Antimicrobial susceptibility of Salmonella serovars isolated from edible offal and carcasses of slaughtered poultry in the state of Tocantins, Brazil / Susceptibilidade antimicrobiana de sorovares de Salmonella sp. isolados de vísceras comestíveis e carcaças de aves abatidas no estado do Tocantins, Brasil

The aim of this study was to evaluate the profile of antibiotic resistance in Salmonella isolated from the carcasses, livers and hearts of chickens slaughtered in the state of Tocantins, Brazil, as recommended by the Normative Instruction 70 of 2003 of the Ministry of Agriculture, Livestock and Food Supply and the National Monitoring Program Prevalence and Bacterial Resistance in Chicken. Carcasses, livers and hearts from chicken with or without pericarditis/perihepatitis were studied in 60 lots of poultry slaughtered under the Federal Inspection System in the state of Tocantins, Brazil between August 2010 and June 2011. Twenty-six indicative Salmonella sp. were isolated in 11 lots (18.33%). Different strains of Salmonella were isolated more than a kind of sample/lot. The most frequent serovar was Enteriditis (38.46%, 10/26), while the second was Mbandaka (19.23%, 5/26), both isolated from hearts, livers and carcasses. Regarding antimicrobial resistance, of 12 tested principal pharmacological agents, the samples appeared to be most sensitive to tetracyclines, but showed 100% resistance to one or more active principal agents, especially sulfamethoxazole (30 mcg) and amoxicillin/clavulanic acid (30 mcg). Although Salmonella sp. was isolated from normal carcasses, the results are within permitted levels for unfrozen products according to Brazilian legislation. However, one should...(AU)

Com o objetivo de observar os parâmetros estabelecidos pela Instrução Normativa 70 de 2003 do Ministério da Agricultura Pecuária e Abastecimento, juntamente com o Programa Nacional de Monitoramento da Prevalência e da Resistência Bacteriana em Frango, o qual prevê o monitoramento de Salmonella sp. em produtos de frangos resfriados, foram estudadas carcaças e miúdos comestíveis (fígados e coração), condenados ou não por pericardite/perihepatite, em 60 lotes de aves abatidos sob Sistema de Inspeção Federal no estado do Tocantins, entre agosto de 2010 a junho de 2011. Foram isoladas 26 amostras indicativas de Salmonella sp. em 11 lotes (18,33%), sendo mais de uma estirpe por tipo de amostra. Os sorovares de maior frequência foram o Enteriditis (38,46%; 10/26) e Mbandaka (19,23%; 5/26), ambos isolados de coração, fígado e carcaça. Quanto ao perfil de resistência aos antimicrobianos, foram testados 12 princípios farmacológicos e as amostras apresentaram-se diferenciadas em alguns aspectos do encontrado na literatura consultada, sendo a maioria sensível às tetraciclinas, porém apresentaram 100% de resistência a um ou mais principio ativo, principalmente para Sulfamethoxazole (30 mcg) e Amoxicilina/Ácido Clavulânico (30 mcg). Apesar da Salmonella sp. ter sido isolada em carcaças normais, os resultados encontram-se dentro do permitido pela legislação brasileira vigente para produtos...(AU)

Deaths of Cows During Long Distance Transportation to the Slaughterhouse. Changes on Histopathological Examination, Muscle and Liver Glycogen Storage and Muscle pH

The present experiment aimed to perform histopathological, muscle and liver glycogen and muscle pH analyses ofcows dead during transportation, and submitted to necropsy in slaughterhouses inspected by Federal Agencies, in Araguaína– TO, Brazil, from January to July 2013. Six affected animals dead during transportation and submitted to necropsyconstituted the Experimental Group (EG); a Control Group (CG), was composed by 6 cows slaughtered ordinarily followingthe slaughter flow. Gross and histopathological evaluations were accomplished only on EG. The main gross lesionsobserved at necropsy were extensive subcutaneous and muscle hemorrhage and hematomas, interstitial (83.3% of cases) andalveolar (66.6% of cases) lung emphysema, lung congestion (66.6% of cases) and edema (16.6% of cases), kidney (83.3%of cases) and liver congestion (16.6% of cases). On histopathological evaluation, the lungs showed interstitial (100% ofcases) and alveolar emphysema (66.6% of cases), congestion (66.6% of cases), edema (16.6% of cases) and blood aspiration(16.6% of cases). Renal congestion (83.3% of cases), hyaline casts (50% of cases) and tubular degeneration were thekidneys changes (16.6% of cases). Rarefaction of the white pulp (66.6% of cases), red pulp (66.6% of cases) andhemosiderin (16.6% of cases) were detected on spleen. The hepatocytes showed turve and finely vacuolyzed cytoplasm(lace-like aspect) possibly caused by a reduction on glycogen (50% of cases) and congestion (16.6% of cases). Autholysiswas observed on 50% of the livers and 16.6% of the spleens and kidneys. The animals from the EG presented higher meanof degenerate muscle fibers. The intercostal muscle was more affected when compared with other muscles on the samegroup. Muscle pH values were higher on EG than on CG. Differently, glycogen average values were similar between groups(EG 58.97 µmol/g and CG 57.05 µmol/g)[...]

Deaths of Cows During Long Distance Transportation to the Slaughterhouse. Changes on Histopathological Examination, Muscle and Liver Glycogen Storage and Muscle pH

The present experiment aimed to perform histopathological, muscle and liver glycogen and muscle pH analyses ofcows dead during transportation, and submitted to necropsy in slaughterhouses inspected by Federal Agencies, in AraguaínaTO, Brazil, from January to July 2013. Six affected animals dead during transportation and submitted to necropsyconstituted the Experimental Group (EG); a Control Group (CG), was composed by 6 cows slaughtered ordinarily followingthe slaughter flow. Gross and histopathological evaluations were accomplished only on EG. The main gross lesionsobserved at necropsy were extensive subcutaneous and muscle hemorrhage and hematomas, interstitial (83.3% of cases) andalveolar (66.6% of cases) lung emphysema, lung congestion (66.6% of cases) and edema (16.6% of cases), kidney (83.3%of cases) and liver congestion (16.6% of cases). On histopathological evaluation, the lungs showed interstitial (100% ofcases) and alveolar emphysema (66.6% of cases), congestion (66.6% of cases), edema (16.6% of cases) and blood aspiration(16.6% of cases). Renal congestion (83.3% of cases), hyaline casts (50% of cases) and tubular degeneration were thekidneys changes (16.6% of cases). Rarefaction of the white pulp (66.6% of cases), red pulp (66.6% of cases) andhemosiderin (16.6% of cases) were detected on spleen. The hepatocytes showed turve and finely vacuolyzed cytoplasm(lace-like aspect) possibly caused by a reduction on glycogen (50% of cases) and congestion (16.6% of cases). Autholysiswas observed on 50% of the livers and 16.6% of the spleens and kidneys. The animals from the EG presented higher meanof degenerate muscle fibers. The intercostal muscle was more affected when compared with other muscles on the samegroup. Muscle pH values were higher on EG than on CG. Differently, glycogen average values were similar between groups(EG 58.97 µmol/g and CG 57.05 µmol/g)[...](AU)

Procedimentos operacionais de rastreabilidade e rotulagem para exportação de carne bovina / Traceability and labeling for beef exports

Com o aumento das exigências dos consumidores por produtos de qualidade e que garantam a inocuidade do mesmo, o processo de rotulagem juntamente com a rastreabilidade passaram a ser uma forma de garantir ao consumidor informações adequadas e pertinentes ao produto industrializado. E, para que estas informações estejam corretas é necessário o entendimento da legislação em vigor que regulamenta estes processos. Assim, o objetivo do presente estudo foi compilar as principais etapas necessárias para a rastreabilidade e rotulagem de produtos de origem bovina.

Due to consumers increasing demands for safe products, the labeling process and traceability has become a way of ensuring adequate consumer information and relevant to industrial product. And for theaccuracy of this information, it is necessary to understand the existing regulations that control these processes. So, the objective of the present study was to compile the major steps needed for traceability and labeling of beef products.

Antimicrobial susceptibility of Salmonella serovars isolated from edible offal and carcasses of slaughtered poultry in the state of Tocantins, Brazil / Susceptibilidade antimicrobiana de sorovares de Salmonella sp. isolados de vísceras comestíveis e carcaças de aves abatidas no estado do Tocantins, Brasil

The aim of this study was to evaluate the profile of antibiotic resistance in Salmonella isolated from the carcasses, livers and hearts of chickens slaughtered in the state of Tocantins, Brazil, as recommended by the Normative Instruction 70 of 2003 of the Ministry of Agriculture, Livestock and Food Supply and the National Monitoring Program Prevalence and Bacterial Resistance in Chicken. Carcasses, livers and hearts from chicken with or without pericarditis/perihepatitis were studied in 60 lots of poultry slaughtered under the Federal Inspection System in the state of Tocantins, Brazil between August 2010 and June 2011. Twenty-six indicative Salmonella sp. were isolated in 11 lots (18.33%). Different strains of Salmonella were isolated more than a kind of sample/lot. The most frequent serovar was Enteriditis (38.46%, 10/26), while the second was Mbandaka (19.23%, 5/26), both isolated from hearts, livers and carcasses. Regarding antimicrobial resistance, of 12 tested principal pharmacological agents, the samples appeared to be most sensitive to tetracyclines, but showed 100% resistance to one or more active principal agents, especially sulfamethoxazole (30 mcg) and amoxicillin/clavulanic acid (30 mcg). Although Salmonella sp. was isolated from normal carcasses, the results are within permitted levels for unfrozen products according to Brazilian legislation. However, one should...

Com o objetivo de observar os parâmetros estabelecidos pela Instrução Normativa 70 de 2003 do Ministério da Agricultura Pecuária e Abastecimento, juntamente com o Programa Nacional de Monitoramento da Prevalência e da Resistência Bacteriana em Frango, o qual prevê o monitoramento de Salmonella sp. em produtos de frangos resfriados, foram estudadas carcaças e miúdos comestíveis (fígados e coração), condenados ou não por pericardite/perihepatite, em 60 lotes de aves abatidos sob Sistema de Inspeção Federal no estado do Tocantins, entre agosto de 2010 a junho de 2011. Foram isoladas 26 amostras indicativas de Salmonella sp. em 11 lotes (18,33%), sendo mais de uma estirpe por tipo de amostra. Os sorovares de maior frequência foram o Enteriditis (38,46%; 10/26) e Mbandaka (19,23%; 5/26), ambos isolados de coração, fígado e carcaça. Quanto ao perfil de resistência aos antimicrobianos, foram testados 12 princípios farmacológicos e as amostras apresentaram-se diferenciadas em alguns aspectos do encontrado na literatura consultada, sendo a maioria sensível às tetraciclinas, porém apresentaram 100% de resistência a um ou mais principio ativo, principalmente para Sulfamethoxazole (30 mcg) e Amoxicilina/Ácido Clavulânico (30 mcg). Apesar da Salmonella sp. ter sido isolada em carcaças normais, os resultados encontram-se dentro do permitido pela legislação brasileira vigente para produtos...