Utilização da pele de tilápia-do-Nilo (Oreochromis niloticus) como xenoenxerto em um equino com ferimento traumático / Treatment of a traumatic equine wound using Nile tilapia (Oreochromis niloticus) skin as a xenograft

Background: Wounds are disruptions of the normal continuity of anatomic structures, generally due to local trauma. Theyare extremely prevalent in animals, especially horses, and a common reason for seeking veterinary attention. Their management aims to restore the function and integrity of the affected area in the shortest possible time and cost, while providingsatisfactory cosmetic results. This task becomes challenging when working with horses, considering the contact betweenwounds and contaminated environment is common. Thus, the present study aims to report the case of a traumatic equinewound treated with Nile Tilapia Fish Skin (NTFS).Case: A male 27-year-old horse previously castrated, with no defined breed (NDB), and weighing 400 kg presented a 6.0 x5.5 cm superficial wound in the distal left anterior limb (LAL) due to skin laceration. The animal belonged to the cavalry ofthe Military Police of Ceará, a public institution in Fortaleza, Brazil. Although in excellent general health, with no previouscomorbidities or restriction of movement, the animal was removed from its role in equine-assisted therapy (EAT) until completewound healing, aiming adequate evaluation of the novel biomaterial via lower influence of external factors. After informedconsent from the owner was obtained, Nile Tilapia Fish Skin was applied to the lesion. The Ethics Committee on the use ofanimals of the Drug Research and Development Center of the Federal University of Ceará, Fortaleza, Brazil, approved the studyprotocol. Compliance with regulations on the ethical treatment of animals was performed. Nile Tilapia Fish Skin applicationfollowed a protocol similar to that established in human clinical studies. Initially, the horse was submitted to wound cleaningwith tap water and 2% chlorhexidine gluconate, with no requirement of pre-treatment surgical...

Utilização da pele de tilápia-do-Nilo (Oreochromis niloticus) como xenoenxerto em um equino com ferimento traumático / Treatment of a traumatic equine wound using Nile tilapia (Oreochromis niloticus) skin as a xenograft

Background: Wounds are disruptions of the normal continuity of anatomic structures, generally due to local trauma. Theyare extremely prevalent in animals, especially horses, and a common reason for seeking veterinary attention. Their management aims to restore the function and integrity of the affected area in the shortest possible time and cost, while providingsatisfactory cosmetic results. This task becomes challenging when working with horses, considering the contact betweenwounds and contaminated environment is common. Thus, the present study aims to report the case of a traumatic equinewound treated with Nile Tilapia Fish Skin (NTFS).Case: A male 27-year-old horse previously castrated, with no defined breed (NDB), and weighing 400 kg presented a 6.0 x5.5 cm superficial wound in the distal left anterior limb (LAL) due to skin laceration. The animal belonged to the cavalry ofthe Military Police of Ceará, a public institution in Fortaleza, Brazil. Although in excellent general health, with no previouscomorbidities or restriction of movement, the animal was removed from its role in equine-assisted therapy (EAT) until completewound healing, aiming adequate evaluation of the novel biomaterial via lower influence of external factors. After informedconsent from the owner was obtained, Nile Tilapia Fish Skin was applied to the lesion. The Ethics Committee on the use ofanimals of the Drug Research and Development Center of the Federal University of Ceará, Fortaleza, Brazil, approved the studyprotocol. Compliance with regulations on the ethical treatment of animals was performed. Nile Tilapia Fish Skin applicationfollowed a protocol similar to that established in human clinical studies. Initially, the horse was submitted to wound cleaningwith tap water and 2% chlorhexidine gluconate, with no requirement of pre-treatment surgical...(AU)

Efeito do treinamento de doma sobre os parâmetros físicos e hematobioquímicos de equinos, antes e após o exercício / Effect of taming training on physical and hemato-biochemical parameters of horses before and after exercise

Os equinos são preparados para exercícios de alta intensidade e destacam-se em atividades físicas onde a velocidade e a resistência são fatores importantes. Contudo, poucos estudos relatam os impactos do treinamento de doma sobre a homeostase. Diante disso, objetivou-se avaliar o efeito do treinamento de doma sobre parâmetros físicos, hematológicos e bioquímicos em equinos. Para tanto, foram acompanhados cavalos (n =10) previamente selecionados para o programa de doma preparatória para o serviço de policiamento montado do Esquadrão de Polícia Montada (EPMONT, CE). Avaliaram-se as frequências cardíaca (FC) e respiratória (FR) e a temperatura e, em amostras de sangue coletadas, os parâmetros hematológicos e bioquímicos, nas seguintes etapas: em repouso (D0) e em treinamento, sendo no primeiro dia (D1), três meses após D1, no primeiro dia da fase de rua (D2), 15 (D3) e 30 (D4) dias, após o início da fase de rua. Para cada dia, o animal foi observado e amostras de sangue foram colhidas antes e depois do treinamento. Os resultados foram expressos em média ± erro padrão. Os dados foram analisados ao nível de 5% de significância (p≤0,05). Foram observados aumentos nas FC, FR e temperatura corporal, bem como nos teores de hemácias, hemoglobina, hematócrito, leucócitos totais, uréia, creatinina, aspartato-aminotransferase, lactato-desidrogenase, creatina-quinase, fosfatase alcalina e albumina depois do exercício, quando comparados a antes do treino. Conclui-se que o treinamento de doma em equinos provoca alterações importantes em parâmetros físicos, hematológicos e bioquímicos de cavalos, demonstrando a necessidade de acompanhamento dos animais.(AU)

Horses are animals naturally prepared for high-intensity exercise, especially in physical activities where speed and endurance are important factors. However, few studies have reported the impact of taming training in homeostasis. Thus, it was evaluate the effect of taming training horses in hematological, biochemical and physiological parameters. Horses (n =10) previously selected for the preparatory taming program of mounted police service of Esquadrão de Polícia Montada (EPMONT, CE). Were evaluate heart (HR) and respiratory (RR) rate, temperature (TºC) and in blood samples collected to access hematological and biochemical parameters on the fallow steps: animals on a rest day (D0) and during the training, at first day (D1), three months after D1, the first (D2), 15 (D3) and 30 (D4) days after early phase of the street. For each day, the animals were observed and blood samples were taken before and after training. Data were expressed as mean ± standard error and were analyzed with p≤0.05. The results present increased of HR, RR, T, counts of red blood cells and leukocytes and the levels of hemoglobin and hematocrit, urea, creatinine, aspartate aminotransferase, lactate dehydrogenase, creatine kinase, alkaline phosphatase and albumin after exercise when compared to before the exercise. In conclusion, the taming training causes changes in physics, hematological and biochemical parameters of horses, demonstrating to need have follow up during the exercise.(AU)

Efeito do treinamento de doma sobre os parâmetros físicos e hematobioquímicos de equinos, antes e após o exercício / Effect of taming training on physical and hemato-biochemical parameters of horses before and after exercise

Os equinos são preparados para exercícios de alta intensidade e destacam-se em atividades físicas onde a velocidade e a resistência são fatores importantes. Contudo, poucos estudos relatam os impactos do treinamento de doma sobre a homeostase. Diante disso, objetivou-se avaliar o efeito do treinamento de doma sobre parâmetros físicos, hematológicos e bioquímicos em equinos. Para tanto, foram acompanhados cavalos (n =10) previamente selecionados para o programa de doma preparatória para o serviço de policiamento montado do Esquadrão de Polícia Montada (EPMONT, CE). Avaliaram-se as frequências cardíaca (FC) e respiratória (FR) e a temperatura e, em amostras de sangue coletadas, os parâmetros hematológicos e bioquímicos, nas seguintes etapas: em repouso (D0) e em treinamento, sendo no primeiro dia (D1), três meses após D1, no primeiro dia da fase de rua (D2), 15 (D3) e 30 (D4) dias, após o início da fase de rua. Para cada dia, o animal foi observado e amostras de sangue foram colhidas antes e depois do treinamento. Os resultados foram expressos em média ± erro padrão. Os dados foram analisados ao nível de 5% de significância (p≤0,05). Foram observados aumentos nas FC, FR e temperatura corporal, bem como nos teores de hemácias, hemoglobina, hematócrito, leucócitos totais, uréia, creatinina, aspartato-aminotransferase, lactato-desidrogenase, creatina-quinase, fosfatase alcalina e albumina depois do exercício, quando comparados a antes do treino. Conclui-se que o treinamento de doma em equinos provoca alterações importantes em parâmetros físicos, hematológicos e bioquímicos de cavalos, demonstrando a necessidade de acompanhamento dos animais.

Horses are animals naturally prepared for high-intensity exercise, especially in physical activities where speed and endurance are important factors. However, few studies have reported the impact of taming training in homeostasis. Thus, it was evaluate the effect of taming training horses in hematological, biochemical and physiological parameters. Horses (n =10) previously selected for the preparatory taming program of mounted police service of Esquadrão de Polícia Montada (EPMONT, CE). Were evaluate heart (HR) and respiratory (RR) rate, temperature (TºC) and in blood samples collected to access hematological and biochemical parameters on the fallow steps: animals on a rest day (D0) and during the training, at first day (D1), three months after D1, the first (D2), 15 (D3) and 30 (D4) days after early phase of the street. For each day, the animals were observed and blood samples were taken before and after training. Data were expressed as mean ± standard error and were analyzed with p≤0.05. The results present increased of HR, RR, T, counts of red blood cells and leukocytes and the levels of hemoglobin and hematocrit, urea, creatinine, aspartate aminotransferase, lactate dehydrogenase, creatine kinase, alkaline phosphatase and albumin after exercise when compared to before the exercise. In conclusion, the taming training causes changes in physics, hematological and biochemical parameters of horses, demonstrating to need have follow up during the exercise.

A clinical trial and oral wound treated by essential oil of Lippia sidoides mouthrinse in horses

Background: Frequently the equine oral cavity is target of dental alterations, irregular wear of the dentition, tartar accumulation, gums inflammation, and other oral mucosa lesions. Therapeutically, active plants have been proposed to actpreventively against oral infections. The objective of this study was to evaluate a mouthrinse prepared with Lippia sidoidesCham. essential oil in equine dental diseases and oral wound healing treatment.Materials, Methods & Results: The experimental protocol was approved by the Ethics Committee of the Use of Animals(CEUA) of the State University of Ceara (UECE), n° 1158255-0/75. Mouthrinse was prepared with essential oil extractedfrom leaves of L. sidoides (EOLS). Thymol was the main constituent of the oil, up to 70%. Horses were chosen basedon clinical and oral examination. Dental calculus and gingivitis grade from incisor and canine teeth were scored duringevaluation. Two groups were used; OELS group (n = 10), and control group (n = 6), treated with mouthwash without EO(MR). A surgical scar (0.5 x 2 cm) was made near tooth 106 with the animals sedated. The lesions were observed on days 0(D0), 3 (D3), 7 (D7), and 14 (D14) after treatment that was applied once in 48 h for 14 days. Recovery of oral mucosa was,blindly, evaluated in tissue samples. EOLS treatment reduced teeth calculus scores from D0 to D14 (P ≤ 0.05). In relationto histological parameters, EOLS treatment induced discreet proliferation of fibroblasts and a moderated inflammation onD3; moderated proliferation of fibroblasts and moderated inflammation, intense angiogenesis and re-epithelization on D7,and intense fibroblast proliferation, intense angiogenesis, and reduced inflammation with complete...(AU)

A clinical trial and oral wound treated by essential oil of Lippia sidoides mouthrinse in horses

Background: Frequently the equine oral cavity is target of dental alterations, irregular wear of the dentition, tartar accumulation, gums inflammation, and other oral mucosa lesions. Therapeutically, active plants have been proposed to actpreventively against oral infections. The objective of this study was to evaluate a mouthrinse prepared with Lippia sidoidesCham. essential oil in equine dental diseases and oral wound healing treatment.Materials, Methods & Results: The experimental protocol was approved by the Ethics Committee of the Use of Animals(CEUA) of the State University of Ceara (UECE), n° 1158255-0/75. Mouthrinse was prepared with essential oil extractedfrom leaves of L. sidoides (EOLS). Thymol was the main constituent of the oil, up to 70%. Horses were chosen basedon clinical and oral examination. Dental calculus and gingivitis grade from incisor and canine teeth were scored duringevaluation. Two groups were used; OELS group (n = 10), and control group (n = 6), treated with mouthwash without EO(MR). A surgical scar (0.5 x 2 cm) was made near tooth 106 with the animals sedated. The lesions were observed on days 0(D0), 3 (D3), 7 (D7), and 14 (D14) after treatment that was applied once in 48 h for 14 days. Recovery of oral mucosa was,blindly, evaluated in tissue samples. EOLS treatment reduced teeth calculus scores from D0 to D14 (P ≤ 0.05). In relationto histological parameters, EOLS treatment induced discreet proliferation of fibroblasts and a moderated inflammation onD3; moderated proliferation of fibroblasts and moderated inflammation, intense angiogenesis and re-epithelization on D7,and intense fibroblast proliferation, intense angiogenesis, and reduced inflammation with complete...