Iron-enhanced microscale laboratory aerated filters in the treatment of artificial mariculture wastewater: A study on nitrogen removal performance and the impact on microbial community structure.

This study investigates the nitrogen removal efficacy and microbial community dynamics in seawater aquaculture effluent treatment using three different substrate combinations of microscale laboratory aerated filters (MFs) - MF1 (LECA), MF2 (LECA/Fe-C), and MF3 (LECA/Pyrite). The findings indicated that the COD removal exceeded 95% across all MFs, with higher removal efficiencies in MF2 and MF3. In terms of nitrogen removal performance, MF2 exhibited the highest average nitrogen removal of 93.17%, achieving a 12.35% and 3.56% increase compared to MF1 (80.82%) and MF3 (89.61%), respectively. High-throughput sequencing analysis revealed that the Fe-C substrate significantly enhanced the diversity of the microbial community. Notably, in MF2, the salinophilic denitrifying bacterium Halomonas was significantly enriched, accounting for 42.6% of the total microbial community, which was beneficial for nitrogen removal. Moreover, an in-depth analysis of nitrogen metabolic pathways and microbial enzymes indicated that MF2 and MF3 possessed a high abundance of nitrification and denitrification enzymes, related to the high removal rates of NH4+-N and NO3--N. Therefore, the combination of LECA with iron-based materials significantly enhances the nitrogen removal efficiency from mariculture wastewater.

Removal of sulfur and nitrogen pollutants in a sediment microbial fuel cell coupled with Vallisneria natans: Efficiency, microbial community structure, and functional genes.

The rapid development of the economy has led to an increase in the sulfur and nitrogen load in surface water, which has the potential to cause river eutrophication and the emission of malodorous gases. A lab-scale sediment microbial fuel cell coupled with Vallisneria natans (P-SMFC) was designed for surface water remediation. The enhancement of pollutant removal performance of P-SMFC was evaluated in contrast to the SMFC system without plants (SMFC), the open-circuit control system with plants (C-P), and the open-circuit control system without plants (C-S), while illustrating the mechanisms of the sulfur and nitrogen transformation process. The results demonstrated that the effluent and sediment of P-SMFC had lower concentrations of sulfide compared to other systems. Furthermore, P-SMFC exhibited higher removal efficiency for COD (73.1 ± 8.7%), NH4+-N (80.5 ± 19.8%), and NO3--N (88.5 ± 11.8%) compared to other systems. The closed-circuit conditions and growth of Vallisneria natans create a favorable ecological niche for functional microorganisms involved in power generation, sulfur oxidation, and nitrogen transformation. Additionally, metagenomic analysis revealed that multifunctional bacteria possessing both denitrification and sulfur oxidation genes, such as Thiobacillus, Dechloromonas, and Bacillus, may play simultaneous roles in metabolizing sulfur and nitrogen, thus serving as integral factors in maintaining the performance of P-SMFC. In summary, these findings provide a theoretical reference for the concurrent enhancement of sulfur and nitrogen pollutants removal in P-SMFC and will facilitate its practical application in the remediation of contaminated surface water.

Endothelial dysfunction of syphilis: Pathogenesis.

Treponema pallidum is the causative factor of syphilis, a sexually transmitted disease (STD) characterized by perivascular infiltration of inflammatory cells, vascular leakage, swelling and proliferation of endothelial cells (ECs). The endothelium lining blood and lymphatic vessels is a key barrier separating body fluids from host tissues and is a major target of T. pallidum. In this review, we focus on how T. pallidum establish intimate interactions with ECs, triggering endothelial dysfunction such as endothelial inflammation, abnormal repairment and damage of ECs. In addition, we summarize that migration and invasion of T. pallidum across vascular ECs may occur through two pathways. These two mechanisms of transendothelial migration are paracellular and cholesterol-dependent, respectively. Herein, clarifying the relationship between T. pallidum and endothelial dysfunction is of great significance to provide novel strategies for diagnosis and prevention of syphilis, and has a great potential prospect of clinical application.

Combatting ageing in dermal papilla cells and promoting hair follicle regeneration using exosomes from human hair follicle dermal sheath cup cells.

Dermal papilla cells (DPCs) undergo premature ageing in androgenetic alopecia and senescent alopecia. As critical components of hair follicle reconstruction, DPCs are also prone to senescence in vitro, resulting in a diminished hair follicle inductivity capacity. Dermal sheath cup cells (DSCCs), a specific subset of hair follicle mesenchymal stem cells, intimately linked to the function of DPCs. The primary objective of this research is to investigate the anti-ageing effect of exosomes derived from DSCCs (ExoDSCCs ) on DPCs. Exosomes were utilized to treat H2 O2 -induced DPCs or long-generation DPCs(P10). Our findings demonstrate that ExoDSCCs(P3) promote the proliferation, viability and migration of senescent DPCs while inhibiting cell apoptosis. The expression of senescence marker SA-ß-Gal were significantly downregulated in senescent DPCs. When treated with ExoDSCCs(P3) , expression of inducibility related markers alkaline phosphatase and Versican were significantly upregulated. Additionally, ExoDSCCs(P3) activated the Wnt/ß-catenin signalling in vitro. In patch assay, ExoDSCCs(P3) significantly promoted hair follicle reconstruction in senescent DPCs. In summary, our work highlights that ExoDSCCs(P3) may restore the biological functions and improve the hair follicle induction ability of senescent DPCs. Therefore, ExoDSCCs(P3) may represent a new strategy for intervening in the ageing process of DPCs, contributing to the prevention of senile alopecia.

Resurgence of syphilis: focusing on emerging clinical strategies and preclinical models.

Syphilis, a sexually transmitted disease (STD) caused by Treponema pallidum (T. pallidum), has had a worldwide resurgence in recent years and remains a public health threat. As such, there has been a great deal of research into clinical strategies for the disease, including diagnostic biomarkers and possible strategies for treatment and prevention. Although serological testing remains the predominant laboratory diagnostic method for syphilis, it is worth noting that investigations pertaining to the DNA of T. pallidum, non-coding RNAs (ncRNAs), chemokines, and metabolites in peripheral blood, cerebrospinal fluid, and other bodily fluids have the potential to offer novel perspectives on the diagnosis of syphilis. In addition, the global spread of antibiotic resistance, such as macrolides and tetracyclines, has posed significant challenges for the treatment of syphilis. Fortunately, there is still no evidence of penicillin resistance. Hence, penicillin is the recommended course of treatment for syphilis, whereas doxycycline, tetracycline, ceftriaxone, and amoxicillin are viable alternative options. In recent years, efforts to discover a vaccine for syphilis have been reignited with better knowledge of the repertoire of T. pallidum outer membrane proteins (OMPs), which are the most probable syphilis vaccine candidates. However, research on therapeutic interventions and vaccine development for human subjects is limited due to practical and ethical considerations. Thus, the preclinical model is ideal for conducting research, and it plays an important role in clinical transformation. Different preclinical models have recently emerged, such as in vitro culture and mouse models, which will lay a solid foundation for clinical treatment and prevention of syphilis. This review aims to provide a comprehensive summary of the most recent syphilis tactics, including detection, drug resistance treatments, vaccine development, and preclinical models in clinical practice.

Research progress on the mechanism of Treponema pallidum breaking through placental barrier.

Congenital syphilis, a significant cause of fetal mortality worldwide, is a congenital infectious disease instigated by the vertical transmission of Treponema pallidum during pregnancy. Clinical manifestations include preterm delivery, stillbirth, neonatal skin lesions, skeletal abnormalities, and central nervous system aberrations. The ongoing increase in the incidence of congenital syphilis, coupled with complexities in diagnosis, necessitates a detailed understanding of its pathogenesis for the development of improved diagnostic approaches, and to interrupt the route of vertical transmission. Drawing from the broader body of research associated with vertical transmission pathogens, we aim to clarify the potential mechanisms by which Treponema pallidum breaches the placental barrier to infect the fetus.

The novel angiogenesis regulator circFAM169A promotes the metastasis of colorectal cancer through the angiopoietin-2 signaling axis.

BACKGROUND: Angiogenesis plays an important role in the metastasis of cancers. However, the mechanisms whereby circular RNAs (circRNAs) regulate angiogenesis and affect cancer metastasis are still unclear. METHODS: We used gene set variation and Spearman's correlation analyses to identify novel angiogenesis-related circRNAs, including circFAM169A. The Kyoto Encyclopedia of Genes and Genomes and Gene Ontology were used to assess the potential biological function of circFAM169A. A quantitative reverse transcription-PCR (qRT-PCR) analysis of 20 pairs of colorectal cancer (CRC) samples was performed to detect the expression level of circFAM169A. Transwell assays, tube formation assays, and nude mouse metastatic tumor models were used to study the function of circFAM169A in CRC. qRT-PCR, dual-luciferase reporter gene assay, RNA antisense purification assay, and Western blot were performed to analyze the competing endogenous RNA mechanism of circFAM169A in promoting CRC angiogenesis. RESULTS: circFAM169A was highly correlated with the hallmark of angiogenesis in CRC patients. It was up-regulated in liver metastasized CRC patients. circFAM169A overexpression promoted the angiogenesis, migration, and invasion of CRC cells while its down-regulation had the opposite effects. In vivo mouse models further highlighted the pro-metastatic role of circFAM169A in CRC. More importantly, we discovered that circFAM169A enhances the expression of angiopoietin-2 by binding to miR-518a-5p.

Effects of chemical oxygen demand/nitrogen on electrochemical performances and denitrification efficiency in single-chamber microbial fuel cells: Insights from electron transfer and bacterial communities.

The electrochemical performances and denitrification efficiency of microbial fuel cells (MFCs) are often limited by chemical oxygen demand/nitrogen (COD/N) of wastewater. To overcome this limitation, single-chamber air cathode MFCs with varying COD/N (16/1, 8/1, and 4/1) were established to investigate their electrochemical performances, denitrification efficiency, and bacterial communities. The optimal COD/N for maximizing electricity generation and denitrification efficiency was 8/1, as supported by the greatest corrected coulomb efficiency (13.6%) and electron transfer rate (2.36 C/h for electricity generation, 39.77 C/h for denitrification). As COD/N decreased, the electrochemically active genus Geobacter was replaced by the denitrifying genera Un._f_Burkholderiaceae, Dechlorosoma, and Petrimonas. These results indicated that the efficiency of electricity generation and denitrification was not solely determined by the abundance of electrochemically active and denitrifying bacteria. The presence of a faster electron transfer pathway, possibly direct interspecies electron transfer, enhanced simultaneous electricity generation and denitrification in MFCs with COD/N of 8/1.

Simultaneous nitrate and sulfate biotransformation driven by different substrates: comparison of carbon sources and metabolic pathways at different C/N ratios.

Nitrate (NO3-) and sulfate (SO42-) often coexist in organic wastewater. The effects of different substrates on NO3- and SO42- biotransformation pathways at various C/N ratios were investigated in this study. This study used an activated sludge process for simultaneous desulfurization and denitrification in an integrated sequencing batch bioreactor. The results revealed that the most complete removals of NO3- and SO42- were achieved at a C/N ratio of 5 in integrated simultaneous desulfurization and denitrification (ISDD). Reactor Rb (sodium succinate) displayed a higher SO42- removal efficiency (93.79%) with lower chemical oxygen demand (COD) consumption (85.72%) than reactor Ra (sodium acetate) on account of almost 100% removal of NO3- in both Ra and Rb. Ra produced more S2- (5.96 mg L-1) and H2S (25 mg L-1) than Rb, which regulated the biotransformation of NO3- from denitrification to dissimilatory nitrate reduction to ammonium (DNRA), whereas almost no H2S accumulated in Rb which can avoid secondary pollution. Sodium acetate-supported systems were found to favor the growth of DNRA bacteria (Desulfovibrio); although denitrifying bacteria (DNB) and sulfate-reducing bacteria (SRB) were found to co-exist in both systems, Rb has a greater keystone taxa diversity. Furthermore, the potential carbon metabolic pathways of the two carbon sources have been predicted. Both succinate and acetate could be generated in reactor Rb through the citrate cycle and the acetyl-CoA pathway. The high prevalence of four-carbon metabolism in Ra suggests that the carbon metabolism of sodium acetate is significantly improved at a C/N ratio of 5. This study has clarified the biotransformation mechanisms of NO3- and SO42- in the presence of different substrates and the potential carbon metabolism pathway, which is expected to provide new ideas for the simultaneous removal of NO3- and SO42- from different media.

A diagnostic method for cardiomyopathy based on multimodal data.

OBJECTIVES: Currently, a multitude of machine learning techniques are available for the diagnosis of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) by utilizing electrocardiography (ECG) data. However, these methods rely on digital versions of ECG data, while in practice, numerous ECG data still exist in paper form. As a result, the accuracy of the existing machine learning diagnostic models is suboptimal in practical scenarios. In order to enhance the accuracy of machine learning models for diagnosing cardiomyopathy, we propose a multimodal machine learning model capable of diagnosing both HCM and DCM. METHODS: Our study employed an artificial neural network (ANN) for feature extraction from both the echocardiogram report form and biochemical examination data. Furthermore, a convolutional neural network (CNN) was utilized for feature extraction from the electrocardiogram (ECG). The resulting extracted features were subsequently integrated and inputted into a multilayer perceptron (MLP) for diagnostic classification. RESULTS: Our multimodal fusion model achieved a precision of 89.87%, recall of 91.20%, F1 score of 89.13%, and precision of 89.72%. CONCLUSIONS: Compared to existing machine learning models, our proposed multimodal fusion model has achieved superior results in various performance metrics. We believe that our method is effective.

Melatonin influences the biological characteristics of keloid fibroblasts through the Erk and Smad signalling pathways.

Background: Keloids are abnormal fibrous hyperplasias that are difficult to treat. Melatonin can be used to inhibit the development of certain fibrotic diseases but has never been used to treat keloids. We aimed to discover the effects and mechanisms of melatonin in keloid fibroblasts (KFs). Methods: Flow cytometry, CCK-8 assays, western blotting, wound-healing assays, transwell assays, collagen gel contraction assays and immunofluorescence assays were applied to demonstrate the effects and mechanisms of melatonin in fibroblasts derived from normal skin, hypertrophic scars and keloids. The therapeutic potential of the combination of melatonin and 5-fluorouracil (5-FU) was investigated in KFs. Results: Melatonin significantly promoted cell apoptosis and inhibited cell proliferation, migration and invasion, contractile capability and collagen production in KFs. Further mechanistic studies demonstrated that melatonin could inhibit the cAMP/PKA/Erk and Smad pathways through the membrane receptor MT2 to alter the biological characteristics of KFs. Moreover, the combination of melatonin and 5-FU remarkably promoted cell apoptosis and inhibited cell migration and invasion, contractile capability and collagen production in KFs. Furthermore, 5-FU suppressed the phosphorylation of Akt, mTOR, Smad3 and Erk, and melatonin in combination with 5-FU markedly suppressed the activation of the Akt, Erk and Smad pathways. Conclusions: Collectively, melatonin may inhibit the Erk and Smad pathways through the membrane receptor MT2 to alter the cell functions of KFs, while combination with 5-FU could exert even more inhibitory effects in KFs through simultaneous suppression of multiple signalling pathways.

Effects of various COD/NO ratios on NOx removal performance and microbial communities in a BTF-ABR integrated system.

In this study, we investigated the removal performance of NOx and stability of the biotrickling filter-anaerobic baffled reactor (BTF-ABR) integrated system at various chemical oxygen demand (COD)/NO ratios (12.18, 6.71, and 4.63 in stages 1, 2, and 3, respectively) under 3.5% O2 and 50 ± 0.5 °C conditions for the first time. The results showed that the maximum elimination capacity of NOx was 4.46, 8.16, and 11.58 g/(m3·h) in stages 1, 2, and 3, respectively. The minimum operating cost in terms of glucose was 4.79 g of glucose/g of NO. However, a COD/NO ratio of 12.18 resulted in a wastage of carbon sources, while a COD/NO ratio of 4.63 led to about 20 mg/m3 N2O emission at the end of the study. Highly bacteria diversity and positive co-occurrence networks at the COD/NO ratio of 6.71 were the main reasons for no intermediate accumulation or N2O emission. Analysis of real-time polymerase chain reaction (PCR) indicated that nirS and norB were more sensitive to the changes in the COD/NO ratios than other denitrifying genes, and the denitrifiers with nirS filled more ecological niches as the NOx increased. Furthermore, although the decrease in COD/NO ratio significantly impacted the microbial community structure, the NOx RE was stabilized at over 90% because the micro-aerobic environment produced by ABR combined highly diverse microbes and functions in BTF, as well as the coordinated expression of denitrifying genes. Achieving efficient, stable, and low-cost denitrification is feasible in this BTF-ABR integrated system.

Complexing agents-free bioelectrochemical trickling systems for highly-efficient mesothermal NO removal: The role of extracellular polymer substances.

The biological treatments are promising for nitric oxide (NO) reduction, however, the biotechnology has long suffered from high demands of NO-complexing agents (i.e., Fe(II)EDTA), leading to extra operation costs. In this study, novel complexing agents-free bioelectrochemical systems have been developed for direct NO reduction. The electricity-driven bioelectrochemical trickling system (ED-BTS, a denitrifying biocathode driven by the external electricity and an acetate-consuming bioanode) achieved approximately 68% NO removal without any NO-complexing agents, superior to the bioanode-driven BTS and open-circuit BTS. The extracellular polymeric substances from the biofilms of ED-BTS contained more polysaccharides, humic substrates, and hydrophobic tryptophan that were beneficial for NO reduction. Additionally, the external electricity altered the microbial community toward more denitrifying bacteria and a higher abundance of NO reduction genes (nosZ and cnorB). This study provides a comprehensive understanding of microbial behaviors on the adsorption and reduction of NO and proposes a promising strategy for mesothermal NO biotreatment.

Visible-light-driven simultaneous decontamination of multi-antibiotics by facile synthesized BiOCl loaded food wastes biochar.

Environmental dissemination caused by widespread use of antibiotics has been regarded as a possible hazard to aquatic ecosystem and human health. The increasing misgivings make it imperative to develop a novel catalyst with remarkable visible-light-driven activity to remove antibiotics, especially for their simultaneous decontamination. Herein, C/BiOCl composites were successfully prepared by decorating BiOCl nanosheets on food wastes biochar (C) by a simple hydrolysis strategy. Not only the binary system of tetracycline antibiotics, but also the ternary mixture could be simultaneously photodegraded over 25% C/BiOCl within 15 min irradiation. The improved photocatalytic activities could be ascribed to the introduction of biochar, endowing increased surface area, enhanced separation of photo-generated charge carriers, and better light absorption. The as-prepared 25% C/BiOCl also demonstrated satisfactory stability and positive removal effect in actual water samples. The present work provides new insights into the development of biochar-based photocatalysts for simultaneous degradation of multiple antibiotics.

Negative-Pressure Wound Therapy Improves Take Rate of Skin Graft in Irregular, High-Mobility Areas: A Randomized Controlled Trial.

BACKGROUND: Tie-over bolster dressing to secure a skin graft is associated with low graft take rates in irregular, high-mobility areas and suboptimal recipient wound beds. Negative-pressure wound therapy has become a well-established method to secure the graft, with a graft take rate of this method reported to be 96.7 percent. However, comparative efficacies between the two methods on irregular, high-mobility areas are yet to be determined. METHODS: Patients eligible for skin graft were randomly assigned to receive either negative-pressure wound therapy or tie-over bolster dressing between December of 2014 and December of 2015. The primary outcome was determined by the take rate of skin grafts between postoperative days 5 and 7. The secondary outcomes were dressing time and postoperative complications, including hematoma, seroma, infection, displacement, and necrosis. RESULTS: A total of 86 patients were assigned to receive either negative-pressure wound therapy ( n = 43) or tie-over bolster dressing ( n = 43) for skin graft treatment. Negative-pressure wound therapy significantly improved the take rate of grafts as compared with tie-over bolster dressing (97.2 versus 90.2 percent; p = 0.005). The improvements came from the grafts in irregular, high-mobility areas in the respective groups (97.6 versus 81.7 percent; p < 0.001). Negative-pressure wound therapy reduced skin graft displacement as a postoperative complication as compared with tie-over bolster dressing (one versus nine patients; p = 0.007). Dressing time using negative-pressure wound therapy was significantly shorter compared with tie-over bolster dressing (15.2 ± 4.2 versus 27.4 ± 4.3 minutes; p = 0.001). CONCLUSION: Negative-pressure wound therapy can improve the take rate of skin grafts in irregular, high-mobility areas and shorten the dressing time. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, I.

Kitchen-waste-derived biochar modified nanocomposites with improved photocatalytic performances for degrading organic contaminants.

Kitchen-waste-derived biochar (KBC) was produced by thermal treatment at 400 °C, and a series of KBC/BiOX (X = Br, Cl) photocatalysts were developed using ultrasonication and solvothermal treatment. The as-prepared photocatalysts were characterized by several tests and investigated by photocatalytic reactions towards methyl orange (MO) and tetracycline (TC). The best photocatalysts, 0.15KBC/BiOBr and 0.15KBC/BiOCl separately achieved complete MO photodegradation in 20 min and 35 min. Further study confirmed that 0.15KBC/BiOBr and 0.15KBC/BiOCl possessed excellent photocatalytic efficiency that was 17.9 and 14.8 times higher than BiOBr and BiOCl, respectively. In addition, 0.15KBC/BiOX showed higher activity removal of TC than pure BiOX in 60 min. Notably, 0.15KBC/BiOX maintained a reproducible high photocatalytic efficiency after five recycles. Estimated band gap energy for 0.15KBC/BiOBr (2.40 eV) and 0.15KBC/BiOCl (3.00 eV) was considerably lower than that of BiOBr (2.73 eV) and BiOCl (3.30 eV), indicating a delocalized state was created when forming electronic pathways on the interface. Besides, visible-light harvesting of photocatalysts got promoted by the modification of KBC. Active species trapping experiments and electron paramagnetic resonance (EPR) tests illustrated that photogenerated holes were the principal active species, while ∙OH was involved in the reaction. The successful synthesis of 0.15KBC/BiOX catalyst provided a new approach on simultaneously degrading organic contaminants in water and disposing of excessive kitchen waste.

Effects of Vallisneria natans on H2S and S2- releases in black-odorous waterbody under additional nitrate: Comprehensive performance and microbial community structure.

Releases of hydrogen sulphide (H2S) and sulphur ions (S2-) through sulphate reduction in black-odorous waterbody is a great environmental health concern. Aquatic planting for blackening and odour controls has received great attention in research and practice. Nitrate concentration in black-odorous waterbody can vary significantly but little is known about the responses of aquatic plants on H2S and S2- releases under different nitrate levels. This controlled laboratory study explored the changes of H2S and S2- releases in simulated black-odorous waterbody planted with Vallisneria natans and artificial plants (control). V. natans growth was stimulated by additional nitrate (6.6 mg/L NO3--N), resulting in an increase of dissolved oxygen (DO) and pH in overlying water and an 11.0% decrease in removal efficiency of chemical oxygen demand (COD). At relatively low nitrate level (i.e., 2.0 mg/L NO3--N in the absence of additional nitrate), V. natans after the 48th day inhibited H2S and S2- releases by 81.5% and 66.8%, respectively, and their inhibition efficiencies were improved to 95.7% and 98.8% by the presence of additional nitrate. Additional nitrate reduced the relative abundance of sulphate-reducing bacteria (SRB) in the sediments while increased the relative abundance of sulphur-oxidizing bacteria (SOB) and nitrate-reducing sulphur-oxidizing bacteria (NR-SOB) in the leaf biofilms of V. natans and artificial plants. Genus compositions in leaf biofilms showed host specificity. Pearson correlation analysis showed that DO, pH, and nitrate concentration had a positive correlation with the relative abundance of SOB (Aeromonas) and NR-SOB (Hydrogenophaga), while were negatively correlated with the relative abundance of SRB (MSBL7). These results indicated that V. natans under additional nitrate altered microbial community to be unfavourable for H2S and S2- releases. This study clarified the inhibition of H2S and S2- releases by aquatic planting under additional nitrate and provided theoretical basis for improving black-odorous waterbody restoration technology.

New insights into the efficient charge transfer by construction of adjustable dominant facet of BiOI/CdS heterojunction for antibiotics degradation and chromium Cr(VI) reduction under visible-light irradiation.

The narrow light-response range and high electron/hole recombination rate greatly restrict the widespread use of photocatalytic technology. The integration of exposing dominant facet of semiconductor and Z-scheme heterostructures designing is expected to break those barriers. Herein，In this work, hydrothermal and ultrasonic stirring methods were used to selectively exposed the (001) and (110) facet of BiOI to construct the BiOI/CdS heterostructures. The obtained BiOI(001)/CdS material shown the maximum degradation for tetracycline-based antibiotics (Oxytetracycline, Tetracycline and Doxycycline), and excellent reduction of hexavalent chromium. Combining the electron spin resonance and scavenger experiments, the superior photocatalytic capacity was attributed to the generation of superoxide and hydroxyl radicals. DFT calculation results shown BiOI(001)/CdS performed high binding energy and adsorption energy for hexavalent chromium, and the different work function between BiOI(001) and CdS confirmed the building of internal electric field, thereby increased the charge separation. Finally, the Gaussian 09 and HPLC-MS program investigated the attack sites of free radicals and degradation pathways in the degradation of antibiotics. This study not only provides a potential photocatalyst, also gives an in-depth understanding of the photocatalytic properties of heterojunctions constructed by different exposed crystal facets.

Improving photocatalytic activity under visible light over a novel food wastes biochar-based BiOBr nanocomposite.

Biochar (C) applied in synthesizing photocatalysts to eliminate water pollution has been intensively investigated. Herein we report the first use of biochar pyrolyzed from food wastes at 400 °C (400C) and 700 °C to construct C/BiOBr composites via a facile hydrolysis approach. Photocatalytic performances could be significantly improved by choosing the appropriate carbonization temperature and adjusting the content of C in C/BiOBr composites. The prepared 1%400C/BiOBr exhibited the best photodegradation capacity towards methylene orange (20 mg/L) and tetracycline (50 mg/L). A series of characterization results illustrated that smooth structure and surface properties (oxygen functional groups and persistent free radicals) of 400C played an important role in enhancing the photocatalytic activities. Mechanism exploration suggested that h+ and ˙O2- were the main active species thus contributing to photodegradation. This study provided a new insight into utilization of biochar derived from food wastes in photocatalysis and environmental remediation.

Prediction of Outcomes of Ultrasound-Guided Saline Enema in the Treatment of Pediatric Intussusception: A Retrospective Case-Control Study.

OBJECTIVES: Outcomes of ultrasound-guided saline enema include successful treatment, unsuccessful treatment, or recurrence. This study aimed to investigate the value of ultrasonic parameters of the ileocecal region during hydrostatic reduction to predict enema outcomes. METHODS: Ultrasound images of patients diagnosed with ileocolic intussusception and treated with ultrasound-guided saline enema at two different institutions between January 2019 and April 2021 were retrospectively analyzed to assess ileocecal-valve diameter (ICVD), intussusceptum thickness (IT), and the ratio of IT to ICVD (I/I). Logistic regression analysis was used to explore correlations between ICVD, IT, I/I, and patient characteristics (sex, age, symptom duration, and enema outcome). RESULTS: Of 291 patients with ileocolic intussusception (207 boys; mean ICVD, 8.6 [SD: 0.1] mm; mean IT, 26 [SD: 0.2] mm; mean I/I, 3.0 [SD: 0.01]), 268 had first successful reduction; 23, first failed reduction; 7, final failed reduction; and 41, early recurrence. Significant risk factors for failed reduction included symptom duration >24 hours (odds ratio [OR] = 10, P = .012), ICVD ≤ 8.5 mm (OR = 8, P = .01), and I/I > 3.25 (OR = 16, P < .001). Significant risk factors for early recurrence post-enema included age >1 year (OR = 10, P = .028), ICVD > 8.5 mm (OR = 4, P = .003), and I/I ≤ 2.95 (OR = 6, P < .001). CONCLUSIONS: ICVD and IT measured during ultrasound-guided hydrostatic reduction can predict enema outcomes. The mismatch between IT and ICVD is the primary cause of poor outcomes.