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1.
Ciênc. Anim. (Impr.) ; 32(2): 85-100, abr.-jun. 2022.
Artigo em Português | VETINDEX | ID: biblio-1402135

Resumo

O gato doméstico é a única espécie da família Felídea sem risco ou iminência de extinção, diferente da maior parte dos felinos selvagens. Desta forma, o desenvolvimento e aprimoramento de diferentes biotécnicas reprodutivas, são essenciais para a manutenção da qualidade reprodutiva, tendo em vista a preservação de espécies mais vulneráveis. Além disso, as biotécnicas do sêmen são para as tecnologias reprodutivas, como a inseminação artificial (IA) e a fertilização in vitro (FIV). Sendo assim, o objetivo deste compilado bibliográfico foi abordar as principais técnicas de colheita, análise e preservação de sêmen/espermatozoides felino, assim como o uso dessas células em IA e FIV. Para a colheita do sêmen felino, diferentes métodos têm sido aplicados: ejaculação farmacológica, eletroejaculação e vagina artificial. Em caso de óbito do reprodutor, os espermatozoides recuperados do epidídimo também apresentam viabilidade reprodutiva. Ademais, a cinética espermática avaliada pelo sistema CASA, a morfologia e a morfometria são as principais análises que demonstram a qualidade espermática e refletem na fertilidade do ejaculado. O sistema CASA também avalia a trajetória individual de cada espermatozoide, que ao se agrupar em clusters, demonstra a heterogeneidade do ejaculado nas subpopulações. Contudo, os diluentes para a conservação e refrigeração dos espermatozoides felinos e as curvas de congelação ainda não estão totalmente estabelecidos e influenciam diretamente a viabilidade dos espermatozoides criopreservados. Diante disso, os resultados da utilização do sêmen felino após criopreservação são inconsistentes, sendo necessários mais estudos para elucidar melhores curvas de congelação e meios de diluentes para viabilizar a preservação do material genético dos gatos.


The domestic cat is the only species of the Felidea family without risk or imminence of extinction, unlike most wild cats. Therefore, the development and improvement of different reproductive biotechnologies are essential for the maintenance of reproductive quality for the preservation of the most vulnerable species. Furthermore, semen biotechnologies are the basis for reproductive technologies such as artificial insemination (AI) and in vitro fertilization (IVF). Thus, the objective of this bibliographic compilation was to approach the main techniques of collection, analysis, and preservation of feline semen/sperm, as well as the use of these cells in AI and IVF. For feline semen collection, different methods have been applied: pharmacological ejaculation, electroejaculation, and artificial vagina. In case of death of the sire, sperm recovered from the epididymis also show reproductive viability. Moreover, the sperm kinetics evaluated by the CASA system, the morphology, and the morphometry are the main analyzes that demonstrate sperm quality and reflect on ejaculate fertility. The CASA system also evaluates the individual path of each sperm, which, when grouped into clusters, demonstrates the heterogeneity of the ejaculate in the subpopulations. However, diluents for the conservation and refrigeration of feline sperm and freezing curves are not yet fully established and directly influence the viability of cryopreserved sperm. Therefore, the results of using feline semen after cryopreservation are inconsistent, and further studies are needed to elucidate better freezing curves and diluents to enable the preservation of the genetic material of cats.


Assuntos
Animais , Masculino , Gatos , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Inseminação Artificial/veterinária , Fertilização in vitro/veterinária , Criopreservação/métodos , Recuperação Espermática/veterinária
2.
Semina ciênc. agrar ; 42(4): 2439-2452, jul.-ago. 2021. tab
Artigo em Inglês | VETINDEX | ID: biblio-1370921

Resumo

The present study aimed to evaluate the filtration for separating seminal plasma of boars' ejaculate by means of sperm viability and the occurrence of hyperactivation and lipid peroxidation in fresh semen and after cooling for up to 96 hours. The ejaculate of eight healthy boars of different breeds was collected and the gelatinous portion was separated and discarded. In the laboratory, the semen was fractioned into three aliquots (groups G1, G2 and G3) as follows: G1: semen with plasma diluted in BTS (TOTAL BTS); G2: semen centrifuged at 600G/10' (BTS CEN); and G3: semen filtered with the Sperm-filter® following dilution of the retained cells with BTS (BTS FIL). The analyses were performed at three moments: with fresh samples (D0) and after 48 (D2) and 96 hours (D4) of cooling at 17ºC. The kinetic evaluation was performed using the CASA system, which provided data for the classification of sperm hyperactivity. For lipid stress analysis, the TBARS (thiobarbituric acid reactive substance) test was performed. The variance analysis test was conducted to compare the results between the groups and moments analyzed. The results showed better total motility values (%) for G1 at D0 (67.9, P= 0.001), D2 (36.6, P= 0.004) and D4 (26.1, P= 0.003). The occurrence of hyperactivity was observed in G2 and G3 at moments D2 and D4. In addition, TBARS showed higher peroxidation levels for G1 at D0 (8.1 mM MDA/ml, P= 0.01), D2 (7.4 mM MDA/ml, P= 0.02), and D4 (6.41mMol MDA/ml, P= 0.008) when compared to G2 and G3. Since the filtration method did not demonstrate any damage to the sperm viability, the study concluded that sperm filtration is an accessible and valid tool to replace centrifugation.(AU)


O presente estudo objetivou avaliar a filtração como alternativa para a separação do plasma seminal de ejaculados suínos, ao considerar a viabilidade espermática por meio da ocorrência de hiperativação e peroxidação lipídica no sêmen fresco e após refrigeração por até 96 horas. O ejaculado, de oito cachaços saudáveis de diferentes raças, foram colhidos por meio da técnica da mão enluvada e porção gelatinosa foi separada e descartada. Em laboratório, o sêmen foi fracionado em três alíquotas (grupos G1, G2 e G3) da seguinte forma: G1: sêmen e plasma seminal, diluído em BTS (TOTAL BTS); G2: ejaculado centrifugado a 600G/10' para separação do plasma seminal, e o pellet de espermatozoides formados foram ressuspensos em BTS (BTS CEN); e G3: sêmen filtrado com Sperm-filter®, e espermatozoides retidos foram diluídos em BTS (BTS FIL). As análises foram realizadas em três momentos: amostras frescas (D0), após 48 horas (D2) e seguidas 96 horas (D4) de refrigeração a 17ºC. A avaliação cinética foi realizada pelo sistema CASA, que forneceu dados para a classificação da hiperatividade espermática. Para análise de estresse lipídico, foi realizado o teste TBARS (substâncias reagente ao ácido barbitúrico). Um teste de análise de variâncias foi feito para detectar diferenças entre os grupos e momentos avaliados. Os resultados mostraram melhores valores de motilidade total (%) para G1 em D0 (67,9, P = 0,001), D2 (36,6, P = 0,004) e D4 (26,1, P = 0,003). A ocorrência de hiperatividade foi observada em G2 e G3 nos momentos D2 e D4. Além disso, o TBARS mostrou níveis de peroxidação lipídica mais elevados para G1 em D0 (8,1 mM MDA / ml, P = 0,01), D2 (7,4 mM MDA/ml, P = 0,02) e D4 (6,41 mMol MDA / ml, P = 0,008) quando comparado com G2 e G3. Como a filtração não induziu a danos na viabilidade espermática, o estudo concluiu que a filtração espermática é uma ferramenta acessível e válida para substituir a centrifugação com intuito de separar o plasma seminal.(AU)


Assuntos
Animais , Masculino , Sêmen , Suínos , Peroxidação de Lipídeos
3.
Ciênc. Anim. (Impr.) ; 30(04, Supl. 2): 195-199, 2020.
Artigo em Português | VETINDEX | ID: biblio-1472560

Resumo

The aim of the study was to evaluate the influence of hyperactivated sperm kinetics on pregnancy rate in IATF. Two experimental groups were established, based on the results of the semen analysis in the CASA system: groups of bulls with hyperactivated sperm (N = 10; HIPER) and bulls with non-hyperactivated sperm (N = 14; N HIPER). Differences between groups were estimated by the t test, and a significance level <5% was considered. Highervalues for the variables were identified in the HIPER group: VAP; VSL; VCL; ALH; RAPID cells and SLOW cells. On the other hand: BCF; STR; LIN; WOB and MEDIUM cells, which had higher values in the N HIPER group. No difference was found for the pregnancy rate variable between groups (p=0.454). Therefore, although the CASA system is an objective method of analysis, we can consider that it alone is not sufficient to predict seminal fertility, but when associated with other techniques such as: specific software and multivariate statistics that identify subpopulations, if makes it an important methodology for assessing semen quality.


Assuntos
Masculino , Animais , Bovinos , Análise do Sêmen/veterinária , Espermatozoides/fisiologia , Cinética
4.
Ciênc. Anim. (Impr.) ; 30(04, Supl. 2): 214-218, 2020. tab
Artigo em Português | VETINDEX | ID: biblio-1472564

Resumo

Sperm hyperactivity is a physiological behavior, and in the feline species it is characterized by an increase in the curvilinear velocity (VCL) and a greater head beat (ALH) evaluated by the CASA system. The study aimed to compare and correlate kinetic parameters of Hyperactive and Non-Hyperactive feline ejaculates when considering the means of VCL and ALH. Ejaculates were collected by electro ejaculation from 21 cats. The seminal samples had the kinetic parameters evaluated by the CASA system. From the average values of VCL and ALH, the ejaculates were classified in group HP (Hyperactivated, when VCL>190.17µm/s and ALH>6.44µm) and NHP (Non-Hyperactivated, when VCL<190.17 and ALH<6.44 µm). A T test and Pearson's correlation were performed with a significance of p<0.05. Among the groups, were detected higher values of total (HP: 68,2% vs NHP: 35,9%) and progressive (HP:40,1% vs NPH: 17,18%) motility, VAP (HP: 165,85µm/s vs NHP: 97,72 µm/s), VSL (HP:137,63µm/s vs NHP 82,6µm/s), VCL (HP: 237,31µm/s vs NHP: 147,94µm/s) and ALH (HP: 7,28µm vs NHP:5,42µm) for the HP group. There was a correlation in the HP ejaculates between total and progressive motility. In the NHP group, a correlation was observed between motility and progressive motility, and between progressive motility and STR and LIN. It was concluded that HP spermatozoa have a higher curvilinear speed, while NHP spermatozoa stand out due to their straight path.


Assuntos
Masculino , Animais , Gatos , Espermatozoides/fisiologia , Gatos , Motilidade dos Espermatozoides/fisiologia
5.
Ci. Anim. ; 30(04, Supl. 2): 195-199, 2020.
Artigo em Português | VETINDEX | ID: vti-32343

Resumo

The aim of the study was to evaluate the influence of hyperactivated sperm kinetics on pregnancy rate in IATF. Two experimental groups were established, based on the results of the semen analysis in the CASA system: groups of bulls with hyperactivated sperm (N = 10; HIPER) and bulls with non-hyperactivated sperm (N = 14; N HIPER). Differences between groups were estimated by the t test, and a significance level <5% was considered. Highervalues for the variables were identified in the HIPER group: VAP; VSL; VCL; ALH; RAPID cells and SLOW cells. On the other hand: BCF; STR; LIN; WOB and MEDIUM cells, which had higher values in the N HIPER group. No difference was found for the pregnancy rate variable between groups (p=0.454). Therefore, although the CASA system is an objective method of analysis, we can consider that it alone is not sufficient to predict seminal fertility, but when associated with other techniques such as: specific software and multivariate statistics that identify subpopulations, if makes it an important methodology for assessing semen quality.(AU)


Assuntos
Animais , Masculino , Bovinos , Espermatozoides/fisiologia , Análise do Sêmen/veterinária , Cinética
6.
Ci. Anim. ; 30(04, Supl. 2): 214-218, 2020. tab
Artigo em Português | VETINDEX | ID: vti-32327

Resumo

Sperm hyperactivity is a physiological behavior, and in the feline species it is characterized by an increase in the curvilinear velocity (VCL) and a greater head beat (ALH) evaluated by the CASA system. The study aimed to compare and correlate kinetic parameters of Hyperactive and Non-Hyperactive feline ejaculates when considering the means of VCL and ALH. Ejaculates were collected by electro ejaculation from 21 cats. The seminal samples had the kinetic parameters evaluated by the CASA system. From the average values of VCL and ALH, the ejaculates were classified in group HP (Hyperactivated, when VCL>190.17µm/s and ALH>6.44µm) and NHP (Non-Hyperactivated, when VCL<190.17 and ALH<6.44 µm). A T test and Pearson's correlation were performed with a significance of p<0.05. Among the groups, were detected higher values of total (HP: 68,2% vs NHP: 35,9%) and progressive (HP:40,1% vs NPH: 17,18%) motility, VAP (HP: 165,85µm/s vs NHP: 97,72 µm/s), VSL (HP:137,63µm/s vs NHP 82,6µm/s), VCL (HP: 237,31µm/s vs NHP: 147,94µm/s) and ALH (HP: 7,28µm vs NHP:5,42µm) for the HP group. There was a correlation in the HP ejaculates between total and progressive motility. In the NHP group, a correlation was observed between motility and progressive motility, and between progressive motility and STR and LIN. It was concluded that HP spermatozoa have a higher curvilinear speed, while NHP spermatozoa stand out due to their straight path.(AU)


Assuntos
Animais , Masculino , Gatos , Motilidade dos Espermatozoides/fisiologia , Espermatozoides/fisiologia , Gatos
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