Qualidade da água natural produzida em uma indústria no município de São José de Ribamar – MA / Quality of natural water produced in an industry the municipality of São José de Ribamar – MA

O presente trabalho teve por objetivo avaliar a qualidade físico-química e microbiológica da água natural de uma indústria produtora localizada no município de São José de Ribamar - MA e a implantação das Boas Práticas de Fabricação (BPF). Foram coletadas amostras de água em pontos específicos da indústria sendo eles: poço, filtro I tipo bag, filtro II tipo bag, filtro III tipo polidor, reservatório I, reservatório II, filtro IV tipo polidor, área de envase e laboratório, e realizadas análises microbiológicas por meio do teste rápido Colilert® (pesquisa de coliformes a 35ºC e Escherichia coli) e análises físicoquímicas (condutividade elétrica, potencial hidrogeniônico, sólidos totais e turbidez). A aplicação do checklist foi baseado na Resolução RDC n° 173 de setembro de 2006 da Agência Nacional de Vigilância Sanitária (ANVISA). O resultado das análises físico-químicas e microbiológicas apresentaram-se dentro dos padrões estabelecidos pela RDC nº 274 de 22 de setembro de 2005 da ANVISA.

The aim of the present work was to evaluate a physical-chemical and microbiological quality of natural water industries from one located in the city of São José de Ribamar - MA and the evaluation of Good Manufacturing Practices (GMP’s). Water collections were done at several specific points in the industry, namely: well, filter I type bag, filter II type bag, filter III type polisher, reservoir I, reservoir II, filter IV type polisher, filling area and laboratory, and microbiological analysis were performed using the Colilert® rapid test (research of 35ºC coliforms as well as Escherichia coli) and physical-chemical analysis (electrical conductivity, hydrogen potential, total solids and turbidity). The application of checklist was based in the Resolution RDC No . 173 of September 2006 from ANVISA. The result of analyzes physical-chemical and microbiological are within normal limits and in accordance with the parameters changed by the RDC legislation No . 274/2005 of National Health Surveillance Agency (ANVISA).

Qualidade da água natural produzida em uma indústria no município de São José de Ribamar MA / Quality of natural water produced in an industry the municipality of São José de Ribamar MA

O presente trabalho teve por objetivo avaliar a qualidade físico-química e microbiológica da água natural de uma indústria produtora localizada no município de São José de Ribamar - MA e a implantação das Boas Práticas de Fabricação (BPF). Foram coletadas amostras de água em pontos específicos da indústria sendo eles: poço, filtro I tipo bag, filtro II tipo bag, filtro III tipo polidor, reservatório I, reservatório II, filtro IV tipo polidor, área de envase e laboratório, e realizadas análises microbiológicas por meio do teste rápido Colilert® (pesquisa de coliformes a 35ºC e Escherichia coli) e análises físicoquímicas (condutividade elétrica, potencial hidrogeniônico, sólidos totais e turbidez). A aplicação do checklist foi baseado na Resolução RDC n° 173 de setembro de 2006 da Agência Nacional de Vigilância Sanitária (ANVISA). O resultado das análises físico-químicas e microbiológicas apresentaram-se dentro dos padrões estabelecidos pela RDC nº 274 de 22 de setembro de 2005 da ANVISA.(AU)

The aim of the present work was to evaluate a physical-chemical and microbiological quality of natural water industries from one located in the city of São José de Ribamar - MA and the evaluation of Good Manufacturing Practices (GMPs). Water collections were done at several specific points in the industry, namely: well, filter I type bag, filter II type bag, filter III type polisher, reservoir I, reservoir II, filter IV type polisher, filling area and laboratory, and microbiological analysis were performed using the Colilert® rapid test (research of 35ºC coliforms as well as Escherichia coli) and physical-chemical analysis (electrical conductivity, hydrogen potential, total solids and turbidity). The application of checklist was based in the Resolution RDC No . 173 of September 2006 from ANVISA. The result of analyzes physical-chemical and microbiological are within normal limits and in accordance with the parameters changed by the RDC legislation No . 274/2005 of National Health Surveillance Agency (ANVISA).(AU)

Short-term preservation of Pecari tajacu ovarian preantral follicles using phosphate buffered saline (PBS) or powdered coconut water (ACP(r)) media / Preservação de folículos ovarianos pré-antrais de Pecari tajacu por curtos períodos utilizando meios à base de solução salina fosfatada tamponada (PBS) ou água de coco em pó (ACP(r))

We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed in 5 L Styrofoam boxes, stored for 4h, 12h, 24h, and 36h, and then analyzed. For viability analysis a pair of ovaries from two additional females was divided into nine fragments; one fragment was immediately destined for viability analysis (Trypan blue test) and the other fragments were stored as previously described, until 24h and then analyzed. After 4h storage in ACP(r) medium, the follicular integrity was similar to control (87.8% vs 94.4%, respectively); however, ultrastructural analyses revealed swollen mitochondria as the first signals of PF degeneration. It was observed that ACP(r) (66.7%) was more efficient than PBS (49.4%) to preserve the morphological integrity after 36h storage (P<0.05); however, no differences were observed on follicular viability (P>0.05). In conclusion, the use of the ACP(r) is recommended for the short-term preservation of Pecari tajacu preantral follicles.(AU)

Compararam-se protocolos para a preservação por curtos períodos de folículos ovarianos pré-antrais (PFs) de catetos, utilizando meios à base de solução salina tamponada (PBS) ou água de coco em pó (ACP(r)). Para a análise morfológica, cada par de ovários coletados de seis fêmeas foi dividido em nove fragmentos. Um fragmento foi destinado para a análise da morfologia (histologia e microscopia eletrônica de transmissão - MET), constituindo o grupo controle, e os demais fragmentos foram colocados em tubos contendo PBS ou ACP(r), acondicionados em caixas térmicas de poliestireno expandido de 5L, armazenados durante quatro, 12, 24 e 36 horas, e, então, analisados. Para a análise da viabilidade, pares de ovários de duas fêmeas adicionais foram divididos em nove fragmentos; um deles foi imediatamente destinado à análise da viabilidade (teste com azul de Trypan), os outros fragmentos foram armazenados como descrito previamente até 24h e, então, foram analisados. Após quatro horas de armazenamento em meio ACP(r), a integridade folicular foi similar ao grupo controle (87,8% vs. 94,4%, respectivamente); contudo, a análise ultraestrutural revelou mitocôndrias edemaciadas como os primeiros sinais de degeneração dos PFs. Foi observado que o ACP(r) (66,7%) foi mais eficiente do que o PBS (49.4%) em preservar a integridade morfológica após 36h (p<0,05); entretanto, nenhuma diferença foi observada para a viabilidade folicular (P>0,05). Em conclusão, o uso da ACP(r) é recomendado para a preservação por curtos períodos de folículos pré-antrais de Pecari tajacu.(AU)

Effects of a-MEM and TCM-199 culture media and epidermal growth factor on survival and growth of goat and sheep preantral follicles cultured in vitro

The aim of this study was to evaluate the effect of culture media (Alpha Minimum Essential Medium, α-MEM; and Tissue Culture Medium-199, TCM-199) in the absence or presence of Epidermal Growth Factor (EGF) on an in vitro culture of goat and sheep preantral follicles enclosed in ovarian tissue. The fragments of ovarian cortex from both species were immediately analyzed after collection (non-cultured control group) or cultured for 1 or 7 days in α-MEM+ or TCM-199+in the absence or presence of EGF (10 ng/ml). Before and after the culture, the fragments of ovarian cortex were analyzed by classical histology and fluorescence microscopy. After 1 day of culture, all treatments decreased the percentage of morphologically normal follicles when compared to non-cultured control in both species (P 0.05). Regarding follicle activation, an increase in the percentage of growing follicles was observed in all treatments after 7 days of culture when compared to the control group in both species. However, in sheep, after 7 days, only the treatments α-MEM+/EGF and TCM-199+showed larger(P < 0.05) oocytes than the control group. In conclusion, the TCM-199+ Preserved goat preantral follicle viability after in vitro culture. Furthermore, the media α-MEM+/EGF and TCM-199+ increased the oocyte diameter after 7 days of culture in sheep. Therefore, it isrecommended to use TCM-199+ In the culture of preantral follicles in both species.

Effects of a-MEM and TCM-199 culture media and epidermal growth factor on survival and growth of goat and sheep preantral follicles cultured in vitro

The aim of this study was to evaluate the effect of culture media (Alpha Minimum Essential Medium, α-MEM; and Tissue Culture Medium-199, TCM-199) in the absence or presence of Epidermal Growth Factor (EGF) on an in vitro culture of goat and sheep preantral follicles enclosed in ovarian tissue. The fragments of ovarian cortex from both species were immediately analyzed after collection (non-cultured control group) or cultured for 1 or 7 days in α-MEM+ or TCM-199+in the absence or presence of EGF (10 ng/ml). Before and after the culture, the fragments of ovarian cortex were analyzed by classical histology and fluorescence microscopy. After 1 day of culture, all treatments decreased the percentage of morphologically normal follicles when compared to non-cultured control in both species (P < 0.05). In fluorescence microscopy, viable sheep follicles were observed to decrease in all treatments after 7 days of culture when compared to non-cultured controls. However, in goats, the culture with TCM-199+maintained follicle viability after 7 days of culture, similar to fresh tissue (P > 0.05). Regarding follicle activation, an increase in the percentage of growing follicles was observed in all treatments after 7 days of culture when compared to the control group in both species. However, in sheep, after 7 days, only the treatments α-MEM+/EGF and TCM-199+showed larger(P < 0.05) oocytes than the control group. In conclusion, the TCM-199+ Preserved goat preantral follicle viability after in vitro culture. Furthermore, the media α-MEM+/EGF and TCM-199+ increased the oocyte diameter after 7 days of culture in sheep. Therefore, it isrecommended to use TCM-199+ In the culture of preantral follicles in both species.(AU)