RESUMO
Rapid, efficient, versatile, easy-to-use, and non-expensive analytical approaches are globally demanded for food analysis. Many ambient ionization approaches based on electrospray ionization (ESI) have been developed recently for the rapid molecular characterization of food products. However, those approaches mainly suffer from insufficient signal duration for comprehensive chemical characterization by tandem MS analysis. Here, a commercially available disposable gel loading tip is used as a low-cost emitter for the direct ionization of untreated food samples. The most important advantages of our approach include high stability, and durability of the signal (> 10 min), low cost (ca. 0.1 USD per run), low sample and solvent consumption, prevention of tip clogging and discharge, operational simplicity, and potential for automation. Quantitative analysis of sulfapyridine, HMF (hydroxymethylfurfural), and chloramphenicol in real sample shows the limit-of-detection 0.1 µg mL-1, 0.005 µg mL-1, 0.01 µg mL-1; the linearity range 0.1-5 µg mL-1, 0.005-0.25 µg mL-1, 0.01-1 µg mL-1; and the linear fits R2 ≥ 0.980, 0.991, 0.986. Moreover, we show that tip-ESI can also afford sequential molecular ionization of untreated viscous samples, which is difficult to achieve by conventional ESI. We conclude that tip-ESI-MS is a versatile analytical approach for the rapid chemical analysis of untreated food samples.
Assuntos
Análise de Alimentos , Limite de Detecção , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas por Ionização por Electrospray/métodos , Análise de Alimentos/métodos , Géis/química , Cloranfenicol/análise , Contaminação de Alimentos/análise , Furaldeído/análise , Furaldeído/análogos & derivadosRESUMO
Electrospun (ES) fibrous nanomaterials have been widely investigated as novel biomaterials. These biomaterials have to be safe and biocompatible; hence, they need to be tested for cytotoxicity before being administered to patients. The aim of this study was to develop a suitable and biorelevant in vitro cytotoxicity assay for ES biomaterials (e.g. wound dressings). We compared different in vitro cytotoxicity assays, and our model wound dressing was made from polycaprolactone and polyethylene oxide and contained chloramphenicol as the active pharmaceutical ingredient. Baby Hamster Kidney cells (BHK-21), human primary fibroblasts and MTS assays together with real-time cell analysis were selected. The extract exposure and direct contact safety evaluation setups were tested together with microscopic techniques. We found that while extract exposure assays are suitable for the initial testing, the biocompatibility of the biomaterial is revealed in in vitro direct contact assays where cell interactions with the ES wound dressing are evaluated. We observed significant differences in the experimental outcome, caused by the experimental set up modification such as cell line choice, cell medium and controls used, conducting the phosphate buffer washing step or not. A more detailed technical protocol for the in vitro cytotoxicity assessment of ES wound dressings was developed.
Assuntos
Bandagens , Materiais Biocompatíveis , Cicatrização , Animais , Cicatrização/efeitos dos fármacos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Humanos , Linhagem Celular , Teste de Materiais , Cricetinae , Poliésteres/química , Fibroblastos/efeitos dos fármacos , Anti-Infecciosos/farmacologia , Polietilenoglicóis/química , Cloranfenicol/farmacologiaRESUMO
Limited literature exists on chloramphenicol's clinical use. In this retrospective, single-center case-series, we examined 183 chloramphenicol-treated and 81 piperacillin-tazobactam-treated medical patients. Chloramphenicol recipients were older, more debilitated, cognitively impaired, and penicillin allergic, while increased need for inotropics, higher leukocyte count, and higher creatinine levels were notable in the piperacillin-tazobactam group. Pneumonia was the most common indication, with no mortality difference between groups. While acknowledging its antimicrobial activity and potential benefit in specific conditions such as pneumonia, further clinical studies are needed to assess the role of chloramphenicol in the setting where other alternatives are available.
Assuntos
Antibacterianos , Cloranfenicol , Humanos , Cloranfenicol/uso terapêutico , Estudos Retrospectivos , Antibacterianos/uso terapêutico , Masculino , Idoso , Feminino , Pessoa de Meia-Idade , Idoso de 80 Anos ou mais , Hospitalização/estatística & dados numéricos , Resultado do Tratamento , Combinação Piperacilina e Tazobactam/uso terapêutico , Adulto , DemografiaRESUMO
In the present work, we developed an aptasensor to determine chloramphenicol (CAP) based on the dual signal output of photoelectrochemistry (PEC) and colorimetry. The Fe3+-doped porous tungsten trioxide was prepared by sol-gel method and coated on the ITO conductive glass to form ITO/p-W(Fe)O3. After assembling the captured DNA (cDNA) and the aptamer of CAP (apt) successively, the constructed ITO/p-W(Fe)O3-cDNA/apt aptasensor exhibited excellent photocurrent response under visible light irradiation in the presence of glucose, which provided the feasibility for PEC measurement with high sensitivity. In the presence of CAP, the apt left the ITO/p-W(Fe)O3 surface and AuNPs linked on the probe DNA would be assembled on it, which led to the decrease of photocurrent. Thanks to the oxidase-mimic catalytic performance of AuNPs and the recycling catalytic hydrolysis by exonuclease I, the measurement signal of the aptasensor could be amplified significantly, and the photocurrent decrease of the aptasensor was linearly related to the concentration of CAP in the range of 1.0 pM-10.0 nM and low detection limit was 0.36 pM. Meanwhile, the H2O2 produced from catalytic oxidation of glucose could oxidize TMB to blue oxTMB under HRP catalysis, which absorbance at 652 nm was linearly related to the concentration of CAP in the range of 5.0 pM-10.0 nM and low detection limit was 1.72 pM. Therefore, an aptasensor that determine CAP in real samples was successfully constructed with good precision of the relative standard deviation less than 5.7 % for PEC method and 7.3 % for colorimetric method, which can meet the analysis needs in different scenarios.
Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Cloranfenicol , Colorimetria , Técnicas Eletroquímicas , Ouro , Nanopartículas Metálicas , Cloranfenicol/análise , Cloranfenicol/química , Aptâmeros de Nucleotídeos/química , Colorimetria/métodos , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Ouro/química , Nanopartículas Metálicas/química , Limite de Detecção , Óxidos/química , Processos Fotoquímicos , Antibacterianos/análise , Antibacterianos/química , TungstênioRESUMO
A decreased chloramphenicol susceptibility in Haemophilus influenzae is commonly caused by the activity of chloramphenicol acetyltransferases (CATs). However, the involvement of membrane proteins in chloramphenicol susceptibility in H. influenzae remains unclear. In this study, chloramphenicol susceptibility testing, whole-genome sequencing, and analyses of membrane-related genes were performed in 51 H. influenzae isolates. Functional complementation assays and structure-based protein analyses were conducted to assess the effect of proteins with sequence substitutions on the minimum inhibitory concentration (MIC) of chloramphenicol in CAT-negative H. influenzae isolates. Six isolates were resistant to chloramphenicol and positive for type A-2 CATs. Of these isolates, A3256 had a similar level of CAT activity but a higher chloramphenicol MIC relative to the other resistant isolates; it also had 163 specific variations in 58 membrane genes. Regarding the CAT-negative isolates, logistic regression and receiver operator characteristic curve analyses revealed that 48T > G (Asn16Lys), 85 C > T (Leu29Phe), and 88 C > A (Leu30Ile) in HI_0898 (emrA), and 86T > G (Phe29Cys) and 141T > A (Ser47Arg) in HI_1177 (artM) were associated with enhanced chloramphenicol susceptibility, whereas 997G > A (Val333Ile) in HI_1612 (hmrM) was associated with reduced chloramphenicol susceptibility. Furthermore, the chloramphenicol MIC was lower in the CAT-negative isolates with EmrA-Leu29Phe/Leu30Ile or ArtM-Ser47Arg substitution and higher in those with HmrM-Val333Ile substitution, relative to their counterparts. The Val333Ile substitution was associated with enhanced HmrM protein stability and flexibility and increased chloramphenicol MICs in CAT-negative H. influenzae isolates. In conclusion, the substitution in H. influenzae multidrug efflux pump HmrM associated with reduced chloramphenicol susceptibility was characterised.
Assuntos
Substituição de Aminoácidos , Antibacterianos , Proteínas de Bactérias , Cloranfenicol O-Acetiltransferase , Cloranfenicol , Haemophilus influenzae , Testes de Sensibilidade Microbiana , Cloranfenicol/farmacologia , Haemophilus influenzae/genética , Haemophilus influenzae/efeitos dos fármacos , Haemophilus influenzae/metabolismo , Haemophilus influenzae/isolamento & purificação , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Cloranfenicol O-Acetiltransferase/genética , Cloranfenicol O-Acetiltransferase/metabolismo , Farmacorresistência Bacteriana Múltipla/genética , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/metabolismo , Resistência ao Cloranfenicol/genética , Humanos , Infecções por Haemophilus/microbiologia , Sequenciamento Completo do GenomaRESUMO
Despite the beneficial effects of antibiotics such as chloramphenicol (CAP), they exert some destructive impacts on human health. We designed an electrochemical sensor based on reduced graphene oxide (rGO)/Au/Co2CuS4 nanohybrid for determination of CAP in food and biological samples. The Co2CuS4 was synthesized from binuclear metal-organic framework (CoCu-BDC) through a two-step process. Nanohybrid was characterized by X-ray photoelectron spectroscopy and transmission electron microscopy. The rGO/Au/Co2CuS4 provides more active sites and good electrical conductivity to reduce charge transfer resistance and improve the electrocatalytic activity for determination of CAP. The prepared sensor has a wide linear range from 7 to 141 nM with a limit of detection of 2.5 nM and a limit of quantification of 21.92 nM. It also provided high selectivity and repeatability with a relative standard deviation of 2.6%. Stability studies showed that the electrode has acceptable performance with efficiency of 95% after 33 days.
Assuntos
Cloranfenicol , Cobalto , Técnicas Eletroquímicas , Ouro , Grafite , Grafite/química , Cloranfenicol/análise , Cloranfenicol/química , Técnicas Eletroquímicas/instrumentação , Ouro/química , Cobalto/química , Estruturas Metalorgânicas/química , Contaminação de Alimentos/análise , Limite de Detecção , Antibacterianos/análise , Antibacterianos/química , Nanopartículas/química , Animais , Óxidos/químicaRESUMO
The aim of this study was to evaluate the proportion of resistance to a temocillin, tigecycline, ciprofloxacin, and chloramphenicol phenotype called t2c2 that resulted from mutations within the ramAR locus among extended-spectrum ß-lactamases-Enterobacterales (ESBL-E) isolated in three intensive care units for 3 years in a French university hospital. Two parallel approaches were performed on all 443 ESBL-E included: (i) the minimal inhibitory concentrations of temocillin, tigecycline, ciprofloxacin, and chloramphenicol were determined and (ii) the genomes obtained from the Illumina sequencing platform were analyzed to determine multilocus sequence types, resistomes, and diversity of several tetR-associated genes including ramAR operon. Among the 443 ESBL-E strains included, isolates of Escherichia coli (n = 194), Klebsiella pneumoniae (n = 122), and Enterobacter cloacae complex (Ecc) (n = 127) were found. Thirty-one ESBL-E strains (7%), 16 K. pneumoniae (13.1%), and 15 Ecc (11.8%) presented the t2c2 phenotype in addition to their ESBL profile, whereas no E. coli presented these resistances. The t2c2 phenotype was invariably reversible by the addition of Phe-Arg-ß-naphthylamide, indicating a role of resistance-nodulation-division pumps in these observations. Mutations associated with the t2c2 phenotype were restricted to RamR, the ramAR intergenic region (IR), and AcrR. Mutations in RamR consisted of C- or N-terminal deletions and amino acid substitutions inside its DNA-binding domain or within key sites of protein-substrate interactions. The ramAR IR showed nucleotide substitutions involved in the RamR DNA-binding domain. This diversity of sequences suggested that RamR and the ramAR IR represent major genetic events for bacterial antimicrobial resistance.IMPORTANCEMorbimortality caused by infectious diseases is very high among patients hospitalized in intensive care units (ICUs). A part of these outcomes can be explained by antibiotic resistance, which delays the appropriate therapy. The transferable antibiotic resistance gene is a well-known mechanism to explain the high rate of multidrug resistance (MDR) bacteria in ICUs. This study describes the prevalence of chromosomal mutations, which led to additional antibiotic resistance among MDR bacteria. More than 12% of Klebsiella pneumoniae and Enterobacter cloacae complex strains presented mutations within the ramAR locus associated with a dysregulation of an efflux pump called AcrAB-TolC and a porin: OmpF. These dysregulations led to an increase in antibiotic output notably tigecycline, ciprofloxacin, and chloramphenicol associated with a decrease of input for beta-lactam, especially temocillin. Mutations within transcriptional regulators such as ramAR locus played a major role in antibiotic resistance dissemination and need to be further explored.
Assuntos
Antibacterianos , Proteínas de Bactérias , Farmacorresistência Bacteriana Múltipla , Klebsiella pneumoniae , beta-Lactamases , Humanos , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , beta-Lactamases/genética , beta-Lactamases/metabolismo , Cloranfenicol/farmacologia , Ciprofloxacina/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Enterobacter cloacae/genética , Enterobacter cloacae/efeitos dos fármacos , Enterobacter cloacae/enzimologia , Enterobacteriaceae/genética , Enterobacteriaceae/efeitos dos fármacos , Enterobacteriaceae/enzimologia , Infecções por Enterobacteriaceae/microbiologia , Escherichia coli/genética , Escherichia coli/efeitos dos fármacos , Unidades de Terapia Intensiva , Klebsiella pneumoniae/genética , Klebsiella pneumoniae/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Tipagem de Sequências Multilocus , Mutação , Tigeciclina/farmacologiaRESUMO
This study reports the development of a photocatalytic electrochemical aptasensor for the purpose of detecting chloramphenicol (CAP) antibiotic residues in water by utilizing SYBR Green I (SG) and chemically exfoliated MoS2 (ce-MoS2) as synergistically signal-amplification platforms. The Au nanoparticles (AuNPs) were electrodeposited onto the surface of an indium tin oxide (ITO) electrode. After that, the thiolate-modified cDNA, also known as capture DNA, was combined with the aptamer. Subsequently, photosensitized SG molecules and ce-MoS2 nanomaterial were inserted into the groove of the resultant double-stranded DNA (dsDNA). The activation of the photocatalytic process upon exposure to light resulted in the generation of singlet oxygen. The singlet oxygen effectively split the dsDNA, resulting in significant enhancement in the current of [Fe(CN)6]3-/4-. When the CAP was present, both SG molecules and ce-MoS2 broke away from the dsDNA, which turned off the photosensitization response, leading to significant reduction in the current of [Fe(CN)6]3-/4-. Under the optimal conditions, the aptasensor exhibited a linear relationship between the current of [Fe(CN)6]3-/4- with logarithmic concentrations of CAP from 20 to 1000 nM, with a detection of limit (3σ) of 3.391 nM. The aptasensor also demonstrated good selectivity towards CAP in the presence of interfering antibiotics, such as tetracycline, streptomycin, levofloxacin, ciprofloxacin, and sulfadimethoxine. Additionally, the results obtained from the analysis of natural water samples using the proposed aptasensor were consistent with the findings acquired through the use of a liquid chromatograph-mass spectrometer. Therefore, with its simplicity and high selectivity, this aptasensor can potentially detect alternative antibiotics in environmental water samples by replacing the aptamers based on photosensitization.
Assuntos
Aptâmeros de Nucleotídeos , Benzotiazóis , Técnicas Biossensoriais , Cloranfenicol , Diaminas , Dissulfetos , Técnicas Eletroquímicas , Molibdênio , Compostos Orgânicos , Quinolinas , Cloranfenicol/análise , Aptâmeros de Nucleotídeos/química , Técnicas Eletroquímicas/métodos , Molibdênio/química , Diaminas/química , Dissulfetos/química , Benzotiazóis/química , Quinolinas/química , Compostos Orgânicos/química , Técnicas Biossensoriais/métodos , Nanopartículas Metálicas/química , Ouro/química , Fármacos Fotossensibilizantes/química , Antibacterianos/análise , Limite de Detecção , Poluentes Químicos da Água/análise , Processos Fotoquímicos , Tamanho da PartículaRESUMO
A Ti3C2Tx/MoS2/MWCNT@rGONR nanocomposite was prepared for the first time for building a sensitive electrochemical aptasening platform to simultaneously detect kanamycin (Kana) and chloramphenicol (Cap). Owing to their accordion-like structure, rich surface groups, and high charge mobility, Ti3C2Tx/MoS2/MWCNT@rGONR composites provided a spacious covalent immobilization surface and a better electrochemical aptasensing platform. The aptamers of Kana and Cap used in sensors enhance the selectivity. Furthermore, TiP, an ion exchanger, was used for loading more different metal ions functioning as labels to form a sandwich-type sensor together with Ti3C2Tx/MoS2/MWCNT@rGONR, improving the electrochemical sensitivity and obtaining a highly distinguishable signal readout. Under the optimized conditions, the sensor has good detection limits of 0.135 nmol L-1 and 0.173 nmol L-1 for Kana and Cap, respectively, at the same linearity concentration of 0.5-2500 nmol L-1. Finally, it was successfully applied for detection in milk and fish meat, and the results were compared with the standard method HPLC, indicating its great potential for food safety monitoring.
Assuntos
Aptâmeros de Nucleotídeos , Técnicas Biossensoriais , Cloranfenicol , Técnicas Eletroquímicas , Contaminação de Alimentos , Canamicina , Leite , Titânio , Cloranfenicol/análise , Cloranfenicol/química , Canamicina/análise , Canamicina/química , Técnicas Eletroquímicas/métodos , Aptâmeros de Nucleotídeos/química , Titânio/química , Animais , Leite/química , Contaminação de Alimentos/análise , Técnicas Biossensoriais/métodos , Molibdênio/química , Limite de Detecção , Nanotubos de Carbono/química , Grafite/química , Nanocompostos/química , Análise de Alimentos/métodos , Antibacterianos/análise , Antibacterianos/química , Peixes , DissulfetosRESUMO
Chloramphenicol (CAP) poses a threat to human health due to its toxicity and bioaccumulation, and it is very important to measure it accurately and sensitively. This work explored a host-guest recognition strategy to mediate dual aggregation-induced electrochemiluminescence (AIECL) of 1,1,2,2-tetrakis(4-(pyridin-4-yl) phenyl)-ethene (TPPE) for ratio detection of CAP, in which, cucurbit[8]uril (CB[8]) served as host to assemble guest TPPE. The resulting supramolecular complex CB[8]-TPPE exhibited excellent dual-AIECL-emission with signal strength approximately four times that of TPPE aggregates and black hole quencher-1 (BHQ1) could efficiently quench dual-AIECL signal. CB[8]-TPPE coupled dual-function quencher BHQ1 and high-efficiency DNA reactor to achieve ultra-sensitive detection of CAP, exhibiting a linearity range of 10 fmol·L-1-100 nmol·L-1 and limit of detection of 1.81 fmol·L-1. CB[8]-TPPE provides a novel way to improve the dual-emission of TPE derivatives and sets up a promising platform for CAP detection, demonstrating a good practical application potential.
Assuntos
Cloranfenicol , Técnicas Eletroquímicas , Medições Luminescentes , Cloranfenicol/análise , Cloranfenicol/química , Técnicas Eletroquímicas/instrumentação , Medições Luminescentes/instrumentação , Medições Luminescentes/métodos , Limite de Detecção , Antibacterianos/química , Antibacterianos/análise , Contaminação de Alimentos/análise , Hidrocarbonetos Aromáticos com Pontes/química , Luminescência , Imidazóis/química , Compostos Heterocíclicos com 2 Anéis , Compostos Macrocíclicos , ImidazolidinasRESUMO
Toxic organic and heavy metal contaminants commonly exist in industrial waste stream(s) and treatment is of great challenge. In this study, a dielectric barrier discharge (DBD) non-thermal plasma technology was employed for the simultaneous treatment of two important contaminants, chloramphenicol (CAP) and Cr(VI) in an aqueous solution through redox transformations. More than 70% of CAP and 20% of TOC were degraded in 60 min, while Cr(VI) was completely removed in 10 min. The hydroxyl radicals were the main active species for the degradation. Meanwhile, the consumption of hydroxyl radicals was beneficial to the reduction of Cr(VI). The synergistic effect was investigated between CAP degradation and Cr(VI) reduction. The reduction of Cr(VI) would be enhanced in the presence of CAP with a low concentration and could be inhibited under a high concentration, because part of hydroxyl radicals could be consumed by the low-concentration CAP and the obtained intermediates with a higher kinetic rate. However, CAP with a high concentration could react with such reductive species as eaq- and â¢H, which could compete with Cr(VI) and inhibit the reduction. In addition, the presence of Cr(VI) enhanced the degradation and mineralization of CAP; the study of obtained intermediates indicated that the presence of Cr(VI) changed the degradation path of CAP as Cr(VI) would react with reductive species, enhance the generation of hydroxyl radicals, and cause more hydroxylation reactions. Moreover, the mechanism for the simultaneous redox transformations of CAP and Cr(VI) was illustrated. This study indicates that the DBD non-thermal plasma technology can be one of better solutions for simultaneous elimination of heavy metal and organic contaminants in aquatic environments.
Assuntos
Cloranfenicol , Cromo , Oxirredução , Poluentes Químicos da Água , Cloranfenicol/química , Cromo/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/análise , Gases em Plasma/química , Radical Hidroxila/química , Eliminação de Resíduos Líquidos/métodosRESUMO
This study aimed to investigate the potential mechanisms associated with the persistence of chloramphenicol (CHP) resistance in Escherichia coli and Salmonella enterica isolated from pigs, pork, and humans in Thailand. The CHP-resistant E. coli (n = 106) and Salmonella (n = 57) isolates were tested for their CHP susceptibility in the presence and absence of phenylalanine arginine ß-naphthylamide (PAßN). The potential co-selection of CHP resistance was investigated through conjugation experiments. Whole genome sequencing (WGS) was performed to analyze the E. coli (E329, E333, and E290) and Salmonella (SA448, SA461, and SA515) isolates with high CHP MIC (32-256 µg/mL) and predominant plasmid replicon types. The presence of PAßN significantly reduced the CHP MICs (≥4-fold) in most E. coli (67.9%) and Salmonella (64.9%). Ampicillin, tetracycline, and streptomycin co-selected for CHP-resistant Salmonella and E. coli-transconjugants carrying cmlA. IncF plasmids were mostly detected in cmlA carrying Salmonella (IncFIIAs) and E. coli (IncFIB and IncF) transconjugants. The WGS analysis revealed that class1 integrons with cmlA1 gene cassette flanked by IS26 and TnAs1 were located on IncX1 plasmid, IncFIA(HI1)/HI1B plasmids and IncFII/FIB plasmids. IncFIA(HI1)/HI1B/Q1in SA448 contained catA flanked by IS1B and TnAs3. In conclusion, cross resistance through proton motive force-dependent mechanisms and co-selection by other antimicrobial agents involved the persistence of CHP-resistance in E. coli in this collection. Dissemination of CHP-resistance genes was potentially facilitated by mobilization via mobile genetic elements.
Assuntos
Escherichia coli , Testes de Sensibilidade Microbiana , Plasmídeos , Animais , Escherichia coli/genética , Escherichia coli/efeitos dos fármacos , Tailândia , Suínos , Humanos , Plasmídeos/genética , Salmonella/genética , Salmonella/efeitos dos fármacos , Antibacterianos/farmacologia , Resistência ao Cloranfenicol/genética , Cloranfenicol/farmacologia , Sequenciamento Completo do GenomaRESUMO
Two levels of nucleic acids-based isothermal amplification normally require a long reaction time due to the low concentration of catalyst, which limits its practical application. A sensitive fluorescence assay of chloramphenicol (CAP) was developed coupled with two-level isothermal amplification using a self-powered catalyzed hairpin assembly (CHA) and entropy-driven circuit (EDC). CAP can bind with its aptamer to open its closed structure. The opened hairpin can initiate self-powered CHA and EDC. The product of CHA can circularly catalyze the CHA with increasing concentration. In principle, the product of CHA plays the role of catalyst and increases with the progression of the reaction. Compared with the normal two levels of amplification, the amplification efficiency of our strategy is much higher due to the self-powered reaction by the CHA product. Thus, the reaction time is shortened to 110 min in this strategy. Moreover, the detection limit for CAP can achieve 0.1 pM and shows promising prospects for practical application.
Assuntos
Cloranfenicol , Entropia , Limite de Detecção , Técnicas de Amplificação de Ácido Nucleico , Cloranfenicol/análise , Cloranfenicol/química , Técnicas de Amplificação de Ácido Nucleico/métodos , Catálise , Espectrometria de Fluorescência/métodos , Fluorescência , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Técnicas de Diagnóstico MolecularRESUMO
A middle-aged male patient presented with a central corneal perforation in a deep stromal infiltrate in his left eye. An emergency therapeutic penetrating keratoplasty was performed. Microbiological evaluation of the corneal scraping specimen revealed septate fungal filaments on stains. However, culture reports after 24 hours from the scraping sample and the excised half corneal button showed growth of gram-negative bacilli. This pathogen was identified as an aerobic, non-fermentative, gram-negative, bacillus by conventional microbiology and confirmed as Myroides species by the VITEK 2 Compact system (bioMérieux, Marcy l'Etoile, France). Susceptibility to chloramphenicol was noted based on which the patient was treated with topical chloramphenicol 0.5%. No recurrence of the infection was noted. This is the first reported case of corneal infection with the Myroides species of bacteria which, heretofore, have been known to cause endocarditis and urinary tract infections.
Assuntos
Infecções Oculares Fúngicas , Ceratite , Humanos , Masculino , Pessoa de Meia-Idade , Ceratite/microbiologia , Ceratite/diagnóstico , Ceratite/tratamento farmacológico , Infecções Oculares Fúngicas/microbiologia , Infecções Oculares Fúngicas/diagnóstico , Infecções Oculares Fúngicas/tratamento farmacológico , Antibacterianos/uso terapêutico , Ceratoplastia Penetrante , Cloranfenicol/uso terapêutico , Cloranfenicol/administração & dosagem , Infecções Oculares Bacterianas/microbiologia , Infecções Oculares Bacterianas/diagnóstico , Infecções Oculares Bacterianas/tratamento farmacológico , Perfuração da Córnea/microbiologia , Perfuração da Córnea/diagnósticoRESUMO
AIMS: Enterococcus faecalis (E. faecalis) is a leading cause of nosocomial infection and presents a wide spectrum of antibiotic resistance, being vancomycin-resistant Enterococcus (VRE) one of the most relevant. Synthetic antimicrobial peptides (SAMPs) are currently a promising option to overcome antimicrobial resistance. Thus, the purpose of this study was to assess the effect of eight SAMPs against vancomycin-resistant E. faecalis, as well as to investigate their mechanism of action and synergy with conventional antibiotics. METHODS AND RESULTS: Here, eight SAMPs, Mo-CBP3-PepI, Mo-CBP3-PepII, Mo-CBP3-PepIII, RcAlb-PepI, RcAlb-PepII, RcAlb-PepIII, PepGAT, and PepKAA, were tested for antibacterial activity in vitro against E. faecalis (ATCC® 51299) through broth microdilution. A maximum of 48% of E. faecalis growth inhibition was achieved by treatment with SAMPs alone. However, when these peptides were combined with the antibiotic chloramphenicol, assessed by checkerboard method, the inhibition increased to 55%-76% of inhibition, two to three-folds of increase if compared to the effects of the compounds alone. Microscopic analysis showed that E. faecalis cells treated with a combination of SAMPs and chloramphenicol resulted in bacterial membrane damage. The biofilm inhibition maximum was 22% for SAMPs alone, when combined with chloramphenicol, the maximum increased to 33%. CONCLUSIONS: SAMPs and their combination with chloramphenicol demonstrate antibacterial activity against E. faecalis, possibly by inducing bacterial membrane damage.
Assuntos
Antibacterianos , Peptídeos Antimicrobianos , Cloranfenicol , Sinergismo Farmacológico , Enterococcus faecalis , Testes de Sensibilidade Microbiana , Enterococos Resistentes à Vancomicina , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/crescimento & desenvolvimento , Antibacterianos/farmacologia , Cloranfenicol/farmacologia , Peptídeos Antimicrobianos/farmacologia , Enterococos Resistentes à Vancomicina/efeitos dos fármacos , Vancomicina/farmacologiaRESUMO
Inspired by the desert beetle, a novel biomimetic chip was developed to detect chloramphenicol (CP). The chip was characterized by a periodic array in which hydrophobic Au nanoparticles (AuNPs) were semi-embedded on hydrophilic polymethyl methacrylate (PMMA) spheres. Among them, the AuNPs exhibited both a localized surface plasmon resonance effect to amplify the reflected signal and a synergistic effect with PMMA spheres to create a significant hydrophilic-hydrophobic interface, which facilitated the enrichment of target CP molecules and improved sensitivity. After optimization, the chip showed direct, ultrasensitive (as low as 0.2 ng/mL), fast (5 min), and selective detection of CP with a wide concentration range extending from 0.2 ng/mL to 1000 ng/mL. During detection, color changes of the chip were observed by naked eyes without any color display equipment. The recovery of CP was between 94.65 % and 108.70 % in chicken and milk samples.
Assuntos
Galinhas , Cloranfenicol , Besouros , Contaminação de Alimentos , Ouro , Nanopartículas Metálicas , Leite , Cloranfenicol/análise , Cloranfenicol/química , Animais , Ouro/química , Nanopartículas Metálicas/química , Besouros/química , Contaminação de Alimentos/análise , Leite/química , Antibacterianos/análise , Antibacterianos/química , Coloides/químicaRESUMO
Misuse of chloramphenicol (CAP) can lead to severe food safety issues. Therefore, the accurate and sensitive detection of CAP residues is important for public health. Herein, a convenient and reliable interfacial self-assembly technique was used to form a uniform Au@Ag nanobipyramids (NBPs) film on an ordered SiO2 nanosphere array (SiO2 NS), which served as a Raman-enhanced substrate. In conjunction with a deoxyribonucleic acid enzyme-induced signal amplification strategy, we developed a novel surface-enhanced Raman scattering (SERS) biosensor for the selective and sensitive detection of CAP. The biosensor exhibited a detection limit of 6.42 × 10-13 mol·L-1 and a detection range of 1.0 × 10-12-1.0 × 10-6 mol·L-1. The biosensor could detect CAP in spiked milk samples with a high accuracy, and its recovery rates ranged from 97.88% to 107.86%. The as-developed biosensor with the advantages of high sensitivity and high selectivity offers a new strategy for the rapid, reliable and sensitive detection of CAP, rendering it applicable to food safety control.
Assuntos
Técnicas Biossensoriais , Cloranfenicol , DNA Catalítico , Contaminação de Alimentos , Ouro , Limite de Detecção , Leite , Dióxido de Silício , Prata , Análise Espectral Raman , Dióxido de Silício/química , Cloranfenicol/análise , Ouro/química , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Prata/química , Análise Espectral Raman/métodos , Análise Espectral Raman/instrumentação , Contaminação de Alimentos/análise , Leite/química , DNA Catalítico/química , Animais , Nanopartículas Metálicas/química , Antibacterianos/análiseRESUMO
Zero-valent iron (ZVI) has been extensively studied for its capacity to remove various contaminants in the environments. However, whether ZVI affects bacterial resistance to antibiotics has not been fully explored. Herein, it was unexpected that, compared with microscale ZVI (mZVI), nanoscale ZVI (nZVI) facilitated the susceptibility of Pseudomonas aeruginosa (P. aeruginosa) to chloramphenicol (CAP), with a decrease in the minimal inhibitory concentration (MIC) of about 60 %, demonstrating a nanosize-specific effect. nZVI enhanced CAP accumulation in P. aeruginosa via inhibitory effect on efflux pumps activated by MexT, thus conferring the susceptibility of P. aeruginosa to CAP. Circular dichroism spectroscopy revealed that the structure of MexT was changed during the evolution. More importantly, molecular dynamic simulations uncovered that, once the structure of MexT changed, it would be more likely to interact with nZVI, resulting in more serious changes in its secondary structure, which was consistent with the increasing susceptibility of P. aeruginosa to CAP. Collectively, this study elucidated the size-specific effect and the underlying mechanism of ZVI on the bacterial evolution of susceptibility toward antibiotics, highlighting the potentials of nZVI-based technologies on the prevention of bacterial resistance to antibiotics, one of the most important issue for globally public health.
Assuntos
Antibacterianos , Cloranfenicol , Farmacorresistência Bacteriana , Ferro , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa , Pseudomonas aeruginosa/efeitos dos fármacos , Cloranfenicol/farmacologia , Cloranfenicol/química , Antibacterianos/farmacologia , Antibacterianos/química , Ferro/química , Farmacorresistência Bacteriana/efeitos dos fármacos , Nanopartículas Metálicas/química , Simulação de Dinâmica Molecular , Proteínas de Bactérias/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/genéticaRESUMO
BACKGROUND: Simultaneous detection of food contaminants is crucial in addressing the collective health hazards arising from the presence of multiple contaminants. However, traditional multi-competitive surface-enhanced Raman scattering (SERS) aptasensors face difficulties in achieving simultaneous accurate detection of multiple target substances due to the uncontrollable SERS "hot spots". In this study, using chloramphenicol (CAP) and estradiol (E2) as two target substances, we introduced a novel approach that combines machine learning methods with a dual SERS aptasensor, enabling simultaneous high-sensitivity and accurate detection of both target substances. RESULTS: The strategy effectively minimizes the interference from characteristic Raman peaks commonly encountered in traditional multi-competitive SERS aptasensors. For this sensing system, the Au@4-MBA@Ag nanoparticles modified with sulfhydryl (SH)-CAP aptamer and Au@DTNB@Ag NPs modified with sulfhydryl (SH)-E2 aptamer were used as signal probes. Additionally, Fe3O4@Au nanoflowers integrated with SH-CAP aptamer complementary DNA and SH-E2 aptamer complementary DNA were used as capture probes, respectively. When compared to linear regression random forest, and support vector regression (SVR) models, the proposed artificial neural network (ANN) model exhibited superior precision, demonstrating R2 values of 0.963, 0.976, 0.991, and 0.970 for the training set, test set, validation set, and entire dataset, respectively. Validation with ten spectral groups reported an average error of 244 µg L-1. SIGNIFICANCE: The essence of our study lies in its capacity to address a persistent challenge encountered by traditional multiple competitive SERS aptasensors - the interference generated by uncontrollable SERS "hot spots" that hinders simultaneous quantification. The accuracy of the predictive model for simultaneous detection of two target substances was significantly improved using machine learning tools. This innovative technique offers promising avenues for the accurate and high-sensitive simultaneous detection of multiple food and environmental contaminants.
Assuntos
Aptâmeros de Nucleotídeos , Ouro , Aprendizado de Máquina , Nanopartículas Metálicas , Prata , Análise Espectral Raman , Aptâmeros de Nucleotídeos/química , Prata/química , Ouro/química , Nanopartículas Metálicas/química , Cloranfenicol/análise , Estradiol/análise , Técnicas Biossensoriais/métodos , Contaminação de Alimentos/análise , Limite de DetecçãoRESUMO
Many regulatory genes that affect cellular development in Streptomyces, such as the canonical bld genes, have already been identified. However, in this study, we identified sven_5003 in Streptomyces venezuelae as a major new developmental regulatory gene, the deletion of which leads to a bald phenotype, typical of bld mutants, under multiple growth conditions. Our data indicated that disruption of sven_5003 also has a differential impact on the production of the two antibiotics jadomycin and chloramphenicol. Enhanced production of jadomycin but reduced production of chloramphenicol were detected in our sven_5003 mutant strain (S. venezuelae D5003). RNA-Seq analysis indicated that SVEN_5003 impacts expression of hundreds of genes, including genes involved in development, primary and secondary metabolism, and genes of unknown function, a finding confirmed by real-time PCR analysis. Transcriptional analysis indicated that sven_5003 is an auto-regulatory gene, repressing its own expression. Despite the evidence indicating that SVEN_5003 is a regulatory factor, a putative DNA-binding domain was not predicted from its primary amino acid sequence, implying an unknown regulatory mechanism by SVEN_5003. Our findings revealed that SVEN_5003 is a pleiotropic regulator with a critical role in morphological development in S. venezuelae.