarkers, Tumor/blood , CD146 Antigen , Disease Progression , Female , Humans , MART-1 Antigen , Male , Melanoma/immunology , Melanoma/secondary , Melanoma-Specific Antigens , Membrane Glycoproteins/analysis , Membrane Glycoproteins/genetics , Middle Aged , Monophenol Monooxygenase/analysis , Monophenol Monooxygenase/genetics , Neoplasm Proteins/analysis , Neoplasm Proteins/blood , Neoplasm Proteins/genetics , RNA, Messenger/blood , RNA-Directed DNA Polymerase , Sensitivity and Specificity , Skin Neoplasms/immunologyABSTRACT
Methylation of CpG islands is an established transcriptional repressive mechanism and is a feature of silencing in X chromosome inactivation. Housekeeping genes that are subject to X inactivation exhibit differential methylation of their CpG islands such that the inactive alleles are hypermethylated. In this report, we examine two contrasting X-linked genes with CpG islands for regulation by DNA methylation: SYBL1, a housekeeping gene in the Xq pseudoautosomal region, and GPC3, a tissue-specific gene in Xq26 that is implicated in the etiology of the Simpson-Golabi-Behmel overgrowth syndrome. We observed that in vitro methylation of either the SYBL1 or the GPC3 promoter resulted in repression of reporter constructs. In normal contexts, we found that both the Y and inactive X alleles of SYBL1 are repressed and hypermethylated, whereas the active X allele is expressed and unmethylated. Furthermore, the Y and inactive X alleles of SYBL1 were derepressed by treatment with the demethylating agent azadeoxycytidine. GPC3 is also subject to X inactivation, and the active X allele is unmethylated in nonexpressing leukocytes as well as in an expressing cell line, suggesting that methylation is not involved in the tissue-specific repression of this allele. The inactive X allele, however, is hypermethylated in leukocytes, presumably reflecting early X inactivation events that become important for gene dosage in expressing lineages. These and other data suggest that all CpG islands on Xq, including the pseudoautosomal region, are subject to X inactivation-induced methylation. Additionally, methylation of SYBL1 on Yq may derive from a process related to X inactivation that targets large chromatin domains for transcriptional repression.
Subject(s)
DNA Methylation , Gene Expression Regulation/genetics , Heparan Sulfate Proteoglycans , Heparitin Sulfate/genetics , Membrane Proteins/genetics , Proteoglycans/genetics , Transcription, Genetic/genetics , X Chromosome/genetics , Aza Compounds/pharmacology , Cell Line , CpG Islands/genetics , Deoxycytidine/analogs & derivatives , Dosage Compensation, Genetic , Glypicans , Leukocytes/metabolism , Molecular Sequence Data , Polymerase Chain Reaction/methods , Promoter Regions, Genetic/genetics , R-SNARE Proteins , Sequence Analysis, DNA , Transfection/genetics , Y Chromosome/geneticsABSTRACT
ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We performed Northern blot experiments using sequences from ERV9-LTR, and we observed a different pattern of expression in several different hemopoietic tumor cell lines. It is possible that by the result of a somatic integration event, or by virtue of their original dispersal in the genome, ERV9-LTRs may specifically induce the expression of different cellular sequences in different cell lineages. Here, we describe the identification and analysis of four chimeric cDNA clones isolated from the T-lymphoma Peer cell line, having a structure consistent with transcription initiation from an ERV9-LTR. All the cDNA clones represent transcripts derived from unique cellular sequences. We also report the genomic localization of these cDNA clones.
Subject(s)
Genes, Regulator/genetics , Genes, Viral , Repetitive Sequences, Nucleic Acid , Retroviridae/genetics , Base Sequence , Chimera , Chromosome Mapping , Cloning, Molecular , DNA, Complementary , Gene Expression Regulation , Gene Library , Humans , Molecular Sequence Data , Transcription, Genetic/genetics , Tumor Cells, CulturedABSTRACT
Charcot-Marie-Tooth type I demyelinating neuropathies are genetically heterogeneous disorders (chrmosome 17,1,X). There are at least three genes on X chromosome, the more frequently involved being Cx32 in Xq13.1. Cx32 encodes for connexin-32, a gap junction protein of 283 aminoacids. We report the results of molecular studies in a CMTX1 Italian family, in which the mutation, found in the 5'-UTR, resulted in an abnormal mRNA connexin-32 expression. Mutations in PMP22 and P0 genes were also excluded in this family. Cx32 gene analysis carried out by PCR-SSCP on family members genomic DNAs, running a 321 bp fragment spanning the TATA box, the trasciptional start site, and the non coding exon 1b, revealed a shift correlated with a transition from C to T at position 40 of exon 1b of the 12 affected members, while was not found in the controls. Then the RT PCR-SSCP on cDNA from two peripheral nerve biopsies of two heterozygous females of the family were sequenced showing only the wild-type alleles and suggesting that mutated mRNAs were too unstable to be detected. The result also suggests a regulating role of the 5'-UTR of Cx32 mRNA.
Subject(s)
5' Untranslated Regions/genetics , Charcot-Marie-Tooth Disease/genetics , Connexins/genetics , Mutation/genetics , RNA, Messenger/genetics , X Chromosome/genetics , Female , Humans , RNA, Messenger/biosynthesis , Gap Junction beta-1 ProteinSubject(s)
Calcium Channels/genetics , Chromosome Mapping , Chromosomes, Human, Pair 11 , Teratocarcinoma/genetics , DNA, Complementary , DNA, Neoplasm/analysis , Gene Expression Regulation, Neoplastic , Gene Library , Humans , In Situ Hybridization, Fluorescence , Laurence-Moon Syndrome/genetics , Retinal Degeneration/genetics , TRPC Cation Channels , TRPC6 Cation Channel , Transcription, Genetic , Vision, Ocular/geneticsABSTRACT
The recently discovered second pseudoautosomal region (XqPAR) contains at least two genes, IL9R and SYBL1. Recent findings show that, like XpPAR genes, IL9R escapes X inactivation and its Y allele is also expressed, but SYBL1 seems to act like an X-linked gene, expressed from the active X chromosome but not from the inactive X or Y. Here we show that differences are also seen in the evolution of the sex chromosome locations of IL9R and SYBL1. IL9R is known to be autosomal in mice, and is X-linked only in primates. SYBL1, however, has been found to be on the X chromosome in all mammals tested, from marsupials to humans. Both genes were duplicated on the Y homologue of the terminal portion of the X chromosome during the evolution of Homo sapiens from other higher primates. The inactivation pattern of SYBL1 may be correlated with its longer history of X linkage, and at a more centromeric chromosomal position during evolution; the more recent X linkage and more telomeric position of the IL9R gene may explain its autosomal, 'uninactivated' transcriptional status.
Subject(s)
Evolution, Molecular , Gene Expression Regulation , Membrane Proteins/genetics , Receptors, Interleukin/genetics , X Chromosome , Animals , Base Sequence , Chromosome Mapping , DNA, Complementary , Genome , Humans , Marsupialia/genetics , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Primates/genetics , R-SNARE Proteins , Receptors, Interleukin-9ABSTRACT
The p16INK4 tumor-suppressor gene (also known as CDKN2, CDK41 and MTS1) encodes a negative regulator of the cell cycle. This gene, located in 9p21, is mutated or homozygously deleted in a high percentage of tumor cell lines and specific types of primary tumors. We have examined the status of the p16INK4 gene in 31 thyroid tumors and 7 thyroid cell lines. No DNA abnormalities were found in primary tumors. Conversely, p16INK4 gene structural alterations, deletions and point mutations were found in 4 thyroid cell lines. The expression of the 2 different p16INK4 mRNAs, the p16alpha and p16beta transcripts, was determined by RNA-PCR experiments. All the primary thyroid tumors expressed the beta transcript, while the p16alpha was barely detectable. The thyroid cell lines always expressed the p16beta transcript, while the alpha transcript was absent or, whenever present, coded for a mutated form of the p16INK4 gene product. Taken together, our results suggest that loss of p16INK4 function is not directly involved in the process of thyroid-tumor development, but it probably gives cells in tissue culture a selective growth advantage.
Subject(s)
Carrier Proteins/genetics , Cell Cycle Proteins , Genes, Tumor Suppressor , Thyroid Neoplasms/genetics , Tumor Suppressor Proteins , Amino Acid Sequence , Base Sequence , Carrier Proteins/analysis , Cyclin-Dependent Kinase Inhibitor p15 , Cyclin-Dependent Kinase Inhibitor p16 , Humans , Molecular Sequence Data , Mutation , Polymerase Chain Reaction , RNA, Messenger/analysis , Tumor Cells, CulturedABSTRACT
ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We report here the structural organization in different primate species of a zinc-finger coding gene whose expression is driven in humans by a solitary ERV9-LTR promoter. Using a PCR strategy and library screening, we were able to trace the origin of the insertion event in the primate lineage and to evaluate the impact of this event on gene structure. Our findings indicate that the integration of the ERV9 element occurred after the split of orangutang from the great apes, but before the divergence of the gorilla lineage. These results suggest that ERV9 elements have been mobile within the primate lineages and may still be active in humans.
Subject(s)
Biological Evolution , Genes, Regulator/genetics , Primates/genetics , Retroviridae/genetics , Zinc Fingers/genetics , Amino Acid Sequence , Animals , Base Sequence , Chlorocebus aethiops , DNA, Viral , Gorilla gorilla , Humans , Macaca mulatta , Molecular Sequence Data , Pan troglodytes , Polymerase Chain Reaction , Pongo pygmaeus , Repetitive Sequences, Nucleic Acid/geneticsABSTRACT
ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We describe here the structure of the ZNF80 cDNA clone putatively coding for a zinc-finger protein, whose 5' terminus starts from within an ERV9-LTR. Characterization of the single copy genomic locus indicates that a complete ERV9-LTR element is present upstream of the ZNF80 coding region and that this element acts as a functional promoter in both in vivo and in vitro experiments. A 2.6 kb long transcript is selectively expressed only in some hematopoietic cell lineages. Interestingly we mapped the ZNF80 locus to the 3q13.3 band, a region involved in karyotype rearrangements associated with myelocytic disorders. We have also analyzed the ZNF80 genomic organization in African green monkey and we show that this lower primate does not harbour an ERV9 element at this locus. Our findings strongly suggest that the expression of a zinc finger gene, which is highly conserved during evolution of primates, is regulated in humans by an LTR element of the ERV9 family.
Subject(s)
DNA-Binding Proteins/genetics , Gene Expression Regulation, Viral/genetics , Repetitive Sequences, Nucleic Acid/genetics , Retroviridae/genetics , Zinc Fingers/genetics , Animals , Base Sequence , Cells, Cultured , Chlorocebus aethiops , Chromosome Mapping , Chromosomes, Human, Pair 3 , Cloning, Molecular , DNA-Binding Proteins/chemistry , Genes/genetics , Humans , Kruppel-Like Transcription Factors , Molecular Sequence Data , Promoter Regions, Genetic/genetics , RNA, Messenger/biosynthesis , Sequence Analysis, DNA , Sequence Homology, Nucleic AcidABSTRACT
ERV9 is a low repeated family of human endogenous retroviral elements whose expression is mainly detectable in undifferentiated embryonal carcinoma NT2/D1 cells. To define all the elements required for the correct transcription activity of the ERV9 promoter and to establish a precise correlation between the elements important for basal transcription, we have systematically analyzed the in vivo and in vitro transcriptional activity of many different ERV9 promoter mutants, including a series of linker-scanning mutations across the promoter region. We report here that the ERV9 promoter contains two elements controlling the selection of the correct start sites, a TATA box and an Inr-like region; the concerted action of both elements is necessary for faithful transcription. Finally, using a series of GAL4 protein fusion constructs in cotransfection experiments, we demonstrated that various transcription factors can synergistically induce a high level of transcription when bound to an ERV9 DNA promoter.
Subject(s)
Genome, Human , Promoter Regions, Genetic/genetics , Proviruses/genetics , Retroviridae/genetics , Transcription, Genetic , Base Sequence , Carcinoma, Embryonal , DNA Mutational Analysis , Humans , Molecular Sequence Data , Sequence Homology, Nucleic Acid , Transcription Factors/pharmacology , Transcriptional Activation/drug effects , Tumor Cells, CulturedABSTRACT
The human genome contains a variety of genetic elements similar in structure to retroviruses and retrotransposons. We report here the structural and functional organization of a novel human endogenous retroviral family (ERV9). Three polyadenylated RNAs, 8, 2, and 1.5 kb long, are detected by Northern blot in undifferentiated embryonal carcinoma NT2/D1 cells. Upon genomic cloning of an expressed ERV9 locus, we demonstrated that the three polyadenylated RNAs are originated by a single ERV9 locus by alternative usage of splicing and polyadenylation signals. DNA sequence analysis of different ERV9 LTRs have revealed that they are heterogeneous in length and that the length variability is due to the number of tandemly repeated subelements present in both U3 and U5 regions; moreover, the ERV9 LTRs are capable to drive expression of a reporter gene in transient expression assays. Finally, analysis of the ERV9 5' transcription start site has allowed us to define the U3-R-U5 organization of the ERV9 LTR.