RESUMO
A relaxin-like gonad-stimulating peptide (RGP) in starfish was the first identified invertebrate gonadotropin responsible for final gamete maturation. An RGP ortholog was newly identified from Astropecten scoparius of the order Paxillosida. The A. scoparius RGP (AscRGP) precursor is encoded by a 354 base pair open reading frame and is a 118 amino acid (aa) protein consisting of a signal peptide (26 aa), B-chain (21 aa), C-peptide (47 aa), and A-chain (24 aa). There are three putative processing sites (Lys-Arg) between the B-chain and C-peptide, between the C-peptide and A-chain, and within the C-peptide. This structural organization revealed that the mature AscRGP is composed of A- and B-chains with two interchain disulfide bonds and one intrachain disulfide bond. The C-terminal residues of the B-chain are Gln-Gly-Arg, which is a potential substrate for formation of an amidated C-terminal Gln residue. Non-amidated (AscRGP-GR) and amidated (AscRGP-NH2 ) peptides were chemically synthesized and their effect on gamete shedding activity was examined using A. scoparius ovaries. Both AscRGP-GR and AscRGP-NH2 induced oocyte maturation and ovulation in similar dose-dependent manners. This is the first report on a C-terminally amidated functional RGP. Collectively, these results suggest that AscRGP-GR and AscRGP-NH2 act as a natural gonadotropic hormone in A. scoparius.
Assuntos
Gonadotropinas/química , Gonadotropinas/metabolismo , Hormônios de Invertebrado/química , Hormônios de Invertebrado/metabolismo , Neuropeptídeos/química , Neuropeptídeos/metabolismo , Oócitos/metabolismo , Ovário/metabolismo , Estrelas-do-Mar/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Células Cultivadas , Feminino , Gonadotropinas/síntese química , Gonadotropinas/farmacologia , Hormônios de Invertebrado/síntese química , Hormônios de Invertebrado/farmacologia , Neuropeptídeos/síntese química , Neuropeptídeos/farmacologia , Oócitos/efeitos dos fármacos , Oogênese/efeitos dos fármacos , Ovário/efeitos dos fármacos , Ovulação/efeitos dos fármacos , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Nervo Radial/metabolismo , Estrelas-do-Mar/efeitos dos fármacos , Estrelas-do-Mar/genéticaRESUMO
Molluscan pedal peptides (PPs) and arthropod orcokinins (OKs) are prototypes of a family of neuropeptides that have been identified in several phyla. Recently, starfish myorelaxant peptide (SMP) was identified as a PP/OK-type neuropeptide in the starfish Patiria pectinifera (phylum Echinodermata). Furthermore, analysis of transcriptome sequence data from the starfish Asterias rubens revealed two PP/OK-type precursors: an SMP-type precursor (A. rubens PP-like neuropeptide precursor 1; ArPPLNP1) and a second precursor (ArPPLNP2). We reported previously a detailed analysis of ArPPLNP1 expression in A. rubens and here we report the first functional characterization ArPPLNP2-derived neuropeptides. Sequencing of a cDNA encoding ArPPLNP2 revealed that it comprises eleven related neuropeptides (ArPPLN2a-k), the structures of several of which were confirmed using mass spectrometry. Analysis of the expression of ArPPLNP2 and neuropeptides derived from this precursor using mRNA in situ hybridization and immunohistochemistry revealed a widespread distribution, including expression in radial nerve cords, circumoral nerve ring, digestive system, tube feet and innervation of interossicular muscles. In vitro pharmacology revealed that the ArPPLNP2-derived neuropeptide ArPPLN2h has no effect on the contractility of tube feet or the body wall-associated apical muscle, contrasting with the relaxing effect of ArPPLN1b (ArSMP) on these preparations. ArPPLN2h does, however, cause dose-dependent relaxation of cardiac stomach preparations, with greater potency/efficacy than ArPPLN1b and with similar potency/efficacy to the SALMFamide neuropeptide S2. In conclusion, there are similarities in the expression patterns of ArPPLNP1 and ArPPLNP2 but our data also indicate specialization in the roles of neuropeptides derived from these two PP/OK-type precursors in starfish.
Assuntos
Asterias/anatomia & histologia , Asterias/metabolismo , Neuropeptídeos/metabolismo , Transdução de Sinais/fisiologia , Sequência de Aminoácidos , Animais , Clonagem Molecular , Espectrometria de Massas , Neuropeptídeos/genética , Neuropeptídeos/farmacologia , RNA Mensageiro/metabolismo , Nervo Radial/efeitos dos fármacos , Nervo Radial/metabolismo , Transdução de Sinais/efeitos dos fármacos , TranscriptomaRESUMO
The general view is that both glycine (Eccles, 1964) and GABA (Curtis and Felix, 1971) evoke postsynaptic inhibition in spinal motor neurons. In newborn or juvenile animals, there are conflicting results showing postsynaptic inhibition in motor neurons by corelease of GABA and glycine (Jonas et al., 1998) or by glycine alone (Bhumbra et al., 2012). To resolve the relative contributions of GABA and glycine to postsynaptic inhibition, we performed in vivo intracellular recordings from forelimb motor neurons in adult mice. Postsynaptic potentials evoked from segmental, propriospinal, and bulbospinal systems in motor neurons were compared across four different conditions: control, after gabazine, gabazine followed by strychnine, and strychnine alone. No significant differences were observed in the proportion of IPSPs and EPSPs between control and gabazine conditions. In contrast, EPSPs but not IPSPs were recorded after adding strychnine with gabazine or administering strychnine alone, suggesting an exclusive role for glycine in postsynaptic inhibition. To test whether the injected (intraperitoneal) dose of gabazine blocked GABAergic inhibitory transmission, we evoked GABAA receptor-mediated monosynaptic IPSPs in deep cerebellar nuclei neurons by stimulation of Purkinje cell fibers. No monosynaptic IPSPs could be recorded in the presence of gabazine, showing the efficacy of gabazine treatment. Our results demonstrate that, in the intact adult mouse, the postsynaptic inhibitory effects in spinal motor neurons exerted by three different systems, intrasegmental and intersegmental as well as supraspinal, are exclusively glycinergic. These findings emphasize the importance of glycinergic postsynaptic inhibition in motor neurons and challenge the view that GABA also contributes.
Assuntos
Glicina/metabolismo , Potenciais Pós-Sinápticos Inibidores , Neurônios Motores/metabolismo , Nervo Radial/fisiologia , Ácido gama-Aminobutírico/metabolismo , Animais , Antagonistas GABAérgicos/farmacologia , Glicinérgicos/farmacologia , Camundongos , Neurônios Motores/efeitos dos fármacos , Neurônios Motores/fisiologia , Células de Purkinje/fisiologia , Piridazinas/farmacologia , Nervo Radial/citologia , Nervo Radial/metabolismo , Estricnina/farmacologiaRESUMO
The cerebral cortex performs complex cognitive functions at the expense of tremendous energy consumption. Blood vessels in the brain are known to form stereotypic patterns that facilitate efficient oxygen and nutrient delivery. Yet little is known about how vessel development in the brain is normally regulated. Radial glial neural progenitors are well known for their central role in orchestrating brain neurogenesis. Here we show that, in the late embryonic cortex, radial glial neural progenitors also play a key role in brain angiogenesis, by interacting with nascent blood vessels and regulating vessel stabilization via modulation of canonical Wnt signaling. We find that ablation of radial glia results in vessel regression, concomitant with ectopic activation of Wnt signaling in endothelial cells. Direct activation of Wnt signaling also results in similar vessel regression, while attenuation of Wnt signaling substantially suppresses regression. Radial glial ablation and ectopic Wnt pathway activation leads to elevated endothelial expression of matrix metalloproteinases, while inhibition of metalloproteinase activity significantly suppresses vessel regression. These results thus reveal a previously unrecognized role of radial glial progenitors in stabilizing nascent brain vascular network and provide novel insights into the molecular cascades through which target neural tissues regulate vessel stabilization and patterning during development and throughout life.
Assuntos
Encéfalo/irrigação sanguínea , Córtex Cerebral/irrigação sanguínea , Células-Tronco Neurais/metabolismo , Complexo de Reconhecimento de Origem/genética , Nervo Radial/embriologia , Animais , Pontos de Checagem do Ciclo Celular , Células Cultivadas , Proteínas do Olho/genética , Feminino , Proteínas de Homeodomínio/genética , Masculino , Metaloproteinase 2 da Matriz/biossíntese , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/biossíntese , Metaloproteinase 9 da Matriz/metabolismo , Camundongos , Camundongos Transgênicos , Neovascularização Fisiológica , Neurogênese , Neuroglia/metabolismo , Fator de Transcrição PAX6 , Fatores de Transcrição Box Pareados/genética , RNA Mensageiro/biossíntese , Nervo Radial/metabolismo , Proteínas Repressoras/genética , Proteínas Wnt/genética , Proteínas Wnt/metabolismo , Via de Sinalização WntRESUMO
BACKGROUND: Since the 18th century, the existence of ulnar nerve innervation of the medial head of the triceps brachii muscle has been controversial. The evidence for or against such innervation has been based on macroscopic dissection, an unsuitable method for studying intraneural topography or intramuscular branching. The study of smaller specimens (embryos or fetuses) by means of serial histologic sections may resolve the controversy. QUESTIONS/PURPOSES: Using fetal specimens and histology we determined the contributions of the ulnar and radial nerves to innervation of the triceps brachii muscle. METHODS: We histologically examined 15 embryonic and fetal arms. Radial nerve branches obtained from six adult arms were analyzed immunohistochemically to determine motor fiber content. RESULTS: The medial head of the triceps brachii muscle was always innervated by the radial nerve (ulnar collateral branch). The branches seeming to leave the ulnar nerve at elbow level were the continuation of the radial nerve that had joined the ulnar nerve sheath via a connection in the axillary region. Immunohistochemistry revealed motor and nonmotor fibers in this radial nerve branch. CONCLUSIONS: A connection between the radial and ulnar nerves sometimes may exist, resulting in an apparent ulnar nerve origin of muscular branches to the medial head of the triceps, even though in all our specimens the fibers could be traced back to the radial nerve. CLINICAL RELEVANCE: Before performing or suggesting new muscle and nerve transpositions using this apparent ulnar innervation, the real origin should be confirmed to avoid failure.
Assuntos
Braço/anatomia & histologia , Embrião de Mamíferos/embriologia , Feto/embriologia , Músculo Esquelético/inervação , Nervo Ulnar/embriologia , Biomarcadores/metabolismo , Cadáver , Feminino , Idade Gestacional , Humanos , Masculino , Músculo Esquelético/embriologia , Nervo Radial/anatomia & histologia , Nervo Radial/metabolismoRESUMO
Echinoderms, as invertebrate deuterostomes, have amazing neuronal intrinsic growth aptitude triggered at any time point during the animal lifespan leading to successful functional tissue regrowth. This trait is known to be in opposition to their mammal close phylogenic relatives that have lost the ability to regenerate their central nervous system. Despite the promising nature of this intrinsic echinoderm trait, it was only recently that this complex biological event started to be unveiled. In the present study, a 2DE gel-based phosphoproteomics approach was used to investigate changes in starfish neuronal protein phosphorylation states at two different wound healing time-graded events following arm tip amputation, 48 h and 13 days. Among the resolved protein spots in 3.0-5.6 NL pH IEF strips, 190, 142, and 124 had a phosphoprotein signal in the control and the two injury experimental groups, respectively. Gel image analysis, highlighted 129 spots with an injury-related protein phosphorylation dynamics, several being exclusively phosphorylated in controls (72 spots), injured nerves (8 spots) or, showing significantly different phosphorylation ratios (37 spots). Within these, a total of 43 proteins were identified with MALDI-TOF/TOF. Altogether, several intervening proteins of important injury-signaling pathways that seem to be modulated through phosphorylation, were identified for the first time in starfish radial nerve cord early regeneration events. These include cytoskeleton re-organization toward the formation of the neuronal growth cones; cell membrane rearrangements, actin filaments, and microtubules dynamics; mRNA binding and transport; lipid signaling; Notch pathway; and neuropeptide processing.
Assuntos
Regeneração Nervosa/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Fosfoproteínas/metabolismo , Nervo Radial/fisiologia , Estrelas-do-Mar/fisiologia , Cicatrização/fisiologia , Animais , Eletroforese em Gel Bidimensional , Proteínas do Tecido Nervoso/análise , Proteínas do Tecido Nervoso/química , Fosfoproteínas/análise , Fosfoproteínas/química , Fosforilação , Proteoma/análise , Proteoma/química , Proteômica/métodos , Nervo Radial/metabolismo , Estrelas-do-Mar/metabolismoRESUMO
The life history of sea urchins is fundamentally different from that of traditional models of aging and therefore they provide the opportunity to gain new insight into this complex process. Sea urchins grow indeterminately, reproduce throughout their life span and some species exhibit negligible senescence. Using a microarray and qRT-PCR, age-related changes in gene expression were examined in three tissues (muscle, esophagus and nerve) of the sea urchin species Strongylocentrotus purpuratus. The results indicate age-related changes in gene expression involving many key cellular functions such as the ubiquitin-proteasome pathway, DNA metabolism, signaling pathways and apoptosis. Although there are tissue-specific differences in the gene expression profiles, there are some characteristics that are shared between tissues providing insight into potential mechanisms that promote lack of senescence in these animals. As an example, there is an increase in expression of genes encoding components of the Notch signaling pathway with age in all three tissues and a decrease in expression of the Wnt1 gene in both muscle and nerve. The interplay between the Notch and Wnt pathways may be one mechanism that ensures continued regeneration of tissues with advancing age contributing to the general lack of age-related decline in these animals.
Assuntos
Envelhecimento/genética , Regulação da Expressão Gênica , Strongylocentrotus purpuratus/crescimento & desenvolvimento , Animais , Esôfago/metabolismo , Músculo Esquelético/metabolismo , Complexo de Endopeptidases do Proteassoma/metabolismo , Nervo Radial/metabolismo , Receptores Notch/metabolismo , Strongylocentrotus purpuratus/metabolismo , Ubiquitina/metabolismo , Via de Sinalização Wnt/fisiologiaRESUMO
We report here a unique amyloidoma of the radial nerve which could not be subtyped by available techniques, including immunohistochemistry and standard clinical and laboratory evaluation. In order to identify the amyloid monomer, we developed a novel preparative procedure designed to optimize conditions for liquid chromatography tandem mass spectrometry analysis of formalin-fixed/paraffin-embedded (FFPE) tissue. Subsequent mass spectrometric analysis clearly identified kappa light chain as the monomer, with no evidence of lambda light chain. Manual interpretation of the matched spectra revealed no evidence of polyclonality. This study also enabled detailed characterisation of twelve likely amyloid matrix components. Finally, our analysis revealed extensive hydroxylation of collagen type I but, unexpectedly, an almost complete lack of hydroxylated residues in the normally heavily-hydroxylated collagen type VI chains, pointing to structural/functional alterations of collagen VI in this matrix that could have contributed to the pathogenesis of this very unusual tumour. Given the high quality of the data here acquired using a standard quadrupole-time of flight tandem mass spectrometer of modest performance, the robust and straightforward preparative method described constitutes a competitive alternative to more involved approaches using state-of-the-art equipment.
Assuntos
Amiloidose/patologia , Placa Amiloide/patologia , Nervo Radial/patologia , Espectrometria de Massas em Tandem/métodos , Sequência de Aminoácidos , Amiloidose/metabolismo , Cromatografia Líquida de Alta Pressão , Colágeno Tipo VI/química , Colágeno Tipo VI/metabolismo , Feminino , Fixadores , Secções Congeladas , Humanos , Hidroxilação , Cadeias kappa de Imunoglobulina/química , Cadeias kappa de Imunoglobulina/metabolismo , Pessoa de Meia-Idade , Dados de Sequência Molecular , Inclusão em Parafina , Placa Amiloide/metabolismo , Nervo Radial/metabolismo , Fixação de TecidosRESUMO
Radial sorting allows the segregation of axons by a single Schwann cell (SC) and is a prerequisite for myelination during peripheral nerve development. Radial sorting is impaired in models of human diseases, congenital muscular dystrophy (MDC) 1A, MDC1D and Fukuyama, owing to loss-of-function mutations in the genes coding for laminin α2, Large or fukutin glycosyltransferases, respectively. It is not clear which receptor(s) are activated by laminin 211, or glycosylated by Large and fukutin during sorting. Candidates are αß1 integrins, because their absence phenocopies laminin and glycosyltransferase deficiency, but the topography of the phenotypes is different and ß1 integrins are not substrates for Large and fukutin. By contrast, deletion of the Large and fukutin substrate dystroglycan does not result in radial sorting defects. Here, we show that absence of dystroglycan in a specific genetic background causes sorting defects with topography identical to that of laminin 211 mutants, and recapitulating the MDC1A, MDC1D and Fukuyama phenotypes. By epistasis studies in mice lacking one or both receptors in SCs, we show that only absence of ß1 integrins impairs proliferation and survival, and arrests radial sorting at early stages, that ß1 integrins and dystroglycan activate different pathways, and that the absence of both molecules is synergistic. Thus, the function of dystroglycan and ß1 integrins is not redundant, but is sequential. These data identify dystroglycan as a functional laminin 211 receptor during axonal sorting and the key substrate relevant to the pathogenesis of glycosyltransferase congenital muscular dystrophies.
Assuntos
Axônios/fisiologia , Movimento Celular/genética , Distroglicanas/fisiologia , Integrina beta1/fisiologia , Nervo Radial/fisiologia , Animais , Axônios/efeitos dos fármacos , Axônios/metabolismo , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Distroglicanas/genética , Distroglicanas/metabolismo , Humanos , Integrina beta1/genética , Integrina beta1/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Distrofias Musculares/genética , Distrofias Musculares/metabolismo , Bainha de Mielina/metabolismo , RNA Interferente Pequeno/farmacologia , Nervo Radial/efeitos dos fármacos , Nervo Radial/metabolismo , Ratos , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Transdução de Sinais/fisiologia , Fatores de TempoRESUMO
We describe the first proteomic characterization of the radial nerve cord (RNC) of an echinoderm, the sea star Marthasterias glacialis. The combination of 2-DE with MS (MALDI-TOF/TOF) resulted in the identification of 286 proteins in the RNC. Additionally, 158 proteins were identified in the synaptosomal membranes enriched fraction after 1-DE separation. The 2-DE RNC reference map is available via the WORLD-2DPAGE Portal (http://www.expasy.ch/world-2dpage/) along with the associated protein identification data which are also available in the PRIDE database. The identified proteins constitute the first high-throughput evidence that seems to indicate that echinoderms nervous transmission relies primarily on chemical synapses which is similar to the synaptic activity in adult mammal's spinal cord. Furthermore, several homologous proteins known to participate in the regeneration events of other organisms were also identified, and thus can be used as targets for future studies aiming to understand the poorly uncharacterized regeneration capability of echinoderms. This "echinoderm missing link" is also a contribution to unravel the mystery of deuterostomian CNS evolution.
Assuntos
Proteínas/metabolismo , Proteoma/metabolismo , Nervo Radial/metabolismo , Estrelas-do-Mar/metabolismo , Membranas Sinápticas/metabolismo , Sinaptossomos/metabolismo , Animais , Bases de Dados de Proteínas , Eletroforese em Gel Bidimensional , Expressão Gênica , Filogenia , Proteínas/genética , Proteoma/genética , Proteômica , Nervo Radial/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Estrelas-do-Mar/genética , Membranas Sinápticas/genética , Sinaptossomos/químicaRESUMO
Serotonin (5-HT), an important molecule in metazoans, is involved in a range of biological processes including neurotransmission and neuromodulation. Both its creation and release are tightly regulated, as is its removal. Multiple neurochemical pathways are responsible for the catabolism of 5-HT and are phyla specific; therefore, by elucidating these catabolic pathways we glean greater understanding of the relationships and origins of various transmitter systems. Here, 5-HT catabolic pathways were studied in Strongylocentrotus purpuratus and Xenoturbella bocki, two organisms occupying distinct positions in deuterostomes. The 5-HT-related compounds detected in these organisms were compared with those reported in other phyla. In S. purpuratus, 5-HT-related metabolites include N-acetyl serotonin, gamma-glutamyl-serotonin and 5-hydroxyindole acetic acid; the quantity and type were found to vary based on the specific tissues analyzed. In addition to these compounds, varying levels of tryptamine were also seen. Upon addition of a 5-HT precursor and a monoamine oxidase inhibitor, 5-HT itself was detected. In similar experiments using X. bocki tissues, the 5-HT-related compounds found included 5-HT sulfate, gamma-glutamyl-serotonin and 5-hydroxyindole acetic acid, as well as 5-HT and tryptamine. The sea urchin metabolizes 5-HT in a manner similar to both gastropod mollusks, as evidenced by the detection of gamma-glutamyl-serotonin, and vertebrates, as indicated by the presence of 5-hydroxyindole acetic acid and N-acetyl serotonin. In contrast, 5-HT metabolism in X. bocki appears more similar to common protostome 5-HT catabolic pathways.
Assuntos
Equinodermos/metabolismo , Serotonina/metabolismo , Strongylocentrotus purpuratus/metabolismo , 5-Hidroxitriptofano/farmacologia , Animais , Clorgilina/farmacologia , Di-Hidroxifenilalanina/farmacologia , Equinodermos/efeitos dos fármacos , Fluorescência , Redes e Vias Metabólicas/efeitos dos fármacos , Especificidade de Órgãos/efeitos dos fármacos , Nervo Radial/efeitos dos fármacos , Nervo Radial/metabolismo , Serotonina/química , Strongylocentrotus purpuratus/efeitos dos fármacosRESUMO
OBJECTIVE: To investigate changes in axonal persistent Na(+) currents in patients with neuropathic pain and the effects of mexiletine, an analogue of lidocaine, on axonal excitability properties. METHODS: The technique of latent addition was used to estimate nodal persistent Na(+) currents in superficial radial sensory axons of 17 patients with neuropathic pain/paresthesias before and after mexiletine treatment. Brief hyperpolarizing conditioning currents were delivered, and threshold change at the conditioning-test interval of 0.2 ms was measured as an indicator of the magnitude of persistent Na(+) currents. RESULTS: Threshold changes at 0.2 ms in latent addition were greater in the neuropathic patients than in the normal controls (p<0.001). After mexiletine treatment, there was a reduction in clinical pain scores (p<0.001), associated with decreased threshold changes at 0.2 ms (p<0.001). CONCLUSIONS: In patients with neuropathy, nodal persistent Na(+) currents in large sensory fibers increase, and the abnormal currents can be suppressed by mexiletine. Pain reduction after mexiletine treatment raises the possibility that excessive Na(+) currents are also suppressed in small fibers mediating neuropathic pain. SIGNIFICANCE: Latent addition can be used for indirect in vivo monitoring of nodal Na(+) currents in large sensory fibers, and future studies using this approach in small fibers would provide new insights into the peripheral mechanism of neuropathic pain.
Assuntos
Axônios/metabolismo , Mexiletina/uso terapêutico , Neuralgia/fisiopatologia , Parestesia/fisiopatologia , Nervo Radial/metabolismo , Células Receptoras Sensoriais/metabolismo , Canais de Sódio/efeitos dos fármacos , Adulto , Idoso , Condutividade Elétrica , Fenômenos Eletrofisiológicos , Feminino , Humanos , Lidocaína/análogos & derivados , Masculino , Pessoa de Meia-Idade , Neuralgia/tratamento farmacológico , Limiar da Dor/efeitos dos fármacos , Parestesia/tratamento farmacológico , Canais de Sódio/metabolismoRESUMO
Gonad-stimulating substance-like molecule (GSSL) was isolated from the radial nerve of the sea cucumber, Apostichopus japonicus (Aj-GSSL), and its partial DNA and protein sequences were characterized. The smaller part of the molecule that also retains GSSL activity was estimated. Radial nerve extract (RNE) induced germinal vesicle breakdown (GVBD) at 3 mg/ml in 85% of immature ovarian oocytes. Similar intensity of GSSL activity to RNE was seen in a fraction that contained peptides between 3 kDa and 10 kDa (3-10 kDa-fraction) separated by ultrafiltlation membrane. MALDI-TOF MS analysis and silver-stained 18% SDS-PAGE slab gels identified a major peptide at around 4.6 kDa in a 3-10 kDa-fraction, and that was subjected to internal protein sequencing. The resulting 12-amino acid sequence was not found in the BLAST database to date. Immunohistochemistry using antiserum raised against the 12-amino acid peptide located the peptide to granular cells in the hyponeural part of the radial nerve and in the epineural sinus beneath the radial nerve. Sequence data was obtained using degenerate primers designed from the 12-amino acid sequence and 5 and 3 RACEs. These resulted in a 148 bp cDNA that coded a 43-amino acid sequence of H2N-VLSKQAHHHHHEGWSLPGVPAEIDDLAGNIDYNIFKEQREKIK-COOH. The synthetic 43-amino acid Aj-GSSL generated from this sequence induced GVBD in 50% of immature ovarian oocytes at 6 microM. An N-terminal 21-amino acid peptide of the synthetic partial Aj-GSSL (Aj-GSSL-P1) induced GVBD to 80% of immature ovarian oocytes at 12 microM. This indicated that Aj-GSSL-P1 is of sufficient length for GSSL activity.
Assuntos
Hormônios de Invertebrado/metabolismo , Neuropeptídeos/metabolismo , Pepinos-do-Mar/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Hormônios de Invertebrado/química , Hormônios de Invertebrado/isolamento & purificação , Dados de Sequência Molecular , Peso Molecular , Neuropeptídeos/química , Neuropeptídeos/isolamento & purificação , Nervo Radial/metabolismo , Pepinos-do-Mar/químicaRESUMO
The echinoderm nervous system is one of the least studied among invertebrates, partly because the tools available to study the neurobiology of this phylum are limited. We have now produced a monoclonal antibody (RN1) that labels a nervous system component of the sea cucumber Holothuria glaberrima. Western blots show that our antibody recognizes a major band of 66 kDa and a minor band of 53 kDa. Immunohistological experiments show that, in H. glaberrima, the antibody distinctly labels most of the known nervous system structures and some components that were previously unknown or little studied. A surprising finding was the labeling of nervous plexi within the connective tissue compartments of all organs studied. Double labeling with holothurian neuropeptides and an echinoderm synaptotagmin showed that RN1 labeled most, if not all, of the fibers labeled by these neuronal markers, but also a larger component of cells and fibers. The presence of a distinct connective tissue plexus in holothurians is highly significant since these organisms possess mutable connective tissues that change viscosity under the control of the nervous system. Therefore, the cells and fibers recognized by our monoclonal antibodies may be involved in controlling tensility changes in echinoderm connective tissue.
Assuntos
Tecido Conjuntivo/inervação , Holothuria/anatomia & histologia , Animais , Anticorpos Monoclonais/metabolismo , Antígenos/metabolismo , Epiderme/metabolismo , Holothuria/imunologia , Holothuria/metabolismo , Sistema Nervoso/anatomia & histologia , Sistema Nervoso/imunologia , Sistema Nervoso/metabolismo , Nervo Radial/metabolismoRESUMO
We documented expression of the pan-metazoan neurogenic gene engrailed in larval and juvenile Patiriella sea stars to determine if this gene patterns bilateral and radial echinoderm nervous systems. Engrailed homologues, containing conserved En protein domains, were cloned from the radial nerve cord. During development, engrailed was expressed in ectodermal (nervous system) and mesodermal (coeloms) derivatives. In larvae, engrailed was expressed in cells lining the larval and future adult coeloms. Engrailed was not expressed in the larval nervous system. As adult-specific developmental programs were switched on during metamorphosis, engrailed was expressed in the central nervous system and peripheral nervous system (PNS), paralleling the pattern of neuropeptide immunolocalisation. Engrailed was first seen in the developing nerve ring and appeared to be up-regulated as the nervous system developed. Expression of engrailed in the nerve plexus of the tube feet, the lobes of the hydrocoel along the adult arm axis, is similar to the reiterated pattern of expression seen in other animals. Engrailed expression in developing nervous tissue reflects its conserved role in neurogenesis, but its broad expression in the adult nervous system of Patiriella differs from the localised expression seen in other bilaterians. The role of engrailed in patterning repeated PNS structures indicates that it may be important in patterning the fivefold organisation of the ambulacrae, a defining feature of the Echinodermata.
Assuntos
Proteínas de Homeodomínio/metabolismo , Larva/crescimento & desenvolvimento , Nervo Radial/crescimento & desenvolvimento , Estrelas-do-Mar/crescimento & desenvolvimento , Sequência de Aminoácidos , Animais , Padronização Corporal , Sistema Nervoso Central/crescimento & desenvolvimento , Sistema Nervoso Central/metabolismo , Epitopos , Evolução Molecular , Marcadores Genéticos , Proteínas de Homeodomínio/genética , Imuno-Histoquímica , Larva/metabolismo , Metamorfose Biológica/fisiologia , Dados de Sequência Molecular , Sistema Nervoso Periférico/crescimento & desenvolvimento , Sistema Nervoso Periférico/metabolismo , Nervo Radial/embriologia , Nervo Radial/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Especificidade da Espécie , Estrelas-do-Mar/citologiaRESUMO
The sodium channels SNS/PN3 and NaN/SNS2 are regulated by the neurotrophic factors-nerve growth factor (NGF) and glial-derived neurotrophic factor (GDNF), and may play an important role in the development of pain after nerve injury or inflammation. These key molecules have been studied in an amputated causalgic finger and control tissues by immunohistochemistry. There was a marked increase in the number and intensity of SNS/PN3-immunoreactive nerve terminals in the affected finger, while GDNF-immunoreactivity was not observed, in contrast to controls. No differences were observed for NGF, trk A, NT-3 or NaN/SNS2-immunoreactivity. While further studies are required, these findings suggest that accumulation of SNS/PN3 and/or loss of GDNF may contribute to pain in causalgia, and that selective blockers of SNS/PN3 and/or rhGDNF may provide effective novel treatments.
Assuntos
Causalgia/metabolismo , Dedos/fisiopatologia , Fatores de Crescimento Neural , Proteínas do Tecido Nervoso/deficiência , Neurônios Aferentes/metabolismo , Neuropeptídeos/metabolismo , Nociceptores/metabolismo , Nervo Radial/metabolismo , Canais de Sódio/metabolismo , Idoso , Cotos de Amputação/patologia , Cotos de Amputação/fisiopatologia , Causalgia/fisiopatologia , Feminino , Dedos/inervação , Dedos/cirurgia , Fator Neurotrófico Derivado de Linhagem de Célula Glial , Humanos , Imuno-Histoquímica , Mecanorreceptores/metabolismo , Mecanorreceptores/patologia , Canal de Sódio Disparado por Voltagem NAV1.8 , Fibras Nervosas/metabolismo , Fibras Nervosas/patologia , Nociceptores/fisiopatologia , Complicações Pós-Operatórias/metabolismo , Complicações Pós-Operatórias/fisiopatologia , Nervo Radial/fisiopatologiaRESUMO
The possibility that brain phagocytes may empty remnants of degenerated neurons into capillaries has been studied in frogs. Degeneration of nerve fibers was brought about by transectioning the optic tract, the tectothalamic and tectoisthmic tracts, the postoptic commissure or the radial nerve. To help identification of phagocytozed degenerated neuronal elements, the transected fibers were filled either with horseradish peroxidase (HRP) or cobaltous-lysine complex. The survival times were 3, 4, 7, 27, 47 and 70 days after the application of the markers. The HRP-labeled structures were identified in 60 microm thick sections using diaminobenzidine as chromogen, while cobalt was precipitated in the form of cobaltous sulfide. Small pieces of these sections were further processed for electron microscopy. In each area of the brain and spinal cord investigated, microglial cells and astrocytic processes containing fragments of degenerated neuronal elements could be seen close to capillaries. In some cases a microglial or astrocytic process pierced the capillary basal lamina and seemingly delivered inclusion bodies into the cytoplasm of capillary endothelial cells and pericytes. In the inclusion bodies, which were usually large vesicles, fragments of HRP or cobalt-labeled or unlabeled membranes with a foamy appearance, or condensed myelin lamellae could be observed. These vesicles protruded the luminal membrane of the endothelial cell that was disrupted in some cases suggesting that the content of the inclusion body was discharged into the lumen of the capillary. These results give support to Penfield's hypothesis (1925) that glial cells may empty phagocytozed materials into capillaries.
Assuntos
Astrócitos/ultraestrutura , Encéfalo/ultraestrutura , Endotélio Vascular/ultraestrutura , Lisina/análogos & derivados , Microglia/ultraestrutura , Fagócitos/ultraestrutura , Fagocitose/fisiologia , Rana esculenta/anatomia & histologia , Animais , Astrócitos/metabolismo , Membrana Basal/metabolismo , Membrana Basal/ultraestrutura , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Citoplasma/metabolismo , Citoplasma/ultraestrutura , Endotélio Vascular/metabolismo , Peroxidase do Rábano Silvestre , Lisossomos/metabolismo , Lisossomos/ultraestrutura , Microglia/metabolismo , Microscopia Eletrônica , Bainha de Mielina/metabolismo , Bainha de Mielina/ultraestrutura , Vias Neurais/irrigação sanguínea , Vias Neurais/metabolismo , Vias Neurais/ultraestrutura , Neurônios/metabolismo , Neurônios/ultraestrutura , Fagócitos/metabolismo , Nervo Radial/irrigação sanguínea , Nervo Radial/metabolismo , Nervo Radial/ultraestrutura , Rana esculenta/metabolismo , Medula Espinal/irrigação sanguínea , Medula Espinal/metabolismo , Medula Espinal/ultraestrutura , Colículos Superiores/irrigação sanguínea , Colículos Superiores/metabolismo , Colículos Superiores/ultraestruturaRESUMO
The results of preliminary studies suggest that the cytoskeletal fraction of the radial nerve of the starfish Asterias amurensis contained a 32 kDa protein, which is tissue specific. This protein was isolated from the radial nerve by preparative electrophoresis and used as an antigen for raising polyclonal antibodies. When testing these antibodies on sections of the starfish tissues, it was shown that they interact only with the proteins present in the radial nerve cells. A conclusion was drawn that the raised antibodies may be used as a cell marker when studying regeneration of the nervous system in starfish.
Assuntos
Anticorpos , Proteínas do Citoesqueleto/imunologia , Proteínas do Tecido Nervoso/imunologia , Nervo Radial/metabolismo , Estrelas-do-Mar , Animais , Especificidade de Anticorpos , Proteínas do Citoesqueleto/isolamento & purificação , Proteínas do Citoesqueleto/metabolismo , Eletroforese em Gel de Poliacrilamida , Imuno-Histoquímica , Peso Molecular , Proteínas do Tecido Nervoso/isolamento & purificação , Proteínas do Tecido Nervoso/metabolismo , Nervo Radial/citologiaRESUMO
The spinal cord projections of the 3 main forelimb nerves-median, radial and ulnar, were studied in the rat dorsal horn with transganglionic transport of wheat germ agglutinin-horseradish peroxidase (WGA-HRP), or using the disappearance of fluoride resistant acid phosphatase (FRAP) after nerve section. The projection patterns in lamina II were similar following the two procedures. The median and the radial nerve fibers projected to the medial and the intermediate thirds, respectively, of the dorsal horn lamina II in spinal cord segments C4-C8. The ulnar nerve projected to segments C6-C8 between the areas occupied by the other two nerves. The FRAP method also showed that the lateral part of lamina II, which was not filled by radial nerve fibers, received the projections from the dorsal cutaneous branches of cervical spinal nerves. In addition, FRAP disappeared from the medial end of segment T1 after skin incisions extending from the medial brachium to the axilla, which seemed due to severance of the cutaneous branchlets of the lateral anterior thoracic nerve. The FRAP procedure is thus sensitive enough to detect fibers in lamina II arising from small peripheral nerves, and may be used as an alternative to the anterograde tracing methods whenever there are no overlapping projections.
Assuntos
Nervo Mediano/fisiologia , Nervo Radial/fisiologia , Medula Espinal/fisiologia , Transmissão Sináptica , Nervo Ulnar/fisiologia , Fosfatase Ácida/metabolismo , Animais , Transporte Biológico , Membro Anterior/inervação , Gânglios/metabolismo , Peroxidase do Rábano Silvestre , Masculino , Nervo Mediano/metabolismo , Sistema Nervoso/metabolismo , Fenômenos Fisiológicos do Sistema Nervoso , Nervo Radial/metabolismo , Ratos , Ratos Endogâmicos , Nervo Ulnar/metabolismo , Aglutininas do Germe de TrigoRESUMO
The axonal transport of vasoactive intestinal polypeptide (VIP) was examined in anesthetized cats. The distally directed (anterograde) flux of peptide was found to be about 35 fmol/h in the sciatic nerve. A smaller retrograde flux (8.5 fmol/h) weas also detected. In ulnar, radial and sciatic nerves, the average velocity of transport was calculated to be 2.5 mm/h in the anterograde and 0.6 mm/h in the retrograde direction. Clearance experiments indicated that the amounts of peptide available for transport in these two phases were 28% and 15% of the total, respectively. Estimates of true velocity based on these figures are 9 mm/h for anterograde transport and 4 mm/h for retrograde transport. Local injections of vinblastine were found to induce marked local increases in VIP-immunoreactivity, indicating that microtubules play a role in peptide transport. Subcellular distribution experiments showed that most of the transported VIP was associated with a particulate fraction, possibly corresponding to large vesicles. Only one molecular form of VIP-immuno-reactivity was detected by gel permeation chromatography and no evidence was obtained for cleavage of VIP precursors in the axon. Comparison of axonal flux of peptide with the apparent content of VIP in terminal regions indicated that the turnover time for this peptide is 5 days or longer in the periphery. The results are consistent with the view that peripheral neurons are dependent upon rapid axonal transport for the supply of vip to their terminals.