Resumo
Salmonella infections are an important cause of mortality and morbidity in cattle, and subclinically infected animals arefrequently found. The microorganism may exhibit multiple antibiotic resistance and may be able to survive in harsh environmentssuch as the gallbladder. Salmonella Multiple Antibiotic Resistance (MAR) was detected in bile and gallbladder epitheliumfrom cattle slaughtered in a plant in southern state of Rio de Janeiro, Brazil, under sanitary conditions. The frequency ofresistance to each drug was not significantly different between bile and epithelium, except in the case of cefotaxime, whichshowed increased resistance in the bile. Aztreonam was the most effective antibiotic for inhibiting strain growth. Since theresults showed Salmonella spp. strains resistant to twelve antimicrobials, it denotes an alarming risk, therefore in cases ofinvasive infections in both human and animals there will be reduction of therapeutic options against the pathogen.
Resumo
Salmonella infections are an important cause of mortality and morbidity in cattle, and subclinically infected animals arefrequently found. The microorganism may exhibit multiple antibiotic resistance and may be able to survive in harsh environmentssuch as the gallbladder. Salmonella Multiple Antibiotic Resistance (MAR) was detected in bile and gallbladder epitheliumfrom cattle slaughtered in a plant in southern state of Rio de Janeiro, Brazil, under sanitary conditions. The frequency ofresistance to each drug was not significantly different between bile and epithelium, except in the case of cefotaxime, whichshowed increased resistance in the bile. Aztreonam was the most effective antibiotic for inhibiting strain growth. Since theresults showed Salmonella spp. strains resistant to twelve antimicrobials, it denotes an alarming risk, therefore in cases ofinvasive infections in both human and animals there will be reduction of therapeutic options against the pathogen.
Resumo
Os antígenos total e vesicular de Taenia crassiceps e soros-controle de bovinos positivos e negativos para a cisticercose foramcomparados por meio de alguns parâmetros de padronização do teste ELISA, visando avaliar o seu desempenho no diagnósticoda referida doença. Os parâmetros avaliados foram: diferentes concentrações dos antígenos, diluições de soros e conjugados,marcas de placa e substâncias bloqueadoras das placas. As diluições 1:25 de soro e 1:2.500 de conjugado para o antígenode líquido vesicular e 1:5.000 para o antígeno total, o leite desnatado e as maiores concentrações dos antígenos testadas (1,2 e 4 g/orifício) foram os critérios de padronização que proporcionaram maior diferenciação entre soros positivos e negativos,conseqüentemente, melhor desempenho do teste ELISA.
Resumo
Pathogenic microorganisms can reside transiently or permanently in the gallbladder of cattle. Thus, during slaughter, more attention should be given to the gastrointestinal tract, especially to the accessory organ, the gallbladder. The main aim of this study was to characterize the bacterial microbiota present in bile and gallbladder epithelium of cattle slaughtered in a slaughtering plant under sanitary conditions. Thirty gallbladders intact were collected and researched in bile and epithelium the presence of Aerobic Mesophilic Heterotrophic Bacteria (AMHB), Staphylococcus spp., total Enterobacteriaceae, Enterococcus spp. and Salmonella spp. The frequency of isolation of the microorganism mentioned above were, respectively: 23.02%, 14.39%, 13.67%, 24.46%, 0% and 24.46%. Concerning both environments of the gallbladder, the frequency of isolation of the microorganisms in the epithelium was 64.03%, and in the bile 35.97%, with no statistical difference, but with significant difference between the population averages. In antimicrobial susceptibility testing, Staphylococcus strains from both bile and gallbladder epithelium showed sensitivity to the antimicrobials: penicillin G, ceftriaxone, chloramphenicol and gentamicin. The observation that gallbladder supports a high frequency of microorganisms brings us to the possible fact that cattle might be a persistent carrier of pathogens