Resumo
Background: Bovine leukemia virus (VLB) is an oncogenic deltaretrovirus associated with the development of persistent lymphocytosis (LP) and lymphosarcomas in cattle. LP is characterized by chronic elevation of the number of circulating lymphocytes, in the case of B lymphocytes. Several studies have described functional changes in various leukocyte populations in both blood and milk in VLB-infected animals. The impact of some chronic diseases of low lethality is aggravated by the emergence of comorbidities.The objective of the present study was to evaluate the oxidative metabolism and neutrophil phagocytosis of bovines of the Holtein breed naturally infected with the bovine leukemia virus (VLB). Materials, Methods & Results: In this study, 20 cows were divided into three groups: (NG) seven non-seroreagent animals for VLB and without hematological alterations; (GAL) eight seroreagent animals for VLB and without hematological alterations; and (GLP) five seroreagent animals for VLB with persistent lymphocytosis (LP). The oxidative metabolism of neutrophils was determined by the tetrazolium nitroblast reduction test stimulated or not with Zymosan particles. The percentage of neutrophils that phagocytosed Zymosan particle (s) was also evaluated. The data were initially evaluated for normality and homoscedasticity by the Shapiro-Wilk test. Then the ANOVA test followed by the Student-Newman-Keuls test was applied for the comparison between the NG, GAL and GLP animals. Comparison between the NG animals and the seroreagent animals for the VLB (GVLB) was also performed through the unpaired Students t-test. The value of P < 0.05 was considered significant. No significant differences were observed in oxidative neutrophil metabolism in stimulated and non-stimulated samples with Zymosan particles nor in the percentage...
Assuntos
Feminino , Animais , Bovinos , Deltaretrovirus , Leucose Enzoótica Bovina , Neutrófilos , Vírus da Leucemia Bovina/imunologia , Espécies Reativas de Oxigênio , FagocitoseResumo
Brazil currently ranks as one of the world leaders in food production and exportation. This scenario encourages the development of animal and plant health programs to ensure the production of safe food, helping the country to become an international provider of food for excellence. However, some health problems in dairy production, such as mastitis, have garnered increasing concern. This study aimed to estimate the prevalence of bovine mastitis in select properties located in the western Santa Catarina region, to assess the susceptibility profile to antimicrobial agents used for treatment and to check for the presence of genes (icaA and icaD) associated with biofilm formation in Staphylococcus aureus. In 148 milk samples collected, 72.97% had bacterial growth (n = 108). Among the isolated microorganisms, 21.62% (n = 32) were classified as Staphylococcus aureus, 18.91% (n = 28) as Staphylococcus sp. coagulase negative, 7.43% (n = 11) as Corynebacterium sp., 6.76% (n = 10) as Staphylococcus sp. coagulase positive, 5.41% (n = 8) as Nocardia sp. and 12.83% (n = 19) classified in different bacterial genera. Among the isolates submitted for antimicrobial susceptibility testing, it was observed that 8.95% (n = 6/67) had resistance to amoxicillin, 8.04% (n = 7/87) to ampicillin, 5.88% (n = 5/85) to cephalothin, 3.40% (n = 3/88) to ceftiofur and enrofloxacin, 20.45% (n = 18/88) to strep...(AU)
O Brasil situa-se hoje como um dos líderes mundiais em produção e exportação de alimentos. Este cenário incita a elaboração de programas de sanidade animal e vegetal que garantam a produção de alimentos seguros, contribuindo para que o país se torne um fornecedor internacional de alimentos por excelência. Entretanto, alguns problemas sanitários na produção leiteira, como a mastite, têm acarretado crescentes preocupações. O presente estudo teve como objetivo estimar a prevalência de mastite bovina em algumas propriedades localizadas na região Oeste de Santa Catarina, avaliar o perfil de suscetibilidade aos antimicrobianos empregados para tratamento e verificar a presença de genes (icaA e icaD) associados à formação de biofilmes em Staphylococcus aureus. Em 148 amostras de leite coletadas, 72,97% apresentaram crescimento bacteriano (n=108). Dentre os micro-organismos isolados, 21,62% (n=32) foram classificados como Staphylococcus aureus, 18,91% (n=28) como Staphylococcus sp. coagulase negativa, 7,43% (n=11) como Corynebacterium sp., 6,76% (n=10) como Staphylococcus sp. coagulase positiva, 5,41% (n=8) como Nocardia sp. e 12,83% (n=19) classificados em diferentes gêneros bacterianos. Dentre os isolados submetidos ao teste de suscetibilidade aos antimicrobianos, observou-se 8,95% (n=6/67) de resistência à Amoxicilina, 8,04% (n=7/87) à Ampicilina, 5,88% (n=5/85) à Cefalotina, 3,40%...(AU)
Assuntos
Animais , Bovinos , Mastite Bovina/diagnóstico , Mastite Bovina/imunologia , Mastite Bovina/microbiologia , Anti-Infecciosos , Biofilmes , Staphylococcus aureusResumo
Brazil currently ranks as one of the world leaders in food production and exportation. This scenario encourages the development of animal and plant health programs to ensure the production of safe food, helping the country to become an international provider of food for excellence. However, some health problems in dairy production, such as mastitis, have garnered increasing concern. This study aimed to estimate the prevalence of bovine mastitis in select properties located in the western Santa Catarina region, to assess the susceptibility profile to antimicrobial agents used for treatment and to check for the presence of genes (icaA and icaD) associated with biofilm formation in Staphylococcus aureus. In 148 milk samples collected, 72.97% had bacterial growth (n = 108). Among the isolated microorganisms, 21.62% (n = 32) were classified as Staphylococcus aureus, 18.91% (n = 28) as Staphylococcus sp. coagulase negative, 7.43% (n = 11) as Corynebacterium sp., 6.76% (n = 10) as Staphylococcus sp. coagulase positive, 5.41% (n = 8) as Nocardia sp. and 12.83% (n = 19) classified in different bacterial genera. Among the isolates submitted for antimicrobial susceptibility testing, it was observed that 8.95% (n = 6/67) had resistance to amoxicillin, 8.04% (n = 7/87) to ampicillin, 5.88% (n = 5/85) to cephalothin, 3.40% (n = 3/88) to ceftiofur and enrofloxacin, 20.45% (n = 18/88) to strep...
O Brasil situa-se hoje como um dos líderes mundiais em produção e exportação de alimentos. Este cenário incita a elaboração de programas de sanidade animal e vegetal que garantam a produção de alimentos seguros, contribuindo para que o país se torne um fornecedor internacional de alimentos por excelência. Entretanto, alguns problemas sanitários na produção leiteira, como a mastite, têm acarretado crescentes preocupações. O presente estudo teve como objetivo estimar a prevalência de mastite bovina em algumas propriedades localizadas na região Oeste de Santa Catarina, avaliar o perfil de suscetibilidade aos antimicrobianos empregados para tratamento e verificar a presença de genes (icaA e icaD) associados à formação de biofilmes em Staphylococcus aureus. Em 148 amostras de leite coletadas, 72,97% apresentaram crescimento bacteriano (n=108). Dentre os micro-organismos isolados, 21,62% (n=32) foram classificados como Staphylococcus aureus, 18,91% (n=28) como Staphylococcus sp. coagulase negativa, 7,43% (n=11) como Corynebacterium sp., 6,76% (n=10) como Staphylococcus sp. coagulase positiva, 5,41% (n=8) como Nocardia sp. e 12,83% (n=19) classificados em diferentes gêneros bacterianos. Dentre os isolados submetidos ao teste de suscetibilidade aos antimicrobianos, observou-se 8,95% (n=6/67) de resistência à Amoxicilina, 8,04% (n=7/87) à Ampicilina, 5,88% (n=5/85) à Cefalotina, 3,40%...