Comparative analysis of histopathological alterations and immunohistochemistry in cattle for diagnosis of rabies

Comparative analysis of histopathological alterations and immunohistochemistry was performed on 15 brain samples of cow. In the present study, brain tissues were positive in 9 cases (60.0%), using polyclonal antiserum by immunohistochemistry and 7 cases (46.60%) were found positive for rabies by demonstration of Negri bodies. Hundred neurons per case were observed for Negri bodies; number of Negri bodies in positive neurons was counted and a comparison of IHC and histopathology was done. Number of neurons positive for rabies virus antigen per 100 neurons (900 neurons) by IHC were more (665) than H& E staining (344) and average number of Negri bodies per neuron detected by IHC were more (2.97) than histopathology (1.52), therefore, IHC was found to be more sensitive than histopathology.

Comparative analysis of histopathological alterations and immunohistochemistry in cattle for diagnosis of rabies

Comparative analysis of histopathological alterations and immunohistochemistry was performed on 15 brain samples of cow. In the present study, brain tissues were positive in 9 cases (60.0%), using polyclonal antiserum by immunohistochemistry and 7 cases (46.60%) were found positive for rabies by demonstration of Negri bodies. Hundred neurons per case were observed for Negri bodies; number of Negri bodies in positive neurons was counted and a comparison of IHC and histopathology was done. Number of neurons positive for rabies virus antigen per 100 neurons (900 neurons) by IHC were more (665) than H& E staining (344) and average number of Negri bodies per neuron detected by IHC were more (2.97) than histopathology (1.52), therefore, IHC was found to be more sensitive than histopathology.(AU)

Antemortem Diagnosis of Rabies From Skin: Comparison of nested RT-PCR with TaqMan real time PCR

Molecular techniques were adopted to diagnose rabies viral RNA from skin biopsy samples collected from 20 animals. Nested RT-PCR and TaqMan real time PCR techniques have successfully diagnosed rabies viral RNA in 9 and 11 skin samples, respectively. The sensitivity of both techniques was calculated in comparison to FAT applied on brain samples. Sensitivity of 76.47% was obtained with nested RT-PCR on skin biopsy samples while Taqman real time PCR revealed sensitivity of 92.85%. It was concluded that TaqMan real time PCR is a useful, specific, sensitive and better molecular approach for antemortem rabies diagnosis from skin samples of rabies suspected animals.(AU)