Anti-UV Microgel Based on Interfacial Polymerization to Decrease Skin Irritation of High Permeability UV Absorber Ethylhexyl Methoxycinnamate.

Ethylhexyl methoxycinnamate (EHMC) is frequently employed as a photoprotective agent in sunscreen formulations. EHMC has been found to potentially contribute to health complications as a result of its propensity to produce irritation and permeate the skin. A microgel carrier, consisting of poly(ethylene glycol dimethacrylate) (pEDGMA), was synthesized using interfacial polymerization with the aim of reducing the irritation and penetration of EHMC. The thermogravimetric analysis (TGA) indicated that the EHMC content accounted for 75.72% of the total composition. Additionally, the scanning electron microscopy (SEM) images depicted the microgel as exhibiting a spherical morphology. In this study, the loading of EHMC was demonstrated through FTIR and contact angle tests. The UV resistance, penetration, and skin irritation of the EHMC-pEDGMA microgel were additionally assessed. The investigation revealed that the novel sunscreen compound, characterized by limited dermal absorption, had no irritant effects and offered sufficient protection against ultraviolet radiation.

Virome diversity shaped by genetic evolution and ecological landscape of Haemaphysalis longicornis.

BACKGROUND: Haemaphysalis longicornis is drawing attentions for its geographic invasion, extending population, and emerging disease threat. However, there are still substantial gaps in our knowledge of viral composition in relation to genetic diversity of H. longicornis and ecological factors, which are important for us to understand interactions between virus and vector, as well as between vector and ecological elements. RESULTS: We conducted the meta-transcriptomic sequencing of 136 pools of H. longicornis and identified 508 RNA viruses of 48 viral species, 22 of which have never been reported. Phylogenetic analysis of mitochondrion sequences divided the ticks into two genetic clades, each of which was geographically clustered and significantly associated with ecological factors, including altitude, precipitation, and normalized difference vegetation index. The two clades showed significant difference in virome diversity and shared about one fifth number of viral species that might have evolved to "generalists." Notably, Bandavirus dabieense, the pathogen of severe fever with thrombocytopenia syndrome was only detected in ticks of clade 1, and half number of clade 2-specific viruses were aquatic-animal-associated. CONCLUSIONS: These findings highlight that the virome diversity is shaped by internal genetic evolution and external ecological landscape of H. longicornis and provide the new foundation for promoting the studies on virus-vector-ecology interaction and eventually for evaluating the risk of H. longicornis for transmitting the viruses to humans and animals. Video Abstract.

Histone methylation readers MRG1/2 interact with PIF4 to promote thermomorphogenesis in Arabidopsis.

Warm ambient conditions induce thermomorphogenesis and affect plant growth and development. However, the chromatin regulatory mechanisms involved in thermomorphogenesis remain largely obscure. In this study, we show that the histone methylation readers MORF-related gene 1 and 2 (MRG1/2) are required to promote hypocotyl elongation in response to warm ambient conditions. A transcriptome sequencing analysis indicates that MRG1/2 and phytochrome interacting factor 4 (PIF4) coactivate a number of thermoresponsive genes, including YUCCA8, which encodes a rate-limiting enzyme in the auxin biosynthesis pathway. Additionally, MRG2 physically interacts with PIF4 to bind to thermoresponsive genes and enhances the H4K5 acetylation of the chromatin of target genes in a PIF4-dependent manner. Furthermore, MRG2 competes with phyB for binding to PIF4 and stabilizes PIF4 in planta. Our study indicates that MRG1/2 activate thermoresponsive genes by inducing histone acetylation and stabilizing PIF4 in Arabidopsis.

The complete mitogenome of Argas vulgaris (Filippova, 1961) and its phylogenetic status in subgenus Argas (Acari: Argasidae).

Soft ticks (Ixodida: Argasidae) are ectoparasites of terrestrial vertebrates with worldwide distributions. As one representative group of Argasidae, the genus Argas has an important vectorial role in transmitting zoonotic diseases. However, our knowledge of the subgenus Argas in China is still limited, as most literature only lists occurrence records or describes specific case reports without providing detailed morphological characteristics and further molecular data. This study aims to characterize Argas vulgaris through complete mitochondrial sequencing and morphological diagnostic techniques based on a batch of adult specimens collected from Ningxia Hui Autonomous Regions (NXHAR), North China. The morphology and microstructures of Ar. vulgaris and other lectotypes of argasid ticks in the subgenus Argas were also observed using a stereomicroscope. Following DNA extraction and sequencing, a complete mitochondrial sequence of Ar. vulgaris was assembled and analyzed within a phylogenetic context. The 14,479 bp mitogenome of Ar. vulgaris consists of 37 genes, including 13 genes for protein coding, two for ribosomal RNA, 22 for transfer RNA, and one for control region (D-loops). Phylogenetic analysis of Ar. vulgaris showed 98.27%-100% nucleotide identity with Ar. japonicus, indicating a close relationship between the two tick species. The morphological diagnostic features to differentiate Ar. vulgaris from other ticks within the subgenus Argas included the location of the anus and setae on the anterior lip of the female genital aperture. This study provided high-resolution scanning electron microscope images of female Ar. vulgaris and corresponding molecular data, representing valuable resources for future accurate species identification.

Investigating the pathogens associated with Dermacentor nuttalli and its global distribution: A study integrating metagenomic sequencing, meta-analysis and niche modeling.

Dermacentor nuttalli, a member of family Ixodidae and genus Dermacentor, is predominantly found in North Asia. It transmits various pathogens of human and animal diseases, such as Lymphocytic choriomeningitis mammarenavirus and Brucella ovis, leading to severe symptoms in patients and posing serious hazards to livestock husbandry. To profile pathogen abundances of wild D. nuttalli, metagenomic sequencing was performed of four field-collected tick samples, revealing that Rickettsia, Streptomyces, and Pseudomonas were the most abundant bacterial genera in D. nuttalli. Specifically, four nearly complete Rickettsia genomes were assembled, closely relative to Rickettsia conorii subsp. raoultii. Then, a comprehensive meta-analysis was performed to evaluate its potential threats based on detected pathogens and geographical distribution positions reported in literature, reference books, related websites, and field surveys. At least 48 pathogens were identified, including 20 species of bacteria, seven species of eukaryota, and 21 species of virus. Notably, Rickettsia conorii subsp. raoultii, Coxiella burnetii, and Brucella ovis displayed remarkably high positivity rates, which were known to cause infectious diseases in both humans and livestock. Currently, the primary distribution of D. nuttalli spans China, Mongolia, and Russia. However, an additional 14 countries in Asia and America that may also be affected by D. nuttalli were identified in our niche model, despite no previous reports of its presence in these areas. This study provides comprehensive data and analysis on the pathogens carried by D. nuttalli, along with documented and potential distribution, suggesting an emerging threat to public health and animal husbandry. Therefore, there is a need for heightened surveillance and thorough investigation of D. nuttalli.

Anion Recognition in Solution: Insights from Thermodynamics and Ultrafast Structural Dynamics.

Anion recognition through noncovalent interactions stands as an emerging field in supramolecular chemistry, exerting a profound influence on the regulation of biological functions. Herein, the thermodynamics of complexation between sodium cyanate (NaOCN) and calix[4]pyrrole was systematically investigated by linear and nonlinear IR spectroscopy, highlighting enthalpy changes as the dominant driving force. The overall orientational relaxation of bound anion can be described by an Arrhenius-type activated process, yielding an activation energy of 15.0 ± 1.0 kJ mol-1. The structural dynamics of contact ion pairs (CIPs) formed between Na+ and OCN- in solution showed a negligible temperature effect, suggesting entropy changes as the principal governing factor. Further analysis revealed that anion recognition in solution is mediated by conformational changes of the receptor and collective rearrangement of hydrogen bond dynamics. This study, framed within the paradigms of thermodynamics and ultrafast structural dynamics, substantially advances our comprehension of the microscopic mechanisms underlying anion recognition in the realm of supramolecular chemistry.

Systematic review and integrated data analysis reveal diverse pangolin-associated microbes with infection potential.

There has been increasing global concern about the spillover transmission of pangolin-associated microbes. To assess the risk of these microbes for emergence as human pathogens, we integrated data from multiple sources to describe the distribution and spectrum of microbes harbored by pangolins. Wild and trafficked pangolins have been mainly recorded in Asia and Africa, while captive pangolins have been reported in European and North American countries. A total of 128 microbes, including 92 viruses, 25 bacteria, eight protists, and three uncharacterized microbes, have been identified in five pangolin species. Out of 128 pangolin-associated microbes, 31 (including 13 viruses, 15 bacteria, and three protists) have been reported in humans, and 54 are animal-associated viruses. The phylogenetic analysis of human-associated viruses carried by pangolins reveals that they are genetically close to those naturally circulating among human populations in the world. Pangolins harbor diverse microbes, many of which have been previously reported in humans and animals. Abundant viruses initially detected in pangolins might exhibit risks for spillover transmission.

Impact of green digital finance on green economic recovery and green agricultural development: implications for green environment.

This study explores the potential of green digital finance and green agricultural growth to contribute to the green economic recovery of the BRICS economies. We examine the relationship between these variables using empirical data and various statistical techniques, including vector error correction, co-integration, unit root tests and long-run analysis. Our results suggest that well-functioning financial institutions play a crucial role in facilitating the structural transformation of green digital finance and promoting green agricultural growth to achieve green economic recovery in the BRICS region. Our findings underscore the need for pro-financial and green economic development policies and institutions to support and enhance economic recovery. Our results are robust and supported by our study. We also suggest future research directions for stakeholders interested in promoting sustainable economic growth in the BRICS countries.

Association between negatively perceived parenting attitudes and dissociation: a cross-sectional study on the general population in Japan.

Objectives: Many studies have reported that early traumatic experiences, mainly abuse, are associated with forming dangerous attachments and a contributing factor to dissociation. On the other hand, other studies have investigated the association of non-abusive nurturing and attachment styles with dissociation. The aim of this study is to determine the frequency of dissociative experiences in the general Japanese population and investigate the effects of "overprotection" and "lack of care" as nurturing styles and "abandonment anxiety" and "avoidance of intimacy" as attachment styles on dissociation. Methods: A total of 1,042 residents aged 18 to 69 years were administered with the Dissociative Experiences Scale (DES), the Japanese version of the WHO-5 Well-Being Index (WHO-5-J), the Parental Bonding Instrument (PBI), and the Experiences in Close Relationships Inventory-the-generalized-other-version (ECR-GO). Hierarchical multiple regression analyses on the effects of "overprotection" and "care" as nurturing attitudes "abandonment anxiety" and "avoidance of intimacy" as attachment styles on dissociation (DES-NDI and DES-T) were conducted. Results: Based on the findings of this study, "care" and "overprotection" as nurturing attitudes were shown to be a contributing factor to dissociation (DES-NDI and DES-T). "Avoidance of intimacy" as an attachment style was shown to contribute to pathological dissociation. On the other hand, the influence of attachment style on the relationship between nurturing style and dissociation was not determined. Discussion: This study provided essential data on the distribution of dissociative experiences in the general Japanese population. It was indicated that nurturing style, particularly overprotection, may be linked to nonfunctional stress coping and interpersonal anxiety and could be a contributing factor to mental disorders, including dissociation. Furthermore, considering that the effect of nurturing styles on dissociation does not vary with attachment styles, the effect of nurturing styles on dissociation may be more profound.

Genomic Characteristics of Emerging Intraerythrocytic Anaplasma capra and High Prevalence in Goats, China.

Anaplasma capra is an emerging tickborne human pathogen initially recognized in China in 2015; it has been reported in ticks and in a wide range of domestic and wild animals worldwide. We describe whole-genome sequences of 2 A. capra strains from metagenomic sequencing of purified erythrocytes from infected goats in China. The genome of A. capra was the smallest among members of the genus Anaplasma. The genomes of the 2 A. capra strains contained comparable G+C content and numbers of pseudogenes with intraerythrocytic Anaplasma species. The 2 A. capra strains had 54 unique genes. The prevalence of A. capra was high among goats in the 2 endemic areas. Phylogenetic analyses revealed that the A. capra strains detected in this study were basically classified into 2 subclusters with those previously detected in Asia. Our findings clarify details of the genomic characteristics of A. capra and shed light on its genetic diversity.

Influence of posttraumatic stress reaction and posttraumatic cognition on dissociative experiences according to the nature of traumatic experiences: A survey on Japanese young adults.

OBJECTIVES: Although the relationship between dissociation and traumatic experiences has been debated, many studies focus on the context of traumatic experiences. Alternatively, basic research that examines the relationship of symptoms of posttraumatic experiences and cognitive characteristics of dissociation as an individual difference among experiencers has been lacking. To address this research gap, this study examines the influence of posttraumatic symptoms and cognition on dissociative experiences given the nature of traumatic experiences. METHOD: This study administered the Events Checklist, the Dissociative Experiences Scale (DES), the Impact of Event Scale-Revised (IES-R), and the Japanese version of the Posttraumatic Cognitions Inventory to 1,036 Japanese adolescents. It investigated differences in dissociative experiences according to the presence of and cumulative traumatic experiences. Hierarchical multiple regression analysis was conducted to determine the influence of sex and the nature of traumatic experiences, posttraumatic symptoms (IES-R), and cognition on dissociative experiences. RESULTS: The results demonstrated no differences in DES based on the number of traumatic experiences. Moreover, this study observed the impact of cognition in posttraumatic experience on dissociation. CONCLUSIONS: This study provides essential data on the distribution of dissociative experiences among Japanese adolescents with traumatic experiences and exposure to adversity. Finally, it highlights the importance of focusing on posttraumatic cognitive characteristics, particularly negative self-perception, and discusses the implications to enhance understanding of dissociative experiences. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Determination of uric acid in serum by SERS system based on VO-MnCo2O4/Ag nanozyme.

The level of uric acid is crucial to human health. Octahedral oxygen vacancy MnCo2O4/Ag (VO-MnCo2O4/Ag) nanozyme was successfully prepared by simple hydrothermal, calcination and self-reduction methods. VO-MnCo2O4/Ag nanozyme is rich in Mn2+/Mn3+ and CO2+/CO3+ redox electron pairs, large specific surface area and oxygen vacancies. VO-MnCo2O4/Ag nanozyme showed high uricase-like activity and peroxidase-like activity. At the same time, the SERS signal of the detected molecule could be significantly enhanced after the catalytic reaction of the VO-MnCo2O4/Ag nanozyme. The Km values of VO-MnCo2O4/Ag nanozyme for H2O2 and TMB were 0.04 mM and 0.027 mM respectively. Based on the uric acid oxidase-like and peroxidase-like activities of VO-MnCo2O4/Ag, we developed a label-free, sensitive, and reliable SERS uric acid detection system. The detection linear range of uric acid is 0.01 µM-1000 µM and the detection of limit is 7.8 × 10-9 M. The results show that the sensing system has good accuracy, sensitivity, selectivity, and stability. It can be applied to the determination of samples under different conditions. This study provides profound insights into the design of enzyme-like activity regulation and SERS properties regulation of nanozymes, provides guidance for the study of reaction kinetics and catalytic mechanism of nanozymes, and has broad application prospects in the field of nanozymes and SERS sensing analysis.

Genomic Characterization of Theileria luwenshuni Strain Cheeloo.

Theileria, a tick-borne intracellular protozoan, can cause infections of various livestock and wildlife around the world, posing a threat to veterinary health. Although more and more Theileria species have been identified, genomes have been available only from four Theileria species to date. Here, we assembled a whole genome of Theileria luwenshuni, an emerging Theileria, through next-generation sequencing of purified erythrocytes from the blood of a naturally infected goat. We designated it T. luwenshuni str. Cheeloo because its genome was assembled by the researchers at Cheeloo College of Medicine, Shandong University, China. The genome of T. lunwenshuni str. Cheeloo was the smallest in comparison with the other four Theileria species. T. luwenshuni str. Cheeloo possessed the fewest gene gains and gene family expansion. The protein count of each category was always comparable between T. luwenshuni str. Cheeloo and T. orientalis str. Shintoku in the Eukaryote Orthologs annotation, though there were remarkable differences in genome size. T. luwenshuni str. Cheeloo had lower counts than the other four Theileria species in most categories at level 3 of Gene Ontology annotation. Kyoto Encyclopedia of Genes and Genomes annotation revealed a loss of the c-Myb in T. luwenshuni str. Cheeloo. The infection rate of T. luwenshuni str. Cheeloo was up to 81.5% in a total of 54 goats from three flocks. The phylogenetic analyses based on both 18S rRNA and cox1 genes indicated that T. luwenshuni had relatively low diversity. The first characterization of the T. luwenshuni genome will promote better understanding of the emerging Theileria. IMPORTANCE Theileria has led to substantial economic losses in animal husbandry. Whole-genome sequencing data of the genus Theileria are currently limited, which has prohibited us from further understanding their molecular features. This work depicted whole-genome sequences of T. luwenshuni str. Cheeloo, an emerging Theileria species, and reported a high prevalence of T. luwenshuni str. Cheeloo infection in goats. The first assembly and characterization of T. luwenshuni genome will benefit exploring the infective and pathogenic mechanisms of the emerging Theileria to provide scientific basis for future control strategies of theileriosis.

A novel S,N-rich MOF for efficient recovery of Au(III): Performance and mechanism.

A novel S,N-rich MOF with adenine and 4,4'-thiodiphenol as organic ligands was synthesized via the one-step solvothermal method, and used for gold recovery. The pH impact, adsorption kinetics, isotherms, thermodynamics, selectivity, and reusability were investigated accordingly. The adsorption and desorption mechanism were also explored comprehensively. The electronic attraction, coordination, and in situ redox account for the Au(III) adsorption. The Au(III) adsorption is affected strongly by the pH of solutions, and best at pH of 2.57. The MOF exhibits exceptional adsorption capacity as high as 3680 mg/g at 55 °C, fast kinetics with 8 min for 9.6 mg/L Au(III), and excellent selectivity for gold ion in real e-waste leachates. The adsorption process of gold on the adsorbent is endothermic and spontaneous, and influenced visibly by temperature. The adsorption ratio still maintained 99% after seven adsorption-desorption cycles. The column adsorption experiments show that the MOF has outstanding selectivity for Au(III) with 100% of removal efficiency in a complex solution containing Au, Ni, Cu, Cd, Co, and Zn ions. A glorious adsorption with a breakthrough time of 532 mins was obtained for the breakthrough curve. This study not only provides an efficient adsorbent for gold recovery, but also guidance for designing new materials.

Effects of Tai Chi on Sleep Quality as Well as Depression and Anxiety in Insomnia Patients: A Meta-Analysis of Randomized Controlled Trials.

To systematically review the effects of Tai chi on sleep quality, depression, and anxiety in patients with insomnia. The electronic databases including PubMed, Cochrane Library, Web of Science, Embase, China National Knowledge Infrastructure (CNKI), WanFang Data, Chinese Biomedical Literature Database (CBM), and VIP Database for Chinese Technical Periodicals (VIP) were retrieved and screened by computer. Randomized controlled trials (RCT) on patients with insomnia who practiced Tai chi were collected, and the RCT risk of bias assessment criteria was used to evaluate the methodological quality of the included studies. The combined effect size was expressed as the weighted mean difference (WMD), with a confidence interval of 95% (CI). Review Manager 5.4 and Stata16.0 were used for heterogeneity analysis and sensitivity analysis. Tai chi reduced the patients' Pittsburgh sleep quality index (PSQI) score (WMD = -1.75, 95% CI: -1.88, -1.62, p < 0.001); Hamilton depression scale (HAMD) score (WMD = -5.08, 95% CI: -5.46, -4.69, p < 0.001), Hamilton anxiety scale (HAMA) score (WMD = -2.18, 95% CI: -2.98, -1.37, p < 0.001), and self-rating anxiety scale (SAS) score (WMD = -7.01, 95% CI: -7.72, -6.29, p < 0.001). Tai chi exercise has a good preventive and ameliorating effect on insomnia, which can relieve patients' depression and anxiety, simultaneously enhancing various functions of the body. However, most of the included studies reported random assignment with some lack of specific descriptions, and the blinding of participants was difficult to achieve due to the nature of exercise, which may cause bias. Therefore, more high-quality, multi-center, and bigger-sample studies need to be included in the future to further verify the results.

Novel ultrasensitive Raman assay method based on enzyme mimetics for ultra trace of H2O2.

Enzyme mimetics have been widely applied on H2O2 assay, but it is still challenging and interesting to realize the sensitive detection for ultra-trace H2O2. Here, an ultrasensitive Raman assay method based on novel WO3@IP6-Fe3+ enzyme mimetics with peroxidase-like activity was established. WO3 microspheres (MSs) were found to have weak peroxidase-like activity, and the combination of IP6-Fe3+ and WO3 can produce stronger activity. WO3@IP6-Fe3+ MSs showed polyhedron-like structure, uniform size, and smooth surface. Although WO3@IP6-Fe3+ enzyme mimetics have low catalytic efficiency and high absorbance background, the proposed Raman method can bypass the above problems. In Raman method, high concentration of WO3@IP6-Fe3+ can be used to overcome low catalytic efficiency without high absorbance background. Moreover, 3,3',5,5'-tetramethylbenzidine oxide has prominent characteristic Raman peak at 1608 cm-1, greatly improving the sensitivity and eliminating interference of impurities. Due to the high sensitivity and low background, Raman assay showed the ultra-low limit of detection (5.49 × 10-15 M), which was 4-7 orders of magnitude lower than other detection methods. The ultrasensitive Raman assay not only provided the possibility for the enzyme mimetics-based detection of ultra-trace H2O2, but also enable the enzyme mimetics with low activity to be applied.

Policy Analysis of the Integration of Sports and Medicine against the Backdrop of "Healthy China": A Qualitative Study Using NVivo.

In China, the aim of integrating sports and medicine is part of a national health promotion policy. It is important to clarify the relevant policy points, policy practice distribution, and practical tools, as well as to find the weak links in the policy. In the study, there are 34 primary child nodes, 12 secondary child nodes and four parent nodes that were formed. In this study NVivo 11 software was used to analyze the content of 15 national guidelines in terms of integrating sports and medicine. From 2014 to 2021, the policy development of the integration of sports and medicine went through the beginning and growth stages. The evolutionary logic presents an inverse relationship between the policy practice's duration and the degree of state intervention. In the sequential developmental phases, policy tools were set up in an orderly transition from a single mandatory policy tool to a voluntary or hybrid policy tool, supplemented by essential policy tools. With respect to the policy content, the attention to specific service groups and sports risk assessment is insufficient. In the future, we should actively focus on the division of particular service groups and their service supply, pay closer attention to the social needs and value manifestation of sports risk assessment, and balance the proportion of policy tools in the development of the integration of sports and medicine.

The effects of biochar derived from feedstock with different Si and Al concentration on soil N2O and CO2 emissions.

Desilicification and allitization is important characteristic of acidic soil. While decrease in soil silicon (Si) may generate Si limitation, the increase of aluminum (Al) will aggravate soil acidification. Biochar has been used in acid soil improvement, which could mitigate nitrous oxide (N2O) emissions and alter soil Si and Al concentration. However, the effect of biochar with different Si and Al concentration on greenhouse gas emissions remains unclear. We evaluated the effects of biochar derived from feedstock with different Si (moso bamboo leaves, BL; rice straw, RS) and Al (Camellia oleifera fruit shell, CFS; C. oleifera leaves, CL) concentration on greenhouse gas emissions and soil acidification. Microbial functional gene abundance associated with N2O emissions were measured to further explore the response of microbiological community. The results showed that BL, RS, CFS and CL significantly increased soil pH (by 19.2%, 16.7%, 18.7% and 24.9%, respectively), decreased soil exchangeable acid and exchangeable Al content, and reduced N2O emission rate of soil with nitrogen (N) (by 14.2%, 27.3%, 25.6% and 38.7%, respectively), which correlated with increase in soil nosZ abundance. BL, RS, CFS and CL increased soil nirK (by 325.6%, 66.7%, 155.8%, and 253.2%, respectively) and nosZ (by 198.6%, 174.1%, 72.2%, and 152.0%, respectively) abundance with N. Structural equation model showed that Si input via biochar application directly reduced N2O emissions, and soil acid-extractable Si is inversely proportional to N2O emission rate. In addition, Si input reduced carbon dioxide (CO2) emissions via indirect effects. Al input via biochar addition indirectly affected N2O and CO2 emissions through mainly indirect effects on other soil factors. In intensive management and production activities, Si-rich biochar can be considered instead of sole addition as fertilizer, which will be beneficial to the sustainable development of agricultural and forestry production in acid soil areas, and mitigation of global change.

Self-assembled Cr2O3@nanogel/Au nanozymes to simulate peroxidase activity as a H2O2 sensor.

The low temperature solvothermal method synthesized Cr2O3 NPs has not only peroxidase activity, but also oxidase activity. Then, the oxidase activity of Cr2O3 NPs is effectively shielded by nanogel immobilization using three monomers acrylamide, NIPAAM (N-isopropylacrylamide) and MBA (N,N'-methylene bisacrylamide) in HEPES (4-(2-hydroxyerhyl)piperazine-1-erhanesulfonic acid) buffer. Ultimately, the enzymatic activity of Cr2O3@nanogel/Au is significantly enhanced after doping Au NPs by SERS (Surface Enhanced Raman Spectroscopy) evaluation. A SERS strategy was proposed for the detection of H2O2 by Cr2O3@nanogel/Au. The linear range was 10-8 mol·L-1-10-1 mol·L-1.