Oxidative response to accumulation of trace metals in tissue of two bivalves, the Asian green mussel Perna viridis and the blood cockle Tegillarca granosa, living in Pattani Bay, Thailand.

OBJECTIVE: Using bivalves to indicate aquatic pollutants was favorable for discerning the negative effects of high levels of metal accumulation in tissue. We investigated the correlation between trace metal accumulation and the tissue oxidative response of two bivalves. METHODS: The Asian green mussel Perna viridis and the blood cockle Tegillarca granosa were sampled along with seawater and sediments from three locations around Pattani Bay, Thailand. Accumulation of nine trace metals (cadmium, cobalt, copper, chromium, nickel, manganese, iron, zinc, and lead) in seawater, sediments, and tissue and the oxidative tissue response were evaluated. Metal bioaccumulation factor, biota-sediment accumulation factor, and histopathology were also indicated. RESULT: The present study found that P. viridis and T. granosa were macroconcentrators and bioaccumulative of cadmium, and their tissue accumulation of cadmium was strongly related to lipid peroxidation activation. Perna viridis exhibited a higher oxidative response than T. granosa, as indicated by malondialdehyde, catalase, and reduced glutathione levels. CONCLUSION: The present study indicated that P. viridis and T. granosa were macroconcentrators and bioaccumulative of cadmium, and their tissue accumulation of cadmium was strongly related to lipid peroxidation activation. Research has shown discernible negative effects of a high level of metal accumulation in tissue, and deformed and damaged tissues were present in the gills, digestive glands, intestines, and feet of P. viridis and T. granosa.

First report on clinical aspects, blood profiles, bacterial isolation, antimicrobial susceptibility, and histopathology in canine pyometra in Thailand.

Background and Aim: Canine pyometra, either the closed (closed pyometra [CP]) or open (open pyometra [OP]) cervix type, is a frequent uterine disease in intact old age bitches. Therefore, early diagnosis and appropriate medical and surgical treatments are crucial to avoid the life-threatening condition in these bitches. This study aimed to investigate clinical alterations, blood parameters, causative bacteria, antimicrobial susceptibility, and uterine histopathology obtained during aseptic surgical treatment on bitches with pyometra. Materials and Methods: Sixty bitches of various breeds and ages with presumptive pyometra diagnoses were included in the study. The diagnoses were based on history, clinical examination, blood parameters, radiography, and ultrasonography. All pyometra bitches were ovariohysterectomized as an emergency surgical treatment. In addition, uterine content and tissues were submitted for bacterial isolation, antimicrobial susceptibility, and uterine histopathological analysis. Results: Except for abdominal CP distention, no specific clinical signs were linked to the pyometra type. The mean values of total white blood cell count (WBC) and plasma protein were predominantly raised in pyometra bitches regarding hematological parameters. Leukocytosis was found in both types; however, the WBC in CP was markedly higher than in OP. The mean value of blood urea nitrogen increased in the CP group. Klebsiella pneumoniae and Escherichia coli were the most frequent causative bacteria isolated in CP and OP, respectively. All isolated bacteria were 100% susceptible to imipenem, meropenem, and carbapenem. Marbofloxacin was the second most effective drug against isolated bacteria from both groups. Uncomplicated cystic endometrial hyperplasia (CEH) was not presented in the CP group. CEH and chronic endometritis (type IV), the most severe uterine histopathological changes, were discovered in the CP and OP. Conclusion: The CP and OP groups presented leukocytosis, increased plasma protein, and CEH and chronic endometritis. Depression, abdominal distention, and enlarged uterine size were the major characteristics of the CP group. Furthermore, abdominal distension is presented in other abnormalities in clinical practices, providing a differential diagnosis. Drugs in the carbapenem group were the most effective against isolated bacteria; however, they are not routinely used due to bacterial resistance concerns. Thus, marbofloxacin was recommended as an alternative medical treatment because it is convenient to manage by both oral and injection routes.

Human Health Risk Assessment of Heavy Metal Concentration in Seafood Collected from Pattani Bay, Thailand.

A significant impact of marine pollution is the contamination of seafood which has raised concerns due to its potential human health risks. This current study investigated seasonal bioaccumulation of 9 heavy metals (Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in 14 commercially important seafood species, including 4 fish, 5 molluscs, and 5 crustacean species. Samples were collected from Pattani Bay, Pattani province, Thailand, during the dry (July 2020) and wet (February 2021) seasons. The edible samples were analyzed for heavy metal concentrations using a flame atomic absorption spectrophotometer. The bioaccumulation trend of heavy metals decreased in the sequence of molluscs > crustaceans > fish. The possible human health risks associated with heavy metal-contaminated seafood consumption were assessed. The parameters investigated for non-carcinogenic and carcinogenic were target hazard quotient (THQ), total hazard index (HI), and target cancer risk (TR). The average ranges of THQs (7.79 × 10−8−8.97 × 10−3), HIs (4.30 × 10−5−1.55 × 10−2), and TRs (2.70 × 10−9−1.34 × 10−5) were observed in the studied seafood species. The results revealed no non-carcinogenic and carcinogenic health risks from consuming these 14 kinds of seafood.

Evaluation of Aflatoxin Concentrations and Occurrence of Potentially Toxigenic Fungi in Imported Chia Seeds Consumed in Thailand.

ABSTRACT: This study was conducted to investigate possible contamination by aflatoxins (AFs) and aflatoxigenic fungi in imported chia seeds consumed in Thailand. A survey was performed on 100 samples of imported chia seeds collected from supermarkets and health food stores in Bangkok from May 2017 to February 2018. Ten mold species belonging to Aspergillus and Penicillium were isolated, and Aspergillus flavus was the most prevalent aflatoxigenic fungi. Chia seed samples were cleaned with an immunoaffinity column and analyzed for AFs by high-performance liquid chromatography with fluorescence detection using precolumn derivatization. AFs were detected in 40% of total samples at concentrations of 0.4 to 10.99 ng/g. Among the positive samples, three were contaminated with total AFs at concentrations higher than the European Union regulatory limit (4 ng/g). The most commonly found AF found in chia seeds was AFB1.

Pharmacokinetics of tolfenamic acid in Hawksbill turtles (Eretmochelys imbricata) after single intravenous and intramuscular administration.

To the best of our knowledge, limited pharmacokinetic information to establish suitable therapeutic plans is available for Hawksbill turtles. Therefore, the present study aimed to assess the pharmacokinetic features of tolfenamic acid (TA) in Hawksbill turtles, Eretmochelys imbricata, after single intravenous (i.v.) and intramuscular (i.m.) administration at dosage 4 mg/kg body weight (b.w.). The study (parallel design) used 10 Hawksbill turtles randomly divided into equal groups. Blood samples were collected at assigned times up to 144 hr. The concentrations of TA in plasma were quantified by a validated liquid chromatography tandem mass spectrometry (LC-ESI-MS/MS). The concentration of TA in the experimental turtles with respect to time was pharmacokinetically analyzed using a noncompartment model. The Cmax values of TA were 89.33 ± 6.99 µg/ml following i.m. administration. The elimination half-life values were 38.92 ± 6.31 hr and 41.09 ± 9.32 hr after i.v. and i.m. administration, respectively. The absolute i.m. bioavailability was 94.46%, and the average binding percentage of TA to plasma protein was 31.39%. TA demonstrated a long half-life and high bioavailability following i.m. administration. Therefore, the i.m. administration is recommended for use in clinical practice because it is both easier to perform and provides similar plasma concentrations to the i.v. administration. However, further studies are needed to determine the clinical efficacy of TA for treatment of inflammatory disease after single and multiple dosages.

Occurrence of Multiple Mycotoxins in Various Types of Rice and Barley Samples in Thailand.

HIGHLIGHTS: Contamination with multiple mycotoxins was found in rice and barley. BEA, DAS, ZEA, and aflatoxins were the mycotoxins most frequently found in samples. The assessed mycotoxin exposure does not represent a health risk for consumers.

Toxicokinetic profile of fusarenon-X and its metabolite nivalenol in the goat (Capra hircus).

The main aim of this research was to evaluate the toxicokinetic characteristics of fusarenon-X (FX) and its metabolite, nivalenol (NIV), in goats. The amounts of FX and NIV in post-mitochondrial (S-9), microsomal and cytosolic fractions of diverse tissues of the goat were also investigated. FX was intravenously (iv) or orally (po) administered to goats at dosages of 0.25 and 1 mg/kg bw, respectively. The concentrations of FX and NIV in plasma, feces and urine were quantified by liquid chromatography tandem-mass spectrometry (LC-ESI-MS/MS). The concentrations of FX in plasma were quantified up to 8 h with both routes of administration. A large amount of NIV (metabolite) was quantifiable in plasma, urine and feces after both administrations. The Cmax value of FX was 413.39 ±â€¯206.84 ng/ml after po administration. The elimination half-life values were 1.64 ±â€¯0.32 h and 4.69 ±â€¯1.25 h after iv and po administration, respectively. In vitro experiments showed that the conversion FX-to-NIV mainly occurs in the liver microsomal fraction. This is the first study that evaluates the fate and metabolism of FX in ruminant species.

Occurrence and Health Risk of Patulin and Pyrethroids in Fruit Juices Consumed in Bangkok, Thailand.

The mycotoxin patulin (PAT) is well known as a natural contaminant of apple- and other fruit-based products. Pesticides are a group of chemicals abundantly used in agriculture to maximize productivity by protecting crops from pests and weeds. Because of their harmful health effects, PAT and pesticides are strictly monitored. The current study was undertaken to investigate the significance of PAT and pyrethroid insecticide contamination in a variety of fruit juices in Bangkok. To do this, a total of 200 fruit juice samples, consisting of 40 samples each of apple, apricot, peach, pineapple, and grape juice, were collected from supermarkets in Bangkok, Thailand. PAT contamination in a variety of fruit juices was detected using validated liquid chromatography-tandem mass spectrometry, and pyrethroid insecticides (cypermethrin, cyfluthrin, and flumethrin) were analyzed using a gas chromatography equipped with micro-electron capture detector. The survey found that PAT concentrations were lower than the maximum residue limit established by European Union. The results of the present study suggest that the risk of exposure to harmful levels of PAT, cypermethrin, cyfluthrin, and flumethrin in fruit juices is very low in urban areas of Thailand.

Inhibitory Effects of Thai Essential Oils on Potentially Aflatoxigenic Aspergillus parasiticus and Aspergillus flavus.

　The antiaflatoxigenic and antifungal activities of essential oils (EOs) of finger root (Boesenbergia rotunda (L.) Mansf.), pine (Pinus pinaster), rosewood (Aniba rosaedora), Siam benzoin (Styrax tonkinensis), Thai moringa (Moringa oleifera), and ylang ylang (Cananga odorata) were tested for Aspergillus parasiticus and Aspergillus flavus in potato dextrose broth. Aflatoxin B1 (AFB1) was extracted from culture using a QuEChERS-based extraction procedure and analyzed with high performance liquid chromatography (HPLC) coupled to a fluorescence detector. EO of pine showed the greatest inhibition of growth and AFB1 production of A. parasiticus, followed by EOs of rosewood, finger root, Siam benzoin, and ylang ylang. EO of finger root gave the best inhibitory effects on A. flavus, followed by EOs of rosewood, pine, ylang ylang, and Siam benzoin. EO of Thai moringa did not show any significant inhibition of aflatoxigenic fungi. The antiaflatoxigenic activities of EOs correlated with their antifungal activities in the dosedependent manner. Comparison of the application of the five selected EOs in peanut pods by direct and vapor exposure indicated that the AFB1 production inhibitory effects of the five EOs by direct exposure were faster and more effective than by vapor exposure. EO of finger root showed the best inhibition of AFB1 production of A. flavus in peanut pods by direct exposure, followed by EOs of pine, rosewood, ylang ylang, and Siam benzoin.

Potential human health risk from consumption of metallic elements-contaminated benthic mollusks from Don Hoi Lot sandbar, Thailand.

Environmental pollutants have raised more concerns for human health risk, especially via consumption of contaminated food. Terrestrial as well as aquatic animals are capable of bioaccumulation a variety of toxic substances including metallic elements. With increasing anthropogenic activities along the coastal areas, living organisms have more chances to be exposed to released contaminants. In this study, seven metallic elements (Cd, Cu, Fe, Mn, Ni, Pb and Zn) were determined in sediments and water from Don Hoi Lot sandbar, Samutsongkharm province, Thailand. Potential human health risks via the consumption of two benthic bivalves Solen corneus (Larmarck, 1818) and Meretrix meretrix (Linnaeus, 1758) were also estimated using the target hazard quotients (THQs). The variations of metallic element concentrations were apparent between wet and dry season. Fe was the predominate metallic element in the sediment and the remaining were Mn>Pb>Zn>Ni>Cu>Cd. Whereas metallic element concentrations in water were Pb>Ni>Fe>Zn>Cu>Mn>Cd. PCA analysis confirmed that the contaminations of these metallic elements were from Mae Klong river surface water. Most Pb THQ values in both S. corneus and M. meretrix were >1 indicating that human health risk is of concern. However, the sum of THQs of an individual metallic element should also be considered since multiple metallic elements exposure is so common.

An overview of the toxicology and toxicokinetics of fusarenon-X, a type B trichothecene mycotoxin.

Fusarenon-X (FX) is a type B trichothecene mycotoxin that is frequently observed along with deoxynivalenol (DON) and nivalenol (NIV) in agricultural commodities. This review aims to give an overview of the literature concerning the toxicology and toxicokinetics of FX. FX is primarily found in cereals grown in temperate regions, but it can also be found worldwide because of the global transport of products. The major toxicity of FX occurs through inhibition of protein synthesis, followed by the disruption of DNA synthesis. Moreover, FX has also been shown to induce apoptosis in in vitro and in vivo studies. The targets of FX are organs containing actively proliferating cells, such as the thymus, spleen, skin, small intestine, testes and bone marrow. FX causes immunosuppression, intestinal malabsorption, developmental toxicity and genotoxicity. In addition, sufficient evidence of carcinogenicity in experimental animals is currently lacking, and the International Agency for Research on Cancer (IARC) classifies it as a group 3 carcinogen.

Survey of Deoxynivalenol and Aflatoxin B1 in Instant Noodles and Bread Consumed in Thailand by Using Liquid Chromatography-Tandem Mass Spectrometry.

One hundred wheat product samples (50 instant noodle samples and 50 bread samples) were collected from supermarkets in Bangkok, Thailand. Deoxynivalenol (DON) and aflatoxin B1 (AFB1) contamination in these products was analyzed using a validated liquid chromatography-tandem mass spectrometry method. The limit of quantification values of DON and AFB1 in the instant noodles and bread were 2 and 1 ng g(-1), respectively. The survey found that DON was quantifiable in 40% of collected samples, in 2% of noodles (0.089 µg g(-1)), and in 78% of breads (0.004 to 0.331 µg g(-1)). AFB1 was below the limit of quantification of the method in all of the tested samples. The results suggest that the risk of DON exposure via noodles and breads is very low in urban areas of Thailand. No risk can be attributable to AFB1 exposure in the same food matrices, but further studies with a larger sample size are needed to confirm these data.

Health risk associated with the consumption of duck egg containing endosulfan residues.

Eight duck farms and a local market in Suphanburi province, Thailand adjacent to paddy fields were selected for this study. The concentrations of endosulfan isomers (α- and ß-endosulfan) and endosulfan sulfate in environmental matrices (water, soil, feed) and duck eggs were determined. Human health risk via the contaminated egg consumption was also evaluated. Analysis of environmental matrices found both endosulfan isomers (α- and ß-endosulfan) and endosulfan sulfate in most samples. Endosulfan sulfate was predominantly found in all matrices followed by ß- and α-endosulfan, respectively. The total endosulfan concentrations were in the following order: feed > soil > water. However, the levels of endosulfan detected were lower than the regulatory maximum residue limit of endosulfan, except in water (>0.200 ng mL(-1)). Endosulfan sulfate in duck egg samples was also predominantly detected in both yolk and albumin. The average total endosulfan residues (∑endosulfan) in yolk (6.73 ng g(-1)) were higher than in albumin (4.78 ng g(-1)). According to principle component analysis, we found that paddy soil surrounding the duck farms is the suspected source of endosulfan contamination in husbandry water which subsequently contaminates duck eggs. The estimated daily intakes (EDIs) of these endosulfan-contaminated eggs were well below the acceptable daily intake (ADI) for endosulfan (6 µg kg(-1) day(-1)). However, the consumption of this contaminated duck eggs should be of concerns in regard to chronic exposure. Therefore, the better environmental managements to reduce endosulfan residues can play a crucial role for decreasing human health risk.

Using Nassarius stolatus as a potential heavy metal biomonitor.

Nassarius stolatus was evaluated as the potential heavy metal (Cd, Fe, Mn, Ni and Pb) accumulator in Don Hoi Lot sandbar, Samut Songkhram province, Thailand. This selected species belongs to the Gastropoda, which is widely distributed in the coastal areas from the upper Gulf through the southern part of Thailand. From our findings, the heavy metal accumulations in N. stolatus tissues were Fe > Pb > Mn > Ni > Cd. The retrieved bioaccumulation factor (BAF) indicated that N. Stolatus has high potential to be a biomonitor for the contaminations of Fe and Mn in water and Cd, Ni and Pb in sediment.

Oral exposure of fusarenon-X induced apoptosis in Peyer's patches, thymus, and spleen of mice.

ICR male mice were orally administered once daily with Fusarenon-X (FX) at 0, 0.1, 0.3, and 0.5mg/kg body weight for 14 days, and examined at 3, 6, 12, 24, and 48 h after last treatment (HAT) on Day 14. FX did not affect body and organ weight, however, at the higher doses FX caused nuclear condensation and fragmentation of lymphocytes in the cortical thymus and germinal center of Peyer's patches. Such effects were not observed in the liver, kidney and brain. Apoptotic lymphocytes evaluated by modified TUNEL method showed dose-dependency and peaked at 12 HAT in the Peyer's patches and thymus of 0.3 and 0.5mg/kg FX-treated mice, whereas apoptotic bodies engulfed by macrophage were clearly seen by electron microscopy in 0.5mg/kg FX-treated mice. Thus, repeated exposure to low doses of FX induces apoptosis in the lymphoid tissues of mice but did not affect liver, kidney and brain.

The Effects of Curcumin on Aflatoxin B1- Induced Toxicity in Rats.

To evaluate the potential of curcumin on toxic and carcinogenic effects of Aflatoxin B1 (AFB1) in relation to AFB1 metabolism, we studied the effects of curcumin on hepatic AFB1-DNA adduct formation and glutathione S-transferase (GST) activity, and the toxic effects of AFB1 in male Fischer 344 rats. Oral administration of curcumin to 5-week-old male rats at a dose of 8 or 80 mg/kg for five consecutive days for three weeks resulted in reduction of AFB1-DNA adduct formation mediated by both liver microsomal and postmitochondrial fractions. The activity of liver GST toward a universal substrate, CDNB, was increased in curcumin-administered rats. As for the acute toxicity of AFB1, curcumin was orally administered to rats for 3 weeks and then AFB1 was given by intragastric intubation. The result showed a decrease of plasma AST and ALT activities in curcumin-treated rats compared with those which received AFB1 alone. Moreover, we have observed that curcumin also reduced glutathione S-transferase placental form positive single cells and foci caused by AFB1 treatment. These results demonstrate the potential of curcumin to reduce the toxic and carcinogenic effects of AFB1 by modulating hepatic drug metabolizing enzymes responsible for AFB1 metabolism.

The fate and tissue disposition of deoxynivalenol in broiler chickens.

To evaluate the fate of deoxynivalenol (DON) in broilers, DON was administered either intravenously or orally to broilers at a dose of 1 mg/kg BW. Concentrations of DON in plasma were measurable up to 4 hr and 2 hr after intravenous and oral administration, respectively. Following intravenous administration, the values for the elimination half-life, the volume of distribution and the clearance were 1.25 ± 0.25 hr, 7.55 ± 2.03 l/kg and 4.16 ± 0.42 l/hr/kg, respectively. The oral bioavailability was 15.46 ± 4.02%. DON was detectable in all tissues examined after oral administration. These results suggest that DON is able to penetrate into the various tissues in broilers, though poorly absorbed from their gastrointestinal tract.

Dispositions and tissue residue of zearalenone and its metabolites α-zearalenol and ß-zearalenol in broilers.

Zearalenone (ZEA) is a secondary fungal metabolite produced mainly by a Fusarium graminearum. To clarify the toxicokinetics, and residues of ZEA and its major metabolites α-zearalenol (α-ZOL) and ß-zearalenol (ß-ZOL) in chickens, ZEA was then administered intravenously (iv) or orally (po) to broiler chickens at a dosage of 1.2 mg/kg body weight. The concentrations of ZEA, α-ZOL and ß-ZOL in the plasma and various tissues were quantified using LC-MS/MS. The plasma concentrations of ZEA were measurable up to 2 h after iv and po administration, and the concentrations of α-ZOL and ß-ZOL were detected up to 4 h after both types of administration. A two-compartment model was developed to describe the toxicokinetic of ZEA in broilers. The values of t1/2ß and Vd were 1.36 ± 0.29 h and 6.40 ± 0.89 l/kg, respectively. The absolute oral bioavailability was 29.66 ± 5.6%. ZEA, α-ZOL and ß-ZOL were measurable in the vital organs after po administration. These results suggest that ZEA is absorbed from the gastrointestinal tract and it has ability to penetrate into the various tissues of broiler chickens.

Distribution and residue depletion of oxytetracycline in giant freshwater prawn (Macrobrachium rosenbergii).

Distribution and residue depletion patterns of oxytetracycline were examined in giant freshwater prawns, Macrobrachium rosenbergii, after medicated-feed treatment at the feeding level of 4 g/kg of feed for 5 consecutive days. The concentration of oxytetracycline in prawn muscle tissues was measured using high-performance liquid chromatography equipped with a fluorescence detector. The limit of detection was 0.1 microg/g. In the medicated-feed-treated group, oxytetracycline was detectable 8 days posttreatment at the feeding level of 4 g/kg of feed for 5 consecutive days. The depletion half-life and mean residence time of oxytetracycline were 30.96 and 100.3 h, respectively. The dose of 4 g/kg of feed for 5 consecutive days can be recommended for therapeutic dosage regimen in giant freshwater prawn aquaculture. The minimal withdrawal period of M. rosenbergii muscle tissue should be 8 days postcessation of medicated feed.

PCB profiles in mouse skin biopsies and fat from an environmental mixture.

Fat, ear skin and trunk skin were collected from mice exposed to PCB-contaminated soil for PCB specific congener analysis. The soil had been retrieved from a Superfund site prior to remediation. Mice were exposed to either contaminated soil or control soil for 4 weeks. Skin samples were collected immediately after the 4-week exposure and again after 4-week recovery. Out of 141 PCB congeners measured, only 91 peaks representing 103 congeners were above quantification limits. Relative liver weights in PCB-exposed mice doubled indicating significant enzyme induction. Consistent with this observation, total PCB residues declined about 80% during the 4-week recovery. The PCB residue profiles in the ear skin (208mg/kg total) and trunk skin (129mg/kg) after 4-week exposure were identical and similar to those in body fat (370mg/kg) and in the contaminated soil. The results confirmed that both skin tissues are important reservoirs and can serve as useful biopsy media.