RESUMO
A concise, racemic total synthesis of three sesquiterpenoid alkaloids (greenwaylactams A-C) exhibiting an unprecedented 8-membered benzolactam is disclosed. Key transformations of this work include the ring expansion through cleavage of an indole via Witkop oxidation, as well as an HFIP mediated cationic cyclisation to build up the pentacyclic carbon skeleton.
RESUMO
The two substituted 1,2,3,4-tetra-hydro-naphthalenes, methyl (R)-3-{(1R,4S)-6-meth-oxy-4,7-dimethyl-5,8-bis-[(triiso-propyl-sil-yl)-oxy]-1,2,3,4-tetra-hydro-naph-th-al-en-1-yl}butano-ate, C36H66O5Si2, (2), and methyl (E)-3-{(1R,4S)-8-hy-droxy-6-meth-oxy-4,7-dimethyl-5-[(triiso-propyl-sil-yl)-oxy]-1,2,3,4-tetra-hydro-naphthalen-1-yl}acrylate, C26H42O5Si, (8), crystallize in the Sohncke space groups P212121 and P21, respectively, with the absolute structure determined on the basis of anomalous dispersion effects. The configurations of the stereo centres in the 1,2,3,4-tetra-hydro-naphthalene moiety of (2) and (8) are the same, and the conformation of the non-aromatic part of the ring system is nearly identical. In the crystal of (2), weak non-classical C-Hâ¯O inter-actions consolidate the packing, whereas in (8), inter-molecular O-Hâ¯O hydrogen-bonding inter-actions of medium-to-weak strength direct the mol-ecules into Z-shaped strands extending parallel to [010].
RESUMO
Dehydrochloromethyltestosterone (DHCMT) is one of the most detected illicit used anabolic-androgenic steroids in professional sports. Therefore, a fast and accurate analysis of this substance is of great importance for a constructive fight against doping abuse. The conventional method for the analysis of this drug, GC-MSMS, is very sensitive and selective but also very time- and resource-consuming. With the presented work, a new approach for simple detection with LC-HRMSMS without any sample preparation is introduced. The method is based on the direct analysis of two newly described phase-II metabolites of the DHCMT long-term metabolite 4-chloro-18-nor-17ß-hydroxymethyl-17α-methyl-5ß-androst-13-en-3α-ol (M3). LC-HRMSMS, GC-MSMS, fractionation and derivatization experiments are combined to identify and characterize for the first time two different glucuronide-acid conjugates of this metabolite in positive human urine samples. In addition, a third glucuronide metabolite was identified, however without isomeric structure determination. The detection of these metabolites is particularly interesting for confirmation analyses, as the method is rapid and requires little sample material.
Assuntos
Anabolizantes , Dopagem Esportivo , Anabolizantes/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Glucuronídeos/urina , Humanos , Detecção do Abuso de Substâncias/métodosRESUMO
The urinary steroid profile established for the monitoring of eventual testosterone or testosterone precursor application by athletes includes concentrations and ratios of various endogenously produced steroidal hormones and metabolites. Due to enzymatic activities in urine specimens, the concentrations of these endogenous steroids and consequently their ratios may alter, leading to potential misinterpretation of analytical results. Microbiological contamination in athletes' urine samples can occur due to urinary tract infections or due to contamination by the non-sterile sample collection conditions. Depending on the duration of transportation of urine samples, the transport and storage conditions may favour microorganisms' growth, and therefore, the enzymatic activity can be accelerated. Degradation effects on endogenous steroids caused by microorganisms have been observed, such as hydrolysis of steroid conjugates, increase of testosterone in the free fraction or modification of the steroid structure by oxidoreductive reactions. The World Anti-Doping Agency (WADA) implemented criteria to check for signs of microbial degradation in a technical document dealing with the detection, analysis and reporting of endogenous androgenic anabolic steroids (TD EAAS) in urine samples. During the endogenous steroid profile confirmation procedures (CPs) of the WADA accredited Seibersdorf Laboratory, significant differences in the concentrations of markers of the steroid profile were observed compared to the initial testing procedures (ITPs). The changes in concentrations of the urinary steroid profile were attributed to the reduction of the 17-keto group to a 17ß-hydroxy group caused by increased enzymatic activity during the hydrolysis step. In order to monitor the 17-keto reduction activity in athletes' urine specimens, possible marker substances containing a 17-keto group were synthesised and added in the internal standards mixture (ISTD) of the ITP. The presence of the reduced 17ß-hydroxy form of the marker substance indicated enzymatic activity leading to 17-keto reduction reactions. The substance 3ß-ethoxy-5α-androstane-17-one was defined to be suitable to indicate 17-keto reduction reactions occurring during hydrolysis carried out at moderate temperatures.
Assuntos
Dopagem Esportivo , Esteroides , Humanos , Esteroides/urina , Congêneres da Testosterona , Testosterona/urina , Atletas , Padrões de Referência , Detecção do Abuso de Substâncias/métodosRESUMO
The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1'N-glucuronide and 17-epistanozolol-2'N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1'N-glucuronide and 17-epistanozolol-1'N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.
Assuntos
Anabolizantes/análise , Dopagem Esportivo/prevenção & controle , Estanozolol/análise , Detecção do Abuso de Substâncias/métodos , Anabolizantes/metabolismo , Anabolizantes/urina , Feminino , Glucuronídeos/análise , Glucuronídeos/urina , Humanos , Limite de Detecção , Masculino , Extração em Fase Sólida/métodos , Estanozolol/metabolismo , Estanozolol/urina , Fatores de TempoRESUMO
We herein report the synthesis of the long-term metabolites "M4" (IUPAC: 4-chloro-17-hydroxymethyl-17-methyl-18-norandrosta-4,13-dien-3-ol) of dehydrochloromethyl-testosterone (DHCMT, Oral Turinabol) and "Oxy M9" (4-hydroxy-17ß-hydroxymethyl-17α-methyl-18-norandrosta-4,13-dien-3-one) of oxymesterone (Oranabol). Both compounds were derived from a common synthetic route starting from dehydroepiandrosterone acetate. Four different stereoisomers were evaluated for metabolite M4. The previously assigned structure could be corrected regarding the C-3 and C-17 stereocenters.
Assuntos
Androstenodióis/metabolismo , Testosterona/análogos & derivados , Humanos , Estrutura Molecular , Análise Espectral/métodos , Estereoisomerismo , Testosterona/química , Testosterona/metabolismoRESUMO
Stanozolol is still the most commonly used illicit anabolic-androgenic steroid (AAS) in professional sports. Therefore, accurate and fast analysis and long detection windows are of great interest in the field of antidoping analysis. In this work, a very simple, fast, and highly sensitive online solid-phase extraction method coupled with liquid chromatography-high-resolution tandem mass spectrometry (HPLC-HRMSMS) for the analysis of stanozolol-N-glucuronides was developed. This fully validated procedure is characterized by only a few manual steps (dilution and addition of internal standard) in the sample preparation. A limit of identification (LOI) of 75 pg/mL, high accuracy (87.1%-102.1%), precision (3.1%-7.8%), and sensitivity was achieved. Furthermore, good linearity (> 0.99) and robustness, as well as no carry-over effects, could be observed. In addition to excellent confirmation analysis performance, this method shows sufficient potential for the identification and characterization of unknown metabolites. Using this method, it was possible to unambiguously confirm the presence of 1'N- and 2'N-stanozolol-glucuronide in human urine for the first time due to the access to reference material.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Glucuronídeos/urina , Estanozolol/urina , Detecção do Abuso de Substâncias/métodos , Anabolizantes/urina , Dopagem Esportivo/prevenção & controle , Feminino , Humanos , Limite de Detecção , Masculino , Reprodutibilidade dos Testes , Extração em Fase Sólida , Espectrometria de Massas em TandemRESUMO
A long-term metabolite of the doping agent oxymetholone (OXM-M2, 17ß-hydroxymethyl-2,17α-methyl-18-norandrost-13-en-3-one) which has been identified by GC-MS/MS was synthesized from commercially available materials. Two efficient synthetic routes to access both C-17 epimers of tentative metabolites were developed. The identity and molecular configuration of the in vivo metabolite: 17ß-hydroxymethyl-2α,17α-methyl-18-norandrost-13-en-3-one was confirmed by single crystal X-ray diffraction.
Assuntos
Oximetolona/síntese química , Oximetolona/metabolismo , Cristalografia por Raios X , Humanos , Modelos Moleculares , Conformação Molecular , Oximetolona/químicaRESUMO
In doping control analysis, the characterization of urinary steroid metabolites is of high interest for a targeted and long-term detection of prohibited anabolic androgenic steroids (AAS). In this work, the structure of a long-term metabolite of dehydrochloromethyltestosterone (DHCMT) was elucidated. Altogether, 8 possible metabolites with a 17α-methyl-17ß-hydroxymethyl - structures were synthesized and compared to a major DHCMT long-term metabolite detected in reference urine excretion samples. The confirmed structure of the metabolite was 4α-chloro-18-nor-17ß-hydroxymethyl-17α-methyl-5α-androst-13-en-3α-ol.
RESUMO
The human urinary long-term metabolite "M3" (4-chloro-17ß-hydroxymethyl-17α-methyl-18-norandrost-13-en-3-ol) of the common doping agent DHCMT has thus far been detected via GC/MS-MS, creating ambiguities concerning its absolute configuration. Its structure was elucidated via the synthesis of all eight possible stereoisomers with 17ß-hydroxymethyl configuration. The highlights of the synthesis consist of a novel first generation approach to 4ß-chloro-5ß compounds as well as a divergent route which allows easy access to the remaining A-ring chlorohydrins.
Assuntos
Testosterona/análogos & derivados , Testosterona/síntese química , EstereoisomerismoRESUMO
The goal of this work was a good-yielding chemical synthesis of a metandienone metabolite which is of interest in doping analysis. 20ßOH-NorMD (IUPAC: 17ß-hydroxymethyl-17α-methyl-18-norandrosta-1,4,13-triene-3-one) has been identified as a long-term urinary metabolite which can be detected and attributed to metandienone up to almost 3weeks after exposure. The chemical synthesis of its epimer 20αOH-NorMD has been described before, as was an enzymatic synthesis of 20ßOH-NorMD, but no chemical synthesis was published.
Assuntos
Metandrostenolona/química , Cromatografia Líquida de Alta Pressão , Desidroepiandrosterona/análogos & derivados , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Trientina/químicaRESUMO
We present the application of ionic liquid-aqueous micellar solutions as reaction media for Diels-Alder reaction and found that reaction rates could be significantly increased compared to the reaction in water.
RESUMO
The detection of metabolites of the anti-estrogenic substance cyclofenil, listed on the World Anti-Doping Agency (WADA) Prohibited List since 2004 is described. Target substances are hydroxylated metabolites, bearing an aliphatic hydroxyl group either in the 2-, 3- or 4-position of the aliphatic ring, in addition to the phenolic functions on the aromatic rings. Structural identification used NMR as well as high-resolution mass spectrometry after nano-electrospray ionisation (ESI). Unambiguous detection of all three synthesised cyclofenil metabolites M1-M3 was done using gas chromatography for separation and electron ionisation mass spectrometry for detection of the per-silylated compounds in comparison with a reference urine deriving from an excretion study within the WADA 2007 Educational Programme.
Assuntos
Ciclofenil , Dopagem Esportivo , Moduladores de Receptor Estrogênico , Drogas Ilícitas/síntese química , Detecção do Abuso de Substâncias/métodos , Cromatografia Gasosa , Ciclofenil/análogos & derivados , Ciclofenil/química , Ciclofenil/urina , Moduladores de Receptor Estrogênico/química , Moduladores de Receptor Estrogênico/urina , Humanos , Hidroxilação , Drogas Ilícitas/urina , Nanotecnologia , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
The first synthesis of 16,16,20,20,20-pentadeuterio-3'-hydroxystanozolol (8) in 26% yield over nine steps is described using moderately priced starting materials and economic amounts of reagents. Compound 8 can be used as an internal standard in screening procedures for anabolic steroids as well as for the quantification of stanozolol metabolites via mass spectrometric techniques, such as LC-MS or gas chromatography-mass spectrometry (GC-MS).
Assuntos
Androsterona/síntese química , Estanozolol/análogos & derivados , Androsterona/química , Cromatografia Líquida/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Espectrometria de Massas/métodos , Conformação Molecular , Padrões de Referência , Estanozolol/síntese química , Estanozolol/normasRESUMO
A facile six-step synthesis of 2,2,3,4,4-d5-androsterone-beta-D-glucuronide (1) starting from epiandrosterone (2) in 63% yield is described and compared with several alternative synthetic pathways. Compound 1 can be used as an internal standard in screening procedures for anabolic steroids to monitor the hydrolysis step of the steroid glucuronides prior to gas chromatography-mass spectrometry (GC-MS) analysis. Thus, a time consuming solid-phase extraction step to remove possible hydrolysis inhibitors can be omitted.