Efeito da adição de angiotensina-(1-7) na taxa de estradiol de cabras submetidas a protocolo de sincronização do estro e ovulação / Effect of angiotensin (1-7) on estradiol rate of goats undergoing synchronization protocol of estrus and ovulation

This study evaluated the effect of angiotensin- (1-7) on the estradiol levels of goats submitted to aprotocol of synchronization of estrus and ovulation when applied close to the ovulatory period. The animalswere divided into two experimental groups: Control (n = 10) and Angiotensin- (1-7) (n = 10). All animals weresubmitted to estrus synchronization protocol and on days 12 and 13 the animals received the treatmentsaccording to the experimental groups and blood samples from the external jugular vein were collected every 12hours for estradiol dosing (E2) by the Elisa test. The results were submitted to analysis of variance followed bythe Dunnett's test (P <0.05). No increase in serum estradiol concentration was observed in any of thetreatments. It was concluded that the application of angiotensin- (1-7) during the preovulatory period, with onlytwo applications, was not sufficient to significantly increase estradiol levels.(AU)

Efeito da adição de angiotensina-(1-7) na taxa de estradiol de cabras submetidas a protocolo de sincronização do estro e ovulação / Effect of angiotensin (1-7) on estradiol rate of goats undergoing synchronization protocol of estrus and ovulation

This study evaluated the effect of angiotensin- (1-7) on the estradiol levels of goats submitted to aprotocol of synchronization of estrus and ovulation when applied close to the ovulatory period. The animalswere divided into two experimental groups: Control (n = 10) and Angiotensin- (1-7) (n = 10). All animals weresubmitted to estrus synchronization protocol and on days 12 and 13 the animals received the treatmentsaccording to the experimental groups and blood samples from the external jugular vein were collected every 12hours for estradiol dosing (E2) by the Elisa test. The results were submitted to analysis of variance followed bythe Dunnett's test (P <0.05). No increase in serum estradiol concentration was observed in any of thetreatments. It was concluded that the application of angiotensin- (1-7) during the preovulatory period, with onlytwo applications, was not sufficient to significantly increase estradiol levels.

Efeito de um inibidor da Enzima Conversora de Angiotensina sobre a esteroidogênese, e Angiotensina II e Angiotensina-(1-7) em cabras superovuladas / Effect of an angiotensin-converting enzyme inhibitor on steroidogenesis, angiotensin II and angiotensin-(1-7) in superovulated goats

Local renin angiotensin (RAS) system has been described in the ovary, which has been implicated invarious reproductive functions. To evaluate the ovarian RAS in, 13 goats were randomly divided into two groups:Enalapril (n = 7) and control (n = 6). Then, they received superovulation protocol. Enalapril further groupreceived subcutaneously (2mg/kg) enalapril maleate (0.4 mg/kg/day). Blood samples were collected on days 3, 6, 9and 11, and follicular fluid samples from pre-ovulatory ovarian follicles after exposure by celiotomy on D11. AngII in serum and follicular fluid were evaluated on days 9 and 12, Ang-(1-7) on day 12 by HPLC and RIA. E2 andP4 concentrations in serum were determined by ELISA. Ang-(1-7) concentrations in plasma was greater on day9 (P < 0.05), important period for the recruitment and follicular selection. This may indicate that these peptidescan an important function in follicular development and oocyte maturation in superovulated goats.(AU)

Efeito de um inibidor da Enzima Conversora de Angiotensina sobre a esteroidogênese, e Angiotensina II e Angiotensina-(1-7) em cabras superovuladas / Effect of an angiotensin-converting enzyme inhibitor on steroidogenesis, angiotensin II and angiotensin-(1-7) in superovulated goats

Local renin angiotensin (RAS) system has been described in the ovary, which has been implicated invarious reproductive functions. To evaluate the ovarian RAS in, 13 goats were randomly divided into two groups:Enalapril (n = 7) and control (n = 6). Then, they received superovulation protocol. Enalapril further groupreceived subcutaneously (2mg/kg) enalapril maleate (0.4 mg/kg/day). Blood samples were collected on days 3, 6, 9and 11, and follicular fluid samples from pre-ovulatory ovarian follicles after exposure by celiotomy on D11. AngII in serum and follicular fluid were evaluated on days 9 and 12, Ang-(1-7) on day 12 by HPLC and RIA. E2 andP4 concentrations in serum were determined by ELISA. Ang-(1-7) concentrations in plasma was greater on day9 (P < 0.05), important period for the recruitment and follicular selection. This may indicate that these peptidescan an important function in follicular development and oocyte maturation in superovulated goats.

Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.(AU)

Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.