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OBJECTIVES: To assess the impact of a patient-based self-assessed fatigue intervention aimed at promoting early postoperative ambulation. DESIGN: Prospective randomised controlled trial. SETTING: Single-centre, conducted at the Obstetrics and Gynaecology Department of the Xiangyang Central Hospital, China. PARTICIPANTS: Eligible were adult patients undergoing elective gynaecologic oncologic surgery. INTERVENTIONS: The intervention group utilised a modified Borg Rating of Perceived Experience (RPE) scale for self-assessment of fatigue levels. The control group followed fixed-activity distance guidelines postoperatively. PRIMARY AND SECONDARY OUTCOME MEASURES: The primary outcome was the self-reported the time to first flatus postoperatively. Secondary outcomes encompassed the time to first defecation, incidence of moderate-to-severe abdominal distention, ileus, postambulation adverse events (nausea, vomiting and dizziness), patient satisfaction with early ambulation instructions, compliance with early ambulation and average hospital costs and length of stay. RESULTS: Between June 2021 and October 2022, 552 patients were enrolled. The self-assessed fatigue intervention group demonstrated non-inferior the time to first flatus compared with the fixed-activity distance assessment group (25.59±14.59 hours vs 26.10±14.19 hours, pnon-inferiority<0.001). Compliance with activity was higher in the intervention group (49.40% vs 36.02%, p<0.001), although it did not reach 50%. The intervention group also exhibited significantly higher mean hospital costs, length of stay and incidence of moderate-to-severe abdominal distention (p<0.001). CONCLUSIONS: The self-assessed fatigue intervention for early postoperative ambulation in gynaecologic oncology patients shows promise as an effective strategy; however, compliance is suboptimal. An intervention based on mandatory, yet reasonable, fixed-activity distance may represent the most viable current approach. Further research is warranted to confirm these findings. TRIAL REGISTRATION NUMBER: CTR2100046035.
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Deambulação Precoce , Fadiga , Neoplasias dos Genitais Femininos , Procedimentos Cirúrgicos em Ginecologia , Complicações Pós-Operatórias , Humanos , Feminino , Fadiga/etiologia , Pessoa de Meia-Idade , Neoplasias dos Genitais Femininos/cirurgia , Estudos Prospectivos , Adulto , Idoso , Satisfação do Paciente , China , Autoavaliação (Psicologia)RESUMO
Few-atom metal clusters (FAMCs) exhibit superior performance in catalyzing complex molecular transformations due to their special spatial environments and electronic states, compared to single-atom catalysts (SACs). However, achieving the efficient and accurate synthesis of FAMCs while avoiding the formation of other species, such as nanoparticles and SACs, still remains challenges. Herein, we report a two-step strategy for synthesis of few-atom platinum (Pt) clusters by predeposition of zinc single-atom-glue (Zn1) on MgO nanosheets (Ptn-Zn1/MgO), where FAMCs can be obtained over a wide range of Pt contents (0.09 to 1.45â wt %). Zn atoms can act as Lewis acidic sites to allow electron transfer between Zn and Pt through bridging O atoms, which play a crucial role in the formation and stabilization of few-atom Pt clusters. Ptn-Zn1/MgO exhibited a high selectivity of 93 % for anti-Markovnikov alkene hydrosilylation. Moreover, an excellent activity with a turnover frequency of up to 1.6×104â h-1 can be achieved, exceeding most of the reported Pt SACs. Further theoretical studies revealed that the Pt atoms in Ptn-Zn1/MgO possess moderate steric hindrance, which enables high selectivity and activity for hydrosilylation. This work presents some guidelines for utilizing atomic-scale species to increase the synthesis efficiency and precision of FAMCs.
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The hydrazine oxidation-assisted H2 evolution method promises low-input and input-free hydrogen production. However, developing high-performance catalysts for hydrazine oxidation (HzOR) and hydrogen evolution (HER) is challenging. Here, we introduce a bifunctional electrocatalyst α-MoC/N-C/RuNSA, merging ruthenium (Ru) nanoclusters (NCs) and single atoms (SA) into cubic α-MoC nanoparticles-decorated N-doped carbon (α-MoC/N-C) nanowires, through electrodeposition. The composite showcases exceptional activity for both HzOR and HER, requiring -80â mV and -9â mV respectively to reach 10â mA cm-2. Theoretical and experimental insights confirm the importance of two Ru species for bifunctionality: NCs enhance the conductivity, and its coexistence with SA balances the H ad/desorption for HER and facilitates the initial dehydrogenation during the HzOR. In the overall hydrazine splitting (OHzS) system, α-MoC/N-C/RuNSA excels as both anode and cathode materials, achieving 10â mA cm-2 at just 64â mV. The zinc hydrazine (Zn-Hz) battery assembled with α-MoC/N-C/RuNSA cathode and Zn foil anode can exhibit 97.3 % energy efficiency, as well as temporary separation of hydrogen gas during the discharge process. Therefore, integrating Zn-Hz with OHzS system enables self-powered H2 evolution, even in hydrazine sewage. Overall, the amalgamation of NCs with SA achieves diverse catalytic activities for yielding multifold hydrogen gas through advanced cell-integrated-electrolyzer system.
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One-dimensional (1D) high-entropy compounds (HECs) with subnano diameters are highly attractive because long-range electron delocalization may occur along the high-entropy atomic chain, which results in extraordinary properties. Nevertheless, synthesizing such 1D HECs presents a substantial challenge, and the physicochemical attributes of these novel structures remain ambiguous. Herein, we developed a comelting-filling-freezing-modification (co-MFFM) method for synthesizing 1D high-entropy metal phosphide (HEP) by simultaneously encapsulating various metal cations within single-walled carbon nanotubes (SWCNTs) followed with a phosphorization process. The resulting 1D HEP nanowires confined within SWCNTs exhibit crucial features, including an ultrafine, high-entropy, and amorphous structure, along with a core-shell arrangement. The SWCNT as a shell could donate π electrons to 1D HEP for enhanced electron delocalization and protect 1D HEP as an atomically single-layered protective covering, thus boosting high electrocatalytic activity and stability. Moreover, the co-MFFM method demonstrates scalability for mass production and displays universal applicability to the synthesis of various 1D HECs.
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Airway mucous cell metaplasia and mucous hypersecretion is one of the key characteristic pathophysiological status of chronic obstructive pulmonary disease (COPD). micro(mi)RNAs are acknowledged as non-encoding RNA molecules playing important roles in gene expression regulation. In this study, we searched the Gene Expression Omnibus (GEO) database for the differentially expressed miRNAs between COPD and non-COPD controls with bioinformatics analysis. Finally, we focused on miR-513a-5p and investigated the potential mechanism by which miR-513a-5p regulates airway mucous hypersecretion and goblet cell metaplasia. A dual-luciferase reporter assay was then showing that miR-513a-5p targeted the 3'-UTR of TFR1 and inhibited its expression in vitro. In vivo transfection demonstrated that TFR1 downregulation partially blocked MUC5AC hypersecretion and goblet cell hyperplasia in COPD model rats. In vitro study, CSE increased the intracellular expression and secretion of MUC5AC by BEAS-2B branchial epithelial cells in the BEAS-2B cell and THP-1 cell coculture system. Coculture with either miR-513a-5p mimic-pretreated or TFR1-deficient THP-1 cells attenuated intracellular MUC5AC expression in BEAS-2B cells exposed to CSE. ELISA demonstrated that transfection of TFR1 siRNA or pretreatment with miR-513a-5p mimic reduced the secretion of inflammatory factors that are responsible for airway goblet cell hyperplasia, such as IL-1ß, IL-13, and IL-17, by THP-1 cells after CSE stimulation. Our findings supported that miR-513a-5p/TFR1 signaling axis might activate macrophages as well as promote airway inflammation and airway mucous cell hyperplasia in COPD.
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MicroRNAs , Doença Pulmonar Obstrutiva Crônica , Ratos , Animais , Células Caliciformes/metabolismo , Hiperplasia/genética , MicroRNAs/genética , MicroRNAs/metabolismo , Doença Pulmonar Obstrutiva Crônica/genética , Doença Pulmonar Obstrutiva Crônica/metabolismo , MetaplasiaRESUMO
The phase stability and superconductivity of lutetium polyhydrides under pressure were systematically explored via particle swarm optimization. Several lutetium hydrides, such as LuH, LuH3, LuH4, LuH6, LuH8, and LuH12, were found to be dynamically and thermodynamically stable. Combined with the electronic properties, there are a large number of H-s states and low density of Lu-f states at the Fermi level, leading to superconductivity. The phonon spectrum and electron-phonon coupling interaction are considered to calculate the superconducting critical temperature (Tc) of stable lutetium hydrides at high pressure. The new predicted cubic LuH12 has the highest Tc value of 187.2 K at 400 GPa in all the stable LuHn compounds, which was estimated by directly solving the Eliashberg equation. The calculated results provide insights into the design of new superconducting hydrides under pressure.
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Compressive hyperspectral images often suffer from various noises and artifacts, which severely degrade the imaging quality and limit subsequent applications. In this paper, we present a refinement method for compressive hyperspectral data cubes based on self-fusion of the raw data cubes, which can effectively reduce various noises and improve the spatial and spectral details of the data cubes. To verify the universality, flexibility, and extensibility of the self-fusion refinement (SFR) method, a series of specific simulations and practical experiments were conducted, and SFR processing was performed through different fusion algorithms. The visual and quantitative assessments of the results demonstrate that, in terms of noise reduction and spatial-spectral detail restoration, the SFR method generally is much better than other typical denoising methods for hyperspectral data cubes. The results also indicate that the denoising effects of SFR greatly depend on the fusion algorithm used, and SFR implemented by joint bilateral filtering (JBF) performs better than SRF by guided filtering (GF) or a Markov random field (MRF). The proposed SFR method can significantly improve the quality of a compressive hyperspectral data cube in terms of noise reduction, artifact removal, and spatial and spectral detail improvement, which will further benefit subsequent hyperspectral applications.
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This study explores the influence of organizational learning and external cooperation configuration on enterprise technological innovation and constructs a comprehensive theoretical framework of "organizational learning-external cooperation-technological innovation" based on the fuzzy set qualitative comparative analysis (fsQCA) method. The results show the following. (1) A single episode of organizational learning or external cooperation cannot affect the enterprise's technological innovation, which requires the mutual linkage of the two to improve enterprise technological innovation performance. (2) The technological innovation model in which organizational learning and external cooperation interact is an effective way for enterprises to improve technological innovation performance. There are four technological innovation models that produce high technological innovation performance, namely consciousness-system synergy, consciousness-led, quasi-full, and all-around drive. (3) There are four models of non-high-tech innovation performance, which are not opposed to the technological innovation model of high-tech innovation performance. This research expands the technological innovation perspective of organizational learning and external cooperation matching, provides enterprises with effective technological innovation activities, and provides a theoretical reference and practical guidance for improving technological innovation performance.
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Invenções , TecnologiaRESUMO
Supported single-atom catalysts (SACs), with the extremely homogenized active sites could achieve high hydrogenation selectivity toward one of the functional groups coexisting in the reactant molecule. However, as to the target group, the control of selective recognition and activation by SACs still remains a challenge. Herein, the phase engineering of the support is adopted to control the chemo-recognition behavior of SACs in selective hydrogenation. Single-atom Ru on amorphous porous ultrathin TiO2 nanosheets (Ru1/a-TiO2) is constructed, in which Ru is more positively charged than that in the crystalline counterpart (Ru1/c-TiO2). Moreover, in the nitro/vinyl selective hydrogenation process, Ru1/a-TiO2 shows superior nitro selectivity, opposite to the vinyl selectivity of Ru1/c-TiO2. Density functional theory calculations for single-atom Ru of different charge states show that the reactant adsorption configuration could be inverted in the amorphous TiO2, accounting for the chemo-recognition behavior controlled by the phase of support.
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Unique multicenter bonding in boron-rich materials leads to the formation of complicated structures and intriguing properties. ScB6, as a sister compound, possibly possesses high hardness and superconducting critical temperature in this family under ambient pressure. Here, phase transitions, chemical bonding states and electronic properties of ScB6 at high pressure are uncovered using particle swarm optimization (PSO) combined with first-principles calculations. The phase sequence of P21/m â C2/m â Cmcm for ScB6 has been identified under high pressure. Interestingly, the evolution of a boron framework is from a graphene-like layer to a planar B4 ring, B6 and B7 cycle, and non-planar B8 cycle, which interconnect a graphene-like network. These phases of ScB6 are expected to be hard materials due to the excellent mechanical behaviors by the mechanical property calculations. Although the metallic features of the three phases reduce their hardness, the further electron-phonon coupling calculations indicate that the three phases of ScB6 are superconducting phases under high pressures.
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Background: The crucial roles played by lncRNA-AC068228.1 in primary malignant cancer remain poorly understood. This study aimed at examining the clinical significance and evaluating the biological function of AC068228.1 in lung adenocarcinoma (LUAD). Methods: We used data obtained from The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and the Gene Expression Omnibus (GEO) database to examine the expression of AC068228.1 in LUAD patients, and the prognostic and diagnostic value of those levels. Functional experiments were conducted to determine the function of AC068228.1 on LUAD cells. Signaling pathway enrichment analysis of AC068228.1 was conducted using the clusterProfiler and Gene Set Enrichment Analysis (GSEA) software. We analyzed the correlation between AC068228.1 expression and immune infiltration level in LUAD using the single-sample gene set enrichment analysis (ssGSEA) method by the R package GSVA. Results: AC068228.1 expression was significantly elevated in LUAD tissues compared with normal tissues. Higher expression of AC068228.1 was strongly correlated with adverse clinical outcomes and was identified as an independent prognostic marker for LUAD patients. GSEA and infiltration analysis confirmed that AC068228.1 expression was significantly correlated with immune cells infiltrating in LUAD. Knockdown of AC068228.1 inhibited the cell proliferation and cell migration of LUAD. Conclusions: AC068228.1 was upregulated in LUAD and was significantly correlated with adverse clinical outcomes. Meanwhile, it was associated with immune cell infiltration and could be used as a promising diagnostic and prognostic biomarker for LUAD patients.
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A polymer chemical sensor based on lab-on-a-molecule was synthesised by amine-aldehyde polymerisation for selective detection of fluorine ions (F-) and cyanide (CN-). The polymer chemical sensor shows significant absorption and fluorescence changes upon the addition of F- and CN-, which can be observed by the naked eye and optical reactions. The polymer has a higher fluorescence enhancement effect than its monomer and the distance of wavelength of each anion increased which could be applied to better distinguish the two anions.
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Cianetos , Fluoretos , Ânions/química , Cianetos/química , PolímerosRESUMO
Amorphous nanomaterials have aroused extensive interest due to their unique properties. Their performance is highly related with their distinct atomic arrangements, which have no long-range order but possess short- to medium-range order. Herein, an overview of state-of-the-art synthesis methods of amorphous nanomaterials, structural characteristics and their electrochemical properties is presented. Advanced characterization methods for analyzing and proving the local order of amorphous structures, such as X-ray absorption fine structure spectroscopy, atomic electron tomography and nanobeam electron diffraction, are introduced. Various synthesis strategies for amorphous nanomaterials are covered, especially the salt-assisted metal organic decomposition method to prepare ultrathin amorphous nanosheets. Furthermore, the design and structure-activity relationship of amorphous nanomaterials towards electrochemical applications, including electrocatalysts and battery anode/cathode materials, is discussed.
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Designing and modulating the local structure of metal sites is the key to gain the unique selectivity and high activity of single metal site catalysts. Herein, we report strain engineering of curved single atomic iron-nitrogen sites to boost electrocatalytic activity. First, a helical carbon structure with abundant high-curvature surface is realized by carbonization of helical polypyrrole that is templated from self-assembled chiral surfactants. The high-curvature surface introduces compressive strain on the supported Fe-N4 sites. Consequently, the curved Fe-N4 sites with 1.5 % compressed Fe-N bonds exhibit downshifted d-band center than the planar sites. Such a change can weaken the bonding strength between the oxygenated intermediates and metal sites, resulting a much smaller energy barrier for oxygen reduction. Catalytic tests further demonstrate that a kinetic current density of 7.922â mA cm-2 at 0.9â V vs. RHE is obtained in alkaline media for curved Fe-N4 sites, which is 31 times higher than that for planar ones. Our findings shed light on modulating the local three-dimensional structure of single metal sites and boosting the catalytic activity via strain engineering.
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Developing a rapid and low cost approach to access atomically dispersed metal catalysts (ADMCs) supported by carbon is important but still challenging. Here, an electric flash strategy using high voltage for the rapid fabrication of carbon-supported ADMCs within 1 min is reported. Continuous plasma arc results in nitrogen-doped carbon ultrathin nanosheets, while an intermittent spark pulse constructs carbon hollow nanospheres via blasting effect, and both structures are decorated with atomically dispersed cobalt. The latter catalyst shows a half-wave potential of 0.887 V versus RHE (47 mV higher than commercial Pt/C) in an oxygen reduction reaction (ORR) in alkaline media. The authors' work paves the way to rapid synthesis of carbon-supported ADMCs at both low cost and mass production.
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Chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA) are highly prevalent potential risk factors for systemic disease. Previous studies have reported that COPD and OSA are major independent risk factors for cardio or cerebrovascular diseases. The present study aimed to investigate the role of bone marrow mesenchymal stem cells (BMSCs) on vascular injury in a COPDOSA overlap syndrome (OS) rat model. Rats were randomly divided into three groups: Sham, OS model and BMSC. BMSC localization in major organs was detected via confocal laser fluorescence microscopy, and the aortic tissue pathological changes and related genes were measured using hematoxylin & eosin and Masson staining. Genes associated with vascular endothelial cell injury, including endothelin 1, vascular cell adhesion molecule 1 and endothelial nitric oxide synthase, were detected via reverse transcriptionquantitative PCR and western blotting. Apoptosis of vascular endothelial cells was detected using TUNEL and immunofluorescence assays. The endothelial cell marker CD31 in injured vessels was analyzed via immunohistochemistry. BMSCs migrated into the heart, liver, spleen, lung, kidney, brain and aorta in the OS model. The green fluorescence expression of BMSCs demonstrated the highest level in the lung, followed by the aorta. Aortic tissue had a more severe vascular injury and increased apoptosis in the model group compared with the BMSC group. Vascular endothelial cell apoptosis was decreased in the BMSC group compared with the model group. The findings suggested that BMSCs could repair vascular injury by inhibiting endothelial cell damage and apoptosis. These data provide a theoretical basis for the treatment of cardiovascular diseases caused by OS with BMSCs.
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Células da Medula Óssea/metabolismo , Transplante de Células-Tronco Mesenquimais , Doença Pulmonar Obstrutiva Crônica/terapia , Apneia Obstrutiva do Sono/terapia , Lesões do Sistema Vascular/terapia , Aloenxertos , Animais , Células da Medula Óssea/patologia , Modelos Animais de Doenças , Feminino , Células-Tronco Mesenquimais , Doença Pulmonar Obstrutiva Crônica/metabolismo , Doença Pulmonar Obstrutiva Crônica/patologia , Ratos , Ratos Sprague-Dawley , Apneia Obstrutiva do Sono/metabolismo , Apneia Obstrutiva do Sono/patologia , Síndrome , Lesões do Sistema Vascular/metabolismo , Lesões do Sistema Vascular/patologiaRESUMO
Herein, we report a negative pressure pyrolysis to access dense single metal sites (Co, Fe, Ni etc.) with high accessibility dispersed on three-dimensional (3D) graphene frameworks (GFs), during which the differential pressure between inside and outside of metal-organic frameworks (MOFs) promotes the cleavage of the derived carbon layers and gradual expansion of mesopores. In situ transmission electron microscopy and Brunauer-Emmett-Teller tests reveal that the formed 3D GFs possess an enhanced mesoporosity and external surface area, which greatly favor the mass transport and utilization of metal sites. This contributes to an excellent oxygen reduction reaction (ORR) activity (half-wave potential of 0.901â V vs. RHE). Theoretical calculations verify that selective carbon cleavage near Co centers can efficiently lower the overall ORR theoretical overpotential in comparison with intact atomic configuration.
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It is very challenging to design the capacity-approaching labeling schemes for large constellations, such as 32-QAM, in delayed bit-interleaved coded modulation (DBICM). In this paper, we investigate the labeling design for 32-QAM DBICM with various numbers of bits delayed by one time slot. In particular, we aim to obtain the labeling schemes with a high DBICM channel capacity by searching the possible labeling schemes. To reduce the search space of the candidate labeling schemes, we propose the criteria that are necessary for good labeling. Based on the proposed criteria, a three-step search algorithm is proposed to obtain the candidate labeling efficiently. Numerical results demonstrate that the DBICM with our proposed labeling scheme can approach the capacity of 32-QAM within 0.015 dB at an information rate greater than 2.5 bits/symbol.
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Bone marrowderived mesenchymal stem cells (BMSCs) possess potential therapeutic properties for treating patients with chronic obstructive pulmonary disease (COPD), which is characterized by emphysema and obstructive sleep apnea (OSA). However, their effects on overlap syndrome (OS) remain unclear. We investigated the potential therapeutic effects and possible mechanisms of BMSC transplantation in OS rats. To generate the OS model in rats, the animals underwent daily exposure to cigarette smoke and intermittent hypoxia. BMSCs were intravenously injected into rats. At 4 weeks posttransplantation, the severity of emphysema was assessed by lung hematoxylin and eosin (H&E) staining. The levels of oxidative stress and the malondialdehyde (MDA) and superoxide dismutase (SOD) contents in serum and lung were detected. The apoptosis of alveolar septal cells was also detected by TUNEL assay. Finally, we determined the expression of CD31 and VWF in lung tissues by an immunohistochemical (IHC) assay. It was found that BMSCs were able to migrate to the injured lung and aorta tissues. In lung tissues, transplanted BMSCs, some of which had differentiated into endotheliocytes, were found in the alveolar walls. The mean linear intercept (MLI) and pathological scores were higher and the mean alveolar number (MAN) was lower in the OS group than these parameters in the control group. These values were significantly reduced in the OS+BMSC group compared to those in the OS group. The MDA content was decreased and SOD activity was increased in the OS+BMSC group compared to those in the OS group. The apoptotic index of alveolar wall cells in the OS group was higher than that in the OS+BMSC group. The expression levels of CD31 and VWF in alveolar wall cells in the OS group were lower than those in the OS+BMSC group. These results indicate that BMSCs may inhibit the progression of emphysema in the OS model by differentiating into endotheliocytes and suppressing the apoptosis of endotheliocytes and oxidative stress. There is a possibility that the release of growth factors and structural support may be a determinant for the regenerative effects observed following treatment with BMSCs.
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Células da Medula Óssea/metabolismo , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , Doença Pulmonar Obstrutiva Crônica , Apneia Obstrutiva do Sono , Aloenxertos , Animais , Células da Medula Óssea/patologia , Modelos Animais de Doenças , Células-Tronco Mesenquimais/patologia , Doença Pulmonar Obstrutiva Crônica/metabolismo , Doença Pulmonar Obstrutiva Crônica/patologia , Doença Pulmonar Obstrutiva Crônica/terapia , Ratos , Ratos Sprague-Dawley , Apneia Obstrutiva do Sono/metabolismo , Apneia Obstrutiva do Sono/patologia , Apneia Obstrutiva do Sono/terapiaRESUMO
In recent years, drug-abuse problem is growing by leaps and bounds all over the world. The master minds spearheading its proliferation among the youth are difficult to identify, so drug-abuse case has become a hard nut to crack even with the help of best international experts in forensic science and criminology. Because most nations have tightened their controls on traditional drugs, the younger generation is now hooked onto new-type drugs: 1-(3- trifluoromethylphenyl) piperazine (TFMPP), 1-(3-chlorophenyl) piperazine (mCPP) and other new piperazine-drugs, acting as hallucinogens like 'ecstasy', are being consumed by vulnerable masses all over the world. However, only few research studies have focused on developing highly effective detection methods for TFMPP and mCPP in biological fluids; the number of detection methods for these new-type drugs is almost nil in China. Therefore, it is difficult to detect and prevent drug abuse cases related to piperazine drugs in China. There is an urgent need to develop some simple, fast, and reliable methods for detecting piperazine-drugs in vulnerable masses. Thus, the development of novel detection methods with high sensitivity and selectivity is a difficult task for the officials working in the department of forensic science in China. In this work, a new method was developed for the detection of piperazine derivatives: it was performed under the various specific conditions required for conducting chromatography and mass spectrometry analysis. With this novel method, TFMPP and mCPP was successfully detected with high accuracy in various biological samples. By comparing the purification effect of different solid-phase extraction columns for TFMPP and mCPP in biological fluids (urine and blood), we confirmed the validity of the novel method. In addition, this method has good linear relationship and a low detection line when GC/MS was performed for detecting TFMPP, mCPP in the biological fluids (urine and blood). It is a simple, reproducible method that is highly specific in the detection of piperazine-drugs. Thus, it is indeed a reliable method in forensic science.