Extended-Spectrum Beta-Lactamase Producing Strains of Escherichia Coli Isolated from Avian Cellulitis Lesions

Avian cellulitis causes significant losses to the poultry industry. Avian-pathogenic Escherichia coli (APEC) is the etiological agent of that disease. This microorganism has zoonotic potential and may act as reservoir of antimicrobial-resistance genes. In this context, the production of extended-spectrum B-lactamase (ESBL) is one of the main antimicrobial resistance mechanisms. The objective of this study was to determine the production of ESBL in an Escherichia coli (E. coli) strain isolated from avian cellulitis lesions. Twenty-two E. Coli isolates were harvested from cellulitis lesions in chicken carcasses in a commercial processing plant. Isolates were then submitted to virulence genotypic profile (iutA, hlyF, iss, ironN, ompT) analysis, antimicrobial susceptibility test, and detection of ESBL production. The results showed that 22.7% of the isolates presented five virulence genes, 9.1% four genes, 36.4% three genes, 13.6% two genes, and 18.2% one gene. The tested isolates showed resistance to ampicillin (90.9%), ceftiofur (54.5%), gentamicin (45.5%), tetracycline (72.1%), sulfamethoxazole/trimethoprim (54.5%), and enrofloxacin (54.5%). Furthermore, 77.3% of the isolates presented multidrug resistance (MDR) profile and 72.7% were positive for ESBL production. This study is the first description of ESBL-producing APEC isolated from avian cellulitis lesions, which suggests the need to establish efficient APEC control measures and programs to prevent flock productivity losses due to colibacillosis and public health risks.(AU)

Extended-Spectrum Beta-Lactamase Producing Strains of Escherichia Coli Isolated from Avian Cellulitis Lesions

Avian cellulitis causes significant losses to the poultry industry. Avian-pathogenic Escherichia coli (APEC) is the etiological agent of that disease. This microorganism has zoonotic potential and may act as reservoir of antimicrobial-resistance genes. In this context, the production of extended-spectrum B-lactamase (ESBL) is one of the main antimicrobial resistance mechanisms. The objective of this study was to determine the production of ESBL in an Escherichia coli (E. coli) strain isolated from avian cellulitis lesions. Twenty-two E. Coli isolates were harvested from cellulitis lesions in chicken carcasses in a commercial processing plant. Isolates were then submitted to virulence genotypic profile (iutA, hlyF, iss, ironN, ompT) analysis, antimicrobial susceptibility test, and detection of ESBL production. The results showed that 22.7% of the isolates presented five virulence genes, 9.1% four genes, 36.4% three genes, 13.6% two genes, and 18.2% one gene. The tested isolates showed resistance to ampicillin (90.9%), ceftiofur (54.5%), gentamicin (45.5%), tetracycline (72.1%), sulfamethoxazole/trimethoprim (54.5%), and enrofloxacin (54.5%). Furthermore, 77.3% of the isolates presented multidrug resistance (MDR) profile and 72.7% were positive for ESBL production. This study is the first description of ESBL-producing APEC isolated from avian cellulitis lesions, which suggests the need to establish efficient APEC control measures and programs to prevent flock productivity losses due to colibacillosis and public health risks.

Crescimento comparativo e tolerância ao calor de cabritos de diferentes grupos genéticos no Estado do Rio de Janeiro / Comparative growth and heat tolerance of crossbred goats in Rio de Janeiro State

This study evaluated the comparative growth of 125 feedlot goats of distinct genetic groups (GG): 34 ½ Saanen (SA) + ½ Parda Alpine (PA), 33 ½ SA + ½ Anglo-Nubian (AN), 30 ¾ SA + » PA, and 28 three cross ½ Boer (BO) + » AN + » SA. There was a significative difference (P<0.01) in birth weight (BW), weaning weight (WW), and slaughter weight (SW) between GG. The BW, WW and SW were significantly influenced (P<0.01) by kid sex and birth type. There was a significant difference (P<0.01) between pre-weaning and post-weaning daily weight gain. The weight gain during these periods was significantly influenced (P<0.01) by GG, kid sex and birth type. Pre-weaning aver-age daily weight gains were higher (P<0.01) compared to post-weaning. There was a difference in body temperature (BT), respiratory rate (RR) and heart rate (HR) between GG in the morning (P<0.05) and in the afternoon (P<0.01). The ½ SA + ½ AN and three cross goats had lower BT, RR and HR than ½ SA + ½ PA and ¾ SA + » PA animals. Application of the heat tolerance index proposed by Baccari Junior showed a higher index for ½ SA + ½ AN and three cross goats compared to ½ SA + ½ PA and ¾ SA + ½ PA animals, demonstrating that the former are more tolerant to the climate conditions of the Baixada Fluminense, Rio de Janeiro State.

Foi avaliado o crescimento comparativo de 125 cabritos de diferentes grupos genéticos (GG), sendo 34 ½ Saanen (SA) + ½ Parda Alpina (PA), 33 ½ SA + ½ Anglo-nubiana (AN), 30 ¾ SA + » PA e 28 three cross ½ Bôer (BO) + » AN » + » SA, confinados. A análise estatística revelou diferença (P<0,01) entre os GG, entre peso ao nascer (PN), a desmama (PD) e ao abate (PA). O PN, PD e PA foram influenciados (P<0,01) pelo sexo da cria e tipo de nascimento. Houve diferença (P<0,01) entre o ganho de peso diário no período pré e pós-desmama. Os ganhos de pesos nesses períodos foram influenciados (P<0,01) pelo GG, sexo da cria e tipo de nascimento (P<0,01). Os ganhos de peso médio diário no período pré-desmama foram maiores (P<0,01) em relação ao pós-desmama. Houve diferença na temperatura retal (TR), frequência respiratória (FR) e cardíaca (FC) entre os GG, pela manhã (P<0,05) e a tarde (P<0,01). Os cabritos (½ SA + ½ AN) e o three cross apresentaram a TR, FR e FC mais baixas do que os (½ SA + ½ PA) e (¾ SA + » PA). Pela aplicação do índice de tolerância ao calor (ITC) proposto por Baccari Júnior, os cabritos ½ SA + ½ AN e o three cross obtiveram o ITC mais alto do que o ½ SA + ½ PA e o ¾ SA + ½ PA, revelando-se mais tolerantes às condições climáticas da Baixada Fluminense, Estado do Rio de Janeiro.

Crescimento comparativo e tolerância ao calor de cabritos de diferentes grupos genéticos no Estado do Rio de Janeiro / Comparative growth and heat tolerance of crossbred goats in Rio de Janeiro State

This study evaluated the comparative growth of 125 feedlot goats of distinct genetic groups (GG): 34 ½ Saanen (SA) + ½ Parda Alpine (PA), 33 ½ SA + ½ Anglo-Nubian (AN), 30 ¾ SA + » PA, and 28 three cross ½ Boer (BO) + » AN + » SA. There was a significative difference (P<0.01) in birth weight (BW), weaning weight (WW), and slaughter weight (SW) between GG. The BW, WW and SW were significantly influenced (P<0.01) by kid sex and birth type. There was a significant difference (P<0.01) between pre-weaning and post-weaning daily weight gain. The weight gain during these periods was significantly influenced (P<0.01) by GG, kid sex and birth type. Pre-weaning aver-age daily weight gains were higher (P<0.01) compared to post-weaning. There was a difference in body temperature (BT), respiratory rate (RR) and heart rate (HR) between GG in the morning (P<0.05) and in the afternoon (P<0.01). The ½ SA + ½ AN and three cross goats had lower BT, RR and HR than ½ SA + ½ PA and ¾ SA + » PA animals. Application of the heat tolerance index proposed by Baccari Junior showed a higher index for ½ SA + ½ AN and three cross goats compared to ½ SA + ½ PA and ¾ SA + ½ PA animals, demonstrating that the former are more tolerant to the climate conditions of the Baixada Fluminense, Rio de Janeiro State.(AU)

Foi avaliado o crescimento comparativo de 125 cabritos de diferentes grupos genéticos (GG), sendo 34 ½ Saanen (SA) + ½ Parda Alpina (PA), 33 ½ SA + ½ Anglo-nubiana (AN), 30 ¾ SA + » PA e 28 three cross ½ Bôer (BO) + » AN » + » SA, confinados. A análise estatística revelou diferença (P<0,01) entre os GG, entre peso ao nascer (PN), a desmama (PD) e ao abate (PA). O PN, PD e PA foram influenciados (P<0,01) pelo sexo da cria e tipo de nascimento. Houve diferença (P<0,01) entre o ganho de peso diário no período pré e pós-desmama. Os ganhos de pesos nesses períodos foram influenciados (P<0,01) pelo GG, sexo da cria e tipo de nascimento (P<0,01). Os ganhos de peso médio diário no período pré-desmama foram maiores (P<0,01) em relação ao pós-desmama. Houve diferença na temperatura retal (TR), frequência respiratória (FR) e cardíaca (FC) entre os GG, pela manhã (P<0,05) e a tarde (P<0,01). Os cabritos (½ SA + ½ AN) e o three cross apresentaram a TR, FR e FC mais baixas do que os (½ SA + ½ PA) e (¾ SA + » PA). Pela aplicação do índice de tolerância ao calor (ITC) proposto por Baccari Júnior, os cabritos ½ SA + ½ AN e o three cross obtiveram o ITC mais alto do que o ½ SA + ½ PA e o ¾ SA + ½ PA, revelando-se mais tolerantes às condições climáticas da Baixada Fluminense, Estado do Rio de Janeiro.(AU)