Adeno-associated virus mediated gene delivery of Perm1 enhances cardiac contractility in mice.

Reduced muscle contractility and mitochondrial bioenergetics are the hallmarks of systolic heart failure. There is currently no therapy targeting both. Here, we show that gene delivery of Perm1 via adeno-associated virus (AAV) simultaneously enhances cardiac contractility and mitochondrial biogenesis in C57BL6 mice. Moreover, we found that PERM1 interacts with Troponin C (TnC), a key contractile protein in striated muscle, and that AAV-Perm1 led to upregulation of TnC. This study suggests that gene delivery of Perm1 may be a novel therapeutic approach to treat systolic heart failure by simultaneously restoring cardiac contractility and mitochondrial bioenergetics.

Ghanaian clients' perception of fitness instructors' adherence to exercise delivery services codes of conduct: An exploratory study.

Background and Aims: Patronage for fitness and wellness activities has increased in Ghana, but the perception of primary consumers regarding exercise delivery service codes of conduct (EDCC) remains undocumented. This study reported the perception of clients about fitness instructors' adherence to EDCC. Methods: Three hundred and seventy-nine (mean age = 26.12 ± 8.83 years) clients registered with National Sports for ALL Association, Ghana (NASFAAG) were recruited. The participants were not living with any diagnosed disability, using psychiatric medication, and had consistently participated in fitness training, at least three times a week for a year, and not below the age of 18 years. The participants were recruited from fitness and wellness centers, gyms, and fitness clubs in three regions (Greater Accra-GA, Upper East-UE, and Ashanti-A) of Ghana. A closed-ended, self-structured and validated awareness and adherence exercise delivery codes of conduct questionnaires was administered. The questionnaire focused on data protection and responsibility, informed consent, competence, and professional and personal conduct adapted from the British Association of Sports and Exercise Science codes of conduct was administered. Statistical Package for Social Sciences (SPSS) version 23.0 was used to run factor analysis which determined factorial distribution of clients' perception of instructors on codes of conduct. Results: In total, 50.99% (UE), 47.68% (A), and 46.02% (GA) clients indicated that identities were unprotected when trainers displayed information. In all, 31.05% (UE), 40.34% (A), and 36.48% (GA) showed they were introduced to substances without consent. In total, 38.89% (UE), 32.70% (A), and 53.55% (GA) clients participated in training to realize that the expertise expected was not provided. In all, 38.10% (UE) and 36.23% (A) agreed that instructors put safety at risk, while 23.02% (GA) exploited clients for personal gain. Conclusion: Fitness instructors need enlightenment to adhere ethically to EDCC activities in Ghana. Activities related to wellness and fitness in Ghana require regulations.

Physiological, anthropometric parameters, and balance skill response of healthy bankers to fitness training.

Sedentary lifestyle as a predisposing factor of chronic diseases like hypertension, diabetes, stroke and obesity is a common phenomenon in the banking job. Studies suggest that fitness training improves health of bankers but has not been established among Ghanaian bankers. This study examined the physiological, anthropometric parameters, and balance skill responses of relatively healthy bankers to fitness training. Twelve bankers aged 28 to 55 years (36.41±7.16 years) in Kumasi completed a 6-month fitness training program (FTP) of 30-min gym workouts and 1-hr swimming per session. Physiological, anthropometric parameters, and balance skill variables assessments were conducted in three trials: pretraining, midtraining and post-FTP. FTP caused significant decrease in pre-post systolic blood pressure (P=0.001), diastolic blood pressure (P=0.000), heart rate (P=0.006), waist circumference (P= 0.007), waist-to-hip ratio (P=0.007), and bone density (P=0.038). There was significance decrease in body mass index (P=0.047) between pre- and midtraining status. Weight significantly decreased among the three trials (P=0.017). Pre-post opened (P=0.043) and closed (P=0.015) eye balance skills increased significantly. Effects of FTP were significantly higher in female (P<0.05). Participants who were at the stage 1 and 2 hypertensions pretraining became normotensive posttraining. Six months FTP has beneficial effects on the physiological, anthropometric parameters, and balance skill of relatively healthy bankers.