Antemortem Diagnosis of Rabies From Skin: Comparison of nested RT-PCR with TaqMan real time PCR
Bansal, Karan; Singh, Charan Kamal K; Ramneek, Ramneek; Sandhu, Bhupinder Singh; Deka, Deepak; Dandale, Mangesh; Sood, Naresh Kumar.
Braz. j. vet. pathol;
5(3): 116-119, nov. 2012. tab
Artigo
em Inglês
| VETINDEX
| ID: biblio-1397844
Resumo
Molecular techniques were adopted to diagnose rabies viral RNA from skin biopsy samples collected from 20 animals. Nested RT-PCR and TaqMan real time PCR techniques have successfully diagnosed rabies viral RNA in 9 and 11 skin samples, respectively. The sensitivity of both techniques was calculated in comparison to FAT applied on brain samples. Sensitivity of 76.47% was obtained with nested RT-PCR on skin biopsy samples while Taqman real time PCR revealed sensitivity of 92.85%. It was concluded that TaqMan real time PCR is a useful, specific, sensitive and better molecular approach for antemortem rabies diagnosis from skin samples of rabies suspected animals.(AU)
Biblioteca responsável:
BR68.1
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