Efeitos da castração imunológica no epitélio seminífero de machos bubalinos / Effects of immunological castration on the seminipheral epithelium of buffaloes males

O procedimento de imunocastração vem sendo utilizado como técnica alternativa amplamente favorável ao bem-estar animal, por ser indolor, pouco invasiva e com eficácia semelhante à da castração cirúrgica, que causa maior estresse aos animais, principalmente quando realizada de forma inadequada. A imunocastração estimula a produção de anticorpos contra o Hormônio Liberador de Gonadotrofina (GnRH), bloqueando temporariamente a produção da testosterona pelas gônadas masculinas. Face a escassez de informações sobre este procedimento em touros bubalinos, o estudo teve como objetivo analisar os possíveis efeitos da imunocastração no parênquima testicular de búfalos. Vinte touros bubalinos, com idade entre 2 a 3 anos, oriundos da ilha do Marajó ­ PA, foram avaliados e divididos aleatoriamente em dois grupos composto por dez animais no grupo controle (GC) e dez do grupo imunocastrado (GIM). O produto utilizado foi a vacina anti-GnRH Bopriva ® (Zoetis, SP, Brasil). O GIM recebeu duas doses de 1,0ml contendo 400µg da vacina Bopriva ® com intervalo de 8 semanas entre as aplicações e o grupo controle recebeu 1,0 ml de solução fisiológica. Após 14 dias da última dose, os animais foram abatidos e coletado os 20 pares de testículos para analises de parâmetros macroscópicos como: comprimento, largura, circunferência e peso. Além disso, foi realizada a retirada de fragmentos do parênquima testicular para confecção de lâminas histológicas. Para comparação dos dados foi aplicado o teste t de Student (Nonparametric Test) considerando a significância com p<0,05 e intervalo de confiança de 95%. Foi observado diferença significativa entre os dois grupos para grupos para peso (direto p<0001; esquerdo p≤ 0,0001), circunferência (direita p< <0,0001), 1; esquerda p<0,0001), ), largura (somente lado direito p<0,0372) e comprimento (direita p≤ 0,0013; esquerda p<0,0437). Na avaliação microscópica, os animais do GC não apresenteram alterações. Nos animais do GIM houve degeneração em todas as amostras, sendo visualizado descamação, tortuosidade e espessamento da membrana basal do túbulo seminífero, assim como vacuolização e atrofia das células de Sertoli. Foi observado também uma redução do número de células de Leydig, fibrose intertubular pronunciada, núcleos picnóticos, azoospermia e células multinucleadas no interior dos túbulos. Conclui-se a vacina anti-GnRH mostrou-se eficaz em provocar lesões no parênquima testicular, comprometendo significativamente a espermatogênese a partir da possível supressão de testosterona.(AU)

The immunocastration procedure has been used as an alternative technique widely favorable to animal welfare, as it is painless, minimally invasive and with similar efficacy to surgical castration, which causes greater stress to animals, especially when performed improperly. Immunocastration stimulates the production of antibodies against Gonadotropin Releasing Hormone (GnRH), temporarily blocking the production of testosterone by the male gonads. Due to the scarcity of information about this procedure in buffalo bulls, the study aimed to analyze the possible effects of immunocastration on the testicular parenchyma of buffaloes. Twenty buffalo bulls, aged between 2 and 3 years, from the island of Marajó - PA, were evaluated and randomly divided into two groups consisting of ten animals in the control group (CG) and ten from the immunocastrated group (GIM). The product used was the anti-GnRH vaccine Bopriva ® (Zoetis, SP, Brazil). The GIM received two doses of 1.0ml containing 400µg of Bopriva ® vaccine with an interval of 8 weeks between applications and the control group received 1.0 ml of saline solution. After 14 days of the last dose, the animals were slaughtered and the 20 pairs of testes were collected for analysis of macroscopic parameters such as: length, width, circumference and weight. In addition, fragments of the testicular parenchyma were removed for the preparation of histological slides. To compare the data, Student's t test (Nonparametric Test) was applied, considering the significance with p< 0.0372) and length (right p≤ 0.0013; left p<0.0437). In the microscopic evaluation, the animals of the CG showed no alterations. In the GIM animals there was degeneration in all samples, with desquamation, tortuosity and thickening of the seminiferous tubule basement membrane, as well as vacuolization and atrophy of Sertoli cells. A reduction in the number of Leydig cells, pronounced intertubular fibrosis, pyknotic nuclei, azoospermia and multinucleated cells within the tubules were also observed. In conclusion, the anti-GnRH vaccine proved to be effective in causing lesions in the testicular parenchyma, significantly compromising spermatogenesis from the possible suppression of testosterone.(AU)

Influência da categoria da vaca sobre a taxa de prenhez em bubalinos (Bubalus Bubalis) / Influence of category of the cow on the pregnancy rate in buffaloes (Bubalus Bubalis)

The objective of this study was to evaluate the rate of pregnancy in cows, buffaloes in differentcategories. The present study was conducted in Agricultural Farm Bike General LTDA. Animals were kept underextensive grazing. 48 animals were used, being 8 females and females 40 Primiparous multiparous subjected toa protocol of artificial insemination in fixed time (IATF). The gestation diagnosis was carried out with 30 daysafter artificial insemination. Of 48 females inseminated, Primiparous cows Group obtained as a result 4 (50%)pregnant women and 4 (50%) not pregnant woman and multiparous cows Group 19 (47.50%) were pregnantwomen and 21 (52.50%) not pregnant women. According to the results obtained in this study by dividing thefemales for Primiparous and multiparous category, the rates of pregnancy in buffaloes was influences by cowcategory, indicating that primiparas in need of greater attention towards their nutritional requirements.(AU)

Perfil da Inseminação artificial em tempo fixo, presença de muco e o status uterino correlacionado com a taxa de prenhez em vacas bubalinas no estado do Amapá / Profile of artificial insemination in fixed time, mucus and uterine status correlated with pregnancy rate in buffalo cows in Amapa state

The aim of this study was to correlate the profile of insemination, uterine status and the presence ofmucus on the day of insemination, with a pregnancy rate of buffalo cows in lowland area in the state of Amapá.This study was conducted at Fazenda general Moto LTDA., 148 km from the town of Macapa. The animals werekept in extensive grazing system. They used 48 animals received the following protocol: Day 0 (D0) to insert theintravaginal device P4 and 2.0 mg BE. At D9 was taken withdrawal of the device and application of 0.5 mg ofPGF2a and 400 IU eCG. In D11 was applied 0.004 mg GRnH and carried out insemination in D12, 64 hoursafter the withdrawal. The diagnosis of pregnancy was performed 30 days after artificial insemination. The datacollected from individual records were tabulated in spreadsheets. And evaluated in the SAS software (2000) 23buffaloes that had a positive pregnancy, 3 a bad AI profile, 18 a good AI profile and 2 excellent AI profile. 25cows got negative pregnancy, among them 4 demonstrated bad AI profile, 21 a good AI profile and no possessedexcellent AI profile. Regarding uterine Status- 46 animals (97.9%) had contracted uterus, however only 22(47.83%) animals were diagnosed with positive penhez. The variable presence of mucus, of 48 buffalos 21(43.75%) had such characteristics. The pregnancy rate was 22.9% (11) for the cows with mucus during theIATF. The variables profile of AI, mucus, uterine status, no positive influence on the pregnancy rate.(AU)

Influência da categoria da vaca sobre a taxa de prenhez em bubalinos (Bubalus Bubalis) / Influence of category of the cow on the pregnancy rate in buffaloes (Bubalus Bubalis)

The objective of this study was to evaluate the rate of pregnancy in cows, buffaloes in differentcategories. The present study was conducted in Agricultural Farm Bike General LTDA. Animals were kept underextensive grazing. 48 animals were used, being 8 females and females 40 Primiparous multiparous subjected toa protocol of artificial insemination in fixed time (IATF). The gestation diagnosis was carried out with 30 daysafter artificial insemination. Of 48 females inseminated, Primiparous cows Group obtained as a result 4 (50%)pregnant women and 4 (50%) not pregnant woman and multiparous cows Group 19 (47.50%) were pregnantwomen and 21 (52.50%) not pregnant women. According to the results obtained in this study by dividing thefemales for Primiparous and multiparous category, the rates of pregnancy in buffaloes was influences by cowcategory, indicating that primiparas in need of greater attention towards their nutritional requirements.

Perfil da Inseminação artificial em tempo fixo, presença de muco e o status uterino correlacionado com a taxa de prenhez em vacas bubalinas no estado do Amapá / Profile of artificial insemination in fixed time, mucus and uterine status correlated with pregnancy rate in buffalo cows in Amapa state

The aim of this study was to correlate the profile of insemination, uterine status and the presence ofmucus on the day of insemination, with a pregnancy rate of buffalo cows in lowland area in the state of Amapá.This study was conducted at Fazenda general Moto LTDA., 148 km from the town of Macapa. The animals werekept in extensive grazing system. They used 48 animals received the following protocol: Day 0 (D0) to insert theintravaginal device P4 and 2.0 mg BE. At D9 was taken withdrawal of the device and application of 0.5 mg ofPGF2a and 400 IU eCG. In D11 was applied 0.004 mg GRnH and carried out insemination in D12, 64 hoursafter the withdrawal. The diagnosis of pregnancy was performed 30 days after artificial insemination. The datacollected from individual records were tabulated in spreadsheets. And evaluated in the SAS software (2000) 23buffaloes that had a positive pregnancy, 3 a bad AI profile, 18 a good AI profile and 2 excellent AI profile. 25cows got negative pregnancy, among them 4 demonstrated bad AI profile, 21 a good AI profile and no possessedexcellent AI profile. Regarding uterine Status- 46 animals (97.9%) had contracted uterus, however only 22(47.83%) animals were diagnosed with positive penhez. The variable presence of mucus, of 48 buffalos 21(43.75%) had such characteristics. The pregnancy rate was 22.9% (11) for the cows with mucus during theIATF. The variables profile of AI, mucus, uterine status, no positive influence on the pregnancy rate.

Intervalo entre partos de Búfalas Murrah e Mestiças submetidas à monta natural e inseminação artificial no município de Taipu, Rio Grande do Norte / Interval between births of Buffaloes Murrah and Gostosas submitted to monta natural and artificial insemination in the municipality of Taipu, Rio Grande do Norte

This study aimed to avaluate the interval between calvins (IBC) from a buffalo herd, and to evaluate theinfluence of the blood level and the reproduction system in the IBC. Four hundred and one data were analyzedbetween 2000 and 2015 of buffalo females, Murrah breed, and dairy mixed breeds maintained in an intensivesystem and submitted to two daily milking. Statistical data were evaluated in the SAS software (2010), for theanalysis of the different experimental conditions, analysis of variance (ANOVA) and Tukey test with significancelevel of 5%. The mean value found for IBC was 425,9±66,1 days, the AFC mean value according to blood levelof Murrah breed and mixed breed was respectively 427,4 ± 65,5 and 418,4 ± 69,6, (P > 0,05). Consideringbreed systems artificial insemination and natural-service the IBC was 434,3 ± 55,6 and 424,1 ± 68,2respectively, (P > 0,05). Therefore, there was no influence of the blood level and breeding system on IBC.(AU)

Idade ao primeiro parto de Búfalas Murrah e Mestiças criadas no município de Taipu, estado do Rio Grande do Norte / Age at first birth Buffaloes Murrah and Crossbred Maids in the city of Taipu, Rio Grande do Norte state

This study aimed to evaluate the age of the first calving of buffaloes (AFC) from a buffalo herd and toevaluate the influence of the blood level in the AFC. Five hundred and eight data were analyzed between 2000and 2015 of buffalo females, Murrah breed, and dairy mixed breeds maintained in an intensive system andsubmitted to two daily milking. Statistical data were evaluated in the SAS software (2010), for the analysis of thedifferent experimental conditions, analysis of variance (ANOVA) and Tukey test with significance level of 5%.The mean value found for AFC was 38.9 ± 5.5 months (1170 days), the AFC mean value according to blood levelof Murrah breed and mixed breed was respectively 38.9 ± 5.5 months and 38.90 ± 5.3 months, (P > 0.05).Therefore, there was no influence of the blood level in the AFC.(AU)

Influência da presença do corpo lúteo na taxa de prenhez em búfalas submetidas à IATF / Influence of corpus luteum presence in the pregnancy rate in buffaloes submitted to TAI

This study evaluated the influence of the presence or absence of corpus luteum at the beginning ofartificial insemination protocol at fixed time (TAI) in pregnancy rate. 48 animals were used which prior toinsertion of releasing progesterone device were evaluated by rectal palpation for the presence or absence of thecorpus luteum, and divided into two groups: G1 cows with the presence of the corpus luteum, and G2 cows notwithout palpable structures. The data collected from individual records were tabulated in spreadsheets andevaluated in the SAS software (2000). The pregnancy rate for G1 and G2 was 40% 53.57% respectively, P >0.05. Suggesting that the presence of corpus luteum did not influence the pregnancy rate in TAI.(AU)

Taxa de prenhez em matrizes suínas submetidas aos métodos de inseminação artificial convencional e monta natural em granja comercial no nordeste paraense / Pregnancy rate in sows submitted to the methods of Artificial Insemination Conventional and Natural Monta in commercial farm in northeast Pará

The reproductive planning a squad, using natural breeding (MN), can be carried out to the maximumuse of animals (Campos, 2008). However there are practical to provide improvements in the system, such asartificial insemination (AI). twelve were used sows with an average age of two years. Pregnancy diagnosis wasperformed 30 days after the procedure. The arrays were divided into two groups, six were subjected to the IA-six(MN). The pregnancy rate was 66.67% and the rate of return to estrus was 33.33% for both treatments (P>0.05), with no significant difference. The AI in pigs shows satisfactory results in the northeast of Pará, whichprovides the producer, recognize that the method is the most profitable because it reduces the number of animalsin the herd, reducing environmental impacts and risks of transmission of sexually transmitted diseases.(AU)

Ocorrência de esterilização por conveniência em animais domésticos atendidos no HOVET/ UFRA região metropolitana de Belém PA / Sterilization occurrence for convenience in domestic animals treated at HOVET / UFRA metropolitan area of Belém - PA

It aims to show some reasons to castrate a domestic animal such as the regulation of the population offree-living cats or confined, elimination of unpleasant behaviors such as fighting, wandering behavior and sprayurine, mainly shown by adult males. Currently affects companies in the animal health sector, nutrition,equipment, accessories, expertise of veterinarians, publications, services, among others. But there is still greatshortage of professionals focused on this very special market, where literature is scarce, as well as theknowledge Furtado. The survey was conducted at the Veterinary Federal Rural University of Amazonia Hospital(UFRA), Campus Belém / Pará, with animals attended by residents of the Animal Reproduction Department(SRA), the cat from Bethlehem.(AU)

Ocorrência de patologias da glândula mamária e distúrbios do parto em fêmeas felinas atendidas no Hospital Veterinário da Universidade Federal Rural da Amazônia / Occurrence of mammary gland diseases and Postpartum disorders in feline females met at the Veterinary Hospital of Universidade Federal Rural da Amazônia

The feline clinic is increasing in worldwide and in Brazil. The reproductive systems pathologies of catsare frequent in veterinary medicine; they featuring with complex and multifactorial causes, both in females andmales, with varying degrees of morbidity and mortality, and it has few studies about. This paper accomplished aresearch of reproductive occurrences in domestic cats, they attended by the Animal Reproduction Department(SRA) of the Veterinary Hospital, located in Federal Rural University of Amazonia (UFRA). In the period ofMarch 1, 2013 to 18 December 2015, it were 472 cats attended from the Belems metropolitan area, they being432 females and 40 males, which was related the factors breed, fur, gender and age. Most animals wereanimals mongrel and adult age group, mostly females affected in different reproductive organs, especially themammary glands with the high rate of breast tumors (24, 54%) and obstetric disorders.(AU)

Ocorrência de patologias de útero, ovário, vagina e diagnósticos fisiológicos em fêmeas felinas atendidas no Hospital Veterinário da Universidade Federal Rural da Amazônia / Occurrence of diseases of uterus, ovary, vagina and physiological diagnostics in feline females met at the Veterinary Hospital of Universidad Federal Rural da Amazonia

The pathologies that affect the reproductive systems are occurring more frequently in veterinarymedicine, because diseases are multifactorial, so may be regional variations in the incidence of certainreproductive abnormalities. This work was conducted a survey of breeding occurrences in domestic cats, theyparticipated in the Department of Animal husbandry (SRA) of the Veterinary Hospital, located at UniversidadeFederal Rural da Amazônia (UFRA). In the period of 1 March 2013 to 2015, 18 December was 472 catsparticipated in the metropolitan area of Bethlehem, they being 432 females and 40 males, which was related tothe factors of race, gender, and age. Most of the animals were stray animals and adult age group, mostlyfemales affected in different reproductive organs, with disease in the uterus and ovaries and occurrence as rarecases of cystic Dysplasia, puerperal metritis, stump pyometra, ovarian cancer and endometrial polyps. Inaddition, there was interest in animals to seek prenatal diagnosis of pregnancy of animals.(AU)

Ocorrência de patologias em machos felinos atendidos no Hospital Veterinário da Universidade Federal Rural da Amazônia / Occurrence of diseases in cats males treated at the Veterinary Hospital of the Federal Rural University of Amazonia

The pathologies of the reproductive system of cats are common in veterinary medicine and are alwayscomplex and multifactorial, where the primary objective is to determine the cause of the problem, both infemales as in males, with varying degrees of morbidity, mortality and suffering influences reproductive historyfrom previous pharmacological treatments and environmental conditions and can therefore be regionalvariations in the incidence of certain reproductive abnormalities. Cryptorchidism is incomplete testiculardescent, may be retained in any follow this path. The survey was conducted at the Veterinary Hospital (HOVET /UFRA), Belém / Pará, with animals attended by residents of Animal Reproduction Section, feline fromBethlehem. The information on the animals were obtained by consulting the records contained in the files theSISVET (veterinary System) program being evaluated only attended by veterinary medical residents of the Sectorof Small Animal Reproduction Clinic of this hospital.(AU)

Piometra de cérvix fechada em canina da raça Pinscher: Relato de Caso / Closed cervix Pyometra in a Pinscher: Case Report

The cystic endometrial hyperplasia pyometra complex, is a frequent and important endometrialdisorder in bitches, since it presents a risk to the lives of these animals. It tends to occur in middle-aged to olderbitches. This paper aims to report a case of closed pyometra in a Pinscher dog. On 29 of August of 2016, a fiveyear-oldPinscher dog weighing 5.7 kg was taken to the Veterinary Hospital of the Universidade Federal Ruralda Amazônia (HOVET / UFRA), due to an one year increase and decrease in abdominal region, but two monthsago it increased in size and did not regress. The animal was diagnosed by US and historical examination, withclosed pyometra. The animal recovered well postoperatively. It is important the knowledge about the animal'shistory and diagnosis by ultrasound imaging in order to conclude the diagnosis, and thus to perform quick andeffective surgical intervention.(AU)

Taxa de prenhez de acordo com o tipo de muco apresentado em cabras da raça Saanen submetidas a inseminação artificial em tempo fixo (IATF) / Pregnancy rate according to the type of mucus presented in Saanen breed goats submitted to artificial insemination in fixed time (TAI)

This study aimed to evaluate the pregnancy rate according to the type of mucus presented in Saanengoats submitted to artificial insemination in fixed time (TAI). The study was conducted in the state of Pará, in themunicipality of Iguarapé-Açu, in Pará Northeast, in a private property. 12 goats used for milk production wereused, with ages ranging from 1 to 3 years, the classification of the type of vaginal mucus, was made according toSmith (2006). The pregnancy rate for animals with crystalline mucus was 33.3% for those who had crystallinestriatedmucus was 0% for those who had striatum mucus was 66.6% for those who had striatum mucus -caseosowas 50% and for those who had mucus cheesy pregnancy rate was 50%. After data analysis showed that the typeof mucus does not influence the animal pregnancy rate.(AU)

Efeito do tratamento com dose reduzida do aglepristone em uma hiperplasia mamaria felina: Relato de caso / Effect of the treatment with low dose of aglepristone in a feline mammary hyperplasia: case report

Feline mammary hyperplasia (FMH) is a benign condition representing a disorder of body related tonatural or synthetic progestogen characterized by rapid cell growth of one or more mammary glands. Havingrapidly evolving and can cause serious systemic changes, it is necessary to use instant protocols, such as the useof aglepristone an antiprogestogen. This study aims to report a case of FMH with aglepristone-based therapy. Acat mongrel, with 9 months old, nulliparous, who received a contraceptive dose, showed an increase in thethoracic right mammary gland M1. The history and physical examination indicated the diagnosis of FMH. Thetherapy established was aglepristone in 3 doses (10mg / kg) every 24 hours. The result was total remission ofsymptoms. The use of aglepristone proved to be effective in the treatment of FMH even without castration.(AU)

Eficiência reprodutiva de fêmeas suínas em sistema de monta natural oriundas de granja comercial no nordeste paraense / Reproductive efficiency of sows in natural mating system originated from commercial farm in northeast Pará

The objective of this study was to evaluate the reproductive efficiency in a commercial farm pig in thecity of Benevides, Pará. We carried out the collection of pregnancy and parturition of the data arrays in twoperiods, and evaluated the rate of return to estrus. 90 arrays of March were accompanied to August 2015 and 81arrays from February to April 2016. Females were multiparous, housed in gestation crates and maternity andwithout food manejo difference. Thus, the feeding mothers in pregnancy and phase of breeding was carried outtwice a day (± 2 kg feed / animal / day). The pregnancy rate was 66.35% referring to 2015 and 57.92% relatedto 2016. Rates of return to estrus were 33.65% and 42.09% for 2015 and 2016, respectively. The reproductiverates of ownership are lower than ideal for productive activity.(AU)

Leiomiossarcoma uterino em Canis familiaris (Linnaeus, 1758): Relato de caso / Uterine leiomyosarcoma in Canis familiaris (Linneus, 1758): a case report

Leiomyosarcomas are rare malignant neoplasias originated from smooth muscle and may affectprimarily female reproductive organs. The aim of this work is to report a case of uterine leiomyosarcoma in thebitch. A female dog, mixed breed, 6 years old showed history of use of contraceptive and it underwent to aelective ovariosalpingohysterectomy. Multiple uterine masses were observed grossly and the histopathologyconfirmed a uterine leiomyosarcoma associated with cystic endometrial hyperplasia and ovarian cysts.(AU)

Idade ao primeiro parto de Búfalas Murrah e Mestiças criadas no município de Taipu, estado do Rio Grande do Norte / Age at first birth Buffaloes Murrah and Crossbred Maids in the city of Taipu, Rio Grande do Norte state

This study aimed to evaluate the age of the first calving of buffaloes (AFC) from a buffalo herd and toevaluate the influence of the blood level in the AFC. Five hundred and eight data were analyzed between 2000and 2015 of buffalo females, Murrah breed, and dairy mixed breeds maintained in an intensive system andsubmitted to two daily milking. Statistical data were evaluated in the SAS software (2010), for the analysis of thedifferent experimental conditions, analysis of variance (ANOVA) and Tukey test with significance level of 5%.The mean value found for AFC was 38.9 ± 5.5 months (1170 days), the AFC mean value according to blood levelof Murrah breed and mixed breed was respectively 38.9 ± 5.5 months and 38.90 ± 5.3 months, (P > 0.05).Therefore, there was no influence of the blood level in the AFC.

Influência da presença do corpo lúteo na taxa de prenhez em búfalas submetidas à IATF / Influence of corpus luteum presence in the pregnancy rate in buffaloes submitted to TAI

This study evaluated the influence of the presence or absence of corpus luteum at the beginning ofartificial insemination protocol at fixed time (TAI) in pregnancy rate. 48 animals were used which prior toinsertion of releasing progesterone device were evaluated by rectal palpation for the presence or absence of thecorpus luteum, and divided into two groups: G1 cows with the presence of the corpus luteum, and G2 cows notwithout palpable structures. The data collected from individual records were tabulated in spreadsheets andevaluated in the SAS software (2000). The pregnancy rate for G1 and G2 was 40% 53.57% respectively, P >0.05. Suggesting that the presence of corpus luteum did not influence the pregnancy rate in TAI.