Insights into the effect of glucose on the binding between human serum albumin and the nonsteroidal anti-inflammatory drug nimesulide.

Glucose interacts with human serum albumin (HSA, the main protein responsible for the biodistribution of drugs in the bloodstream) and consequently affects the binding capacity of exogenous compounds. Thus, in this work, the interactive profile between HSA and the anti-inflammatory drug nimesulide (NMD, used mainly by patients with diabetic neuropathy to relieve acute or chronic pains) was characterized in nonglycemic, normoglycemic (80 mg/dL), and hyperglycemic (320 mg/dL) conditions by biophysics techniques. There is a spontaneous and ground-state association HSA:NMD under physiological conditions. Therefore, the Stern-Volmer constant (Ksv) can also be used to estimate the binding affinity. The Ksv values for nonglycemic, normoglycemic, and hyperglycemic conditions are around 104 M-1, indicating a moderate affinity of NMD to albumin that was slightly improved by glucose levels. Additionally, the binding is enthalpically and entropically driven mainly into subdomains IIA or IIIA. The binding perturbs weakly the α-helix content of albumin, however, glucose potentially stabilizes the tertiary structure, decreasing the structural perturbation upon NMD binding and improves the complex HSA:NMD stability. Overall, the biophysical characterization indicated that glucose levels might slightly positively impact the pharmacokinetic profile of NMD, allowing NMD to achieve its therapeutical potential without affecting drastically its effective dosages.

Effect of Co Doping on the Physical Properties and Organic Pollutant Photodegradation Efficiency of ZnO Nanoparticles for Environmental Applications.

This paper presents a comprehensive investigation of the synthesis and characterization of Zn1-xCoxO (0 ≤ x ≤ 0.05) nanopowders using a chemical co-precipitation approach. The structural, morphological, and vibrational properties of the resulting ZnO nanostructures were assessed through X-ray diffraction, scanning electronic microscopy, and Raman spectroscopy to examine the influence of cobalt doping. Remarkably, a notable congruence between the experimental results and the density functional theory (DFT) calculations for the Co-doped ZnO system was achieved. Structural analysis revealed well-crystallized hexagonal wurtzite structures across all samples. The SEM images demonstrated the formation of spherical nanoparticles in all the samples. The vibrational properties confirmed the formation of a hexagonal wurtzite structure, with an additional Raman peak corresponding to the F2g vibrational mode characteristic of the secondary phase of ZnCo2O4 observed at a 5% cobalt doping concentration. Furthermore, a theoretical examination of cobalt doping's impact on the elastic properties of ZnO demonstrated enhanced mechanical behavior, which improves stability, recyclability, and photocatalytic activity. The photocatalytic study of the synthesized compositions for methylene blue (MB) dye degradation over 100 min of UV light irradiation demonstrated that Co doping significantly improves photocatalytic degradation. The material's prolonged lifetime, reduced rate of photogenerated charge carrier recombination, and increased surface area were identified as pivotal factors accelerating the degradation process. Notably, the photocatalyst with a Zn0.99Co0.01O composition exhibited exceptional efficiency compared to that reported in the literature. It demonstrated high removal activity, achieving an efficiency of about 97% in a shorter degradation time. This study underscores the structural and photocatalytic advancements in the ZnO system, particularly at lower cobalt doping concentrations (1%). The developed photocatalyst exhibits promise for environmental applications owing to its superior photocatalytic performance.

Políticas sociais e orçamento público em um estado de exceção permanente (2013-2021) / Social policies and public budget in a state of permanent exception (2013-2021)

Resumo: Escrito sob a forma de um ensaio teórico, o artigo levanta reflexões sobre o modo de gestão do orçamento público no Brasil e sua articulação com dois eixos temáticos: tratamento da dívida pública e promoção de políticas sociais. As considerações finais apontam para a possibilidade de associar as práticas na gestão do orçamento federal brasileiro e um estado de exceção econômica perpetuado ao longo do tempo e, como desdobramento, limitante da promoção de direitos de proteção social.

Abstract: Written in the form of a theoretical essay, the article raises reflections on the way the public budget is managed in Brazil and its articulation with two thematic axes: austerity, treatment of public debt and promotion of social policies. Final considerations point to the possibility of associating practices in the management of the Brazilian federal budget and a state of economic exception perpetuated over time and, as a result, limiting the promotion of social protection rights.

Highly Selective Fluorescent Sensors: Polyethylenimine Derivatives of Triphenylamine and Coumarin for GTP and ATP Interaction via Fluorescence Lifetime Imaging Microscopy.

Chemical derivatives of polyethylenimine (PEI) receptors with either triphenylamine (TPA) or 7-hydroxy-4-methyl-coumarin (Cou) form stable complexes with adenine and guanine nucleotides in water. The host-guest complex modulation is found to be based on noncovalent molecular interactions such as π-π stacking and hydrogen bonding, which are dependent on the aromatic moieties attached to the polyaminic (PEI) backbone. PEI-TPA acts as a chemosensor with a recognition driving force based on aggregation-induced emission (AIE), involving π-π interaction between the nucleic base and TPA. It detects GTP by a chelation enhancement quenching effect of fluorescence (CHEQ) with a measured logarithm stability constant, log ß = 7.7. By varying the chemical characteristics of the fluorophore, as in the PEI-Cou system, the driving force for recognition changes from a π-π interaction to an electrostatic interaction. The coumarin derivative detects ATP with a log ß value one order of magnitude higher than that for GTP, allowing for the selective recognition of the two nucleotides in a 100% aqueous solution. Furthermore, fluorescence lifetime imaging microscopy (FLIM) allows for a correlation between the selectivity of PEI-TPA toward nucleotides and the morphology of the structures formed upon ATP and GTP recognition. This study offers valuable insights into the design of receptors for the selective recognition of nucleotides in water.

Solvent-Driven Self-Organization of Meso-Substituted Porphyrins: Morphological Analysis from Fluorescence Lifetime Imaging Microscopy.

A morphological analysis of different thin films of meso-tetra-p-(di-p-phenylamino)phenylporphyrin, H2T(TPA)4P, was made by fluorescence lifetime imaging microscopy (FLIM) and scanning electron microscopy (SEM). A comprehensive study of H2T(TPA)4P was undertaken through UV/vis absorption and fluorescence techniques in different solvents, solvent mixtures and in thin films. In solution, occurrence of intramolecular energy transfer from the triphenylamine (TPA) moieties to the porphyrin core, with quenching efficiencies in the order of 94-97%, is observed. The energy transfer rate constants are determined assuming Förster's dipole-dipole and Dexter's electron exchange mechanisms. In drop-cast-prepared thin films, from samples with different solvent mixtures, the photoluminescence (PL) quantum yield (ΦPL) decreases ∼1 order of magnitude compared to the solution behavior. FLIM and SEM experiments showed the self-organization and morphology of H2T(TPA)4P in thin films to be highly dependent on the solvent mixture used to prepare the film. In chloroform, the solvent's evaporation results in the formation of elongated and overlapped microrod structures. Introduction of a cosolvent, namely, a polar cosolvent, promotes changes in the morphology of the self-assembled structures, with the formation of three-dimensional spherical structures and hollow spheres. H2T(TPA)4P dispersed in a polymer matrix shows enhanced ΦPL values when compared to the drop-cast films. FLIM images showed coexistence of three different states or domains: aggregated, interface, and nonaggregated or less-aggregated states. This work highlights the importance of FLIM in the morphological characterization of heterogeneous films, together with the photophysical characterization of nano- and microdomains.

Effects of walking football on adherence, safety, quality of life and physical fitness in patients with prostate cancer: Findings from the PROSTATA_MOVE randomized controlled trial.

Aims: To analyze the feasibility and impact of a walking football (WF) program on quality of life (QoL), cardiorespiratory fitness (CRF), muscle strength, and balance program in men with prostate cancer under androgen deprivation therapy (ADT). Methods: Fifty patients with prostate cancer (stages IIb-IVb) under ADT were randomized to a 16-week WF program plus usual care (n=25) or usual care control group (n=25). The WF program consisted of three 90-minute sessions per week. Recruitment, withdrawal, adherence, enjoyment rate, and safety of the intervention were recorded throughout the study. Cardiorespiratory fitness was assessed before and after the interventions, while handgrip strength, lower limb muscle strength, static balance, and QoL were assessed before, during (week 8), and after (week 16) the interventions. Adverse events during sessions were also recorded. Results: The WF group showed high levels of adherence (81.6 ± 15.9%) and enjoyment rate (4.5 ± 0.5 out of 5 points). In the intention-to-treat analysis, the WF group showed an improvement in chair sit-to-stand (p=0.035) compared to the control group. Within-group comparisons showed that handgrip strength in the dominant upper limb (p=0.024), maximal isometric muscle strength in the non-dominant lower limb (p=0.006), and balance in the dominant limb (p=0.009) improved over time in the WF group but not in the usual care group. The results obtained from the per-protocol analysis indicate that CRF improved significantly in the WF group as compared to the control group (p=0.035). Within-group analysis revealed that CRF (p=0.036), muscle strength in dominant (p=0.006) and non-dominant (p=0.001) lower limbs, and balance in the non-dominant lower limb (p=0.023) improved after 16 weeks of WF, but not in the control group. One major traumatic injury (muscle tear) was reported with a complete recovery before the end of the intervention. Conclusion: This study suggests that WF is feasible, safe, and enjoyable in patients with prostate cancer under hormonal therapy. Furthermore, patients who adhere to the WF program can expect cardiorespiratory fitness, muscle strength, and balance improvements. Clinical trials registration: clinicaltrials.gov, identifier NCT04062162.

Comportamento alimentar de adultos no início da pandemia da COVID-19 / Adult eating behavior at the beginning of the COVID-19 pandemic

Introdução: Dificuldades de acesso aos alimentos e o distanciamento social desencadearam mais ansiedade, estresse e consumo de alimentos de baixo custo e ultraprocessados. Objetivo: Investigar as mudanças no comportamento alimentar e a relação deste com os aspectos socioeconômicos. Método: Estudo realizado virtualmente entre maio e junho de 2020 com 949 adultos, com questionários de dados socioeconômicos, mudanças no comportamento alimentar, frequência do consumo alimentar e o TFEQ-21. A análise foi realizada de forma descritiva e o TFEQ-21 de acordo com os escores de cada fator. A relação entre as variáveis foi analisada pelo teste de correlação de Pearson. Resultados: 63% consideraram cozinhar mais alimentos, 41% e 38% referiram maior possibilidade comer com companhia e com tranquilidade, respectivamente. Além disso, 38,6% reduziram compra de fast-food, mas 43,2% aumentaram o consumo alimentar e 41,8% o consumo de doces. Em mulheres, quanto maior o IMC, maior alimentação emocional e restrição cognitiva (p<0,001). Nestas, o comer emocional era maior se não possuíam filhos nem ensino superior (p<0,001), e a restrição era maior com a atividade física, não possuir relacionamento estável, ter filhos (p<0,001), emprego e ensino superior (p<0,05). Nos homens, o comer emocional se relacionou ao maior IMC (p<0,05) e ambas variáveis estavam relacionados à atividade física (p<0,001). Conclusão: Mudanças como cozinhar mais, reduzir fast-food, comer com tranquilidade e com companhia foram observadas comparados a antes da pandemia. Nota-se que nas mulheres há maior relação da restrição e do comer emocional com diversos aspectos do contexto social e econômico (AU).

Introduction: Difficulties in accessing food and social distancing triggered more anxiety, stress and consumption of low-cost and ultra-processed foods. Objective: To investigate changes in eating behavior and its relationship with socioeconomic aspects. Method: Study conducted virtually between May and June 2020 with 949 adults, with questionnaires on socioeconomic data, changes in eating behavior, frequency of food consumption and the TFEQ-21. The analysis was performed descriptively and the TFEQ-21 according to the scores of each factor. The relationship between the variableswas analyzed using Pearson's correlation test. Results: 63% considered cooking more food, 41% and 38% said they were more likely to eat with company and in peace, respectively. 38.6% reduced fast-food purchases, but 43.2% increased food consumption and 41.8% consumption of sweets. In women, the higher the BMI, the greater the emotional eating and cognitive restriction (p<0.001). In these, emotional eating was greater if they did not have children or higher education (p<0.001), and the restriction was greater with physical activity, not having a stable relationship, having children (p<0.001), employment and higher education (p<0.05). In men, emotional eating was related to higher BMI (p<0.05) and both variables were related to physical activity (p<0.001). Conclusion: Changes such as cooking more, reducing fast food, eating calmly and with company were observed compared to before the pandemic. It is noted that in women there is a greater relationship between restriction and emotional eating with various aspects of the social and economic context (AU).

Validation and measurement invariance of the Muscularity-Oriented Eating Test among Brazilian men and women.

OBJECTIVE: The Muscularity-Oriented Eating Test (MOET) is a 15-item unidimensional scale, designed to assess eating practices that occur in the pursuit of a muscular body. The aim of the present study was to describe the translation and cultural adaptation of the MOET to Brazilian Portuguese, to explore its factor structure and measurement invariance, and to evaluate its internal consistency, three-week test-retest reliability, and convergent validity in a community sample of Brazilian men and women. METHOD: After the back-translation procedure, the Brazilian MOET was administered online to a sample of 1246 adults (634 men and 612 women), along with measures of drive for muscularity, muscle dysmorphia symptoms, muscular/athletic-ideal internalization, disordered eating behaviors, and exercise dependence. RESULTS: Findings from an exploratory factor analysis and a confirmatory factor analysis revealed a one-factor structure and adequate internal consistency for men (ω = 0.86; α = 0.86) and women (ω = .84; α = .83). Measurement invariance across gender was supported. In addition, the scale demonstrated good three week test-retest reliability for both men (ICC = .96; p < .001) and women (ICC = .92; p < .001), and the subscales revealed moderate to large associations with drive for muscularity, muscle dysmorphia symptoms, muscular/athletic-ideal internalization, disordered eating, and exercise dependence. CONCLUSION: This study supports the validity and reliability of the MOET in a community sample of Brazilian men and women and represents an advance in measures of muscularity-oriented disordered eating in Brazilian adults, allowing for future cross-cultural studies in this field. PUBLIC SIGNIFICANCE: The Muscularity-Oriented Eating Test (MOET) is a measure of muscularity-oriented disordered eating, which assess strict adherence to diet rules, including the food's macronutrient content, regulation of protein intake, and eating less or more to influence muscle gain. This study evaluated the validity and reliability of the MOET in a community sample of Brazilian men and women. Our findings represent an advance in measures of muscularity-oriented disordered eating in Brazilian adults.

Comparison of Female Body Image Between Models and Non-Models Women / Comparação da Imagem Corporal Feminina entre Modelos de Passarela e Mulheres Não-Modelo / Comparación de la Imagen Corporal Femenina entre Modelos de Pasarela y Mujeres No Modelos

Abstract Women have been dissatisfied with their own appearance. This study aimed to evaluate whether the media can influence changes in body image regarding weight concern, satisfaction and dysfunctional investment of one's own appearance in female runway models and non-models. This survey was composed of Brazilian women, models who were linked with an agency, and non-models. Data collection was carried out using Google Forms, sending links to invite eligible subjects. In total, 102 models and 247 non-models participated in the survey. Sample Characterization Questionnaire, weight and height reports for the Body Mass Index, Sociocultural Attitudes Toward Appearance Questionnaire-3 (SATAQ-3), Multidimensional Body-Self Relations Questionnaire - Appearance Scales (MBRSQ /AS) and Appearance Schemas Inventory - Revised (ASI-R) were used. The result showed that non-models are more susceptible to pressure from the media and that having body measurements considered ideal, like models, reduces social pressure on the body.

Resumo Mulheres têm se mostrado insatisfeitas com a própria aparência. O objetivo do estudo foi avaliar se a mídia pode influenciar alterações na imagem corporal em relação à preocupação com o peso, satisfação e investimento disfuncional da própria aparência no público de mulheres modelos de passarela e não-modelos. Pesquisa utilizou brasileiras, modelos que foram vinculadas a uma agência, e não-modelos. Coleta foi realizada pelo Google Forms com envio de links convidando as participantes. Participaram da pesquisa 102 modelos e 247 não-modelos. Instrumentos utilizados foram um Questionário de Caracterização da Amostra, relato de peso/altura para o Índice de Massa Corporal, Sociocultural Attitudes Toward Appearance Questionnaire-3 (SATAQ-3), Multidimensional Body-Self Relations Questionnaire - Appearance Scales (MBRSQ/AS) e Appearance Schemas Inventory - Revised (ASI-R). Resultado demonstrou que as não-modelos estão mais suscetíveis às pressões da mídia e que ter as medidas corporais consideradas ideais, como as modelos, diminui a pressão social sobre o corpo.

Resumen Las mujeres están insatisfechas con su propria apariencia física. El objetivo de este estudio fue evaluar si los medios de comunicación pueden influir en la imagen corporal de las mujeres respecto a la preocupación por el peso, la satisfacción e inversión disfuncional de la propia apariencia en mujeres modelos de pasarela y las no modelos. Se utilizó una muestra de mujeres brasileñas, modelos vinculadas a una agencia y no modelos. Se recogieron datos de Google Forms. Se contó con la participación de 102 modelos y 247 no modelos. Se utilizaron los cuestionarios: Caracterización de Muestras, relato de peso/altura para el Índice de Masa Corporal, Sociocultural Attitudes Toward Appearance Questionnaire-3 (SATAQ-3), Multidimensional Body-Self Relations Questionnaire - Appearance Scales (MBRSQ/AS) y Appearance Schemas Inventory - Revised (ASI-R). Los resultados indicaron que las mujeres no modelos están más susceptibles a la presión de los medios de comunicación, y que tener medidas corporales consideradas ideales como las de las modelos reduce la presión social sobre el cuerpo.

Nanostructuring with Surfactants: The Self-Assembly of a New Poly(thiophene-phenylene) Conjugated Polymer Bearing Azacrown Ether Pendant Groups.

We report the synthesis of a new conjugated polymer bearing crown ether moieties, poly[(N(1-aza-[18]crown-6)carbamido)thiophene-2,5-diyl-alt-1,4-phenylene] (BG2). In water, BG2 forms a dispersion with a slightly cloudy appearance. We have studied the effect of adding surfactants, with different polar head groups, on these polymer-polymer aggregates. Special attention is given to the system with the anionic surfactant, sodium dodecyl sulfate (SDS). The combination of photophysical techniques with electrical conductivity, NMR (1H, 13C, and 27Na), DFT calculations, molecular dynamics simulations, and small-angle neutron scattering (SANS) provides a detailed picture on the behavior of the SDS/BG2 system in aqueous solution and in thin films. NMR, electric conductivity, and DFT results suggest that hydrophilic interactions occur between the polar headgroup of the surfactant (OSO3- Na+) and the aza-[18]-crown-6 moiety. DFT calculations confirmed the capability of BG2 to form stable complexes with the Na+ cations, where the cation can be either inside the azacrown cavity or sandwiched between the cavity and the polymer chain, which seem to determine the position of the surfactant hydrocarbon chain and, therefore, be responsible for the disruption of the BG2 aggregates and subsequent increase in the photoluminescence quantum yields. SANS measurements, made with hydrogenated and deuterated SDS in D2O, clearly show how micron-sized aggregates of BG2 are broken down by SDS and then how BG2 becomes preferentially incorporated within joint colloidal particles of BG2 and SDS with increasing [SDS]/[BG2] molar ratio.

Screening, Diagnosis and Management of Atrial Fibrillation in Cancer Patients: Current Evidence and Future Perspectives. / Rastreamento, Diagnóstico e Manejo da Fibrilação Atrial em Pacientes com Câncer: Evidências Atuais e Perspectivas Futuras.

Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia in the general population, carrying a high morbimortality burden, and this also holds true in cancer patients. The association between AF and cancer goes even further, with some studies suggesting that AF can be a marker of occult cancer. There is, however, a remarkable paucity of data concerning specific challenges of AF management in cancer patients. AF prompt recognition and management in this special population can lessen the arrhythmia-related morbidity and have an important prognostic benefit. This review will focus on current AF diagnosis and management challenges in cancer patients, with special emphasis on AF screening strategies and devices, and anticoagulation therapy with non-vitamin K antagonist oral anti-coagulants (NOACs) for thromboembolic prevention in these patients. Some insights concerning future perspectives for AF prevention, diagnosis, and treatment in this special population will also be addressed.

A fibrilação atrial (FA) é a arritmia cardíaca sustentada mais comum na população geral, tendo uma alta carga de morbimortalidade, e isso também é válido para pacientes com câncer. A associação entre FA e câncer vai ainda mais longe, com alguns estudos sugerindo que a FA pode ser um marcador de câncer oculto. Há, no entanto, uma notável escassez de dados sobre os desafios específicos do manejo da FA em pacientes com câncer. O reconhecimento e o manejo imediatos da FA nesta população especial podem diminuir a morbidade relacionada à arritmia e ter um importante benefício prognóstico. Esta revisão se concentrará nos desafios atuais de diagnóstico e manejo da FA em pacientes com câncer, com ênfase especial nas estratégias e dispositivos de rastreamento da FA e na terapia de anticoagulação com anticoagulantes orais não antagonistas da vitamina K (NOACs) para prevenção tromboembólica nesses pacientes. Alguns insights sobre as perspectivas futuras para a prevenção, diagnóstico e tratamento da FA nesta população especial também serão abordados.

Rastreamento, Diagnóstico e Manejo da Fibrilação Atrial em Pacientes com Câncer: Evidências Atuais e Perspectivas Futuras / Screening, Diagnosis and Management of Atrial Fibrillation in Cancer Patients: Current Evidence and Future Perspectives

Resumo A fibrilação atrial (FA) é a arritmia cardíaca sustentada mais comum na população geral, tendo uma alta carga de morbimortalidade, e isso também é válido para pacientes com câncer. A associação entre FA e câncer vai ainda mais longe, com alguns estudos sugerindo que a FA pode ser um marcador de câncer oculto. Há, no entanto, uma notável escassez de dados sobre os desafios específicos do manejo da FA em pacientes com câncer. O reconhecimento e o manejo imediatos da FA nesta população especial podem diminuir a morbidade relacionada à arritmia e ter um importante benefício prognóstico. Esta revisão se concentrará nos desafios atuais de diagnóstico e manejo da FA em pacientes com câncer, com ênfase especial nas estratégias e dispositivos de rastreamento da FA e na terapia de anticoagulação com anticoagulantes orais não antagonistas da vitamina K (NOACs) para prevenção tromboembólica nesses pacientes. Alguns insights sobre as perspectivas futuras para a prevenção, diagnóstico e tratamento da FA nesta população especial também serão abordados.

Abstract Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia in the general population, carrying a high morbimortality burden, and this also holds true in cancer patients. The association between AF and cancer goes even further, with some studies suggesting that AF can be a marker of occult cancer. There is, however, a remarkable paucity of data concerning specific challenges of AF management in cancer patients. AF prompt recognition and management in this special population can lessen the arrhythmia-related morbidity and have an important prognostic benefit. This review will focus on current AF diagnosis and management challenges in cancer patients, with special emphasis on AF screening strategies and devices, and anticoagulation therapy with non-vitamin K antagonist oral anti-coagulants (NOACs) for thromboembolic prevention in these patients. Some insights concerning future perspectives for AF prevention, diagnosis, and treatment in this special population will also be addressed.

Validation of the Muscle Dysmorphic Disorder Inventory (MDDI) in Brazilian Women.

Despite high levels of body dissatisfaction and an increasing drive for muscularity among Brazilian women, most of the existing literature on muscle dysmorphia focuses on men and has mainly been conducted in Western and English-speaking regions. As a result, one of the most widely used assessment tools for symptoms of the disorder, the Muscle Dysmorphic Disorder Inventory (MDDI), has not yet been evaluated in Brazilian women-an at-risk population. In the present study, we perform a psychometric evaluation of the Brazilian Portuguese translation of the MDDI in a sample of 515 women. We evaluated the factor structure using a two-step, split-sample exploratory and confirmatory factor analytic approach, which supported the original three-factor structure of the measure. Additionally, we found good internal consistency, convergent validity, and test-retest reliability of the measure. Collectively, these results support the use of the measure in Brazilian women and provide a foundation to expand the literature in this population.

Complexes of In(III) with 8-hydroxyquinoline-5-sulfonate in solution: structural studies and the effect of cationic surfactants on the photophysical behaviour.

Following previous studies on the complexation in aqueous solutions of 8-hydroxyquinoline-5-sulfonate (8-HQS) with the trivalent metal ions, Al(III) and Ga(III) and various other metal ions, using multinuclear NMR, DFT calculations, UV-vis absorption and luminescence techniques, we have extended our studies on 8-HQS complexation to the trivalent metal ion In(III). The study combines the high sensitivity of luminescence techniques and the selectivity of multinuclear NMR spectroscopy with the structural details accessible through DFT calculations, and aims to obtain a complete understanding of the complexation between the In3+ metal ion and 8-HQS, and how this influences the luminescence behaviour. A full speciation study has been performed and, as has been reported for the complexes of 8-hydroxyquinoline (8-HQ), the dominant complexes of 8-HQS with In(III) show marked differences in the complexation behaviour when compared with the equivalent complexes with the other group 13 cations Al(III) and Ga(III). While all three complexes have a 1 : 3 (metal : ligand) stoichiometry, those with Al(III) and Ga(III) show a mer-geometry of the ligands around the metal centre, whereas the fac-geometry is observed for the complexes with In(III). On binding to metal ions, 8-HQS shows a marked increase in the intensity of the fluorescence emission band compared to that of the virtually non-luminescent free ligand. However, the increase for In(III) is less pronounced than with Al(III) or Ga(III). These observations have important implications for the application of the complexes in sensing, light emitting devices (e.g. OLEDs), or as electron transport layers in photovoltaics for solar energy conversion. Furthermore, surfactant complexation is known to improve the fluorescence intensity in metal complexes with 8-HQS, by inhibiting the ligand exchange, as we have reported for complexes of HQS with Al(III) and Ga(III). Accordingly, in view of the development of applications in either sensing or optoelectronics, our interest also includes the study of HQS complexes of In(III) in the presence of cationic surfactants, in comparison with previous results with Al(III) and Ga(III).

Long-term response to avelumab and management of oligoprogression in Merkel cell carcinoma: A case report.

BACKGROUND: Merkel cell carcinoma (MCC) is a rare and aggressive cutaneous neuroendocrine neoplasia, with high risk of recurrence and metastasis and poor survival. Immune checkpoint inhibitors, like the anti-programmed death-ligand 1 agent avelumab, were recently approved for the treatment of advanced MCC. We, herein, report the first case of advanced MCC with oligoprogression managed with avelumab and local radical treatment. CASE SUMMARY: A 61-year-old man was presented to the hospital with sporadic fever and an exudative malodorous mass (10 cm of diameter), located on the right gluteal region. The final diagnosis was MCC, cT4N3M1c (AJCC, TNM staging 8th edition, 2017), with invasion of adjacent muscle, in-transit metastasis, and bone lesions. Patient started chemotherapy (cisplatin and etoposide), and after six cycles, the main tumor increased, evidencing disease progression. Two months later, the patient started second line treatment with avelumab (under an early access program). After two cycles of treatment, the lesion started to decrease, achieving a major response. Local progression was documented after 16 cycles. However, as the tumor became resectable, salvage surgery was performed, while keeping the systemic treatment with avelumab. Since the patient developed bilateral pneumonia, immunotherapy was suspended. More than 2.5 years after surgery (last 19 mo without systemic therapy), the patient maintains complete local response and stable bone lesions. CONCLUSION: This report highlights the efficacy and long-term response of avelumab on the management of a chemotherapy resistant advanced MCC, with evidence of oligoprogression, in combination with local radical treatment.

Family influence on the nutritional status and eating habits of six to nine year-old children / Influência familiar no estado nutricional e hábito alimentar de crianças de seis a nove anos

ABSTRACT Objective To investigate whether caregivers' attitudes, beliefs, practices, alimentary habits, and nutritional status influence the alimentary habits and nutritional status of children aged six to nine years. Methods This cross-sectional study included 164 children and a family member (caregiver) each (n=164), carried out in the family health units of the municipality of Ribeirão Preto (SP), Brazil. Weight and height measurements were performed; each child was then evaluated by the application of both the Child Feeding Questionnaire and 24h recall (for calculating the Healthy Eating Index Revised), in addition to the assessment of adherence to healthy eating steps of the Ministry of Health, through a questionnaire. Results The results showed that the prevalence of being overweight was 18.3% in children and 32.9% in caregivers, and the prevalence of obesity was 15.9% and 37.9%, respectively. Most of the 56 children categorized as overweight also had overweight caregivers (82.1%; n=46). Concerns regarding children's weight control were higher among caregivers responsible for overweight children (3.6±1.29). In contrast, caregivers responsible for children below or at normal weight demonstrated a greater tendency toward getting children to eat (3.3±0.97 and 3.9±0.99, respectively). The average score; of the children's Brazilian Healthy Eating Index Revised (50.0±13.6) was similar to that of their caregivers (56.5±12.1). Conclusions Caregivers have a direct influence on the nutritional status and eating habits of children; therefore, they should be targeted in the processes of nutritional intervention for the prevention and treatment of childhood obesity.

RESUMO Objetivo Este estudo se propôs a investigar se atitudes, crenças, práticas, hábitos alimentares e estado nutricional dos responsáveis influenciam os hábitos alimentares e o estado nutricional de crianças de 6 a 9 anos. Methods Estudo transversal com 164 crianças e um respectivo responsável por sua alimentação (n=164), desenvolvido em Unidades de Saúde da Família do município de Ribeirão Preto (SP). Realizaram-se aferição de peso e estatura, aplicação do Questionário de Alimentação da Criança e Recordatório Alimentar de 24 horas (para cálculo do Índice de Qualidade da Dieta Revisado), além de verificação da adesão aos Passos da Alimentação Saudável do Ministério da Saúde, por meio de um questionário. Results Os resultados obtidos mostraram que as prevalências de sobrepeso foram de 18,3% nas crianças e de 32,9% nos responsáveis, e as prevalências de obesidade foram de 15,9% e 37,9%, respectivamente. Os responsáveis da maioria das 56 crianças que apresentaram excesso de peso também tinham excesso de peso (82,1%; n=46). A preocupação com o peso da criança foi maior entre os responsáveis das crianças acima do peso (3,6±1,29). Por outro lado, uma maior pressão para comer foi identificada entre os responsáveis com crianças com baixo peso ou peso normal (3,3±0,97 e 3,9±0,99, respectivamente). O escore médio do Índice de Qualidade da Dieta Revisado das crianças (50,0±13,6) foi muito similar ao dos responsáveis (56,5±12,1). Conclusions Os responsáveis exercem influência direta no estado nutricional e hábitos alimentares das crianças, devendo ser alvos nos processos de intervenção nutricional para a prevenção e o tratamento da obesidade infantil.

A Randomized Crossover Intervention Study on the Effect a Standardized Maté Extract (Ilex paraguariensis A. St.-Hil.) in Men Predisposed to Cardiovascular Risk.

(1) Background: Due to its richness in chlorogenic acids (CGAs), Maté (Ilex paraguariensis A. St.-Hil.) could be of interest in the prevention of cardiometabolic diseases, however clinical evidence are lacking. This trial aimed to evaluate the impact of maté CGAs, consumed in a daily dose achievable through traditional maté beverages, on parameters related to cardiometabolic risk. (2) Design: Thirty-four male volunteers aged 45-65 years and with at most one criteria of metabolic syndrome, were recruited for a randomized, double-blind, placebo-controlled, and crossover study. The volunteers were assigned to consume an encapsulated dry maté extract for four-weeks, providing 580 mg of caffeoyl quinic acid derivatives (CQAs) daily, or a placebo, with a two weeks washout between intervention periods. Anthropometric variables, blood pressure, plasma glucose, lipids, endothelial, and inflammatory biomarkers were measured in overnight-fasted subjects and after a glucose load. (3) Results: We found no significant effects of treatment on these parameters and the response to the glucose load was also similar between the two interventions. However, a significant decrease in fasting glucose was observed between day 0 and day 28 for the maté group only (-0.57 ± 0.11 mmol/L, p < 0.0002). In subjects with an intermediate to high Framingham risk score, consumption of maté extract induced a 10% increase of high-density lipoprotein (HDL)-c from baseline. In a subgroup representative of the study population, significant decreases in the C-reactive protein (CRP) (-50%) and interleukin-6 (IL-6) (-19%) levels were observed. (4) Conclusions: These clinical observations suggest that maté, naturally rich in CGAs, could improve some cardiometabolic markers in subjects with a higher predisposition to metabolic syndrome, even if that remains to be confirmed in new trials specifically targeting this population.

Photophysical studies on lanthanide(III) chelates conjugated to Pittsburgh compound B as luminescent probes targeted to Aß amyloid aggregates.

The photophysical properties of Eu3+ and Tb3+ complexes of DOTAGA and DO3A-monoamide conjugates of the Pittsburgh compound B (PiB) chromophore, prepared using linkers of different lengths and flexibilities, and which form stable negatively charged (LnL1), and uncharged (LnL2) complexes, respectively, were studied as potential probes for optical detection of amyloid aggregates. The phenylbenzothiazole (PiB) moiety absorbs light at wavelengths longer than 330 nm with a high molar absorption coefficient in both probes, and acts as an antenna in these systems. The presence of the luminescent Ln3+ ion quenches the excited states of PiB through an energy transfer process from the triplet state of PiB to the metal centre, and structured emission is seen from Eu3+ and Tb3+. The luminescence study indicates the presence of a 5D4 → T1 back transfer process in the Tb3+ complexes. It also provides insights on structural properties of the Eu3+ complexes, such as the high symmetry environment of the Eu3+ ion in a single macrocyclic conformation and the presence of one water molecule in its inner coordination sphere. The overall quantum yield of luminescence of EuL1 is higher than for EuL2. However, their low values reflect the low overall sensitization efficiency of the energy transfer process, which is a consequence of the large distances between the metal center and the antenna, especially in the EuL2 complex. DFT calculations confirmed that the most stable conformation of the Eu3+ complexes involves a combination of a square antiprismatic (SAP) geometry of the chelate and an extended conformation of the linker. The large calculated average distances between the metal center and the antenna point to the predominance of the Förster energy transfer mechanism, especially for EuL2. This study provides insights into the behavior of amyloid-targeted Ln3+ complexes as optical probes, and contributes towards their rational design.

Association between creatine kinase activity, oxidative stress and selenoproteins mRNA expression changes after Brazil nut consumption of patients using statins.

BACKGROUND & AIMS: Although the mechanisms by which statins promote muscle disorders remain unclear, supplementation with dietary antioxidants may mitigate statins' side effects. This study aimed to investigate whether the consumption of Brazil nuts modulates serum creatine kinase (CK) activity in patients regularly using statins. METHODS: The study was performed in the Ribeirão Preto Medical School University Hospital. Thirty-two patients in regular use of statins were divided according to CK activity levels (G1: increased or G2: normal) and received one unit of Brazil nut daily for 3 months. Body composition, blood selenium (Se) concentrations, erythrocyte glutathione peroxidase (GPX) activity, oxidative stress parameters, and CK activity were evaluated before and after supplementation. RESULTS: In both groups, supplementation with one Brazil nut daily for 3 months contributed to achieve decreased levels of CK activity in serum, with positive changes in plasma and erythrocyte Se concentrations (p < 0.0001), and increased levels of GPX activity. Among the parameters related to curbing of oxidative stress, we observed reduced levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in both groups after supplementation. We also found a moderately negative association between CK and GPX activity (r = -41; p < 0.02). Expression of selenoproteins GPX1, SELENOP, and SELENON after Brazil nut supplementation was unchanged. CONCLUSION: Brazil nut consumption enhanced the control of CK activity by improving oxidative stress biomarkers in patients using statins but did not modulate mRNA expression of selenoproteins.