Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros








Base de dados
Intervalo de ano de publicação
1.
Klin Lab Diagn ; 59(10): 40-5, 2014 Oct.
Artigo em Russo | MEDLINE | ID: mdl-25884079

RESUMO

To decrease dependence of effectiveness of isolation of nucleic acids of composition and amount of applied sample a kit was developed for hybridization extraction of DNA HBV RNA HCV and RNA HIV from blood serum in two formats--using up to 250 mkl and up to 1 ml of sample. This kit, in complex with kits for detection using polymerase chain reaction technique in real-time, forms a test characterized by high analytical sensitivity i.e. HBV50 copies per ml, HCV37.5 copies per ml, HIV 13 copies per ml. The developed kit for extraction of target nucleic acids permits to get rid of total DNA and inhibited effect of heparin. It can be adapted for application wit factors B and automated stations of sample preparation.


Assuntos
DNA Viral , Infecções por HIV/sangue , Hepatite B/sangue , Hepatite C/sangue , Kit de Reagentes para Diagnóstico , DNA Viral/sangue , DNA Viral/isolamento & purificação , HIV-1 , Hepacivirus , Vírus da Hepatite B , Humanos , RNA Viral/sangue , RNA Viral/isolamento & purificação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA